Antibacterial slow-release dressing and preparation method thereof
By using a multi-layered antibacterial sustained-release dressing design, and utilizing liquid-guided fibers and a variety of antibacterial agents, the shortcomings of traditional dressings in hemostasis and antibacterial properties are overcome, achieving efficient wound management.
Patent Information
- Application Number
- CN202511058367.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-07-30
- Publication Date
- 2025-12-19
AI Technical Summary
Traditional dressings cannot effectively inhibit bacterial growth in wound exudate, leading to frequent changes and repeated infections, and it is difficult to achieve rapid hemostasis and antibacterial treatment at the same time.
A multi-layered antibacterial sustained-release dressing was designed, comprising a first contact layer, a second contact layer, and a third contact layer, each containing a microporous contact material, a hemostatic agent, an antibacterial agent, chitosan, and a liquid absorbent. Exudate is directed to the third contact layer for absorption and sealing via liquid guiding fibers, and combined with multiple antibacterial agents, it achieves continuous antibacterial action.
It maintains highly effective hemostasis and antibacterial properties when dealing with large amounts of exudate, reduces recurrent infections, and is suitable for rapid closure and transfer of acute trauma.
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Figure CN121154882A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the field of medical dressings, and particularly relates to an antibacterial sustained-release dressing and a preparation method thereof. BACKGROUND
[0002] In the field of medical care, wound infection is a core problem affecting wound healing. According to statistics, the medical cost caused by wound infection worldwide each year exceeds 30 billion US dollars, and about 60% of the infections are caused by the fact that traditional dressings cannot effectively inhibit the growth of bacteria.
[0003] In traditional wound dressing technology, the body fluid exuded from the wound exists in the dressing, which is easy to cause bacterial growth, and the bacteria growing in the dressing will contact the patient's blood through the wound, which causes the dressing of the patient's wound to not only need to be frequently replaced, but even after replacement, it still cannot effectively prevent repeated bacterial infection, which causes the patient's wound to not heal in time, and seriously affects the recovery effect of the patient after the operation.
[0004] Especially worth pointing out is that in some acute trauma cases, a wound with a large amount of bleeding needs to be quickly closed and hemostasis, and then the patient needs to be transferred; in this case, the wound of the patient needs to be simultaneously subjected to quick and convenient hemostasis operation, and at the same time, the wound needs to be subjected to antibacterial treatment to avoid bacterial infection of the wound during the transfer of the patient; in the traditional technology, it is difficult to simultaneously meet these needs.
[0005] Therefore, it is necessary to develop a new antibacterial sustained-release dressing to overcome the problem of insufficient antibacterial performance in traditional dressings. SUMMARY
[0006] The purpose of the present application is to solve the problems of the prior art, and provide an antibacterial sustained-release dressing and a preparation method thereof, which specifically adopt the following technical solutions: Firstly, the present application provides an antibacterial sustained-release dressing, which specifically comprises: a carrier and a contact body, the carrier is used for fixing the contact body on the skin surface and making the contact body directly contact with the human body; The contact body comprises a first contact layer, a second contact layer and a third contact layer from near to far in the order of contacting the human body; The raw material of the first contact layer comprises: a microporous contact material, a hemostatic agent and a first antibacterial agent; The raw material of the second contact layer comprises: chitosan, polyurethane, a second antibacterial agent and a one-way isolation film; The raw material of the third contact layer comprises: a liquid absorbent, a coagulant and a third antibacterial agent; The antibacterial sustained-release dressing further comprises liquid guiding fibers, and the liquid guiding fibers pass through the second contact layer and are embedded in the third contact layer.
[0007] The anti-bacterial sustained-release dressing provided by the application is divided into a multi-layer structure, and the carrier of the dressing bears the multi-layer structure and makes the contact body of the multi-layer dressing adhere to the wound, so that the contact body of the dressing can fully and timely absorb the exudate of the wound; the contact body also comprises a multi-layer structure, the first contact layer is used to adhere to the surface of the wound of the patient, fully contacts the skin wound, and guides the exudate of the wound, so that the exudate can flow into the second contact layer as soon as possible to avoid accumulation of a large amount of liquid on the surface of the wound; the second contact layer is provided with an anti-bacterial blood coagulation material to coagulate a small amount of blood in the liquid flowing in through the first contact layer; when the exudation of the wound is large, the blood in the exudate gradually coagulates in the second layer, and the remaining substances flow into the third contact layer through the guide fibers, the third contact layer is used to absorb part of the liquid to avoid the blood coagulation components in the second contact layer being washed away, and fix the liquid in the third layer; avoid part of the liquid flowing out during the wound hemostasis process from flowing back into the wound, thereby relieving the repeated infection of the wound; furthermore, the sustained-release anti-bacterial component in the dressing can continuously inhibit bacteria.
[0008] Specifically, after the first contact layer of the dressing contacts the liquid, it transports the liquid to the second contact layer and the third contact layer while preliminarily stopping bleeding; the second contact layer transports part of the liquid to the third contact layer while rapidly stopping bleeding, and gradually blocks the channel of the transported liquid and cuts off the reflux path while gradually completing the hemostasis process, so that the blood coagulation material in the second contact layer is not washed away while coagulating blood, thereby ensuring that the dressing can still maintain a high hemostasis effect on the basis of anti-bacterial when facing a large amount of liquid exudation.
[0009] In some specific embodiments, the raw material of the carrier includes at least one of polyester fiber, polyester fiber, polypropylene and cotton fiber. The raw material of the carrier described above enables the carrier to maintain good bearing performance and protection performance. In some preferred specific embodiments, the carrier can also select a material with waterproof function, such as a polytetrafluoroethylene film, to provide better waterproof effect.
[0010] In some specific embodiments, the raw material of the liquid guide fiber includes polylactic acid, polybutylene succinate and polycaprolactone.
[0011] In some specific embodiments, the specific preparation method of the liquid guide fiber comprises the following steps: grafting the raw material of the liquid guide fiber with maleic anhydride and carboxylating treatment, and then electrospinning to obtain the liquid guide fiber described above.
[0012] In some specific embodiments, the raw material of the first antibacterial agent comprises at least one of titanium dioxide, copper oxide, zinc carbonate, zinc oxide and zinc sulfate. Among them, the first antibacterial agent adopts antibacterial titanium ions, copper ions and zinc ions, so as to obtain fast, efficient and safe skin antibacterial performance.
[0013] In some specific embodiments, the raw material of the second antibacterial agent comprises a cellulose derivative and a second antibacterial core component, and the second antibacterial core component comprises at least one of tea polyphenol, lysozyme and nisin. In the second contact layer, sustained antibacterial slow release is required, and the second antibacterial agent provided by the present application has slow release performance and can meet the long-term antibacterial effect.
[0014] In some specific embodiments, the preparation method of the second antibacterial agent comprises the following steps: dissolving the cellulose derivative in water, adding polyvinyl alcohol and mixing at 75-85℃, then adding the second antibacterial core component, mixing and heating for 20-40min; after heating, cooling is carried out, and the cooling time is 3-7min per 1℃, then precipitation is carried out, and the second antibacterial agent is obtained. Among them, the second antibacterial agent prepared by the specific preparation method not only has excellent antibacterial performance, but also has excellent mixing effect with other components in the second contact layer, and is convenient for full spraying, so that the comprehensive effect of the second contact layer is greatly improved.
[0015] In some specific embodiments, the raw material of the third antibacterial agent comprises staphyloglycolipase and lactic acid bacteria lysozyme. The third antibacterial agent is used to prevent the liquid fixed therein from growing a large number of bacteria, so that the antibacterial slow-release dressing provided by the present application can maintain excellent antibacterial ability.
[0016] In some specific embodiments, the microporous contact material comprises a microporous non-woven fabric. The liquid absorption performance of the non-woven fabric is more suitable for the antibacterial slow-release dressing provided by the present application.
[0017] Secondly, the application further provides a preparation method of the above-mentioned antibacterial sustained-release dressing, specifically comprising the following steps: preparing a carrier, gluing the carrier to obtain a carrier with a glued surface; implanting liquid guiding fibers for the first time to bond the liquid guiding fibers to the glued surface; then spraying the third contact layer for the first time, after the first spraying is completed, implanting liquid guiding fibers for the second time, and then spraying the third contact layer for the second time; after the second spraying is completed, spraying the one-way isolation film, after the spraying of the one-way isolation film is completed, spraying the raw material of the second contact layer containing chitosan, polyurethane and the second antibacterial agent on the one-way isolation film, then cutting the liquid guiding fibers and performing flat pressing to obtain the carrier with the second contact layer and the third contact layer; then, the first contact layer is pressed with the carrier with the second contact layer and the third contact layer to obtain the antibacterial sustained-release dressing.
[0018] In the specific preparation method of the antibacterial sustained-release dressing provided by the application, the liquid guiding fibers form a liquid guiding structure, so that part of the liquid flows into the third contact layer, avoiding a large amount of liquid from flowing into the second contact layer to cause the coagulation function of the second contact layer to fail; at the same time, when the blood coagulation is completed, the liquid guiding fibers are fixed synchronously, and the coagulated blood fully wraps the liquid guiding fibers to block the liquid from flowing into the third contact layer, avoiding continuous blood loss.
[0019] In some specific embodiments, the preparation method of the first contact layer comprises the following steps: dissolving the hemostatic agent and the first antibacterial agent in a solvent to prepare a first contact layer loading solution, and immersing the microporous contact material in the first contact layer loading solution. In the first contact layer, the microporous contact material can be combined with the hemostatic agent and the first antibacterial agent to the maximum extent by immersion, so that the rapid antibacterial effect of the first contact layer is maximized.
[0020] In some specific embodiments, in the preparation method, when the liquid guiding fibers are cut, the exposed length of the liquid guiding fibers is 0.5 mm-2 mm. The liquid guiding fibers with an appropriate length can balance the cost and effect.
[0021] The application has the beneficial effects that the application provides an antibacterial sustained-release dressing. The antibacterial sustained-release dressing has a multi-layer antibacterial structure with liquid guiding, storage and blocking functions on the carrier, so that the antibacterial performance of the dressing is obviously improved, and the dressing can be applied to a large amount of exudate and a small amount of exudate. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 FIG. 1 is a liquid guiding fiber electron microscope image of an antibacterial sustained-release dressing provided in Example 1 in the specification of the application; Figure 2A structural schematic diagram of an antibacterial sustained-release dressing provided in the specific embodiments in the specification of the present application is shown. Figure 3 A photo of an antibacterial sustained-release dressing provided in Example 1 in the specification of the present application is shown. Figure 4 A photo of an antibacterial sustained-release dressing provided in Example 1 in the specification of the present application is shown. Figure 5 Photos of mice wounds in a wound healing experiment of each sample provided by Example 1, Example 2, Comparative Example 1, Comparative Example 2 and the blank group in the specification of the present application are shown. DETAILED DESCRIPTION
[0023] The concept, specific structure and technical effects of the present application will be described clearly and completely in combination with the embodiments and the drawings to fully understand the purpose, scheme and effects of the present application. It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The same reference signs used in the drawings indicate the same or similar parts.
[0024] Example 1 First, the present embodiment provides an antibacterial sustained-release dressing, specifically comprising: a carrier and a contact body, the carrier is used to fix the contact body on the skin surface and make the contact body directly contact with the human body; The contact body includes a first contact layer, a second contact layer and a third contact layer from near to far in the distance of contacting the human body; The raw material of the first contact layer includes: a microporous contact material, a hemostatic agent and a first antibacterial agent, the microporous contact material is a non-woven fabric, the hemostatic agent is thrombin, and the first antibacterial agent is copper oxide and zinc oxide with a mass ratio of 1:1; The raw material of the second contact layer includes: chitosan, polyurethane, a second antibacterial agent and a one-way isolation film, wherein the raw material of the second antibacterial agent is a cellulose derivative and a second antibacterial core component, and the second antibacterial core component is tea polyphenol, lysozyme and streptococcal nuclease; the cellulose derivative is specifically carboxymethyl cellulose; The specific preparation method of the second antibacterial agent is: carboxymethyl cellulose is dissolved in an aqueous solution, polyvinyl alcohol is added and mixed uniformly at 75 ℃, then the second antibacterial core component is added, and after mixing, heating is performed for 25 min; after completing the heating, cooling is performed, and the cooling time is 3 min per 1 ℃, and then precipitation is performed to obtain the second antibacterial agent.
[0025] The raw material of the third contact layer includes: a liquid absorbent, a coagulant and a third antibacterial agent, wherein the liquid absorbent is sodium carboxymethyl cellulose, the coagulant is hemagglutinin, and the third antibacterial agent is staphylococcal nuclease and lactic acid bacterial nuclease; The antibacterial sustained-release dressing also comprises liquid guide fibers made of polylactic acid, and the preparation method comprises grafting polylactic acid with maleic anhydride and performing carboxylation treatment, and then performing electrospinning; in the antibacterial sustained-release dressing provided in the embodiment, the liquid guide fibers pass through the second contact layer and are embedded in the third contact layer.
[0026] Secondly, the embodiment also provides a specific preparation method of the antibacterial sustained-release dressing, and the steps are as follows: A carrier is prepared, the carrier is glued to obtain a carrier with a glued surface, the first liquid guide fiber implantation is performed to bond the liquid guide fibers to the glued surface, then the first spraying of the third contact layer is performed, after the first spraying, the second liquid guide fiber implantation is performed, and then the second spraying of the third contact layer is performed; after the second spraying, the spraying of the one-way isolation film is performed, after the spraying of the one-way isolation film, the raw material of the second contact layer containing chitosan, polyurethane and the second antibacterial agent is sprayed on the one-way isolation film, then the liquid guide fibers are cut and are subjected to flat pressing to obtain the carrier with the second contact layer and the third contact layer; then, the first contact layer is pressed with the carrier with the second contact layer and the third contact layer to obtain the antibacterial sustained-release dressing.
[0027] Figure 1 Fig. 5 shows the liquid guide fiber electron microscope graph of the antibacterial sustained-release dressing provided in the embodiment; it can be observed that the structure of the liquid guide fiber provided in the embodiment in the microcosmic view is helpful to present a dense multi-cavity structure and is helpful to diffuse the liquid along the liquid guide fiber while flowing.
[0028] Figure 2 Fig. 6 shows the side cut surface structure schematic diagram of the antibacterial sustained-release dressing provided in the embodiment, which shows the liquid guide fiber distribution of the antibacterial sustained-release dressing provided in the embodiment on a cut surface, as shown in Figure 2 When the carrier 100 is located at the uppermost position, a part of the liquid guide fibers 240 prepared in the first liquid guide fiber implantation pass through the third contact layer 230 and the second contact layer 220 from the lower surface of the carrier 100, are pressed flat and embedded in the lower surface of the second contact layer 220, and are in contact with the upper surface of the first contact layer 210; a part of the liquid guide fibers 240 prepared in the second liquid guide fiber implantation extend downward from the inside of the third contact layer 230, pass out of the third contact layer 230 and pass through the second contact layer 220, and then are pressed flat and embedded in the lower surface of the second contact layer 220, and are in contact with the upper surface of the first contact layer 210.
[0029] Embodiment 2 The embodiment provides an antibacterial sustained-release dressing, and specifically comprises: a carrier and a contact body, the carrier is used for fixing the contact body on the skin surface and enabling the contact body to be in direct contact with the human body; The contact body comprises, from close to far, a first contact layer, a second contact layer and a third contact layer which are in contact with the human body; The raw material of the first contact layer comprises: microporous contact material, hemostatic agent and first antibacterial agent, the microporous contact material is non-woven fabric, the hemostatic agent is thrombin, and the first antibacterial agent is copper oxide and zinc oxide with a mass ratio of 1:1; The raw material of the second contact layer comprises: chitosan, polyurethane, second antibacterial agent and one-way isolation film, wherein the raw material of the second antibacterial agent is cellulose derivative and a second antibacterial core component, the second antibacterial core component is tea polyphenol, lysozyme and streptococcal nuclease; and the cellulose derivative is specifically carboxymethyl cellulose; The specific preparation method of the second antibacterial agent is as follows: carboxymethyl cellulose is dissolved in an aqueous solution, polyvinyl alcohol is added and uniformly mixed at 75 DEG C, then the second antibacterial core component is added, and after uniform mixing, heating is performed for 25 min; after heating is completed, cooling is performed, 3 min of cooling time is maintained for every 1 DEG C, and precipitation is performed, to obtain the second antibacterial agent.
[0030] The raw material of the third contact layer comprises: liquid absorbent, coagulant and third antibacterial agent, wherein the liquid absorbent is sodium carboxymethyl cellulose, the coagulant is hemagglutinin, and the third antibacterial agent is staphylococcus nuclease and lactic acid bacteria lysozyme; The antibacterial sustained-release dressing further comprises liquid guide fibers with a raw material of polycaprolactone, and the preparation method is: polycaprolactone is grafted with maleic anhydride and subjected to carboxylation treatment, and then electrospinning is performed; in the antibacterial sustained-release dressing provided in the embodiment, the liquid guide fibers pass through the second contact layer and are embedded in the third contact layer.
[0031] Secondly, the embodiment further provides a specific preparation method of the antibacterial sustained-release dressing, and the steps are as follows: The carrier is prepared, the carrier is subjected to glue coating to obtain a carrier with a glue coating surface; the first liquid guide fiber implantation is performed, the liquid guide fibers are adhered to the glue coating surface; then the first spraying of the third contact layer is performed, after the first spraying is completed, the second liquid guide fiber implantation is performed, then the second spraying of the third contact layer is performed; after the second spraying is completed, the one-way isolation film is sprayed, after the spraying of the one-way isolation film is completed, the raw material of the second contact layer containing chitosan, polyurethane and the second antibacterial agent is sprayed on the one-way isolation film, then the liquid guide fibers are cut and are subjected to flat pressing, to obtain the carrier with the second contact layer and the third contact layer; then the first contact layer is pressed with the carrier with the second contact layer and the third contact layer, to obtain the antibacterial sustained-release dressing.
[0032] Comparative Example 1 Firstly, the present comparative example provides an antibacterial sustained-release dressing, specifically comprising: a carrier and a contact body, the carrier is used to fix the contact body on the skin surface and make the contact body directly contact with the human body; The contact body includes, from near to far, a first contact layer, a second contact layer and a third contact layer in contact with the human body; The raw material of the first contact layer includes: a microporous contact material, a hemostatic agent and a first antibacterial agent, the microporous contact material is a non-woven fabric, the hemostatic agent is thrombin, and the first antibacterial agent is copper oxide and zinc oxide with a mass ratio of 1:1; The raw material of the second contact layer includes: chitosan, polyurethane, a second antibacterial agent and a one-way isolation film, wherein the raw material of the second antibacterial agent is a cellulose derivative and a second antibacterial core component, the second antibacterial core component is tea polyphenol, lysozyme and streptococcal nisin; the cellulose derivative is specifically carboxymethyl cellulose; The specific preparation method of the second antibacterial agent is: carboxymethyl cellulose is dissolved in an aqueous solution, polyvinyl alcohol is added and mixed uniformly at 75 ℃, then the second antibacterial core component is added, and after mixing, heating is carried out for 25 min; after the heating is completed, cooling is carried out, and every 1 ℃ cooling time lasts for 3 min, and then precipitation is carried out, to obtain the second antibacterial agent.
[0033] The raw material of the third contact layer includes: a liquid absorbent, a coagulant and a third antibacterial agent, wherein the liquid absorbent is sodium carboxymethyl cellulose, the coagulant is hemagglutinin, and the third antibacterial agent is staphylococcal enzyme and lactic acid bacterial lysozyme; Secondly, the present comparative example also provides a specific preparation method of the above antibacterial sustained-release dressing, the steps are as follows: Prepare the carrier, glue the carrier, and obtain the carrier with a glued surface; perform the first spraying of the third contact layer, perform the spraying of the one-way isolation film, after the spraying of the one-way isolation film is completed, spray the raw material of the second contact layer containing chitosan, polyurethane and the second antibacterial agent on the one-way isolation film, and perform flat pressing to obtain the carrier with the second contact layer and the third contact layer; then, the first contact layer is pressed with the carrier with the second contact layer and the third contact layer to obtain the antibacterial sustained-release dressing.
[0034] Comparative Example 2 Firstly, the present comparative example provides an antibacterial sustained-release dressing, specifically comprising: a carrier and a contact body, the carrier is used to fix the contact body on the skin surface and make the contact body directly contact with the human body; The contact body includes, from near to far, a first contact layer, a second contact layer and a third contact layer in contact with the human body; The raw material of the first contact layer includes: a microporous contact material, a hemostatic agent and a first antibacterial agent, the microporous contact material is a non-woven fabric, the hemostatic agent is thrombin, and the first antibacterial agent is copper oxide and zinc oxide with a mass ratio of 1:1; The raw material of the second contact layer comprises polyurethane, a second antibacterial agent and a unidirectional isolation film, wherein the raw material of the second antibacterial agent is a cellulose derivative and a second antibacterial core component, and the second antibacterial core component is tea polyphenol, lysozyme and streptococcal nuclease; the cellulose derivative is carboxymethyl cellulose in particular; The specific preparation method of the second antibacterial agent is as follows: carboxymethyl cellulose is dissolved in an aqueous solution, polyvinyl alcohol is added and mixed uniformly at 75 ℃, then the second antibacterial core component is added, and after mixing, heating is performed for 25 min; after the heating is completed, cooling is performed, with a cooling time of 3 min per 1 ℃, and precipitation is performed, to obtain the second antibacterial agent.
[0035] The raw material of the third contact layer comprises a liquid absorbent, a coagulant and a third antibacterial agent, wherein the liquid absorbent is sodium carboxymethyl cellulose, the coagulant is hemagglutinin, and the third antibacterial agent is staphylococcal nuclease and lactic acid bacterial nuclease; The antibacterial sustained-release dressing in the present comparative example further comprises a liquid guiding fiber with a raw material of polylactic acid, and the preparation method is as follows: polylactic acid is grafted with maleic anhydride and subjected to carboxylation treatment, and then electrospinning is performed; in the antibacterial sustained-release dressing provided by the present comparative example, the liquid guiding fiber penetrates through the second contact layer and is embedded in the third contact layer.
[0036] Secondly, the present comparative example further provides a specific preparation method of the above antibacterial sustained-release dressing, and the steps are as follows: A carrier is prepared, the carrier is coated to obtain a carrier with a coated surface; a first liquid guiding fiber implantation is performed to bond the liquid guiding fiber to the coated surface; then a first spraying of the third contact layer is performed, after the first spraying is completed, a second liquid guiding fiber implantation is performed, and then a second spraying of the third contact layer is performed; after the second spraying is completed, a spraying of the unidirectional isolation film is performed, after the spraying of the unidirectional isolation film is completed, a raw material of the second contact layer containing chitosan, polyurethane and a second antibacterial agent is sprayed on the unidirectional isolation film, then the liquid guiding fiber is cut and subjected to flat pressing, to obtain a carrier carrying the second contact layer and the third contact layer; then the first contact layer is pressed with the carrier carrying the second contact layer and the third contact layer, to obtain the antibacterial sustained-release dressing.
[0037] Performance test: The antibacterial sustained-release dressings prepared in the above embodiment 1, embodiment 2, comparative example 1 and comparative example 2, respectively, are taken as samples for the following tests.
[0038] Blood absorption performance test: Each test sample was accurately weighed, recorded as m1, then the sample carrier was placed with the contact surface upward on a watch glass, rat blood was slowly added to the watch glass until rat blood remained on the watch glass and could not be absorbed by the sample surface, then the mass of each sample was weighed, recorded as m2, the water absorption rate was calculated according to the formula, and the results are shown in Table 1.
[0039] Water absorption rate (%) = ((m2-m1) / m1) x 100% Table 1
[0040] The photos of the sample of Example 1 before and after the blood absorption performance test are shown in Figure 3 and Figure 4 .
[0041] Spray hemostatic performance test A rat femoral artery was incised using a scalpel to form a "V" shaped gap to construct an active spray bleeding model, which was used to test the specific effect of each sample above when facing a larger flow of bleeding; the specific results are shown in Table 2.
[0042] Table 3
[0043] Continuous bleeding hemostatic performance test: A rat femoral artery was punctured using a 30G needle to form a circular gap to construct a continuous bleeding model, which was used to test the specific effect of each sample above when facing a smaller flow of bleeding; the specific results are shown in Table 3.
[0044] Table 3
[0045] Wound healing test: The same batch of experimental mice were taken, after shaving the back of the mice using an electric shaver, a wound with a diameter of about 6 mm was caused on each mouse. Each mouse was covered with a sample, and the last group was a blank control group (cotton gauze without adding antibacterial sustained-release dressing). The wound surface of the mice was observed morphologically at 0, 3, 6, 9 days after injury, and the mouse wound photos in the wound healing experiment are shown in Figure 5 . The wound healing rate was calculated, and the specific data are shown in Table 4.
[0046] Table 4
[0047] While the application has been described in connection with very specific embodiments, it will be understood that the application is not limited to any of these details or embodiments or any special embodiment, but intends to cover all alternatives, modifications and equivalents as can be included within the scope of the application as defined by the following claims when interpreted in accordance with the principles of patent law. Furthermore, the above description is intended to provide a description of the application and is not intended to provide a complete description of the prior art.
Claims
1. An antimicrobial sustained release dressing, characterized in that, include: The carrier and the contact body; The contact body comprises a first contact layer, a second contact layer, and a third contact layer in sequence; The raw materials for the first contact layer include: microporous contact material, hemostatic agent, and first antibacterial agent; The raw materials for the second contact layer include: chitosan, polyurethane, a second antibacterial agent, and a one-way barrier membrane; The raw materials for the third contact layer include: liquid absorbent, coagulant and third antibacterial agent; The contact body also includes liquid guiding fibers that pass through the second contact layer and are embedded in the third contact layer.
2. An antimicrobial sustained-release dressing according to claim 1, characterized in that, The raw materials for the liquid-guided fiber include: polylactic acid, polybutylene succinate, and polycaprolactone.
3. An antimicrobial sustained-release dressing according to claim 1, characterized in that, The raw materials for the first antibacterial agent include at least one of titanium dioxide, copper oxide, zinc carbonate, zinc oxide, and zinc sulfate.
4. An antimicrobial sustained-release dressing according to claim 1, characterized in that, The raw materials of the second antibacterial agent include cellulose derivatives and a second antibacterial core component, which includes at least one of tea polyphenols, lysozyme, and nisin.
5. An antimicrobial sustained-release dressing according to claim 4, characterized in that The preparation method of the second antibacterial agent includes the following steps: dissolving the cellulose derivative in water, adding polyvinyl alcohol and mixing at 75 ℃-85 ℃, then adding the second antibacterial core component, mixing and heating for 20 min-40 min; after heating is completed, cooling is performed at a cooling rate of 0.15 ℃ / min-0.3 ℃ / min to precipitate and obtain the second antibacterial agent.
6. An antimicrobial sustained-release dressing according to claim 1, characterized in that, The raw materials for the third antibacterial agent include lysostaphylococcal enzyme and lactic acid bacteria lysozyme.
7. An antimicrobial sustained-release dressing according to claim 1, characterized in that, The microporous contact material includes microporous nonwoven fabric.
8. A method of preparing an antibacterial sustained-release dressing according to any one of claims 1 to 7, characterized in that, The procedure includes the following steps: preparing the carrier, applying adhesive to the carrier to obtain a carrier with an adhesive surface; performing a first liquid-guided fiber implantation, bonding the liquid-guided fiber to the adhesive surface; subsequently performing a first spraying of the third contact layer, and after completing the first spraying, performing a second liquid-guided fiber implantation, followed by a second spraying of the third contact layer; after completing the second spraying, spraying the unidirectional isolation membrane, and after completing the unidirectional isolation membrane spraying, spraying a mixture containing chitosan, polyurethane, and the second antibacterial agent onto the unidirectional isolation membrane, and then cutting the liquid-guided fiber to obtain the carrier carrying the second contact layer and the third contact layer; subsequently, pressing the first contact layer and the second contact layer together to obtain the antibacterial sustained-release dressing.
9. A method of making an antimicrobial sustained release dressing according to claim 8, wherein, The preparation method of the first contact layer includes the following steps: dissolving the hemostatic agent and the first antibacterial agent in a solvent to prepare a first contact layer loading solution, and immersing the microporous contact material in the first contact layer loading solution.
10. A method of making an antimicrobial sustained release dressing according to claim 8, wherein, When the liquid guiding fiber is cut, the exposed length of the liquid guiding fiber is 0.5 mm to 2 mm.