Anti-aging composition as well as preparation method and application thereof

Abstract

The invention provides an anti-aging composition as well as a preparation method and application thereof, and belongs to the technical field of cosmetics. The composition comprises Inca inchi oil, sandalwood seed oil, Morocco nut oil, olive fruit oil, a rosemary extract, vitamin E, Pinus densiflora leaf oil, valerian oil, astaxanthin and palmitoyl pentapeptide-4. According to the invention, a high-permeability matrix is constructed by utilizing scientific proportioning of a plurality of natural vegetable oils. The Pinus densiflora leaf oil, valerian oil and palmitoyl pentapeptide-4 have a synergistic effect, collagen synthesis is effectively promoted, and neuroinflammatory skin conditions are inhibited, so that aging caused by pressure is effectively resisted. And in combination with a specific anti-oxidation system, the technical problem that highly unsaturated grease is easy to oxidize and deteriorate is solved. The composition disclosed by the invention has excellent effects of relieving, repairing, resisting wrinkles and tightening; the essential oil is stable in property, comfortable in skin feeling and suitable for facial essential oil and other skin care products.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and more particularly to an anti-aging composition, its preparation method, and its uses. Background Technology

[0002] Skin aging is a complex biological process, mainly manifested as dry skin, weakened barrier function, wrinkles caused by collagen loss, and pigmentation. To combat skin aging, existing cosmetic technologies mainly employ two major technical approaches: one is to supplement bioactive peptides to stimulate collagen regeneration, and the other is to utilize natural plant oils to repair the skin's lipid barrier.

[0003] Palmitoyl pentapeptide-4 (PPP-4) is a classic signal peptide that mimics the degradation products of the extracellular matrix, thereby stimulating fibroblasts to synthesize type I collagen and hyaluronic acid, and is widely used in anti-aging products. However, in current technologies, using PPP-4 alone often has certain limitations. Studies have shown that modern people generally face problems such as high mental stress and poor sleep quality, leading to the skin being in a state of "micro-inflammation" or "stress-induced aging." In this microenvironment with high cortisol levels or active inflammatory factors, skin cell activity is inhibited, and even with peptide supplementation, collagen synthesis efficiency is difficult to achieve ideal levels. Currently, most anti-aging products focus on physically supplementing collagen, while neglecting the regulation of the skin's neuro-emotional state and microenvironment, resulting in the deep layers of the skin being in a state of "fatigue," thus significantly reducing the anti-aging effect.

[0004] On the other hand, plant oils (such as Sacha Inchi oil and sandalwood seed oil) are rich in unsaturated fatty acids, making them excellent emollients and penetration enhancers. However, high concentrations of unsaturated fatty acids are highly susceptible to lipid peroxidation, which not only causes product discoloration and off-odors but also exacerbates oxidative stress on the skin, accelerating aging. Currently, oil-based anti-aging products on the market either reduce the content of highly effective unsaturated fatty acids for stability, thus sacrificing repair effects; or rely on large amounts of synthetic antioxidants, increasing the risk of skin sensitization. In addition, the compatibility and transdermal absorption rate of single plant oils with water-soluble or amphiphilic active peptides are also technical challenges in formulation design.

[0005] Therefore, how to develop a composite anti-aging composition that can regulate skin stress to enhance cell vitality, solve the problem of highly active lipid oxidation stability, and achieve efficient collagen regeneration is an urgent problem to be solved in this field. Summary of the Invention

[0006] The purpose of this invention is to address the above-mentioned shortcomings of the prior art by providing an anti-aging composition, its preparation method, and its uses. This composition constructs a biomimetic sebum membrane system through a specific ratio of plant oils, and combines plant essential oils with nerve-soothing effects with highly effective anti-aging active ingredients to achieve a triple synergistic anti-aging effect of soothing and stress reduction, barrier repair, and firming and wrinkle reduction.

[0007] To achieve the above objectives, the present invention adopts the following technical solution: The first objective of this invention is to provide an anti-skin aging composition, which, by weight fraction, is made of the following components: 10-60 parts of Inca Inchi oil, 10-60 parts of sandalwood seed oil, 10-60 parts of argan oil, 1-20 parts of olive fruit oil, 0.1-1 parts of rosemary extract, 0.1-1 parts of vitamin E, 0.1-1 parts of European red pine leaf oil, 0.1-1 parts of valerian essential oil, 0.01-0.1 parts of astaxanthin, and 0.1-1 parts of palmitoyl pentapeptide-4.

[0008] Further, by weight fraction, it is made from the following components: 20-40 parts Inca Inchi oil, 20-40 parts Sandalwood seed oil, 15-35 parts Argan Oil, 5-15 parts Olive Fruit Oil, 0.2-0.8 parts Rosemary Extract, 0.3-0.8 parts Vitamin E, 0.2-0.6 parts European Red Pine Leaf Oil, 0.2-0.6 parts Valerian Essential Oil, 0.02-0.08 parts Astaxanthin, and 0.3-0.8 parts Palmitoyl Pentapeptide-4.

[0009] Furthermore, the mass ratio of the European red pine leaf oil to the valerian essential oil is 1:1 to 2:1.

[0010] Furthermore, the mass ratio of the Inca Inchi oil to the sandalwood seed oil is 1:1.5 to 1.5:1; the total mass of the Inca Inchi oil, sandalwood seed oil and argan oil in the composition accounts for more than 70% of the total mass of the composition.

[0011] Furthermore, the palmitoyl pentapeptide-4 is an oil-soluble modified palmitoyl pentapeptide-4 or a palmitoyl pentapeptide-4 premix dispersed in an oily matrix; the astaxanthin is selected from Haematococcus pluvialis extract.

[0012] Furthermore, the Inca Inchi oil, sandalwood seed oil, argan oil, and olive fruit oil mentioned are all cold-pressed virgin vegetable oils.

[0013] A second objective of this invention is to provide a method for preparing the above-mentioned anti-aging composition, comprising the following steps: S1. Mix Inca Inchi oil, sandalwood seed oil, argan oil and olive fruit oil according to the formula ratio, stir well to obtain a mixed oil phase; S2. Add rosemary extract and vitamin E to the mixed oil phase obtained in step S1, and stir to dissolve at 30~45℃; S3. When the temperature of the mixture obtained in step S2 drops below 35°C, palmitoyl pentapeptide-4, astaxanthin, European red pine leaf oil and valerian essential oil are added in sequence; after stirring evenly, the mixture is allowed to stand to remove bubbles and then filtered to obtain the anti-aging composition.

[0014] Furthermore, the palmitoyl pentapeptide-4 and astaxanthin were added under light-protected conditions, and the stirring time was controlled at 15-30 minutes.

[0015] Furthermore, the products include cosmetics and skincare products.

[0016] A third object of the present invention is to provide a cosmetic formulation comprising the anti-aging composition, wherein the dosage form of the cosmetic formulation comprises an essential oil, a massage oil, an oil capsule, an anhydrous ointment, or a water-in-oil emulsion.

[0017] A fourth objective of the present invention is to provide the above-described anti-aging composition for use in cosmetics having the effects of soothing nerves, repairing the skin barrier, firming and anti-wrinkle, or anti-aging the skin.

[0018] Compared with the prior art, the beneficial effects of the present invention are: (1) The anti-aging composition provided by this invention creatively combines European red pine leaf oil, valerian essential oil and palmitoyl pentapeptide-4. Spikeberry and valerian contain sesquiterpenoids, which have significant sedative, calming and nervous system regulating effects, and can effectively relieve the oxidative stress response of the skin caused by external stimuli, creating a stable microenvironment for the skin. On this basis, palmitoyl pentapeptide-4, as a signal peptide, can more efficiently identify the damaged dermis and stimulate the synthesis of collagen and elastin. Through the synergistic mechanism of first soothing and then repairing, it effectively solves the difficult problem of anti-aging for sensitive skin, significantly improves the firmness and elasticity of the skin, and relieves discomfort such as redness and stinging.

[0019] (2) This invention employs a base oil system composed of Inca Inchi oil (rich in α-linolenic acid), sandalwood seed oil (containing santalol), argan oil, and olive oil. Inca Inchi oil is rich in unsaturated fatty acids, possessing excellent skin affinity and permeability, promoting penetration and opening skin channels; while sandalwood seed oil promotes microcirculation. Utilizing the differences in the carbon chain length and unsaturation of fatty acids in different oils, a "gradient penetration" system is formed. This composite oil phase simulates the structure of the human sebum membrane, effectively encapsulating and delivering active ingredients such as palmitoyl pentapeptide-4 and astaxanthin to the dermis, thus solving the problem of peptide drugs being difficult to absorb transdermally. This improves the transdermal absorption rate of active ingredients, enhances the product's barrier repair and wrinkle-reducing effects, and avoids the potential damage to the skin barrier caused by traditional emulsifiers, achieving "skin nourishment with oil."

[0020] (3) This invention employs a multi-antioxidant system composed of rosemary extract, vitamin E, and astaxanthin, which solves the problem of easy inactivation of photosensitive components. Although astaxanthin has strong antioxidant capacity, it is easily oxidized. This invention utilizes rosemary extract and vitamin E to construct an antioxidant network: vitamin E acts as the first line of defense to quench free radicals, while rosemary extract can protect oils from rancidity and synergistically stabilize the molecular structure of astaxanthin, preventing its decomposition upon exposure to light.

[0021] (4) The formula provided by this invention is an anhydrous all-oil system with a high total content of each plant oil component. Utilizing the excellent occlusive and film-forming properties of oils, a breathable protective film is formed on the skin surface to lock in moisture. At the same time, the phytosterols rich in argan oil and sandalwood seed oil can repair the lipids of the stratum corneum. While reducing wrinkles, it significantly improves dry and rough skin, enhances the skin barrier function, and restores the skin to a soft, delicate, and radiant state. Detailed Implementation

[0022] The present invention will be further described below with reference to embodiments. It should be understood that the embodiments are only used to further illustrate and explain the present invention, and are not intended to limit the present invention.

[0023] Unless otherwise defined, technical and scientific terms used in this specification have the same meaning as commonly understood by one of ordinary skill in the art. While similar or identical methods and materials may be applied in experimental or practical applications, materials and methods are described herein. In case of conflict, the definitions included herein shall prevail. Furthermore, materials, methods, and examples are for illustrative purposes only and are not intended to be limiting. The invention is further described below with reference to specific embodiments, but is not intended to limit the scope of the invention.

[0024] The raw materials used in the embodiments of this invention are all commercially available cosmetic grade raw materials.

[0025] Inca Inchi oil, sandalwood seed oil, argan oil, and olive fruit oil: all are cold-pressed virgin grade, not refined at high temperatures, retaining natural unsaponifiables.

[0026] Inca Inchi oil: The color is golden yellow to amber, with an alpha-linolenic acid content of not less than 45% and a linoleic acid content of not less than 35%.

[0027] Sea buckthorn seed oil: contains specific long-chain fatty acids, of which sea buckthorn acid content is not less than 8%.

[0028] Argan oil: rich in squalene and phytosterols.

[0029] Palmitoyl pentapeptide-4: It uses an oil-soluble modified premix with an active ingredient content of 1000 ppm and a matrix of caprylic / capric triglycerides.

[0030] Astaxanthin: Derived from Haematococcus pluvialis extract, it is an oil-soluble liquid with an astaxanthin content of 5%.

[0031] The anti-aging composition provided by this invention is formulated as follows: by weight fraction, it is made from the following components: 10-60 parts of Sacha Inchi oil, 10-60 parts of sandalwood seed oil, 10-60 parts of argan oil, 1-20 parts of olive fruit oil, 0.1-1 parts of rosemary extract, 0.1-1 parts of vitamin E, 0.1-1 parts of European red pine leaf oil, 0.1-1 parts of valerian essential oil, 0.01-0.1 parts of astaxanthin, and 0.1-1 parts of palmitoyl pentapeptide-4.

[0032] Preferably, it is made from the following components by weight fraction: 20-40 parts of Inca Inchi oil, 20-40 parts of sandalwood seed oil, 15-35 parts of argan oil, 5-15 parts of olive fruit oil, 0.2-0.8 parts of rosemary extract, 0.3-0.8 parts of vitamin E, 0.2-0.6 parts of European red pine leaf oil, 0.2-0.6 parts of valerian essential oil, 0.02-0.08 parts of astaxanthin, and 0.3-0.8 parts of palmitoyl pentapeptide-4.

[0033] The method for preparing the anti-aging composition provided by this invention is as follows: Preparation of the base oil phase: Weigh out Inca Inchi oil, sandalwood seed oil, argan oil, and olive fruit oil according to the preset ratio and add them to a stainless steel mixing jar. Stir with a paddle mixer (100-200 rpm) for 5 minutes, or until the mixture is clear and transparent.

[0034] Antioxidant system construction: Heat the mixed oil phase to 35-40°C. Add rosemary extract and vitamin E, and maintain this temperature while stirring for 10 minutes until completely dissolved. Temperature control in this step aims to promote the dissolution of antioxidants while preventing oil oxidation.

[0035] Adding active ingredients and flavorings: Stop heating and start cooling water circulation. When the mixture temperature drops below 30°C, add palmitoyl pentapeptide-4, astaxanthin, European red pine leaf oil, and valerian oil sequentially under this low-temperature environment. This step must be carried out in the dark. Adjust the stirring speed to 150 rpm and control the stirring time to 20 minutes to prevent the heat-sensitive astaxanthin from fading and the peptides from becoming inactive.

[0036] Homogenization and filling: Stop stirring, let stand for 24 hours to remove air bubbles, filter with a 0.45μm microporous membrane to remove impurities, then fill with nitrogen and seal.

[0037] In some implementations, natural plant oils with similar fatty acid composition or skin feel can also be used in the preparation of the base oil phase. For example, Sacha Inchi oil can be replaced by oils rich in α-linolenic acid, such as flaxseed oil and perilla seed oil; sandalwood seed oil can be replaced by oils with long-chain fatty acid or wax ester structure and good film-forming properties, such as jojoba oil and meadowfoam seed oil; and olive fruit oil can be replaced by sweet almond oil, avocado oil, sunflower seed oil, or camellia seed oil.

[0038] In addition, to adjust the skin feel, appropriate amounts of cosmetic-grade synthetic oils, such as caprylic / capric triglycerides (GTCC), squalane (of plant or synthetic origin), and isononyl isononanoate, can be added to the base oil phase, as long as it does not affect the overall permeability.

[0039] In some implementations, specific blends of spikenard and valerian essential oils are used, adhering to the concept of "soothing nerves and delaying aging through the olfactory pathway." These essential oils can be replaced with other plant essential oils with similar calming and soothing effects. For example: European red pine leaf oil can be substituted with vetiver, patchouli, or cedarwood essential oil; Valerian essential oil can be replaced with lavender, Roman chamomile, ylang-ylang, petitgrain, or marjoram essential oils; These essential oils can be used alone or mixed in any proportion to achieve similar aromatherapy effects.

[0040] In some implementations, palmitoyl pentapeptide-4, as a collagen promoter, can be replaced by other peptides with anti-wrinkle and firming effects, such as acetyl hexapeptide-8, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, and copper peptide; it can also be replaced by oil-soluble anti-aging ingredients, such as retinol (A-ol) and its derivatives, as well as propylene glycol (hydroxypropyl tetrahydropyranotriol). Astaxanthin, a potent antioxidant, can be replaced by coenzyme Q10, fullerene, idebenone, oil-soluble vitamin C derivatives (such as ascorbate tetraisopalmitate VC-IP), or lycopene. Rosemary extract can be replaced by oil-soluble antioxidants, such as tea polyphenol palmitate, butylated hydroxyanisole (BHA), and butylated hydroxytoluene (BHT).

[0041] The total content of European red pine leaf oil and valerian oil is 0.1% to 2.0%, and their mass ratio can be extended from the preferred ratio of 2:1 to 1:5 to 5:1.

[0042] The addition amount of palmitoyl pentapeptide-4 (premix) is 0.1% to 5.0%, depending on the cost and efficacy positioning of the target product.

[0043] The amount of astaxanthin added is 0.01% to 0.5%.

[0044] In some implementations, the heating temperature during the antioxidant system construction stage can be increased to 30°C to 55°C; the temperature for adding the active ingredient can be reduced to below 35°C (as long as it is below the inactivation temperature of the active ingredient); the time can be adjusted to 5 to 60 minutes according to the shear efficiency of the production equipment; and a filter membrane or filter screen of 0.22 μm to 5.0 μm can be selected according to the viscosity of the material.

[0045] The cosmetic formulation provided by this invention can be a homogeneous oil-based system (fragrance oil), and can also be applied to other oil-containing dosage forms, such as: Water-in-oil (W / O) or oil-in-water (O / W) emulsions / creams: prepared by adding an emulsifier and an aqueous phase (as an oil phase) to the composition of the present invention; Two- or multi-layer formulation: Prepared as a "water-oil two-phase" essence, which separates into layers after standing. Shake well before use. Capsules: The composition of the present invention is encapsulated in disposable soft capsules; Body care: In addition to facial care, this composition can also be adjusted in proportion for use in body massage oils, hair oils or bath oils.

[0046] The embodiments of the present invention have achieved some positive results during the research and development or use process, and have indeed great advantages compared with the prior art. The following content describes them in conjunction with the data, charts and other information of the experimental process.

[0047] The Inca Inchi seed oil used in the embodiments provided by this invention was provided by Guangzhou Shengjia Biotechnology Co., Ltd., the sandalwood seed oil by Guangzhou Zhongyi Suyuan Biotechnology Co., Ltd., the argan oil and olive oil by TEXTRONPLIMON, SLU, the rosemary extract by Draco Natural Products Inc., the vitamin E by DSM Nutrition Products Ltd., the European red pine leaf oil by SARL Distillerie Raoul Duffez, the valerian essential oil by Jiangxi Hanhong Biotechnology Co., Ltd., the astaxanthin by Xianting (Guangzhou) Trading Co., Ltd., and the palmitoyl pentapeptide-4 by Shaanxi Huikang Biotechnology Co., Ltd.

[0048] Example 1 This embodiment provides an anti-aging composition comprising the following ingredients by weight percentage: Sacha Inchi Oil: 30%; Sandalwood seed oil: 30%; Argan oil: 25%; Olive fruit oil: 12.6%; Rosemary extract: 0.5%; Vitamin E: 0.5%; European red pine leaf oil: 0.4%; Valerian essential oil: 0.2%; Astaxanthin: 0.05%; Palmitoyl pentapeptide-4: 0.75%.

[0049] The preparation method is as follows: (1) Weigh out Inca Inchi oil, sandalwood seed oil, argan oil and olive fruit oil according to the preset ratio and add them to a stainless steel mixing tank. Stir with a paddle mixer at 100 for 5 minutes until the mixture is clear and transparent to obtain a mixed oil phase.

[0050] (2) Heat the mixed oil phase to 35-40°C. Add rosemary extract and vitamin E, and stir at this temperature for 10 minutes until completely dissolved. The temperature control in this step is intended to promote the dissolution of antioxidants while preventing oil oxidation.

[0051] (3) Stop heating and start cooling water circulation. When the temperature of the mixture drops below 30°C, add palmitoyl pentapeptide-4, astaxanthin, European red pine leaf oil and valerian oil in sequence under this low temperature environment. This step must be carried out in the dark. Adjust the stirring speed to 150 rpm and control the stirring time to 20 min to prevent the heat-sensitive astaxanthin from fading and the peptides from becoming inactive.

[0052] Homogenization and filling: Stop stirring, let stand for 24 hours to remove air bubbles, filter with a 0.45μm microporous membrane to remove impurities, then fill with nitrogen and seal.

[0053] Example 2 This embodiment provides an anti-aging composition comprising the following ingredients by weight percentage: Sacha Inchi Oil: 25%; Sandalwood seed oil: 35%; Argan oil: 30%; Olive fruit oil: 7.8%; Rosemary extract: 0.3%; Vitamin E: 0.8%; European red pine leaf oil: 0.3%; Valerian essential oil: 0.25%; Astaxanthin: 0.05%; Palmitoyl pentapeptide-4: 0.5%.

[0054] The preparation method is the same as in Example 1.

[0055] Example 3 This embodiment provides an anti-aging composition, the composition of which (by weight percentage) is as follows: Sacha Inchi Oil: 40%; Sandalwood seed oil: 25%; Argan oil: 20%; Olive fruit oil: 12.2%; Rosemary extract: 0.5%; Vitamin E: 0.5%; European red pine leaf oil: 0.5%; Valerian essential oil: 0.3%; Astaxanthin: 0.1%; Palmitoyl pentapeptide-4: 0.9%.

[0056] The preparation method is the same as in Example 1.

[0057] Comparative Example 1 Olive fruit oil was used instead of Inca Inchi oil, sandalwood seed oil, and argan oil; the remaining active ingredients and their contents were the same as in Example 1.

[0058] Comparative Example 2 Rosemary extract was removed, retaining only vitamin E; the missing mass fraction was made up with olive fruit oil. Other ingredients were the same as in Example 1.

[0059] Comparative Example 3 Remove European red pine leaf oil and valerian essential oil; replenish the remaining ingredients with an equal amount of olive fruit oil. Other ingredients are the same as in Example 1.

[0060] Comparative Example 4 Adjust the essential oil ratio to use 0.1% European red pine leaf oil and 0.5% valerian essential oil. Other ingredients are the same as in Example 1.

[0061] Comparative Example 5 Astaxanthin and palmitoyl pentapeptide-4 were removed, and the missing mass fraction was made up with olive fruit oil. Other ingredients were the same as in Example 1.

[0062] Performance testing and effect evaluation 1. Transdermal absorption test of palmitoyl pentapeptide-4 Franz diffusion cell was used, with ex vivo porcine skin as a skin model. Equal volumes of samples from Examples 1-3 and Comparative Example 1 were added to the supply cell, while physiological saline was used as the receiving cell. Permeation tests were conducted at a constant temperature of 32°C, and the cumulative permeation amount of palmitoyl pentapeptide-4 in the receiving cell and skin layer was measured after 24 hours. The cumulative permeation amount of Example 1 after 24 hours was 16.45 μg / cm³. 2 Example 2 showed a cumulative permeation of 12.73 μg / cm³ after 24 hours. 2 Example 3 showed a cumulative permeation of 15.28 μg / cm³ after 24 hours. 2 The cumulative permeability of Comparative Example 1 after 24 hours was 4.12 μg / cm³. 2 This indicates that the gradient oil system composed of Inca Inchi oil, sandalwood seed oil, and argan oil can increase the transdermal absorption rate of peptide active sites by about 3 to 4 times compared with ordinary occlusive oils, significantly improving the bioavailability of palmitoyl pentapeptide-4.

[0063] 2. Astaxanthin stability test Samples from Examples 1-3 and Comparative Example 2 were placed in two transparent bottles and incubated at 45°C for 28 days. The astaxanthin content in the samples was determined using high-performance liquid chromatography (HPLC), and the retention rate was calculated. The astaxanthin retention rate for Example 1 on day 28 was 89.5%; for Example 2, it was 85.7%; for Example 3, it was 87.3%; and for Comparative Example 2, it was only 62.3%. This demonstrates that the specific ratio of rosemary extract and vitamin E used in this invention can significantly inhibit the oxidative decomposition of astaxanthin, solving the technical problem of astaxanthin's instability in oily systems.

[0064] 3. Human patch test Thirty volunteers with sensitive skin were selected. They were first subjected to mild chemical stimulation (sodium lauryl sulfate), followed by application of samples from Examples 1-3 and Comparative Example 3. The rate of decrease in erythema index was measured after 24 hours. A higher rate of decrease in erythema index indicated a better soothing and repairing effect. The erythema reduction rate was 45.6% in Example 1; 42.9% in Example 2; 44.2% in Example 3; and 22.5% in Comparative Example 3. This demonstrates that European red pine leaf oil and valerian essential oil have powerful soothing effects and inhibit inflammatory factors.

[0065] 4. Evaluation of human efficacy Sixty female volunteers aged 35-55 years with obvious signs of stress-induced aging (such as dull complexion and facial sagging due to lack of sleep) were randomly assigned to five groups of 12 each. They used the products from Examples 1-3 and Comparative Examples 1-4 once daily in the evening for 28 consecutive days. The test results are shown in Table 1, and the test indicators are as follows: Transdermal water loss (TEWL) improvement rate: tested using Tewameter TM300; the higher the value, the stronger the skin barrier repair ability.

[0066] Skin elasticity improvement rate: The change in the R2 parameter was evaluated using a Cutometer MPA580.

[0067] Cell viability assay (CCK-8 assay): Cells from different groups were seeded at a density of 5 × 10³ cells per well in 96-well plates, with 6 replicates per group. After 24 hours of culture, the old culture medium was discarded, and fresh culture medium containing different treatments was added, and the cells were cultured for another 48 hours. Two hours before the end of the culture period, 10 μL of CCK-8 solution was added to each well, and incubation continued. The absorbance (OD value) of each well was then measured at 450 nm using a microplate reader. Cell viability was calculated using the formula: Cell viability (%) = (OD value of experimental group - OD value of blank group) / (OD value of control group - OD value of blank group) × 100%. The OD value of the blank control group was 0.50 ± 0.03. Collagen production rate: Human skin fibroblasts were cultured, diluted sample extract was added, and collagen secretion was measured after 48 hours of culture, with the blank control group as 100% baseline. The collagen secretion level in the blank control group was 50±5 ng / mL.

[0068] Soothing and sleep-aiding effect rating (subjective): After use, volunteers rated their relaxation level and ease of falling asleep on a scale of 10; the higher the score, the better the effect.

[0069] Penetration and absorption score (subjective): assesses the speed of product absorption on the skin and the feeling of oiliness; 10 points indicates very fast absorption and a feeling of dryness, and 1 point indicates very poor absorption and a feeling of oiliness.

[0070] Table 1. Statistical table of human efficacy evaluation results.

[0071]

[0072] As shown in Table 1, simply supplementing with palmitoyl pentapeptide-4 limits the efficiency of collagen synthesis in skin under micro-inflammatory or stressful conditions. This invention significantly improves the efficiency of palmitoyl pentapeptide-4 in promoting collagen synthesis by inhibiting neurogenic inflammatory factors. Simultaneously, the gradient penetration system constructed from Inca Inchi oil, sandalwood seed oil, and argan oil is similar to a biomimetic sebum membrane, enabling the poorly water-soluble palmitoyl pentapeptide-4 to quickly cross the skin barrier, achieving deep anti-aging.

[0073] In summary, this invention, through a specific oil ratio and a combination of "soothing + anti-aging" active ingredients, not only solves the problems of poor skin feel and slow absorption associated with pure oil formulations, but also significantly improves the bioavailability of anti-aging ingredients by enhancing the skin's microenvironment. The anti-aging composition provided by this invention can be used to prepare cosmetics with effects such as soothing nerves, repairing the skin barrier, firming and anti-wrinkle, or anti-aging. Its dosage form can include essential oils, massage oils, oil capsules, anhydrous ointments, or water-in-oil emulsions.

[0074] For any points not covered above, existing technologies shall apply.

[0075] Although specific embodiments of the present invention have been described in detail by way of examples, those skilled in the art should understand that the above examples are for illustrative purposes only and are not intended to limit the scope of the invention. Those skilled in the art can make various modifications or additions to the described specific embodiments or use similar methods to replace them, without departing from the direction of the invention or exceeding the scope defined by the appended claims. Those skilled in the art should understand that any modifications, equivalent substitutions, improvements, etc., made to the above embodiments based on the technical essence of the present invention should be included within the protection scope of the present invention.

Claims

1. An anti-aging composition characterized in that, According to the weight fraction, it is made of: 10-60 parts of Inca oil, 10-60 parts of Tagua palm seed oil, 10-60 parts of argan oil, 1-20 parts of olive fruit oil, 0.1-1 part of rosemary extract, 0.1-1 part of vitamin E, 0.1-1 part of European red pine leaf oil, 0.1-1 part of valerian essential oil, 0.01-0.1 part of astaxanthin, and 0.1-1 part of palmitoyl pentapeptide-4.

2. Anti-aging composition according to claim 1, characterized in that, According to the weight fraction, it is made of: 20-40 parts of Inca oil, 20-40 parts of Tagua palm seed oil, 15-35 parts of argan oil, 5-15 parts of olive fruit oil, 0.2-0.8 parts of rosemary extract, 0.3-0.8 parts of vitamin E, 0.2-0.6 parts of European red pine leaf oil, 0.2-0.6 parts of valerian essential oil, 0.02-0.08 parts of astaxanthin, and 0.3-0.8 parts of palmitoyl pentapeptide-4.

3. Anti-aging composition according to claim 1, characterized in that, The mass ratio of the European red pine leaf oil to the valerian essential oil is 1:1~2:

1.

4. The anti-aging composition according to claim 1, characterized in that, The mass ratio of the Inca oil to the Tagua palm seed oil is 1:1.5~1.5:1; the total mass of the Inca oil, the Tagua palm seed oil and the argan oil in the composition accounts for more than 70% of the total mass of the composition.

5. The anti-aging composition according to claim 1, characterized in that, The palmitoyl pentapeptide-4 is an oil-soluble modified palmitoyl pentapeptide-4 or a palmitoyl pentapeptide-4 premix dispersed in an oily base; the astaxanthin is selected from a Haematococcus pluvialis extract.

6. The anti-aging composition according to claim 1, characterized in that, The Inca oil, the Tagua palm seed oil, the argan oil and the olive fruit oil are all cold-pressed plant oils.

7. The method of preparing an anti-aging composition according to any one of claims 1-6, wherein, The method comprises the following steps: S1, mixing the Inca oil, the Tagua palm seed oil, the argan oil and the olive fruit oil according to the formula proportion, stirring uniformly to obtain a mixed oil phase; S2, adding the rosemary extract and the vitamin E to the mixed oil phase obtained in step S1, stirring and dissolving at 30~45℃; S3, when the temperature of the mixture obtained in step S2 is reduced to below 35℃, sequentially adding the palmitoyl pentapeptide-4, the astaxanthin, the European red pine leaf oil and the valerian essential oil; after stirring uniformly, standing to remove bubbles, filtering to obtain the anti-aging composition.

8. The method of claim 7, wherein, The palmitoyl pentapeptide-4 and the astaxanthin are added under light-proof conditions, and the stirring time is controlled at 15~30min.

9. A cosmetic preparation comprising the anti-aging composition according to any one of claims 1 to 6, characterized in that, The dosage form of the cosmetic preparation comprises an essence oil, a massage oil, an oil capsule, an anhydrous ointment or a water-in-oil emulsion.

10. Use of the anti-aging composition of any one of claims 1-6 in the preparation of a cosmetic having the effects of soothing nerves, repairing skin barrier, tightening and anti-wrinkle, or anti-skin aging.

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