Traditional Chinese medicine composition and toothpaste for inhibiting helicobacter pylori
By combining extracts of green tea, honeysuckle, dandelion, and clove into toothpaste, the problem of unsatisfactory effects of existing antibacterial products is solved, providing a safe and effective traditional Chinese medicine toothpaste that inhibits Helicobacter pylori, thus meeting the demand for green and healthy products.
Patent Information
- Application Number
- CN202410870887.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-07-01
- Publication Date
- 2026-01-06
AI Technical Summary
Existing oral care products that inhibit Helicobacter pylori have unsatisfactory antibacterial effects, and most contain chemical additives, lacking green and healthy alternatives.
A traditional Chinese medicine toothpaste that inhibits Helicobacter pylori was prepared by using a combination of extracts from green tea, honeysuckle, dandelion, and clove as the main ingredients, along with excipients such as abrasives and thickeners.
It effectively inhibits the growth of Helicobacter pylori, protects oral hygiene, has safe and non-toxic components with no side effects, and has a fresh scent that is widely accepted.
Smart Images
Figure CN121265700A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of oral care product technology, specifically to a traditional Chinese medicine composition and toothpaste that inhibits Helicobacter pylori. Background Technology
[0002] Helicobacter pylori (HP) is a microaerophilic, Gram-negative bacterium that can colonize areas such as the oral cavity and gastric mucosa. It is an infectious pathogen and a major contributing factor to infectious halitosis, oral ulcers, chronic active gastritis, peptic ulcers, and gastric cancer. In 2017, the World Health Organization classified Helicobacter pylori as a Group 1 carcinogen.
[0003] As the entrance to the digestive tract, the presence of Helicobacter pylori in the oral cavity affects the efficacy of Helicobacter pylori eradication therapy. Maintaining good oral hygiene and preventing oral infections are increasingly becoming important measures for preventing Helicobacter pylori infection, halitosis, and stomach problems. Oral products that inhibit Helicobacter pylori can reduce the probability of Helicobacter pylori entering and colonizing the stomach, thus reducing its pathogenicity and improving the efficacy of eradication therapy. Although some products that inhibit Helicobacter pylori exist, such as mouthwash, toothpaste, and oral sprays, their antibacterial effects are not ideal.
[0004] Therefore, how to provide an oral product that can effectively inhibit Helicobacter pylori has become a problem that needs to be solved. Summary of the Invention
[0005] To address the aforementioned problems, this invention provides a traditional Chinese medicine composition and toothpaste that inhibit Helicobacter pylori. This composition has a good inhibitory effect on Helicobacter pylori, and its components are all plant extracts, making the raw materials green and healthy. When added to toothpaste, it not only effectively inhibits Helicobacter pylori but also has no side effects.
[0006] To achieve the above objectives, the specific technical solution adopted by the present invention is as follows:
[0007] A first aspect of the present invention is to provide a traditional Chinese medicine composition for inhibiting Helicobacter pylori, comprising green tea extract, honeysuckle extract, dandelion extract and clove extract.
[0008] The innovation of this invention lies in the discovery, through screening combinations of different plant extracts, that the combination of the above four plant extracts exhibits an inhibitory advantage against Helicobacter pylori, and at a relatively low cost.
[0009] A second aspect of the present invention is to provide the application of the above-mentioned traditional Chinese medicine composition in the preparation of oral products that inhibit Helicobacter pylori. Specifically, a toothpaste for inhibiting Helicobacter pylori is provided, comprising plant-based active ingredients and excipients, wherein the plant-based active ingredients are the traditional Chinese medicine composition for inhibiting Helicobacter pylori.
[0010] Furthermore, the excipients include abrasives, thickeners, humectants, foaming agents, cooling agents, preservatives, anti-caries agents, antioxidants, and antibacterial agents. The abrasive is silica, the thickener is xanthan gum, the humectant includes glycerol, propylene glycol, and sorbitol, the foaming agent is sodium lauryl sulfate, the cooling agent is menthol, the preservative is sodium benzoate, the anti-caries agent is sodium monofluorophosphate, the antioxidant is 2,6-di-tert-butyl-p-cresol (BHT), and the antibacterial agent is domiphen (which has inhibitory effects on Staphylococcus aureus, Escherichia coli, and Candida albicans).
[0011] Furthermore, the toothpaste comprises, by weight, the following components: 1-20 parts green tea extract, 1-20 parts honeysuckle extract, 1-20 parts dandelion extract, 1-20 parts clove extract, 100-1000 parts silica, 1-30 parts xanthan gum, 100-500 parts sorbitol, 100-500 parts propylene glycol, 100-500 parts glycerol, 10-40 parts sodium lauryl sulfate, 1-20 parts sodium benzoate, 1-40 parts menthol, 0-6 parts 2,6-di-tert-butyl-p-cresol, 1-30 parts sodium monofluorophosphate, and 1-10 parts domiphen.
[0012] Furthermore, the toothpaste that inhibits Helicobacter pylori is prepared by the following method:
[0013] (1) Add the thickener to deionized water and stir until well mixed;
[0014] (2) While stirring, add the plant active ingredients, moisturizer, foaming agent, cooling agent, preservative, anti-caries agent, antioxidant and antibacterial agent to the glue obtained in step (1) and stir evenly.
[0015] (3) Add the abrasive in small amounts several times, and mix well.
[0016] (4) Stop stirring and sterilize to obtain the oral paste product.
[0017] The present invention has the following beneficial effects:
[0018] This invention relates to a compound herbal toothpaste, which rationally combines extracts from four traditional Chinese medicines—green tea, honeysuckle, dandelion, and clove—to inhibit the growth of Helicobacter pylori, protect oral hygiene, and prevent Helicobacter pylori infection. The toothpaste's components are all made from green and healthy raw materials, with no toxic side effects. Its unique and sophisticated formula, simple manufacturing method, and refreshing scent make it easily acceptable to a wide range of people. Attached Figure Description
[0019] Figure 1 Experimental data from the 8-factor, 4-level orthogonal experiment in Example 1. Detailed Implementation
[0020] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.
[0021] Example 1
[0022] This embodiment provides a traditional Chinese medicine composition for inhibiting Helicobacter pylori, comprising green tea extract, honeysuckle extract, dandelion extract and clove extract.
[0023] The screening and determination method for this traditional Chinese medicine composition is as follows: Based on previous experiments, the MBC (minimum bactericidal concentration) of each extract was determined, and an 8-factor, 4-level orthogonal experiment was conducted at 2-4 times the value to determine the composition by comprehensively considering the antibacterial effect and cost. The specific process is as follows:
[0024] I. Raw Material Preparation
[0025] Prepare the following 6 plant extracts: green tea extract, honeysuckle extract, dandelion extract, dried plum extract, clove extract, and licorice extract.
[0026] The specific sources of the raw materials used in this embodiment are as follows: Green tea extract was purchased from Zhejiang Shengtong Biotechnology Co., Ltd., batch number 221210E; honeysuckle extract, dandelion extract, dried plum extract, clove extract, and licorice extract were all purchased from Baoji Xuhuang Biotechnology Co., Ltd., batch numbers 220801, 220701, 220901, 221001, and 221001, respectively.
[0027] II. Antibacterial Test
[0028] Prepare the raw materials according to Figure 1 The raw materials were mixed according to the combination method and the amount (parts by weight) of the additives, using deionized water as the solvent, to prepare 32 sets of experimental samples. The experimental samples were sterilized at 121℃ for 20 minutes. After sterilization, the samples were placed in a biosafety cabinet through a transfer window. 50 μL of Helicobacter pylori bacterial suspension was spread onto HP medium. Four 6 mm circular paper discs were evenly placed on the medium, and 10 μL of sample was dropped onto each disc for diffusion. After the operation, the medium was placed in an incubator for cultivation. The size of the inhibition zone on the medium was observed and measured; a larger inhibition zone indicated a better antibacterial effect.
[0029] Test results are as follows Figure 1 As shown, it can be seen that 6 groups have good antibacterial effects (inhibition zone diameter ≥10mm). After considering the cost, the extract combination of group 32 was selected as the composition of the traditional Chinese medicine composition of the present invention, which is composed of green tea extract, honeysuckle extract, dandelion extract and clove extract.
[0030] Among the identified traditional Chinese medicine compositions, the four extracts, in addition to exhibiting an anti-Helicobacter pylori effect when combined, also possess the following individual effects, making them superior toothpaste raw materials.
[0031] The green tea extract has the effects of clearing the head and eyes, relieving thirst, aiding digestion, resolving phlegm, promoting urination, and detoxifying. Clinically, it has a therapeutic effect on dental plaque and gingival bleeding.
[0032] The honeysuckle extract has the effects of clearing heat and detoxifying, reducing inflammation and swelling. Clinically, it has a therapeutic effect on oral ulcers, tooth decay, and gingivitis, and can also inhibit halitosis.
[0033] The dandelion extract has the effects of clearing heat and detoxifying, reducing swelling and dissipating nodules, and promoting diuresis and relieving strangury. Dandelion has antibacterial and anti-inflammatory effects and can treat oral ulcers.
[0034] The clove extract has the effects of warming the middle jiao and relieving nausea, tonifying the kidney and assisting yang, and has a therapeutic effect on toothache and oral ulcers in clinical practice.
[0035] Example 2
[0036] This invention provides a toothpaste that inhibits Helicobacter pylori, the composition of which is shown in the table below. This embodiment provides some specific examples of this toothpaste formulation (see groups 1-3 in the table below).
[0037]
[0038] Weigh the raw materials according to the dosage (parts by weight) in the table above, and prepare the toothpaste for groups 1-3 according to the following steps:
[0039] (1) Add the thickener to deionized water and stir until well mixed;
[0040] (2) While stirring, add the plant active ingredients, moisturizer, foaming agent, cooling agent, preservative, anti-caries agent, antioxidant and antibacterial agent to the glue obtained in step (1) and stir evenly.
[0041] (3) Add the abrasive in small amounts several times, and mix well.
[0042] (4) Stop stirring and sterilize to obtain the oral paste product.
[0043] Note: Friction-absorbing agents are highly absorbent, so they should be added last.
[0044] Toothpaste antibacterial test:
[0045] The toothpaste samples from groups 1-3 were processed as follows: A certain amount of toothpaste was weighed into a bottle, and an equal volume of deionized water was added to obtain a toothpaste suspension. The suspension was separated using a centrifuge, and the supernatant was used as the experimental sample. The experimental sample was sterilized at 121℃ for 20 minutes. After sterilization, the sample was transferred to a biosafety cabinet through a transfer window. An experimental bacterial suspension with a concentration of 1×10⁸ CFU / mL was prepared. In a sterile test tube for the detoxification test, 0.5 mL of Helicobacter pylori suspension was added, followed by 0.5 mL of 3% BSA solution. The mixture was mixed and allowed to stand at room temperature for 5 minutes. Then, 4.0 mL of the sample was injected into the suspension using a sterile pipette and quickly mixed. After the two components interacted, 0.5 mL of the Helicobacter pylori and sample mixture was added to 4.5 mL of 3% BSA solution, mixed, and allowed to stand for 10 minutes. 1.0 mL of the sample solution was then inoculated onto a culture medium, spreading evenly. Each sample solution was inoculated onto two petri dishes. All test samples were incubated in a 37°C incubator. The number of viable bacteria was determined and counted according to the viable bacteria culture counting method.
[0046] The results showed that the toothpastes in groups 1-3 all achieved an inhibition rate of 99.99% against Helicobacter pylori, demonstrating significant antibacterial effects.
[0047] This specific embodiment is merely an explanation of the present invention and is not intended to limit the present invention. Any changes made by those skilled in the art after reading the specification of the present invention, as long as they are within the scope of the claims of the present invention, will be protected by patent law.
Claims
1. A traditional Chinese medicine composition for inhibiting Helicobacter pylori, characterized in that, The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract.
2. A toothpaste for inhibiting Helicobacter pylori, characterized by comprising, The toothpaste comprises the plant effective component and adjuvants.
3. The toothpaste for inhibiting H. pylori according to claim 2, wherein The adjuvants comprise abrasives, thickening agents, humectants, foaming agents, cooling agents, preservatives, anticaries agents, antioxidants and bacteriostatic agents.
4. The toothpaste for inhibiting H. pylori according to claim 3, wherein The abrasives are silicon dioxide, the thickening agents are xanthan gum, the humectants comprise glycerol, propylene glycol and sorbitol, the foaming agents are sodium dodecyl sulfate, the cooling agents are menthol, the preservatives are sodium benzoate, the anticaries agents are sodium monofluorophosphate, the antioxidants are 2,6-di-tert-butyl-p-cresol and the bacteriostatic agents are domiphen bromide.
5. The toothpaste for inhibiting H. pylori according to claim 4, wherein The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract.
6. The toothpaste for inhibiting H. pylori according to any one of claims 3 to 5, wherein The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. The toothpaste comprises green tea extract, honeysuckle extract, dandelion extract and clove extract. 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