Ficus microcarpa growth inhibitor and use method thereof

Abstract

The invention discloses a ficus microcarpa growth inhibitor and a use method thereof. The ficus microcarpa growth inhibitor comprises the following effective components: 800-1200 mg / L of paclobutrazol, 1400 mg / L of chlormequat chloride and 4000 mg / L of daminozide. The growth inhibitor has the advantages that the growth inhibitor has a good inhibition effect on ficus microcarpa, the inhibition rate of newly grown branches reaches 41.83%, the times of highway pruning and maintenance are reduced, the maintenance cost is saved, the labor intensity of workers is relieved, plant leaf malformation can be reduced, and the ornamental effect of plants is guaranteed.

Description

Technical Field

[0001] This invention relates to the field of plant growth regulators, and more particularly to growth inhibitors. Background Technology

[0002] Highway greenery is typically planted in the median strip or along both sides of the road. Greenery not only beautifies the environment but also alleviates eye strain and reduces traffic accidents. In particular, median strip greenery can separate two-way traffic, accommodate buried communication cables, and provide glare reduction, thus ensuring traffic safety.

[0003] Golden banyan trees are commonly planted along highways. They grow rapidly during the rainy season, with branches sprouting quickly. Overly long branches can obstruct drivers' vision and affect driving safety. To maintain their aesthetic appeal while ensuring safety, they require multiple pruning, weeding, and fertilization processes annually. Each pruning operation requires a period of time to complete along the entire route, consuming significant manpower and resources, and also posing certain safety hazards to drivers.

[0004] There are various plant growth inhibitors on the market, which have strong inhibitory effects but also cause significant damage to plants. Plant growth inhibitors are generally applied during landscaping maintenance, usually in conjunction with pruning. After pruning, landscaping plants along highways typically retain very few leaves. Applying strong plant growth inhibitors will generally prevent new leaves from growing in the short term, and even if new leaves do grow, a high percentage will be deformed, negatively impacting the aesthetic appeal.

[0005] Therefore, developing a convenient, environmentally friendly, and highly effective plant growth inhibitor to suppress the excessive growth of the golden banyan tree while ensuring a low proportion of deformed leaves and maintaining the landscape effect has enormous market application potential. Summary of the Invention

[0006] The purpose of this invention is to provide a growth inhibitor for Ficus microcarpa and its application method, in order to solve the problems in the prior art where plant growth regulators cannot reduce the proportion of deformed leaves while ensuring the inhibition of Ficus microcarpa growth, thus affecting the landscape effect.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] A growth inhibitor for golden fig, the active ingredients of which include: 800~1200 mg / L paclobutrazol, 1400 mg / L chlormequat chloride, and 4000 mg / L butyrylhydrazine.

[0009] Furthermore, the ingredients also include adjuvants acceptable for pesticide preparation.

[0010] Furthermore, the additives include one or more of the following: wetting agents, dispersants, thickeners, defoamers, antifreeze agents, stabilizers, and water.

[0011] Furthermore, the growth inhibitor can be formulated into emulsions, suspensions, suspensions, wettable powders, or water-dispersible granules.

[0012] The present invention also provides an application of the aforementioned golden fig growth inhibitor as an inhibitor of golden fig growth.

[0013] The present invention also provides a method for using the growth inhibitor of the golden banyan tree: prune the plant one day before spraying the growth inhibitor, spray the entire leaf surface of the plant with the inhibitor until it is moist, and do not water or allow rain within 24 hours after application.

[0014] The advantages of this invention are: the growth inhibitor has a good inhibitory effect on the golden fig, with a new shoot inhibition rate of 41.83%, which reduces the number of pruning and maintenance on highways, saves maintenance costs, reduces the labor intensity of workers, and can also reduce plant leaf deformities and ensure the ornamental effect of the plant. Attached Figure Description

[0015] The accompanying drawings, which are included to provide a further understanding of the invention and form part of this application, do not constitute an undue limitation of the invention. In the drawings:

[0016] Figure 1 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the length of golden fig branches.

[0017] Figure 2 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the number of leaves on new shoots of Ficus macrophylla.

[0018] Figure 3 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the number of nodes in the new shoots of Ficus macrophylla.

[0019] Figure 4 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the internode length of new shoots of Ficus macrophylla.

[0020] Figure 5 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on deformed leaves of the golden fig.

[0021] Figure 6 These are photos showing the changes in leaf deformity after spraying with 1400mg / L paclobutrazol for one month and three months, and after spraying with commercially available plant growth inhibitors for one month and three months.

[0022] Figure 7 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the number of branches on new shoots of Ficus macrophylla.

[0023] Figure 8 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the SPAD value of *Ficus microcarpa* leaves;

[0024] Figure 9 This is a statistical chart showing the effects of different reagents, concentrations, and application methods on the fresh weight of golden fig branches;

[0025] Figure 10 This is a statistical chart showing the effect of different formulations on the length of golden fig branches;

[0026] Figure 11 This is a statistical chart showing the effect of different formulations on the number of nodes in the new shoots of the golden fig.

[0027] Figure 12 This is a statistical chart showing the effects of different formulations on deformed leaves of the golden fig.

[0028] Figure 13 This is a section of the road that verifies the spraying design drawing;

[0029] Figure 14 These are photos of the test results for applying formula A on the road section.

[0030] Figure 15 These are photos of the test results for the application of formula B on the road section.

[0031] Figure 16 These are photos of the results of the road section's verification test of formula B spraying and photos of the section without spraying. Detailed Implementation

[0032] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0033] 1. Effects of different reagent types, concentrations, and application methods on *Ficus microcarpa*

[0034] Select golden fig trees with a canopy width of 0.5~0.8 m, and use paclobutrazol and chlormequat chloride (C5H) as treatment. 13 Cl2N), butyrylhydrazide (C6H) 11 N2O3 was used as the test reagent, and water, commercially available plant growth inhibitor product 1, and commercially available plant growth inhibitor product 2 were used as controls. The specific concentrations are shown in Table 1.

[0035] The experiment employed either foliar spraying or root application. For foliar spraying, the dosage was sufficient to moisten the entire leaf surface, with 5 pots per treatment. Seedling water and fertilizer management followed normal production procedures. Pruning was performed one day prior to the experiment. After pruning, three branches from each plant were selected and marked for subsequent data measurement. The first application of the pesticide was given after pruning, followed by a second application 15 days later. No watering or rain was permitted within 24 hours of application. One month after stopping pesticide application…

[0036] The plant's morphological and physiological-biochemical indicators were tracked and observed for 3 months.

[0037] Table 1. Different application methods and concentration settings for plant growth inhibitors

[0038] Commercially available plant growth inhibitor product 1 is a package product that includes a wettable powder containing 5% clopidogrel, an agent containing 30% polyazole·methiocarbamate, and a water-soluble fertilizer containing high phosphorus and potassium macroelements.

[0039] Commercially available plant growth inhibitor product 2 is a suspension containing 24% chlormequat chloride and 6% paclobutrazol.

[0040] Test indicators:

[0041] (1) Effects of plant growth inhibitors on the length of golden fig branches;

[0042] (2) Effects of plant growth inhibitors on the number of leaves on new shoots of Ficus goldenii;

[0043] (3) Effects of plant growth inhibitors on the number of nodes in Ficus goldenii;

[0044] (4) Effects of plant growth inhibitors on the internode length of new shoots of Ficus goldenii;

[0045] (5) Effects of plant growth inhibitors on deformed leaves of Ficus microcarpa;

[0046] (6) Effects of plant growth inhibitors on the number of branches of new shoots of Ficus goldenii;

[0047] (7) Effects of plant growth inhibitors on the SPAD value of Ficus goldenii;

[0048] (8) Effect of plant growth inhibitors on the fresh weight of new shoots of Ficus goldenii.

[0049] Data processing:

[0050] (1) Basic data processing and correlation analysis. The measured data were entered and preliminarily processed using WPS XLSL spreadsheets, and correlation analysis was performed using IBM SPSS Statistics 21 software.

[0051] (2) Principal component analysis. Correlation and principal component analysis were performed on the measurement data three months after drug application, and a comprehensive evaluation was conducted using principal component factors based on the principal component analysis.

[0052] Results and Analysis:

[0053] (1) Effects of plant growth inhibitors on the length of golden fig branches

[0054] Depend on Figure 1 It can be seen that the length of new shoots of the golden fig tree differed significantly after three months under different treatments.

[0055] (p<0.05). The treatment with the longest branch length was the water control, with a length of 104.25 mm; the treatment with the shortest branch length was the commercially available plant growth inhibitor product 1, with a length of 7.83 mm, followed by the treatment with paclobutrazol 1000 mg / L (AX1), with a length of 9.24 mm; the branch length dwarfing rates were 92.49% and 91.14%, respectively, showing a significant dwarfing effect.

[0056] As shown in Table 2, compared with the water control, the dwarfing effects of different reagents on branch length varied significantly. Three months after application, the dwarfing rate of new shoots treated with paclobutrazol ranged from 79.43% to 91.14%; the dwarfing rate of new shoots treated with chlormequat chloride ranged from 4.26% to 59.71%, with only the application of 1400 mg / L chlormequat chloride (BX2) resulting in a dwarfing rate exceeding 50%; the dwarfing rate of new shoots treated with butyrylhydrazine ranged from 17.42% to 59.01%, with only the application of 4000 mg / L butyrylhydrazine (CX3) resulting in a dwarfing rate exceeding 50%. The experimental results indicate that paclobutrazol's dwarfing effect on branch length is far superior to that of chlormequat chloride and butyrylhydrazine. One and two months after application, the dwarfing effects of spraying and root application on branch length differed significantly, and at the same concentration, spraying was more effective than root application. Three months after application, there was no significant difference in the dwarfing effect of paclobutrazol spraying versus root application on branch length.

[0057] (2) Effects of plant growth inhibitors on the number of leaves on new shoots of Ficus goldenii

[0058] Depend on Figure 2 It was found that three months after application of the pesticide, there were significant differences in the number of leaves on the new shoots of *Ficus microcarpa* under different treatments (p<0.05). Spraying with 3000 mg / L butyrylhydrazine (CX2) and applying 900 mg / L chlormequat chloride to the roots (BY1) were also observed.

[0059] The newly sprouted branches treated with water had a higher number of leaves, at 12.53, 12.73, and 12.67 respectively; Root application of paclobutrazol...

[0060] The 600 mg / L (AY2) and commercially available plant growth inhibitor product 1 (CK2) had fewer leaves, with 2.80 and 3.27 tablets respectively.

[0061] As shown in Table 2, compared with the water treatment, the inhibitory effects of different reagents on the number of leaves on new shoots varied significantly. The inhibition rate of paclobutrazol was 54.78%~77.90%, that of chlormequat chloride was 0.47% (promoting)~50.04%, and that of butyrylhydrazine was 1.10%~48.93%. Paclobutrazol's inhibitory effect on the number of leaves on new shoots was much higher than that of chlormequat chloride and butyrylhydrazine.

[0062] (3) Effects of plant growth inhibitors on the number of nodes of Ficus goldenii

[0063] Depend on Figure 3 It was found that three months after application of the herbicide, there were significant differences in the number of nodes of new shoots in the *Ficus microcarpa* under different treatments (p<0.05). The herbicide was applied to the roots at 900 mg / L (BY1) and 1200 mg / L (BY2).

[0064] The newly sprouted branches treated with water had a higher number of nodes, specifically 12.00, 12.87, and 12.33 nodes respectively; Root application of paclobutrazol...

[0065] The 600 mg / L (AY2) concentration resulted in the fewest nodes on the new shoots, with 5.93 nodes.

[0066] As shown in Table 2, compared with the water control, the inhibitory effects of different reagents on the number of nodes of new shoots of Ficus microcarpa varied significantly. The inhibition rate of paclobutrazol was 38.36%~51.91%, that of chlormequat chloride was 2.68%~25.95%, and that of butyrylhydrazine was 8.60%~28.63%.

[0067] (4) Effects of plant growth inhibitors on the internode length of new shoots of Ficus goldenii

[0068] Depend on Figure 4 It was found that there were significant differences in the internode length of new shoots of *Ficus microcarpa* under different treatments (p<0.05). Root application of 900 mg / L chlormequat chloride (BY1) and 900 mg / L butyrylhydrazine (CY3) had poor inhibitory effects on the internode length of new shoots of *Ficus microcarpa*, with internode lengths of 7.63 mm and 7.27 mm, respectively. The treatment with commercially available plant growth inhibitor product 1 (CK2) resulted in the smallest internode length, at 1.25 mm.

[0069] During the three-month period after spraying paclobutrazol (AX1, AX2, AX3), commercially available plant growth inhibitor product 1 (CK2), and commercially available plant growth inhibitor product 2 (CK3), the internode length of Ficus microcarpa remained at a low level, and the inhibitory effect on the internode length of branches lasted for a long time. In contrast, the inhibition rates of chlormequat chloride and butyryl hydrazine on internode length in the first month of spraying were 25.66%~64.89% and 23.43%~51.35%, respectively. After three months, the internode length increased significantly, similar to that of the water control, indicating that the inhibitory effect was greatly reduced.

[0070] As shown in Table 2, compared with the water control, three months after application, paclobutrazol inhibited the internode length of new shoots of Ficus microcarpa by 59.92% to 83.97%, with the highest inhibition rate observed when 1000 mg / L paclobutrazol (AX1) was sprayed. Chlormequat chloride showed inhibition rates of 7.62% to 48.00%, and butyrylhydrazine showed inhibition rates of 12.00% to 41.06%. The inhibition rates of commercially available plant growth inhibitor product 1 and commercially available plant growth inhibitor product 2 were 84.84% and 82.92%, respectively.

[0071] (5) Effects of plant growth inhibitors on deformed leaves of Ficus microcarpa

[0072] Depend on Figure 5 It was found that there were significant differences in the deformed leaves of *Ficus microcarpa* under different treatments (p<0.05). (Application of pesticides)

[0073] Three months later, spray with chlormequat chloride (BX1, BX3), apply chlormequat chloride to the roots (BY2, BY3), and apply daminozide to the roots.

[0074] No deformed leaves were observed in any of the treatments. Both paclobutrazol and commercially available plant growth inhibitor product 1 resulted in a significant number of deformed leaves. The treatment with commercially available plant growth inhibitor product 1 produced the most deformed leaves (5.8 leaves), followed by the treatment with 1400 mg / L paclobutrazol (AX2) (5.2 leaves). Among all treatments, the highest teratogenic rate was observed with commercially available plant growth inhibitor product 1, which was 13.5 times higher than the water treatment. The teratogenic rate of paclobutrazol was also higher than that of chlormequat chloride and butyrylhydrazine, ranging from 6.5 to 12 times that of the water control. Furthermore, with prolonged application of chlormequat chloride and butyrylhydrazine, some deformed leaves gradually returned to normal, while the number of deformed leaves caused by paclobutrazol application actually increased over time. Figure 6 As shown, the top left image shows the state after one month of spraying with 1400 mg / L paclobutrazol (AX2), the top right image shows the state after three months of spraying with 1400 mg / L paclobutrazol (AX2), the bottom left image shows the state after one month of spraying with commercially available plant growth inhibitor product 1, and the bottom right image shows the state after three months of spraying with commercially available plant growth inhibitor product 1.

[0075] (6) Effects of plant growth inhibitors on the number of branches of new shoots of Ficus goldenii

[0076] Depend on Figure 7 It can be seen that there are significant differences in the number of new branches of Ficus goldeneri under different treatments (p<0.05).

[0077] Spray with 1400 or 1800 mg / L paclobutrazol (AX2, AX3) or 4000 mg / L butyrylhydrazine (CX3).

[0078] The most effective treatments for inhibiting branching were root application of 400 or 600 mg / L paclobutrazol (AY1, AY2), and spraying with commercially available plant growth inhibitor product 1 and product 2.

[0079] The average number of new shoots was 1, with the highest average number of branches (2.2) observed when 900 mg / L chlormequat chloride (BY1) was applied to the roots.

[0080] The second best result was achieved by spraying with 3000 mg / L butyrylhydrazine (CX2), which resulted in an average of 2.07 branches.

[0081] (7) Effects of plant growth inhibitors on the SPAD value of Ficus goldenii

[0082] Depend on Figure 8 It can be seen that the SPAD values ​​of the leaves of new shoots of Ficus microcarpa under different treatments did not differ significantly.

[0083] (p<0.05)).

[0084] (8) Effects of plant growth inhibitors on the fresh weight of new shoots of Ficus goldenii

[0085] Depend on Figure 9 It was found that there were significant differences in the fresh weight of new shoots of *Ficus microcarpa* under different treatments (p<0.05). As shown in Table 2, compared with the water treatment, the dwarfing rate of shoots treated with paclobutrazol was 84.84%–88.04%, that with chlormequat chloride was 6.36%–64.47%, and that with butyrylhydrazine was 13.04%–61.29%. The dwarfing rates of shoots treated with commercially available plant growth inhibitor product 1 and commercially available plant growth inhibitor product 2 were 92.67% and 88.32%, respectively. The effects of paclobutrazol and other existing products on the market were far superior to those of chlormequat chloride and butyrylhydrazine.

[0086] Table 2. Inhibition rates of different treatments on various indicators of Ficus microcarpa compared to the water control. Branch length Number of leaves Number of sections Intersegment length Number of branches SPAD value Fresh weight of branches Number of abnormal leaves AX1 -91.14% -61.09% -43.80% -83.97% -10.70% -13.97% -85.88% 900.00% AX2 -89.36% -54.78% -38.36% -82.09% -46.52% -2.63% -85.21% 1200.00% AX3 -90.44% -60.54% -41.61% -82.91% -46.52% -5.02% -87.38% 850.00% BX1 -43.86% -43.73% -22.71% -30.97% -25.13% -8.87% -51.81% -100.00% BX2 -59.71% -50.04% -25.95% -48.00% -39.57% -9.25% -64.47% 150.00% BX3 -30.04% -26.84% -12.98% -19.79% -25.13% -20.56% -46.54% -100.00% CX1 -32.93% -38.99% -8.60% -28.56% -42.78% -6.02% -56.21% 500.00% CX2 -25.49% -1.10% -18.90% -16.51% 10.70% -13.55% -13.04% 0.00% CX3 -59.01% -48.93% -28.63% -41.06% -46.52% -10.98% -61.29% 300.00% AY1 -81.52% -63.14% -45.42% -63.94% -46.52% -15.64% -84.84% 650.00% AY2 -87.22% -77.90% -51.91% -71.37% -46.52% -9.60% -88.04% 650.00% AY3 -79.43% -59.98% -45.90% -59.92% -42.78% -8.70% -87.07% 950.00% BY1 -4.26% 0.47% -2.68% -7.62% 17.65% -4.94% -6.36% 550.00% BY2 -13.29% -35.83% 4.38% -21.14% -32.09% -6.66% -38.93% -100.00% BY3 -19.06% -33.15% -12.98% -12.20% -21.39% -7.27% -40.84% -100.00% CY1 -23.12% -39.46% -10.79% -19.38% -28.88% -12.86% -53.88% -100.00% CY2 -23.93% -32.12% -9.73% -19.80% -35.83% -1.28% -44.32% -100.00% CY3 -17.42% -24.78% -9.73% -12.00% -18.18% -16.28% -29.10% -100.00% CK2 -92.49% -74.19% -48.66% -84.84% -46.52% -17.29% -92.67% 1350.00% CK3 -90.65% -64.72% -42.17% -82.92% -46.52% -10.87% -88.32% 500.00%

[0087] Correlation analysis:

[0088] Table 3 shows that three months after application, there were no significant relationships between SPAD values ​​and other indicators, or between the number of deformed leaves and the number of branches. However, highly significant relationships existed among the remaining indicators (P<0.01). Except for SPAD values, the other indicators played different roles in evaluating the effects of different treatments on the growth inhibition of *Ficus microcarpa*, therefore further analysis combining all indicators is necessary.

[0089] Table 3. Correlation coefficients of various indicators of Ficus microcarpa 'Golden Ficus' 3 months after application of the herbicide. index Branch length Number of leaves Number of sections Intersegment length Number of branches SPAD Fresh weight of branches Number of abnormal leaves Branch length 1 Number of leaves .893** 1 Number of sections .965** .845** 1 Intersegment length .983** .877** .919** 1 Number of branches .656** .836** .560** .627** 1 SPAD 0.222 0.234 0.29 0.162 0.055 1 Fresh weight of branches .940** .972** .879** .925** .813** 0.238 1 Number of abnormal leaves -.796** -.626** -.763** -.829** -0.387 -0.016 -.705** 1

[0090] Note: * indicates a significance level of P < 0.05, and ** indicates a significance level of P < 0.01. The same applies to the following table.

[0091] Comprehensive evaluation of the efficacy of *Ficus lyrata* based on principal component analysis:

[0092] As shown in Table 4, the values ​​measured three months after the application of the drug were processed through data standardization. Principal component analysis was performed on the various indicators under different treatments, and two principal components were extracted, with a contribution rate of 86.337%.

[0093] Table 4. Principal component analysis of various indicators of Ficus microcarpa 'Golden Ficus' 3 months after application of the herbicide.

[0094] Based on the standard values ​​of the seven indicators and the eigenvectors of the factor score coefficients of the component matrix, the regression equation for the first principal component can be derived as follows:

[0095] C1 = 0.982*X1 + 0.950*X2 + 0.939*X3 + 0.968*X4 + 0.753*X5 +0.236*X6 +

[0096] 0.979*X7 - 0.793*X8,

[0097] C1 = -0.003*X1 + 0.024*X2 + 0.087*X3 - 0.069*X4 - 0.126*X5 +0.965*X6+

[0098] 0.012*X7 - 0.227*X8

[0099] C = (0.73692*C1 + 0.12645*C2) / 0.86337

[0100] The overall score and ranking of each plant are obtained through calculation. The order of plant growth inhibition efficacy of different reagents, concentrations, and application methods is as follows: Commercially available plant growth inhibitor product 1 (spraying) > 1000 mg / L; Paclobutrazol (spraying) > 1800 mg / L; Paclobutrazol (spraying) > Commercially available plant growth inhibitor product 2 (spraying) > 1400 mg / L; Paclobutrazol (spraying) > 600 mg / L; Paclobutrazol (root application) > 400 mg / L; Paclobutrazol (root application) > 800 mg / L; Paclobutrazol (root application) > 1400 mg / L; Chlormequat chloride (spraying) > 4000 mg / L; Butyrylhydrazine (spraying) > 1000 mg / L; Chlormequat chloride (spraying) > 2000 mg / L; Butyrylhydrazine (spraying) > 1800 mg / L; Chlormequat chloride (spraying) > 500 mg / L; Butyrylhydrazine (root application) > 700 mg / L; Butyrylhydrazine (root application) > 3000 mg / L; Butyrylhydrazine (spraying) > 1500 mg / L Root application of chlormequat chloride >900mg / L; Root application of butyryl hydrazine >1200mg / L; Root application of chlormequat chloride >900mg / L; Root application of chlormequat chloride > Spray with water.

[0101] Table 5. Overall evaluation of the effects of different treatments on inhibiting the growth of Ficus microcarpa. Processing Number deal with Score (C) Ranking AX1 1000 mg / L paclobutrazol spray 20.16 2 AX2 1400 mg / L paclobutrazol spray 22.07 5 AX3 1800 mg / L paclobutrazol spray 20.87 3 BX1 1000 mg / L chlormequat chloride spray 81.68 11 BX2 1400 mg / L chlormequat chloride spray 61.45 9 BX3 1800 mg / L chlormequat chloride spray 100.09 13 CX1 2000 mg / L butyrylhydrazine spray 92.90 12 CX2 3000 mg / L butyrylhydrazine spray 109.00 16 CX3 4000 mg / L butyrylhydrazine spray 62.10 10 AY1 400 mg / L paclobutrazol root application 31.50 7 AY2 600 mg / L paclobutrazol root application 22.42 6 AY3 800 mg / L paclobutrazol root application 33.29 8 BY1 900mg / L chlormequat chloride root application 132.10 20 BY2 1200 mg / L chlormequat chloride root application 118.25 19 BY3 1500 mg / L chlormequat chloride applied to roots 111.45 17 CY1 500 mg / L butyrylhydrazine root application 105.57 14 CY2 700 mg / L butyrylhydrazine root application 106.02 15 CY3 900 mg / L butyrylhydrazine root application 115.06 18 CK1 Spray with clean water 139.09 21 CK2 1. Spray commercially available plant growth inhibitor products 14.34 1 CK3 Two commercially available plant growth inhibitor products were sprayed. 21.16 4

[0102] Summary and Discussion:

[0103] The experimental results showed that among the three reagents, paclobutrazol, chlormequat chloride, and butyrylhydrazine, paclobutrazol had the best inhibitory effect on the growth of golden fig. However, the application method and optimal concentration of paclobutrazol varied in their inhibitory effects on each indicator. Principal component analysis was used to clarify the weight of each indicator and to comprehensively evaluate the overall effect. The results showed that spraying 1000 mg / L paclobutrazol (AX1) had the best inhibitory effect.

[0104] Compared with similar products, AX1 scored higher than commercially available plant growth inhibitor product 1 (CK2) and lower than commercially available plant growth inhibitor product 2 (CK3), but the overall difference in inhibitory effect was not significant, and both had a very good inhibitory effect on golden fig.

[0105] Table 6 Comparison of the effects of different treatments on the growth inhibition of Ficus microcarpa. Comparison among the three reagents Comparison of application methods for reagents with strong inhibitory effects The strongest inhibitory concentration Compared with similar products CK2 and CK3 Branch length Paclobutrazol has the strongest inhibitory effect. Spraying is slightly more effective than root application. Spray with 1000 mg / L paclobutrazol (AX1) The difference was not significant; branch length: CK2 <AX1<CK3 Number of leaves Paclobutrazol has the strongest inhibitory effect. There was no significant difference between root application and spraying. Root application of 600 mg / L paclobutrazol (AY2) The difference was not significant; leaf number: AY2 <CK2<CK3 Number of sections Paclobutrazol has the strongest inhibitory effect. Root application is slightly more effective than spraying. Root application of 600 mg / L paclobutrazol (AY2) The difference was not significant, number of sessions: AY2 <CK2<CK3 Intersegment length Paclobutrazol has the strongest inhibitory effect. Spraying is more effective than root application Spray with 1000 mg / L paclobutrazol (AX1) The difference was not significant; intersegmental length: CK2 <AX1<CK3 Number of abnormal leaves The most malformed leaves were treated with paclobutrazol. Spraying is more common than root application. Spray with 1400 mg / L paclobutrazol (AX2) Significant differences were observed in the number of deformed leaves: CK2 > AX2 > CK3 Number of branches Paclobutrazol has the strongest inhibitory effect. Root application is slightly more effective than spraying. Spray with 1400 or 1800 mg / L paclobutrazol (AX2, AX1), spray with 4000 mg / L butyrylhydrazine (CX3), or apply 400 or 600 mg / L paclobutrazol to the roots. No difference; none of the new shoots showed any new branching. Leaf SPAD value The difference was not significant. The difference was not significant. Spray with 1800 mg / L chlormequat chloride (BX3) The difference was not significant; SPAD value: BX3 <CK2<CK3 Fresh weight of branches Paclobutrazol has the strongest inhibitory effect. The difference was not significant. Root application of 600 mg / L paclobutrazol (AY2) The differences were not significant; fresh weight of branches: CK2 > CK3 > AY2

[0106] Although the overall score of the inhibitory effect of spraying 1000 mg / L paclobutrazol (AX1) on Ficus microcarpa ranked second, lower than commercial plant growth inhibitor product 1, the dwarfing rate of new shoots after AX1 treatment was as high as 91.14%. Secondly, the number of branches and leaves of Ficus microcarpa treated with AX1 was higher than that of commercial plant growth inhibitor product 1. From the perspective of plant landscape effect, the more branches and leaves a plant has, the more compact and full the plant shape of Ficus microcarpa will be, and the better the landscape effect will be. Thirdly, the number of deformed leaves of Ficus microcarpa treated with AX1 was less than that of commercial plant growth inhibitor product 1. In subsequent compound agent tests, it is advisable to consider adding a reagent that can reduce leaf deformity.

[0107] 2. Effects of different compound growth inhibitors on *Ficus microcarpa*

[0108] Golden fig trees with a crown width of 0.5–0.8 m were selected as the experimental subjects. Seven plant growth inhibitors from four categories—carbon-containing heterocyclic compounds, ononium compounds, acylcyclohexane derivatives, and growth inhibitors—were selected as experimental reagents. Water was used as a control. The concentrations are shown in Table 7.

[0109] The experiment adopted a spraying method, with the amount sprayed enough to wet all leaf surfaces, and 5 pots per treatment. Water and fertilizer management of the seedlings was carried out according to normal production procedures. Pruning was carried out one day in advance, and after pruning, three branches from each plant were selected and marked for subsequent data measurement. The pesticide was applied once after pruning (no watering or rain within 24 hours after application). Morphological and physiological-biochemical indicators of the plants were monitored and measured every two months, with a follow-up observation period of 3 months.

[0110] Table 7. Setting of Plant Growth Inhibitors for Spraying Golden Ficus

[0111] Test indicators:

[0112] (1) Effects of plant growth inhibitors on the length of golden fig branches;

[0113] (2) Effects of plant growth inhibitors on the number of nodes and internode length of Ficus goldenii;

[0114] (3) Effects of plant growth inhibitors on deformed leaves of Ficus microcarpa;

[0115] (4) Effect of plant growth inhibitors on the number of new branches of golden fig.

[0116] Data processing:

[0117] (1) Basic data processing and correlation analysis. The measured data were entered and preliminarily processed using WPS XLSL spreadsheets, and correlation analysis was performed using IBM SPSS Statistics 21 software.

[0118] (2) Membership function analysis. All experimental data were initially processed using Excel 2003 software. Statistical analysis was performed using SAS 8.0 software. The membership function method was used to comprehensively analyze 14 leaf anatomical structure indicators; the calculation formula is: U i =(U i -U min ) / (U max -U min ), where Ui is the measured value of the index, U min U max These represent the minimum and maximum values ​​of a certain index for all experimental materials. If a certain index is negatively correlated with the heat resistance of a variety, then the inverse membership function is used for conversion, and the calculation formula is: U i =1-(U i -U min ) / (U max -U min ).

[0119] Experimental Results and Analysis:

[0120] (1) Effects of plant growth inhibitors on the length of golden fig branches

[0121] The shorter the newly sprouted branches, the stronger the inhibitory effect. Figure 10 It was found that the length of new shoots of *Ficus microcarpa* after 6 months varied significantly under different treatments (p<0.05). As shown in Table 8, the shortest shoot length was observed in treatment 7 (800 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine), with a new shoot length inhibition rate of 41.83%. Treatments 9 (800 mg / L paclobutrazol + 25 mg / L abscisic acid + 100 mg / L triiodobenzoic acid) and 3 (800 mg / L paclobutrazol + 100 mg / L uniconazole + 1400 mg / L chlormequat chloride) showed inhibition rates of 41.62% and 40.98%, respectively.

[0122] (2) Effects of plant growth inhibitors on the number of nodes and internode length of Ficus microcarpa

[0123] Depend on Figure 11Table 8 shows that the treatment with the highest average number of internodes in newly sprouted branches was treatment 14 (600 mg / L paclobutrazol + 4000 mg / L butyrylhydrazine + 25 mg / L abscisic acid), followed by treatment 8 (600 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine). The treatment with the shortest internode length was treatment 3 (800 mg / L paclobutrazol + 100 mg / L uniconazole + 1400 mg / L chlormequat chloride), with an inhibition rate of 33.31%.

[0124] (3) Effects of plant growth inhibitors on deformed leaves of Ficus microcarpa

[0125] Depend on Figure 12 It can be seen that the different treatments did not significantly affect the number of deformed leaves produced by the golden fig (p<0.05).

[0126] (4) Effects of plant growth inhibitors on the number of branches of new shoots of Ficus goldenii

[0127] The newly sprouted branches of the golden fig under different treatments did not branch.

[0128] Table 8. Inhibition rates of different treatments on various indicators of Ficus microcarpa six months after spraying inhibitors. Serial Number Average branch length (mm) Average number of internodes per branch Average internode length of branches (mm) Branch length inhibition rate (%) Intersegmental length inhibition rate (%) Branch number suppression rate (%) Inhibition rate of abnormal leaf number (%) 1 10.93 7.73 1.41 23.34% 17.86% 0 6.45% 2 12.07 8.07 1.50 15.34% 13.04% 0 2.42% 3 8.41 7.33 1.15 40.98% 33.31% 0 11.29% 4 11.32 8.60 1.32 20.60% 23.49% 0 -4.03% 5 12.18 8.47 1.44 14.51% 16.33% 0 -2.42% 6 14.90 8.60 1.73 -4.52% -0.71% 0 -4.03% 7 8.29 6.87 1.21 41.83% 29.80% 0 16.94% 8 14.36 9.47 1.52 -0.78% 11.79% 0 -14.52% 9 8.32 6.20 1.34 41.62% 21.98% 0 25.00% 10 9.76 6.47 1.51 31.52% 12.25% 0 21.77% 11 12.18 7.33 1.66 14.54% 3.44% 0 11.29% 12 10.44 7.47 1.40 26.73% 18.69% 0 9.68% 13 13.01 9.27 1.40 8.69% 18.35% 0 -12.10% 14 15.48 9.53 1.62 -8.58% 5.62% -15.32% CK 14.25 8.27 1.72

[0129] Comprehensive evaluation of the efficacy of *Ficus lyrata* based on membership function analysis:

[0130] To effectively compare the inhibitory effects of different treatments on *Ficus microcarpa*, the membership function method in fuzzy mathematics was used to comprehensively analyze various growth indicators and then make a comprehensive average. According to the ranking of the average membership values ​​of each indicator, the inhibitory effect from strongest to weakest is as follows: 7 > 3 > 1 > 9 > 4 > 5 > 12 > 2 > 13 > 14 > 10 > 8 > CK > 6 > 11. That is, the strongest comprehensive inhibitory effect is 800 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine.

[0131] Table 9. Membership function values ​​of various growth indicators of Ficus microcarpa 'Golden Bark' 6 months after spraying inhibitor. Serial Number Average branch length Average number of internodes per branch Average internode length of branches Number of branches Number of abnormal leaves Average membership value Sort 1 0.63 0.46 0.55 0.00 1.00 0.53 3 2 0.47 0.56 0.40 0.00 0.48 0.38 8 3 0.98 0.34 1.00 0.00 0.63 0.59 2 4 0.58 0.72 0.71 0.00 0.26 0.45 5 5 0.46 0.68 0.50 0.00 0.63 0.45 6 6 0.08 0.72 0.00 0.00 0.56 0.27 14 7 1.00 0.20 0.90 0.00 0.93 0.60 1 8 0.15 0.98 0.37 0.00 0.04 0.31 12 9 1.00 0.00 0.67 0.00 0.63 0.46 4 10 0.80 0.08 0.38 0.00 0.33 0.32 11 11 0.46 0.34 0.12 0.00 0.41 0.27 15 12 0.70 0.38 0.57 0.00 0.33 0.40 7 13 0.34 0.92 0.56 0.00 0.00 0.36 9 14 0.00 1.00 0.19 0.00 0.48 0.33 10 CK 0.17 0.62 0.01 0.00 0.56 0.27 13

[0132] Summary and Discussion:

[0133] The inhibitory effects of different formulations on *Ficus microcarpa* from strongest to weakest were: 7 > 3 > 1 > 9 > 4 > 5 >

[0134] 12 > 2 > 13 > 14 > 10 > 8 > CK > 6 > 11. The most effective inhibitory combination was 800 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine, achieving a 41.83% inhibition rate on newly sprouted branches. The next most effective combination was...

[0135] The combination of 800 mg / L paclobutrazol, 100 mg / L uniconazole, and 1400 mg / L chlormequat chloride resulted in a 40.98% inhibition rate on newly emerging branches.

[0136] The highest inhibition rate of the golden fig branches was 41.83%, which may be due to the following reasons:

[0137] 1) The concentration of paclobutrazol was too low. Spraying paclobutrazol at 1200 mg / L was more effective than spraying at 800 mg / L and 1000 mg / L. During the road section verification, the formula was adjusted to 1200 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine.

[0138] 2) The experimental seedlings were watered by drip irrigation. During the experiment, the drip irrigation system of the control treatment became clogged, which resulted in slower growth of the control treatment and thus affected the experimental results.

[0139] 3. Validation of composite inhibitor road sections

[0140] The test section was planted with golden fig trees, 2 meters wide, and sprayed from both directions. A 16 L sprayer was used, and one tank of pesticide could cover 100 meters in both directions. The section was pruned and sprayed as required. Before spraying, the plant height in the verification section was 85-90 cm, and the overall crown width was 170 cm.

[0141] Experimental formulation

[0142] (1) A: 800 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine, sprayed over 300 m;

[0143] (2) B: 1200 mg / L paclobutrazol + 1400 mg / L chlormequat chloride + 4000 mg / L butyrylhydrazine, sprayed over 300 m;

[0144] There was no rain within 24 hours of the application of the pesticide.

[0145] Road section verification spraying design such as Figure 13 As shown.

[0146] The fungicide was applied in October of the previous year, and the growth of the golden fig was as follows in February of the following year. Figure 14-16 As shown. Figure 14 The image shown is a photograph taken using formula A. (As shown...) Figure 15 The image shown is a photograph taken using formula B. Figure 16As shown, the area corresponding to the front of the blue car traveling in the opposite lane is the boundary between the test section and the non-test section. The area in the direction the blue car is traveling is the test section, where formula B is sprayed. The area that the blue car has already passed is the non-test section, where no pesticide is applied. It is obvious that the golden banyan trees without pesticides are growing much better than those sprayed with formula B. In addition, these figures show that formula B is more effective than formula A.

[0147] The technical solutions provided by the embodiments of the present invention have been described in detail above. Specific examples have been used to illustrate the principles and implementation methods of the embodiments of the present invention. The descriptions of the embodiments above are only for helping to understand the principles of the embodiments of the present invention. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the embodiments of the present invention. Therefore, the content of this specification should not be construed as a limitation of the present invention.

Claims

1. A growth inhibitor for the golden fig, characterized in that, Its active ingredients include: 800-1200 mg / L paclobutrazol, 1400 mg / L chlormequat chloride, and 4000 mg / L butyrylhydrazine.

2. The golden fig growth inhibitor according to claim 1, characterized in that, The ingredients also include adjuvants acceptable for pesticide preparation.

3. The golden fig growth inhibitor according to claim 2, characterized in that, Additives include one or more of the following: wetting agents, dispersants, thickeners, defoamers, antifreeze agents, stabilizers, and water.

4. The golden fig growth inhibitor according to claim 2, characterized in that, Growth inhibitors can be formulated into emulsions, suspensions, suspensions, wettable powders, or water-dispersible granules.

5. The use of the golden fig growth inhibitor as described in any one of claims 1-4 as an inhibitor of golden fig growth.

6. A method of using the golden fig growth inhibitor as described in claim 1, characterized in that: Prune the plant one day before spraying the growth inhibitor, spray the entire leaf surface of the plant with the inhibitor until it is moist, and do not water or allow rain within 24 hours after application.

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