Feed for improving color and luster of eggs and content of natural carotene as well as preparation method and application of feed

Abstract

The invention belongs to the technical field of feed. The invention provides a feed for improving the color and luster of eggs and the content of natural carotene as well as a preparation method and application of the feed. The feed comprises a basal feed and a feed additive, wherein the feed additive comprises blakeslea trispora and chili powder. The egg yolk chromaticity of eggs and the content of beta-carotene in the egg yolk can be remarkably improved. Meanwhile, the total egg protein content can be increased, the total cholesterol and moisture content can be reduced, and the technical effect of improving the egg quality is achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of feed, and particularly relates to a feed for improving egg color and natural carotene content, and a preparation method and application thereof. BACKGROUND

[0002] Currently, in the egg breeding industry, chemical synthetic pigment feed additives such as canthaxanthin and apo-8'-carotenoic acid ethyl ester are widely used to improve egg yolk color, but excessive dosage and long-term use can cause different degrees of damage to the muscle tissue, liver and kidney organs of laying hens, and can cause human retinal macular pigment deposition disease, exacerbate hyperactivity in children and induce other diseases. Natural carotenoids as animal feed additives can effectively reduce the harm to human food safety caused by the use of chemical pigments, but their improvement effect on egg yolk color is not good, and high dosage also does not have the effect of improving egg quality, so that the economy of the industrial application is poor, which is not conducive to popularization and use. SUMMARY

[0003] In view of the defects of the prior art, the purpose of the present application is to provide a feed capable of significantly improving egg color and natural carotene content, and a preparation method and application thereof.

[0004] In order to achieve the above purpose, the present application provides the following scheme: A feed for improving egg color and natural carotene content, the feed comprising a basic feed and a feed additive, the feed additive comprising Blakeslea trispora and pepper powder.

[0005] Preferably, the weight fraction of the Blakeslea trispora in the feed is 0.08-0.4%.

[0006] Preferably, the weight fraction of the pepper powder in the feed is 1-2%.

[0007] Preferably, the weight fraction of the Blakeslea trispora in the feed is 0.08%, and the weight fraction of the pepper powder in the feed is 2%.

[0008] Preferably, the weight fraction of the Blakeslea trispora in the feed is 0.24%, and the weight fraction of the pepper powder in the feed is 1.5%.

[0009] Preferably, the basic feed comprises corn, soybean meal, fermented rapeseed meal, soybean oil, stone powder, premix, corn protein powder and pine needle powder.

[0010] Preferably, the premix provides 1000 mg of NaCl, 12000 IU of VA, 4000 IU of VD3, 20 mg of VE, 2.0 mg of VK3, 1.0 mg of thiamine, 8.5 mg of riboflavin, 8.0 mg of pantothenic acid, 32.0 mg of nicotinic acid, 7.0 mg of pyridoxine, 0.8 mg of biotin, 5.0 mg of folic acid, 110 mg of choline, 0.02 mg of VB D 0.02 mg, 60.0 mg of Mn, 55 mg of Fe, 6.0 mg of Cu, 62.0 mg of Zn and 0.4 mg of Se per kg of feed. 12 0.02 mg, 60.0 mg of Mn, 55 mg of Fe, 6.0 mg of Cu, 62.0 mg of Zn and 0.4 mg of Se per kg of feed.

[0011] Preferably, the feed comprises 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn gluten meal, 2% pine needle powder, and the rest is corn and feed additives.

[0012] A method for preparing a feed for improving the color and natural carotene content of eggs, the method comprising mixing the base feed and the feed additives in the aforementioned feed.

[0013] The aforementioned feed for improving the color and natural carotene content of eggs.

[0014] Advantages of the present application: The present application can significantly improve the yolk color and the content of β-carotene in the yolk of eggs. At the same time, it can also improve the total protein content of eggs, reduce the total cholesterol and water content, and has the technical effect of improving the quality of eggs. DETAILED DESCRIPTION

[0015] The present application will be described in detail below through examples. It is necessary to point out here that the following examples are only used to further illustrate the present application and cannot be understood as limiting the protection scope of the present application. Some non-essential improvements and adjustments made by skilled persons in the art based on the above description of the present application still belong to the protection scope of the present application.

[0016] Example 1 The raw materials used in this example are as follows: Blakeslea trispora dry bacterial bodies (β-carotene content: 2%, number of bacterial bodies: 6.4 x 10 10 CFU / g), provided by Ji Biyou Biotechnology (Wuhan) Co., Ltd., and chili powder purchased from a local farmers' market.

[0017] The feed was prepared according to the following weight fractions: 57.42% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn protein powder, 2% pine needle powder, 0.08% Trichoderma harzianum, 2% paprika powder.

[0018] Example 2 The raw materials used in this example are as follows: Trichoderma harzianum dry spores (β-carotene content: 2%, spore quantity: 6.4 x 10 10 CFU / g), provided by Ji Biyou Biotechnology (Wuhan) Co., Ltd.; paprika powder was purchased from the local farmers' market.

[0019] The feed was prepared according to the following weight fractions: 57.76% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn protein powder, 2% pine needle powder, 0.24% Trichoderma harzianum, 1.5% paprika powder.

[0020] Example 3 The raw materials used in this example are as follows: Trichoderma harzianum dry spores (β-carotene content: 2%, spore quantity: 6.4 x 10 10 CFU / g), provided by Ji Biyou Biotechnology (Wuhan) Co., Ltd.; paprika powder was purchased from the local farmers' market.

[0021] The feed was prepared according to the following weight fractions: 57.26% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn protein powder, 2% pine needle powder, 0.24% Trichoderma harzianum, 2% paprika powder.

[0022] Example 4 The raw materials used in this example are as follows: Trichoderma harzianum dry spores (β-carotene content: 2%, spore quantity: 6.4 x 10 10 CFU / g), provided by Ji Biyou Biotechnology (Wuhan) Co., Ltd.; paprika powder was purchased from the local farmers' market.

[0023] The feed was prepared according to the following weight fractions: 57.6% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn protein powder, 2% pine needle powder, 0.4% Trichoderma harzianum, 1.5% paprika powder.

[0024] Example 5 The raw materials used in this example are as follows: Trichoderma reesei dry cell mass (β-carotene content: 2%, cell mass amount: 6.4 x 10 10 CFU / g), provided by Ji Biyou Biotechnology (Wuhan) Co., Ltd.; paprika was purchased from a local farmers' market.

[0025] The feed was prepared according to the following weight fractions: 57.1% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn gluten meal, 2% pine needle powder, 0.4% Trichoderma reesei, 2% paprika.

[0026] Comparative Example 1 The feed was prepared according to the following weight fractions: 56.7% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn gluten meal, 2% pine needle powder, 1.3% calcium bicarbonate, 1.5% paprika.

[0027] Comparative Example 2 The basal feed was prepared according to the following weight fractions: 57.96% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn gluten meal, 2% pine needle powder, 1.3% calcium bicarbonate, 0.24% Trichoderma reesei.

[0028] Comparative Example 3 The feed was prepared according to the following weight fractions: 58.2% corn, 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% stone powder, 3% premix, 3% corn gluten meal, 2% pine needle powder, 1.3% calcium bicarbonate.

[0029] Experimental Example 1 Healthy, 42-week-old Hy-Line gray laying hens weighing similarly were selected for the test, with 800 hens in total, which were randomly divided into 8 groups, with 5 replicates in each group and 20 hens in each replicate. The test design was divided into feeding of Examples 1-5 and Comparative Examples 1-3, a total of 8 groups, and the test date was from April 16, 2025 to May 20, 2025, for a total of 35 days, with a pre-feeding period of 5 days and an official test period of 30 days.

[0030] At the end of the test, 20 eggs were randomly collected from each group, and the content of β-carotene in the yolk of each sample was detected according to the “National Food Safety Standard Determination of Carotene in Food” (GB5009.83-2016), and the yolk color was determined by using a Roche colorimetric fan.

[0031] The experimental results are shown in Table 1.

[0032] Table 1

[0033] Experimental Example 2 The nutritional indexes of the group fed with the feed of Example 2 in Experimental Example 1 were detected, taking Comparative Example 1 as Control 1 and Comparative Example 3 as Control 2. The protein, fat, cholesterol and moisture contents in the eggs were detected according to the National Food Safety Standard for Determination of Protein in Foods (GB5009.5-2016), the National Food Safety Standard for Determination of Fat in Foods (GB5009.5-2016), the National Food Safety Standard for Determination of Cholesterol in Foods (GB5009.128-2016) and the National Food Safety Standard for Determination of Moisture in Foods (GB5009.3-2016).

[0034] The results are shown in Table 2. Compared with Control 2, the total protein content of the group of Example 2 increased by 9.08% (P<0.05), the total cholesterol and moisture contents decreased by 28.00% (P<0.05) and 2.82% (P<0.01), respectively, and the total fat content had no significant change; the same effect was also observed compared with Control 1. The test results show that the present application can improve the nutritional quality of eggs.

[0035] Table 2

Claims

1. A feed for improving egg color and natural carotene content, characterized in that, The feed includes a base feed and feed additives, the feed additives including Bacillus trispora and chili powder.

2. The feed for improving egg color and natural carotene content according to claim 1, characterized in that, The weight fraction of *Blancotomyces trispora* in the feed is 0.08-0.4%.

3. A feed for improving egg color and natural carotene content according to claim 1 or 2, characterized in that, The chili powder is present in the feed at a weight fraction of 1-2%.

4. The feed for improving egg color and natural carotene content according to claim 3, characterized in that, The *Blancium trispora* was present in the feed at a weight fraction of 0.08%, and the chili powder was present in the feed at a weight fraction of 2%.

5. The feed for improving egg color and natural carotene content according to claim 3, characterized in that, The *Blancium trispora* was present in the feed at a weight fraction of 0.24%, and the chili powder was present in the feed at a weight fraction of 1.5%.

6. The feed for improving egg color and natural carotene content according to claim 1, characterized in that, The basic feed includes corn, soybean meal, fermented rapeseed meal, soybean oil, limestone powder, premix, corn gluten meal, and pine needle powder.

7. The feed for improving egg color and natural carotene content according to claim 6, characterized in that, The premix provides 1000 mg NaCl, 12000 IU VA, 4000 IU VD3, 20 mg VE, 2.0 mg VK3, 1.0 mg thiamine, 8.5 mg riboflavin, 8.0 mg pantothenic acid, 32.0 mg niacin, and 7.0 mg pyridoxine per kg of feed. D - Biotin 0.8 mg, folic acid 5.0 mg, choline 110 mg, vitamin B12 0.02 mg, Mn 60.0 mg, Fe 55 mg, Cu 6.0 mg, Zn 62.0 mg and Se 0.4 mg.

8. A feed for improving egg color and natural carotene content according to claim 6 or 7, characterized in that, The feed, by weight, includes 20% soybean meal, 3% fermented rapeseed meal, 1% soybean oil, 8.5% limestone powder, 3% premix, 3% corn gluten meal, 2% pine needle powder, and the remainder is corn and feed additives.

9. A method for preparing feed that improves egg color and natural carotene content, characterized in that, The preparation method involves mixing the basic feed and feed additives in the feed according to any one of claims 1 to 8.

10. The use of the feed as described in any one of claims 1 to 8 in improving the color and natural carotene content of eggs.

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