Double-layer essence with anti-aging effect and preparation method thereof

Abstract

The invention relates to the technical field of skin care essence, in particular to double-layer essence with an anti-aging effect and a preparation method thereof.The double-layer essence with the anti-aging effect is composed of a water phase layer and an oil phase layer; the water-phase layer is prepared from the following raw materials in percentage by mass: 3-8% of hydroxypropyl tetrahydropyrantriol, 0.1-0.5% of tetrahydromethylpyrimidine carboxylic acid, 0.01-0.1% of a pepper kava extract, 1-5% of lactobacillus / soybean milk fermentation product filtrate, 1-5% of vitamin C ethyl ether, 1-5% of acetylchitosamine, 3-8% of 1, 3-butanediol and the balance of water. The invention relates to an anti-aging mask which comprises the following components in percentage by weight: 1-10% of 1, 3-butanediol, 0.01-0.1% of sodium hyaluronate, 0.1-0.5% of a buffer system, 5-10% of glycerol and the balance of deionized The oil phase layer is prepared from the following raw materials: 0.5-2% of ascorbyl tetraisopalmitate, 11-44% of compound plant-derived grease and 0.05-0.3% of tocopheryl acetate, and the problems that existing essence oil is lack of a multi-dimensional synergistic anti-aging effect, and the stability and transdermal absorption efficiency of water-soluble active ingredients and oil-soluble ingredients are difficult to consider are solved.

Description

Technical Field

[0001] This invention relates to the field of skincare essence technology, and in particular to a double-layer essence with anti-aging effects and its preparation method. Background Technology

[0002] As we age, our bodies begin to age, primarily manifesting as skin aging. For example, the supporting structure of the skin gradually weakens, causing it to become loose and lose its firmness; the ability of muscles to contract weakens, causing wrinkles to gradually deepen and eventually form static wrinkles; and the skin's natural moisturizing factors decrease, leading to a decline in the skin's ability to retain water and making it dry. The main signs of skin aging are reduced elasticity, wrinkles, sagging, and drooping. Therefore, anti-aging serums have a promising market prospect.

[0003] However, most facial oil products on the market are pure oil phase or water-oil emulsion systems, which make it difficult to balance the stability and transdermal absorption efficiency of water-soluble active ingredients and oil-soluble ingredients. Moreover, active ingredients are easily degraded by external factors, resulting in weakened product efficacy. Most importantly, existing anti-aging products on the market focus on single anti-wrinkle or moisturizing effects, lacking multi-dimensional synergistic anti-aging solutions. Summary of the Invention

[0004] To address the aforementioned shortcomings, the present invention aims to propose a dual-layer essence with anti-aging effects and its preparation method, thereby solving the problems of existing essence oils lacking multi-dimensional synergistic anti-aging effects and difficulty in balancing the stability and transdermal absorption efficiency of water-soluble active ingredients and oil-soluble ingredients.

[0005] To achieve this objective, the present invention adopts the following technical solution: A dual-layer essence with anti-aging effects, the dual-layer essence being composed of an aqueous phase layer and an oil phase layer; The raw materials for preparing the aqueous phase layer, calculated by mass percentage, include 3-8% hydroxypropyl tetrahydropyranotriol, 0.1-0.5% tetrahydromethylpyrimidine carboxylic acid, 0.01-0.1% kava pepper extract, 1-5% lactobacillus / soybean fermentation product filtrate, 1-5% vitamin C ethyl ether, 1-5% acetylglucosamine, 3-8% 1,3-butanediol, 0.01-0.1% sodium hyaluronate, 0.1-0.5% buffer system, 5-10% glycerol, and the balance deionized water. The raw materials for preparing the oil phase include 0.5-2% ascorbic acid tetraisopalmitate, 11-44% compounded plant-derived oils, and 0.05-0.3% tocopheryl acetate.

[0006] Preferably, the compounded plant-derived oils, calculated as a percentage of the total raw materials by mass, include 5-15% plant squalane, 3-10% jojoba seed oil, 2-8% camellia seed oil, 1-5% sunflower seed oil, 0.5-3% wild soybean oil, 0.1-1% meadowfoam seed oil, 0.1-1% milk thistle seed oil, and 0.1-1% macadamia seed oil.

[0007] Furthermore, the buffer system is a citric acid / sodium citrate buffer system, with a molar ratio of citric acid to sodium citrate of 1:(1.5-2.5).

[0008] Furthermore, the mass ratio of the aqueous phase layer to the oil phase layer is (2-4):1.

[0009] Preferably, the sodium hyaluronate comprises large-molecule sodium hyaluronate and small-molecule sodium hyaluronate, and the mass ratio is (1-1.5):1.

[0010] A method for preparing a double-layer essence with anti-aging effects according to any one of the above methods includes the following steps: S1. Aqueous phase preparation: At 80-85℃, 1,3-butanediol and sodium hyaluronate are added to an aqueous phase reactor containing water and stirred until dissolved. Then, glycerol and a buffer system are added, and stirring is continued until dissolved. The temperature is lowered to 40-45℃, and vitamin C ethyl ether, acetyl glucosamine, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, lactobacillus / soy milk fermentation product filtrate, and kava pepper extract are added to the aqueous phase reactor and stirred until dissolved. The temperature is lowered to 35℃ and filtered to obtain an aqueous phase mixture. S2. Oil phase preparation: At room temperature, plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil are added into a dry oil phase reaction vessel, stirred and dissolved evenly, and filtered to obtain an oil phase mixture. S3. Mixing and filling: Slowly add the oil phase mixture to the aqueous phase mixture, stir evenly, and then fill to obtain a double-layer essence.

[0011] Preferably, in step S1, sodium hyaluronate and 1,3-butanediol need to be pre-mixed and dispersed before being added to the aqueous reaction vessel; Vitamin C ethyl ether, acetylglucosamine, hydroxypropyl tetrahydropyranotriol and tetrahydromethylpyrimidine carboxylic acid need to be pre-mixed, dispersed and dissolved in water before being added to the aqueous reaction vessel.

[0012] Preferably, in step S1, the homogenization rate of 1,3-butanediol and sodium hyaluronate, which are pre-mixed and dispersed at 80-85°C, is 2500-3000 r / min, and the homogenization time is 1-3 min.

[0013] The technical solution provided by this invention may include the following beneficial effects: Hydroxypropyl tetrahydropyranotriol promotes collagen production, ascorbate tetraisopalmitate inhibits collagen degradation and has antioxidant properties, tetrahydromethylpyrimidine carboxylic acid repairs the skin barrier, Lactobacillus / soy milk fermentation product filtrate regulates the microecology, and kava pepper extract provides soothing effects. Together, they cover the three major anti-aging pathways of anti-wrinkle, anti-oxidation, and repair, achieving multi-dimensional synergistic anti-aging. In addition, vitamin C ethyl ether, acetylglucosamine, 1,3-butanediol, and sodium hyaluronate are included. Through the synergistic effect of interfacial activity, stable dispersion can be achieved after mixing the oil phase and the water phase, ensuring that the active ingredients can penetrate into the skin efficiently and improve transdermal absorption efficiency. At the same time, the entire system forms a double layer after standing, protecting both water-soluble and oil-soluble active ingredients. Detailed Implementation

[0014] The technical solution of the present invention will be further illustrated below through specific embodiments.

[0015] To facilitate understanding of the present invention, a more complete description is provided below. The present invention can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to provide a thorough and complete understanding of the disclosure of the present invention.

[0016] Where specific techniques or conditions are not specified in the examples, they shall be performed in accordance with the techniques or conditions described in the literature in this field or in accordance with the product instructions. Reagents or instruments whose manufacturers are not specified are all commercially available conventional products.

[0017] A dual-layer essence with anti-aging effects, the dual-layer essence being composed of an aqueous phase layer and an oil phase layer; The raw materials for preparing the aqueous phase layer, calculated by mass percentage, include 3-8% hydroxypropyl tetrahydropyranotriol, 0.1-0.5% tetrahydromethylpyrimidine carboxylic acid, 0.01-0.1% kava pepper extract, 1-5% lactobacillus / soybean fermentation product filtrate, 1-5% vitamin C ethyl ether, 1-5% acetylglucosamine, 3-8% 1,3-butanediol, 0.01-0.1% sodium hyaluronate, 0.1-0.5% buffer system, 5-10% glycerol, and the balance deionized water. The raw materials for preparing the oil phase include 0.5-2% ascorbic acid tetraisopalmitate, 11-44% compounded plant-derived oils, and 0.05-0.3% tocopheryl acetate.

[0018] Skin aging manifests as decreased elasticity, increased wrinkles, sagging, and dryness. This invention proposes a dual-layer essence with anti-aging effects. Compared to products on the market that focus on single anti-wrinkle or moisturizing effects, this invention achieves anti-aging through multi-dimensional synergy. The functional ingredients included in this dual-layer essence are hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, kava pepper extract, lactobacillus / soy milk fermentation product filtrate, and ascorbate tetraisopalmitate. For skin whose supporting structure is weakened, leading to loss of firmness, the hydroxypropyltetrahydropyranotriol in this invention can activate the synthesis of glycosaminoglycans (GAGs) in the epidermal-dermal junction (DEJ). Glycosaminoglycans can stimulate collagen production, promote the orderly arrangement of collagen fibers, further enhance the skin's supporting structure, and improve skin firmness. Simultaneously, it can promote the expression of type IV and type VII collagen, effectively improving skin elasticity. Meanwhile, ascorbate tetraisopalmitate can neutralize free radicals and inhibit collagen degradation. The synergistic effect of ascorbate tetraisopalmitate and hydroxypropyltetrahydropyranotriol can stimulate collagen production and inhibit collagen degradation, making the skin firmer and achieving anti-wrinkle effects. Furthermore, free radical damage caused by aging leads to skin oxidation, increased melanin production, and consequently, dull skin tone and age spots. As a fat-soluble derivative of vitamin C, ascorbate tetraisopalmitate can penetrate the stratum corneum and be converted into active vitamin C within the skin, inhibiting tyrosinase activity, reducing melanin production, effectively brightening skin tone, and correspondingly reducing age spots. Meanwhile, tetrahydromethylpyrimidine carboxylic acid can stabilize protein structures and cell membranes, resist environmental stresses such as ultraviolet rays and pollution, repair the skin barrier, and reduce skin moisture loss. Lactobacillus / soy ferment filtrate, rich in small molecule peptides and organic acids, can regulate the skin's microbiome balance, enhance stratum corneum cohesion, and improve rough skin texture. Tetrahydromethylpyrimidine carboxylic acid and Lactobacillus / soy ferment filtrate synergistically regulate the skin's microecology, alleviate redness and dryness, effectively compensate for skin dryness caused by a reduction in the skin's natural moisturizing factors, achieve moisturizing effects, repair the skin barrier, and brighten the complexion while minimizing product irritation. Additionally, it includes kava pepper extract, which is rich in kava lactone. This product can inhibit the expression of COX-2 inflammatory factors, reduce skin redness and irritation, and promote the release of β-endorphin to soothe nerve sensitivity. It significantly reduces the burning sensation after product use, further reducing the irritation of the product and meeting user needs. This invention promotes collagen production through hydroxypropyl tetrahydropyranotriol, inhibits collagen degradation and has antioxidant properties through ascorbic acid tetraisopalmitate, repairs the skin barrier through tetrahydromethylpyrimidine carboxylic acid, regulates the microecology through lactobacillus / soy milk fermentation product filtrate, and achieves soothing effects through kava pepper extract. The above components cover three major anti-aging pathways: anti-wrinkle, antioxidant, and repair, achieving multi-dimensional synergistic anti-aging and solving the problem that existing essential oils lack multi-dimensional synergistic anti-aging effects.

[0019] It also includes vitamin C ethyl ether, acetyl glucosamine, 1,3-butanediol, and sodium hyaluronate. When using this dual-layer essence, it needs to be shaken to mix the oil phase and the water phase. These four ingredients work synergistically through interfacial activity to achieve stable dispersion after the oil and water phases are mixed. Among them, 1,3-butanediol is a small molecule polyol with both water solubility and weak lipophilicity, which promotes the dispersion of oil droplets in the oil phase. Sodium hyaluronate is a water-soluble high molecular weight polysaccharide with an extended long chain structure in the water phase. The shear force generated by shaking promotes the extension of the molecular chains of sodium hyaluronate to wrap around the dispersed oil droplets. Acetyl glucosamine is a weakly cationic ingredient that can enhance the adsorption of sodium hyaluronate on the surface of oil droplets and stabilize the oil droplets. Vitamin C ethyl ether forms a weak interaction with 1,3-butanediol and acetyl glucosamine, enhancing the binding force between oil droplets and the aqueous phase. These four components achieve a temporary emulsified state after shaking and are free of emulsifiers. The texture is light and thin, achieving an oil droplet size of 1-5mm, ensuring that the active ingredients can penetrate the skin efficiently and improving transdermal absorption efficiency. Furthermore, due to the lack of continuous external force and strong interaction, the emulsified system returns to a thermodynamically stable bilayer structure after shaking, ensuring the stability of both water-soluble and oil-soluble active ingredients, preventing oxidation of the aqueous active ingredients, extending the product's shelf life, and solving the problem that existing essence oils cannot simultaneously achieve the stability of both water-soluble and oil-soluble active ingredients and transdermal absorption efficiency.

[0020] Furthermore, limiting the mass percentages of vitamin C ethyl ether, acetylglucosamine, 1,3-butanediol, and sodium hyaluronate ensures the stability of the bilayer structure and guarantees the formation of a homogeneous suspension system after shaking.

[0021] Therefore, this dual-layer essence addresses the shortcomings of existing serums in achieving multi-dimensional synergistic anti-aging effects and balancing the stability and transdermal absorption efficiency of both water-soluble and oil-soluble active ingredients by incorporating appropriate amounts of ascorbic acid tetraisopalmitate, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, kava pepper extract, Lactobacillus / soybean ferment filtrate, vitamin C ethyl ether, acetylglucosamine, 1,3-butanediol, and sodium hyaluronate.

[0022] In addition, it includes a buffer system that can improve the compatibility and gentleness of the dual-layer essence, maintain overall stability, and ensure that each raw material component can exist stably. Glycerin works synergistically with tetrahydromethylpyrimidine carboxylic acid to achieve the effect of moisturizing, while tocopheryl acetate has an antioxidant effect, further working together to repair the skin barrier, achieve brightening effect, and meet the purpose of anti-aging.

[0023] Preferably, the compounded plant-derived oils, calculated as a percentage of the total raw materials by mass, include 5-15% plant squalane, 3-10% jojoba seed oil, 2-8% camellia seed oil, 1-5% sunflower seed oil, 0.5-3% wild soybean oil, 0.1-1% meadowfoam seed oil, 0.1-1% milk thistle seed oil, and 0.1-1% macadamia seed oil.

[0024] Specifically, plant-based squalane can mimic the structure of the sebum membrane, penetrate quickly, and enhance barrier function, while jojoba seed oil can regulate sebum secretion, improve enlarged pores, and has both antioxidant and anti-inflammatory effects. Plant-based squalane and jojoba seed oil form a "biomimetic lipid layer" for better long-lasting hydration. Camellia seed oil has high permeability, promoting the absorption of active ingredients. Its tea polyphenols can scavenge free radicals and delay photoaging, while milk thistle seed oil also has strong antioxidant properties, inhibiting lipid peroxidation and protecting cell membrane integrity. Camellia seed oil and milk thistle seed oil respectively... Water-soluble and fat-soluble antioxidants cover the entire spectrum of free radical scavenging. Wild soybean oil has anti-inflammatory and soothing properties, inhibits melanin transfer, and evens out skin tone, while macadamia seed oil promotes wound healing and enhances skin elasticity. Both wild soybean oil and macadamia seed oil are rich in sterols, which accelerate barrier repair and reduce environmental damage. Sunflower seed oil repairs the lipid arrangement of the stratum corneum and relieves dryness, while meadowfoam seed oil has excellent stability, forming a breathable protective film to prevent moisture loss, further assisting plant squalane and jojoba seed oil in hydration. Therefore, plant squalane, jojoba seed oil, sunflower seed oil, and meadowfoam seed oil enhance moisturizing effects; camellia seed oil and milk thistle seed oil form an antioxidant matrix; and wild soybean oil and macadamia seed oil promote barrier repair. The combined use of these plant oils mimics the skin's lipid structure, enhancing moisturizing and barrier repair functions. This blend of plant-derived oils, synergistically with kava extract, further achieves the goal of barrier repair, improves skin radiance, and better achieves anti-aging effects.

[0025] Furthermore, the buffer system is a citric acid / sodium citrate buffer system, with a molar ratio of citric acid to sodium citrate of 1:(1.5-2.5).

[0026] Specifically, the buffer system is a buffer system composed of citric acid and sodium citrate, and at this molar ratio, the overall pH value is maintained at 5.0-6.0, protecting the active ingredients in the aqueous phase layer, effectively prolonging the effective time of the active ingredients, and at this pH value, it is close to the natural acid-base environment of the skin, which can reduce the risk of skin irritation caused by pH value, and is relatively mild.

[0027] At the same time, citric acid can chelate metal ions, such as copper ions and ferrous ions, block free radical chain reactions, protect ascorbic acid tetraisopalmitate and compound plant-derived oils in the oil phase, and also inhibit the activity of matrix metalloproteinases (MMPs), slow down collagen degradation, and achieve anti-aging.

[0028] Furthermore, the mass ratio of the aqueous phase layer to the oil phase layer is (2-4):1.

[0029] Specifically, at this mass ratio, it is ensured that the mixture of the aqueous and oil phases provides a refreshing and non-greasy skin feel when applied to the skin.

[0030] Preferably, the sodium hyaluronate comprises large-molecule sodium hyaluronate and small-molecule sodium hyaluronate, and the mass ratio is (1-1.5):1.

[0031] Specifically, small-molecule sodium hyaluronate can penetrate the skin efficiently to achieve the effect of moisturizing, while large-molecule sodium hyaluronate can form a film on the skin surface to effectively lock in moisture. The combined effect of large-molecule and small-molecule sodium hyaluronate takes into account both film formation and penetration functions, thereby improving the moisturizing effect.

[0032] Among them, the molecular weight of high molecular weight sodium hyaluronate is 1000-1500kDa, and the molecular weight of low molecular weight sodium hyaluronate is 5-50kDa.

[0033] Preferably, the molecular weight of the high molecular weight sodium hyaluronate is 1200 kDa, and the molecular weight of the low molecular weight sodium hyaluronate is 5 kDa.

[0034] A method for preparing a double-layer essence with anti-aging effects according to any one of the above methods includes the following steps: S1. Aqueous phase preparation: At 80-85℃, 1,3-butanediol and sodium hyaluronate are added to an aqueous phase reactor containing water and stirred until dissolved. Then, glycerol and a buffer system are added, and stirring is continued until dissolved. The temperature is lowered to 40-45℃, and vitamin C ethyl ether, acetyl glucosamine, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, lactobacillus / soy milk fermentation product filtrate, and kava pepper extract are added to the aqueous phase reactor and stirred until dissolved. The temperature is lowered to 35℃ and filtered to obtain an aqueous phase mixture. S2. Oil phase preparation: At room temperature, plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil are added into a dry oil phase reaction vessel, stirred and dissolved evenly, and filtered to obtain an oil phase mixture. S3. Mixing and filling: Slowly add the oil phase mixture to the aqueous phase mixture, stir evenly, and then fill to obtain a double-layer essence.

[0035] Specifically, according to the above steps, ensure that all ingredients are mixed evenly, ensure that the activity of each raw material component is not destroyed, ensure that the double-layer essence can achieve the corresponding anti-aging effect, and also ensure that the double-layer visual effect is achieved.

[0036] In this process, steps S1 and S2 are not sequential. In step S3, the oil phase mixture is added to the aqueous phase mixture to facilitate the formation of oil droplets that are dispersed in the aqueous phase. If the aqueous phase mixture is added to the oil phase mixture, due to the high viscosity of the oil phase mixture, larger water droplets will be formed, resulting in uneven dispersion and affecting the subsequent formation of the bilayer structure.

[0037] Preferably, in step S2, each component of the oil phase is added sequentially to the dry oil phase reactor, and the mixture is stirred until homogeneous after each addition. This sequential addition further ensures that each component is uniformly dispersed throughout the oil phase.

[0038] Preferably, in step S1, sodium hyaluronate and 1,3-butanediol need to be pre-mixed, dispersed, and dissolved before being added to the aqueous reaction vessel; Vitamin C ethyl ether, acetylglucosamine, hydroxypropyl tetrahydropyranotriol and tetrahydromethylpyrimidine carboxylic acid need to be pre-mixed, dispersed and dissolved in water before being added to the aqueous reaction vessel.

[0039] Specifically, 1,3-Butanediol provides an anhydrous, highly humidified, and low-viscosity environment. Pre-mixing and dissolving sodium hyaluronate and 1,3-butanediol ensures that sodium hyaluronate is completely wetted and dispersed into uniform swollen particles in a nearly anhydrous environment. This allows for rapid and complete dissolution without clumping when added to the aqueous reactor, preventing sodium hyaluronate from agglomerating into gel clusters. In contrast, directly adding vitamin C ethyl ether, acetylglucosamine, or tetrahydromethylpyrimidine carboxylic acid can easily lead to agglomeration. Furthermore, directly adding hydroxypropyl tetrahydropyranotriol can cause localized exothermic reactions, and localized overheating can affect the active ingredients, resulting in activity loss. Therefore, pre-dispersing the above raw materials ensures that each component is uniformly dispersed, without residue or degradation, thus improving preparation efficiency.

[0040] Preferably, in step S1, the homogenization rate of 1,3-butanediol and sodium hyaluronate, which are pre-mixed and dispersed at 80-85°C, is 2500-3000 r / min, and the homogenization time is 1-3 min.

[0041] Specifically, at this homogenization rate and time, to ensure uniform mixing, sodium hyaluronate and 1,3-butanediol are completely dispersed in deionized water.

[0042] The technical solution of the present invention will be further illustrated below through specific embodiments.

[0043] Example group Example 1 A dual-layer essence with anti-aging effects, the dual-layer essence being composed of an aqueous phase layer and an oil phase layer; The raw materials for preparing the aqueous phase layer, calculated by mass percentage, include 8% hydroxypropyl tetrahydropyranotriol, 0.1% tetrahydromethylpyrimidine carboxylic acid, 0.1% kava pepper extract, 5% Lactobacillus / soybean fermentation product filtrate, 1% vitamin C ethyl ether, 1% acetylglucosamine, 8% 1,3-butanediol, 0.01% sodium hyaluronate, 0.5% citric acid / sodium citrate buffer system, 10% glycerol, and the balance being deionized water. The raw materials for preparing the oil phase layer include 1.5% ascorbic acid tetraisopalmitate, 15% plant squalane, 3% jojoba seed oil, 2% camellia seed oil, 3% sunflower seed oil, 3% wild soybean oil, 0.1% meadowfoam seed oil, 1% milk thistle seed oil, 0.1% macadamia seed oil, and 0.3% tocopheryl acetate. S1. Aqueous phase preparation: 1,3-Butanediol and sodium hyaluronate were pre-mixed and dispersed at 85℃ and then added to an aqueous phase reactor containing water. After stirring until uniformly dissolved, glycerol and a buffer system were added, and stirring was continued until uniformly dissolved. The temperature was lowered to 45℃, and vitamin C ethyl ether, acetylglucosamine, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Lactobacillus / soybean milk fermentation product filtrate and kava pepper extract were added to the aqueous phase reactor. The mixture was stirred and dissolved uniformly, and the temperature was lowered to 35℃ and filtered to obtain an aqueous phase mixture. S2. Oil phase preparation: At room temperature, plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil are sequentially added into a dry oil phase reaction vessel, stirred and dissolved evenly, and filtered to obtain an oil phase mixture. S3. Mixed filling: The mass ratio of the aqueous phase layer to the oil phase layer is 4:1. The oil phase mixture is slowly added to the aqueous phase mixture, stirred evenly, and then filled to obtain a double-layer essence.

[0044] Example 2 A dual-layer essence with anti-aging effects, the dual-layer essence being composed of an aqueous phase layer and an oil phase layer; The raw materials for preparing the aqueous phase layer, calculated by mass percentage, include 3% hydroxypropyl tetrahydropyranotriol, 0.5% tetrahydromethylpyrimidine carboxylic acid, 0.06% kava pepper extract, 1% Lactobacillus / soybean fermentation product filtrate, 5% vitamin C ethyl ether, 3% acetylglucosamine, 3% 1,3-butanediol, 0.1% sodium hyaluronate, 0.3% citric acid / sodium citrate buffer system, 8% glycerol, and the balance being deionized water. The raw materials for preparing the oil phase layer include 0.5% ascorbic acid tetraisopalmitate, 10% plant squalane, 10% jojoba seed oil, 8% camellia seed oil, 1% sunflower seed oil, 1.5% wild soybean oil, 1% meadowfoam seed oil, 0.1% milk thistle seed oil, 1% macadamia seed oil, and 0.15% tocopheryl acetate. S1. Aqueous phase preparation: 1,3-Butanediol and sodium hyaluronate were pre-mixed and dispersed at 85℃ and then added to an aqueous phase reactor containing water. After stirring until uniformly dissolved, glycerol and a buffer system were added, and stirring was continued until uniformly dissolved. The temperature was lowered to 45℃, and vitamin C ethyl ether, acetylglucosamine, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Lactobacillus / soybean milk fermentation product filtrate and kava pepper extract were added to the aqueous phase reactor. The mixture was stirred and dissolved uniformly, and the temperature was lowered to 35℃ and filtered to obtain an aqueous phase mixture. S2. Oil phase preparation: At room temperature, plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil are sequentially added into a dry oil phase reaction vessel, stirred and dissolved evenly, and filtered to obtain an oil phase mixture. S3. Mixed filling: The mass ratio of the aqueous phase layer to the oil phase layer is 4:1. The oil phase mixture is slowly added to the aqueous phase mixture, stirred evenly, and then filled to obtain a double-layer essence.

[0045] Example 3 A dual-layer essence with anti-aging effects, the dual-layer essence being composed of an aqueous phase layer and an oil phase layer; The raw materials for preparing the aqueous phase layer, calculated by mass percentage, include 5% hydroxypropyl tetrahydropyranotriol, 0.3% tetrahydromethylpyrimidine carboxylic acid, 0.01% kava pepper extract, 3% Lactobacillus / soybean fermentation product filtrate, 2% vitamin C ethyl ether, 5% acetylglucosamine, 5% 1,3-butanediol, 0.08% sodium hyaluronate, 0.1% citric acid / sodium citrate buffer system, 5% glycerol, and the balance being deionized water. The raw materials for preparing the oil phase layer include 2% ascorbic acid tetraisopalmitate, 5% plant squalane, 8% jojoba seed oil, 6% camellia seed oil, 5% sunflower seed oil, 0.5% wild soybean oil, 0.6% meadowfoam seed oil, 0.5% milk thistle seed oil, 0.5% macadamia seed oil, and 0.05% tocopheryl acetate. S1. Aqueous phase preparation: 1,3-Butanediol and sodium hyaluronate were pre-mixed and dispersed at 85℃ and then added to an aqueous phase reactor containing water. After stirring until uniformly dissolved, glycerol and a buffer system were added, and stirring was continued until uniformly dissolved. The temperature was lowered to 45℃, and vitamin C ethyl ether, acetylglucosamine, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, Lactobacillus / soybean milk fermentation product filtrate and kava pepper extract were added to the aqueous phase reactor. The mixture was stirred and dissolved uniformly, and the temperature was lowered to 35℃ and filtered to obtain an aqueous phase mixture. S2. Oil phase preparation: At room temperature, plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil are sequentially added into a dry oil phase reaction vessel, stirred and dissolved evenly, and filtered to obtain an oil phase mixture. S3. Mixed filling: The mass ratio of the aqueous phase layer to the oil phase layer is 4:1. The oil phase mixture is slowly added to the aqueous phase mixture, stirred evenly, and then filled to obtain a double-layer essence.

[0046] Comparative group Comparative Example 1 Compared with Example 1, Comparative Example 1 differs in that the raw materials do not contain hydroxypropyltetrahydropyranotriol.

[0047] Comparative Example 2 Compared with Example 1, Comparative Example 2 differs in that the raw materials do not contain ascorbic acid tetraisopalmitate.

[0048] Comparative Example 3 Compared with Example 1, Comparative Example 3 differs in that the raw materials do not contain tetrahydromethylpyrimidine carboxylic acid.

[0049] Comparative Example 4 Compared with Example 1, Comparative Example 4 differs in that the raw materials do not contain lactobacillus / soy milk fermentation product filtrate.

[0050] Comparative Example 5 Compared with Example 1, Comparative Example 5 differs in that it does not contain vitamin C ethyl ether in its raw materials.

[0051] Comparative Example 6 Compared with Example 1, Comparative Example 6 differs in that the raw materials do not contain acetylglucosamine.

[0052] Comparative Example 7 Compared with Example 1, Comparative Example 7 differs in that it does not contain 1,3-butanediol and sodium hyaluronate in its raw materials.

[0053] Comparative Example 8 Compared with Example 1, the difference in Comparative Example 8 is that, during preparation, the corresponding proportions of plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil were directly added to the aqueous phase mixture in sequence, homogenized at low speed, and then filled.

[0054] The performance and application tests were performed on the two-layer essences obtained from the above-described example group and comparative example group.

[0055] Volunteers aged 35-50 years with good health and no history of skincare allergies were selected and divided into 12 groups of 10 each. One group served as a control group and did not use any products. Three groups used the double-layer essence described in Examples 1-3 after cleansing morning and evening, while eight groups used the double-layer essence described in Comparative Examples 1-8 after cleansing morning and evening. The method of use was to shake the product and then apply it to the skin. On day 28 (D28), the volunteers' facial skin was tested to measure skin elasticity, moisture content, skin tone brightness, and wrinkle appearance after using the double-layer essence. The improvement was recorded accordingly, and the test results are shown in Table 1.

[0056] Table 1

[0057] Skin irritation tests were conducted on Examples 1-3 and Comparative Examples 1-8: In accordance with the Cosmetic Safety Technical Specifications (2015 edition), 110 subjects were divided into eight groups of 10 each and a patch test was performed. The results showed no adverse reactions such as water spots or redness and swelling, and the sensitization rate was 0%, indicating that the formula is relatively safe and reliable.

[0058] Furthermore, stability tests were conducted on Examples 1-3 and Comparative Examples 1-8. Specifically, the obtained products were stored at 40°C for 30 days, and the pH value and active ingredient retention rate of the products after 30 days were tested. The results are shown in Table 2. Table 2

[0059] As shown in Table 1, Examples 1-3 can effectively improve skin tone brightness by 12.6-13.5%, increase skin moisture content (moisture content improvement rate of 35.2-35.8%), reduce wrinkles (wrinkle improvement rate of 15.8-16.3%), increase skin firmness (skin elasticity perception rate of 18.7-19.2%), and significantly reduce the burning sensation after using Examples 1-3. As shown in Table 3, Examples 1-3 have high stability; the active ingredients are stable and effective, and the bilayer structure remains stable.

[0060] Compared to Example 1, Comparative Example 1 did not contain hydroxypropyl tetrahydropyranotriol in its raw materials; compared to Example 1, Comparative Example 2 did not contain ascorbate tetraisopalmitate in its raw materials. Hydroxypropyl tetrahydropyranotriol and ascorbate tetraisopalmitate can inhibit collagen degradation, improve skin firmness, and achieve anti-wrinkle effects. Comparative Examples 1 and 2 lacked these two raw materials. After 28 days of use, the improvement rates of skin elasticity and wrinkles were lower than those in the Example 1 group, specifically, the skin improvement rate was 17.8-18.5%, lower than the 19.2% of Example 1. The wrinkle improvement rate was 14.7-14.9%, lower than the 16.3% of Example 1. This indicates that the lack of hydroxypropyl tetrahydropyranotriol and ascorbate tetraisopalmitate resulted in inferior anti-wrinkle and firming effects compared to Example 1. Furthermore, as seen in Comparative Examples 1 and 2, the presence of hydroxypropyl tetrahydropyranotriol or ascorbate tetraisopalmitate alone did not produce as good a firming and anti-wrinkle effect as the synergistic effect of these two components in Example 1. In other words, the synergistic effect of hydroxypropyl tetrahydropyranotriol or ascorbate tetraisopalmitate achieved a better improvement rate in skin elasticity and wrinkle reduction.

[0061] Compared with Example 1, Comparative Example 3 did not contain tetrahydromethylpyrimidine carboxylic acid in its raw materials, and Comparative Example 4 did not contain Lactobacillus / soy milk fermentation product filtrate in its raw materials. That is, Comparative Example 3 and Comparative Example 4 each contained tetrahydromethylpyrimidine carboxylic acid or Lactobacillus / soy milk fermentation product filtrate, respectively. It can be seen that compared with the blank example, Comparative Example 3 and Comparative Example 4 have a certain moisturizing effect. However, the improvement rate of moisture content is not as good as that of Example 1, indicating that the moisturizing effect is not as good as that of Example 1, that is, not as good as the synergistic effect of tetrahydromethylpyrimidine carboxylic acid and Lactobacillus / soy milk fermentation product filtrate.

[0062] Compared with Example 1, Comparative Examples 5, 6 and 7 do not possess the stable system formed by vitamin C ethyl ether, acetylglucosamine, 1,3-butanediol and sodium hyaluronate. As can be seen from Table 2, after being stored at 40°C for 30 days, the retention rate of active ingredients in Comparative Examples 5-7 is lower than that in Example 1.

[0063] Compared with Example 1, Comparative Example 8 did not separate the aqueous phase and oil phase during the preparation process. Instead, the oil phase components were directly added to the aqueous phase mixture. This made it difficult to balance the stability and transdermal absorption efficiency of both water-soluble and oil-soluble active ingredients, affecting the appearance and skin feel of the double-layer essence. As shown in Table 2, the active ingredient retention rate of Comparative Example 8 was 92%, which was lower than the 96% of Example 1.

[0064] Furthermore, as shown in Table 2, the pH values ​​of both the example group and the comparative group are within the range of 5.0-6.0, which effectively prolongs the effective time of the active ingredients, is close to the natural acid-base environment of the skin, and can reduce the risk of skin irritation due to pH value, making it relatively mild.

[0065] In summary, this invention utilizes hydroxypropyl tetrahydropyranotriol, ascorbic acid tetraisopalmitate, tetrahydromethylpyrimidine carboxylic acid, and Lactobacillus / soy milk fermentation product filtrate to cover three major anti-aging pathways: anti-wrinkle, anti-oxidation, and repair, achieving multi-dimensional synergistic anti-aging. Furthermore, it includes vitamin C ethyl ether, acetylglucosamine, 1,3-butanediol, and sodium hyaluronate. Through synergistic interfacial activity, stable dispersion is achieved after mixing the oil and aqueous phases, ensuring the retention rate of active ingredients and facilitating efficient penetration of subsequent active ingredients into the skin.

[0066] The technical principles of the present invention have been described above with reference to specific embodiments. These descriptions are merely for explaining the principles of the invention and should not be construed as limiting the scope of protection of the invention in any way. Based on this explanation, those skilled in the art can readily conceive of other specific embodiments of the invention without inventive effort, and these embodiments will all fall within the scope of protection of the present invention.

Claims

1. A dual-layer essence with anti-aging effects, characterized in that: The dual-layer essence consists of an aqueous phase layer and an oil phase layer; The raw materials for preparing the aqueous phase layer, calculated by mass percentage, include 3-8% hydroxypropyl tetrahydropyranotriol, 0.1-0.5% tetrahydromethylpyrimidine carboxylic acid, 0.01-0.1% kava pepper extract, 1-5% lactobacillus / soybean fermentation product filtrate, 1-5% vitamin C ethyl ether, 1-5% acetylglucosamine, 3-8% 1,3-butanediol, 0.01-0.1% sodium hyaluronate, 0.1-0.5% buffer system, 5-10% glycerol, and the balance deionized water. The raw materials for preparing the oil phase include 0.5-2% ascorbic acid tetraisopalmitate, 11-44% compounded plant-derived oils, and 0.05-0.3% tocopheryl acetate.

2. The double-layer essence with anti-aging effects according to claim 1, characterized in that: Based on the percentage by mass of the total raw materials, the compounded plant-derived oils include 5-15% plant squalane, 3-10% jojoba seed oil, 2-8% camellia seed oil, 1-5% sunflower seed oil, 0.5-3% wild soybean oil, 0.1-1% meadowfoam seed oil, 0.1-1% milk thistle seed oil, and 0.1-1% macadamia seed oil.

3. The double-layer essence with anti-aging effects according to claim 2, characterized in that: The buffer system is a citric acid / sodium citrate buffer system, with a molar ratio of citric acid to sodium citrate of 1:(1.5-2.5).

4. The double-layer essence with anti-aging effects according to claim 3, characterized in that: The mass ratio of the aqueous phase layer to the oil phase layer is (2-4):

1.

5. A double-layer essence with anti-aging effects according to claim 4, characterized in that: Sodium hyaluronate contains both high-molecular-weight sodium hyaluronate and low-molecular-weight sodium hyaluronate, with a mass ratio of (1-1.5):

1.

6. A method for preparing a double-layered essence with anti-aging effects according to any one of claims 2-5, characterized in that, Includes the following steps: S1. Aqueous phase preparation: At 80-85℃, 1,3-butanediol and sodium hyaluronate are added to an aqueous phase reactor containing water and stirred until dissolved. Then, glycerol and a buffer system are added, and stirring is continued until dissolved. The temperature is lowered to 40-45℃, and vitamin C ethyl ether, acetyl glucosamine, hydroxypropyl tetrahydropyranotriol, tetrahydromethylpyrimidine carboxylic acid, lactobacillus / soy milk fermentation product filtrate, and kava pepper extract are added to the aqueous phase reactor and stirred until dissolved. The temperature is lowered to 35℃ and filtered to obtain an aqueous phase mixture. S2. Oil phase preparation: At room temperature, plant squalane, jojoba seed oil, camellia seed oil, sunflower seed oil, wild soybean oil, meadowfoam seed oil, ascorbate tetraisopalmitate, tocopheryl acetate, milk thistle seed oil and macadamia seed oil are added into a dry oil phase reaction vessel, stirred and dissolved evenly, and filtered to obtain an oil phase mixture. S3. Mixing and filling: Slowly add the oil phase mixture to the aqueous phase mixture, stir evenly, and then fill to obtain a double-layer essence.

7. The method for preparing a double-layer essence with anti-aging effects according to claim 6, characterized in that: In step S1, sodium hyaluronate and 1,3-butanediol need to be pre-mixed, dispersed and dissolved before being added to the aqueous reaction vessel; Vitamin C ethyl ether, acetylglucosamine, hydroxypropyl tetrahydropyranotriol and tetrahydromethylpyrimidine carboxylic acid need to be pre-mixed, dispersed and dissolved in water before being added to the aqueous reaction vessel.

8. The method for preparing a double-layer essence with anti-aging effects according to claim 7, characterized in that: In step S1, 1,3-butanediol and sodium hyaluronate are pre-mixed and dispersed at 80-85℃ and then added to an aqueous phase reactor containing water. The homogenization rate is 2500-3000 r / min and the homogenization time is 1-3 min.

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