Tightening composition and application thereof

Abstract

The invention relates to the field of skin care products, in particular to a tightening composition and application thereof. The firming composition is prepared from the following raw materials: folium artemisiae argyi extract, radix puerariae extract, bidens tripartita flower / leaf / stem extract and patrinia villosa juss extract. The plant extracts in the firming composition play a synergistic effect, and the combined application of the plant extracts has the anti-aging effects of promoting collagen synthesis and scavenging free radicals. Meanwhile, the invention further discloses application of the tightening composition in skin care products.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of skin care products, in particular to a firming composition and application thereof. BACKGROUND

[0002] With the increasing demand for skin care, skin firming and anti-aging has become an important research direction in the field of cosmetics. In the prior art, most firming skin care products mainly rely on single active ingredients or simple compounding, and their effects are often limited due to the limitations of the ingredients themselves, and it is difficult to achieve significant synergistic effect. For example, some formulations can temporarily improve skin elasticity, but cannot promote collagen synthesis or effectively remove free radicals from the root, resulting in difficulty in maintaining anti-aging effect; some other schemes have antagonistic effects between ingredients, which weaken the overall efficacy. In addition, the compositions in the prior art still have room for improvement in safety, stability and compatibility, and it is urgent to develop a new composition that can systematically improve skin firmness. SUMMARY

[0003] The present application aims to solve one of the technical problems in the prior art. To this end, the present application provides a firming composition, which has good firming effect.

[0004] The present application also provides a preparation method of the firming composition.

[0005] The present application also provides application of the firming composition in preparing skin care products with firming effect.

[0006] The present application also provides a skin care product comprising the firming composition.

[0007] In the first aspect, the present application provides a firming composition, which comprises the following raw materials: extract of folium artemisiae argyi, extract of puerariae lobata, extract of flower / leaf / stem of wolf's-melon, extract of patrinia villosa juss.

[0008] The firming and anti-wrinkle composition provided by the present application exhibits a significant enhancement effect in promoting collagen COL-1 gene expression and antioxidant through the synergistic compounding of extract of folium artemisiae argyi, extract of puerariae lobata, extract of flower / leaf / stem of wolf's-melon and extract of patrinia villosa juss.

[0009] Preferably, the firming composition comprises the following mass parts of raw materials: extract of folium artemisiae argyi: 2-6 parts; extract of puerariae lobata: 2-5 parts; extract of flower / leaf / stem of wolf's-melon: 6-10 parts; extract of patrinia villosa juss: 8-12 parts.

[0010] Further preferably, the firming composition comprises the following mass parts of raw materials: Artemisia argyi extract: 3-5 parts; Pueraria lobata extract: 2-4 parts; Eupatorium fortunei flower / leaf / stem extract: 7-9 parts; Patrinia villosa extract: 9-11 parts.

[0011] Most preferably, the firming composition comprises the following parts by mass of raw materials: Artemisia argyi extract: 4 parts; Pueraria lobata extract: 3 parts; Eupatorium fortunei flower / leaf / stem extract: 8 parts; Patrinia villosa extract: 10 parts.

[0012] Further, the preparation method of the firming composition comprises the following steps: Mixing the raw materials evenly to obtain the firming composition.

[0013] In a second aspect, the present application provides the use of the firming composition of the first aspect in the preparation of a skin care product with firming efficacy.

[0014] In a third aspect, the present application provides a skin care product with firming efficacy, wherein the skin care product comprises the firming composition of the first aspect.

[0015] Preferably, the mass percentage content of the firming composition of the first aspect in the skin care product is 0.1-10%.

[0016] Preferably, the skin care product further comprises an additive.

[0017] Preferably, the additive comprises at least one of a surfactant, a thickening agent, a conditioning agent, a humectant, a preservative, a chelating agent, a fragrance, and a solvent.

[0018] Preferably, the dosage form of the skin care product is an emulsion, a cream, a powder, a block, or an oil.

[0019] Advantages of the present application: The firming composition provided by the present application realizes significant synergistic effect through scientific compounding of specific plant extracts. The composition can effectively promote the expression of skin collagen genes, enhance the synthesis of collagen, and thus enhance the elasticity and firmness of the skin, while removing free radicals to reduce oxidative damage, thereby delaying the aging process of the skin. The advantages are: High-efficiency synergistic effect: the extracts of Artemisia argyi, Pueraria lobata, Eupatorium fortunei, and Patrinia villosa form a complementary mechanism at the molecular level, significantly improving skin cell activity and strengthening skin barrier function; Natural safety: pure plant-derived ingredients are used, avoiding potential irritation of chemical synthetic substances, and being suitable for sensitive skin; Multi-dimensional anti-aging: It takes into account both collagen synthesis and free radical scavenging pathways, improves skin texture from the inside out, and reduces signs of aging such as fine lines and sagging; Compatibility stability: By optimizing the component ratio and process design, we ensure the long-term stability and bioavailability of the composition in complex skin care systems. Detailed Implementation

[0020] The following will describe the concept and technical effects of the present invention clearly and completely with reference to embodiments, so as to fully understand the purpose, features and effects of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are all within the scope of protection of the present invention.

[0021] Unless otherwise specified in the examples, the procedures should be performed under standard conditions or conditions recommended by the manufacturer. Reagents or instruments whose manufacturers are not specified are all commercially available products.

[0022] When a numerical range is disclosed herein, the range is considered continuous and includes the minimum and maximum values ​​of the range, as well as every value between the minimum and maximum values. Furthermore, when the range refers to integers, it includes every integer between the minimum and maximum values ​​of the range. Additionally, when multiple ranges are provided to describe a feature or characteristic, the ranges may be combined. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges to which they are incorporated.

[0023] The terms “comprising,” “including,” “having,” “containing,” or any other variations thereof, as used herein, are intended to cover non-exclusive inclusion. For example, a composition, step, method, or article that includes the listed elements is not necessarily limited to those elements, but may include other elements not expressly listed or elements inherent to such composition, step, method, or article.

[0024] The conjunction "composed of" excludes any unspecified elements, steps, or components. If used in a claim, this phrase makes the claim closed, excluding materials other than those described, except for associated conventional impurities. When the phrase "composed of" appears in a clause of the body of a claim rather than immediately following it, it limits only the elements described in that clause; other elements are not excluded from the claim as a whole.

[0025] "Parts by mass" refers to the basic unit of measurement that expresses the mass ratio of multiple components. One part can represent any unit mass, such as 1g or 4.435g. If we say that component A has "a" parts by mass and component B has "b" parts by mass, it means the ratio of the mass of component A to the mass of component B is a:b. Alternatively, it can mean that the mass of component A is aK and the mass of component B is bK (K is any number representing a multiplier). It is important to understand that, unlike parts by mass, the sum of the mass parts of all components is not limited to 100 parts.

[0026] Unless otherwise specified, "room temperature" in this invention means (25±2)℃.

[0027] Some of the raw materials and their sources in this invention; Artemisia argyi extract: purchased from Shaanxi Shutu Biotechnology Co., Ltd.; Kudzu root extract: purchased from Shaanxi Shutu Biotechnology Co., Ltd.; Wolfsbane flower / leaf / stem extract: purchased from Shaanxi Shutu Biotechnology Co., Ltd.; White Flower Patrinia Extract: Purchased from Shaanxi Shutu Biotechnology Co., Ltd.; All other raw materials can be purchased commercially.

[0028] The raw materials, their mass fractions, and the preparation method of the firming composition of the present invention are as follows: Composition 1: Artemisia argyi extract: 4 parts; Kudzu root extract: 3 parts; Wolfsbane flower / leaf / stem extract: 8 parts; White Flower Patrinia Extract: 10 parts; Preparation method: Mix all raw materials evenly at room temperature to obtain the firming composition.

[0029] Composition 2: Artemisia argyi extract: 6 parts; Kudzu root extract: 5 parts; Wolfsbane flower / leaf / stem extract: 10 parts; White Flower Patrinia Extract: 12 parts; The preparation method is the same as that of composition 1.

[0030] Composition 3: Artemisia argyi extract: 2 parts; Kudzu root extract: 2 parts; Wolfsbane flower / leaf / stem extract: 6 parts; White Flower Patrinia Extract: 8 parts; The preparation method is the same as that of composition 1.

[0031] Composition ①: Unlike composition 1, it lacks Artemisia argyi extract. The missing mass parts are made up by Pueraria lobata extract, Lysimachia christinae flower / leaf / stem extract and Patrinia scabiosifolia extract in a mass ratio of 3:8:10. The preparation method is the same as that of composition 1.

[0032] Composition ②: Unlike composition 1, it lacks kudzu root extract. The missing mass parts are made up by the extracts of Artemisia argyi, Clematis chinensis flower / leaf / stem extract and Patrinia scabiosifolia extract in a mass ratio of 4:8:10. The preparation method is the same as that of composition 1.

[0033] Composition ③: Unlike Composition 1, it lacks the extract of wolfberry flower / leaf / stem. The missing mass parts are made up by the extracts of Artemisia argyi, Pueraria lobata, and Patrinia scabiosifolia in a mass ratio of 4:3:10. The preparation method is the same as that of Composition 1.

[0034] Composition ④: Unlike Composition 1, it lacks the extract of Patrinia scabiosifolia. The missing mass parts are made up by the extracts of Artemisia argyi, Pueraria lobata, and Clematis armandii flower / leaf / stem in a mass ratio of 4:3:8. The preparation method is the same as that of Composition 1.

[0035] Composition ⑤: Unlike Composition 1, the extract of Patrinia scabiosifolia is replaced with Patrinia scabiosifolia extract of equal mass, while the other raw material parameters and preparation methods are the same as those of Composition 1.

[0036] Composition ⑥: Artemisia argyi extract: 8 parts; Kudzu root extract: 10 parts; Wolfsbane flower / leaf / stem extract: 4 parts; White Flower Patrinia Extract: 3 parts; The preparation method is the same as that of composition 1.

[0037] The components, their mass percentages, and the preparation method of the skincare product with firming effects described in this invention are as follows: Skincare product 1: Composition 1: 5%; Moisturizer: 10%; Preservative: 0.1%; Thickener: 0.5%; Surfactant: 0.5%; Replenish solvent to 100%; Preparation method: (1) The humectant and preservative raw materials and 1 / 4 solvent were stirred evenly at 63°C, and then cooled to 40°C to obtain phase A; (2) Stir the thickener, surfactant raw materials and 1 / 4 solvent evenly to obtain phase B; (3) Mix the composition with the remaining solvent, stir until homogeneous, then add phase A and phase B, and stir until homogeneous; (4) After passing the inspection, the material can be filtered through a 300-mesh sieve.

[0038] Skincare Product 2: Unlike Skincare Product 1, it uses Composition 2 to replace Composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of Skincare Product 1.

[0039] Skincare Product 3: Unlike Skincare Product 1, it uses Composition 3 to replace Composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of Skincare Product 1.

[0040] Skin care product ①: Unlike skin care product 1, composition ① is used to replace composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of skin care product 1.

[0041] Skin care product ②: Unlike skin care product 1, composition ② is used to replace composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of skin care product 1.

[0042] Skin care product ③: Unlike skin care product 1, composition ③ is used to replace composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of skin care product 1.

[0043] Skin care product ④: Unlike skin care product 1, composition ④ is used to replace composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of skin care product 1.

[0044] Skin care product ⑤: Unlike skin care product 1, composition ⑤ is used to replace composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of skin care product 1.

[0045] Skin care product ⑥: Unlike skin care product 1, composition ⑥ is used to replace composition 1 in equal amounts, while the remaining components and their preparation methods are the same as those of skin care product 1.

[0046] Skincare Product ⑦: Unlike Skincare Product 1, it lacks a composition, and the missing amount is made up with a solvent. The remaining components and their preparation methods are the same as those of Skincare Product 1.

[0047] In the above-mentioned skin care products 1-3 and skin care products ①-⑦, the moisturizer is glycerin; the preservative is phenoxyethanol; the thickener is hydroxyethyl cellulose and carrageenan in a mass ratio of 1:1.5; the surfactant is cocamidopropyl betaine; and the solvent is deionized water.

[0048] Efficacy verification: Experiment 1: Effect of the composition on the expression level of COL-1 gene in human skin fibroblasts Type I collagen (COL-1) is mainly found in skin, bones, muscles, and other connective tissues, playing a crucial role in maintaining tissue structure integrity and elasticity. When COL-1 is specifically degraded, the structure of collagen and elastic fibers in the body is damaged, leading to signs of aging such as wrinkles. This experiment investigated the effect of compositions 1-3 and compositions ①-⑥ (samples to be tested) on the expression level of the COL-1 gene in fibroblasts. The experimental steps are as follows: The samples to be tested are compositions 1-3 and compositions ①-⑥.

[0049] Human skin fibroblasts (HSF cells) cultured to passage 3 were divided into groups of 5 × 10⁻⁶ cells. 5 Cells were seeded in 96-well plates, mixed, and incubated at 37°C and 5% CO2 for 24 h. The blank group (BC) had its culture medium replaced with serum-free cell culture medium; the control group (NC) had its culture medium replaced with serum-free cell culture medium containing 200 μmol / L H2O2; and the experimental group had its culture medium replaced with serum-free cell culture medium containing 200 μmol / L H2O2 and 0.01 wt% of the test sample. Cells in each treatment group were cultured at 37°C and 5% CO2 for another 3 days, and the COL-1 gene expression level was determined by qPCR. The effect (X) on COL-1 expression in HSF cells was calculated using the following formula: X = Q x / Q0 Among them, Q x This represents the gene expression level of each group as determined by qPCR, with Q0 representing the gene expression level of the blank group (BC) as determined by qPCR.

[0050] The results are shown in Table 1.

[0051] Table 1. Effect of the composition on COL-1 gene expression in HSF cells (n=3, X±SD) Group Effect on intracellular COL-1 gene expression BC 1.00±0.01* NC 0.72±0.03* Composition 1 1.74±0.08 Composition 2 1.61±0.07 Composition 3 1.65±0.04 Composition 1 1.35±0.09* Composition 2 1.29±0.10* Composition 3 1.31±0.05* Composition 4 1.33±0.11* Composition 5 1.41±0.12* Composition 6 1.39±0.06* Note: "*" indicates that compared with composition 1, p < 0.05.

[0052] As shown in Table 1, compositions 1-3 and ①-⑥ can all increase the expression level of the COL-1 gene in fibroblasts. According to the NC group data, hydrogen peroxide can significantly reduce the expression level of the COL-1 gene in HSF cells. Comparing the results of compositions 1-3 with the NC group, it can be seen that the firming composition of the present invention can significantly increase the expression level of the COL-1 gene in HSF cells. Comparing the results of composition 1 with compositions ①-④, it can be seen that the effect of the firming composition will be worse when any ingredient in the firming composition is omitted. Comparing the results of composition 1 with composition ⑤, it can be seen that the effect of the firming composition will also be worse when using *Patrinia scabra* instead of *Patrinia nigra*. Comparing the results of composition 1 with composition ⑥, it can be seen that when *Artemisia argyi* extract, *Pueraria lobata* extract, *Lysimachia christinae* flower / leaf / stem extract, and *Patrinia nigra* extract are used together in the dosage ratio specified in the present invention, the expression level of the COL-1 gene in HSF cells can be greatly increased.

[0053] Experiment 2: Effect of the composition on the scavenging ability of superoxide anion free radicals Test samples: Compositions 1-3, Compositions ①-⑥, solvent is deionized water, concentration is 1 mg / mL.

[0054] Take 4.5 mL of 0.05 mol / L Tris-HCl buffer solution (pH = 8.2) and preheat it in a 25°C water bath for 30 min. Then add 1 mL of the sample to be tested and 0.4 mL of 25 mol / L pyrogallol aqueous solution, mix well, and react in a 25°C water bath for 5 min. Add 1 mL of 8 mol / L hydrochloric acid aqueous solution to terminate the reaction. Use Tris-HCl buffer solution as a reference and measure the absorbance at 299 nm.

[0055] The formula for calculating the superoxide anion radical scavenging rate is shown below: Superoxide anion radical scavenging rate (%) = [1 - (A2 / A1)] × 100%.

[0056] In the formula, A1 is the absorbance value of the blank control group, which is the sample to be tested replaced with an equal amount of deionized water; A2 is the absorbance value of the sample group.

[0057] The results are shown in Table 2. The results were tested 3 times and the average value was taken.

[0058] Table 2. Effect of the composition on superoxide anion radical scavenging rate (n=3) Group Superoxide anion radical scavenging rate (%) Composition 1 68.35 Composition 2 67.09 Composition 3 65.53 Composition 1 44.66 Composition 2 40.31 Composition 3 47.42 Composition 4 44.37 Composition 5 51.06 Composition 6 50.39 As shown in Table 2, compositions 1-3 and ①-⑥ all exhibit a certain scavenging ability against superoxide anion free radicals. Comparing the results of composition 1 with those of compositions ①-④, it is evident that the scavenging ability of the firming composition against superoxide anion free radicals decreases when any ingredient in the firming composition is omitted. Comparing the results of composition 1 with that of composition ⑤, it is evident that, compared to *Patrinia scabiosifolia* extract, *Patrinia scabiosifolia* extract, combined with *Artemisia argyi* extract, *Pueraria lobata* extract, and *Gnaphalium affine* flower / leaf / stem extract, has a stronger scavenging effect against superoxide anion free radicals. Comparing the results of composition 1 with that of composition ⑥, it is evident that when *Artemisia argyi* extract, *Pueraria lobata* extract, *Gnaphalium affine* flower / leaf / stem extract, and *Patrinia scabiosifolia* extract are used together in the dosage ratios specified in this invention, the scavenging rate of the composition against superoxide anion free radicals can be greatly improved.

[0059] Human efficacy trials: Test 1: Safety Test This experiment used skincare products 1-3 and ①-⑦ as test substances, and conducted according to the method of human skin patch testing in the "Cosmetic Safety Technical Specifications" (2015 edition). Three hundred volunteers aged 18-60 years who met the experimental requirements were selected as subjects (half male and half female). The test substance was added to the patch applicator at a dosage of 0.020-0.025g. The control well served as a blank control (without any substance). The patch applicator containing the test substance was applied to the flexor side of the subject's forearm using non-irritating adhesive tape, and the patch was gently pressed with the palm of the hand to ensure even application to the skin. The test site was marked for observation. The patch was applied every 24 hours. After removing the patch, the skin reaction was observed 30 minutes after the pressure mark disappeared. If a suspicious or positive reaction was observed, the patch test was discontinued for that subject; if a negative reaction was observed, the patch test was repeated on the same test site for a total of 6 repetitions. The results of the subjects' reactions were recorded according to the skin adverse reaction grading criteria in Table 3. The test results are shown in Table 4.

[0060] Table 3 Identification Criteria Grade Identification criteria 0 Negative reaction: no irritation, no erythema 1 Suspected reaction: mild erythema 2 Weak positive reaction: erythema 3 Strong positive reaction: erythema, papules, blisters 4 Extremely strong positive reaction: severe edema, Table 4. Results of skincare product safety tests (n=30) Group 1st 2nd 3rd 4th 5th 6th Skin care product 1 0 0 0 0 0 0 Skin care product 2 0 0 0 0 0 0 Skin care product 3 0 0 0 0 0 0 Skin care product 1 0 0 0 0 0 0 Skin care product 2 0 0 0 0 0 0 Skin care product 3 0 0 0 0 0 0 Skin care product 4 0 0 0 0 0 0 Skin care product 5 0 0 0 0 0 0 Skin care product 6 0 0 0 0 0 0 Skin care product 7 0 0 0 0 0 0 As shown in Table 4, skincare products 1-3 and ①-⑦ showed no adverse reactions after patch testing, indicating that skincare products 1-3 and ①-⑦ all have good gentleness and safety.

[0061] Experiment 2: Anti-wrinkle ability test This experiment tested the anti-wrinkle ability of skincare products 1-3 and skincare products ①-⑦ (samples to be tested).

[0062] Three hundred subjects (healthy, with normal skin, no history of cosmetic allergies, aged 35-45) were selected and randomly divided into 10 groups, with an equal number of men and women. The subjects used the test sample twice daily, morning and evening, at a dosage of 0.5g each time, for 28 consecutive days. On day 28, the subjects sat still for 30 minutes in a test environment with a temperature of 20±2℃ and humidity of 50±10%. The total area of ​​wrinkles was measured using VISIA imaging to comprehensively evaluate the anti-wrinkle efficacy of the test sample on human skin.

[0063] Change rate of total wrinkle area: This refers to the rate of change of the total wrinkle area after using the test sample relative to before use. A positive value indicates an increase in the total wrinkle area (more wrinkles), while a negative value indicates a decrease in the total wrinkle area (less wrinkles).

[0064] The results are shown in Table 5, and the average value of the results is taken.

[0065] Table 5. Effects of skincare products on the area of ​​facial wrinkles (n=30) Group Change rate of total wrinkle area D28 (%) Skin care product 1 -19.51 Skin care product 2 -18.42 Skin care product 3 -18.77 Skin care product 1 -13.55 Skin care product 2 -11.39 Skin care product 3 -12.53 Skin care product 4 -13.34 Skin care product 5 -15.27 Skin care product 6 -14.71 Skin care product 7 -2.57 As shown in Table 5, the results of skincare products 1-3, ①-⑥ and ⑦ indicate that the composition has a certain ability to improve facial wrinkles.

[0066] The embodiments described above are some, but not all, of the embodiments of this application. The detailed description of the embodiments of this application is not intended to limit the scope of the claimed application, but merely to illustrate selected embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of this application without inventive effort are within the scope of protection of this application.

Claims

1. A firming composition, characterized in that, The composition comprises the following raw materials: Artemisia argyi extract, Pueraria lobata extract, Lysimachia christinae flower / leaf / stem extract, and Patrinia scabiosifolia extract.

2. The firming composition according to claim 1, characterized in that, The composition comprises the following raw materials in parts by weight: Artemisia argyi extract: 2-6 parts; Kudzu root extract: 2-5 parts; Wolfsbane flower / leaf / stem extract: 6-10 parts; White patrinia extract: 8-12 parts.

3. The firming composition according to claim 2, characterized in that, The composition comprises the following raw materials in parts by weight: Artemisia argyi extract: 3-5 parts; Kudzu root extract: 2-4 parts; Wolfsbane flower / leaf / stem extract: 7-9 parts; White patrinia extract: 9-11 parts.

4. The firming composition according to claim 3, characterized in that, The composition comprises the following raw materials in parts by weight: Artemisia argyi extract: 4 parts; Kudzu root extract: 3 parts; Wolfsbane flower / leaf / stem extract: 8 parts; White Flower Patrinia Extract: 10 parts.

5. The use of the firming composition according to any one of claims 1-4 in the preparation of skin care products having firming effects.

6. A skincare product with firming effects, characterized in that, The skin care product includes the firming composition according to any one of claims 1-4.

7. The skincare product according to claim 6, characterized in that, The firming composition in the skin care product has a mass percentage of 0.1-10%.

8. The skincare product according to claim 6, characterized in that, The skincare products also include additives.

9. The skincare product according to claim 8, characterized in that, The additives include at least one of surfactants, thickeners, conditioning agents, humectants, preservatives, chelating agents, fragrances, and solvents.

10. The skincare product according to any one of claims 6-9, characterized in that, The skincare product dosage forms are lotions, creams, powders, blocks, or oils.