Heparin sodium efficient extraction process based on enzymolysis method

By combining high-speed crushing and low-temperature graded heating enzymatic hydrolysis with resin adsorption and gradient elution, the problem of incomplete enzymatic hydrolysis was solved, achieving efficient extraction of heparin sodium, improving yield and purity, and ensuring product quality.

CN121343030APending Publication Date: 2026-01-16TIANCHANG TIANTAI CASING FOOD CO LTD
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Patent Information

Application Number
CN202511568296.1
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-10-30
Publication Date
2026-01-16

AI Technical Summary

Technical Problem

Existing heparin sodium extraction processes involve incomplete enzymatic hydrolysis and require multiple heating cycles, resulting in high energy consumption, incomplete separation of heparin-protein complexes, and incomplete removal of impurities, which affect yield and purity. Furthermore, traditional methods may damage the bioactivity of heparin sodium.

Method used

High-speed crushing of raw materials is used to increase the enzyme contact area. Combined with low-temperature staged heating enzymatic hydrolysis, resin adsorption and gradient elution, the heparin-protein complex is fully decomposed and impurities are removed by controlling temperature and ionic strength.

Benefits of technology

This improved the yield and purity of heparin sodium, reduced production energy consumption, and ensured the bioactivity and extraction efficiency of heparin sodium.

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Abstract

The invention relates to the technical field of heparin sodium, in particular to a heparin sodium efficient extraction process based on an enzymolysis method. The method comprises the steps of raw material treatment, heating enzymolysis, cooling adsorption, elution, ethanol precipitation, dehydration drying and index determination. In the high-efficiency extraction process of the heparin sodium based on the enzymolysis method, the small intestine mucous membrane is crushed into a minced state to increase the enzyme contact area, and a low-temperature graded heating enzymolysis process is combined, so that the high-efficiency decomposition of a heparin-protein compound is realized, and the problems that the enzymolysis is not thorough and the temperature needs to be increased for multiple times in the traditional enzymolysis method are effectively solved; meanwhile, insoluble impurities are removed through filtration while the solution is hot, protein residues and impurity interference are reduced in cooperation with cooled resin adsorption and gradient elution, the yield of the heparin sodium is increased, production energy consumption and purification difficulty are reduced, and therefore the overall efficiency and economical efficiency of extraction of the heparin sodium are improved.
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