An Indel molecular marker closely associated with the soluble sugar content of pepper fruit and its application

By developing Indel molecular markers associated with the soluble sugar content of chili pepper fruits and using PCR amplification to detect DNA in chili pepper leaves, the problems of long cycles and environmental dependence in traditional breeding have been solved. This has enabled early and efficient screening of chili pepper germplasm with high soluble sugar content, improving breeding efficiency and accuracy.

CN121406823BActive Publication Date: 2026-05-05SHANDONG YONGSHENG AGRI DEV CO LTD
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Patent Information

Application Number
CN202511984801.0
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-12-26
Publication Date
2026-05-05
Estimated Expiration
2045-12-26

AI Technical Summary

Technical Problem

The lack of Indel molecular markers closely related to the soluble sugar content of pepper fruits in existing technologies leads to long breeding cycles, reliance on phenotypic selection, and susceptibility to environmental interference in traditional breeding methods, making it difficult to achieve early and accurate screening of pepper germplasm with high soluble sugar content.

Method used

We developed an Indel molecular marker closely associated with the soluble sugar content of pepper fruits. By amplifying the DNA in pepper leaves using specific primers via PCR, a 219bp product was amplified, which can predict the soluble sugar content of the fruit, enabling early and non-destructive screening of pepper germplasm with high soluble sugar content.

Benefits of technology

This method enables early, efficient, and non-destructive screening of chili germplasm with high soluble sugar content, significantly shortening the breeding cycle, improving selection efficiency, and enhancing the accuracy and cost-effectiveness of molecular marker-assisted breeding of chili peppers.

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Abstract

This invention provides an Indel molecular marker closely related to the soluble sugar content of pepper fruit and its application, belonging to the technical field of nucleic acid-based assay methods. The nucleotide sequence of the Indel molecular marker is shown in SEQ ID NO.1 of the sequence listing; the Indel molecular marker is located at positions 5274716-5274727 of the full-length sequence of chromosome 3 of the pepper reference genome. Primers for amplifying the Indel molecular marker include a forward primer and a reverse primer. The nucleotide sequence of the forward primer is shown in SEQ ID NO.2 of the sequence listing, and the nucleotide sequence of the reverse primer is shown in SEQ ID NO.3 of the sequence listing. This molecular marker can be directly applied in early generations of breeding, avoiding the destructive sampling of chemical detection, significantly reducing breeding costs, effectively shortening the breeding cycle, and greatly improving the selection efficiency of molecular marker-assisted breeding in peppers.
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