Sample cracking method and sample cracking control system

Abstract

The invention relates to the technical field of medical instruments, and provides a sample cracking method and a sample cracking control device for a sample treatment device, and the sample cracking method comprises the following steps: controlling an action part to execute a cracking process on a sample by target power; recording a real-time energy value of the acting part during the execution period of the cracking process, and calculating an accumulated energy value of the acting part during the execution period of the cracking process based on the real-time energy value; when the accumulated energy value reaches the target energy value, the cracking process of the acting part is stopped; wherein when the real-time energy value is recorded at each moment, the calculation energy value is obtained according to the real-time power of the acting part at the moment; temperature information of the sample at the moment is obtained, the calculated energy value is corrected according to the temperature information, and a real-time energy value is obtained. The cracking process of the cracking action part can be corrected in real time according to the sample temperature, so that the phenomenon of insufficient cracking caused by temperature rise in the prior art is avoided.

Description

Technical Field

[0001] This invention relates to the field of medical device technology, and in particular to a sample lysis method and a sample lysis control system. Background Technology

[0002] In the field of in vitro diagnostics, biolysis is a commonly used sample processing technique. Its core principle is to disrupt the structure of biological samples (such as cells, bacteria, and viruses) through mechanical effects, releasing internal biomolecules (such as nucleic acids, proteins, and metabolites) for subsequent detection and analysis. In existing technologies, ultrasonic destruction is a common method in the lysis reaction, typically using a fixed power or current output control. When the output power or current reaches a set range and remains there for a certain period, lysis is considered complete. However, during lysis, heat may continuously accumulate in the ultrasonic head or sample tube, causing the sample and ultrasonic head temperatures to rise. At higher temperatures, the actual effective lysis energy decreases, making it impossible to guarantee sufficient or excessive lysis within a specified time. Existing technologies do not consider the impact of temperature rise caused by ultrasonic power on the sample and sample tube. Summary of the Invention

[0003] In order to overcome the shortcomings of the prior art, the present invention aims to provide a sample lysis method and a sample lysis control system, which can make real-time corrections to the lysis process of the lysis action component based on the sample temperature, so as to avoid the phenomenon of incomplete lysis due to temperature rise in the prior art.

[0004] In a first aspect, the present invention provides a sample lysis method for a sample processing apparatus, comprising: The target power control component performs the lysis process on the sample; Record the real-time energy value of the active component during the pyrolysis process, and calculate the cumulative energy value of the active component during the pyrolysis process based on the real-time energy value; When the accumulated energy value reaches the target energy value, the decomposition process of the active component is stopped; When the real-time energy value is recorded at various times, the method further includes: The calculated energy value at that moment is obtained based on the real-time power output by the active component at that moment; The temperature information of the sample at that time is obtained, and the calculated energy value is corrected based on the temperature information at that time to obtain the real-time energy value at that time.

[0005] The sample lysis method provided by the first aspect of the present invention achieves precise control of the lysis process by accumulating the energy value during the lysis operation of the active component. This solves the problem of difficulty in grasping the lysis progress in the prior art, which simply uses a specified working time to determine the completion of lysis. Furthermore, it introduces a correction factor based on temperature information, and corrects the effective energy value output at that moment in real time when the temperature is above the safe level. By taking the temperature environment into account during the lysis process, lysis is completed when the accumulated energy value reaches the target energy value. This ensures that the active component performs sufficient lysis of the sample without incomplete lysis.

[0006] In a preferred embodiment of the present invention, when the target power control component performs the lysis process on the sample, the target power is adjusted according to the temperature information of the sample at the current moment; The target power adjustment process includes the following steps: When the active component begins to perform the lysis process on the sample, the output of the active component is controlled with a first target power; Based on the current temperature information, determine whether the sample is in a normal state or a first abnormal state; When the sample is in a normal state, the output of the active component is controlled by the first target power; When the sample is in a first abnormal state, the output of the active component is controlled with a second target power that is less than the first target power.

[0007] In a preferred embodiment of the present invention, determining whether the sample is in a normal state or a first abnormal state based on the current temperature information includes: The current temperature of the sample is obtained in real time at the current moment; If the current temperature does not exceed the first temperature threshold, the sample is determined to be in a normal state. If the current temperature is greater than the first temperature threshold and less than the second temperature threshold, the sample is determined to be in a first abnormal state. The target power adjustment process also includes: Determine whether the sample is in a second abnormal state based on the current temperature information; If the sample is in a second abnormal state, the output of the active component is stopped.

[0008] In a preferred embodiment of the present invention, the step of correcting the calculated energy value based on the temperature information within that time period includes: When the sample is in a normal state, the temperature correction value does not affect the real-time energy value obtained based on the calculated energy value; When the sample is in a first abnormal state, the calculated energy value is corrected according to the temperature correction value that changes with the current temperature of the sample to obtain the real-time energy value; When the sample is in the second abnormal state, the real-time energy value is zero.

[0009] In a preferred embodiment of the present invention, when the sample is in a first abnormal state, the temperature correction value that varies with the current temperature of the sample is obtained by the following formula. b=1-k1*(T i -T k1 ) Where b is the temperature correction value, k1 is the temperature correction coefficient, and T i T represents the current temperature of the sample. k1 Let b be the first temperature threshold, and b < 1; When the sample is in the first abnormal state, the real-time energy value is obtained by multiplying the calculated energy value and the temperature correction value.

[0010] In a preferred embodiment of the present invention, the step of correcting the calculated energy value based on the temperature information within that time period includes: The current temperature of the sample is obtained in real time at the current moment; The temperature rise of the sample at the current moment is obtained based on the current temperature of the sample at the current moment and the temperature of the sample at the previous moment; The calculated energy value is corrected based on the temperature rise value to obtain the real-time energy value.

[0011] In a preferred embodiment of the present invention, the calculated energy value is corrected based on the temperature rise value, including: When the temperature rise value does not exceed the temperature rise threshold, the calculated energy value is corrected according to the effective working time correction value that changes with the temperature rise value to obtain the real-time energy value; When the temperature rise value is greater than the temperature rise threshold, the effective working time correction value at that moment is consistent with the effective working time correction value at the start of the pyrolysis process.

[0012] In a preferred embodiment of the present invention, when the temperature rise value does not exceed the temperature rise threshold, the effective working time correction value that varies with the temperature rise value is obtained by the following formula. Di = Di-1 + k2 Where Di is the effective working time correction value at the current moment, Di-1 is the effective working time correction value at the previous moment, k2 is the temperature rise correction coefficient, D1 is the effective working time correction value at the start of the cracking process, and D1 < 1, Di ≤ 1. The real-time energy value is obtained by multiplying the calculated energy value and the effective working time correction value.

[0013] In a second aspect, the present invention also provides a sample lysis control system applied to a sample processing apparatus, comprising: An execution unit is used to control the active components of the sample lysis device to perform the lysis process on the sample; A timing unit used to record time; The acquisition unit is used to acquire the temperature information of the sample and the monitoring information of the functional component; A calculation unit is used to calculate the calculated energy value of the active component during the pyrolysis process based on the monitoring information acquired by the acquisition unit. The correction unit is used to correct the calculated energy value to a real-time energy value based on the temperature information acquired by the acquisition unit. The processing unit is used to control the execution unit to cause the sample lysis device to perform a lysis process on the sample, control the calculation unit to calculate the cumulative energy value of the active component during the lysis process based on the real-time energy value corrected by the correction unit, and control the execution unit to stop the lysis process of the active component when the cumulative energy value reaches the target energy value.

[0014] In a third aspect, the present invention further provides a computer-readable storage medium storing a computer program that, when executed by a processor, performs the sample fragmentation method as described in the first aspect embodiment.

[0015] Other features and advantages of the invention will be set forth in the following description and will be apparent in part from the description or may be learned by practicing the invention. The objects and other advantages of the invention may be realized and obtained by means of the structures and / or processes particularly pointed out in the description, claims, and drawings. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of a sample processing device provided in an embodiment of the present invention;

[0017] Figure 2 A flowchart of a sample lysis method provided in an embodiment of the present invention;

[0018] Figure 3 A flowchart illustrating the method for obtaining the cumulative energy value of a functional component according to an embodiment of the present invention;

[0019] Figure 4 A flowchart illustrating the sample lysis method provided in an embodiment of the present invention;

[0020] Figure 5 This is a schematic diagram of the sample lysis control system provided in an embodiment of the present invention. Detailed Implementation

[0021] The following detailed description of the embodiments of the present invention, in conjunction with the accompanying drawings, will provide a thorough understanding of how the present invention uses technical means to solve technical problems and achieve technical effects, enabling its implementation. It should be noted that these specific descriptions are merely intended to facilitate a clearer understanding of the present invention by those skilled in the art, and are not intended to limit the scope of the invention. For example, the terms "first" and "second" mentioned in the embodiments of the present invention are not intended to limit the invention, but are merely used to indicate the sequence numbers of multiple identical or similar devices or mechanisms. Those skilled in the art can readjust these sequence numbers for ease of description or during the organization of technical solutions. Furthermore, alternative solutions are described for some mechanisms in different embodiments, and these alternatives can be applied to other identical or similar devices or mechanisms. As long as there is no conflict, the various embodiments and features in each embodiment of the present invention can be combined with each other, and the resulting technical solutions are all within the protection scope of the present invention.

[0022] Lysis is a commonly used sample processing technique in the field of in vitro diagnostics, such as... Figure 1 As shown, the sample processing device includes a processing table 103 and an action component 102 disposed near the processing table 103. After a sample container 101 containing a biological sample is placed on the processing table 103, the action component 102 outputs mechanical energy to the sample container 101, causing the biological sample inside the sample container 101 to rupture and release the corresponding biomolecules. In common methods, the action component 102 can be implemented as an ultrasound head, which can be placed at the bottom or side of the sample container 101 to transmit ultrasound waves into the sample container 101, performing ultrasound treatment on the biological sample to lyse the corresponding biological tissue, providing conditions for subsequent diagnostic work. In the prior art, the controller of the sample processing device can control the action component 102 to output a constant power or current to the sample container 101. When the control power or current is greater, the action component 102 outputs more mechanical energy. The large amount of heat generated at this time causes the sample temperature inside the sample container 101 to rise, causing the effective output value of the action component 102 to decrease. If the temperature continues to rise, it may even lead to sample denaturation or melting of the sample container 101, posing safety risks.

[0023] Accordingly, the present invention provides a sample lysis method for a sample processing device in a first aspect embodiment, see below. Figure 2 Specifically, it includes: S100, the target power control component performs the pyrolysis process on the sample; For example, during this step, the target power P0 is used to control the action component to perform the decomposition operation. The control method for the target power P0 can be by limiting the working voltage U or working current A passing through the action component, or directly by the real-time power P fed back. i Adjust the power of the active component to the target power P0. When the active component starts to operate, the real-time voltage value (or amplitude) U passing through the active component is acquired in real time through the monitoring unit. i and real-time current value A i Therefore, the real-time power at the corresponding moment is P. i =U i *A i Then, the obtained real-time power P i Compared with the target power P0, when P i When the voltage is less than P0, the operating voltage U and / or operating current A applied to the active component are increased to make P... i Approaching P0, similarly, when P i If the voltage U is greater than P0, then the voltage U should be appropriately reduced. i and / or current A i This stabilizes the output power of the active component at the target power P0, thereby maintaining a stable output.

[0024] It should be noted that when the target power is used to control the pyrolysis process of the active component, the target power can remain constant throughout the process, or it can be adjusted under certain conditions. For example, considering the effectiveness of power output during the pyrolysis process, the target power can be adjusted in segments: one target power in the first time segment and another target power in the second time segment. Alternatively, the target power can be adaptively adjusted when monitored values ​​such as the temperature of the sample or the active component, or the cumulative output energy, reach corresponding critical conditions to ensure the orderly and safe conduct of the pyrolysis process. This target power adjustment stage can be implemented in subsequent steps, spanning the entire pyrolysis process of the active component.

[0025] In addition to the embodiments mentioned above, there may be other embodiments that achieve the same purpose, all of which are within the scope of this invention.

[0026] S200 records the real-time energy value of the active component during the pyrolysis process and calculates the cumulative energy value of the active component during the pyrolysis process based on the real-time energy value; Because the active component generates non-pyrolysis process losses during energy output, these losses can be heat or other energy losses. In this embodiment, the main consideration is the effect of temperature. The losses vary at different temperatures. When the temperature is at or below the safe temperature, the losses are relatively small, and the actual output energy value used for the pyrolysis reaction accounts for a larger proportion. However, when the temperature is above the safe temperature, the losses are large, and the effective output proportion of pyrolysis energy decreases. Generally, the higher the temperature, the smaller the effective output proportion. Therefore, under the same target power control, the less pyrolysis energy is output. Thus, this real-time energy value is the effective active energy value of the active component used for the pyrolysis process, excluding heat losses.

[0027] During the pyrolysis process, real-time energy values ​​are recorded at various points in time, and the cumulative energy value is derived from these real-time energy values. (See reference...) Figure 3 Specifically: S210, the calculated energy value at that moment is obtained based on the real-time power output by the active component at that moment; The real-time power P i This information can be obtained through a monitoring unit, and the time can be divided into a unit of time Δt based on clock information. i Therefore, the calculated energy value at that moment is Q. i =P i *△t i (i∈1,2,...,n), in one embodiment, Δt per unit time i The duration is the same, P i The real-time power on the active component at that moment, and the real-time voltage U obtained by the monitoring unit at that moment. i and real-time current A i get.

[0028] S220: Obtain the temperature information of the sample at that moment and correct the calculated energy value based on the temperature information at that moment to obtain the real-time energy value at that moment.

[0029] Because temperature variations can lead to differences in the actual output energy of the active component, especially when the temperature exceeds the safe limit, the actual output energy exerted by the active component on the sample container deviates significantly from the theoretical output energy. Therefore, this embodiment introduces a temperature correction factor to adjust the calculated energy value based on temperature information, making it closer to the actual output energy value. Referring to the aforementioned embodiment, let's assume the temperature correction factor is f(T). i Let each moment be defined as a unit of time, and this unit of time be 1. The unit corresponding to this unit of time can be set to 1 second, 0.1 seconds, or other values. Then, the real-time energy q at that moment is calculated. i To calculate the energy value Q i With temperature correction factor f(T)i The product between ) is the real-time energy value q. i =f(T i )*Q i (i∈1,2,...,n). Of course, besides using the correction method of multiplying the temperature correction factor by the calculated energy value, other theoretical or empirical functions can also be used to obtain the real-time energy value at that moment, all of which are within the scope of this invention. It should also be understood that the temperature information used to correct the calculated energy value in this embodiment does not mean that the calculated energy value must be changed at every moment. For example, when the temperature T does not exceed the safe temperature K, i.e., T≤K, according to the principles of physics, most of the output energy of the active component is output as ultrasound to the sample container. Therefore, the influence of temperature on the active component can be ignored at this time, and the calculated energy value can be considered as the actual energy value. However, when the temperature T exceeds the safe temperature K, i.e., T>K, it indicates that the temperature has a greater influence on the active component, and it is necessary to further introduce a temperature correction factor or other functions to correct the calculated energy value to the actual energy value. Therefore, the correction of the calculated energy value to the real-time energy value based on temperature mentioned in this invention can be a correction under set conditions. Wherein, the temperature correction factor f(T) i The value can be an empirical value obtained from experimental data at the corresponding temperature, or a correlation value of an empirical function curve obtained based on empirical values ​​and / or theoretical conditions at the corresponding temperature.

[0030] After obtaining the real-time energy values ​​at each moment in steps S210 and S220, the cumulative energy value can be obtained by accumulating the implemented energy values ​​at each moment. Assuming the cumulative energy value is Q, the initial moment Δt1 is taken as the start time when the active component begins to execute the pyrolysis process. Then, at each moment during the pyrolysis process, a corresponding actual energy value q will be generated. i Under the condition of ensuring the output of the active component, the cumulative energy value Q continuously increases as the pyrolysis process proceeds, that is... It can serve as a feedback indicator of the lysis process. When the energy actually input to the sample container by the active component is sufficient, the biological sample can be fully lysed and the corresponding biomolecules can be completely released.

[0031] S300: When the accumulated energy value reaches the target energy value, the decomposition process of the active component is stopped.

[0032] The target energy value is set to Q. k The target energy value Q k The target energy value is the effective pyrolysis energy threshold, or an empirical value above the effective pyrolysis energy threshold. It can be reasonably and comprehensively set based on factors such as the sample size of the sample container, the power of the active component, the sample type, and the error range. When the continuously accumulating energy value Q reaches the target energy value Q...k In this case, it proves that the pyrolysis process has been completed, and the output of the working component is stopped.

[0033] Existing technologies typically use time control to determine whether lysis has been sufficient. For example, after the control component starts working until a specified time t0 is reached, the temperature inside the sample container becomes uncontrollable due to the continuous accumulation of heat and environmental influences. The effective output of lysis varies at different temperatures. If the real-time effective output value of the control component at a low temperature is q... k1 The real-time effective output value of the active component at high temperatures is greater than q. k1 low q k2 Furthermore, during the continuous execution of the pyrolysis reaction, it is impossible to predict at what moment the sample temperature or the output temperature of the active component will reach the corresponding temperature value. If the temperature remains high throughout the pyrolysis reaction, the time required for the active component, operating with a low real-time effective output value, to achieve sufficient pyrolysis may be longer than the specified time t0. Using time as the criterion for determining sufficient pyrolysis is not ideal, as it fails to account for the influence of temperature on the pyrolysis process. Compared to existing technologies, the cumulative energy value method used in this embodiment of the invention can effectively adapt to the pyrolysis progress of the active component. For example, at low temperatures, the output of the active component is at a high level, and the time required for the cumulative energy value to reach the target energy value is shorter, thus adaptively shortening the working time of the active component. Conversely, at high temperatures, the output of the active component is at a low level, and the time required for the cumulative energy value to reach the target energy value is longer, thus adaptively extending the working time of the active component. Therefore, regardless of the sample temperature conditions under which the active component operates, the working time of the active component can be adjusted to ensure sufficient pyrolysis, and the pyrolysis process can be reflected in real time, making the overall pyrolysis operation more controllable.

[0034] In one embodiment, step S100 adjusts the target power based on the sample's current temperature information when the active component performs the lysis process on the sample with the target power control. This temperature information can be obtained by a temperature sensor monitoring the sample temperature, sample container temperature, or the output temperature of the active component. In this embodiment, the temperature information is the sample temperature. To reduce the impact of temperature on the effective output of the active component and to prevent excessively high temperatures from causing denaturation of the biological sample or even melting, it is necessary to control the temperature influence during the lysis process and appropriately adjust the sample temperature when abnormal temperature phenomena occur.

[0035] In practice, the target power adjustment process includes the following steps: S110, when the action component begins to perform the lysis process on the sample, the output of the action component is controlled with the first target power; The first target power Pk1 This is the target power P used to control the output of the active component in the initial state. Under normal circumstances, the sample temperature is at or below the safe temperature when the active component starts the pyrolysis process. To ensure the effective output of the active component is maximized, this first target power P... k1 It can be set to the optimal target power that matches the sample type, sample volume and sample container at a safe temperature. Of course, it can also be set to other target power according to the usage conditions, without too many restrictions.

[0036] S120, determine whether the sample is in a normal state or a first abnormal state based on the temperature information at the current moment; This temperature information is acquired in real time by a temperature sensor. In this embodiment, the temperature sensor obtains the real-time temperature information T of the sample based on the detection signal. i Then, the real-time temperature information T i Feedback is sent to the system used to execute this method, where the system receives real-time temperature information T. i Then, the target power output of the control component is adjusted to make the output of the control component more efficient, or the influence of temperature on the output of the control component is reduced by decreasing the power of the control component. The specific adjustment process is as follows: S121, real-time acquisition of the current temperature of the sample at the current moment; S122, if the current temperature does not exceed the first temperature threshold, the sample is determined to be in a normal state; S123, if the current temperature is greater than the first temperature threshold and less than the second temperature threshold, the sample is determined to be in the first abnormal state.

[0037] In this embodiment, the normal state of the sample refers to the sample temperature being at or below the safe temperature. The first abnormal state refers to the sample temperature being at the warning temperature but not exceeding the danger temperature. It is assumed that the safe temperature threshold, i.e., the first temperature threshold, is T. k1 And the danger temperature, i.e., the second temperature threshold, is T. k2 The warning temperature refers to a temperature condition where the sample temperature has affected the output state of the active component, causing a significant decrease in its output efficiency, but without causing sample denaturation or container melting. Therefore, obtaining real-time temperature information T... i Then, the first condition is T. i ≤T k1 If true, it indicates that the sample temperature is at or below the safe temperature, with the first target power P. k1 The output of the control unit can be used; if T i >T k1 Then the second condition is further executed, namely T. i ≤T k2If this condition is met, it means that the sample temperature is at the warning temperature but has not exceeded the danger temperature. It is necessary to reduce the output energy of the active component. See the description below for detailed steps.

[0038] S130, when the sample is in a normal state, the output of the first target power control component is used; When the sample temperature is known in real time, the sample is in a normal state. In this embodiment, this specifically means that the sample will not undergo denaturation at this temperature, and is at a safe temperature that will not affect the output state of the active component or has a minimal impact on the output of the active component, with the first target power P. k1 Control the active components to maintain their normal output state.

[0039] S140, when the sample is in a first abnormal state, the output of the active component is controlled with a second target power that is less than the first target power.

[0040] In the first abnormal state, the sample temperature is relatively high. Samples at the warning temperature have already affected the effective output of the active component. Simultaneously, the calculated energy value Q of the active component at the target power is also affected. i =P i *△t i =q i +q e +q n , where q i q represents the real-time energy value. e q represents the heat generated when the component outputs heat. n For the remaining losses, which typically account for a small percentage and fluctuate relatively little, the real-time energy value q... i The heat value q dissipated as loss under the condition of decrease e The increasing proportion may cause the sample temperature to rise further until it exceeds the danger temperature, leading to an abnormal event. This would reduce the target power output of the control unit to a level lower than the first target power P. k1 Small second target power P k2 This allows for the calculation of the energy value Q. i The temperature is reduced to further prevent the temperature from rising further due to excessive heat generated during the output of the active components. This, combined with the heat dissipation effect of the sample container, keeps the heat value q... e The heat dissipation q below that of the sample container l This causes the sample temperature to drop.

[0041] Furthermore, when the sample temperature reaches a dangerous level, it may cause sample denaturation or safety accidents. The target power adjustment process also includes: S150, determine whether the sample is in the second abnormal state based on the temperature information at the current moment; S160, if the sample is in the second abnormal state, stop the output of the active component.

[0042] Based on the description in the foregoing embodiments, the condition for determining the second abnormal state is whether the current real-time temperature information is greater than the second temperature threshold, i.e., T. i >T k2 Upon triggering this condition, the sample within the sample container may denature or even cause a safety incident. Based on this, step S160 stops the output of the active component, causing Q to... i =0, causing the sample temperature to decrease.

[0043] Furthermore, since the current temperature of the sample can sequentially jump between the three states mentioned above—the normal state, the first abnormal state, and the second abnormal state—for example, at T... k1 <T i ≤T k2 Within the range, the target power of the active component is reduced to the second target power P. k2 Then, if the real-time temperature T of the sample i Falling back to T i ≤T k1 If the range is specified, the target power is readjusted to the first target power P. k1 The corresponding adjustment voltage amplitude U i Extending this further, due to T i >T k2 If the sample temperature is within a dangerous range, the active component should be stopped and kept operating until the sample temperature returns to normal before restarting to ensure safety and prevent sample denaturation.

[0044] In this embodiment, the target power of the control component can be adjusted in real time based on temperature information, so that the output power of the control component is kept as stable as possible at or below the safe temperature. This not only protects the sample, but also significantly reduces the energy loss of the sample processing device when lysing the sample, thereby maximizing the effective output of the control component.

[0045] It should be noted that the first temperature threshold T k1 Second temperature threshold T k2 First target power P k1 Second target power P k2 The parameters can be set based on information such as the sample's hazardous temperature, sample type, sample volume, and type of active component. This invention does not impose specific limitations on these values, but all of them are within the scope of this invention.

[0046] Based on the premise of adjusting the target power using temperature information, the calculated energy value can also be adjusted accordingly at a certain moment based on the temperature information at that moment, specifically including: S221, When the sample is in a normal state, the temperature correction value does not affect the real-time energy value obtained based on the calculated energy value; Because the current temperature has a relatively small impact on the active components under normal conditions, this effect can be ignored without additional correction to the calculated energy value, thus obtaining the real-time energy value at that moment. A temperature correction factor f(T) is then introduced. i For example, in T i ≤T k1 Under the condition of f(T) i If )=1, then the real-time energy value obtained based on the calculated energy value is q. i =Q i Additionally, due to the calculation of the energy value Q... i In addition to the real-time energy value q i and calorie value q e In addition, there will be other losses q n If factors other than temperature are taken into account, additional correction factors g(T) can be introduced for the remaining losses. i ), then q i =Q i *f(T i )*g(T i When f(T) i When )=1, the real-time energy value of the active component can be obtained from the calculated energy value as q. i =Q i *g(T i (This is also within the scope of the present invention.)

[0047] S222, When the sample is in the first abnormal state, the calculated energy value is corrected according to the temperature correction value that changes with the current temperature of the sample to obtain the real-time energy value; When the sample is in the first abnormal state, the calculated energy value needs to be corrected based on temperature information. The correction coefficient can be set to be consistent. In this embodiment, the correction coefficient is set to vary with temperature, or the real-time energy value Q can be set based on theoretical functions and empirical functions, etc. i To be related to the current temperature T i Power P i The related function value is Q. i =F(T i ,P i In this embodiment, the temperature correction factor is variable, and the calculated energy value at that moment is corrected according to different temperatures.

[0048] Continue by introducing a temperature correction factor f(T) i For example, in T k1 <T i ≤T k2 In the case of temperature correction factor f(T)i ) That is, the temperature correction value is set as a linear function that varies with the real-time temperature information T i , specifically b = 1 - k1 * (T i - T k1 ) where b is the temperature correction value, i.e., the temperature correction factor f(T i ), k1 is the temperature correction coefficient, k1 > 0, and because under the first abnormal state, T i > T k1 , thus b < 1. It should be noted that b is always greater than 0 and the numerical range is set as 0 < b < 1. Under the empirical curve of the temperature information of the output energy of the acting component, between the safe temperature and the dangerous temperature, it can be recognized that there is a linear relationship between the real-time energy value q i and the calculated energy value Q i , that is, q i = m * Q i + n, thus obtaining b = 1 - k1 * (T i - T k1 ). In this embodiment, for every unit temperature value exceeded in the difference between the current temperature and the safe temperature, the temperature correction factor b also linearly decreases correspondingly. After multiplying with Q i , a real-time energy value q close to the actual output energy value is obtained i .

[0049] S223, when the sample is in the second abnormal state, the real-time energy value is zero.

[0050] Since in step S160, the acting component stops working when the sample is in the second abnormal state, at this time Q i is 0, then the corresponding real-time energy value at this moment should also be 0.

[0051] Accordingly, the real-time energy value corrected according to the temperature information at each moment during the cracking process can be obtained, so as to obtain an accurate cumulative energy value, taking the sample temperature state into account in the cracking process, making the cracking reaction more complete. Of course, in addition to the temperature correction implementation method mentioned in the above embodiment, there can also be other implementation methods for correcting the calculated energy value based on the temperature information, all within the scope of implementation of this method.

[0052] In another embodiment, in addition to considering the influence of the current temperature on the output of the acting component, the temperature rise change will also affect the output process of the acting component. For example, even if the acting component is at or below the safe temperature, but the temperature of the acting component rises rapidly during the output process, it means that the heat value q e of the differentiated calculated energy value Q iA large proportion of the energy output will result in a relatively small actual output energy value of the active component. Therefore, it is necessary to incorporate the temperature rise obtained from the temperature information into the correction of the real-time energy value.

[0053] Specifically, in S220, the calculated energy value is corrected based on the temperature information at that moment, including: S201, real-time acquisition of the current temperature of the sample at the current moment; S202, obtain the temperature rise value of the sample at the current moment based on the current temperature of the sample at the current moment and the temperature of the sample at the previous moment; During the real-time monitoring of the sample's temperature information, the previous real-time temperature information was T. i-1 Its temperature rise value is the sample temperature T at the previous moment. i-1 With the current sample temperature T i The difference between them, i.e., △T i =T i -T i-1 .

[0054] S203 calculates the energy value by correcting it based on the temperature rise, in order to obtain the real-time energy value.

[0055] Generally speaking, a higher temperature rise value indicates a lower proportion of the effective output energy of the active component at the current moment, that is, a lower real-time energy value. Therefore, the calculated energy value is corrected by the temperature rise value so that the real-time energy value is closer to the actual output energy value of the active component.

[0056] For the current moment, the effect of temperature rise on the active component can be converted into the effect of the actual working time at that moment. That is, it is assumed that the active component is only used to dissipate heat to raise the sample temperature or as the actual output of the pyrolysis reaction at a certain point in time during the entire duration of the current moment. Assume that at that moment, Δt i The temperature rise working time occupied by the temperature rise is Δt. l The effective working time occupied by the actual output is △t j Ignoring other energy losses, Δt i =△t l +△t j Then, the effective working time △t at this time j The effective proportion of the overall duration at that moment is D. i =△t j / △t i Thus, the effective working hours correction value D is obtained. i Of course, the effective working time correction value can also be estimated and confirmed using other methods, which are also within the scope of this invention.

[0057] The correction process includes the following steps: S204, when the temperature rise value does not exceed the temperature rise threshold, the calculated energy value is corrected according to the effective working time correction value that changes with the temperature rise value to obtain the real-time energy value; The set temperature rise threshold is △T. p Then the temperature rise value △T i ≤△T p Within the given range, the corresponding moments of the pyrolysis process need to be considered. For example, when the active component begins the pyrolysis process, it does not immediately output effective pyrolysis energy. Therefore, the effective working time will account for a relatively low proportion in the initial moments, while the proportion of the effective working time will gradually increase in subsequent moments until the entire time period. Thus, an effective working time correction value D is introduced. i The effective working time correction value is estimated to gradually increase after the initial time using a linear method until it reaches the effective working time correction value D. i =1, meaning the entire time period is the effective working time.

[0058] In one embodiment, the effective operating time correction value that varies with temperature rise is obtained by the following formula. D i =D i-1 +k2 In the formula, D i D is the corrected value for the effective working time at the current moment. i-1 Here, k1 is the effective working time correction value at the previous moment, k2 is the temperature rise correction coefficient, and D1 is the effective working time correction value at the start of the cracking process. Furthermore, D1 < 1, D... i ≤1.

[0059] It should be noted that the initial moment of the pyrolysis process in this embodiment refers not only to the starting moment of the entire pyrolysis process, but also to the starting moment when the pyrolysis process continues after the active component stops working under the second abnormal state.

[0060] Combining the aforementioned embodiments, the real-time energy value q i Based on the calculated energy value Q i And the effective working hours correction value D i The product of q is obtained, i.e. i =Q i *D i *△t i If a temperature correction factor f(T) is further introduced... i In this embodiment, the real-time energy value q i =f(T i )*Q i *D i *△t i .

[0061] S205, when the temperature rise value is greater than the temperature rise threshold, the effective working time correction value at that moment is consistent with the effective working time correction value at the beginning of the cracking process.

[0062] In △T i >△T p Within the specified interval, the effective operating time at that moment is estimated based on experimental experience and is consistent with the effective operating time at the start of the pyrolysis process. Therefore, the effective operating time correction value D for excessive temperature rise is calculated. x =D1, at this time the real-time energy value q i =f(T i )*Q i *D1*△t i .

[0063] Based on the description of the foregoing embodiments, the sample lysis method of the present invention corrects the calculated energy value according to the temperature information. This can be a selective correction strategy based on the current temperature and temperature rise, or an overall correction strategy that considers both temperature and temperature rise. The correction strategy can be reasonably set in combination with the actual output of the active component. In the sample processing device that lyses the sample by ultrasonic means, the overall correction strategy is selected to obtain an accurate cumulative energy value.

[0064] Based on the preceding description of sample splitting methods, please refer to... Figure 4 One embodiment of this method may specifically include the following steps: S1, set the effective working time correction value D=D1, the target power P=P1, the voltage amplitude U=U1, and the cumulative energy value Q=0, where D1 can be set to 10% in this embodiment; S2, obtain the sample temperature T, sample temperature rise ΔT and operating current I, and obtain the current power P(i)=U*I of the active component based on U and I; S3, determine whether the sample temperature T does not exceed the first temperature threshold T. k1 If yes, proceed to S31; otherwise, proceed to S4. S31, set the target power P of the control component to P1, and proceed to S32; S32, set the temperature correction value b to 1, then proceed to S6; S4, determine whether the sample temperature does not exceed the second temperature threshold T. k2 If yes, proceed to S41; otherwise, proceed to S5. S41, set the target power P of the control unit to P2, and proceed to S42; S42, set the temperature correction value b = 1 - k1 * (TT) k1 ), enter S6; S5 sets the target power P of the control unit to 0 and returns to S2; S6, determine whether the sample temperature rise ΔT does not exceed the temperature rise threshold ΔT. p If yes, proceed to S7; otherwise, proceed to S8. S7, determine whether the effective working time correction value D is 1. If yes, proceed to S9; otherwise, proceed to S71. S71, set the effective working time D=D+k2, then proceed to S72; S72, determine whether the effective working time D is greater than 1. If yes, set D=1. If not, proceed to S9. S8, set the effective working time D=D1, then proceed to S9; S9, calculate the cumulative energy value Q=Q+P(i)*D*b, then proceed to S10; S10, Determine whether the cumulative energy value Q has reached the target energy value Q. k If not, return to S2; if yes, end.

[0065] To implement the sample lysis method for a sample processing apparatus according to the first aspect embodiment, the present invention also proposes a sample lysis control system in a second aspect, see below. Figure 5 ,include: Execution unit 11 is used to control the active components of the sample lysis device to perform the lysis process on the sample; Timing unit 12 is used to record time; Acquisition unit 13 is used to acquire temperature information of the sample and monitoring information of the active component; The calculation unit 14 is used to calculate the calculated energy value of the active component during the pyrolysis process based on the monitoring information acquired by the acquisition unit 13. Correction unit 15 is used to correct the calculated energy value to a real-time energy value based on the temperature information acquired by acquisition unit 13; The processing unit 16 is used to control the execution unit 11 to perform the lysis process on the sample by the sample lysis device, control the calculation unit 14 to calculate the cumulative energy value of the active component during the lysis process based on the real-time energy value corrected by the correction unit 15, and control the execution unit 11 to stop the lysis process of the active component when the cumulative energy value reaches the target energy value.

[0066] In a third aspect, embodiments of the present invention also provide a computer-readable storage medium comprising a stored program, wherein, when the program is executed, the computer-readable storage medium controls the execution of the sample fragmentation method of the first aspect of the present invention within a device.

[0067] This invention also provides a computer device, which includes a processor, a memory, and a computer program stored in the memory and executable on the processor. When executed by the processor, the computer program implements the sample fragmentation method described in the embodiments; to avoid repetition, these details are not elaborated here. Alternatively, when executed by the processor, the computer program implements the functions of each model / unit of the control device in the embodiments; to avoid repetition, these details are not elaborated here.

[0068] Computer devices include, but are not limited to, processors and memory. Those skilled in the art will understand that the above are merely examples of computer devices and do not constitute a limitation on computer devices. A computer device may include more or fewer components than illustrated, or a combination of certain components, or different components. For example, a computer device may also include input / output devices, network access devices, buses, etc.

[0069] The processor referred to can be a Central Processing Unit (CPU), or other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. A general-purpose processor can be a microprocessor or any conventional processor.

[0070] Memory can be an internal storage unit of a computer device, such as a hard drive or RAM. Memory can also be an external storage device of a computer device, such as a plug-in hard drive, Smart Media Card (SMC), Secure Digital (SD) card, or Flash Card. Furthermore, memory can include both internal and external storage units. Memory is used to store computer programs and other programs and data required by the computer device. Memory can also be used to temporarily store data that has been output or will be output.

[0071] In the above embodiments, implementation can be achieved entirely or partially through software, hardware, firmware, or any combination thereof. When implemented using software, it can be implemented entirely or partially in the form of a computer program product. The computer program product includes one or more computer instructions. When the computer instructions are loaded and executed on a computer, all or part of the processes or functions described in the embodiments of this application are generated. The computer can be a general-purpose computer, a special-purpose computer, a computer network, or other programmable device. The computer instructions can be stored in a computer-readable storage medium or transmitted from one computer-readable storage medium to another. For example, the computer instructions can be transmitted from one website, computer, server, or data center to another website, computer, server, or data center via wired (e.g., coaxial cable, fiber optic, Digital Subscriber Line (DSL)) or wireless (e.g., infrared, wireless, microwave, etc.) means. The computer-readable storage medium can be any available medium accessible to a computer or a data storage device such as a server or data center that integrates one or more available media. The available media may be magnetic media (e.g., floppy disks, hard disks, magnetic tapes), optical media (e.g., high-density digital video discs (DVDs)), or semiconductor media (e.g., solid-state drives (SSDs)).

[0072] While specific embodiments of the invention have been described in detail by way of examples, those skilled in the art should understand that the examples are for illustrative purposes only and not intended to limit the scope of the invention. Those skilled in the art should understand that modifications can be made to the above embodiments without departing from the scope and spirit of the invention. The scope of the invention is defined by the appended claims.

[0073] Finally, it should be noted that the above description is merely the preferred embodiment of the present invention and is not intended to limit the present invention in any way. Any person skilled in the art can make many possible variations and simple substitutions to the technical solutions of the present invention using the disclosed methods and techniques without departing from the scope of the present invention; all of these variations fall within the protection scope of the present invention.

Claims

1. A sample lysis method applied to a sample processing device, characterized in that, include: The target power control component performs the lysis process on the sample; Record the real-time energy value of the active component during the pyrolysis process, and calculate the cumulative energy value of the active component during the pyrolysis process based on the real-time energy value; When the accumulated energy value reaches the target energy value, the decomposition process of the active component is stopped; When the real-time energy value is recorded at various times, the method further includes: The calculated energy value at that moment is obtained based on the real-time power output by the active component at that moment; The temperature information of the sample at that time is obtained, and the calculated energy value is corrected based on the temperature information at that time to obtain the real-time energy value at that time.

2. The method according to claim 1, characterized in that, When the target power control component performs the pyrolysis process on the sample, it adjusts the target power according to the temperature information of the sample at the current moment; The target power adjustment process includes the following steps: When the active component begins to perform the lysis process on the sample, the output of the active component is controlled with a first target power; Based on the current temperature information, determine whether the sample is in a normal state or a first abnormal state; When the sample is in a normal state, the output of the active component is controlled by the first target power; When the sample is in a first abnormal state, the output of the active component is controlled with a second target power that is less than the first target power.

3. The method according to claim 2, characterized in that, The step of determining whether the sample is in a normal state or a first abnormal state based on the current temperature information includes: The current temperature of the sample is obtained in real time at the current moment; If the current temperature does not exceed the first temperature threshold, the sample is determined to be in a normal state. If the current temperature is greater than the first temperature threshold and less than the second temperature threshold, the sample is determined to be in a first abnormal state. The target power adjustment process also includes: Determine whether the sample is in a second abnormal state based on the current temperature information; If the sample is in a second abnormal state, the output of the active component is stopped.

4. The method according to claim 3, characterized in that, The correction of the calculated energy value based on the temperature information at that moment includes: When the sample is in a normal state, the temperature correction value does not affect the real-time energy value obtained based on the calculated energy value; When the sample is in a first abnormal state, the calculated energy value is corrected according to the temperature correction value that changes with the current temperature of the sample to obtain the real-time energy value; When the sample is in the second abnormal state, the real-time energy value is zero.

5. The method according to claim 4, characterized in that, When the sample is in the first abnormal state, the temperature correction value, which varies with the current temperature of the sample, is obtained by the following formula. b=1-k1*(T i -T k1 ) Where b is the temperature correction value, k1 is the temperature correction coefficient, and T i T represents the current temperature of the sample. k1 Let b be the first temperature threshold, and b < 1; When the sample is in the first abnormal state, the real-time energy value is obtained by multiplying the calculated energy value and the temperature correction value.

6. The method according to any one of claims 1 to 5, characterized in that, The correction of the calculated energy value based on the temperature information at that moment includes: The current temperature of the sample is obtained in real time at the current moment; The temperature rise of the sample at the current moment is obtained based on the current temperature of the sample at the current moment and the temperature of the sample at the previous moment; The calculated energy value is corrected based on the temperature rise value to obtain the real-time energy value.

7. The method according to claim 6, characterized in that, The calculated energy value is corrected based on the temperature rise value, including: When the temperature rise value does not exceed the temperature rise threshold, the calculated energy value is corrected according to the effective working time correction value that changes with the temperature rise value to obtain the real-time energy value; When the temperature rise value is greater than the temperature rise threshold, the effective working time correction value at that moment is consistent with the effective working time correction value at the start of the pyrolysis process.

8. The method according to claim 7, characterized in that, When the temperature rise value does not exceed the temperature rise threshold, the effective working time correction value that varies with the temperature rise value is obtained by the following formula. D i =D i-1 +k2 Among them, D i D is the corrected value for the effective working time at the current moment. i-1 Where k1 is the effective working time correction value at the previous moment, k2 is the temperature rise correction coefficient, and D1 is the effective working time correction value at the start of the cracking process, and D1 < 1, D i ≤1; The real-time energy value is obtained by multiplying the calculated energy value and the effective working time correction value.

9. A sample lysis control system, applied to a sample processing device, characterized in that, include: An execution unit is used to control the active components of the sample lysis device to perform the lysis process on the sample; A timing unit used to record time; The acquisition unit is used to acquire the temperature information of the sample and the monitoring information of the functional component; A calculation unit is used to calculate the calculated energy value of the active component during the pyrolysis process based on the monitoring information acquired by the acquisition unit. The correction unit is used to correct the calculated energy value to a real-time energy value based on the temperature information acquired by the acquisition unit. The processing unit is used to control the execution unit to cause the sample lysis device to perform a lysis process on the sample, control the calculation unit to calculate the cumulative energy value of the active component during the lysis process based on the real-time energy value corrected by the correction unit, and control the execution unit to stop the lysis process of the active component when the cumulative energy value reaches the target energy value.

10. A computer-readable storage medium, characterized in that, The computer-readable storage medium stores a computer program that, when executed by a processor, performs the sample fragmentation method as described in any one of claims 1 to 8.

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