Temperature-sensitive change gel spray composition and preparation method thereof

Abstract

The invention discloses a temperature-sensitive change gel spray composition and a preparation method thereof, and belongs to the field of gel spray. The temperature-sensitive change gel spray composition provided by the invention is prepared by the following steps: firstly, dissolving soybean lecithin and cholesterol in a solvent, adding a fructo-oligosaccharide aqueous solution to prepare a temperature-sensitive liposome, and meanwhile, mixing low-acyl gellan gum, high-acyl gellan gum, xanthan gum and deionized water I to form a solution; sequentially adding a humectant, trehalose, panthenol and menthyl lactate into the solution, and mixing to form a main water phase; adding oxidized hyaluronic acid into the main water phase, then adding a chitosan-lactic acid mixed solution to adjust the pH value, and continuing to add temperature-sensitive lipidosome and rose essential oil nano-emulsion for mixing; and finally, adding a calcium chloride solution and deionized water II, and filtering. The gel spray composition provided by the invention has excellent moisturizing capacity and film-forming property, can regulate micro-ecological balance, and realizes refreshing spray experience and long-acting moisturizing.

Description

Technical Field

[0001] This invention belongs to the field of gel spraying, specifically relating to a temperature-sensitive gel spray composition and its preparation method. Background Technology

[0002] In recent years, with the popularization of fast-paced lifestyles and the rise of the "light skincare" concept, moisturizing gel sprays have become popular products for various scenarios, including daily skincare, post-sun soothing, makeup setting, and cooling after exercise. Consumers generally expect them to have an immediate cooling sensation, a refreshing and non-sticky feel, convenient spray application, and long-lasting moisturizing effects.

[0003] Existing products are mostly based on water, glycerin, hyaluronic acid, and a small amount of film-forming polymers (such as carbomer or cellulose derivatives), and are atomized through aerosol cans or pump nozzles to provide rapid hydration and a short-lived cooling sensation. However, these traditional sprays generally suffer from several technical defects: First, the gel system used is highly viscous at room temperature, resulting in uneven spraying, large droplets, and difficulty in forming a uniform coverage; second, moisturizing ingredients are mostly released only once and cannot lock in moisture on the skin surface for a long time, with water and active ingredients evaporating rapidly in a short time, which can actually exacerbate dryness; third, cooling ingredients (such as menthol) are highly irritating and released too quickly, easily causing stinging or erythema, and their duration is short; in addition, the film-forming structure of existing sprays is a static cross-linked network, which cannot self-repair once damaged by friction, sweating, or cleaning, and its protective function is immediately lost; finally, the formulations generally neglect the skin's microecological balance and lack selective support for beneficial bacteria, which may disrupt the skin barrier homeostasis and weaken the skin's natural defense capabilities with long-term use. Therefore, it is essential to develop a novel thermosensitive gel spray composition. Summary of the Invention

[0004] In view of the shortcomings of the prior art, the purpose of this invention is to provide a temperature-sensitive gel spray composition and its preparation method.

[0005] The first aspect of this invention is to provide a method for preparing a temperature-sensitive gel spray composition, comprising the following steps: S1: Soybean lecithin and cholesterol were dissolved in a solvent, and an aqueous solution of oligofructose was added to prepare thermosensitive liposomes; S2: Mix low-acyl gellan gum, high-acyl gellan gum, xanthan gum and deionized water to form a solution; S3: Add humectant, trehalose, panthenol and menthyl lactate to the solution in sequence to form the main aqueous phase; S4: Add oxidized hyaluronic acid to the main aqueous phase, followed by adding chitosan-lactic acid mixture to adjust the pH value; S5: Add thermosensitive liposomes and rose essential oil nanoemulsion to the solution system of S4 and mix them; S6: Add calcium chloride solution to the solution system of S5, add deionized water, and filter to obtain the temperature-sensitive gel spray composition.

[0006] It should be noted that this invention creatively combines low-acyl gellan gum LA and high-acyl gellan gum HA, co-dissolves them in an aqueous phase in a specific ratio, and then reacts them with divalent cations (such as Ca). 2+ A gradient-responsive composite gel system was constructed in the presence of [a specific substance / component]. Under heating (≈70℃) and stirring conditions, the HA and LA molecular chains fully detangled, forming a homogeneous and transparent solution. As the temperature decreased, the two types of molecules underwent conformational transformations and ionic cross-linking sequentially at different temperature ranges based on their differences in acyl group content. Due to the steric hindrance effect of the side chain acyl groups, the HA molecular chains were randomly coiled, requiring cooling at a higher temperature (approximately 65-75℃) to begin forming a soft, elastic, and opaque weak gel network through hydrogen bonding and hydrophobic interactions. LA, due to the increased chain rigidity after deacylation, could form a gel network at a lower temperature using Ca[a specific substance / component]. 2+ Under bridging, a rigid gel network is formed by stacking double helical structures, resulting in a hard, brittle, transparent, and high-strength structure. When the two coexist, in the intermediate temperature range, the HA network provides elasticity and water retention, while the LA network provides structural strength, forming a physical network.

[0007] In some embodiments, the mass ratio of soybean lecithin to cholesterol is 6-8:3; the mass-volume concentration of the fructooligosaccharide aqueous solution is 5-10%, and the dry weight of the fructooligosaccharide is 15-25% of the sum of the masses of soybean lecithin and cholesterol.

[0008] In some embodiments, the solvent is anhydrous ethanol; the ratio of the solvent to the sum of the masses of soybean lecithin and cholesterol is 75-100 mL: 2-3 g.

[0009] In some embodiments, the low-acyl gellan gum has an acetyl content ≤0.5% and a glyceryl content ≤1.0%; the high-acyl gellan gum has an acetyl content of 2.5-4.0% and a glyceryl content of 6.0-8.0%; and the amount of deionized water used is 155-165 times the sum of the masses of the low-acyl gellan gum, the high-acyl gellan gum, and xanthan gum.

[0010] As is generally known, the basic repeating unit of gellan gum is a tetrasaccharide (2 glucose + 1 glucuronic acid + 1 rhamnose). High-acyl gellan gum (gellan-HA) contains an average of 1 glyceryl acyl group and 0.4-0.6 acetyl groups per tetrasaccharide unit. Converted to mass fraction (total acyl groups as a percentage of dry weight): acetyl content ≈ 2.5-4.0% (w / w), glyceryl acyl content ≈ 6.0-8.0% (w / w). Low-acyl gellan gum (gellan-LA), after alkali treatment, has a deacylation degree ≥90%, that is, it retains ≤10% of the original acyl groups. The residual amount per tetrasaccharide unit is: ≤0.1 glyceryl acyl group and ≤0.05 acetyl group. The corresponding mass fraction is: acetyl content ≤0.3-0.5% (w / w), glyceryl acyl content ≤0.6-1.0% (w / w).

[0011] In some embodiments, the chitosan-lactic acid mixture is prepared by mixing chitosan and lactic acid aqueous solution in a mass ratio of 0.01-0.03 g: 1 mL; the mass volume concentration of the lactic acid aqueous solution is 0.4-0.6%; and the pH value is adjusted to 5.2-5.6.

[0012] In some embodiments, in S1, the preparation temperature is 4-6°C; in S2, the mixing is performed by shearing at 5800-6200 rpm for 8-12 minutes at 68-75°C.

[0013] In some embodiments, in S3, the mixing temperature is 45-55°C; in S5, the mixing temperature is 35-45°C; and in S6, the filter pore size is 0.4-0.5 μm.

[0014] A second aspect of the present invention is to provide a sensitive change gel spray composition.

[0015] In some embodiments, the amounts of each component in the thermosensitive gel spray composition, by weight, are as follows: 0.4-0.7 parts thermosensitive liposomes, 0.1-0.2 parts low-acyl gellan gum, 0.05-0.15 parts high-acyl gellan gum, 0.03-0.07 parts xanthan gum, 8-12 parts humectant, 1-3 parts trehalose, 0.5-1.5 parts panthenol, 0.2-0.5 parts menthyl lactate, 0.1-0.3 parts oxidized hyaluronic acid, 0.2-0.3 parts chitosan-lactic acid mixture, 0.8-1.2 parts rose essential oil nanoemulsion, 0.1-0.2 parts calcium chloride solution, and 10-20 parts deionized water.

[0016] In this invention, the preparation of rose essential oil nanoemulsion is not limited. It can be obtained by the following method: Rose essential oil, soybean lecithin, polyglycerol-10 laurate and 1,2-pentanediol are mixed in a mass ratio of 10:5:2:3, stirred in a water bath at 40°C until a clear oil phase is obtained, and an appropriate amount of deionized water preheated to 40°C is added. The rose essential oil nanoemulsion is obtained by high-speed shearing.

[0017] In some embodiments, the humectant is selected from at least one of glycerin, butylene glycol, and 1,2-pentanediol.

[0018] Compared with the prior art, the present invention has the following beneficial effects: 1. The thermosensitive gel spray composition provided by the present invention, by compounding high-acyl and low-acyl gellan gum, constructs a composite system with thermal response characteristics under the regulation of divalent cations. It can maintain a low viscosity fluid state at room temperature, ensuring that the spray is fine, uniform and non-clogging. Once in contact with the skin (≥33℃), it can form a transparent, strong and breathable three-dimensional gel film, achieving immediate water locking and sustained release of active ingredients, and achieving a synergistic effect of refreshing spray experience and long-lasting moisturizing.

[0019] 2. This invention introduces aldehyde-containing polysaccharides (oxidized hyaluronic acid) and primary amino-containing natural polycations (chitosan) into the formulation. The two undergo a Schiff base reaction in the aqueous phase under weakly acidic conditions, which can proceed slowly at room temperature. After being sprayed onto the skin surface, water evaporation promotes the molecular chains to approach each other, accelerating the cross-linking of imine bonds to form a three-dimensional network gel film. When the film develops microcracks due to friction, sweat washing, or mechanical stretching, the -CHO and -NH2 groups at the fracture sites re-contact and spontaneously recombine under the water medium, maintaining the integrity of the barrier. At the same time, this invention also integrates thermosensitive liposomes for sustained-release of fructooligosaccharides. FOS is pre-encapsulated in thermosensitive liposomes. The liposomes are stable at room temperature, but the phospholipid bilayer fluidity increases and the pores expand at skin temperature, achieving a gentle and sustained release of FOS. The released FOS, as a selective prebiotic, is preferentially metabolized and utilized by epidermal symbiotic bacteria, selectively nourishing beneficial bacteria and inhibiting harmful bacteria, actively regulating the skin microecological balance, and significantly improving moisturizing durability, skin comfort, and long-term skin health.

[0020] 3. This invention also incorporates menthyl lactate as a sustained-release cooling agent into the thermosensitive gel spray composition, which slowly releases a mild cooling sensation, avoiding the irritation and instantaneous volatilization defects of traditional menthol; and adds moisturizers, trehalose and panthenol to achieve moisture absorption, water retention, long-lasting moisturization and barrier repair, reducing transdermal water loss; in addition, this invention also incorporates rose essential oil nanoemulsion, which is rich in natural aromatic molecules such as citronellol, geraniol, and phenethyl alcohol, and has the ability to scavenge free radicals and inhibit inflammatory factors, and can promote the penetration of active ingredients. Detailed Implementation

[0021] The present invention will now be described in further detail with reference to specific embodiments.

[0022] Example 1 A method for preparing a temperature-sensitive gel spray composition includes the following steps: S1: Soybean lecithin and cholesterol in a mass ratio of 7:3 were dissolved in anhydrous ethanol, and an 8% (w / v) aqueous solution of oligofructose was added to prepare thermosensitive liposomes at 5°C; wherein the dry weight of oligofructose was 20% of the sum of the masses of soybean lecithin and cholesterol, and the ratio of anhydrous ethanol to the sum of the masses of soybean lecithin and cholesterol was 85 mL: 2.5 g. S2: 0.15 parts by weight of low-acyl gellan gum, 0.1 parts by weight of high-acyl gellan gum, 0.05 parts by weight of xanthan gum, and deionized water are sheared at 70°C and 6000 rpm for 10 min to form a solution; wherein, the low-acyl gellan gum has an acetyl content ≤0.5% and a glyceryl content ≤1.0%; the high-acyl gellan gum has an acetyl content of 2.5-4.0% and a glyceryl content of 6.0-8.0%; the amount of deionized water is 160 times the sum of the masses of low-acyl gellan gum, high-acyl gellan gum, and xanthan gum; S3: Add 10 parts by weight of glycerol, 2 parts by weight of trehalose, 1 part by weight of panthenol and 0.35 parts by weight of menthyl lactate to the solution in sequence and mix at 50°C to form the main aqueous phase; S4: Add 0.2 parts by weight of oxidized hyaluronic acid to the main aqueous phase, followed by 0.25 parts by weight of chitosan-lactic acid mixture to adjust the pH to 5.2-5.6; wherein, the chitosan-lactic acid mixture is composed of chitosan and lactic acid aqueous solution in a mass ratio of 0.02g:1mL, and the mass-volume concentration of the lactic acid aqueous solution is 0.5%; S5: Add 0.5 parts by weight of thermosensitive liposomes and 1 part by weight of rose essential oil nanoemulsion to the solution system of S4 and mix at 40°C; S6: Add 0.15 parts by weight of calcium chloride solution to the solution system of S5, add 15 parts by weight of deionized water, and filter through a 0.45 μm filter membrane to obtain the temperature-sensitive gel spray composition.

[0023] The rose essential oil nanoemulsion of the present invention is prepared by the following steps: Rose essential oil, soybean lecithin, polyglycerol-10 laurate and 1,2-pentanediol are mixed in a mass ratio of 10:5:2:3, stirred in a water bath at 40°C until a clear oil phase is obtained, and an appropriate amount of deionized water preheated to 40°C is added. The rose essential oil nanoemulsion is obtained by high-speed shearing.

[0024] Example 2 A method for preparing a temperature-sensitive gel spray composition includes the following steps: S1: Soybean lecithin and cholesterol in a mass ratio of 8:3 were dissolved in anhydrous ethanol, and a 10% (w / v) aqueous solution of oligofructose was added to prepare thermosensitive liposomes at 6°C; wherein, the dry weight of oligofructose was 25% of the sum of the masses of soybean lecithin and cholesterol, and the ratio of anhydrous ethanol to the sum of the masses of soybean lecithin and cholesterol was 100 mL: 3 g. S2: 0.2 parts by weight of low-acyl gellan gum, 0.15 parts by weight of high-acyl gellan gum, 0.07 parts by weight of xanthan gum, and deionized water were sheared at 75°C and 6200 rpm for 8 minutes to form a solution; wherein, the acetyl content of low-acyl gellan gum is ≤0.5% and the glyceryl content is ≤1.0%; the acetyl content of high-acyl gellan gum is 2.5-4.0% and the glyceryl content is 6.0-8.0%; the mass of deionized water is 165 times the sum of the masses of low-acyl gellan gum, high-acyl gellan gum, and xanthan gum; S3: Add 12 parts by weight of butanediol, 3 parts by weight of trehalose, 1.5 parts by weight of panthenol and 0.5 parts by weight of menthyl lactate to the solution in sequence and mix at 55°C to form the main aqueous phase; S4: Add 0.3 parts by weight of oxidized hyaluronic acid to the main aqueous phase, followed by 0.3 parts by weight of chitosan-lactic acid mixture to adjust the pH to 5.2-5.6; wherein, the chitosan-lactic acid mixture is composed of chitosan and lactic acid aqueous solution with a mass ratio of 0.03g:1mL, and the mass-volume concentration of the lactic acid aqueous solution is 0.6%; S5: Add 0.7 parts by weight of thermosensitive liposomes and 1.2 parts by weight of rose essential oil nanoemulsion to the solution system of S4 and mix at 45°C; S6: Add 0.2 parts by weight of calcium chloride solution to the solution system of S5, add 20 parts by weight of deionized water, and filter through a 0.5 μm filter membrane to obtain the temperature-sensitive gel spray composition.

[0025] The preparation steps of the rose essential oil nanoemulsion are the same as in Example 1.

[0026] Example 3 A method for preparing a temperature-sensitive gel spray composition includes the following steps: S1: Soybean lecithin and cholesterol in a mass ratio of 6:3 were dissolved in anhydrous ethanol, and a 5% (w / v) aqueous solution of oligofructose was added to prepare thermosensitive liposomes at 4°C; wherein, the dry weight of oligofructose was 15% of the sum of the masses of soybean lecithin and cholesterol, and the ratio of anhydrous ethanol to the sum of the masses of soybean lecithin and cholesterol was 75 mL: 2 g. S2: 0.1 parts by weight of low-acyl gellan gum, 0.05 parts by weight of high-acyl gellan gum, 0.03 parts by weight of xanthan gum, and deionized water are sheared at 5800 rpm for 8 minutes at 68°C to form a solution; wherein, the acetyl content of low-acyl gellan gum is ≤0.5% and the glyceryl content is ≤1.0%; the acetyl content of high-acyl gellan gum is 2.5-4.0% and the glyceryl content is 6.0-8.0%; the mass of deionized water is 155 times the sum of the masses of low-acyl gellan gum, high-acyl gellan gum, and xanthan gum; S3: Add 8 parts by weight of 1,2-pentanediol, 1 part by weight of trehalose, 0.5 parts by weight of panthenol and 0.2 parts by weight of menthyl lactate to the solution in sequence and mix at 45°C to form the main aqueous phase; S4: Add 0.1 parts by weight of oxidized hyaluronic acid to the main aqueous phase, followed by 0.2 parts by weight of chitosan-lactic acid mixture to adjust the pH to 5.2-5.6; wherein, the chitosan-lactic acid mixture is composed of chitosan and lactic acid aqueous solution in a mass ratio of 0.01g:1mL, and the mass-volume concentration of the lactic acid aqueous solution is 0.4%; S5: Add 0.4 parts by weight of thermosensitive liposomes and 0.8 parts by weight of rose essential oil nanoemulsion to the solution system of S4 and mix at 35°C; S6: Add 0.1 parts by weight of calcium chloride solution to the solution system of S5, add 10 parts by weight of deionized water, and filter through a 0.4 μm filter membrane to obtain the temperature-sensitive gel spray composition.

[0027] The preparation steps of the rose essential oil nanoemulsion are the same as in Example 1.

[0028] Example 4 A method for preparing a temperature-sensitive gel spray composition includes the following steps: S1: Soybean lecithin and cholesterol in a mass ratio of 6.5:3 were dissolved in anhydrous ethanol, and a 6% (w / v) aqueous solution of oligofructose was added to prepare thermosensitive liposomes at 5°C; wherein, the dry weight of oligofructose was 18% of the sum of the masses of soybean lecithin and cholesterol, and the ratio of anhydrous ethanol to the sum of the masses of soybean lecithin and cholesterol was 80 mL: 2.5 g; S2: 0.2 parts by weight of low-acyl gellan gum, 0.05 parts by weight of high-acyl gellan gum, 0.04 parts by weight of xanthan gum, and deionized water were sheared at 5900 rpm for 9 minutes at 72°C to form a solution; wherein, the acetyl content of low-acyl gellan gum is ≤0.5% and the glyceryl content is ≤1.0%; the acetyl content of high-acyl gellan gum is 2.5-4.0% and the glyceryl content is 6.0-8.0%; the mass of deionized water is 158 times the sum of the masses of low-acyl gellan gum, high-acyl gellan gum, and xanthan gum; S3: Add 9 parts by weight of glycerol, 2 parts by weight of trehalose, 0.8 parts by weight of panthenol and 0.4 parts by weight of menthyl lactate to the solution in sequence and mix at 50°C to form the main aqueous phase; S4: Add 0.15 parts by weight of oxidized hyaluronic acid to the main aqueous phase, followed by 0.25 parts by weight of chitosan-lactic acid mixture to adjust the pH to 5.2-5.6; wherein, the chitosan-lactic acid mixture is composed of chitosan and lactic acid aqueous solution in a mass ratio of 0.02g:1mL, and the mass-volume concentration of the lactic acid aqueous solution is 0.5%; S5: Add 0.6 parts by weight of thermosensitive liposomes and 0.9 parts by weight of rose essential oil nanoemulsion to the solution system of S4 and mix at 40°C; S6: Add 0.15 parts by weight of calcium chloride solution to the solution system of S5, add 13 parts by weight of deionized water, and filter through a 0.45 μm filter membrane to obtain the temperature-sensitive gel spray composition.

[0029] The preparation steps of the rose essential oil nanoemulsion are the same as in Example 1.

[0030] Example 5 A method for preparing a temperature-sensitive gel spray composition includes the following steps: S1: Soybean lecithin and cholesterol in a mass ratio of 8:3 were dissolved in anhydrous ethanol, and a 9% (w / v) aqueous solution of oligofructose was added to prepare thermosensitive liposomes at 6°C; wherein, the dry weight of oligofructose was 22% of the sum of the masses of soybean lecithin and cholesterol, and the ratio of anhydrous ethanol to the sum of the masses of soybean lecithin and cholesterol was 95 mL: 2.5 g; S2: 0.15 parts by weight of low-acyl gellan gum, 0.1 parts by weight of high-acyl gellan gum, 0.06 parts by weight of xanthan gum, and deionized water were sheared at 75°C and 6100 rpm for 10 min to form a solution; wherein, the acetyl content of low-acyl gellan gum is ≤0.5% and the glyceryl content is ≤1.0%; the acetyl content of high-acyl gellan gum is 2.5-4.0% and the glyceryl content is 6.0-8.0%; the mass of deionized water is 163 times the sum of the masses of low-acyl gellan gum, high-acyl gellan gum, and xanthan gum; S3: Add 11 parts by weight of glycerol, 3 parts by weight of trehalose, 1.5 parts by weight of panthenol and 0.4 parts by weight of menthyl lactate to the solution in sequence and mix at 50°C to form the main aqueous phase; S4: Add 0.2 parts by weight of oxidized hyaluronic acid to the main aqueous phase, followed by 0.25 parts by weight of chitosan-lactic acid mixture to adjust the pH to 5.2-5.6; wherein, the chitosan-lactic acid mixture is composed of chitosan and lactic acid aqueous solution in a mass ratio of 0.02g:1mL, and the mass-volume concentration of the lactic acid aqueous solution is 0.5%; S5: Add 0.6 parts by weight of thermosensitive liposomes and 1.1 parts by weight of rose essential oil nanoemulsion to the solution system of S4 and mix at 40°C; S6: Add 0.15 parts by weight of calcium chloride solution to the solution system of S5, add 18 parts by weight of deionized water, and filter through a 0.5 μm filter membrane to obtain the temperature-sensitive gel spray composition.

[0031] The preparation steps of the rose essential oil nanoemulsion are the same as in Example 1.

[0032] Comparative Example 1 It is basically the same as Example 1, except that only a single gellan gum is used, that is, the high acyl gellan gum is replaced with an equal amount of low acyl gellan gum.

[0033] Comparative Example 2 It is basically the same as Example 1, except that temperature-sensitive liposomes are not added.

[0034] Comparative Example 3 It is basically the same as Example 1, except that no fructooligosaccharides are added.

[0035] Comparative Example 4 It is basically the same as Example 1, except that oxidized hyaluronic acid is not added.

[0036] The gel spray compositions prepared in Examples 1-5 and Comparative Examples 1-4 were tested, and the test results are shown in Table 1.

[0037] Temperature-sensitive film-forming property test: Rheological time scanning was used, with the temperature increasing from 25℃ to 35℃ at a rate of 2℃ / min, and then held at 35℃. The gelation time and gel strength were tested using an ARES-G2 rheometer. Moisturizing efficacy test: The subjects were 30 healthy women aged 25-45 years. The test was conducted on the inner forearm, with a single spray of 0.1 mL / cm². 2 Measure the baseline TEWL value, measure TEWT at 2 hours, and calculate the TEWL reduction rate = (baseline - time t) / baseline × 100%; Repair capability test: The scratch-recovery microscopy method was used. 0.5 mL of sample was dropped onto a glass slide and dried at 35℃ to form a film (about 5 min). A 100 μm wide scratch was made with a 27G needle and the slide was immediately placed in a 35℃, 90% RH environment. Images were taken at 0s, 30s, and 60s using an optical microscope (20×). The scratch area residual rate was analyzed using ImageJ software, and the repair rate was calculated as (initial area - area at time t) / initial area × 100%. Microecological regulation test: The samples were co-cultured in vitro. 1 mL of each sample was inoculated with an equal volume (10... 5 Two strains (CFU / mL) were cultured in Mueller-Hinton broth anaerobically at 37°C for 24 h. CFU were counted by plating, and the growth ratio of Staphylococcus epidermidis / Staphylococcus aureus was calculated. A ratio >5:1 was considered to significantly promote the microecological balance. Sensory evaluation test: A total of 30 subjects (half male and half female, including 10 with sensitive skin) were randomly assigned to a double-blind study. After cleansing their faces and balancing for 30 minutes, the sample was sprayed on them. They were scored immediately (0 min) and 5 min later. The scoring system was 5 points, with 1 point being very sticky and uncomfortable, and 5 points being extremely refreshing and comfortable.

[0038] Table 1 Test Results As can be seen from Table 1, the temperature-sensitive gel spray compositions provided in Examples 1-5 of the present invention have excellent film-forming properties, moisturizing power, and the ability to ensure barrier integrity and long-lasting adhesion. At the same time, they can regulate the microecological balance, enhance the biological protection function, and bring users a refreshing and non-greasy experience.

[0039] As can be seen from the comparative examples, Comparative Example 1 did not use a combination of high-acyl and low-acyl gellan gels. The single LA gelled partially at room temperature, lacking the elastic support of HA, resulting in a significant decrease in gel strength and insufficient water-locking capacity. Comparative Example 2 lacked thermosensitive liposomes, so FOS could not be released slowly, and beneficial bacteria could not obtain nutrients. The Staphylococcus epidermidis / Staphylococcus aureus ratio dropped sharply from 8.7:1 to 2.3:1. Comparative Example 3 removed FOS, and was similar to Comparative Example 2, confirming that FOS is the source of prebiotic activity, rather than the liposome material itself, and that fructooligosaccharides are the core functional component for regulating the skin flora. Comparative Example 4 lacked oxidized hyaluronic acid, only containing chitosan, which could not cross-link, and the repair rate dropped sharply from 92.5% to 18.3%.

[0040] The above descriptions are merely some embodiments of the present invention. For those skilled in the art, various modifications and improvements can be made without departing from the inventive concept of the present invention, and these all fall within the protection scope of the present invention.

Claims

1. A method for preparing a temperature-sensitive gel spray composition, characterized in that, Includes the following steps: S1: Soybean lecithin and cholesterol were dissolved in a solvent, and an aqueous solution of oligofructose was added to prepare thermosensitive liposomes; S2: Mix low-acyl gellan gum, high-acyl gellan gum, xanthan gum and deionized water to form a solution; S3: Add humectant, trehalose, panthenol and menthyl lactate to the solution in sequence to form the main aqueous phase; S4: Add oxidized hyaluronic acid to the main aqueous phase, and then add chitosan-lactic acid mixture to adjust the pH value; S5: Add the thermosensitive liposomes and rose essential oil nanoemulsion to the solution system of S4 and mix them; S6: Add calcium chloride solution to the solution system of S5, add deionized water, and filter to obtain the temperature-sensitive gel spray composition.

2. The method for preparing the thermosensitive gel spray composition according to claim 1, characterized in that, The mass ratio of soybean lecithin to cholesterol is 6-8:3; the mass-volume concentration of the fructooligosaccharide aqueous solution is 5-10%, and the dry weight of the fructooligosaccharide is 15-25% of the sum of the masses of soybean lecithin and cholesterol.

3. The method for preparing the thermosensitive gel spray composition according to claim 1, characterized in that, The solvent is anhydrous ethanol; the ratio of the solvent to the sum of the masses of the soybean lecithin and the cholesterol is 75-100 mL: 2-3 g.

4. The method for preparing the thermosensitive gel spray composition according to claim 1, characterized in that, The low-acyl gellan gum has an acetyl content ≤0.5% and a glyceryl content ≤1.0%; the high-acyl gellan gum has an acetyl content of 2.5-4.0% and a glyceryl content of 6.0-8.0%; the amount of deionized water is 155-165 times the sum of the masses of the low-acyl gellan gum, the high-acyl gellan gum, and the xanthan gum.

5. The method for preparing the thermosensitive gel spray composition according to claim 1, characterized in that, The chitosan-lactic acid mixture is prepared by mixing chitosan and lactic acid aqueous solution in a mass ratio of 0.01-0.03g:1mL; the mass volume concentration of the lactic acid aqueous solution is 0.4-0.6%; and the pH value is adjusted to 5.2-5.

6.

6. The method for preparing the thermosensitive gel spray composition according to claim 1, characterized in that, In step S1, the preparation temperature is 4-6℃; in step S2, the mixing is performed by shearing at 5800-6200 rpm for 8-12 minutes at 68-75℃.

7. The method for preparing the thermosensitive gel spray composition according to claim 1, characterized in that, In step S3, the mixing temperature is 45-55℃; in step S5, the mixing temperature is 35-45℃; and in step S6, the filter pore size is 0.4-0.5μm.

8. A thermosensitive gel spray composition prepared by the method of any one of claims 1-7.

9. The thermosensitive gel spray composition according to claim 8, characterized in that, The amounts of each component in the thermosensitive gel spray composition, by weight, are as follows: 0.4-0.7 parts thermosensitive liposomes, 0.1-0.2 parts low-acyl gellan gum, 0.05-0.15 parts high-acyl gellan gum, 0.03-0.07 parts xanthan gum, 8-12 parts humectant, 1-3 parts trehalose, 0.5-1.5 parts panthenol, 0.2-0.5 parts menthyl lactate, 0.1-0.3 parts oxidized hyaluronic acid, 0.2-0.3 parts chitosan-lactic acid mixture, 0.8-1.2 parts rose essential oil nanoemulsion, 0.1-0.2 parts calcium chloride solution, and 10-20 parts deionized water.

10. The thermosensitive gel spray composition according to claim 9, characterized in that, The moisturizer is selected from at least one of glycerin, butylene glycol, and 1,2-pentanediol.

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