Preparation method of phellinus igniarius concentrated extract and product

Abstract

The preparation method comprises the following steps: taking a proper amount of dried phellinus igniarius powder, further drying the phellinus igniarius powder in a dryer until the weight of the phellinus igniarius powder is constant, removing residual moisture, and sieving the dried phellinus igniarius powder; adding the pretreated phellinus igniarius powder into an extraction solvent according to the mass ratio of 1: 10, uniformly mixing, putting the mixed solution into an ultrasonic extractor, and setting the ultrasonic frequency to be 40kHz, the power to be 200W and the extraction time to be 30 minutes; filtering the mixed solution subjected to ultrasonic-assisted extraction, removing solid residues to obtain a primary extract, transferring the primary extract into a supercritical fluid extraction device, selecting supercritical fluid as an extraction agent, and setting the temperature of the supercritical fluid to be 40 DEG C, the pressure to be 25MPa and the extraction time to be 60 minutes; and after the extraction is finished, collecting an extracting solution, removing residual solid particles through a filtering device, and concentrating the extracting solution by using a concentrating device to obtain a concentrated extract.

Description

Technical Field

[0001] This invention relates to the field of extraction technology of active ingredients from Phellinus linteus, and more specifically, to a method for preparing a concentrated extract of Phellinus linteus and a product thereof. Background Technology

[0002] Existing methods for preparing concentrated extracts of Phellinus linteus mainly include traditional methods such as water extraction and alcohol extraction. While these methods can achieve the extraction of active ingredients to a certain extent, they have the following significant drawbacks:

[0003] 1. Low extraction efficiency: Traditional methods usually rely on long soaking or heating, which results in slow release of active ingredients and low extraction efficiency;

[0004] 2. Low purity: Due to the inability to precisely control the conditions during the extraction process, impurities and ineffective components are easily mixed in, affecting the purity of the extract;

[0005] 3. High energy consumption: Traditional methods usually require a lot of energy to maintain the temperature and time during the extraction process, which increases production costs.

[0006] The preceding description is intended to provide general background information and does not necessarily constitute prior art. Summary of the Invention

[0007] The purpose of this invention is to provide a method for preparing concentrated extract of Phellinus linteus and a product thereof. This preparation method improves extraction efficiency and purity, reduces energy consumption, and provides new technical support for the quality control and market application of Phellinus linteus products.

[0008] This invention provides a method for preparing a concentrated extract of Phellinus linteus, comprising the following steps:

[0009] S1: Raw material pretreatment: Take an appropriate amount of dried Sanghuang powder, place it in a desiccator and dry it further to constant weight, remove residual moisture, and sieve the dried Sanghuang powder.

[0010] S2: Ultrasonic-assisted extraction: Add the pretreated Phellinus linteus powder to the extraction solvent at a mass ratio of 1:10, mix well, place the mixture in an ultrasonic extractor, set the ultrasonic frequency to 40kHz, the power to 200W, and the extraction time to 30 minutes.

[0011] S3: Supercritical fluid extraction: Filter the mixture after ultrasonic-assisted extraction to remove solid residues and obtain a preliminary extract. Transfer the preliminary extract to a supercritical fluid extraction device, select supercritical fluid as the extractant, and set the temperature of the supercritical fluid to 40℃, the pressure to 25MPa, and the extraction time to 60 minutes.

[0012] S4: Collection and purification: After extraction, the extract is collected and residual solid particles are removed by filtration. The extract is then concentrated using a concentration device to obtain a concentrated extract, which is then analyzed using high performance liquid chromatography.

[0013] Furthermore, the extraction solvent in step S2 is 70% ethanol.

[0014] Furthermore, step S2 also includes: periodically stirring the mixture during the extraction process.

[0015] Furthermore, the supercritical fluid in step S3 is carbon dioxide.

[0016] The present invention also provides a concentrated extract product, which is prepared by the above-mentioned method for preparing concentrated extract of Phellinus linteus.

[0017] Furthermore, the concentrated extract product is in the form of capsules, tablets, or oral liquid.

[0018] The method for preparing concentrated extract of Phellinus linteus provided by this invention combines ultrasonic-assisted extraction and supercritical fluid extraction technologies, offering a new, efficient, and environmentally friendly method for extracting the effective components of Phellinus linteus. This method not only improves extraction efficiency and purity but also reduces energy consumption and costs, providing strong technical support for the quality control and market application of Phellinus linteus products. Attached Figure Description

[0019] Figure 1 This is a schematic flowchart of the preparation method of concentrated extract of Phellinus linteus provided in Example 1 of the present invention. Detailed Implementation

[0020] The specific embodiments of the present invention will be described in further detail below with reference to the accompanying drawings and examples. The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

[0021] The terms "first," "second," "third," "fourth," etc., used in the specification and claims of this invention are used to distinguish similar objects and are not necessarily used to describe a specific order or sequence.

[0022] Example 1

[0023] The raw materials and equipment used in this embodiment are as follows:

[0024] Raw material: dried Phellinus linteus powder (particle size ≤ 0.5 mm), purity ≥ 95%.

[0025] Equipment: Ultrasonic extractor (adjustable frequency, controllable power), supercritical fluid extraction device (including temperature and pressure control system), filtration device, concentration device, and detection instruments (such as high performance liquid chromatograph).

[0026] Figure 1 This is a schematic flowchart illustrating the preparation method of the concentrated extract of Phellinus linteus provided in Example 1 of the present invention. Please refer to... Figure 1 The method for preparing concentrated extract of Phellinus linteus provided in this embodiment of the invention includes the following steps:

[0027] S1: Raw material pretreatment: Take an appropriate amount of dried Sanghuang powder, place it in a desiccator and dry it further to constant weight to remove residual moisture. Then, sieve the dried Sanghuang powder to ensure uniform particle size and no impurities.

[0028] S2: Ultrasonic-assisted extraction: Add the pretreated Phellinus linteus powder to the extraction solvent at a mass ratio of 1:10, mix thoroughly, place the mixture in an ultrasonic extractor, set the ultrasonic frequency to 40kHz, the power to 200W, and the extraction time to 30 minutes; during the extraction process, stir the mixture periodically to ensure uniform ultrasonic action. The extraction solvent in step S2 is 70% ethanol.

[0029] S3: Supercritical fluid extraction: The mixture after ultrasonic-assisted extraction is filtered to remove solid residues and obtain a preliminary extract. The preliminary extract is transferred to a supercritical fluid extraction device, and supercritical fluid is selected as the extractant. The temperature of the supercritical fluid is set to 40°C, the pressure to 25 MPa, and the extraction time to 60 minutes. The supercritical fluid in step S3 is carbon dioxide.

[0030] It should be noted that during the extraction process, maintaining stable temperature and pressure is crucial to ensure extraction efficiency; the vibration of ultrasound can disrupt the cell walls of Phellinus linteus and promote the rapid release of active ingredients.

[0031] S4: Collection and Purification: After extraction, the extract is collected and residual solid particles are removed by filtration. The extract is then concentrated using a concentration device to obtain a concentrated extract. The concentrated extract is then tested using high-performance liquid chromatography to ensure that its purity and active ingredient content meet the standards.

[0032] The preparation method of the concentrated extract of Phellinus linteus of the present invention:

[0033] I. In-depth exploration of the physical mechanism: When ultrasound propagates in a liquid medium, the microjets and shock waves generated by its cavitation effect can precisely act on the microstructure of Phellinus linteus cells, especially the cell wall, a key barrier to the release of effective components; by precisely controlling the frequency and power of ultrasound, effective destruction of the cell wall is achieved without damaging the structure of the effective components, which is difficult to match by traditional mechanical or chemical methods.

[0034] II. Optimized control of dynamic process: In the process of ultrasonic-assisted extraction, the flow of solvent and the release of components are in a dynamic equilibrium. This invention achieves precise control of this dynamic process by real-time monitoring and adjustment of the action time and intensity of ultrasound, as well as the composition and temperature of the solvent, thereby maximizing the release efficiency and purity of the effective components.

[0035] III. Unique advantages of supercritical state: Supercritical fluids, especially supercritical carbon dioxide, have a density close to that of liquids and a diffusivity close to that of gases. This unique physical state allows it to efficiently penetrate into the tiny pores of Phellinus linteus and make full molecular-level contact with the active ingredients. This invention finds the optimal extraction conditions by precisely controlling the temperature and pressure of the supercritical fluid, and achieves efficient and selective extraction of the active ingredients.

[0036] IV. Environmentally Friendly Green Technology: Supercritical fluid extraction technology avoids the use of traditional organic solvents, reducing environmental pollution and subsequent processing costs; especially for precious and environmentally sensitive Chinese medicinal materials such as Sanghuang, the use of supercritical fluid extraction technology can better protect its natural components and ecological environment.

[0037] In summary, the method for preparing concentrated extract of Phellinus linteus of the present invention provides a new, efficient, and environmentally friendly method for extracting the effective components of Phellinus linteus by combining ultrasonic-assisted extraction and supercritical fluid extraction technologies. This method not only improves extraction efficiency and purity but also reduces energy consumption and costs, providing strong technical support for the quality control and market application of Phellinus linteus products.

[0038] Example 2

[0039] This embodiment provides a concentrated extract product, which is prepared using the method for preparing the concentrated extract of Phellinus linteus from Example 1. It should be noted that the concentrated extract product of this embodiment is in capsule, tablet, or oral liquid dosage form.

[0040] As can be seen from the above description, the advantages of this invention are:

[0041] 1. Dual improvement in extraction efficiency and purity: Through the assistance of ultrasound and the precise extraction of supercritical fluid, efficient and selective extraction of the effective components of Phellinus linteus is achieved; compared with traditional methods, the extraction efficiency is increased by several times or even dozens of times, while the purity is also significantly improved, providing high-quality raw materials for subsequent product development.

[0042] 2. Comprehensive quality control: Every key step in the extraction process is meticulously controlled and optimized, from raw material pretreatment to extraction condition setting, and then to extract purification and testing, achieving comprehensive quality control. This comprehensive quality control method ensures the stability and consistency of the extract, providing a strong guarantee for the market application of the product.

[0043] 3. Sustainable and environmentally friendly technology: Using supercritical fluid as the extractant avoids the use and emission of organic solvents, reducing environmental pollution; at the same time, ultrasonic-assisted extraction technology also reduces energy consumption and production costs, making the entire extraction process more environmentally friendly and sustainable.

[0044] 4. Innovative Technological Breakthrough: This invention not only provides a new method for extracting the effective components of Phellinus linteus, but also brings an innovative technological breakthrough to the field of extraction and processing of Chinese medicinal materials. This integrated approach provides valuable experience and ideas for the extraction and processing of other Chinese medicinal materials, promoting the modernization and innovative development of Chinese medicinal material processing technology.

[0045] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. A method for preparing a concentrated extract of Phellinus linteus, characterized in that, Includes the following steps: S1: Raw material pretreatment: Take an appropriate amount of dried Sanghuang powder, place it in a desiccator and dry it further to constant weight, remove residual moisture, and sieve the dried Sanghuang powder. S2: Ultrasonic-assisted extraction: Add the pretreated Phellinus linteus powder to the extraction solvent at a mass ratio of 1:10, mix well, place the mixture in an ultrasonic extractor, set the ultrasonic frequency to 40kHz, the power to 200W, and the extraction time to 30 minutes. S3: Supercritical fluid extraction: Filter the mixture after ultrasonic-assisted extraction to remove solid residues and obtain a preliminary extract. Transfer the preliminary extract to a supercritical fluid extraction device, select supercritical fluid as the extractant, and set the temperature of the supercritical fluid to 40℃, the pressure to 25MPa, and the extraction time to 60 minutes. S4: Collection and purification: After extraction, the extract is collected and residual solid particles are removed by filtration. The extract is then concentrated using a concentration device to obtain a concentrated extract, which is then analyzed using high performance liquid chromatography.

2. The method for preparing concentrated extract of Phellinus linteus according to claim 1, characterized in that, The extraction solvent in step S2 is 70% ethanol.

3. The method for preparing concentrated extract of Phellinus linteus according to claim 1, characterized in that, Step S2 further includes: stirring the mixture periodically during the extraction process.

4. The method for preparing concentrated extract of Phellinus linteus according to claim 1, characterized in that, The supercritical fluid in step S3 is carbon dioxide.

5. A concentrated extract product, characterized in that, The concentrated extract product is prepared using the method for preparing the concentrated extract of Phellinus linteus according to any one of claims 1-4.

6. The concentrated extract product according to claim 5, characterized in that, The concentrated extract product is available in capsule, tablet, or oral liquid form.

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