Application of halogenated alkane dehalogenase in selective preparation of S-configuration aryl ethanol
The preparation of S-configuration arylethanol from phenyl chlorides in methyl tert-butyl ether solvent by the dehalogenase DbjA of haloalkane solves the problems of insufficient product purity and complicated operation in the preparation of optically active alcohols in the prior art, and realizes a highly efficient and simplified enzyme-catalyzed preparation process.
CN121518591APending Publication Date: 2026-02-13ZHEJIANG UNIV OF TECH
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Patent Information
- Application Number
- CN202510298460.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-13
- Publication Date
- 2026-02-13
AI Technical Summary
Technical Problem
Existing technologies for preparing optically active alcohols suffer from problems such as insufficient product purity and complex operations, especially in enzyme-catalyzed resolution where modifications are required and costs are high.
Method used
The S-configuration arylethanol was prepared by using the haloalkane dehalogenase DbjA to catalyze the dehalogenation of phenyl chlorides, with the enzyme catalysis carried out in methyl tert-butyl ether solvent and ultrapure water as an auxiliary dispersant to optimize the enzyme catalytic system.
Benefits of technology
The preparation of S-configuration arylethanol with high enantioselectivity and high yield was achieved, with an enantioselectivity of over 500 and an ee value of up to 99%, simplifying the operation process.
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Abstract
The invention belongs to the technical field of enzyme engineering, and particularly relates to application of halogenated alkane dehalogenase in selective preparation of S-configuration aryl ethanol, the halogenated alkane dehalogenase is DbjA, and the amino acid sequence of the halogenated alkane dehalogenase is shown as SEQ ID NO.1. According to the invention, the halogenated alkane dehalogenase DbjA has stereoselectivity for the reaction of catalyzing phenyl chloride to prepare S-configuration aryl ethanol, the enantiomer selectivity for the catalytic reaction of different substrates can reach 500 or more, the ee value of the generated substrate can reach 99%, and meanwhile, the yield of 40% or more can be maintained; and a new solution is provided for the problems of complex operation and large workload in the preparation process of S-configuration aryl ethanol.
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