Pentaaminolevulinic acid preparation device and preparation method thereof

Abstract

The invention relates to the technical field of fermentation preparation, in particular to a penaminolevulinic acid preparation device and a preparation method thereof.The penaminolevulinic acid preparation device comprises a fermentation vat, a clamping block is rotationally installed at the bottom of an inner cavity of the fermentation vat through a motor, a plurality of arc plates are arranged on the outer side of the clamping block, a rotating rod is detachably installed at the top of the clamping block, and a fermentation frame is arranged on the outer side of the bottom of the rotating rod; through the detachable connection design of the rotating rod and the clamping block, the whole device can be conveniently taken out of the fermentation barrel, thorough cleaning of the interior of the fermentation barrel is facilitated, the fermentation frame is used for fixing the filter cloth, solid-liquid separation of fermentation liquid is effectively achieved, and the stirring unit can conduct basic mixing and can also conduct stirring. A uniform, stable and efficient environment is created for microbial reaction, the arranged oxygen supply assembly works cooperatively with the adjusting assembly and the stirring unit, and oxygen is supplied synchronously in the stirring process, so that the gas-liquid contact area is remarkably increased, and the oxygen dissolution efficiency and the thallus utilization rate are improved.

Description

Technical Field

[0001] This invention relates to the field of fermentation preparation technology, specifically to a pentaminolevulinic acid preparation apparatus and preparation method. Background Technology

[0002] The preparation of pentaaminolevulinic acid, especially through microbial fermentation, is a complex process. Fermentation for pentaaminolevulinic acid preparation is a highly aerobic process, requiring a large amount of oxygen for both cell growth and product synthesis. During fermentation, especially with a carbon source, adding too high a concentration at once will not only inhibit cell growth, waste raw materials, and suppress the synthesis of the target product, but also cause the cells to precipitate to the bottom of the tank or adhere to the tank walls and sensors if the fermentation broth viscosity is too high or the stirring efficiency is insufficient. Cells in these areas will not be able to obtain nutrients and oxygen, and will die and autolyze, which will not only reduce the yield but also release impurities and increase the burden on subsequent extraction.

[0003] The prior art discloses a Chinese patent with application number CN202222285501.1, which discloses a high-efficiency separation device for aminolevulinic acid. It discloses the cooperation between the connecting rod and the movable ring, as well as the spring and the filter screen, which can filter impurities in the solution. Through the cooperation between the disc and the second hemisphere, and the first hemisphere and the top plate, the movable ring can drive the filter screen to vibrate, thereby preventing the filter screen from clogging. Through the cooperation between the motor and the rotating shaft, and the first sleeve and the first stirring rod, the solution before filtration can be stirred. Through the cooperation between the second sleeve and the second stirring rod, the solution after filtration can be stirred again, thereby facilitating the thorough mixing of crude aminolevulinic acid and concentrated hydrochloric acid.

[0004] Although the above-mentioned device can achieve stirring of the solution during the preparation of p-pentaaminolevulinic acid, it still has some drawbacks in use: 1. When the above device stirs the fermentation solution, it can perform single circumferential stirring along the axis of the stirring rod. The fermentation liquid rotates around the stirring axis under the action of centrifugal force. However, the fluid velocity is very low in the areas near the tank wall, bottom corners, liquid surface and between the stirring paddles, forming "dead zones". 2. Stirring and aeration are the two most energy-consuming steps in the fermentation process of pentaaminolevulinic acid preparation. In other words, the fermentation broth needs sufficient oxygen, but the oxygen in the fermenter is often difficult to fully enter the fermentation broth, thus causing fermentation failure and resulting in a decrease in yield. Summary of the Invention

[0005] The purpose of this invention is to provide a device and method for preparing pentaminolevulinic acid, so as to solve the problem mentioned in the background art of avoiding the reduction in yield of pentaminolevulinic acid due to insufficient stirring during fermentation.

[0006] The objective of this invention can be achieved through the following technical solutions: A device for preparing pentaminolevulinic acid includes a fermentation tank and a cover plate on top of the fermentation tank. A locking block is installed at the bottom of the inner cavity of the fermentation tank by a motor. Multiple arc plates are provided on the outer side of the locking block. A rotating rod is detachably installed on the top of the locking block. A fermentation frame is provided on the outer side of the bottom of the rotating rod. A filter cloth for solid-liquid separation during the fermentation of pentaminolevulinic acid is placed in the fermentation frame. The rotating rod is also equipped with a stirring unit to make the pentaminolevulinic acid ferment evenly. The stirring unit is equipped with an adjustment component that continuously changes the stirring range. The stirring unit is also equipped with an oxygen supply component that supplies oxygen required for the fermentation of pentaminolevulinic acid.

[0007] Preferably, a storage box for placing carbon source is provided in the middle of the rotating rod. A cylinder is installed between the top of the upper end face of the storage box and the rotating rod. A slide rod located inside the rotating rod is fixedly installed in the middle of the lower end face of the storage box. A cross groove arranged in a circle is opened inside the rotating rod. An internal strip fixedly connected to the slide rod is slidably installed in the cross groove.

[0008] Preferably, a rectangular groove is provided in the middle of the rotating rod, and a movable ring is slidably installed in multiple rectangular grooves. Each of the multiple rectangular grooves is provided with a spring connected to the bottom of the movable ring. Multiple circumferentially arranged striking rods are fixedly installed on the top of the movable ring. The top of the striking rods is provided with a cone-shaped plug, which blocks the discharge port at the bottom of the storage box.

[0009] Preferably, the agitation unit includes a wing plate installed outside the rotating rod and arranged in a circumferential direction. A guide groove is provided in the middle of the wing plate, and a moving block is slidably installed in the guide groove. An inner rod is fixedly installed at the lower end of the moving block, and an outer rod is slidably installed at the lower part of the inner rod. An oxygen supply hole is provided in a circumferential direction on the lower side of the outer rod, and a roller is provided at the bottom of the outer rod.

[0010] Preferably, the oxygen supply assembly includes an air cylinder one disposed on the side of the inner rod away from the rotating rod, the inner cavity of the air cylinder one being connected to the center of the inner rod, a piston rod one being slidably mounted in the middle of the air cylinder one, and a fixing member fixedly mounted at one end of the piston rod one and fixedly connected to the wing plate.

[0011] Preferably, the adjustment assembly includes a rotating plate that is rotatably connected to the rotating rod via an electric slider. The rotating plate has multiple circumferentially arranged arc-shaped grooves. A shaft is slidably installed in the arc-shaped grooves. A push plate is provided at the bottom of the shaft. A sleeve plate located outside the inner rod is rotatably installed on one side of the push plate.

[0012] Preferably, an air cylinder two is fixedly installed on one side of the fermentation tank, a piston rod two is slidably installed in the middle of the air cylinder two, a bevel gear one is fixedly installed on the top of the rotating rod, a support frame fixed to the fermentation tank is provided on one side of the bevel gear one, an electric push rod is rotatably installed in the middle of the support frame through a bearing, a bevel gear two is fixedly installed at one end of the electric push rod, and a cam is fixedly installed at the end of the electric push rod away from the bevel gear two, and a round shaft is provided on the cam and inserted into the inclined groove opened at the top of the piston rod two.

[0013] Preferably, one side of the second air cylinder is provided with a circumferential pipe fixed to the fermentation tank. The circumferential pipe is connected to the inside of the second air cylinder. Multiple circumferentially arranged injection pipes are fixedly installed on the circumferential pipe. The end of the injection pipe away from the circumferential pipe is inserted into the fermentation tank and the bottom is threaded and detachably fitted with a vent cover. The vent cover is provided with a filter screen located inside the injection pipe.

[0014] Preferably, the cover plate is assembled at both ends, and the middle of the cover plate is semi-circular, with a groove on the edge of the cover plate corresponding to the injection pipe.

[0015] Another object of the present invention is to provide a method for preparing a pentaaminolevulinic acid preparation apparatus, comprising the following steps: S1: Fermentation preparation: The raw materials required for the fermentation of pentaaminolevulinic acid are placed in the fermentation tank and the fermentation tank is turned on to maintain a suitable temperature. The carbon source in the storage tank is continuously added to increase the yield. S2: Fermentation stirring: By turning on the stirring unit, the fermentation preparation of pentaaminolevulinic acid can be continuously stirred so that the carbon source added later can be quickly dispersed, avoiding excessively high local concentrations. Stirring also helps to transfer heat and keep the fermentation broth consistent. S3: Oxygen Injection Due to the aerobic nature of the strains used in the fermentation of pentaaminolevulinic acid, oxygen needs to be injected during fermentation, and an oxygen supply component facilitates a sufficient supply of oxygen.

[0016] The beneficial effects of this invention are: 1. The present invention uses a detachable connection design between the rotating rod and the snap-fit ​​block, which allows the entire device to be easily removed from the fermentation tank, which is conducive to thorough cleaning of the inside of the fermentation tank. The fermentation frame is used to fix the filter cloth, which effectively realizes the solid-liquid separation of the fermentation liquid. The stirring unit can not only perform basic mixing, but also create a uniform, stable and efficient environment for microbial reaction.

[0017] 2. This invention effectively expands the mixing area within the fermentation tank through dual stirring actions of circumferential and horizontal displacement, eliminating the mixing dead zones present in traditional single-rotation stirring. Newly added materials can be quickly and evenly dispersed throughout the fermentation tank, avoiding localized uneven concentrations and improving the consistency of the fermentation system. Dynamic stirring enhances the overall fluidity of the fermentation broth, especially improving the mass transfer efficiency in traditional low-speed areas (such as near the wall, bottom, and liquid surface corners), ensuring sufficient contact between oxygen and the cells. Compared to traditional circumferential stirring, this invention optimizes oxygen transfer and distribution through multi-directional dynamic mixing, thereby potentially improving the fermentation efficiency and product quality of pentaminolevulinic acid.

[0018] 3. The oxygen supply component, adjustment component, and stirring unit of this invention work together to supply oxygen synchronously during the stirring process. By using the high-speed rotating outer rod to shear oxygen and form micron-sized bubbles, the gas-liquid contact area is significantly increased, the bubble residence time is extended, and the oxygen dissolution efficiency and cell utilization rate are improved. At the same time, oxygen is evenly distributed in the fermenter. This integrated design combines stirring and oxygen supply functions, and automatically triggers oxygen supply while adjusting the stirring range, which greatly reduces energy consumption and production costs.

[0019] 4. In this invention, as the storage box moves up and down, the spring drives the striking rod and the blocking block to vibrate at high frequency, continuously striking the material inside the box and promoting its smooth descent. When the storage box rises to a specific height, the striking rod and the blocking block reach their upper limit. As the storage box continues to move upward, the discharge port is opened, enabling controllable material discharge. The rotating rod drives multiple outer rods to rotate around the axis, achieving preliminary stirring of the material. The rollers at the bottom of the outer rods intermittently contact the arc plate, driving the outer rods to move up and down along the inner rods, forming a compound rotation and lifting stirring motion, which significantly enhances the mixing intensity of the fermented material and improves the stirring efficiency and uniformity of the preparation of pentaaminolevulinic acid.

[0020] 5. This invention uses an electric slider to drive a rotating plate to rotate alternately in both directions, causing multiple inner rods to converge or separate, thus flexibly adjusting the stirring range. Simultaneously, the movement of the inner rods drives the piston rod inside the first gas cylinder to reciprocate, pressing oxygen through the inner rods into the outer rods and injecting it unidirectionally into the fermentation broth through the oxygen supply hole. This effectively prevents liquid backflow, achieving efficient and safe coordination between stirring and oxygen supply. An electric push rod drives a second bevel gear to mesh with a first bevel gear, causing the rotating rod to rotate and drive a cam to push the second piston rod, compressing external oxygen and injecting it into the inlet pipe. The filter effectively filters impurities in the oxygen, ensuring the airtightness of the fermentation tank while achieving clean oxygen injection, thus balancing a sterile environment and safe gas supply. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, for those skilled in the art, other drawings can be obtained based on these drawings without creative effort. Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 This is a partial top view of the structure of the present invention; Figure 3 This is a schematic diagram of the orthographic structure of the present invention; Figure 4 This is the present invention. Figure 3 A magnified structural diagram of part A; Figure 5 This is a schematic diagram of the stirring unit structure of the present invention; Figure 6 This is the present invention. Figure 5 A schematic diagram of the enlarged structure of part B; Figure 7 This is a schematic diagram of the structure between the fermentation frame and the rotating rod of the present invention; Figure 8 This is a schematic diagram of the structure between the fermentation frame and the snap-fit ​​block of the present invention; Figure 9 This is a top-section structural diagram of the sliding rod and rotating rod of the present invention.

[0022] The attached figures are labeled as follows: 1. Fermentation tank; 102. Cover plate; 10. Clip-on block; 11. Arc plate; 12. Rotating rod; 121. Cross groove; 122. Sliding rod; 123. Internal strip; 13. Storage box; 14. Cylinder; 151. Rectangular groove; 152. Moving ring; 153. Striking rod; 16. Fermentation frame; 162. Filter cloth; 2. Wing plate; 20. Guide groove; 21. Moving block; 22. Inner rod; 23. Outer rod ; 24. Rotating plate; 241. Arc groove; 242. Shaft; 243. Push plate; 244. Sleeve plate; 25. Air cylinder one; 251. Piston rod one; 252. Fixing component; 3. Air cylinder two; 31. Piston rod two; 32. Circular pipe; 33. Inlet pipe; 34. Vent cover; 35. Filter screen; 4. Bevel gear one; 41. Support frame; 42. Electric push rod; 43. Bevel gear two; 44. Cam. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] As attached Figure 1-9 As shown, a pentaminolevulinic acid preparation device includes a fermentation tank 1 and a cover plate 102 on the top of the fermentation tank 1. A snap-fit ​​block 10 is installed at the bottom of the inner cavity of the fermentation tank 1 via a motor. Multiple arc plates 11 are provided on the outer side of the snap-fit ​​block 10. A rotating rod 12 is detachably installed on the top of the snap-fit ​​block 10. A fermentation frame 16 is provided on the outer side of the bottom of the rotating rod 12. A filter cloth 162 for solid-liquid separation during the fermentation of pentaminolevulinic acid is placed in the fermentation frame 16. A stirring unit is also provided on the rotating rod 12 to make the pentaminolevulinic acid ferment evenly. An adjustment component for continuously changing the stirring range is provided in the stirring unit. An oxygen supply component for supplying oxygen required during the fermentation of pentaminolevulinic acid is also provided in the stirring unit. The rotating rod 12 can be disassembled from the snap-fit ​​block 10, allowing the entire device on the rotating rod 12 to be simultaneously removed from the fermentation tank 1, facilitating cleaning of the fermentation tank 1. The fermentation frame 16 allows for the installation of the filter cloth 162, which facilitates solid-liquid separation of the fermentation broth. The stirring unit not only performs simple mixing during the preparation of pentaminolevulinic acid fermentation but also creates and maintains a uniform, stable, and efficient reaction environment for microbial cells. This is reflected in increasing and maintaining dissolved oxygen levels, allowing the microbial community to grow vigorously under high dissolved oxygen conditions during pentaminolevulinic acid fermentation, thus creating a good aerobic environment for pentaminolevulinic acid fermentation. During stirring, the large bubbles during fermentation are broken into countless small bubbles, greatly increasing the contact area between the gas and liquid phases. This allows oxygen to dissolve into the liquid more quickly, ensuring a consistent dissolved oxygen concentration within fermentation tank 1. This effectively prevents severe inhibition of cell growth and product development due to inconsistent dissolved oxygen levels within fermentation tank 1. On the other hand, the stirring unit maintains cell suspension during the fermentation of pentaaminolevulinic acid, preventing cells from settling to the bottom of fermentation tank 1 due to gravity. This effectively avoids cell death and autolysis caused by cell settling, which would reduce effective biomass and yield. Furthermore, autolysis of cells produces a large number of impurities (such as proteins and nucleic acids), making subsequent purification difficult. By adjusting the component settings, when the stirring unit is stirring the fermentation preparation of pentaminolevulinic acid, it can simultaneously perform circumferential stirring and horizontal stirring, achieving dynamic mixing. This expands the stirring action within the fermentation tank 1, and ensures that newly added materials are evenly dispersed within the fermentation tank 1. Compared to existing devices, this invention not only performs rotational stirring during the fermentation preparation of pentaminolevulinic acid but also achieves horizontal displacement stirring. In traditional circumferential stirring within the fermentation tank 1, the liquid flow rate remains low in some areas (such as near the inner wall, bottom, liquid corners, and areas between the stirring paddles), making stirring difficult. Consequently, oxygen and cell exchange is often impaired. Dynamic stirring, on the other hand, covers the entire fermentation tank 1, ensuring that all cells receive sufficient oxygen and preventing metabolic abnormalities or death caused by localized anaerobic conditions. The oxygen supply component is designed to inject oxygen into the raw material by adjusting the stirring range of the stirring unit through oxygen supply holes on the outer rod 23. When oxygen is released directly from the oxygen supply holes at the end and edge of the high-speed rotating outer rod 23, it is instantly pulverized into extremely small bubbles by the huge shear force generated by the outer rod 23. The smaller the bubbles, the larger the total gas-liquid contact area. The smaller the bubbles, the slower their rising speed, and the longer their residence time in the fermentation broth. This allows the oxygen more time to dissolve and be utilized by the microorganisms. The oxygen is released from the air cylinder 25 and is then carried into the strong flow field generated by the outer rod 23. Subsequently, it is immediately dispersed throughout the tank through the oxygen supply holes, avoiding the problem of uneven oxygen distribution that may occur in traditional methods. Compared with existing devices, this invention integrates stirring and oxygen supply. The oxygen supply component is activated by the stirring unit driven by the adjusting component to control the stirring range. Since stirring and aeration are the biggest energy consumption processes in fermentation, the design adopted in this invention can significantly reduce energy consumption, save energy, and reduce production costs.

[0025] The rotating rod 12 has a storage box 13 for placing carbon source in the middle. A cylinder 14 is installed between the top of the upper end face of the storage box 13 and the rotating rod 12. A slide rod 122 located inside the rotating rod 12 is fixedly installed in the middle of the lower end face of the storage box 13. A cross groove 121 arranged in a circle is opened inside the rotating rod 12. An inner strip 123 fixedly connected to the slide rod 122 is slidably installed in the cross groove 121. With the cross groove 121 and the built-in bar 123, the storage box 13 can rotate with the rotating rod 12, and the cylinder 14 can also extend and retract to drive the storage box 13 to move up and down. When the storage box 13 moves upward, it can break free from the blockage of the discharge port at the bottom of the storage box 13, so that the carbon source material in the storage box 13 can be replenished at irregular intervals. It should be noted that: as the core additive in the fermentation preparation of pentamirrylpropionic acid, adding too high a concentration of carbon source at one time will inhibit the growth of cells. Therefore, it is necessary to continuously replenish the carbon source during the fermentation preparation to prevent it from being depleted and causing the fermentation to end prematurely.

[0026] A rectangular groove 151 is circumferentially opened in the middle of the rotating rod 12. A moving ring 152 is slidably installed in multiple rectangular grooves 151. A spring connected to the bottom of the moving ring 152 is provided in each of the multiple rectangular grooves 151. Multiple circumferentially arranged striking rods 153 are fixedly installed on the top of the moving ring 152. A cone-shaped plug is provided on the top of the striking rod 153. The plug seals the discharge port opened at the bottom of the storage box 13. As the storage box 13 moves up and down, the spring causes the striking rod 153 and the blockage to vibrate up and down, thereby striking the material inside the storage box 13 to facilitate better material discharge. When the storage box 13 moves up to a certain position, the striking rod 153 and the blockage reach their highest position. As the storage box 13 continues to move up, the discharge port is unblocked, allowing the material to fall.

[0027] The stirring unit includes a wing plate 2 installed outside the rotating rod 12 and arranged in a circumferential direction. A guide groove 20 is opened in the middle of the wing plate 2. A moving block 21 is slidably installed in the guide groove 20. An inner rod 22 is fixedly installed at the lower end of the moving block 21. An outer rod 23 is slidably installed at the lower part of the inner rod 22. An oxygen supply hole is opened in a circumferential direction on the lower side of the outer rod 23. A roller is provided at the bottom of the outer rod 23. When the stirring unit stirs the fermentation tank 1, the motor is turned on and drives the rotating rod 12 to rotate through the snap-fit ​​block 10. When the rotating rod 12 rotates, it drives multiple outer rods 23 to rotate along the axis of the rotating rod 12, thereby stirring the fermentation material during the preparation of pentaaminolevulinic acid. When the outer rods 23 rotate, the rollers at the bottom of the outer rods 23 intermittently contact the arc plate 11 at the bottom of the fermentation tank 1, thereby causing the outer rods 23 to move up and down on the inner rods 22. Through the up and down movement of the outer rods 23, the outer rods 23 can stir up and down at the same time as they rotate in a circle, further enhancing the stirring effect during the preparation of pentaaminolevulinic acid.

[0028] The oxygen supply assembly includes an air cylinder 25 disposed on the side of the inner rod 22 away from the rotating rod 12. The inner cavity of the air cylinder 25 is connected to the center of the inner rod 22. A piston rod 251 is slidably installed in the middle of the air cylinder 25. A fastener 252 that is fixedly connected to the wing plate 2 is fixedly installed at one end of the piston rod 251.

[0029] The adjustment assembly includes a rotating plate 24 that is rotatably connected to the rotating rod 12 via an electric slider. The rotating plate 24 has multiple circumferentially arranged arc-shaped grooves 241. A shaft 242 is slidably installed in the arc-shaped grooves 241. A push plate 243 is provided at the bottom of the shaft 242. A sleeve plate 244 located outside the inner rod 22 is rotatably installed on one side of the push plate 243. The electric slider opens and drives the rotating plate 24 to rotate clockwise and counterclockwise alternately on the rotating rod 12, so that the multiple inner rods 22 rotate closer or further apart. When the inner rods 22 move, the air cylinder 25 approaches the fixed part 252. At this time, the piston rod 251 reciprocates and compresses inside the air cylinder 25, thereby transmitting oxygen through the inner rods 22 to the outer rod 23, and finally through the oxygen supply hole on the inner rod 22 to the inside of the fermentation liquid. Since the oxygen supply hole is equipped with a one-way valve, when the air cylinder 25 moves away from the piston rod 251, the liquid in the fermentation liquid will not flow back into the outer rod 23.

[0030] A second air cylinder 3 is fixedly installed on one side of the fermentation tank 1. A second piston rod 31 is slidably installed in the middle of the second air cylinder 3. A first bevel gear 4 is fixedly installed on the top of the rotating rod 12. A support frame 41 fixed to the fermentation tank 1 is provided on one side of the first bevel gear 4. An electric push rod 42 is rotatably installed in the middle of the support frame 41 through a bearing. A second bevel gear 43 is fixedly installed at one end of the electric push rod 42. A cam 44 is fixedly installed at the end of the electric push rod 42 away from the second bevel gear 43. A round shaft is provided on the cam 44 and inserted into the inclined groove opened at the top of the second piston rod 31. The electric push rod 42 is opened, causing the bevel gear 2 43 to mesh with the bevel gear 1 4. When the rotating rod 12 rotates, it can drive the electric push rod 42 to rotate, thereby causing the cam 44 to rotate and drive the piston rod 2 31 to compress in the air cylinder 2 3, thereby injecting external oxygen into the injection pipe 33. The oxygen can also resist impurities in the oxygen through the filter screen 35. While ensuring that the fermentation tank 1 is sealed, the injection of oxygen can also resist impurities in the oxygen.

[0031] One side of the air cylinder 3 is provided with a circumferential pipe 32 fixed to the fermentation tank 1. The circumferential pipe 32 is connected to the inside of the air cylinder 3. Multiple circumferentially arranged injection pipes 33 are fixedly installed on the circumferential pipe 32. The end of the injection pipe 33 away from the circumferential pipe 32 is inserted into the fermentation tank 1 and the bottom is threaded and detachably installed with a vent cover 34. The vent cover 34 is provided with a filter screen 35 located inside the injection pipe 33. By rotating the vent cover 34, it can be separated from the injection pipe 33, so as to facilitate the replacement of the filter screen 35.

[0032] The cover plate 102 is assembled at both ends, and the middle of the cover plate 102 is semi-circular. The edge of the cover plate 102 is provided with a groove corresponding to the injection pipe 33. The cover plate 102 is designed to facilitate removal from the fermentation tank 1.

[0033] Another object of the present invention is to provide a method for preparing a pentaaminolevulinic acid preparation apparatus, comprising the following steps: S1: Fermentation preparation: The raw materials required for the fermentation of pentaaminolevulinic acid are placed in fermentation tank 1 and fermentation tank 1 is turned on to maintain a suitable temperature. The carbon source in storage tank 13 is continuously added to increase the yield. S2: Fermentation stirring: By turning on the stirring unit, the fermentation preparation of pentaaminolevulinic acid can be continuously stirred so that the carbon source added later can be quickly dispersed, avoiding excessively high local concentrations. Stirring also helps to transfer heat and keep the fermentation broth consistent. S3: Oxygen Injection Due to the aerobic nature of the strains used in the fermentation of pentaaminolevulinic acid, oxygen needs to be injected during fermentation, and an oxygen supply component facilitates a sufficient supply of oxygen.

[0034] The working principle of this invention is as follows: First, the raw materials required for the fermentation of pentaminolevulinic acid are placed into the filter cloth 162 inside the fermentation frame 16. Then, the cover plate 102 is placed on the fermentation tank 1. Since the fermentation tank 1 can be opened for heating, the fermentation tank 1 is opened to maintain the temperature required for the fermentation of pentaminolevulinic acid. Heating of the fermentation tank 1 is existing technology and will not be described in detail. Then, the motor is turned on to drive the rotating rod 12 to rotate. When the rotating rod 12 rotates, it drives the stirring unit to mix the pentaminolevulinic acid during fermentation. At the same time, it can create and maintain a uniform, stable and efficient reaction environment for microbial cells, which is reflected in improving and maintaining dissolved oxygen water. On the flat surface, during the fermentation of pentaminolevulinic acid (PVA), the internal microbial community thrives under high dissolved oxygen conditions, creating an excellent aerobic environment for PVA fermentation. Stirring breaks down large bubbles during fermentation into numerous smaller bubbles, significantly increasing the contact area between the gas and liquid phases. This allows oxygen to dissolve into the liquid more quickly, ensuring a consistent dissolved oxygen concentration within fermentation tank 1. This effectively prevents severe inhibition of microbial growth and product development due to inconsistent dissolved oxygen levels. Furthermore, the stirring unit maintains the suspension of the microbial cells during the fermentation process, preventing them from settling to the bottom of fermentation tank 1 due to gravity. This effectively avoids the inability of settled microbial cells to interact with oxygen and nutrients. Contact causes bacterial death and autolysis, leading to a decrease in effective biomass and yield. The cylinder 14 retracts, causing the storage tank 13 to move upwards. As the storage tank 13 moves upwards, it breaks free from the blockage at the bottom of the discharge port, allowing for intermittent replenishment of the carbon source material within. The electric slider activates, causing the rotating plate 24 to rotate clockwise and counterclockwise alternately on the rotating rod 12. This causes the multiple inner rods 22 to move closer to or further away from each other. When the inner rods 22 move, the air cylinder 25 approaches the fixed part 252. At this time, the piston rod 251 reciprocates and compresses within the air cylinder 25, thereby transferring oxygen through the inner rods 22 to the outer rod 23, ultimately... The oxygen is transmitted to the fermentation broth through the oxygen supply hole on the inner rod 22. Because the oxygen supply hole is equipped with a one-way valve, when the air cylinder 25 moves away from the piston rod 251, the liquid in the fermentation broth will not flow back into the outer rod 23. The electric push rod 42 is opened, so that the bevel gear 43 meshes with the bevel gear 4. When the rotating rod 12 rotates, it can drive the electric push rod 42 to rotate, so that when the cam 44 rotates, it drives the piston rod 31 to compress in the air cylinder 3, thereby injecting external oxygen into the injection pipe 33. The oxygen can also resist impurities in the oxygen through the filter screen 35. Rotate the vent cover 34 to separate it from the injection pipe 33, so as to facilitate the replacement of the filter screen 35.

[0035] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. A device for preparing pentaminolevulinic acid, comprising a fermentation tank (1) and a cover plate (102) on top of the fermentation tank (1), characterized in that, The fermentation tank (1) has a snap-fit ​​block (10) installed at the bottom of the inner cavity by a motor. Multiple arc plates (11) are provided on the outside of the snap-fit ​​block (10). A rotating rod (12) is detachably installed on the top of the snap-fit ​​block (10). A fermentation frame (16) is provided on the outside of the bottom of the rotating rod (12). A filter cloth (162) for solid-liquid separation during the fermentation of pentaminolevulinic acid is placed in the fermentation frame (16). A stirring unit is also provided on the rotating rod (12) to make the pentaminolevulinic acid ferment evenly. An adjustment component for continuously changing the stirring range is provided in the stirring unit. An oxygen supply component for supplying oxygen required during the fermentation of pentaminolevulinic acid is also provided in the stirring unit.

2. The apparatus for preparing pentaaminolevulinic acid according to claim 1, characterized in that, The rotating rod (12) has a storage box (13) for placing carbon source in the middle. A cylinder (14) is installed between the top of the upper end face of the storage box (13) and the rotating rod (12). A slide rod (121) located inside the rotating rod (12) is fixedly installed in the middle of the lower end face of the storage box (13). A cross groove (122) arranged in a ring is opened inside the rotating rod (12). An internal strip (123) fixedly connected to the slide rod (121) is slidably installed in the cross groove (122).

3. The apparatus for preparing pentaaminolevulinic acid according to claim 2, characterized in that, The rotating rod (12) has a rectangular groove (151) circumferentially opened in the middle. A moving ring (152) is slidably installed in multiple rectangular grooves (151). A spring connected to the bottom of the moving ring (152) is provided in each of the multiple rectangular grooves (151). Multiple circumferentially arranged striking rods (153) are fixedly installed on the top of the moving ring (152). A cone-shaped plug is provided on the top of the striking rod (153). The plug seals the discharge port opened at the bottom of the storage box (13).

4. The apparatus for preparing pentaaminolevulinic acid according to claim 1, characterized in that, The stirring unit includes a wing plate (2) installed outside the rotating rod (12) and arranged in a circumferential direction. A guide groove (20) is provided in the middle of the wing plate (2). A moving block (21) is slidably installed in the guide groove (20). An inner rod (22) is fixedly installed at the lower end of the moving block (21). An outer rod (23) is slidably installed at the lower part of the inner rod (22). An oxygen supply hole is provided in a circumferential direction on the lower side of the outer rod (23). A roller is provided at the bottom of the outer rod (23).

5. The apparatus for preparing pentaaminolevulinic acid according to claim 1, characterized in that, The oxygen supply assembly includes an air cylinder (25) located on the side of the inner rod (22) away from the rotating rod (12). The inner cavity of the air cylinder (25) is connected to the center of the inner rod (22). A piston rod (251) is slidably installed in the middle of the air cylinder (25). A fixing member (252) that is fixedly connected to the wing plate (2) is fixedly installed at one end of the piston rod (251).

6. The apparatus for preparing pentaaminolevulinic acid according to claim 5, characterized in that, The adjustment assembly includes a rotating plate (24) that is rotatably connected to the rotating rod (12) via an electric slider. The rotating plate (24) has multiple circumferentially arranged arc grooves (241). A shaft (242) is slidably installed in the arc grooves (241). A push plate (243) is provided at the bottom of the shaft (242). A sleeve plate (244) located outside the inner rod (22) is rotatably installed on one side of the push plate (243).

7. The apparatus for preparing pentaaminolevulinic acid according to claim 1, characterized in that, A second air cylinder (3) is fixedly installed on one side of the fermentation tank (1). A second piston rod (31) is slidably installed in the middle of the second air cylinder (3). A first bevel gear (4) is fixedly installed on the top of the rotating rod (12). A support frame (41) fixed to the fermentation tank (1) is provided on one side of the first bevel gear (4). An electric push rod (42) is rotatably installed in the middle of the support frame (41) through a bearing. A second bevel gear (43) is fixedly installed at one end of the electric push rod (42). A cam (44) is fixedly installed at the end of the electric push rod (42) away from the second bevel gear (43). A round shaft is set on the cam (44) and inserted into the inclined groove opened at the top of the second piston rod (31).

8. The apparatus for preparing pentaaminolevulinic acid according to claim 7, characterized in that, The second gas cylinder (3) is provided with a circumferential pipe (32) fixed to the fermentation tank (1) on one side. The circumferential pipe (32) is connected to the inside of the second gas cylinder (3). Multiple circumferentially arranged injection pipes (33) are fixedly installed on the circumferential pipe (32). The end of the injection pipe (33) away from the circumferential pipe (32) is inserted into the fermentation tank (1) and the bottom is threaded and detachably fitted with a vent cover (34). The vent cover (34) is provided with a filter screen (35) located inside the injection pipe (33).

9. The apparatus for preparing pentaaminolevulinic acid according to claim 1, characterized in that, The cover plate (102) is assembled at both ends, and the middle part of the cover plate (102) is semi-circular. The edge of the cover plate (102) is provided with a groove corresponding to the injection pipe (33).

10. A method for preparing a pentaaminolevulinic acid preparation apparatus, applied to the preparation apparatus described in claim 1, characterized in that, Includes the following steps: S1: Fermentation preparation: The raw materials required for the fermentation preparation of pentaminolevulinic acid are placed in the fermentation tank (1) and the fermentation tank (1) is opened to maintain a suitable temperature. The carbon source in the storage tank (13) is continuously added to increase the yield. S2: Fermentation stirring: By turning on the stirring unit, the fermentation preparation of pentaaminolevulinic acid can be continuously stirred so that the carbon source added later can be quickly dispersed, avoiding excessively high local concentrations. Stirring also helps to transfer heat and keep the fermentation broth consistent. S3: Oxygen Injection Due to the aerobic nature of the strains used in the fermentation of pentaaminolevulinic acid, oxygen needs to be injected during fermentation, and an oxygen supply component facilitates a sufficient supply of oxygen.

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