Soothing and repairing composition as well as preparation method and application thereof

Abstract

The invention provides a soothing and repairing composition as well as a preparation method and application thereof. The soothing and repairing composition is prepared from ceramide, phytosterol, superoxide dismutase, cyclodextrin, a plant extract and water, the plant extract comprises any one or a combination of at least two of a glycyrrhiza inflata root extract, a glycyrrhiza glabra extract and a gynura divaricata root juice. The ceramide, the phytosterol, the superoxide dismutase and the plant extract in the composition are matched for use, so that the composition has excellent repairing, relieving, anti-inflammatory and anti-oxidation effects, in addition, the stability and the transdermal property of the active ingredients can be improved by using the cyclodextrin in the composition, and the composition can be used for repairing and relieving inflammation and resisting oxidation. And the skin feeling of the product can be further adjusted.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a soothing and repairing composition, its preparation method, and its application. Background Technology

[0002] The skin barrier, composed of the stratum corneum, sebum film, and intercellular lipids, directly determines skin tolerance. When the barrier is damaged, stratum corneum lipids are lost, intercellular connections loosen, and external irritants easily penetrate, triggering inflammatory responses. Simple moisturizing or anti-inflammatory methods are insufficient to address both the symptoms and the root cause. Therefore, cosmetic compositions that simultaneously achieve anti-inflammatory soothing, promote barrier regeneration, and enhance tolerance have become a core focus of industry technological research and development.

[0003] CN120643479A discloses a composition with soothing effects, its preparation method, and its application in cosmetics. The composition comprises the following components in the indicated weight percentages: 35-50% *Actinidia chinensis* fruit juice, 2-10% *Gentiana scabra* root extract, 1-9% *Citrus medica* fruit extract, 1-4% *Dendrobium nobile* stem extract, 1-5% oat extract, and the balance being butylene glycol. The components in this composition work synergistically to exert multi-dimensional synergistic effects, including anti-inflammatory, antihistamine, barrier repair, antioxidant, and microecological regulation, resulting in excellent soothing efficacy.

[0004] CN118662402A discloses a soothing face cream and its preparation method. This soothing face cream combines seven plant extracts, multiple ceramides, multiple natural plant oils, and tetrahydromethylpyrimidine carboxylic acid. The four core components are coordinated to create the soothing face cream, which, along with the synergistic effects of moisturizing, anti-aging, antibacterial, and anti-inflammatory components, provides long-lasting skin hydration, reduces dryness, flaking, and roughness, strengthens the skin barrier and aids in repair, enhances skin tolerance, and also has analgesic, sedative, anti-inflammatory, bactericidal, anti-inflammatory, and soothing effects. It can activate epidermal cells and promote healing and anti-inflammation. Simultaneously, it better regulates the skin's water-oil balance, providing a gentler and more comprehensive soothing effect. The preparation method of this soothing face cream is simple, uses mild preparation conditions, has high production efficiency, and is suitable for large-scale production.

[0005] However, while there are more and more soothing, anti-inflammatory and repairing products available, most of them have problems with the compatibility and synergistic mechanisms between ingredients, resulting in poor cumulative effects or even antagonism. Furthermore, due to uneven dispersion of ingredients, the absorption efficiency of local skin varies greatly, and the phenomenon of "effective local soothing but no local problem" may occur. Therefore, there is an urgent need to provide a product with significant and comprehensive soothing and repairing effects, as well as lower irritation and higher safety to meet the application needs. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a soothing and repairing composition, its preparation method, and its application.

[0007] To achieve this objective, the present invention adopts the following technical solution:

[0008] In a first aspect, the present invention provides a soothing and repairing composition comprising ceramide, phytosterol, superoxide dismutase, cyclodextrin, plant extract and water;

[0009] The plant extracts include any one or a combination of at least two of the following: Glycyrrhiza uralensis root extract, Glycyrrhiza glabra root extract, or Ipomoea purpurea root juice.

[0010] The ceramides described in this invention, as a core component of the intercellular lipids of the skin barrier, can directly replenish barrier lipids, maintain the integrity of the intercellular lipid bilayer structure of the stratum corneum, thicken the stratum corneum, maintain and repair the skin barrier, reduce the penetration of external irritants, and enhance the skin's anti-sensitivity; they also increase skin moisture content, reduce transepidermal water loss, and play an important moisturizing role; phytosterols, with a structure similar to cholesterol, can penetrate into the stratum corneum and synergistically enhance lipid barrier stability with ceramides, reducing water loss, while simultaneously exerting anti-inflammatory effects by inhibiting the release of inflammatory mediators, reducing redness and itching, making them suitable for sensitive skin and those experiencing photoaging. Skin repair; Superoxide dismutase (SOD), as an antioxidant enzyme, can specifically catalyze the conversion of superoxide anion free radicals into hydrogen peroxide and oxygen, reducing free radical-mediated inflammatory damage and barrier disruption, blocking free radical attacks on cell membranes, DNA, and collagen, reducing pigmentation and inflammation, alleviating oxidative damage, and achieving synergistic anti-inflammatory and antioxidant effects; when used in combination with plant extracts, the composition can have excellent repairing, soothing, anti-inflammatory, and antioxidant effects. In addition, the use of cyclodextrin in the composition of this invention can improve the stability and transdermal permeability of the active ingredients, and can further regulate the skin feel of the product.

[0011] Preferably, the soothing and repairing composition comprises, by weight percentage, 0.02%-0.15% ceramide, 0.01%-0.15% phytosterol, 0.4-10 ppm superoxide dismutase, 5%-15% cyclodextrin, 1%-10% plant extract and 70-95% water.

[0012] The percentage content of the ceramide in the soothing and repairing composition of the present invention can be 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, or 0.14%, etc.

[0013] The percentage content of the phytosterols can be 0.02%, 0.03%, 0.04%, 0.05%, 0.06%, 0.07%, 0.08%, 0.09%, 0.1%, 0.11%, 0.12%, 0.13%, or 0.14%, etc.;

[0014] The content of the superoxide dismutase can be 0.5 ppm, 0.8 ppm, 1 ppm, 2 ppm, 3 ppm, 4 ppm, 5 ppm, 6 ppm, 7 ppm, 8 ppm or 9 ppm, etc.;

[0015] The percentage content of the cyclodextrin can be 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13% or 14%, etc.

[0016] The percentage content of the plant extract can be 2%, 3%, 4%, 5%, 6%, 7%, 8%, or 9%, etc.

[0017] The percentage content of water can be 72%, 75%, 78%, 80%, 82%, 85%, 88%, 90%, 92%, or 94%, etc.

[0018] Preferably, the plant extract is licorice root extract, licorice root extract, and Chinese sweet potato root juice.

[0019] Preferably, the mass ratio of the extract of Glycyrrhiza uralensis root, the extract of Glycyrrhiza glabra root, and the juice of Dioscorea opposita root is (1-5):(0.05-1):(1-4).

[0020] The values ​​“1-5” can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5, 3.8, 4, 4.2, 4.5, or 4.8, etc.

[0021] "0.05-1" can be 0.06, 0.08, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.

[0022] "1-4" can be 1.2, 1.5, 1.8, 2, 2.2, 2.5, 2.8, 3, 3.2, 3.5 or 3.8, etc.

[0023] The plant composition of this invention contains *Glycyrrhiza uralensis* root extract and *Glycyrrhiza glabra* extract, which can directly and efficiently scavenge different types of reactive free radicals. *Glycyrrhiza uralensis* root extract contains glycyrrhizin A and glycyrrhizic acid, while *Glycyrrhiza glabra* extract is rich in glycyrrhizin, enhancing the activity of endogenous antioxidant enzymes and improving cellular antioxidant capacity through the Nrf2 / ARE pathway. Both extracts can also effectively reduce the expression of inflammatory factors induced by stimulation, inhibit pro-inflammatory cytokines (TNF-α, IL-6), block the NF-κB signaling pathway, and promote the recovery of skin barrier repair genes such as proteins. Furthermore, they can inhibit tyrosinase to reduce melanin formation, thus achieving a whitening effect. The abundant vitamin C and flavonoids in *Ipomoea aquatica* root juice can provide another layer of antioxidant support and may regenerate other antioxidants, forming a more comprehensive antioxidant network to protect skin cells from oxidative stress damage. Inulin in *Ipomoea aquatica* root juice belongs to the fructooligosaccharide class and is a natural moisturizing factor that can absorb water, form a moist film, reduce water loss, and exert a moisturizing effect. The combination of licorice root extract, licorice root extract, and Chinese sweet potato root juice can synergistically reduce skin inflammation and accelerate the repair of the physical and moisturizing barriers.

[0024] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0025] In a second aspect, the present invention provides a method for preparing the soothing and repairing composition as described in the first aspect. The preparation method includes heating water to 35-45°C (e.g., 36°C, 38°C, 40°C, 42°C, or 44°C, etc.), adding ceramide, phytosterol, superoxide dismutase, and plant extract, stirring evenly, and then adding cyclodextrin and mixing evenly to obtain the soothing and repairing composition.

[0026] Thirdly, the present invention provides the use of the soothing and repairing composition as described in the first aspect in the preparation of cosmetics.

[0027] Preferably, the cosmetic product includes any one of serum, lotion, cream, mask, or gel;

[0028] Preferably, the soothing and repairing composition has a percentage content of 5%-20% in the cosmetic, for example, it can be 6%, 8%, 10%, 12%, 14%, 16% or 18%, etc.

[0029] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0030] Fourthly, the present invention provides a soothing and repairing essence, the components of which include the soothing and repairing composition described in the first aspect, a thickener, a moisturizer, a skin conditioning agent, an ion regulator, and water.

[0031] Preferably, the components of the soothing and repairing essence, by mass fraction, include 5-15 parts of the soothing and repairing composition described in the first aspect, 5-10 parts of a moisturizer, 0.05-0.5 parts of a thickener, 0.5-5 parts of a skin conditioning agent, 0.01-0.05 parts of an ion regulator, and 70-90 parts of water.

[0032] The amount of the soothing and repairing composition added to the soothing and repairing essence of the present invention can be 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts or 14 parts, etc.

[0033] The amount of the humectant added can be 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, or 9.5 parts, etc.;

[0034] The amount of the thickener added can be 0.06 parts, 0.08 parts, 0.1 parts, 0.15 parts, 0.2 parts, 0.25 parts, 0.3 parts, 0.35 parts, 0.4 parts, or 0.45 parts, etc.;

[0035] The amount of the skin conditioning agent added can be 0.6 parts, 0.8 parts, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, or 4.5 parts, etc.;

[0036] The amount of the ion regulator added can be 0.02 parts, 0.03 parts, 0.04 parts, or 0.045 parts, etc.;

[0037] The amount of water added can be 72 parts, 75 parts, 78 parts, 80 parts, 82 parts, 85 parts, or 88 parts, etc.

[0038] Preferably, the moisturizer comprises any one or a combination of at least two of glycerin, butylene glycol, propylene glycol, 1,2-hexanediol, hydrolyzed sodium hyaluronate, ethylhexylglycerin, or acrylate copolymers.

[0039] Preferably, the thickener comprises xanthan gum and / or sodium hyaluronate.

[0040] Preferably, the ion regulator comprises sodium chloride.

[0041] Preferably, the skin conditioning agent comprises any one or a combination of at least two of the following: saccharide isomers, allantoin, α-glucan oligosaccharides, maltodextrin, glyceryl caprylate, polyglyceryl-10 oleate, phosphatidylcholine, Tremella fuciformis polysaccharide, Tremella fuciformis fruiting body extract, Centella asiatica extract, red algae extract, tea extract, Polygonum cuspidatum extract, Scutellaria baicalensis extract, Rosmarinus officinalis leaf extract, Chamomile extract, ginseng root extract, Lactobacillus fermentation filtrate, decapeptide-4, glycoprotein, acetyl hexapeptide-8, or hydrolyzed collagen.

[0042] Preferably, the skin conditioning agent is a combination of Tremella fuciformis polysaccharide, ginseng extract, acetyl hexapeptide-8, and hydrolyzed collagen.

[0043] Preferably, the mass ratio of the tremella polysaccharide, ginseng extract, acetyl hexapeptide-8 and hydrolyzed collagen is (1-4):(0.1-1):(1-2):(1-2).

[0044] The numbers “1-4” can be 1.1, 1.3, 1.5, 1.8, 2, 2.3, 2.5, 2.8, 3, 3.2, 3.5, 3.7, or 3.9, etc.

[0045] "0.1-1" can be 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, or 0.9, etc.;

[0046] The first "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8, or 1.9, etc.;

[0047] The second "1-2" can be 1.1, 1.2, 1.3, 1.4, 1.5, 1.6, 1.7, 1.8 or 1.9, etc.

[0048] As a preferred technical solution of the present invention, the tremella polysaccharide in the skin conditioning agent contains high molecular weight polysaccharide with a three-dimensional network water-retaining structure, forming a transparent, moist, and breathable film on the stratum corneum, which can effectively lock in water, improve the water content and barrier function of the stratum corneum, enhance skin elasticity and luster, and relieve skin sensitivity; the ginsenosides in the ginseng extract can scavenge free radicals, activate the activity of peroxidase in the body, inhibit inflammatory factors, reduce mitochondrial damage, inhibit MMP-1 expression, enhance cell vitality, resist photoaging, reduce collagen degradation, enhance skin firmness, and delay the formation of fine lines; acetyl hexapeptide-8 can inhibit nerve signals, competitively inhibit the formation of SNARE complex, reduce the release of acetylcholine from neurons, relax muscles, reduce the frequency of muscle contraction, and reduce micro-inflammation caused by facial expressions, thus achieving an anti-wrinkle effect; hydrolyzed collagen contains small molecule peptides and amino acids, which can provide amino acid raw materials for fibroblasts to synthesize collagen, stimulate fibroblast proliferation, and promote the synthesis of type I and type III collagen and elastic fibers. When used together, these four ingredients can further enhance the product's soothing and repairing effects and improve the skin's overall defense.

[0049] Preferably, the soothing and repairing essence also includes preservatives and / or pH adjusters.

[0050] Preferably, the preservative includes any one or a combination of at least two of 1,2-hexanediol, 1,2-pentanediol, or p-hydroxyacetophenone.

[0051] Preferably, the pH adjuster comprises disodium hydrogen phosphate and / or sodium dihydrogen phosphate.

[0052] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0053] Fifthly, the present invention provides a method for preparing the soothing and repairing essence as described in the third aspect, the method comprising:

[0054] (1) Mix and homogenize water, humectant and thickener to obtain mixture A;

[0055] (2) Mixture B is obtained by homogenizing the skin conditioning agent and the ion regulator;

[0056] (3) Mix mixture A and mixture B together and homogenize them. After cooling, add the soothing and repairing composition and stir evenly to obtain the soothing and repairing essence.

[0057] Steps (1) and (2) are not in any particular order.

[0058] Preferably, the temperature during the mixing and homogenization in step (1) is 80-85℃, for example, it can be 81℃, 82℃, 83℃ or 84℃.

[0059] Preferably, the mixing and homogenization in step (1) further includes the addition of a preservative;

[0060] Preferably, the temperature during the mixing and homogenization in step (2) is 80-85℃, for example, it can be 81℃, 82℃, 83℃ or 84℃.

[0061] Preferably, the cooling to 35-45°C in step (3) can be, for example, 36°C, 38°C, 40°C, 42°C, or 44°C;

[0062] Preferably, step (3) further includes adding a pH adjuster during stirring.

[0063] All the specific point values ​​within the above range can be selected, and will not be elaborated on here.

[0064] Compared with the prior art, the present invention has the following beneficial effects:

[0065] In the composition of this invention, ceramide, as a core component of intercellular lipids in the skin barrier, can directly replenish barrier lipids, maintain the integrity of the intercellular lipid bilayer structure of the stratum corneum, and reduce the penetration of external irritants. Phytosterols, with a structure similar to cholesterol, can penetrate into the stratum corneum and synergistically enhance lipid barrier stability with ceramides, while also exerting anti-inflammatory effects by inhibiting the release of inflammatory mediators. Superoxide dismutase (SOD), as an antioxidant enzyme, can specifically catalyze the conversion of superoxide anion free radicals into hydrogen peroxide and oxygen, reducing free radical-mediated inflammatory damage and barrier disruption, thus achieving synergistic anti-inflammatory and antioxidant effects. When used in combination with plant extracts, these three components can give the composition excellent repairing, soothing, anti-inflammatory, and antioxidant effects. In addition, the use of cyclodextrin in the composition of this invention can improve the stability and transdermal permeability of the active ingredients and further regulate the skin feel of the product. Attached Figure Description

[0066] Figure 1 Example 1 of the application of this invention is a schematic diagram showing the facial skin comparison of a volunteer before and after using the soothing and repairing essence;

[0067] Figure 2 Example 3 of the application of the present invention is a schematic diagram showing the facial skin comparison of a volunteer before and after using the soothing and repairing essence. Detailed Implementation

[0068] The technical solution of the present invention will be further illustrated below through specific embodiments. Those skilled in the art should understand that the embodiments described are merely illustrative of the present invention and should not be construed as limiting the invention in any way.

[0069] The weight parts mentioned in the following examples and comparative examples are based on the actual active ingredients contained in commercially available raw materials. These commercially available raw materials may also selectively contain any one or a combination of at least two of the following: solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants, or impurities within permissible limits.

[0070] The sources of some of the raw materials used in the embodiments or comparative examples of this invention are as follows:

[0071]

[0072] All other raw materials can be used as long as they are purchased from authorized distributors.

[0073] Example 1

[0074] This embodiment provides a soothing and repairing composition, which, by weight percentage, comprises 0.1% ceramide, 0.12% phytosterol, 6 ppm superoxide dismutase, 10% cyclodextrin, and 8% plant extract, with the remainder being water to bring the total to 100%.

[0075] The plant composition consists of Glycyrrhiza uralensis root extract, Glycyrrhiza glabra extract, and Ipomoea aquatica root juice in a mass ratio of 3:0.5:2.5.

[0076] The method for preparing the soothing and repairing composition is as follows:

[0077] After heating the water to 40°C, add ceramide, phytosterol, superoxide dismutase and plant extract, stir well, then add cyclodextrin and mix well to obtain the soothing and repairing composition.

[0078] Example 2

[0079] This embodiment provides a soothing and repairing composition, which, by weight percentage, comprises 0.15% ceramide, 0.05% phytosterol, 5 ppm superoxide dismutase, 8% cyclodextrin, and 10% plant extract, with the remainder made up to 100% by water.

[0080] The plant composition is composed of Glycyrrhiza uralensis root extract, Glycyrrhiza glabra extract and Ipomoea aquatica root juice in a mass ratio of 5:0.3:1.

[0081] The preparation method of the soothing and repairing composition is as described in Example 1.

[0082] Example 3

[0083] This embodiment provides a soothing and repairing composition, which, by weight percentage, comprises 0.05% ceramide, 0.15% phytosterol, 8 ppm superoxide dismutase, 15% cyclodextrin, and 6% plant extract, with the remainder being water to bring the total to 100%.

[0084] The plant composition consists of Glycyrrhiza uralensis root extract, Glycyrrhiza glabra extract, and Ipomoea batatas root juice in a mass ratio of 1:1:4.

[0085] The preparation method of the soothing and repairing composition is as described in Example 1.

[0086] Example 4

[0087] This embodiment provides a soothing and repairing composition. The only difference between this soothing and repairing composition and that of Example 1 is that the plant extracts are Glycyrrhiza inflata root extract and Glycyrrhiza glabra extract in a mass ratio of 6:1, and the amount of plant extracts remains unchanged. The other components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0088] Example 5

[0089] This embodiment provides a soothing and repairing composition. The only difference between this composition and that of Example 1 is that the plant extracts are licorice root extract and Japanese sweet potato root juice in a mass ratio of 6:5, and the amount of plant extracts remains unchanged. The other components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0090] Example 6

[0091] This embodiment provides a soothing and repairing composition. The only difference between this composition and that of Example 1 is that the plant extracts are licorice root extract and Chinese sweet potato root juice in a mass ratio of 1:5, and the amount of plant extracts remains unchanged. The other components and their amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0092] Comparative Example 1

[0093] This comparative example provides a soothing and repairing composition. The only difference between this soothing and repairing composition and Example 1 is that it does not include ceramides in its components. The reduction in ceramides is made up by phytosterols, superoxide dismutase and plant extracts in a mass percentage ratio. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0094] Comparative Example 2

[0095] This comparative example provides a soothing and repairing composition. The only difference between this soothing and repairing composition and Example 1 is that it does not include phytosterols in its components. The reduction in phytosterols is made up by ceramides, superoxide dismutase and plant extracts in a mass percentage ratio. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0096] Comparative Example 3

[0097] This comparative example provides a soothing and repairing composition. The only difference between this soothing and repairing composition and Example 1 is that it does not include superoxide dismutase in its components. The reduction in superoxide dismutase is made up by ceramide, phytosterol and plant extract in the mass percentage ratio. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0098] Comparative Example 4

[0099] This comparative example provides a soothing and repairing composition. The only difference between this soothing and repairing composition and Example 1 is that it does not include plant extracts in its components. The reduction in the amount of plant extracts is made up by ceramides, phytosterols and superoxide dismutase in a mass percentage ratio. The remaining components and amounts are the same as in Example 1, and the preparation method is the same as in Example 1.

[0100] Application Examples 1-3

[0101] The application examples provide three soothing and repairing serums, with the following formulas (all amounts are parts per serving):

[0102]

[0103] Its preparation method is as follows:

[0104] (1) Mix and homogenize water, humectant, preservative and thickener at 83°C to obtain mixture A;

[0105] (2) The skin conditioning agent and the ion regulator were heated to 83°C and then mixed and homogenized to obtain mixture B;

[0106] (3) Mix mixture A and mixture B together and homogenize. After cooling to 40°C, add the soothing and repairing composition, stir evenly, add pH adjuster to adjust pH to 6.5, and obtain the soothing and repairing essence.

[0107] Application Example 4-6

[0108] The application examples provide three soothing and repairing essences. The only difference between the formula and application example 1 is that the soothing and repairing composition of example 1 is replaced with an equal amount of the soothing and repairing composition of examples 4-6. The remaining components and dosages are the same as in application example 1, and the preparation method is the same as in application example 1.

[0109] Application Example 7

[0110] This application example provides a soothing and repairing essence. The only difference between its formula and that of Application Example 1 is that the skin conditioning agent does not include Tremella polysaccharide. The reduced amount is made up by ginseng extract, acetyl hexapeptide-8 and hydrolyzed collagen in proportion. The remaining components and dosages are the same as those in Application Example 1, and the preparation method is the same as that in Application Example 1.

[0111] Application Example 8

[0112] This application example provides a soothing and repairing essence. The only difference between its formula and that of Application Example 1 is that the skin conditioning agent does not include ginseng extract. The reduced amount is made up by tremella polysaccharide, acetyl hexapeptide-8 and hydrolyzed collagen in proportion. The remaining components and dosages are the same as those in Application Example 1, and the preparation method is the same as that in Application Example 1.

[0113] Application Example 9

[0114] This application example provides a soothing and repairing essence. The only difference between its formula and that of Application Example 1 is that the skin conditioning agent does not include acetyl hexapeptide-8. The reduced amount is made up by tremella polysaccharide, ginseng extract and hydrolyzed collagen in proportion. The remaining components and dosages are the same as those in Application Example 1, and the preparation method is the same as that in Application Example 1.

[0115] Application Example 10

[0116] This application example provides a soothing and repairing essence. The only difference between its formula and that of Application Example 1 is that the skin conditioning agent does not contain hydrolyzed collagen. The reduced amount is made up by Tremella fuciformis polysaccharide, ginseng extract and acetyl hexapeptide-8 in proportion to the amount of each ingredient. The remaining components and amounts are the same as in Application Example 1, and the preparation method is the same as in Application Example 1.

[0117] Compare and contrast examples 1-4

[0118] The comparative application examples provide four soothing and repairing essences. The only difference between the formula and application example 1 is that the soothing and repairing composition of example 1 is replaced with an equal amount of the soothing and repairing composition of comparative examples 1-4. The remaining components and dosages are the same as in application example 1, and the preparation method is the same as in application example 1.

[0119] Comparative Application Example 5

[0120] This comparative application example provides a soothing and repairing essence. The only difference between its formula and application example 1 is that it does not include the soothing and repairing composition of example 1 in its components. The reduction is made up by water. The remaining components and dosages are the same as in application example 1, and the preparation method is the same as in application example 1.

[0121] Test Example 1 Antioxidant Test

[0122] Test subjects: Compositions of Examples 1-6 and Comparative Examples 1-4.

[0123] Test method: Take 1 g of the product from Example 1, add 2 mL of anhydrous ethanol, dissolve completely, then add 2 mL of 60 μmol / L DPPH solution and mix well. Let stand for 30 min, zero the sample with anhydrous ethanol, and measure the absorbance at 517 nm as Ai; in the same way, take 2 mL of anhydrous ethanol solvent + 2 mL of DPPH solution, mix well and measure the absorbance as Ac; then take 1 g of the composition matrix, dissolve it, add 2 mL of anhydrous ethanol, then add 2 mL of anhydrous ethanol solvent, mix well and measure the absorbance as Aj.

[0124] Calculate its free radical scavenging rate using the following formula.

[0125] Clearance rate (%) = [1 - (Ai - Aj) / Ac] × 100%

[0126] Where Aj represents the contribution of the sample itself to the absorbance; Ai represents the absorbance of the sample after the DPPH treatment; and Ac represents the absorption of DPPH itself at the measurement wavelength.

[0127] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better its antioxidant capacity and the better its anti-aging effect. Therefore, the anti-aging effect can be judged by studying the ability of the composition to scavenge DPPH free radicals.

[0128] The test methods for Examples 2-6 and Comparative Examples 1-4 are the same as those described above.

[0129] The results are shown in Table 1 (each group of samples was measured in parallel 5 times, and the data are presented as average values).

[0130] Table 1

[0131]

[0132] As shown in Table 1, the soothing and repairing compositions of Examples 1-3 of the present invention can achieve a DPPH free radical scavenging rate of over 80%, indicating that the compositions have very good antioxidant activity, can effectively remove skin free radicals, and reduce oxidative stress-related inflammatory responses.

[0133] The samples in Examples 4-6 showed a decrease in DPPH free radical scavenging rate, indicating that the choice of plant composition has an impact on antioxidant performance, and that the three components of Glycyrrhiza uralensis root extract, Glycyrrhiza glabra root extract and Dioscorea opposita root juice work synergistically and are indispensable.

[0134] As can be seen from Comparative Examples 1-4, the ceramide, phytosterol, superoxide dismutase and plant extract in the composition of the present invention have a synergistic effect, and the absence of any one of them will reduce the antioxidant capacity to a certain extent.

[0135] Test Example 2: Hyaluronidase Inhibition Experiment

[0136] Hyaluronidase is a specific cleavage enzyme of hyaluronic acid and is often used to evaluate in vitro anti-allergic activity. Hyaluronic acid plays a major inhibitory role in human development and regulation processes (such as cell adhesion, organogenesis, wound healing, tumorigenesis, and angiogenesis). Inhibiting hyaluronidase activity can prevent sodium hyaluronate from being broken down and maintain normal physiological function. Therefore, the hyaluronidase inhibition rate is used as the anti-allergic standard, and the specific grading standards are shown in Table 2.

[0137] Table 2

[0138]

[0139] Test method: Transfer 0.1 mL of 0.25 mmol / L CaCl2 solution, 0.5 mL of 500 U / mL hyaluronidase solution, and an appropriate amount of acetate buffer (pH=5.6) into 20 Erlenmeyer flasks, gently shake to mix, and incubate at 37℃ for 20 min. Then, add 0.5 mL of deionized water and 0.5 mL of the sample from Example 1, incubate at 37℃ for 20 min, then add 0.5 mL of 0.5 mg / mL potassium hyaluronidase solution and an appropriate amount of acetate buffer (pH=5.6), incubate at 37℃ for 40 min, then let stand at room temperature for 10 min. Add 0.5 mL of deionized water, 1 drop of 5 mol / L NaOH solution, and 1 mL of acetylacetone solution, heat in a boiling water bath for 15 min, immediately cool with ice water for 5 min, let stand at room temperature for 10 min, then add 1 mL of P-DAB colorimetric reagent and an appropriate amount of anhydrous ethanol, and let stand at room temperature for 30 min. The color development was performed at 10 min, and then the absorbance of the sample at 530 nm was measured using a spectrophotometer. The inhibition rate of each group of samples against hyaluronidase was calculated, and a 5% dipotassium glycyrrhizate solution was used as a positive control. The calculation formula is shown in (1-1), and the calculation results are shown in Table 4.

[0140] (1-1)

[0141] Where: I represents the hyaluronidase inhibition rate;

[0142] A1 is the absorbance value of the solution of (hyaluronidase + sample + potassium hyaluronidase);

[0143] A2 is the absorbance value of the (acetic acid buffer + sample + acetate buffer) solution;

[0144] A3 is the absorbance value of the solution of (hyaluronidase + deionized water + potassium hyaluronidase);

[0145] A4 represents the absorbance value of the (acetic acid buffer + deionized water) solution.

[0146] The test methods for Examples 2-6 and Comparative Examples 1-4 are the same as those described above.

[0147] The results are shown in Table 3.

[0148] Table 3

[0149]

[0150] As shown in Table 3, the compositions prepared in Examples 1-3 all exhibited an inhibition rate of over 70% against hyaluronidase, indicating that the compositions provided by this invention have significant anti-allergic effects. Furthermore, the products from Comparative Examples 1-4 demonstrate that the ceramides, phytosterols, superoxide dismutase, and plant extracts described in this invention work synergistically to enhance anti-allergic effects. Compositions lacking any of these active ingredients will exhibit reduced anti-allergic capabilities.

[0151] Test Example 3: Skin Barrier Strengthening Experiment

[0152] One hundred and five female volunteers aged 20–40 years were randomly selected as the study subjects. They had no skin problems at the test sites within the past month and no history of systemic diseases. The volunteers were randomly divided into 15 groups of seven. Before the test, volunteers were asked to wipe their arms with water and sit quietly for 30 minutes in a constant temperature and humidity environment (temperature: 25±1℃, relative humidity: 40±5%), remaining relaxed. A 4 cm × 4 cm test area was marked on the inner side of the volunteer's arm, 5 cm from the base of the palm. Multiple areas could be marked on the same arm simultaneously (spaced 1 cm apart). The test product was randomly applied to the marked areas. For four consecutive weeks, the product (samples prepared in Examples 1–10 and Comparative Examples 1–5) was applied to the marked areas of the volunteers, at a dosage of 2.0 ± 0.1 μL / cm². 2 During the trial, participants were not allowed to apply any drugs or cosmetics unrelated to the experiment. Transdermal water loss was measured and recorded using a Tewameter™ 300 device before application, at week 1 of application, and at week 2 of application (TEWL0, TEWL1, TEWL2). The test results are shown in Table 4 (the results are the average of the results of 7 subjects).

[0153] Table 4

[0154]

[0155] As shown in Table 4, the soothing and repairing essence involved in this invention has excellent barrier repair ability, thereby enabling the skin to have excellent water-locking ability.

[0156] As can be seen from application examples 7-10, the choice of skin conditioning agent has a certain impact on the barrier repair ability of the product. Only when the four components of Tremella fuciformis polysaccharide, ginseng extract, acetyl hexapeptide-8 and hydrolyzed collagen are used in combination can the product's efficacy be more significant.

[0157] Test Example 4: Soothing Test

[0158] Test subjects: Soothing and repairing essences prepared using Examples 1-10 and comparison examples 1-5.

[0159] Test Method: The same group of volunteers as in Test Example 3, with the same grouping, were used. On the morning of the test, participants were not allowed to wear makeup or use skincare products. After cleansing their faces with water, they waited 30 minutes in a temperature- and humidity-controlled room. Then, 10% lactic acid was applied to one side of the nasolabial fold at room temperature. Five minutes after application, the samples from Application Examples 1-10 and Control Application Examples 1-5 were used. After 30 minutes, volunteers rated their experience using a 5-point scale: 4.5 or higher indicated excellent (very satisfied); 4-4.5 indicated good; 3-4 indicated acceptable; and below 3 indicated poor (unacceptable). The scores for each group were the average of 7 participants. The results are shown in Table 5.

[0160] Table 5

[0161]

[0162] As shown in Table 5, compared with Comparative Application Example 5, the products prepared in Application Examples 1-3 of the present invention can quickly regulate and soothe skin discomfort symptoms, have excellent immediate soothing effects, and volunteers had a good experience after using them, indicating that the composition provided by the present invention has a rapid soothing effect.

[0163] Test Example 5: Safety Evaluation Experiment

[0164] The specific method was as follows: Forty women aged 18-35 years with no history of allergies were selected. Each subject used samples from Examples 1-10. After cleaning the subject's arm, the patch applicator containing the sample was applied to the selected location on the arm using non-irritating adhesive tape. After application, the patch was gently pressed with the fingers to ensure even adhesion to the skin, and left on for 48 hours. During these 48 hours, the subject kept the patch area dry and avoided strenuous exercise, scratching, and prolonged sun exposure. After 48 hours, the applicator was removed and marked. After 30 minutes, once the indentation disappeared, the results were assessed under sufficient light.

[0165] The grading criteria for adverse skin reactions are shown in Table 6:

[0166] Table 6

[0167]

[0168] The results are shown in Table 7:

[0169] Table 7

[0170]

[0171] As shown in Table 7, no adverse skin reactions were observed in the volunteers after the spot tester was removed. This indicates that the raw materials used in the product of this invention are safe and non-irritating, have no adverse reactions on the human body, and are highly safe.

[0172] The applicant declares that the present invention is illustrated by the above embodiments to demonstrate a soothing and repairing composition, its preparation method, and its application. However, the present invention is not limited to the above embodiments, i.e., it does not mean that the present invention must rely on the above embodiments to be implemented. Those skilled in the art should understand that any improvements to the present invention, equivalent substitutions of the raw materials of the product, addition of auxiliary components, and selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0173] The preferred embodiments of the present invention have been described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various simple modifications can be made to the technical solution of the present invention, and these simple modifications all fall within the protection scope of the present invention.

[0174] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not describe the various possible combinations separately.

Claims

1. A soothing repair composition characterized in that, The soothing repair composition comprises ceramide, phytosterol, superoxide dismutase, cyclodextrin, plant extract and water. The plant extract comprises any one or a combination of at least two of Glycyrrhiza inflata root extract, Glycyrrhiza glabra extract or Inula belamcanda root juice.

2. The soothing repair composition of claim 1, wherein The soothing repair composition comprises ceramide 0.02%-0.15%, phytosterol 0.01%-0.15%, superoxide dismutase 0.4-10ppm, cyclodextrin 5%-15%, plant extract 1%-10% and water 70-95% by mass percentage.

3. The soothing repair composition of claim 1 or 2, wherein The plant extract is Glycyrrhiza inflata root extract, Glycyrrhiza glabra extract and Inula belamcanda root juice. Preferably, the mass ratio of the Glycyrrhiza inflata root extract, Glycyrrhiza glabra extract and Inula belamcanda root juice is (1-5):(0.05-1):(1-4).

4. A method of preparing a soothing repair composition according to any one of claims 1 to 3, characterized in that, The preparation method comprises heating water to 35-45℃, adding ceramide, phytosterol, superoxide dismutase and plant extract, stirring uniformly, then adding cyclodextrin and mixing uniformly to obtain the soothing repair composition.

5. Use of the soothing repair composition according to any one of claims 1-3 in the preparation of a cosmetic product.

6. The use according to claim 5, wherein the compound is ###0002### The cosmetic product comprises any one of essence, emulsion, cream, mask or jelly. Preferably, the percentage content of the soothing repair composition in the cosmetic product is 5%-20%.

7. A soothing repair serum, characterized in that, The components of the soothing repair essence comprise the soothing repair composition according to any one of claims 1-3, thickening agent, moisturizing agent, skin conditioning agent, ion regulator and water.

8. The soothing repair serum of claim 7, wherein, The components of the soothing repair essence comprise the soothing repair composition according to any one of claims 1-3 5-15 parts, moisturizing agent 5-10 parts, thickening agent 0.05-0.5 parts, skin conditioning agent 0.5-5 parts, ion regulator 0.01-0.05 parts and water 70-90 parts by mass fraction. Preferably, the moisturizing agent comprises any one or a combination of at least two of glycerin, butylene glycol, propylene glycol, 1,2-hexanediol, sodium hyaluronate, ethylhexylglycerin or acrylate copolymer. Preferably, the thickening agent comprises xanthan gum and / or sodium hyaluronate. Preferably, the ion regulator comprises sodium chloride.

9. The soothing repair serum according to claim 7 or 8, wherein The skin conditioning agent comprises any one or a combination of at least two of sugar isomer, allantoin, alpha-glucan oligosaccharide, maltodextrin, glyceryl caprylate, polyglyceryl-10 oleate, phosphatidylcholine, tremella fuciformis polysaccharide, tremella fruit body extract, gota kola extract, red algae extract, tea leaf extract, giant knotweed extract, radix scrophulariae extract, rosemary leaf extract, chamomile flower extract, ginseng root extract, lactobacillus ferment lysate, decapeptide-4, glycoprotein, acetyl hexapeptide-8 or hydrolyzed collagen. Preferably, the skin conditioning agent is a combination of tremella fuciformis polysaccharide, ginseng extract, acetyl hexapeptide-8 and hydrolyzed collagen. Preferably, the components of the soothing repair essence further comprise preservative and / or pH regulator. Preferably, the preservative comprises any one or a combination of at least two of 1,2-hexanediol, 1,2-pentanediol or p-acetylephedrine; Preferably, the pH regulator comprises disodium hydrogen phosphate and / or sodium dihydrogen phosphate.

10. A method of preparing a soothing repair serum as claimed in any one of claims 7-9, characterized in that, The preparation method comprises: (1) mixing homogeneously water, moisturizer and thickening agent to obtain mixture A; (2) mixing homogeneously skin conditioner and ion regulator to obtain mixture B; (3) mixing homogeneously mixture A and mixture B, adding soothing repair composition after cooling, and stirring homogeneously to obtain the soothing repair essence; Step (1) and step (2) are not in a specific order; Preferably, the temperature during mixing homogeneously in step (1) is 80-85℃; Preferably, the mixing homogeneously in step (1) further comprises adding preservative; Preferably, the temperature during mixing homogeneously in step (2) is 80-85℃; Preferably, the cooling in step (3) is to 35-45℃; Preferably, the stirring in step (3) further comprises adding pH regulator.

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