Composition for assisting in reducing blood fat and application thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a composition for assisting in reducing blood fat and application thereof. The composition for assisting in reducing blood fat is prepared from the following raw materials: 20 to 25 parts of radix puerariae, 8 to 12 parts of radix codonopsis, 8 to 12 parts of lotus leaves, 25 to 35 parts of fructus crataegi, 12 to 18 parts of semen cassiae, 10 to 15 parts of folium perillae, 10 to 15 parts of herba cichorii extract and 25 to 35 parts of fish oil. The composition is reasonable in compatibility and capable of reducing the blood fat level and improving the living quality of hyperlipidemia patients through multi-target accurate intervention, and the radix puerariae, the codonopsis pilosula, the purple perilla, the lotus leaves, the hawthorn fruits, the semen cassiae, the chicory extract and the fish oil in the formula have an obvious synergistic effect in the aspect of reducing blood fat. The composition is safe and effective, has no toxic or side effect compared with western medicines, and is convenient for large-scale popularization and application.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine technology, specifically relating to a composition for assisting in lowering blood lipids and its uses. Background Technology

[0002] Hyperlipidemia, also known as dyslipidemia, is involved in the development and progression of diseases in multiple systems throughout the body, such as atherosclerotic cardiovascular and cerebrovascular diseases, pancreatitis, and even critical illnesses like cancer. The main manifestations of hyperlipidemia include elevated levels of triglycerides (TG) and total cholesterol (TC).

[0003] With the improvement of living standards, the prevalence of hyperlipidemia is gradually increasing, and it is more common in obese and diabetic patients. The causal relationship between diet and the occurrence and treatment of diseases is an important research topic in traditional Chinese medicine theory, with a long history. Currently, commonly used drugs for treating hyperlipidemia in clinical practice include statins and fibrates, both of which have varying degrees of shortcomings or limitations.

[0004] Studies have shown that gut microbiota plays a crucial role in the dynamic stability of blood lipid levels through bile acid metabolism regulation, short-chain fatty acid production, and inflammatory factor release. Disruptions in gut microbiota homeostasis caused by a high-fat diet, such as decreased beneficial bacteria levels and increased endotoxin levels, are considered major underlying causes of lipid metabolism disorders. Traditional Chinese medicine, with its complex and diverse components and multiple targets, can reshape the gut microbiota ecosystem, repair barrier function, and regulate host lipid metabolism pathways, demonstrating unique potential in the intervention of hyperlipidemia.

[0005] Fish oil is a collective term for all oily substances found in fish, including body oil, liver oil, and brain oil. Unlike other animal fats rich in saturated fatty acids, fish oil is rich in n-3 polyunsaturated fatty acids (n-3 PUFAs), among which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) are essential nutrients for human growth and development. Studies have shown that fish oil has antioxidant properties, regulates blood lipids, enhances immunity, and helps prevent cardiovascular and cerebrovascular diseases. In addition, fish oil can promote brain cell growth and development, improve learning and memory abilities, and help prevent Alzheimer's disease.

[0006] Currently, fish oil is mainly processed into health products and medicines. Due to its fishy smell, its application in food is very limited, and the product forms are also relatively simple, mainly in capsule form.

[0007] Chen Yanfen's literature (Chen Yanfen, Li Xiaorong, Wang Ruxing, et al. Comparison of the effects of fish oil and atorvastatin on the levels of blood lipids and related inflammatory factors in patients with hyperlipidemia [J]. Chinese Journal of Tissue Engineering Research and Clinical Rehabilitation, 2007, (30): 5885-5888.) compared the effects of fish oil and atorvastatin on the levels of blood lipids and related inflammatory factors in patients with hyperlipidemia. The results showed that the combined use of fish oil and atorvastatin in the treatment of mixed hyperlipidemia can enhance its lipid-lowering effect and avoid the side effects such as increased myositis incidence when used in combination with fibrates.

[0008] Providing a safe and effective fish oil composition that can help lower blood lipids is an urgent problem to be solved. Summary of the Invention

[0009] In order to overcome the shortcomings and deficiencies of the prior art, the present invention provides a composition for assisting in lowering blood lipids and its application, which has good blood lipid lowering effect, no toxic side effects, and reasonable compatibility, and can effectively solve the problems existing in the prior art.

[0010] This invention provides a composition for assisting in lowering blood lipids, the composition comprising the following ingredients: kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, perilla, chicory extract, and fish oil.

[0011] Furthermore, the composition for assisting in lowering blood lipids includes the following ingredients: 20-25 parts of kudzu root, 8-12 parts of codonopsis root, 8-12 parts of lotus leaf, 25-35 parts of hawthorn, 12-18 parts of cassia seed, 10-15 parts of perilla, 10-15 parts of chicory extract, and 25-35 parts of fish oil.

[0012] Furthermore, the composition for assisting in lowering blood lipids comprises the following ingredients in parts by weight: 20 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 15 parts perilla, 10 parts chicory extract, and 30 parts fish oil.

[0013] Furthermore, the composition for assisting in lowering blood lipids comprises the following ingredients in parts by weight: 25 parts kudzu root, 12 parts codonopsis root, 8 parts lotus leaf, 25 parts hawthorn, 15 parts cassia seed, 10 parts perilla, 10 parts chicory extract, and 35 parts fish oil.

[0014] Furthermore, the composition for assisting in lowering blood lipids comprises the following ingredients in parts by weight: 20 parts kudzu root, 8 parts codonopsis root, 12 parts lotus leaf, 30 parts hawthorn, 18 parts cassia seed, 15 parts perilla, 15 parts chicory extract, and 30 parts fish oil.

[0015] The preparation method of the composition for assisting in lowering blood lipids in this invention is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, mix with water, decoct, filter, combine the filtrates, concentrate, and obtain concentrated liquid. Step S2: Preparation of chicory extract: Chicory was pulverized, and distilled water and papain were added. The pH was adjusted to 7.0, and the mixture was enzymatically hydrolyzed at 35℃-40℃ for 40-50 min. The enzyme was then inactivated by heating, and after cooling to room temperature, the pH was adjusted to 5.0-7.0. Trypsin was added, and the mixture was enzymatically hydrolyzed at 50℃-55℃ for 30-40 min. The enzyme was then inactivated by heating, and after cooling to room temperature, the mixture was centrifuged, the supernatant was collected, and the mixture was filtered to obtain the chicory extract. Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0016] Further, the preparation method of step S1 of the present invention is as follows: weigh kudzu root, codonopsis, lotus leaf, hawthorn, cassia seed and perilla according to the proportion of raw materials, add 8-12 times the mass of distilled water and decoct 2-3 times, each time for 30-50 minutes, filter, combine the filtrates, concentrate to obtain concentrated liquid.

[0017] Further, the preparation method of step S2 of the present invention is as follows: Chicory is crushed, 8-12 times the mass of distilled water is added, 0.5%-1.0% of the chicory mass of papain is added, the pH is adjusted to 7.0, and enzymatic hydrolysis is carried out at 35℃-40℃ for 40-50 min. The enzyme is inactivated by heating, and after cooling to room temperature, the pH is adjusted to 5.0-7.0, 0.5%-1.0% of the chicory mass of trypsin is added, and enzymatic hydrolysis is carried out at 50℃-55℃ for 30-40 min. The enzyme is inactivated by heating, and after cooling to room temperature, the mixture is centrifuged, the supernatant is collected, filtered, and the chicory extract is obtained.

[0018] Furthermore, the preparation method of the lipid-lowering composition of the present invention is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 10 times the weight of distilled water and decoct twice, each time for 35 minutes, filter, combine the filtrates, concentrate and obtain concentrated liquid. Step S2: Crush the chicory, add 10 times the weight of distilled water, add 0.5% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 40℃ for 50 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 6.0, add 1.0% of the chicory weight of trypsin, enzymatically hydrolyze at 55℃ for 30 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0019] The lipid-lowering composition of the present invention is prepared in the form of granules, capsules, tablets, pills, ointments or liquids.

[0020] Furthermore, the composition for assisting in lowering blood lipids is formulated as granules.

[0021] The present invention provides the use of the composition for assisting in lowering blood lipids in the preparation of adjuvant lipid-lowering drugs.

[0022] As can be seen from the above technical solution, compared with the prior art, the present invention provides a composition for assisting in lowering blood lipids, its preparation method and application, and has the following beneficial effects: Fish oil contains EPA, which can inhibit the action of bad lipoproteins, reduce their adhesion and deposition on the inner wall of blood vessels, and help high-density lipoprotein cholesterol remove excess fat from the blood vessel walls.

[0023] Polysaccharides, total saponins, polyphenols and alkaloids in traditional Chinese medicine have shown significant efficacy in treating hyperlipidemia, and long-term use has no obvious adverse reactions, which may become a new entry point for improving hyperlipidemia.

[0024] This invention provides a composition for assisting in lowering blood lipids. The composition is rationally formulated, with each ingredient synergistically enhancing its efficacy and function, resulting in a more comprehensive therapeutic effect on lowering blood lipids. The kudzu root, codonopsis root, perilla leaf, lotus leaf, hawthorn, cassia seed, chicory extract, and fish oil in the formula exhibit significant synergistic effects in treating and lowering blood lipids. Reducing the number of components significantly decreases the therapeutic activity of this composition for assisting in lowering blood lipids.

[0025] The composition of this invention for assisting in lowering blood lipids is safe and effective. Compared with Western medicine, it has no toxic side effects and is easy to promote and use on a large scale. Attached Figure Description

[0026] Figure 1 This section compares the serum TG levels in rats with those in the normal group. # P < 0.05 ## P < 0.01; compared with the model control group, * P < 0.05 ** P < 0.01; compared with group 1, & P < 0.05, compared with group 1, && P < 0.01.

[0027] Figure 2 This section compares the serum TC levels of rats with those of the normal group. # P < 0.05 ## P < 0.01; compared with the model control group, * P < 0.05 ** P < 0.01; compared with group 1,& P < 0.05, compared with group 1, && P < 0.01.

[0028] Figure 3 This study compares the serum HDL-C levels in rats with those in the normal group. # P < 0.05 ## P < 0.01; compared with the model control group, * P < 0.05 ** P < 0.01; compared with group 1, & P < 0.05, compared with group 1, && P < 0.01.

[0029] Figure 4 This study compares serum LDL-C levels in rats with those in the normal group. # P < 0.05 ## P < 0.01; compared with the model control group, * P < 0.05 ** P < 0.01; compared with group 1, & P < 0.05, compared with group 1, && P < 0.01. Detailed Implementation

[0030] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the specific implementation methods, structural features and effects of the present invention are described in detail below with reference to the accompanying drawings and embodiments.

[0031] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] All features disclosed in this specification, or all steps in all disclosed methods or processes, may be combined in any way, except for mutually exclusive features and / or steps.

[0033] Any feature disclosed in this specification (including any appended claims, abstract, and drawings) may be replaced by other equivalent or similar features, unless specifically stated otherwise. That is, unless specifically stated otherwise, each feature is merely one example of a series of equivalent or similar features.

[0034] Unless otherwise specified, the experimental methods used in the following examples are conventional methods, performed according to the techniques or conditions described in the literature in this field or according to the product instructions. Unless otherwise specified, the materials and reagents used in the following examples are commercially available.

[0035] Fish oil refers to lipids derived from higher animals, especially fish (such as cod, salmon), squid, and seals, particularly polyunsaturated fatty acids, including but not limited to Omega-3 unsaturated fatty acids, DHA, EPA, DPA, ALA, nisinic acid, stearidonic acid, eicosatetraenoic acid, or combinations thereof.

[0036] It is worth noting that the fish oil used in this invention is preferably provided by Xi'an Chaobang Biotechnology Co., Ltd., and is food grade.

[0037] Example 1 A composition for assisting in lowering blood lipids includes the following ingredients: 20 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 15 parts perilla, 10 parts chicory extract, and 30 parts fish oil.

[0038] The preparation method is as follows: Step S1: Weigh kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 10 times the mass of distilled water and decoct twice, each time for 35 minutes, filter, combine the filtrates, concentrate, and obtain concentrated liquid. Step S2: Crush the chicory, add 10 times the weight of distilled water, add 0.5% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 40℃ for 50 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 6.0, add 1.0% of the chicory weight of trypsin, enzymatically hydrolyze at 55℃ for 30 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0039] Example 2 A composition for assisting in lowering blood lipids includes the following ingredients: 25 parts of kudzu root, 12 parts of codonopsis root, 8 parts of lotus leaf, 25 parts of hawthorn, 15 parts of cassia seed, 10 parts of perilla, 10 parts of chicory extract, and 35 parts of fish oil.

[0040] The preparation method is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 8 times the mass of distilled water and decoct twice, each time for 30 minutes, filter, combine the filtrates, concentrate to obtain concentrated liquid; Step S2: Crush the chicory, add 8 times the weight of distilled water, add 1.0% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 35℃ for 40 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 7.0, add 0.5% of the chicory weight of trypsin, enzymatically hydrolyze at 50℃ for 40 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0041] Example 3 A composition for assisting in lowering blood lipids includes the following ingredients: 20 parts of kudzu root, 8 parts of codonopsis root, 12 parts of lotus leaf, 30 parts of hawthorn, 18 parts of cassia seed, 15 parts of perilla, 15 parts of chicory extract, and 30 parts of fish oil.

[0042] The preparation method is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 12 times the mass of distilled water and decoct 3 times, each time for 50 minutes, filter, combine the filtrates, concentrate and obtain concentrated liquid. Step S2: Crush the chicory, add 12 times the weight of distilled water, add 1.0% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 40℃ for 40 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 5.0, add 0.5% of the chicory weight of trypsin, enzymatically hydrolyze at 55℃ for 30 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0043] Example 4 A composition for assisting in lowering blood lipids includes the following ingredients: 25 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 35 parts hawthorn, 15 parts cassia seed, 15 parts perilla, 10 parts chicory extract, and 25 parts fish oil.

[0044] The preparation method is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 8 times the mass of distilled water and decoct 3 times, each time for 40 minutes, filter, combine the filtrates, concentrate and obtain concentrated liquid. Step S2: Crush the chicory, add 10 times the weight of distilled water, add 1.0% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 35℃ for 40 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 7.0, add 1.0% of the chicory weight of trypsin, enzymatically hydrolyze at 50℃ for 35 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0045] Example 5 A composition for assisting in lowering blood lipids includes the following ingredients: 20 parts kudzu root, 8 parts codonopsis root, 12 parts lotus leaf, 30 parts hawthorn, 12 parts cassia seed, 10 parts perilla, 15 parts chicory extract, and 35 parts fish oil.

[0046] The preparation method is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 12 times the mass of distilled water and decoct twice, each time for 50 minutes, filter, combine the filtrates, concentrate and obtain concentrated liquid. Step S2: Crush the chicory, add 8 times the weight of distilled water, add 1.0% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 40℃ for 40 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 6.0, add 1.0% of the chicory weight of trypsin, enzymatically hydrolyze at 55℃ for 35 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0047] Example 6 A composition for assisting in lowering blood lipids includes the following ingredients: 25 parts kudzu root, 12 parts codonopsis root, 8 parts lotus leaf, 30 parts hawthorn, 12 parts cassia seed, 15 parts perilla, 15 parts chicory extract, and 25 parts fish oil.

[0048] The preparation method is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 12 times the mass of distilled water and decoct twice, each time for 50 minutes, filter, combine the filtrates, concentrate and obtain concentrated liquid. Step S2: Crush the chicory, add 10 times the weight of distilled water, add 0.5% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 40℃ for 50 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 6.0, add 1.0% of the chicory weight of trypsin, enzymatically hydrolyze at 55℃ for 30 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0049] Example 7 A composition for assisting in lowering blood lipids includes the following ingredients: 25 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 25 parts hawthorn, 15 parts cassia seed, 25 parts perilla, 10 parts chicory extract, and 30 parts fish oil.

[0050] The preparation method is as follows: Step S1: Weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 10 times the weight of distilled water and decoct twice, each time for 35 minutes, filter, combine the filtrates, concentrate and obtain concentrated liquid. Step S2: Crush the chicory, add 12 times the weight of distilled water, add 1.0% of the chicory weight of papain, adjust the pH to 7.0, enzymatically hydrolyze at 40℃ for 40 min, heat to inactivate the enzyme, cool to room temperature, adjust the pH to 5.0, add 0.5% of the chicory weight of trypsin, enzymatically hydrolyze at 55℃ for 30 min, heat to inactivate the enzyme, cool to room temperature, centrifuge, collect the supernatant, filter, and obtain the chicory extract; Step S3: Mix the concentrated liquid obtained in step S1, chicory extract and fish oil, concentrate to obtain an extract, and dry to obtain the composition of the present invention for assisting in lowering blood lipids.

[0051] Comparative Example 1 Codonopsis pilosula 10 parts, lotus leaf 10 parts, hawthorn 30 parts, cassia seed 15 parts, perilla 15 parts, chicory extract 10 parts, fish oil 30 parts.

[0052] Compared with Example 1, the difference is that it does not contain kudzu root, while the other components and their amounts remain the same.

[0053] The preparation method is the same as in Example 1.

[0054] Comparative Example 2 30 parts kudzu root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 15 parts perilla, 10 parts chicory extract, and 30 parts fish oil.

[0055] Compared with Example 1, the difference is that it does not contain Codonopsis pilosula, while the other components and dosages remain the same.

[0056] The preparation method is the same as in Example 1.

[0057] Comparative Example 3 20 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 10 parts chicory extract, and 30 parts fish oil.

[0058] Compared with Example 1, the difference is that it does not contain perilla, while the other components and their amounts remain the same.

[0059] The preparation method is the same as in Example 1.

[0060] Comparative Example 4 20 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 15 parts perilla, and 10 parts chicory extract.

[0061] Compared with Example 1, the difference is that it does not contain fish oil, while the other components and amounts remain the same.

[0062] The preparation method is the same as in Example 1.

[0063] Comparative Example 5 20 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 15 parts perilla, and 30 parts fish oil.

[0064] Compared with Example 1, the difference is that it does not contain chicory extract, while the other components and amounts remain the same.

[0065] The preparation method is as follows: Step S1, weigh out kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the raw material ratio, add 10 times the weight of distilled water and decoct twice, each time for 35 minutes, filter, combine the filtrates, concentrate to obtain concentrated liquid. Step S2: Mix the concentrated liquid obtained in step S1 with fish oil, concentrate to obtain an extract, dry to obtain a composition.

[0066] Comparative Example 6 20 parts kudzu root, 10 parts codonopsis root, 10 parts lotus leaf, 30 parts hawthorn, 15 parts cassia seed, 15 parts perilla, 10 parts chicory water extract, and 30 parts fish oil.

[0067] Compared with Example 1, the difference is that the chicory extract is replaced with chicory water extract, while the other components and amounts remain unchanged.

[0068] The preparation method is as follows: Step S1: Weigh kudzu root, codonopsis root, lotus leaf, hawthorn, cassia seed, and perilla according to the proportion of raw materials, add 10 times the mass of distilled water and decoct twice, each time for 35 minutes, filter, combine the filtrates, concentrate, and obtain concentrated liquid. Step S2: Crush the chicory, add 10 times its weight of distilled water and decoct twice, 35 minutes each time. Filter, combine the filtrates, concentrate to obtain concentrated liquid, and obtain chicory water extract. Step S3: Mix the concentrated liquid obtained in step S1, the chicory water extract and the fish oil, concentrate to obtain an extract, dry to obtain a composition.

[0069] Experimental study of the lipid-lowering composition of the present invention on hyperlipidemic rats. (1) Establishment of a rat model of hyperlipidemia Seventy healthy SPF-grade SD rats were randomly divided into a control group and a model group. The control group rats were fed a normal diet during the experiment. The model group rats were fed a high-fat diet to establish a hyperlipidemia animal model. After one week of acclimatization, the rats were fed a high-fat diet containing 83.3% normal diet, 0.2% methylpyrimidine sulfide, 0.5% sodium cholate, 1% cholesterol, 10% lard, and 5% sucrose. They had free access to water and were fed this diet continuously for four weeks.

[0070] Four weeks later, all rats were fasted, blood was collected from the external jugular vein, serum was separated, and serum triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDLC) were measured using a fully automated biochemical analyzer.

[0071] (2) Experimental grouping and drug administration In addition to the normal group (physiological saline), 60 rats that successfully developed the model were selected based on their (TG+TC) values ​​and divided into 10 groups of 6 rats each. The grouping and gavage dosage are as follows: Normal group (physiological saline), model control group (physiological saline), groups 1-3 (corresponding to the lipid-lowering compositions of Examples 1, 2, and 3 above, respectively), and groups 4-9 (corresponding to Comparative Examples 1-6 above, respectively).

[0072] The gavage dose for groups 1-9 was 2.1 g / kg. The normal group and the model control group were given the same volume of physiological saline. The rats in each group were gavaged continuously for 28 days. During the experiment, except for the control group, the rats in the other groups continued to be fed a high-fat diet.

[0073] After four weeks of continuous gavage, blood was collected from the abdominal aorta and centrifuged to obtain serum for later use. The liver was removed and fixed in 10% formaldehyde solution. The arteries were removed and placed in an embedding box and stored at -40°C.

[0074] (3) Observation indicators and detection methods 1) Body weight measurement Weigh yourself once a week and calculate the dosage based on the latest weight.

[0075] 2) Blood lipid testing The levels of serum TG, TC, HDL-C, and LDL-C in rats after modeling and drug administration were detected by a fully automated biochemical analyzer.

[0076] Statistical methods The collected data were summarized using Excel, and the measurement data were expressed as mean ± standard deviation. The results were presented using GraphPad Prism 7.0 software for plotting and statistical analysis. Pairwise comparisons were performed using t-tests, and between-group comparisons were performed using one-way ANOVA. A p-value < 0.05 was considered statistically significant.

[0077] (4) Experimental results 1) Effects on rat body weight Note: Compared with the normal group. # P < 0.05 ## P < 0.01; Compared with the model control group, * P < 0.05 ** P < 0.01; Compared with group 1, & P < 0.05.

[0078] The results are shown in Table 1.

[0079] Compared with the normal group, the rats in the model control group had significantly higher body weight, indicating that the model was successfully established.

[0080] Compared with the model control group, the rats in each group of the examples showed a significant or highly significant reduction in body weight, indicating that each group of the examples could reduce body weight. Groups 1-3 of the present invention, especially Group 1, showed better results.

[0081] Compared with Group 1 (Example 1), there were statistically significant differences in the body weight of rats in Groups 4-9, indicating that the lipid-lowering composition of the present invention has a better effect on reducing body weight.

[0082] 2) Effects on blood lipid levels in rats Compared with the normal group, the serum TG, TC and LDL-C levels of rats in the model control group were increased, while the HDL-C level was decreased.

[0083] Compared with the model control group, the serum TG, TC, and LDL-C levels of rats in each group of the examples and the comparative groups were decreased, while the HDL-C level was increased, showing significant or highly significant differences, indicating that each group could improve blood lipid levels. Groups 1-3 of this invention, especially Group 1, showed better results.

[0084] Compared with Group 1 (Example 1), the levels of TG, TC, LDL-C, and HDL-C in Groups 4-9 showed statistically significant differences, indicating that the lipid-lowering composition of the present invention has a better effect on improving blood lipid levels. Results are shown in […]. Figures 1-4 .

[0085] In this study, rats were treated with a high-fat diet. After being given the lipid-lowering composition of the present invention, the serum TG, TC, and LDL-C levels of the rats were significantly reduced, while the HDL-C level was increased. This suggests that the lipid-lowering composition of the present invention has a significant effect on lipid metabolism in hyperlipidemic rats.

[0086] In summary, the embodiments of the present invention provide a composition for assisting in lowering blood lipids. The components work synergistically to reduce rat body weight, significantly reduce serum TG, TC, and LDL-C levels, and increase HDL-C levels, thereby improving blood lipid levels.

[0087] Compared with Example 1 of the present invention, Comparative Example 1, which does not contain kudzu root, has a significantly weakened lipid-lowering effect. Comparative Example 2, which does not contain codonopsis root, has a significantly weakened lipid-lowering effect compared with Example 1 of the present invention. Comparative Example 3, which does not contain perilla leaf, has a significantly weakened lipid-lowering effect compared with Example 1 of the present invention. Comparative Example 4, which does not contain fish oil, has a significantly weakened lipid-lowering effect compared with Example 1 of the present invention. Comparative Example 5, which does not contain chicory extract, has a significantly weakened lipid-lowering effect compared with Example 1 of the present invention. Comparative Example 6, which replaces chicory extract with chicory water extract, has a significantly weakened lipid-lowering effect compared with Example 1 of the present invention. The present invention provides a composition for assisting in lowering blood lipids, with a reasonable formulation and synergistic effect of each ingredient, resulting in a more comprehensive efficacy and function, and a positive therapeutic effect on lowering blood lipids. Kudzu root, codonopsis root, perilla leaf, lotus leaf, hawthorn, cassia seed, chicory extract, and fish oil in the formulation have a significant synergistic effect in treating and lowering blood lipids. Reducing the number of components significantly reduces the therapeutic activity of this composition for assisting in lowering blood lipids.

[0088] The above description, in conjunction with specific preferred embodiments, provides a further detailed explanation of the present invention. It should not be construed that the specific implementation of the present invention is limited to these descriptions. For those skilled in the art, various simple deductions or substitutions can be made without departing from the concept of the present invention, and all such modifications and substitutions should be considered within the scope of protection of the present invention.

Claims

1. A composition for assisting in lowering blood lipids, characterized by comprising: The auxiliary hypolipidemic composition comprises the following raw materials: 20-25 parts of pueraria, 8-12 parts of radix codonopsis, 8-12 parts of lotus leaf, 25-35 parts of hawthorn, 12-18 parts of cassia seed, 10-15 parts of perilla, 10-15 parts of chic extract, and 25-35 parts of fish oil.

2. The composition for assisting hypolipidemic action according to claim 1, wherein The auxiliary hypolipidemic composition comprises the following raw materials: 20 parts of pueraria, 10 parts of radix codonopsis, 10 parts of lotus leaf, 30 parts of hawthorn, 15 parts of cassia seed, 15 parts of perilla, 10 parts of chic extract, and 30 parts of fish oil.

3. The composition for assisting hypolipidemic action according to claim 1, wherein The auxiliary hypolipidemic composition comprises the following raw materials: 25 parts of pueraria, 12 parts of radix codonopsis, 8 parts of lotus leaf, 25 parts of hawthorn, 15 parts of cassia seed, 10 parts of perilla, 10 parts of chic extract, and 35 parts of fish oil.

4. The composition for assisting hypolipidemic action according to claim 1, wherein The auxiliary hypolipidemic composition comprises the following raw materials: 20 parts of pueraria, 8 parts of radix codonopsis, 12 parts of lotus leaf, 30 parts of hawthorn, 18 parts of cassia seed, 15 parts of perilla, 15 parts of chic extract, and 30 parts of fish oil.

5. The composition for assisting hypolipidemic action according to any one of claims 1 to 4, characterized by, The preparation method of the auxiliary hypolipidemic composition is as follows: Step S1, the pueraria, radix codonopsis, lotus leaf, hawthorn, cassia seed, and perilla are weighed according to the proportion of raw materials, mixed with water, decocted, filtered, the filtrates are combined, concentrated, and the concentrated liquid is obtained. Step S2, the chic extract is prepared: The chic is crushed, distilled water and papain are added, the pH is adjusted to 7.0, and the chic is enzymolyzed at 35-40°C for 40-50 min, the temperature is raised to inactivate the enzyme, and then the temperature is lowered to room temperature, the pH is adjusted to 5.0-7.0, trypsin is added, and the chic is enzymolyzed at 50-55°C for 30-40 min, the temperature is raised to inactivate the enzyme, and then the temperature is lowered to room temperature, centrifugation is performed, the supernatant is collected, filtered, and the chic extract is obtained. Step S3, the concentrated liquid obtained in step S1, the chic extract, and fish oil are mixed, concentrated to obtain an extract, dried, and the auxiliary hypolipidemic composition of the application is obtained.

6. The composition for assisting hypolipidemic action according to any one of claims 1 to 4, wherein Step S1 is as follows: the pueraria, radix codonopsis, lotus leaf, hawthorn, cassia seed, and perilla are weighed according to the proportion of raw materials, 8-12 times the mass of distilled water is added, decoction is performed for 2-3 times, each time for 30-50 min, filtration is performed, the filtrates are combined, concentrated, and the concentrated liquid is obtained.

7. The composition for assisting hypolipidemic action according to any one of claims 1 to 4, wherein The preparation method of the chic extract comprises the following steps: the chic is crushed, 8-12 times the mass of distilled water is added to the chic, 0.5-1.0% of the mass of the chic of papain is added, the pH is adjusted to 7.0, and the chic is enzymolyzed at 35-40°C for 40-50 min, the temperature is raised to inactivate the enzyme, and then the temperature is lowered to room temperature, the pH is adjusted to 5.0-7.0, 0.5-1.0% of the mass of the chic of trypsin is added, the chic is enzymolyzed at 50-55°C for 30-40 min, the temperature is raised to inactivate the enzyme, and then the temperature is lowered to room temperature, centrifugation is performed, the supernatant is collected, filtered, and the chic extract is obtained.

8. The composition for assisting hypolipidemic action according to any one of claims 1 to 4, wherein The preparation of the auxiliary hypolipidemic composition is granules, capsules, tablets, pills, pastes, or liquid agents.

9. The composition for assisting hypolipidemic action according to any one of claims 1 to 4, wherein The preparation of the auxiliary hypolipidemic composition is granules.

10. The use of the auxiliary hypolipidemic composition in any one of claims 1-4 in the preparation of a hypolipidemic drug.

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