Intelligent intraperitoneal injection auxiliary fixing device for laboratory mouse

Abstract

The invention relates to the technical field of laboratory devices, in particular to an intelligent intraperitoneal injection auxiliary fixing device for laboratory mice, which comprises a basic bearing module, and a mouse fixing module, a pressure monitoring module, a control module, an indication module and a power supply module which are mounted on the basic bearing module, wherein the mouse fixing module is used for fixing a mouse, the mouse fixing module comprises a lower arc-shaped latticed clamping plate, an upper arc-shaped clamping plate, a connecting piece and a hook lock, the lower arc-shaped latticed clamping plate and the upper arc-shaped clamping plate are connected through the connecting piece, and the upper arc-shaped clamping plate and the lower arc-shaped latticed clamping plate are jointly matched to clamp the mouse; the abdomen of the mouse faces the lower arc-shaped latticed clamping plate, the hook lock is used for fixing the upper arc-shaped clamping plate and the lower arc-shaped latticed clamping plate, the pressure monitoring module can monitor the stress condition of the mouse in real time, compressive stress or suffocation of the mouse is avoided, and animal welfare is improved.

Description

Technical Field

[0001] This invention relates to the field of laboratory equipment technology, and in particular to an intelligent intraperitoneal injection-assisted fixation device for laboratory mice. Background Technology

[0002] In biomedical research, laboratory mice are a good research model. In the construction of mouse models, intraperitoneal injection is a commonly used method, and the main methods and devices used are as follows:

[0003] 1. Hand gripping method: This method relies on the operator's experience. For those who are not skilled in the operation, it is very easy for mice to struggle, suffocate, or even scratch the experimenter. At the same time, the instability of the operation will affect the accuracy and repeatability of the injection, which does not conform to the "3R principle" of animal experiments, namely, reduce, replace, and optimize.

[0004] 2. Simple fixation devices of fixed size: such as plastic tubes, insert-type devices, etc. These devices have poor versatility, cannot adapt to mice of different sizes, and are prone to causing pressure stress or suffocation in animals. Furthermore, their structure often obstructs the injection field of view, is complex to operate, and lacks quantitative monitoring and intelligent feedback of the operation process. They cannot monitor whether the pressure during mouse fixation is excessive, cannot assist in judging the needle insertion position and angle, and cannot automatically record experimental data. This results in low standardization and data traceability, significant human error variations, affecting the reproducibility and accuracy of the experiment, leading to low reliability of experimental data.

[0005] To this end, an intelligent intraperitoneal injection auxiliary fixation device for laboratory mice is proposed to improve the standardization, visualization and datafication, reproducibility and animal welfare of intraperitoneal injection operations in mice. Summary of the Invention

[0006] The purpose of this invention is to provide an intelligent intraperitoneal injection-assisted fixation device for laboratory mice, thereby solving the problems mentioned in the background art. The specific technical solution is as follows:

[0007] To achieve the above and other related objectives, the present invention provides an intelligent intraperitoneal injection-assisted fixation device for laboratory mice, comprising a basic support module, and a mouse fixation module, a pressure monitoring module, a control module, an indicator module, and a power supply module mounted on the basic support module; wherein,

[0008] The mouse fixation module is used to fix mice. The mouse fixation module includes a lower arc-shaped mesh clamp, an upper arc-shaped clamp, a connector, and a hook lock. The lower arc-shaped mesh clamp and the upper arc-shaped clamp are connected by the connector. The upper arc-shaped clamp and the lower arc-shaped mesh clamp work together to clamp the mouse with the mouse's abdomen facing the lower arc-shaped mesh clamp. The hook lock is used to fix the upper arc-shaped clamp and the lower arc-shaped mesh clamp.

[0009] The pressure monitoring module is located on the lower side of the upper arc-shaped clamp and is used to detect the pressure applied by the upper arc-shaped clamp to the mouse. The pressure monitoring module is electrically connected to the control module. The control module has a pressure judgment system running inside. The pressure judgment system is set to read the pressure signal from the pressure monitoring module, determine whether the pressure is within the threshold range based on the pressure signal, and control the indicator module to work. The power supply module is used to supply power to the pressure monitoring module, control module, and indicator module.

[0010] Preferably, both the lower arc-shaped mesh clamp and the upper arc-shaped clamp adopt an arc-shaped steel wire mesh structure.

[0011] Preferably, the upper arc-shaped clamp also includes an arched portion, which is made of a flexible material.

[0012] Preferably, the pressure monitoring module uses a pressure sensing diaphragm, which is positioned on the lower side of the arched portion.

[0013] Preferably, the basic support module is also equipped with an electric winding mechanism, on which a rope is provided. The rope is connected to a hook lock. The electric winding mechanism is electrically connected to the control module and is controlled by the control module. When fixing the upper arc-shaped clamp and the lower arc-shaped mesh clamp, the rope is wrapped around the lower arc-shaped mesh clamp and the hook lock is hung on the upper arc-shaped clamp. When the pressure signal exceeds the threshold, the control module controls the electric winding mechanism to release the rope, thereby reducing the pressure on the mouse.

[0014] Preferably, a camera module is also provided on the basic support module. The camera module is used to acquire images of the mouse's abdomen and the syringe. The camera module is connected to the control module, which runs an injection navigation system.

[0015] Preferably, the injection navigation system is configured as follows: using the arc-shaped grid clamp as a reference, the system calculates the length of the mouse's trunk based on the acquired images of the mouse's abdomen, plans the intraperitoneal injection area and the needle insertion angle, calculates the injection site and needle insertion angle of the syringe based on the syringe image, and determines whether the syringe injection site falls within the intraperitoneal injection area and whether the needle insertion angle calculated based on the syringe image falls within the planned needle insertion angle range. Depending on whether all fall within the range, the indicator module issues different alerts: if all fall within the range, the indicator module issues a green alert; if only the syringe injection site falls within the intraperitoneal injection area, the indicator module issues a flashing green alert; if only the needle insertion angle calculated based on the syringe image falls within the planned needle insertion angle range, a flashing red alert is issued; if none fall within the range, the indicator module issues a red alert.

[0016] Preferably, the control module is wirelessly connected to a display terminal, which can be a web page port or an APP software port. The display terminal displays the image captured by the camera module. The injection navigation system is also set to mark the injection area and the guide frame line of the needle insertion angle range in real time on the display screen of the display terminal.

[0017] The present invention provides an intelligent intraperitoneal injection-assisted fixation device for laboratory mice, which has the following beneficial effects:

[0018] 1. By setting up a pressure monitoring module, a control module, and an indicator module, the pressure monitoring module can monitor the stress on the mice in real time, avoiding pressure stress or suffocation and improving animal welfare;

[0019] 2. By setting up an electric winding mechanism and rope, when the pressure signal exceeds the threshold, the control module controls the electric winding mechanism to release the rope, thereby reducing the pressure on the mouse and keeping the mouse under appropriate pressure without the need for manual release and adjustment.

[0020] 3. By setting up a camera module and an injection navigation system, the length of the mouse trunk is calculated based on the acquired images of the mouse abdomen, the intraperitoneal injection area and the needle insertion angle are planned, the injection site and needle insertion angle of the syringe are calculated based on the syringe image, and it is determined whether the syringe injection site falls within the intraperitoneal injection area and whether the needle insertion angle calculated based on the syringe image falls within the planned needle insertion angle range. This achieves standardization, visualization, datafication, and repeatability of intraperitoneal injection operation in mice. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0022] Figure 1 This is a three-dimensional structural schematic diagram of the present invention;

[0023] Figure 2 This is a three-dimensional structural schematic diagram of the present invention from another perspective. Detailed Implementation

[0024] The following detailed description, in conjunction with the accompanying drawings and specific embodiments, provides a more detailed explanation of the intelligent intraperitoneal injection auxiliary fixation device for laboratory mice proposed in this invention. The advantages and features of the invention will become clearer from the following description. It should be noted that the accompanying drawings are all in a very simplified form and use non-precise proportions, and are only used to facilitate and clarify the illustration of the embodiments of the invention.

[0025] In the description of this invention, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0026] It should be noted that the illustrations provided in the following embodiments are only schematic representations of the basic concept of the present invention. Therefore, the illustrations only show the components related to the present invention and are not drawn according to the actual number, shape and size of the components in the actual implementation. In the actual implementation, the state, quantity and proportion of each component can be arbitrarily changed, and the layout of the components may also be more complex.

[0027] like Figure 1-2 As shown, an intelligent intraperitoneal injection-assisted fixation device for laboratory mice includes a basic support module 1, a pressure monitoring module 3, and a mouse fixation module 2, a control module 4, an indicator module 5, and a power supply module 6 mounted on the basic support module 1; wherein,

[0028] The mouse fixation module 2 is used to fix the mouse. The mouse fixation module 2 includes a lower arc-shaped mesh clamp 21, an upper arc-shaped clamp 22, a connector 23, and a hook lock 24. The lower arc-shaped mesh clamp 21 and the upper arc-shaped clamp 22 are connected by the connector 23. The upper arc-shaped clamp 22 and the lower arc-shaped mesh clamp 21 work together to clamp the mouse with the mouse's abdomen facing the lower arc-shaped mesh clamp 21. The hook lock 24 is used to fix the upper arc-shaped clamp 22 and the lower arc-shaped mesh clamp 21. In some embodiments, both the lower arc-shaped mesh clamp 21 and the upper arc-shaped clamp 22 adopt an arc-shaped steel wire mesh structure. In some embodiments, the upper arc-shaped clamp 22 also includes an arched part 25, which is made of flexible material.

[0029] The pressure monitoring module 3 is located on the lower side of the upper arc-shaped clamp 22 and is used to detect the pressure applied by the upper arc-shaped clamp 22 to the mouse. The pressure monitoring module 3 is electrically connected to the control module 4. The control module 4 has a pressure judgment system running inside. The pressure judgment system is set to read the pressure signal of the pressure monitoring module 3, and determine whether the pressure is within the threshold range based on the pressure signal. It also controls the indicator module 5 to work to display the reminder signal corresponding to the pressure signal. The power module 6 is used to supply power to the pressure monitoring module 3, the control module 4, and the indicator module 5. In some embodiments, the pressure monitoring module 3 uses a pressure sensing film, which is located on the lower side of the arch 25. The control module 4 uses an ESP32 controller, and the indicator module 5 is an LED light. When the pressure signal exceeds the threshold, a red reminder is issued; when the pressure signal meets the threshold, a green reminder is issued.

[0030] When using, grasp the mouse by the tail and place the mouse between the lower arc-shaped mesh clamp 21 and the upper arc-shaped clamp 22, with the mouse's abdomen facing the lower arc-shaped mesh clamp 21. Close the lower arc-shaped mesh clamp 21 and the upper arc-shaped clamp 22 and fix them with the hook lock 24. Flip the base support module 1 to expose the mouse's abdomen, and then perform intraperitoneal injection.

[0031] In some embodiments, the basic support module 1 is further provided with an electric winding mechanism 11, on which a rope 12 is provided. The rope 12 is connected to a hook lock 24. The electric winding mechanism 11 is electrically connected to the control module 4 and is controlled by the control module 4. When fixing the upper arc-shaped clamp 22 and the lower arc-shaped mesh clamp 21, the rope 12 is wound around the lower arc-shaped mesh clamp 21 and the hook lock 24 is hung on the upper arc-shaped clamp 22. When the pressure signal exceeds the threshold, the control module controls the electric winding mechanism 11 to release the rope 12, thereby reducing the pressure on the mouse.

[0032] In some embodiments, a camera module 13 is also provided on the basic support module 1. The camera module 13 is used to acquire images of the mouse's abdomen and the syringe. The camera module 13 is connected to the control module 4. The control module 4 runs an injection navigation system. The injection navigation system is configured to: use the lower arc-shaped grid clamp 21 as a reference, calculate the length of the mouse's trunk based on the acquired images of the mouse's abdomen, plan the intraperitoneal injection area and the needle insertion angle of the mouse, calculate the injection site and needle insertion angle of the syringe based on the syringe image, and determine whether the injection site of the syringe falls into the intraperitoneal injection area, and whether the needle insertion angle calculated based on the syringe image falls within the planned needle insertion angle range. Depending on whether all fall within the range, the indicator module 5 issues different reminders. If all fall within the range, the indicator module 5 issues a green reminder. If only the injection site of the syringe falls into the intraperitoneal injection area, the indicator module 5 issues a flashing green reminder. If only the needle insertion angle calculated based on the syringe image falls within the planned needle insertion angle range, a flashing red reminder occurs. If none fall within the range, the indicator module 5 issues a red reminder.

[0033] In some embodiments, the control module 4 is wirelessly connected to a display terminal, which can be a web page or an app. The display terminal shows the image captured by the camera module 13. The injection navigation system is also configured to mark the injection area and needle insertion angle range with guide lines in real time on the display screen. In some application scenarios, the user first logs into the web page and wirelessly connects to the control module 4 via a WiFi module to view the real-time image of the mouse abdomen on the web page. The real-time image also shows the injection area and needle insertion angle range with guide lines. When the syringe needle tip enters the field of view, the injection navigation system analyzes the image to determine whether the injection site of the syringe has fallen into the abdominal cavity. The system identifies the injection area and determines whether the needle insertion angle calculated from the syringe image falls within the planned needle insertion angle range. Once all needle insertion angles fall within the range, the web interface displays "Correct Position" and "Angle Qualified," while indicator module 5 issues a green alert. The operator then performs the injection and clicks the "Start" button on the web interface, at which point the system begins recording. After the injection is completed, pressing the "End" button causes the main control microcontroller to automatically upload data such as the maximum / minimum pressure value, pressure duration, image recognition results, and operation timestamp during the injection process. A record containing all of the above information is generated in the "Experiment Log," which can be exported for subsequent analysis or report writing.

[0034] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. An intelligent intraperitoneal injection auxiliary fixing device for experimental mice, characterized in that, The mouse fixing module is used for fixing a mouse, and the mouse fixing module comprises a lower arc-shaped grid-shaped clamping plate, an upper arc-shaped clamping plate, a connecting piece and a hook lock. The pressure monitoring module is arranged on the lower side of the upper arc-shaped clamping plate and is used for detecting the pressure applied by the upper arc-shaped clamping plate to the mouse. The pressure monitoring module is electrically connected to the control module, and a pressure judgment system is run in the control module.

2. The intelligent intraperitoneal injection auxiliary fixation device for experimental mice according to claim 1, characterized in that, The pressure judgment system is configured to read the pressure signal of the pressure monitoring module, judge whether the pressure is within a threshold range according to the pressure signal, and control the indication module to work. 3.The intelligent intraperitoneal injection auxiliary fixation device for experimental mice according to claim 2, characterized in that, The power module is used for supplying power to the pressure monitoring module, the control module and the indication module.

4. The intelligent intraperitoneal injection auxiliary fixing device for experimental mice according to claim 3, characterized in that, The lower arc-shaped grid-shaped clamping plate and the upper arc-shaped clamping plate both adopt an arc-shaped steel wire grid structure.

5. The intelligent intraperitoneal injection auxiliary fixing device for experimental mice according to claim 2, characterized in that, The upper arc-shaped clamping plate further comprises an arch-shaped part made of a flexible material.

6. The intelligent intraperitoneal injection auxiliary fixation device for experimental mice according to claim 1, characterized in that, The pressure monitoring module adopts a pressure sensing film arranged on the lower side of the arch-shaped part.

7. The intelligent intraperitoneal injection auxiliary fixing device for experimental mice according to claim 6, characterized in that, An electric winding mechanism is further arranged on the base bearing module, a rope is arranged on the electric winding mechanism, the rope is connected to the hook lock, the electric winding mechanism is electrically connected to the control module and is controlled to work by the control module. 8.The intelligent intraperitoneal injection auxiliary fixing device for experimental mice according to claim 7, characterized in that, When the pressure signal exceeds the threshold, the control module controls the electric winding mechanism to release the rope, so as to reduce the pressure on the mouse. A camera module is further arranged on the base bearing module, the camera module is used for collecting images of the mouse's abdomen and an injector, the camera module is connected to the control module, and an injection navigation system is run in the control module. The injection navigation system is configured to: take the lower arc-shaped grid-shaped clamping plate as a reference, calculate the length of the mouse's main stem according to the collected image of the mouse's abdomen, plan the abdominal injection area of the mouse and the needle insertion angle, calculate the injection site and the needle insertion angle of the injector through the image of the injector, and judge whether the injection site of the injector falls within the abdominal injection area and whether the needle insertion angle calculated through the image of the injector falls within the planned needle insertion angle range. The control module is wirelessly connected to a display end which can be a webpage port or an APP software port. The display end displays the picture taken by the camera module. The injection navigation system is further configured to mark the injection area and the guide frame line of the needle insertion angle range in the display picture of the display end in real time.

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