Anti-alcohol composition as well as preparation method and application thereof

Abstract

The invention discloses an anti-alcohol composition as well as a preparation method and application thereof. The anti-alcohol composition is prepared from the following raw materials in parts by weight: 5-30 parts of moringa seed, 5-30 parts of pine pollen, 5-30 parts of ginger, 5-30 parts of sophora flower bud or sophora flower, 5-30 parts of lalang grass rhizome, 5-10 parts of pericarpium citri reticulatae and 2-8 parts of corn peptide. The anti-alcohol composition is scientific and reasonable in formula, takes into account all links (absorption, decomposition and excretion) of alcohol metabolism and main target organs (liver, stomach and blood), and is an excellent composition which treats both symptoms and root causes and emphasizes prevention and treatment.

Description

Technical Field

[0001] This application relates to the field of hangover relief and liver protection technology, specifically to a hangover relief composition, its preparation method, and its uses. Background Technology

[0002] Alcohol is metabolized in the human body through three pathways: the liver, skin, and respiratory system. Approximately 95% of alcohol is metabolized through the liver's enzyme system. Hangover remedies work by enhancing the function of the liver's enzyme system, thus breaking down alcohol. The breakdown of undiluted alcohol in the body primarily relies on two enzymes in the liver's enzyme system: alcohol dehydrogenase and aldehyde dehydrogenase. Alcohol dehydrogenase removes two hydrogen atoms from the alcohol molecule, breaking down ethanol into acetaldehyde. Alcohol dehydrogenase, on the other hand, removes two hydrogen atoms from acetaldehyde, converting it into acetic acid, which is ultimately broken down into carbon dioxide and water.

[0003] If the human body possesses both of these enzymes, alcohol can be broken down more quickly, and the central nervous system will be less affected by alcohol. Everyone in the human body has alcohol dehydrogenase, and the amount is generally equal. However, many people lack aldehyde dehydrogenase. This deficiency of aldehyde dehydrogenase prevents alcohol from being completely broken down into water and carbon dioxide, causing acetaldehyde to remain in the body, leading to symptoms of intoxication such as nausea, vomiting, and dizziness. Therefore, people who are not good drinkers and whose alcohol tolerance is below a reasonable level are considered to have insufficient or complete aldehyde dehydrogenase levels. Even for people who are good drinkers, excessive or rapid consumption beyond the breakdown capacity of these two enzymes can also lead to intoxication.

[0004] Existing hangover remedies mainly work through the following two pathways: promoting... 1. Products that promote alcohol metabolism often contain acetaldehyde dehydrogenase-related substances (such as coenzymes), L-cysteine, and glutathione. After alcohol enters the body, it is first converted to acetaldehyde by alcohol dehydrogenase, then acetaldehyde is converted to acetic acid by acetaldehyde dehydrogenase, and finally broken down into carbon dioxide and water for excretion. These ingredients can help increase the activity of related enzymes, accelerate acetaldehyde metabolism, reduce the accumulation of acetaldehyde in the body, and thus alleviate symptoms of acetaldehyde poisoning such as headache and nausea.

[0005] 2. Liver-protective ingredients mainly include silymarin, curcumin, and B vitamins. Alcohol metabolism primarily occurs in the liver, and excessive drinking damages liver cells. These ingredients can protect liver cell membranes to some extent, reduce oxidative stress damage to the liver, and mitigate short-term alcohol-induced liver damage, but they cannot counteract the chronic damage caused by long-term heavy drinking.

[0006] In addition, traditional Chinese medicine also has some applications in relieving hangovers. However, existing hangover remedies still have issues such as uncertain effectiveness and potential side effects and harms. Summary of the Invention

[0007] This invention provides a hangover relief composition, its preparation method, and its uses. The hangover relief composition has a scientific and reasonable formula, taking into account all aspects of alcohol metabolism (absorption, decomposition, and excretion) and major target organs (liver, stomach, and blood). It is an excellent composition that addresses both the symptoms and the root cause, and combines prevention and treatment.

[0008] To achieve the above objectives, the technical solution of the present invention is as follows: The hangover remedy composition is made from the following raw materials in parts by weight: Moringa seeds 5-30 parts, pine pollen 5-30 parts, ginger 5-30 parts, sophora japonica buds or flowers 5-30 parts, Imperata cylindrica root 5-30 parts, dried tangerine peel 5-10 parts, corn peptide 2-8 parts.

[0009] Preferably, it is made from the following raw materials in parts by weight: Moringa seeds 20-30 parts, pine pollen 10-20 parts, ginger 5-10 parts, sophora japonica buds or flowers 5-10 parts, Imperata cylindrica root 5-10 parts, dried tangerine peel 5-10 parts, corn peptides 2-8 parts.

[0010] The method for preparing the active ingredient of the aforementioned hangover remedy composition includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 6-10 times the total weight of these medicinal materials and decoct 1-3 times, each time for 1-3 hours. Filter, combine the filtrates to obtain the extract. When the extract is concentrated to 80℃, the relative density is 1.2-1.3. Add corn peptide according to the weight proportions and mix well to obtain the active ingredient.

[0011] Preferably, the method for preparing its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add 40-80% ethanol (volume percentage) equivalent to 4-8 times the total weight of these medicinal materials and decoct 1-3 times, each time for 1-3 hours. Filter, combine the filtrates, recover the ethanol, and obtain a concentrated solution. Add corn peptide according to the weight proportions and mix evenly to obtain the active ingredient.

[0012] Preferably, the method for preparing the solution includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 6-10 times the total weight of these herbs and decoct 1-3 times, each time for 1-3 hours. Filter, combine the filtrates to obtain the extract, add corn peptides according to the weight proportions, mix well, and add flavoring agent to obtain the final product.

[0013] Preferably, the preparation method of the ointment includes the following steps: Take the active ingredient, add 1-1.5 times the weight of the active ingredient in colloid, and 1-1.5 times the weight of the active ingredient in rock sugar or honey. Mix well at 50-70℃ and cool to room temperature to obtain the product. The colloid is xanthan gum, donkey-hide gelatin, tortoise shell glue, or deer antler glue.

[0014] Preferably, the method for preparing the granules includes the following steps: Take the active ingredient, add water-soluble dextrin, water-soluble starch, sucrose, or maltodextrin equivalent to 1-2 times the weight of the active ingredient, mix evenly, dry, pulverize, and granulate to obtain the product.

[0015] The present invention also discloses the use of the above-mentioned hangover relief composition in the preparation of liver-protective drugs.

[0016] The present invention also discloses the use of the above-mentioned hangover relief composition in the preparation of a drug for protecting against alcoholic fatty liver.

[0017] The present invention also discloses the use of the above-mentioned hangover relief composition in the preparation of hangover relief drugs.

[0018] Explanation of the formulation of this invention: Principal ingredients: Moringa seeds, pine pollen Moringa seeds Benefits: Modern research shows that moringa seeds are rich in antioxidants (such as isothiocyanates), vitamins, and minerals.

[0019] Explanation: The core of this formula lies in its powerful liver-protecting effect. It can significantly reduce alcohol-induced liver damage, enhance the activity of liver detoxification enzymes, and accelerate alcohol breakdown. Simultaneously, its rich nutrients help alleviate post-drinking weakness and replenish energy.

[0020] Pine pollen: Benefits: Known as the "King of Natural Vitamins", it contains a variety of amino acids, enzymes and trace elements.

[0021] Explanation: Its main functions are to strengthen the spleen and replenish qi, and nourish liver blood. It can significantly enhance the function of the spleen and stomach, fundamentally improving the body's ability to metabolize fluids and resist alcohol toxicity. At the same time, it can promote liver cell activity and regeneration, working synergistically with moringa seeds to form a powerful "liver protection umbrella."

[0022] Ingredients: Ginger, corn peptides Ginger: Efficacy: Warms the middle jiao and stops vomiting, relieves exterior syndromes and dispels cold.

[0023] Explanation: Ginger is a highly effective antiemetic, capable of combating nausea, vomiting, and stomach discomfort caused by alcohol toxicity damaging the spleen and stomach. Its pungent and dispersing properties can also help disperse the effects of alcohol, relieving dizziness and a heavy head.

[0024] Corn peptides: Efficacy: Rich in amino acids (alanine, glutamic acid), it can significantly increase the concentration of alanine and leucine in the blood and activate alcohol dehydrogenase and acetaldehyde dehydrogenase.

[0025] Explanation: This is the key targeted ingredient in this formula that directly accelerates alcohol metabolism. It can efficiently convert toxic acetaldehyde into harmless acetic acid, thereby fundamentally reducing the accumulation of acetaldehyde in the body. It is the main remedy for relieving symptoms of acetaldehyde poisoning such as headache, facial flushing, and palpitations.

[0026] Adjuvant herbs: Sophora japonica flowers, Imperata cylindrica root, dried tangerine peel Sophora japonica buds / flowers: Efficacy: Cools the blood and stops bleeding, clears liver heat and reduces fire.

[0027] Explanation: Alcohol poisoning enters the blood, easily generating blood heat. Sophora japonica flowers are cold in nature and can clear heat toxins from the blood, effectively relieving symptoms such as facial flushing, red eyes, headache, and high blood pressure after drinking. Its rutin and other components can also enhance capillary resilience.

[0028] Imperata cylindrica root: Efficacy: Clears heat and promotes diuresis, generates fluids and quenches thirst.

[0029] Explanation: This formula targets symptoms such as dry mouth, thirst, and scanty dark urine caused by alcohol toxicity depleting body fluids. Through its diuretic effect, it guides the damp-heat alcohol toxicity out through urine, providing an outlet for the pathogenic factors—a classic example of "giving the pathogenic factors a way out."

[0030] Dried tangerine peel: Efficacy: Regulates Qi and strengthens the spleen, dries dampness and harmonizes the middle Jiao.

[0031] Explanation: This formula is used to regulate the Qi of the spleen and stomach when it is obstructed by alcohol and dampness. It can promote the flow of Qi and eliminate dampness, relieve abdominal distension, and improve appetite. When combined with ginger, it helps to restore the ascending and descending functions of the spleen and stomach.

[0032] The compatibility characteristics of the formula in this invention are corroborated by modern pharmacology: 1. Prevention and treatment combined: Pre-drinking prevention: Taking pine pollen and moringa seeds in advance can enhance the function of the spleen, stomach and liver, playing a role in "preventing drunkenness before it happens".

[0033] Treatment after drinking: Corn peptides, ginger, and Imperata cylindrica root can quickly relieve various acute symptoms after drinking and speed up sobering up.

[0034] 2. Dual pathways: metabolism and excretion Accelerate metabolism (addressing the root cause): Corn peptides are the core ingredient, directly improving the efficiency of ethanol decomposition.

[0035] Promotes excretion (treating the symptoms): Imperata cylindrica root promotes urination, while tangerine peel and ginger regulate the spleen and stomach's Qi mechanism, working together to expel toxins that have been metabolized and not absorbed from the body.

[0036] 3. Protection and repair of multiple targets: Liver protection: Moringa seeds and pine pollen form the core ingredients, providing comprehensive liver protection.

[0037] Gastrointestinal protection: Ginger and dried tangerine peel can relieve gastrointestinal discomfort.

[0038] Vascular and nervous system protection: Sophora japonica flowers clear heat from the blood and relieve headaches and facial flushing.

[0039] 4. Combining Chinese and Western approaches, with a clear mechanism: This formula cleverly combines the "holistic conditioning" of traditional Chinese medicine (ginger, tangerine peel, etc.) with the "targeted action" of modern extracts (corn peptides, moringa seeds), making the mechanism of sobering up and relieving hangovers very clear and scientific.

[0040] This invention can serve as a highly effective health-promoting formula or medicine for relieving hangovers, protecting the liver, and invigorating the spleen. Its design concept is clear, taking into account all stages of alcohol metabolism (absorption, breakdown, and excretion) and major target organs (liver, stomach, and blood). It is an excellent formula that addresses both the symptoms and root cause, emphasizing both prevention and treatment, and has promising application prospects. Attached Figure Description

[0041] Figure 1 This is a graph showing the relative staining intensity of liver fat at different concentrations in Experiment Example 2. Figure 2 This is a bar chart showing the relative staining intensity of liver fat at different concentrations in the samples from Experiment Example 2. Note: Compared with the model control group, **** (P<0.0001) showed a highly significant difference; *** (P<0.001) showed a significant difference; ** (P<0.01) showed a significant difference; * (P<0.05) showed a significant difference; Compared with the blank control group, #### (P<0.0001) showed a highly significant difference; ### (P<0.001) showed a significant difference; ## (P<0.01) showed a significant difference; # (P<0.05) showed a significant difference. Detailed Implementation

[0042] The present invention will now be described in detail through specific embodiments. Example 1

[0043] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 5kg, pine pollen 5kg, ginger 5kg, sophora japonica flowers 5kg, Imperata cylindrica root 5kg, dried tangerine peel 5kg, corn peptide 2kg.

[0044] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 6 times the total weight of these herbs and decoct once, each time for 1 hour. Filter, combine the filtrates to obtain the extract. Concentrate the extract to 80℃ to obtain a relative density of 1.2. Add corn peptides according to the weight proportions and mix well to obtain the active ingredient.

[0045] The preparation method of the ointment includes the following steps: Take the active ingredient, add xanthan gum equivalent to 1 times the weight of the active ingredient, and honey equivalent to 1 times the weight of the active ingredient, mix them evenly at 50℃, and cool to room temperature to obtain the product. Example 2

[0046] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 30kg, pine pollen 30kg, ginger 30kg, sophora japonica buds or flowers 30kg, Imperata cylindrica root 30kg, dried tangerine peel 10kg, corn peptides 8kg.

[0047] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 10 times the total weight of these medicinal materials and decoct three times, each time for 3 hours. Filter and combine the filtrates to obtain the extract. When the extract is concentrated to 80℃, the relative density is 1.3. Add corn peptides according to the weight proportions and mix well to obtain the active ingredient.

[0048] The preparation method of the ointment includes the following steps: Take the active ingredients, add 1.5 times the weight of donkey-hide gelatin and 1.5 times the weight of rock sugar, mix them evenly at 50℃, and cool to room temperature to obtain the final product. Example 3

[0049] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 25kg, pine pollen 15kg, ginger 10kg, sophora japonica buds 10kg, imperata root 10kg, dried tangerine peel 8kg, corn peptides 5kg.

[0050] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 8 times the total weight of these medicinal materials and decoct twice, each time for 2 hours. Filter and combine the filtrates to obtain the extract. Concentrate the extract to 80℃ to obtain a relative density of 1.25. Add corn peptides according to the weight proportions and mix well to obtain the active ingredient.

[0051] Take the active ingredient, add tortoise shell glue (1.2 times the weight of the active ingredient) and honey (1-1.2 times the weight of the active ingredient), mix evenly at 50-70℃, and cool to room temperature to obtain the product. Example 4

[0052] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 20kg, pine pollen 10kg, ginger 5kg, sophora japonica buds 5kg, imperata cylindrica root 5kg, dried tangerine peel 5kg, corn peptides 2kg.

[0053] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add 60% ethanol (volume percentage) equivalent to 6 times the total weight of these herbs and decoct twice, 3 hours each time. Filter, combine the filtrates, recover the ethanol, and obtain a concentrated liquid. Add corn peptide according to the weight proportions and mix well to obtain the active ingredient.

[0054] The preparation method of the ointment includes the following steps: Take the active ingredient, add 1.1 times the weight of the active ingredient in deer antler glue, and 1.2 times the weight of the active ingredient in rock sugar or honey. Mix well at 55°C and cool to room temperature to obtain the final product. Example 5

[0055] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 30kg, pine pollen 20kg, ginger 10kg, sophora japonica flowers 10kg, Imperata cylindrica root 10kg, dried tangerine peel 10kg, corn peptides 8kg.

[0056] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add 40% ethanol (volume percentage) equivalent to 4 times the total weight of these herbs and decoct once for 1 hour each time. Filter, combine the filtrates, recover the ethanol, and obtain a concentrated liquid. Add corn peptide according to the weight proportions and mix well to obtain the active ingredient.

[0057] The preparation method of its granules includes the following steps: Take the active ingredient, add water-soluble dextrin equivalent to one times the weight of the active ingredient, mix well, dry, pulverize, and granulate to obtain the final product. Example 6

[0058] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 25kg, pine pollen 15kg, ginger 8kg, sophora japonica buds 7kg, imperata root 6kg, dried tangerine peel 77kg, corn peptides 5kg.

[0059] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add 80% ethanol (8 times the total weight of these herbs by volume) and decoct three times, each time for 3 hours. Filter, combine the filtrates, recover the ethanol, and obtain a concentrated liquid. Add corn peptide according to the weight proportions and mix well to obtain the active ingredient.

[0060] The preparation method of its granules includes the following steps: Take the active ingredient, add water-soluble starch equivalent to twice the weight of the active ingredient, mix evenly, dry, pulverize, and granulate to obtain the product. Example 7

[0061] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 20kg, pine pollen 12kg, ginger 6kg, sophora japonica flowers 6kg, Imperata cylindrica root 8kg, dried tangerine peel 8kg, corn peptide 4kg.

[0062] The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica flowers, Imperata cylindrica root, and dried tangerine peel according to the weight ratio. Add water equivalent to 8 times the total weight of these medicinal materials and decoct twice, each time for 2 hours. Filter and combine the filtrates to obtain the extract. When the extract is concentrated to 80℃, the relative density is 1.26. Add corn peptide according to the weight ratio and mix well to obtain the active ingredient.

[0063] The preparation method of its granules includes the following steps: Take the active ingredient, add sucrose equivalent to 1.5 times the weight of the active ingredient, mix well, dry, pulverize, and granulate to obtain the final product. Example 8

[0064] The hangover relief composition of this embodiment is made from the following raw materials: Moringa seeds 25kg, pine pollen 15kg, ginger 10kg, sophora japonica buds 10kg, imperata root 10kg, dried tangerine peel 8kg, corn peptides 5kg.

[0065] The preparation method of its solution includes the following steps: Weigh out the following ingredients by weight: Moringa seeds, pine pollen, ginger, Sophora japonica buds, Imperata cylindrica root, and dried tangerine peel. Add water equal to 8 times the total weight of these herbs and decoct twice, 2 hours each time. Filter the decoction, combine the filtrates to obtain the extract. Add corn peptides by weight, mix well, and add 20% sucrose by weight of the extract to obtain the final product.

[0066] Experiment Example 1: Hangover Relief Experiment 1. Sample preparation: The composition solution prepared according to Example 8 (containing approximately 0.6 g of medicinal material per ml) 2. Experimental Procedure and Methods: Test subjects: People who drink alcohol, aged 24-72.

[0067] Grouping: Control group (11 people), Sample group (12 people), Blank group (4 people) Dosage: The control group drank alcohol and then took 180ml of purified water; the sample group drank alcohol and then took 180ml of the solution from Example 8; the blank group did not drink alcohol and took purified water.

[0068] Test method: Rinse your mouth with purified water after drinking alcohol and then measure the value.

[0069] 1) Once the amount of alcohol and water reaches 1 liang (50ml), the alcohol content value is M1; 2) The alcohol content value M2 is measured 6 minutes after drinking the solution or purified water. 3) Alcohol content value M3 after 15 minutes; 4) After the amount of alcohol and water reaches 2 liang (approximately 100ml), the alcohol content value is M4; 5) The alcohol content value M5 is measured 15 minutes after drinking the solution or purified water. 6) After the amount of alcohol and water reaches 4 liang (200ml), the alcohol content value is M6; 7) Alcohol content value M7 after 15 minutes; 8) The alcohol content value M8 after 30 minutes.

[0070] Testing equipment: Breath alcohol content detector, model: Eagle-1, measurement range BrAC (0-2.5) mg / L, manufacturer: Shenzhen Keyun Technology Co., Ltd.; the maximum display value of this device is 500.

[0071] Table 1. Statistical analysis of experimental results: test substance Subject number gender age Before use After use After use After use After use After use After use After use After use 1 tael 1 tael 1 tael 2 liang 2 liang 4 liang 4 liang 4 liang M1 M2 M3 M4 M5 M6 M7 M8 Blank group 1 female 45 0 0 0 0 0 0 0 0 0 Blank group 2 male 27 0 0 0 0 0 0 0 0 0 Blank group 3 male 38 0 0 0 0 0 0 0 0 0 Blank group 4 male 51 0 0 0 0 0 0 0 0 0 control group 5 male 33 0 350 352 341 500 500 500 500 500 control group 6 male 50 0 298 285 274 500 500 500 500 500 control group 7 male 52 0 274 367 332 500 500 500 500 500 control group 8 male 57 0 336 263 240 500 448 500 467 451 control group 9 male 43 0 295 274 248 500 500 500 500 475 control group 10 male 63 0 330 330 317 500 500 500 500 500 control group 11 male 58 0 362 322 308 500 478 500 481 475 control group 12 male 63 0 390 367 330 500 500 500 500 500 control group 13 male 49 0 341 319 302 500 497 500 478 432 control group 14 female 45 0 362 330 289 500 483 500 500 483 control group 15 female 51 0 346 342 336 500 500 500 476 447 Sample group 16 male 72 0 195 6 0 214 12 320 36 4 Sample group 17 male 56 0 84 0 0 110 6 287 13 0 Sample group 18 male 53 0 93 0 0 201 14 362 42 6 Sample group 19 male 55 0 86 2 0 184 0 295 8 0 Sample group 20 male 48 0 120 4 0 203 18 305 31 17 Sample group 21 male 31 0 122 10 0 186 2 297 6 0 Sample group 22 male 24 0 150 0 0 197 0 283 0 0 Sample group 23 male 53 0 280 9 2 240 21 312 27 14 Sample group 24 male 51 0 132 14 0 184 1 264 17 6 Sample group 25 male 54 0 98 7 0 168 0 241 13 0 Sample group 26 female 46 0 86 0 0 203 2 302 36 3 Sample group 27 female 54 0 110 0 0 186 0 287 15 0 ; As shown in Table 1 above, the hangover relief effect of the sample group was significantly better than that of the control group.

[0072] Experiment Example 2: Zebrafish Liver Protection Experiment 1. Test substance pretreatment methods Sample 250113-01: Take the active ingredient prepared in Example 3; Sample 250113-02: Take kudzu root and add water equivalent to 8 times the total weight of kudzu root, decoct twice, each time for 2 hours, filter, combine the filtrates, and concentrate to a relative density of 1.25 at 80℃; Weigh 0.0070g of sample 250113-01 and add it to 7.000mL of embryo buffer to prepare a stock solution of 1g / L. Dilute the stock solution to the corresponding concentration for testing. Weigh 0.0100g of sample 250113-02 and add it to 10.000mL of embryo buffer to prepare a stock solution of 1g / L. Dilute the stock solution to the corresponding concentration for testing.

[0073] Table 2. Specific information about the experimental samples: Sample number Sample Name Replenish 250113-01 Active ingredient prepared in Example 3 The test concentrations were 2.5 mg / L, 5 mg / L, and 10 mg / L. 250113-02 Pueraria lobata extract The test concentrations were 2.5 mg / L, 5 mg / L, and 10 mg / L. .

[0074] 2. Detection Principle Alcoholic fatty liver disease (ALD) is a condition caused by excessive alcohol consumption, with simple hepatic steatosis, progressive fibrosis, and cirrhosis being the main indicators. Therefore, inhibiting hepatic fat accumulation is a crucial indicator for evaluating the therapeutic efficacy of products against ALD. Zebrafish share a high genetic similarity to humans in terms of disease, and intact liver function can be detected in zebrafish at 3 days old, allowing for observation of hepatic fat accumulation through staining. Therefore, this project used zebrafish to construct an ALD model, stained zebrafish liver fat with saturated Oil Red O, and statistically analyzed the hepatic fat deposition area to reflect the ability of the tested sample to inhibit hepatic fat deposition, thereby evaluating the protective efficacy of the tested sample against ALD.

[0075] 3. Laboratory animals and environment (1) Conditions for raising zebrafish In the breeding and specialized aquaculture system for adult wild zebrafish, the pH range is 7.0-8.0, the conductivity range is 500-800 μS / cm, the room temperature range is 26℃±1℃, the water temperature range is 28℃±1℃, and the photoperiod is 14h light and 10h darkness.

[0076] (2) Preparation of experimental embryos The afternoon before collecting the eggs, transfer the 3-18 month old male and female zebrafish, which have already started feeding, to the breeding tank and insert a partition. The next morning, between 9:00 and 9:30, remove the partition in the middle of the breeding tank. Collect the embryos 15 minutes after removing the partition. Place the embryos in... When embryos reach 5 dpf in a constant temperature incubator at (28±0.5℃) with added embryo buffer (see Table 2 for embryo buffer preparation method), select normally developing embryos for experiments.

[0077] Table 3. Preparation method of embryo buffer solution: Embryo buffer formulation NaCl 15.75 g NaHCO3 0.9 g KCl 0.25 g CaCl2 0.52875 g , Add the ingredients to the measuring cups according to the table, and bring the volume to 4.5L with ultrapure water. Filter using a vacuum filter (0.2μM pore size), sterilize using an autoclave, and store at room temperature.

[0078] 4. Experimental Methods (1) Experimental grouping This experiment consisted of 1+1+1+3 groups, namely, a blank control group, a model control group, a positive control group, and three groups with different sample concentrations.

[0079] (2) Group design Use 60 mm culture dishes, with 3 dishes per group and 20 embryos per dish. See Table 4.

[0080] Table 4 Dosage Design Table: Group concentration Quantity per dish (tails) Number of dishes Total (tails) Blank control group 0.25 mg / mL fish meal 20 3 60 Model control group 0.25 mg / mL fish meal solution + 0.5 mg / mL egg yolk powder solution + 0.1% ethanol solution 20 3 60 Positive control group 0.0625mM bile acid solution 20 3 60 2.5 mg / L 20 3 60 250113-01 5 mg / L 20 3 60 10 mg / L 20 3 60 2.5 mg / L 20 3 60 250113-02 5 mg / L 20 3 60 10 mg / L 20 3 60 .

[0081] (3) Experimental procedure Wild-type zebrafish embryos at 5 dpf were collected and grouped according to the group design in section 4.2, with 60 embryos per group. The blank control group received 10 mL of embryo buffer containing 0.25 mg / mL fish meal per dish. The model control group received 10 mL of high-fat diet combined with an alcohol solution (0.25 mg / mL fish meal solution + 0.5 mg / mL egg yolk powder solution + 0.1% ethanol solution) per dish. The positive control group received 10 mL of bile acid solution (0.25 mg / mL fish meal solution + 0.5 mg / mL egg yolk powder solution + 0.1% ethanol solution + 0.0625 mM bile acid solution) per dish. The treatment group received 10 mL of a mixture of high-fat diet and the corresponding concentration of 250113-01 or 250113-02 solution per dish. All were incubated in a constant temperature incubator at (28 ± 0.5℃).

[0082] Incubate in a constant temperature incubator until 9 days post-fertilization (dpf). Replace half of the solution in the culture dish every 24 hours to maintain consistent concentration of all components. At 9 days old, replace the solution in each group with fish embryo culture medium and fast for one day.

[0083] After fasting and cleaning, the liver was fixed and stained with Oil Red O to remove fat.

[0084] The liver was placed under a stereomicroscope for microscopic imaging, and the intensity of liver fat staining was statistically analyzed.

[0085] (4) Data analysis and processing: ImageJ was used to statistically analyze the hepatic fat staining intensity of zebrafish. The statistical results were imported into Excel for data processing, and the mean and standard error (SEM) of each group were calculated. All results are expressed as mean ± standard error (Mean ± SEM). GraphPad Prism 8 was used for statistical analysis, and differences between groups were analyzed using a t-test. Generally, P < 0.05 was considered statistically significant.

[0086] 5. Experimental Results: The intensity of fat staining in the liver of zebrafish samples is shown in the figure. Figure 1 , Figure 2 And Table 5.

[0087] Table 5. Statistical results of relative staining intensity of liver fat in zebrafish: Group concentration Relative staining intensity of liver fat (X̅±) Liver fat deposition rate % Liver fat deposition rate % Blank control group 0.25 mg / mL fish meal 1.00±0.06 / / Model control group 0.25 mg / mL fish meal solution + 0.5 mg / mL egg yolk powder solution + 0.1% ethanol solution 1.44±0.07 / #### Positive control group 0.0625 mM bile acid solution 1.22±0.06 15 * 250113-01 2.5 mg / L 1.29±0.05 10 ns 250113-01 5 mg / L 1.22±0.06 15 * 250113-01 10 mg / L 1.14±0.05 21 ** 250113-02 2.5 mg / L 1.73±0.08 -20 ns 250113-02 5 mg / L 1.76±0.10 -23 ns 250113-02 10 mg / L 1.24±0.05 14 * .

[0088] Note: Compared with the model control group, **** (P<0.0001) showed a highly significant difference; *** (P<0.001) showed a significant difference; ** (P<0.01) showed a significant difference; * (P<0.05) showed a significant difference; Compared with the blank control group, #### (P<0.0001) showed a highly significant difference; ### (P<0.001) showed a significant difference; ## (P<0.01) showed a significant difference; # (P<0.05) showed a significant difference.

[0089] 5. Experimental Conclusions Based on the results of this experiment: Under the experimental conditions, when the concentrations of 250113-01 were 5 mg / L and 10 mg / L, and the concentration of 250113-02 was 10 mg / L, the depth of liver fat staining in zebrafish was significantly reduced compared to the model control group. This indicates that the samples have a significant improving effect on the zebrafish alcoholic liver model induced by a high-fat diet combined with alcohol, suggesting that the samples at these concentrations can exert a liver-protecting and alcohol-detoxifying effect by reducing the depth of liver fat accumulation. In particular, 250113-01, representing the composition of this invention, has a good liver-protecting and alcohol-detoxifying effect at all concentrations, and this effect is significantly enhanced with increasing concentration.

Claims

1. A hangover remedy composition, characterized in that, Made from the following parts by weight of raw materials: Moringa seeds 5-30 parts, pine pollen 5-30 parts, ginger 5-30 parts, sophora japonica buds or flowers 5-30 parts, Imperata cylindrica root 5-30 parts, dried tangerine peel 5-10 parts, corn peptide 2-8 parts.

2. The hangover relief composition according to claim 1, characterized in that, It is made from the following ingredients in parts by weight: 20-30 parts moringa seeds, 10-20 parts pine pollen, 5-10 parts ginger, 5-10 parts sophora japonica buds or flowers, 5-10 parts Imperata cylindrica root, 5-10 parts dried tangerine peel, and 2-8 parts corn peptides.

3. The hangover relief composition according to claim 1, characterized in that, The preparation method of its active ingredient includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 6-10 times the total weight of these medicinal materials and decoct 1-3 times, each time for 1-3 hours. Filter, combine the filtrates to obtain the extract. When the extract is concentrated to 80℃, the relative density is 1.2-1.

3. Add corn peptide according to the weight proportions and mix well to obtain the active ingredient.

4. The method for preparing the active ingredient of the hangover relief composition according to claim 1 includes the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add 40-80% ethanol (volume percentage) equivalent to 4-8 times the total weight of these medicinal materials and decoct 1-3 times, each time for 1-3 hours. Filter, combine the filtrates, recover the ethanol, and obtain a concentrated solution. Add corn peptide according to the weight proportions and mix evenly to obtain the active ingredient.

5. The hangover relief composition according to claim 1 or 2, wherein the preparation method of the solution comprises the following steps: Weigh out Moringa seeds, pine pollen, ginger, Sophora japonica buds or flowers, Imperata cylindrica root, and dried tangerine peel according to the weight proportions. Add water equivalent to 6-10 times the total weight of these herbs and decoct 1-3 times, each time for 1-3 hours. Filter, combine the filtrates to obtain the extract, add corn peptides according to the weight proportions, mix well, and add flavoring agent to obtain the final product.

6. The hangover relief composition according to claim 2 or 33, wherein the ointment is prepared by the following steps: Take the active ingredient, add 1-1.5 times the weight of the active ingredient in colloid, and 1-1.5 times the weight of the active ingredient in rock sugar or honey. Mix well at 50-70℃ and cool to room temperature to obtain the product. The colloid is xanthan gum, donkey-hide gelatin, tortoise shell glue, or deer antler glue.

7. The method for preparing the granules of the hangover relief composition according to claim 1 or 2 comprises the following steps: Take the active ingredient, add water-soluble dextrin, water-soluble starch, sucrose, or maltodextrin equivalent to 1-2 times the weight of the active ingredient, mix evenly, dry, pulverize, and granulate to obtain the product.

8. Use of the hangover relief composition according to any one of claims 1-7 in the preparation of a liver-protective medicament.

9. Use of the hangover-relieving composition according to any one of claims 1-7 in the preparation of a medicament for protecting against alcoholic fatty liver disease.

10. Use of the hangover relief composition according to any one of claims 1-7 in the preparation of a hangover relief medicine.

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