Aconitine poisoning biomarker as well as screening method and application thereof

By screening aconitine poisoning biomarkers using network toxicology and untargeted metabolomics techniques, and using an increased phenylalanine/tyrosine ratio and a decreased tyrosine/β-phenylethylamine ratio as diagnostic criteria, the problem of identifying poisoning by Aconitum species in traditional Chinese medicine has been solved, enabling rapid and accurate poisoning determination.

CN121933641APending Publication Date: 2026-04-28SHANXI MEDICAL UNIV
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Patent Information

Application Number
CN202512003329.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-29
Publication Date
2026-04-28

AI Technical Summary

Technical Problem

Existing technologies make it difficult to quickly and accurately identify biomarkers of aconite poisoning, which hinders the resolution of cases in forensic examinations. In particular, due to the instability and rapid metabolism of aconitine, routine pathological examinations often fail to reveal any special signs, posing a challenge to forensic professionals.

Method used

By screening aconitine poisoning biomarkers using network toxicology and untargeted metabolomics techniques, constructing topological network analysis, and combining high-performance liquid chromatography-tandem mass spectrometry to detect changes in the ratios of phenylalanine, tyrosine, and β-phenylethylamine, evidence of poisoning from aconitine-related traditional Chinese medicine ingested before death was provided.

Benefits of technology

It reduces the impact of individual differences, enables rapid and accurate determination of aconitine poisoning, and is applicable to research on poisoning by multiple routes and targets, especially in cases of poisoning by natural drugs, where it has low cost and high accuracy.

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Abstract

The invention belongs to the technical field of network toxicology and metabonomics detection, and provides an aconitine poisoning biomarker as well as a screening method and application thereof, the aconitine poisoning biomarker is phenylalanine, tyrosine and beta-phenylethylamine, specifically, the ratio of phenylalanine to tyrosine is increased, and the ratio of tyrosine to beta-phenylethylamine is decreased. According to the invention, the relative change of increase of phenylalanine / tyrosine ratio and decrease of tyrosine / beta-phenylethylamine ratio is simultaneously used as a judgment standard of related evidence of poisoning caused by taking the aconitum traditional Chinese medicine before life, so that the influence caused by individual difference is greatly reduced; the method disclosed by the invention is suitable for most organic toxic drugs with multi-path and multi-target toxic effects, particularly has a great development prospect in research on poisoning of natural drugs and toxicants, and can be used for rapidly and accurately determining poisoning biomarkers with lower cost through network toxicology and molecular docking technologies; the reference and the application value can be well provided for the prenatal poisoning judgment of the natural medicine poison.
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