Aroma-enhancing paper filter stick based on bioelectric fermentation and preparation method of aroma-enhancing paper filter stick

Abstract

The invention belongs to the technical field of tobacco processing, and provides an aroma-enhanced paper filter stick based on bioelectric fermentation and a preparation method thereof.The aroma-enhanced paper filter stick comprises modified high-permeability forming paper and effective aroma components fixed to the modified high-permeability forming paper through a loading and drying process and derived from a tobacco concentrated solution, the surface of the modified high-permeability forming paper is coated with an adsorption enhancer prepared by mixing polyvinyl alcohol and nano silicon dioxide as raw materials; the tobacco concentrated solution is prepared from waste tobacco as a raw material through bioelectric fermentation in a weak electric field. The aroma characteristic of the tobacco concentrated solution is directionally optimized through the bioelectric fermentation technology, the brand-new aroma-enhanced paper filter stick is prepared in cooperation with the modified high-permeability forming paper preparation technology, and the problems that a traditional filter stick is poor in aroma enhancement effect, insufficient in aroma coordination, high in production energy consumption and poor in environmental protection property can be solved.

Description

Technical Field

[0001] This application relates to the field of tobacco processing technology, and in particular to a flavor-enhancing paper filter rod based on bioelectrofermentation and its preparation method. Background Technology

[0002] As a key component of cigarettes, the filter stick plays a vital role in improving cigarette quality and meeting consumers' demands for unique flavors and tastes. Traditional flavoring paper filter sticks mainly fall into two categories:

[0003] The first type involves directly adding granules of the flavoring itself. This method results in a weak flavor enhancement effect, easily introduces off-odors, and has a short-lasting aroma. For example, patent application CN121242290A discloses a flavoring paper filter rod for tobacco, a binary flavoring paper filter rod for tobacco, its preparation method, and its application. The method for preparing the flavoring paper filter rod includes sequentially frying, fermenting, and steam-exploding fresh tea leaves; drying, pulverizing, and sieving the steam-exploded tea leaves to obtain a solid flavoring agent; and mixing the solid flavoring agent with cellulose diacetate tow for tobacco to prepare the flavoring paper filter rod. While the flavoring paper filter rods prepared by this method have a rich aroma, they all introduce off-odors.

[0004] The second type involves placing flavorings in microcapsules within the filter rod. While this achieves a certain degree of sustained release, the aroma and tobacco flavor are poorly coordinated, and the evenness of release per puff needs improvement. This is because the microcapsules release flavorings under mechanical pressure or heat within the filter rod. However, the high-temperature smoke from cigarette combustion differs significantly from the filter rod's temperature, causing the flavoring release timing to be out of sync with the mainstream smoke components, resulting in an overly strong aroma at the beginning and a bland finish. Alternatively, many microcapsule flavorings (such as isolated menthol and synthetic fruit flavorings) have low boiling points and high volatility. Although easily released, they mask the natural sweet and caramelized aroma of tobacco, disrupting the overall flavor balance. Furthermore, some wall materials (such as gelatin-polysaccharide systems) lack stability in the acidic, alkaline, or humid environments of cigarette filters, leading to excessively rapid initial release and an inability to achieve a sustained and coordinated release per puff.

[0005] Meanwhile, reducing tar and harm has become a core research and development issue in the cigarette industry. Technological iterations have driven the tar content of mainstream products down from the traditional 10mg-12mg to below 8mg, with some high-end products even achieving ultra-low tar control of 3mg. However, the process of reducing tar has also brought about prominent problems such as weak aroma and insufficient strength, placing higher demands on the aroma retention and release control of filter rods. Therefore, in light of consumers' demand for upgraded cigarette quality and the industry's urgent orientation towards green production, there is an urgent need to develop an innovative technology that can overcome the shortcomings of traditional aroma enhancement methods, adapt to ultra-low tar scenarios, and comply with environmental protection principles. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a flavor-enhancing paper filter rod based on bioelectric fermentation and its preparation method, which can solve the problems of poor flavor enhancement effect, insufficient aroma coordination, high production energy consumption, and poor environmental performance of traditional filter rods.

[0007] To achieve the above and related objectives, the present invention employs the following technical means:

[0008] The first aspect of the present invention provides a flavoring paper filter rod based on bioelectrofermentation, which includes modified high-permeability forming paper and effective aroma-enhancing components derived from tobacco concentrate fixed thereon by a loading and drying process. The modified high-permeability forming paper is coated with an adsorption enhancer made from a mixture of polyvinyl alcohol and nano-silica. The tobacco concentrate is prepared by bioelectrofermentation of waste tobacco under a weak electric field.

[0009] Furthermore, the basis weight of the modified high-transparency forming paper is 20 g / m³. 2 ~28g / m 2 The air permeability is 8000CU~12000CU, and the tensile strength is 2.5N / mm. 2 ~3.5N / mm 2 Furthermore, the coating amount of the adsorption enhancer is 2 g / m². 2 ~5g / m 2 .

[0010] Furthermore, the aroma-enhancing paper filter rod has a circumference of 16.9mm~24.1mm, a length of 100mm~120mm, a pressure drop of 4000Pa~7500Pa, and a hardness of 80%~90%.

[0011] A second aspect of the present invention provides a method for preparing a flavoring paper filter rod based on bioelectrofermentation, comprising the following steps:

[0012] (1) After pretreatment of waste tobacco, it is mixed with deionized water to prepare a slurry;

[0013] (2) Using slurry as fermentation substrate, inoculate with compound strains and carry out bioelectric fermentation under a weak electric field to obtain fermentation broth; centrifuge, filter, concentrate and purify the fermentation broth to obtain tobacco concentrate.

[0014] (3) An adsorption reinforcing agent is coated on the surface of the high-transparency forming paper to obtain modified high-transparency forming paper, wherein the adsorption reinforcing agent is made by mixing polyvinyl alcohol and nano-silica as raw materials;

[0015] (4) The tobacco concentrate is added to the surface of the modified high-permeability forming paper by spraying or impregnation, and then rolled and cured to prepare the aroma-enhancing paper filter rod.

[0016] Further, in step (1), waste tobacco is pretreated to obtain tobacco powder, and the solid-liquid ratio of tobacco powder to deionized water is 1:(5~10).

[0017] Furthermore, the fermentation process of the fermentation broth in step (2) satisfies at least one of the following conditions (a) to (d):

[0018] (a) The compound strain is Bacillus subtilis and lactic acid bacteria, and the colony count ratio of Bacillus subtilis to lactic acid bacteria is (1~2):1;

[0019] (b) The inoculum size of the compound strain is 5% to 10% of the fermentation substrate mass;

[0020] (c) The weak electric field is 0.1V / cm to 0.3V / cm;

[0021] (d) The fermentation time is 7 to 10 days.

[0022] Furthermore, the tobacco concentrate preparation process in step (2) satisfies at least one of the following conditions (e) to (f):

[0023] (e) Concentration is achieved by sequentially passing the material through an ultrafiltration membrane and a nanofiltration membrane, until the solid content is 20% to 30%;

[0024] (f) Purification includes sequentially passing the material through an activated carbon chromatography column and an ion exchange resin column.

[0025] Furthermore, in step (3), the mass ratio of polyvinyl alcohol to nano silica is (3~5):1.

[0026] Furthermore, in step (4), when using a spraying process, the amount of tobacco concentrate added is 10 g / m³. 2 ~15g / m 2 .

[0027] Furthermore, in step (4), when the impregnation process is used, the basis weight of the modified high-permeability forming paper loaded with tobacco concentrate after impregnation is 34 g / m³. 2 ~39g / m 2 .

[0028] The beneficial technical effects of this invention are as follows:

[0029] This invention overcomes the technical bottlenecks of traditional direct addition of flavorings and microencapsulation, innovatively designing an integrated system of bio-electro-fermentation for targeted flavor enhancement and synergistic flavor consolidation with adsorption enhancers. By applying a weak electric field of 0.1V / cm to 0.3V / cm to the fermentation system, combined with the synergistic fermentation of a composite strain of Bacillus subtilis and lactic acid bacteria, the aroma-producing metabolic pathways of the strains can be activated, directionally promoting the synthesis of aroma-producing substances such as terpenes and phenols in tobacco, and inhibiting the generation of impurities such as hydrogen sulfide and amines. The content of aroma-producing substances is increased by 65% ​​to 80% compared to traditional fermentation. However, excessively high electric field strength will damage the microbial cell membrane, leading to metabolic stagnation and the production of irritating byproducts. Simultaneously, a composite adsorption enhancer of polyvinyl alcohol and nano-silica is used, with complementary functions: the hydroxyl groups of polyvinyl alcohol form hydrogen bonds with aroma components to strengthen initial adsorption, while the porous structure of nano-silica constructs a "micro-storage cellar" to delay release, effectively solving the defects of traditional single adsorbents, which have rapid adsorption and loss. The adsorption enhancer of this invention can be uniformly adhered to the surface of high-transparency forming paper by doctor blade coating, fundamentally solving the industry problem of easy agglomeration of single nanoparticles and uneven coating; at the same time, the film-forming properties of polyvinyl alcohol can significantly improve the tensile strength of modified high-transparency forming paper, thereby achieving a balance between adsorption and fragrance enhancement performance and mechanical processing performance.

[0030] This invention uses waste tobacco as raw material to prepare tobacco concentrate through bio-electro-fermentation, replacing high-quality tobacco raw materials, thereby realizing the recycling of waste. Combined with the optimized process of modified high-permeability forming paper, it further reduces production energy consumption and pollutant emissions, thus aligning with the industry's green production orientation.

[0031] It should be understood that the above general description and the following detailed description are exemplary and explanatory only, and do not limit this application. Detailed Implementation

[0032] Unless otherwise defined, all technical and / or scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. It should be understood that certain features of the invention (described in the context of separate embodiments for clarity) may also be provided in a single embodiment. Conversely, multiple features of the invention (described in the context of a single embodiment for brevity) may also be provided separately or in any suitable combination or, where appropriate, in any other described embodiment of the invention. Certain features described in the context of various embodiments will not be considered essential features of those embodiments unless the embodiment is inoperable without those elements. The invention is further illustrated below by specific examples; however, it should be noted that the specific process conditions and results described in the embodiments of the invention are merely illustrative and should not be construed as limiting the scope of protection of the invention. All equivalent changes or modifications made in accordance with the spirit and essence of the invention should be covered within the scope of protection of the invention.

[0033] This invention provides a flavor-enhancing paper filter rod based on bioelectrofermentation, comprising modified high-permeability forming paper and effective aroma-enhancing components derived from tobacco concentrate fixed thereon through a loading and drying process. The modified high-permeability forming paper is coated with an adsorption enhancer made from a mixture of polyvinyl alcohol and nano-silica. The tobacco concentrate is prepared by bioelectrofermentation of waste tobacco under a weak electric field.

[0034] Furthermore, the basis weight of the modified high-transparency forming paper is 20 g / m³. 2 ~28g / m 2 The air permeability is 8000CU~12000CU, and the tensile strength is 2.5N / mm. 2 ~3.5N / mm 2 Furthermore, the coating amount of the adsorption enhancer is 2 g / m². 2 ~5g / m 2 .

[0035] Furthermore, the aroma-enhancing paper filter rod has a circumference of 16.9mm~24.1mm, a length of 100mm~120mm, a pressure drop of 4000Pa~7500Pa, and a hardness of 80%~90%.

[0036] This invention also provides a method for preparing a flavoring paper filter rod based on bioelectrofermentation, comprising the following steps:

[0037] (1) After pretreatment of waste tobacco, it is mixed with deionized water to obtain a slurry.

[0038] Specifically, the steps are detailed below:

[0039] Tobacco fragments, dust, or tobacco processing waste are selected as raw materials and pulverized at low temperature of -10℃ to 15℃ using an ultra-micro pulverizer. After pulverization, tobacco powder of 80 mesh to 300 mesh is collected by sieving.

[0040] Tobacco powder and deionized water were mixed at a solid-liquid ratio of 1:(5~10) to prepare a slurry.

[0041] (2) Using slurry as fermentation substrate, inoculate with a compound strain and carry out bioelectric fermentation under a weak electric field to obtain fermentation broth; centrifuge, filter, concentrate and purify the fermentation broth to obtain tobacco concentrate.

[0042] Specifically, the steps are detailed below:

[0043] Using slurry as the fermentation substrate, a composite strain of Bacillus subtilis and lactic acid bacteria was inoculated at an inoculation rate of 5% to 10% of the substrate mass. Fermentation was carried out at 30℃ to 35℃ and 70% to 80% humidity. During the fermentation process, a weak electric field of 0.1V / cm to 0.3V / cm was applied through platinum electrodes set in the fermenter. The fermentation time was 7 to 10 days to obtain the fermentation broth. The colony count ratio of Bacillus subtilis to lactic acid bacteria in the composite strain was (1 to 2): 1.

[0044] After fermentation, the fermentation broth is centrifuged and filtered. The supernatant is then passed through an ultrafiltration membrane and a nanofiltration membrane for membrane concentration. The concentration process is controlled at a pressure of 0.1 MPa to 0.3 MPa and a temperature of 30℃ to 40℃ until the solid content is 20% to 30%. The ultrafiltration membrane has a molecular weight cutoff of 1000 Da to 5000 Da, and the nanofiltration membrane has a molecular weight cutoff of 200 Da to 500 Da.

[0045] After concentration, the concentrate is purified by sequentially passing it through an activated carbon chromatography column and an ion exchange resin column to obtain tobacco concentrate; wherein the flow rate of the activated carbon chromatography column is 1 BV / h~2 BV / h, the flow rate of the ion exchange resin column is 2 BV / h~3 BV / h, and the ion exchange resin is a weakly acidic cation exchange resin.

[0046] (3) An adsorption enhancer is coated on the surface of the high-transparency forming paper to obtain modified high-transparency forming paper, wherein the adsorption enhancer is made by mixing polyvinyl alcohol and nano-silica as raw materials.

[0047] Specifically, the steps are detailed below:

[0048] Polyvinyl alcohol (PVA) and nano-silica were mixed at a mass ratio of (3~5):1 to prepare an adsorption reinforcing agent, which was then coated onto the surface of high-transparency forming paper using a doctor blade coating method. The coating amount of the dry adsorption reinforcing agent was 2 g / m². 2 ~5g / m 2After coating, the paper is dried at 60℃~70℃ to obtain modified high-transparency forming paper, with a basis weight of 20 g / m³. 2 ~28g / m 2 The air permeability is 8000CU~12000CU, and the tensile strength is 2.5N / mm. 2 ~3.5N / mm 2 .

[0049] (4) The tobacco concentrate is added to the surface of the modified high-permeability forming paper by spraying or impregnation, and then rolled and cured to prepare the aroma-enhancing paper filter rod.

[0050] Specifically, the steps are detailed below:

[0051] When applying tobacco concentrate to the surface of modified high-transparency forming paper using a spraying process, the spray gun pressure should be controlled at 0.3MPa~0.5MPa, the distance between the spray gun and the modified high-transparency forming paper should be 15cm~20cm, and the amount of tobacco concentrate added should be 10g / m³. 2 ~15g / m 2 The ambient temperature during the addition process is 20℃~25℃, and the relative humidity is 40%~50%.

[0052] When adding tobacco concentrate to the surface of modified high-permeability forming paper using an impregnation process, the modified high-permeability forming paper is immersed in the tobacco concentrate for 3 to 5 minutes, stirred at a low speed of 10 to 20 rpm, and then excess liquid is removed by a squeeze roller with a pressure of 0.1 MPa to 0.2 MPa after impregnation. After impregnation, the basis weight of the modified high-permeability forming paper loaded with tobacco concentrate is 34 g / m³. 2 ~39g / m 2 .

[0053] Modified high-transparency forming paper with added tobacco concentrate is wound around a core rod using a filter rod forming machine at a winding speed of 10,000 to 12,000 pieces / minute and a winding tension of 10N to 15N. After winding, it is cured at 60℃ to 70℃ and a hot air flow rate of 1m / s to 2m / s for 10 to 15 minutes to obtain flavored paper filter rods. The flavored paper filter rods of this application must meet the following indicators after forming: circumference of 16.9mm to 24.1mm, length of 100mm to 120mm, filter rod pressure drop of 4000Pa to 7500Pa, and hardness of 80% to 90%.

[0054] This application uses conventional cigarettes (84 mm in length, 24.3 mm in circumference, and 25 mm in filter length) and slim cigarettes (97 mm in length, 17.0 mm in circumference, and 30 mm in filter length) as a basis, designing filter structures of 10 mm acetate fiber segment + 15 mm paper segment and 15 mm acetate fiber segment + 15 mm paper segment, respectively. Simultaneously, blank filter rod samples were prepared as controls. The same series of filter rod samples were prepared on the same machine, and the aforementioned flavoring paper filter rods and blank filter rods were used to roll the same series of cigarette samples on the same machine.

[0055] The present invention will be described in detail below through specific examples and embodiments. It should also be understood that the following embodiments are only for specific illustration of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-essential improvements and adjustments made by those skilled in the art based on the above description of the present invention are within the scope of protection of the present invention. The specific process parameters, etc., in the following examples are merely examples within a suitable range; that is, those skilled in the art can make appropriate selections within the appropriate range based on the description herein, and are not intended to be limited to the specific values ​​in the examples below.

[0056] Example 1

[0057] (1) Select waste tobacco processing powder as raw material, use an ultra-micro pulverizer to perform low-temperature pulverization at 15℃, and collect 200-mesh tobacco powder by sieving after pulverization; mix tobacco powder with deionized water at a solid-liquid ratio of 1:8 to prepare slurry.

[0058] (2) Using slurry as fermentation substrate, a composite strain of Bacillus subtilis and lactic acid bacteria was inoculated at an inoculation amount of 8% of the fermentation substrate mass. Fermentation was carried out in a constant temperature and humidity fermentation chamber at 32℃ and 75% humidity. During the fermentation process, a constant voltage weak electric field of 0.2V / cm was applied through a platinum electrode set in the fermentation tank. Throughout the fermentation process, the pH of the fermentation broth was stabilized at 6.0~7.0 by adding 0.5mol / L citric acid solution or 0.5mol / L NaOH solution. The stirring speed was maintained at 100r / min. The fermentation time was 8 days to obtain the fermentation broth. Among them, the colony count ratio of Bacillus subtilis to lactic acid bacteria in the composite strain was 1:1.

[0059] After fermentation, the fermentation broth was centrifuged at 3000 r / min for 15 min. The supernatant was then passed through an ultrafiltration membrane and a nanofiltration membrane for membrane concentration. The concentration process was controlled at a pressure of 0.2 MPa and a temperature of 35℃ until the solid content was 25%. The molecular weight cutoff of the ultrafiltration membrane was 3000 Da, and that of the nanofiltration membrane was 300 Da.

[0060] After concentration, the concentrate is purified by sequentially passing it through an activated carbon chromatography column and an ion exchange resin column to obtain tobacco concentrate. The flow rate of the activated carbon chromatography column is 1.5 BV / h, the flow rate of the ion exchange resin column is 2.5 BV / h, the ion exchange resin is a weakly acidic cation exchange resin, and the mass concentration of the concentrate after treatment is approximately 300 g / L.

[0061] (3) Polyvinyl alcohol (PVA) and nano-silica were mixed at a mass ratio of 3:1 to prepare an adsorption reinforcing agent, which was then coated onto the surface of high-transparency forming paper using a doctor blade coating method. The coating amount of the adsorption reinforcing agent was 3 g / m². 2 After coating, the paper is dried at 70℃ to obtain modified high-transparency forming paper, with a basis weight of 24 g / m³. 2 It has an air permeability of 12000 CU and a tensile strength of 3.0 N / mm². 2 .

[0062] (4) The tobacco concentrate was added to the surface of the modified high-transparency forming paper using a spraying process. The spray gun pressure was controlled at 0.4 MPa, the distance between the spray gun and the modified high-transparency forming paper was 18 cm, and the amount of tobacco concentrate added was 12 g / m². 2 The ambient temperature during the addition process was 22℃, and the relative humidity was 45%. The basis weight of the modified high-permeability forming paper loaded with tobacco concentrate was 36 g / m³. 2 .

[0063] Modified high-permeability forming paper with added tobacco concentrate was rolled around a core rod using a PROTOS filter rod forming machine from HAUNI GmbH, Germany. The rolling speed was 11,000 rods / minute and the rolling tension was 12N. After rolling, the filter rod was cured for 12 minutes at 65°C and a hot air flow rate of 1.5m / s to obtain the flavored paper filter rod.

[0064] Example 2

[0065] (1) Select waste tobacco processing fragments as raw materials, use an ultra-micro pulverizer to perform low-temperature pulverization at -5℃, and collect 300-mesh tobacco powder by sieving after pulverization; mix tobacco powder with deionized water at a solid-liquid ratio of 1:5 to prepare slurry.

[0066] (2) Using slurry as fermentation substrate, a composite strain of Bacillus subtilis and lactic acid bacteria was inoculated at an inoculation amount of 5% of the fermentation substrate mass. Fermentation was carried out in a constant temperature and humidity fermentation chamber at 30℃ and 70% humidity. During the fermentation process, a constant voltage weak electric field of 0.1V / cm was applied through a platinum electrode set in the fermentation tank. Throughout the fermentation process, the pH of the fermentation broth was stabilized at 6.0~7.0 by adding 0.5mol / L citric acid solution or 0.5mol / L NaOH solution. The stirring speed was maintained at 80r / min. The fermentation time was 7 days to obtain the fermentation broth. Among them, the colony count ratio of Bacillus subtilis to lactic acid bacteria in the composite strain was 2:1.

[0067] After fermentation, the fermentation broth was centrifuged at 3000 r / min for 15 min. The supernatant was then passed through an ultrafiltration membrane and a nanofiltration membrane for membrane concentration. The concentration process was controlled at a pressure of 0.1 MPa and a temperature of 30℃ until the solid content was 20%. The ultrafiltration membrane had a molecular weight cutoff of 1000 Da and the nanofiltration membrane had a molecular weight cutoff of 200 Da.

[0068] After concentration, the concentrate is purified by sequentially passing it through an activated carbon chromatography column and an ion exchange resin column to obtain tobacco concentrate. The flow rate of the activated carbon chromatography column is 1 BV / h, the flow rate of the ion exchange resin column is 2 BV / h, the ion exchange resin is a weakly acidic cation exchange resin, and the mass concentration of the concentrate after treatment is approximately 250 g / L.

[0069] (3) Polyvinyl alcohol (PVA) and nano-silica were mixed at a mass ratio of 4:1 to prepare an adsorption reinforcing agent, which was then coated onto the surface of high-transparency forming paper using a doctor blade coating method. The coating amount of the adsorption reinforcing agent was 3 g / m². 2 After coating, the paper is dried at 70℃ to obtain modified high-transparency forming paper, with a basis weight of 24 g / m³. 2 It has an air permeability of 12000 CU and a tensile strength of 3.2 N / mm². 2 .

[0070] (4) The tobacco concentrate is added to the surface of the modified high-transparency forming paper using a spraying process. The tobacco concentrate is atomized and sprayed evenly onto the surface of the modified high-transparency forming paper. The spray gun pressure is controlled at 0.3 MPa, the distance between the spray gun and the forming paper is 15 cm, and the amount of tobacco concentrate added is 10 g / m². 2 The spraying process was controlled at an ambient temperature of 20℃ and a relative humidity of 40%. After spraying, the paper was dried at 60℃ with a hot air flow rate of 1m / s. After drying, the basis weight of the modified high-permeability forming paper loaded with tobacco concentrate was 34g / m². 2 .

[0071] Modified high-permeability forming paper with added tobacco concentrate was rolled around a core rod using a PROTOS filter rod forming machine from HAUNI GmbH, Germany. The rolling speed was 10,000 rods / minute and the rolling tension was 10N. After rolling, the filter rod was cured for 10 minutes at 60℃ and a hot air flow rate of 1m / s to obtain the flavored paper filter rod.

[0072] Example 3

[0073] (1) Select waste tobacco processing powder as raw material, use an ultra-micro pulverizer to perform low-temperature pulverization at 0℃, and collect 80-mesh tobacco powder by sieving after pulverization; mix tobacco powder with deionized water at a solid-liquid ratio of 1:10 to prepare slurry.

[0074] (2) Using slurry as fermentation substrate, a composite strain of Bacillus subtilis and lactic acid bacteria was inoculated at an inoculation amount of 10% of the fermentation substrate mass. Fermentation was carried out in a constant temperature and humidity fermentation chamber at 35℃ and 80% humidity. During the fermentation process, a constant voltage weak electric field of 0.3V / cm was applied through a platinum electrode set in the fermentation tank. Throughout the fermentation process, the pH of the fermentation broth was stabilized at 6.0~7.0 by adding 0.5mol / L citric acid solution or 0.5mol / L NaOH solution. The stirring speed was maintained at 50r / min. The fermentation time was 10 days to obtain the fermentation broth. Among them, the colony count ratio of Bacillus subtilis to lactic acid bacteria in the composite strain was 1:1.

[0075] After fermentation, the fermentation broth was centrifuged at 3000 r / min for 15 min. The supernatant was then passed through an ultrafiltration membrane and a nanofiltration membrane for membrane concentration. The concentration process was controlled at a pressure of 0.3 MPa and a temperature of 40℃ until the solid content was 30%. The ultrafiltration membrane had a molecular weight cutoff of 5000 Da and the nanofiltration membrane had a molecular weight cutoff of 500 Da.

[0076] After concentration, the concentrate is purified by sequentially passing it through an activated carbon chromatography column and an ion exchange resin column to obtain tobacco concentrate. The flow rate of the activated carbon chromatography column is 2 BV / h, the flow rate of the ion exchange resin column is 3 BV / h, the ion exchange resin is a weakly acidic cation exchange resin, and the mass concentration of the concentrate after treatment is approximately 350 g / L.

[0077] (3) Polyvinyl alcohol (PVA) and nano-silica were mixed at a mass ratio of 3:1 to prepare an adsorption reinforcing agent, which was then coated onto the surface of high-transparency forming paper using a doctor blade coating method. The coating amount of the adsorption reinforcing agent was 3 g / m². 2 After coating, the paper is dried at 70℃ to obtain modified high-transparency forming paper, with a basis weight of 24 g / m³. 2 It has an air permeability of 12000 CU and a tensile strength of 3.0 N / mm². 2 .

[0078] (4) The tobacco concentrate is added to the surface of the modified high-transparency forming paper using a spraying process. The tobacco concentrate is atomized and sprayed evenly onto the surface of the modified high-transparency forming paper. The spray gun pressure is controlled at 0.5 MPa, the distance between the spray gun and the forming paper is 20 cm, and the amount of tobacco concentrate added is 15 g / m². 2 The spraying process was controlled at an ambient temperature of 25℃ and a relative humidity of 50%. After spraying, the paper was dried at 60℃ and a hot air flow rate of 1m / s for 5 minutes. After drying, the basis weight of the modified high-permeability forming paper loaded with tobacco concentrate was 39g / m².

[0079] Modified high-permeability forming paper with added tobacco concentrate was rolled around a core rod using a PROTOS filter rod forming machine from HAUNI GmbH, Germany. The rolling speed was 12,000 rods / minute and the rolling tension was 15N. After rolling, the filter rod was cured at 70℃ and hot air flow rate of 2m / s for 15 minutes to obtain the flavored paper filter rod.

[0080] Comparative Example 1

[0081] The difference between this comparative example and Example 1 is as follows:

[0082] (2) Using slurry as fermentation substrate, a compound strain of Bacillus subtilis and lactic acid bacteria was inoculated at an inoculation amount of 8% of the fermentation substrate mass. The fermentation was carried out in a constant temperature and humidity fermentation chamber at 32℃ and 75% humidity for 8 days to obtain fermentation liquid. The colony count ratio of Bacillus subtilis to lactic acid bacteria in the compound strain was 1:1.

[0083] Comparative Example 2

[0084] The difference between this comparative example and Example 1 is as follows:

[0085] Using slurry as the fermentation substrate, a composite strain of Bacillus subtilis and lactic acid bacteria was inoculated at an inoculation amount of 8% of the substrate mass. Fermentation was carried out in a constant temperature and humidity fermentation chamber at 32℃ and 75% humidity. During the fermentation process, an electric field of 1V / cm was applied through a platinum electrode set in the fermentation tank. The fermentation time was 8 days to obtain the fermentation broth. The colony count ratio of Bacillus subtilis to lactic acid bacteria in the composite strain was 1:1.

[0086] Comparative Example 3

[0087] The difference between this comparative example and Example 1 is as follows:

[0088] Without modifying the high-transparency forming paper, tobacco concentrate is directly added to the surface of the high-transparency forming paper.

[0089] Comparative Example 4

[0090] The difference between this comparative example and Example 1 is as follows:

[0091] (3) The adsorption enhancer is cationic starch.

[0092] This application uses conventional cigarettes (84 mm in length, 24.3 mm in circumference, and 25 mm in filter length) and slim cigarettes (97 mm in length, 17.0 mm in circumference, and 30 mm in filter length) as a basis, and designs filter structures of 10 mm acetate fiber segment + 15 mm paper segment and 15 mm acetate fiber segment + 15 mm paper segment, respectively. Simultaneously, blank filter rod samples are prepared as controls. The same series of filter rod samples are prepared on the same machine using the flavor-enhancing paper filter rods and blank filter rods prepared in Examples 1-3 and Comparative Examples 1-4, and the same type of tobacco to roll the same series of cigarette samples on the same machine.

[0093] Performance testing

[0094] Detection of flavor compounds in fermentation concentrate: Gas chromatography-mass spectrometry (GC-MS) was used to detect the content of aroma-producing compounds (terpenes, phenols, ketones, unit: mg / L) and off-gas compounds (hydrogen sulfide, amines, unit: mg / L) in the tobacco fermentation concentrate of each sample. The data are the average values ​​of three parallel experiments. The results are shown in Table 1.

[0095] Table 1. Detection results (mg / L) of flavor compounds in the concentrated tobacco fermentation liquid of the examples and comparative examples.

[0096]

[0097] The flavor compound content of tobacco fermentation concentrate was detected by GC-MS (according to YC / T159-2021). The results showed that after fermentation with a composite strain under a weak electric field of 0.1~0.3V / cm in Examples 1-3, the total aroma compound content reached 300.4mg / L, 282.4mg / L, and 309.7mg / L, respectively, while the total content of off-gas compounds was only 0.23mg / L, 0.25mg / L, and 0.21mg / L. In contrast, the total aroma compound content of Comparative Example 1 without an electric field was 169.0mg / L, and the off-gas compound content reached 0.93mg / L. The total aroma compound content of Comparative Example 2 with a strong electric field of 1V / cm was as low as 131.0mg / L, and the off-gas compound content reached 1.07mg / L. Comparative Examples 3 and 4, because they were fermented with the same strain as Example 1, showed lower levels of aroma compounds. The fermentation process showed that the content of aroma-producing and off-gas substances was highly consistent with that in Example 1, at 298.8 mg / L, 299.6 mg / L and 0.23 mg / L, 0.21 mg / L, respectively. This result is due to the fact that a weak electric field of 0.1~0.3 V / cm can precisely activate the aroma-producing metabolic pathway of Bacillus subtilis and lactic acid bacteria and effectively inhibit the generation of off-gas substances. Without an electric field, the aroma-producing metabolism of the strains cannot be activated, resulting in a significant reduction in aroma-producing efficiency. A strong electric field of 1 V / cm will directly damage the microbial cell membrane, causing the strain's metabolism to stagnate and its aroma-producing ability to be completely impaired. It will also release intracellular impurities due to cell membrane rupture, further increasing off-gas substances. In contrast, Comparative Examples 3 and 4 only changed the subsequent paper modification and adsorbent process, without involving the fermentation process. Therefore, the content of flavor substances in the concentrate did not change significantly.

[0098] Results of finished cigarette draw resistance and routine smoke emission tests: The finished cigarettes prepared in the above examples and comparative examples were first equilibrated in a standard environment of 22±1℃ and 60±2% relative humidity for 48 hours, and then draw resistance and routine smoke emission were tested. The draw resistance of the cigarettes was tested according to GB / T22839-2021, and the tar, nicotine and CO were tested according to GB / T 19609-2021, YC / T 160-2021, GB / T 23356-2021 and other standards. Each group of samples was tested 3 times and the average value was taken. At the same time, blank cigarettes were used as a control group. The results are shown in Table 2.

[0099] Table 2. Results of draw resistance and routine smoke measurement for each finished cigarette product.

[0100]

[0101] As shown in Table 2, the finished cigarettes made by rolling the aroma-enhancing paper filter rods and tobacco shreds prepared in the embodiments of this application have a stable draw resistance of 1050±200Pa for conventional cigarettes and 900±200Pa for slim cigarettes. The draw resistance values ​​of each embodiment are all within the standard range and are consistent with the blank group, and all meet the relevant standard requirements. The aroma-enhancing paper filter rods from Examples 1-3 were rolled with tobacco (designed tar content of 5mg for conventional cigarettes and 3mg for slim cigarettes) to form finished cigarettes. Tested according to relevant standards, the conventional cigarettes contained 4.8-5.0mg of tar, 0.47-0.48mg of nicotine, and 5.0-5.1mg of carbon monoxide; the slim cigarettes contained 3.1-3.3mg of tar, 0.31-0.33mg of nicotine, and 3.4-3.6mg of carbon monoxide, all meeting the design requirements. Compared with the finished cigarettes equipped with the control group filter rods, the deviations in tar and nicotine content were ≤0.3mg, achieving the technical goal of "reducing tar without reducing aroma."

[0102] Comparative Examples 1 and 2 showed similar draw resistance to the Example but slightly higher smoke indicators. This was due to abnormal fermentation processes (no weak electric field / strong electric field applied), which only affected the quality of the tobacco concentrate without altering the physical structure of the filter rod. The abnormal fermentation resulted in insufficient effective aroma-retaining components in the concentrate, and the presence of undegraded macromolecular impurities and irritating byproducts. When smoke passed through the filter rod, it could not rely on the high-quality concentrate and adsorption enhancer to achieve efficient synergistic retention, leading to the slight escape of harmful substances such as tar, nicotine, and CO. Comparative Example 3 had significantly lower draw resistance (986 Pa for conventional, 852 Pa for slim) and the highest smoke indicators among all groups. This was because the forming paper was unmodified, lacking the pore optimization and aroma-fixing adsorption effect of the adsorption enhancer. Excessive porosity reduced draw resistance, but also prevented... Effectively trapping harmful substances, but the concentrate is easily lost, and impurities in the base paper are also released; Comparative Example 4 shows a slight decrease in draw resistance (1025 Pa for conventional, 890 Pa for slim cigarettes), but higher smoke indicators. This is because the single cationic starch replaces the compound adsorption enhancer, resulting in insufficient film formation and adsorption performance, leading to a loose paper structure, a slight decrease in draw resistance, and a significant reduction in the ability to fix aroma substances and trap harmful substances; The blank cellulose acetate filter group has the highest draw resistance (1184 Pa for conventional, 967 Pa for slim cigarettes) and the lowest smoke indicators. This is because the dense fiber bundle structure of the cellulose acetate filter itself creates higher resistance to smoke passage. At the same time, it does not introduce additional components of tobacco concentrate, relying only on the physical trapping effect of cellulose acetate itself, so the release of tar, nicotine, and CO in the smoke is the lowest.

[0103] Sensory evaluation: Referring to the methods of GB / T 5606.4—2005 and YC / T 497—2014, a professional evaluation team of 7 people conducted sensory evaluations on the finished cigarettes prepared in the above embodiments and comparative examples using a group comparative evaluation method. The full score for each evaluation index (aroma, off-flavors, harmony, irritation, and aftertaste) was 10 points. The arithmetic mean of the scores of the 7 people was taken as the final result. The results are shown in Table 3.

[0104] Table 3 Sensory evaluation results of each finished cigarette product

[0105]

[0106] As shown in Table 3, the finished cigarettes prepared in each embodiment of this application are significantly better than the comparative example and the blank control group in all sensory dimensions. Compared with the corresponding specification blank group, the aroma is improved by 23%~29%, the control effect of impurities is improved by 4%~13%, the harmony is improved by 32%~37%, the irritation is improved by 15%~26%, and the aftertaste is improved by 16%~23%. This fully verifies that the filter rod of this invention can effectively solve the industry pain points of "light aroma and weak strength" and prominent impurities and irritation in ultra-low tar cigarettes.

[0107] Comparative Example 1 had the lowest total score (38.6 for regular cigarettes and 37.4 for slim cigarettes), lagging significantly in all dimensions. This was because a weak electric field was not applied during fermentation, the microbial aroma-producing pathways were not activated, macromolecular degradation was insufficient, the concentrate had insufficient aroma and many impurities, resulting in a weak cigarette aroma, poor harmony, and a significant increase in off-flavors and irritation. Comparative Example 2 had a slightly lower total score (41.4 for regular cigarettes and 39.6 for slim cigarettes) and a higher irritation score. This was because a strong electric field was applied during fermentation, which damaged the microbial structure, inhibiting the synthesis of aroma substances and producing a small amount of irritating byproducts, affecting the cigarette smoking experience. Comparative Examples 3 and 4 had similar total scores (40.3-40.5 for regular cigarettes and 38 for slim cigarettes). (7~38.8 points) and the aroma and harmony dimensions were poor. Among them, Comparative Example 3 was due to the unmodified forming paper, which lacked the aroma-fixing and slow-release effect of the adsorption enhancer. The aroma substances were easily lost and the impurities of the original paper were easily released. Comparative Example 4 was due to the use of cationic starch instead of compound adsorption enhancer, which resulted in insufficient film-forming adsorption performance, weak fixation of aroma substances and uneven release. It also increased the off-flavor of cigarettes due to its own impurities. The blank cellulose acetate filter rod scored the lowest in all dimensions, with a total score of only about 34 points for both regular and slim cigarettes. Because it only has the basic smoke filtration function and no aroma-enhancing / strength-enhancing ingredients, the cigarette smoke itself has a weak aroma and insufficient strength, which became the basic control benchmark for this sensory quality evaluation.

[0108] The above embodiments are merely illustrative of the principles and effects of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.

Claims

1. A flavor-enhancing paper filter rod based on bioelectrofermentation, characterized in that, The aroma-enhancing paper filter rod includes modified high-permeability forming paper and effective aroma-enhancing components derived from tobacco concentrate fixed thereon through a loading and drying process. The surface of the modified high-permeability forming paper is coated with an adsorption enhancer made from a mixture of polyvinyl alcohol and nano-silica. The tobacco concentrate is prepared from waste tobacco through bio-electro-fermentation under a weak electric field.

2. The aroma-enhancing paper filter rod according to claim 1, characterized in that, The basis weight of the modified high-transparency forming paper is 20 g / m³. 2 ~28g / m 2 The air permeability is 8000CU~12000CU, and the tensile strength is 2.5N / mm. 2 ~3.5N / mm 2 The coating amount of the adsorption enhancer is 2 g / m³. 2 ~5g / m 2 .

3. The aroma-enhancing paper filter rod according to claim 1, characterized in that, The aroma-enhancing paper filter rod has a circumference of 16.9mm~24.1mm, a length of 100mm~120mm, a pressure drop of 4000Pa~7500Pa, and a hardness of 80%~90%.

4. A method for preparing a flavoring paper filter rod based on bioelectrofermentation, characterized in that, Includes the following steps: (1) After pretreatment of waste tobacco, it is mixed with deionized water to prepare a slurry; (2) Using the slurry as a fermentation substrate, a compound bacterial strain is inoculated and bioelectric fermentation is carried out under a weak electric field to obtain a fermentation broth; the fermentation broth is centrifuged, filtered, concentrated and purified to obtain a tobacco concentrate. (3) An adsorption enhancer is coated on the surface of the high-transparency forming paper to obtain modified high-transparency forming paper, wherein the adsorption enhancer is made by mixing polyvinyl alcohol and nano-silica as raw materials; (4) The tobacco concentrate is added to the surface of the modified high-transparency forming paper by spraying or impregnation, and then rolled and cured to prepare the aroma-enhancing paper filter rod.

5. The preparation method according to claim 4, characterized in that, In step (1), the waste tobacco is pretreated to obtain tobacco powder, and the solid-liquid ratio of the tobacco powder to the deionized water is 1:(5~10).

6. The preparation method according to claim 4, characterized in that, The fermentation process of the fermentation broth in step (2) satisfies at least one of the following conditions (a) to (d): (a) The composite strain is Bacillus subtilis and lactic acid bacteria, and the colony count ratio of Bacillus subtilis to lactic acid bacteria is (1~2):1; (b) The inoculum size of the compound strain is 5% to 10% of the fermentation substrate mass; (c) The weak electric field is 0.1V / cm to 0.3V / cm; (d) The fermentation time is 7 to 10 days.

7. The preparation method according to claim 6, characterized in that, The preparation process of tobacco concentrate in step (2) satisfies at least one of the following conditions (e) to (f): (e) The concentration is a membrane concentration by sequentially passing the material through an ultrafiltration membrane and a nanofiltration membrane, and the concentration is carried out until the solid content is 20% to 30%; (f) The purification process includes sequentially passing the material through an activated carbon chromatography column and an ion exchange resin column.

8. The preparation method according to claim 4, characterized in that, In step (3), the mass ratio of polyvinyl alcohol to nano-silica is (3~5):

1.

9. The preparation method according to claim 4, characterized in that, In step (4), when a spraying process is used, the amount of tobacco concentrate added is 10 g / m³. 2 ~15g / m 2 .

10. The preparation method according to claim 4, characterized in that, In step (4), when the impregnation process is used, the basis weight of the modified high-permeability forming paper loaded with the tobacco concentrate after impregnation is 34 g / m³. 2 ~39g / m 2 .

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