Anti-coagulant protein triabin containing tyrosine sulfation modification and semi-chemical synthesis method thereof

Abstract

The application provides an anti-coagulation protein Triabin containing a tyrosine sulfation modification and a semi-chemical synthesis method thereof. According to the distance, the Triabin is divided into fragment 1 and fragment 2 according to the closest cysteine to a sulfated tyrosine site, the sulfated tyrosine site is located in the fragment 1, and the sulfated tyrosine site is close to the C-terminal of the fragment 1; the polypeptide solid-phase synthesis technology is used to synthesize a fragment 1 polypeptide resin, and after cleavage, deprotection, extraction and purification, the fragment 1 with an amide C-terminal is obtained; the E. coli recombinant expression technology is used to obtain the fragment 2 with a hydrazine C-terminal; the thioester of the fragment 2 with the hydrazine C-terminal is converted, the natural chemical linking reaction and the neopentyl removal reaction are used to obtain a full-length linear protein, and the anti-coagulation protein Triabin containing the tyrosine sulfation modification is obtained through a refolding reaction. The anti-coagulation protein Triabin containing the tyrosine sulfation modification has stronger anti-coagulation activity than the wild-type Triabin.

Images