A multiplex real-time fluorescent PCR reaction system based on probe differentiation design and a high-throughput PCR result analysis system
By combining probe differentiation design with a neural network model, the problem of limited fluorescence channels in multiplex fluorescent PCR was solved, enabling accurate detection of multiple targets in a single fluorescence channel, thus improving detection throughput and accuracy.
CN122128415APending Publication Date: 2026-06-02SUZHOU HAIMIAO BIOTECH CO LTD
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SUZHOU HAIMIAO BIOTECH CO LTD
- Filing Date
- 2026-01-08
- Publication Date
- 2026-06-02
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Figure CN122128415A_ABST
Abstract
The application provides a multiplex real-time fluorescent PCR reaction system based on probe differentiation design, corresponding fluorescent probes are designed based on target genes, the fluorescent probes are labeled with 1-3 fluorescent groups and 1-3 quenching groups, and the number and / or labeling position of the fluorescent groups and / or quenching groups carried by the fluorescent probes of different target genes are different. The reaction system can further carry multi-dimensional data analysis and a neural network model based on experience learning, unknown sample target gene sample types can be quickly and accurately judged, and accurate differentiation of multiple target genes in a single fluorescent channel can be realized.
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Citation Information
Patent Citations
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