96-channel polypeptide synthesizer and synthesis method

Abstract

This invention discloses a 96-channel peptide synthesizer and its synthesis method, comprising a reaction system, a solvent management system, and a control system. The reaction system includes a housing, a reaction rack, reactors, a dispensing tower, and amino acid bottles. The housing is equipped with partitions to achieve functional zoning. The reaction rack houses 96 detachable reactors with oscillating stirring capabilities. The amino acid bottles feature quick-release pull-rings and cylinder-operated metering structures. The dispensing tower employs staggered operation for synchronous liquid supply. The solvent management system uses positive nitrogen pressure as its power source and achieves precise solvent delivery through a dispensing pump, transfer bottle, and solvent valve. The control system is fully automated via computer control, capable of monitoring channel status, editing process parameters, and supporting mode switching. This invention achieves high-throughput, automated peptide synthesis with 96 channels, offering advantages such as convenient operation and high synthesis efficiency.

Description

Technical Field

[0001] This invention belongs to the field of chemical synthesis technology, and in particular relates to a 96-channel peptide synthesizer and synthesis method. Background Technology

[0002] Peptide synthesizers are core equipment for peptide drug development and peptide material preparation. Traditional peptide synthesizers are mostly single-channel or low-channel models, which have problems such as low synthesis efficiency, poor batch consistency, high solvent consumption, and low degree of automation, making it difficult to meet the needs of high-throughput peptide synthesis.

[0003] Existing 96-channel synthesis equipment generally suffers from defects such as inaccurate liquid addition, incomplete drainage, difficult-to-clean reactor blockage, and unstable gas pressure control. Furthermore, the operation process is complex, affecting the purity and yield of peptide synthesis. Therefore, developing a highly automated, precise liquid addition, smooth drainage, safe, stable, and GMP-compliant 96-channel peptide synthesizer and its supporting synthesis method has significant application value. Summary of the Invention

[0004] To address the above problems, the present invention provides a 96-channel peptide synthesizer and a synthesis method.

[0005] To achieve the above objectives, the present invention provides a 96-channel peptide synthesizer, comprising a reaction system, a solvent management system, and a control system. The reaction system includes a housing, a reaction rack, a reactor, a liquid addition tower, and an amino acid bottle. A partition is fixedly installed inside the housing, and a hollow space above the partition is used to install the liquid addition tower and the amino acid bottle. The reaction rack is fixedly installed on the partition. The reaction rack uses an upper lid for liquid addition and a lower lid for liquid discharge. The reactor can be oscillated and stirred 360°.

[0006] Optionally, the reactor has a detachable structure, with a handle integrally formed on the outside of the reactor, a filter plate embedded inside the reactor, a solvent inlet at the top of the reactor, and a drain outlet at the bottom.

[0007] Optionally, the amino acid bottle is arranged above the reactor, and the bottom is provided with a pull-ring quick-release structure and a cylinder tapping metering mechanism.

[0008] Optionally, the liquid addition tower is fixedly installed in the hollow area above the partition plate, and there are multiple sets of the liquid addition towers. The multiple sets of liquid addition towers are operated in a staggered manner. Each set of liquid addition towers corresponds to multiple reactors and can be connected to multiple solvent and nitrogen pipelines at the same time to realize multi-channel synchronous and precise liquid addition and pipeline residual solvent purging.

[0009] Optionally, the solvent management system includes a dispensing pump, solvent valves, and a transfer bottle. The transfer bottle is fixedly installed on the partition of the housing and is used to transfer and store solvent and stabilize the supply pressure. The dispensing pump connects the transfer bottle and the dispensing tower and is used to accurately deliver the solvent in the transfer bottle to the reactor. The solvent valves are centrally arranged below the partition on the front side of the housing and are used to control the on / off state and flow rate switching of each solvent.

[0010] Optionally, the addition and discharge of liquid in the synthesizer are both driven by positive pressure of nitrogen. The bottom of the housing is provided with a gas / liquid interface and is connected to the reactor for connecting nitrogen, compressed air and solvent pipelines.

[0011] Optionally, the control system adopts a fully automatic computer control mode, which can monitor the operating status of 96 channels in real time, edit peptide synthesis process parameters, manage user permissions and backup synthesis data, and supports switching between automatic operation mode and password-protected manual operation mode.

[0012] Optionally, a rotary button is rotatably connected to the top of the housing for manually adjusting the alignment angle between the reaction rack station and the liquid addition tower, and a valve and a transparent protective cover are provided on the front side of the housing.

[0013] Optionally, the transfer bottle is equipped with a photoelectric sensor on the outside for real-time monitoring of solvent level and prevention of overflow, and the solvent valve is made of corrosion-resistant material and is adapted to control the on / off of various solvents required for peptide synthesis.

[0014] A 96-channel peptide synthesis method includes the following steps: installing a reactor, fixing an amino acid bottle, connecting the solvent and gas pipeline and adjusting the pressure; entering synthesis parameters and editing the synthesis process in the control system; the control system links the reaction system and the solvent management system to automatically complete the cycles of adding liquid, shaking and stirring, draining liquid, and nitrogen purging until the peptide chain synthesis is completed; disassembling the reactor to collect the peptide resin and cleaning the reactor and pipeline.

[0015] The beneficial effects of this invention are as follows: 1. The synthesizer of the present invention significantly improves the high-throughput synthesis efficiency. Through the staggered operation design of multiple liquid addition towers and the layout of 96 reactor arrays, it realizes multi-channel synchronous liquid addition, reaction and liquid discharge, which greatly shortens the waiting time for single batch peptide synthesis. Compared with traditional synthesis equipment, it effectively improves the high-throughput synthesis capability and is suitable for large-scale synthesis needs such as peptide library construction.

[0016] 2. The synthesizer of the present invention has high synthesis accuracy and stability. It relies on the cylinder tapping amino acid metering structure and the precise liquid supply and drainage system driven by nitrogen positive pressure. Combined with the 360° oscillation and stirring design of the reactor, it ensures that the reaction system is mixed evenly and the raw material ratio is accurate, reducing raw material residue and reaction error. At the same time, the design of detachable reactor and filter plate for overall replacement ensures long-term stable operation of the equipment and improves batch consistency of peptide synthesis.

[0017] 3. The synthesizer of this invention features outstanding ease of operation and safety. The control system adopts a fully automatic control mode, which can monitor the channel status in real time, edit process parameters, and support password-protected manual mode switching, reducing the intensity of manual operation. The quick-release structure of the amino acid bottle pull ring and the reactor handle design simplify the disassembly and maintenance process. The transparent protective cover of the box and the pressure control design effectively avoid liquid splashing and equipment damage, improving operational safety and convenience. Attached Figure Description

[0018] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments.

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention.

[0020] Figure 2 This is a schematic diagram to show part of the structure of the reaction system and solvent management system.

[0021] Figure 3 This is a schematic diagram to show part of the structure of the solvent valve.

[0022] Figure 4 This is a schematic diagram of the reactor structure.

[0023] Figure 5 This is a schematic diagram of the structure of an amino acid bottle.

[0024] Figure 6 This is a diagram of the synthesizer piping.

[0025] Figure 7 This is a screenshot of the synthesizer's main interface.

[0026] Explanation of reference numerals in the attached figures 1. Reaction system; 11. Housing; 111. Baffle; 112. Protective cover; 113. Rotary button; 114. Gas / liquid interface; 12. Reaction rack; 13. Reactor; 131. Solvent inlet; 132. Drain; 133. Filter plate; 134. Handle; 14. Addition tower; 15. Amino acid bottle; 151. Pull ring; 2. Solvent management system; 21. Solvent valve; 22. Transfer bottle. Detailed Implementation

[0027] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention may also be implemented in other ways different from those described herein. Those skilled in the art can make similar extensions without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0028] Reference Figure 1 A 96-channel peptide synthesizer mainly consists of three parts: a reaction system 1, a solvent management system 2, and a control system (not shown in the figure). The reaction system 1 is the core functional unit, used to realize the reaction synthesis of peptide resin with various reagents and solvents; the solvent management system 2 is responsible for the precise dispensing of various solvents and activating reagents and the collection of waste liquid; the control system is used for the automatic control of the whole machine, coordinating the operation of each system to realize the automated operation of the entire peptide synthesis process.

[0029] Reference Figure 1-5 The reaction system 1 includes a housing 11, a reaction rack 12, a reactor 13, a liquid addition tower 14, and an amino acid bottle 15. These components work together to complete the reaction, liquid addition, and liquid discharge operations of the peptide resin. The housing 11 is the main load-bearing structure of the reaction system 1. An internal partition 111 is fixedly installed, dividing the interior of the housing 11 into upper and lower functional areas: the upper part of the partition 111 is hollow and hinged with a transparent protective cover 112, forming an upper operating space for installing the liquid addition tower 14, the amino acid bottle 15, and the transfer bottle 22, facilitating the supply and delivery of solvents and raw materials; the lower part of the partition 111 is the lower reaction support area, used to fix the reaction rack 12 and equipped with drainage and gas-liquid communication pipelines. A rotary button 113 is rotatably connected to the top of the housing 11, which can manually adjust the position of the reaction rack 12 or the alignment angle of the liquid addition tower 14; a gas / liquid interface 114 is opened on one side of the bottom of the housing 11. This interface is connected to the reactor 13 and is used to connect to nitrogen, compressed air and solvent delivery pipelines to provide power and medium support for liquid addition, liquid discharge and purging.

[0030] Reference Figure 1-5The reaction rack 12 is fixedly mounted on the partition plate 111 and vertically arranged along the height of the box 11. It is the core supporting structure for the peptide resin reaction. The whole adopts a double-end operation structure with the upper lid for adding liquid and the lower lid for draining liquid, which is suitable for the process requirement of "adding liquid for reaction first and then draining liquid for cleaning" in peptide synthesis. The reaction rack 12 mainly includes reactors 13 and a stirring system. 96 reactors 13 are evenly arranged in an array on the reaction rack 12. The reactors 13 are made of 7mL single-layer PP material and are internally embedded with PE sand core filter plates 133 to achieve solid-liquid separation. The stirring system is linked with the reaction rack 12 and can drive the reactors 13 to oscillate and stir about 360°, ensuring that the peptide resin, amino acid solution and solvent in the reactors 13 are fully mixed, eliminating reaction dead zones and improving synthesis uniformity and efficiency. The upper and lower lids of the reaction vessel 12 are respectively adapted to the liquid addition and drainage system of the box 11. The upper lid connects to the liquid addition tower 14 and the amino acid bottle 15 to receive solvent and amino acid raw materials; the lower lid connects to the valve below the partition 111 and the transfer bottle 22 to complete the discharge and temporary storage of reaction waste liquid.

[0031] Reference Figure 3 The reactor 13 has a solvent inlet 131 at the top, serving as a channel for adding solvent and amino acid solution; and a drain outlet 132 at the bottom, which, together with the lower cover, allows for waste liquid discharge. The lower cover of the reactor 13 is equipped with a PE sand core filter plate 133, which can effectively trap peptide resin in the reaction system while ensuring smooth waste liquid discharge. A handle 134 is integrally formed on the outside of the reactor 13. During disassembly, the operator can simply grasp the handle 134 and pull outwards with a small force to remove the reactor 13 from the reaction frame 12, making maintenance convenient. If the liquid addition or discharge rate slows down during use, indicating that the filter plate 133 is clogged, the entire reactor 13 can be replaced without disassembly. However, depending on the usage requirements, if the filter plate 133 of the plastic reactor 13 is clogged, the entire reactor 13 must be replaced to ensure the stable operation of the reaction system 1. After the reaction and pyrolysis are completed, the reactor 13 can be inverted to pour out the internal peptide resin through the solvent inlet 131, making the operation simple and efficient.

[0032] Reference Figure 2-5The addition towers 14 are fixed in the hollow installation space above the partition plate 111. A total of 12 addition towers 14 are provided, each corresponding to 8 reactors 13, forming a 96-channel synchronous addition layout. Each addition tower 14 can simultaneously connect to 4 types of solvent pipelines and nitrogen pipelines. During addition, the reaction rack 12 drives the reactor 13 to rotate below the corresponding addition tower 14. The addition tower 14 moves downward and opens its valve, using positive nitrogen pressure to precisely deliver the solvent into the reactor 13. After addition is complete, nitrogen continues to purge, completely blowing any residual solvent from the pipelines and addition towers 14 into the reactor 13, avoiding synthesis errors caused by raw material residue. The 12 addition towers 14 employ a staggered operation design, allowing for simultaneous multi-station addition operations, significantly shortening the addition waiting time for a single batch synthesis and adapting to the needs of high-throughput peptide synthesis.

[0033] Reference Figure 1 and Figure 4 Amino acid bottles 15 are installed in the mounting space above the partition 111, corresponding to the arrangement above the reactor 13. A total of 24 single-layer glass bottles of 170mL each are provided for batch storage of amino acid solutions. Each amino acid bottle is equipped with a pull ring 151 on its flat bottom and is also equipped with a cylinder tapping metering structure. Each tap can accurately measure about 2.5mL of amino acid liquid. The system automatically controls the number of taps according to the target liquid addition volume to achieve high-precision supply of amino acid raw materials. The amino acid bottles 15 work in conjunction with the liquid addition tower 14. Through the precise positioning and delivery of the liquid addition tower 14, the amino acid solution is smoothly injected into the reactor 13. The disassembly and installation of the amino acid bottle 15 are convenient: When disassembling, push the liquid addition tower 14 inward by hand, pull the pull ring 151 located at the bottom of the amino acid bottle 15 with your left hand and pull it outward, and hold the amino acid bottle 15 with your right hand and lift it upward to complete the disassembly; when installing, first pull the pull ring 151 outward, put the bottom of the amino acid bottle 15 into the corresponding installation position, release the pull ring 151 to complete the initial fixation, and then pull the liquid addition tower 14 outward to the corresponding position to lock it, thus completing the installation and fixation of the amino acid bottle 15. The disassembly and assembly are highly efficient.

[0034] Reference Figure 1-6The solvent management system 2 is installed on the reaction rack 12 and works closely with the reaction system 1 to complete the entire process of solvent delivery, metering, addition, and discharge. The solvent management system 2 mainly consists of a dispensing pump, solvent valve 21, transfer bottle 22, and supporting pipelines, with a clear layout and compact connections. The transfer bottle 22 is fixedly installed inside the housing 11 at the top, above the reactor 13, for pre-transferring and storing solvent, stabilizing the supply pressure, and is equipped with an external photoelectric sensor to monitor the liquid level and prevent overflow, ensuring safe and reliable dispensing. The dispensing pump is fixed on the pipeline between the transfer bottle 22 and the dispensing tower 14, directly connected to the outlet of the transfer bottle 22, responsible for accurately metering and delivering the solvent in the transfer bottle 22 to the reactor 13, achieving stable liquid supply with the help of positive nitrogen pressure. The solvent valve 21 is centrally located on the front side of the housing 11, in the operating area below the partition 111, forming a connecting pipeline with the transfer bottle 22 and the dispensing pump, used to control the on / off state and flow switching of each solvent path. The valve body is made of corrosion-resistant material to ensure long-term stable operation.

[0035] Reference Figure 1-6 The liquid addition process is powered by positive nitrogen pressure. The liquid addition pump and liquid addition tower 14 work together to complete the metering, which can be precisely controlled in two modes: milliliters or time. The liquid discharge is also driven by positive nitrogen pressure, and multiple channels can discharge simultaneously, which is fast and leaves no residue. The entire solvent management system 2 is equipped with a complete pipeline and gas source access process. Pipeline connections must be strictly in accordance with... Figure 6 Please ensure that all inlet, outlet, and gas balance ports for DMF, PIPE, DCM, condensing agent, positive pressure nitrogen vent, and Waste are connected to the interfaces labeled on the side of the equipment to avoid incorrect connections. When connecting gas sources, the nitrogen inlet pressure must be controlled below 15 psi, and ultimately adjusted to 5 psi; the compressed air inlet pressure must be controlled below 150 psi, and ultimately adjusted to 60 psi. After connection, allow the system to stand and observe for leaks. For external leaks, reconnect the pipeline; for internal leaks, contact after-sales service. After confirming that all switches are in the off position, connect all communication cables to the control cabinet, and finally connect the power cord.

[0036] Strict adherence to operating procedures is required during use: All glass containers must be used within the product's acceptable operating pressure. Handle with care during use or replacement to avoid impact and breakage. Compressed air and nitrogen pressures must be controlled within limits to prevent excessive pressure from causing breakage of glass containers such as reactor 13 and amino acid bottle 15. The liquid addition tower 14 must be moved above amino acid bottle 15 to ensure accurate alignment, and the pull ring 151 at the bottom of amino acid bottle 15 must be checked for tightness. If the liquid transfer speed of the solvent bottle slows down, replace the sealing ring after ruling out gas source and filter issues. Solvent bottles and waste liquid bottles must not be operated under pressure. When removing the solvent bottle for addition, disconnect the connectors and organize the pipelines first. During installation, pay attention to distinguishing between gas and liquid pipelines to avoid incorrect connections. During equipment operation, do not open the transparent protective covers 112 on both sides of the front to prevent liquid splashing and malfunctions. Control the force when removing reactor 13 to avoid damage. The entire solvent management system 2 is rationally laid out and easy to operate, operating in conjunction with reaction system 1 and control system to provide a stable and precise solvent supply guarantee for peptide synthesis.

[0037] Referring to Figure 7, the control system, as the core command unit of the 96-channel peptide synthesizer, is installed entirely within the matching control cabinet. It is deeply integrated with the reaction system 1 and the solvent management system 2 to achieve automated operation and precise control of the entire process. The control cabinet is equipped with hardware such as a power switch, power inlet, and data cable interface. The control method is mainly fully automatic computer control, which establishes a signal connection with the entire system through data cables, and issues commands to control the orderly execution of each process such as liquid addition, stirring, liquid drainage, and purging.

[0038] The entire machine adopts a fully automated control mode, with all tasks executed by the software system. After the control cabinet is powered on, the user can log in to access the control system software interface. The interface is clearly divided into three main modules: a 96-channel operating status area, a solvent area, and an amino acid area. The 96-channel area displays the operating progress, task status, and solvent pressure of channels 1-96 in real time in a matrix format. The solvent area intuitively presents the liquid level and pressure parameters of various solvents such as DMF, DCM, and PIPE. The amino acid area displays the volume and allocation channel information of 24 amino acid bottles, allowing operators to monitor the equipment's operating status in real time. The control system supports multi-dimensional function operation, enabling automatic / manual mode switching, lighting control, synthesis method editing, and project management. Users can customize parameters such as liquid addition volume, reaction time, and stirring speed according to process requirements. It supports simultaneous synthesis of all 96 channels or independent operation of a single channel. It also features user access control and data recording backup functions to ensure traceability and compliance with specifications during the synthesis process. Manual mode requires password verification to prevent accidental operation.

[0039] The control system forms a closed-loop linkage with reaction system 1 and solvent management system 2. Through software commands, it precisely controls the rotation and stirring of reaction rack 12, the on / off state of solvent valve 21, the precise alignment of liquid addition tower 14, and the dynamic adjustment of gas source pressure, achieving unattended operation of the entire peptide synthesis process. Regarding operating procedures, after confirming that all switches are off, connect the communication cable to the control cabinet first, and then connect the power cord last. The computer power switch must be used correctly to avoid accidental activation. During equipment operation, it is strictly forbidden to open the front transparent protective cover 112 to prevent liquid splashing and malfunctions. The entire control system features a high degree of automation, a user-friendly interface, and stable linkage performance, providing precise and reliable control for high-throughput peptide synthesis. A 96-channel peptide synthesis method is described below: First, equipment preparation is completed by installing 96 7ml monolayer PP reactors 13 at their corresponding positions on the reaction rack 12. Corresponding Fmoc-protected amino acid solutions are added to 24 170ml amino acid bottles 15 and secured with pull rings 151. Solvent lines such as DMF, DCM, PIPE, and condensing agent are connected to the gas lines according to the synthesizer piping diagram. The nitrogen pressure is adjusted to 5psi and the compressed air pressure to 60psi. After confirming no leaks, the control cabinet power is turned on. Then, the amino acid sequence and resin parameters are entered into the control system software. The synthesis process, including liquid addition, 360° oscillation stirring, liquid drainage, and nitrogen purging, is edited. Parameters such as the liquid addition volume and reaction time for each step are set, and the corresponding synthesis project is bound. The process is then started. Following this, the control system links reaction system 1 and solvent management system 2, with 12 liquid addition towers 14 operating in a staggered manner. Through positive nitrogen pressure and the coordinated operation of the liquid addition pump and transfer bottle 22, the solvent and amino acid solution, metered by cylinder tapping, are precisely added to the corresponding reactor 13. Reactor 13, driven by reaction rack 12, oscillates and stirs 360° to complete the coupling reaction. After the reaction, the liquid is simultaneously drained and the filter plate 133 is purged through positive nitrogen pressure. The deprotection, washing, and coupling processes are repeated until the peptide chain synthesis is completed. After synthesis, reactor 13 is removed, inverted, and the peptide resin is collected. Reactor 13 and pipelines are cleaned sequentially with DMF and methanol to complete equipment maintenance. This method can achieve simultaneous high-throughput synthesis with 96 channels, good batch consistency, and a high degree of automation, and is suitable for peptide library construction and process optimization.

[0040] The operating principle of this invention is as follows: Using nitrogen positive pressure as the core power source, the control system coordinates and links the reaction system 1 and the solvent management system 2 to achieve high-throughput automated peptide synthesis. The liquid addition tower 14 is the core liquid supply unit. During liquid addition, the reactor 13 rotates to the position below the corresponding liquid addition tower 14. The liquid addition tower 14 descends and opens its valve, allowing precise delivery of solvent via the transfer bottle 22 and the liquid addition pump. Residual solvent is then purged into the reactor 13 with nitrogen. Multiple liquid addition towers 14 operate in a staggered manner, shortening the liquid addition waiting time. Amino acids are metered using a cylinder tapping method, ensuring precise addition as needed. The reactor 13 achieves thorough reaction through oscillation and stirring, while the lower cover filter plate 133 retains peptide resin. Nitrogen positive pressure drives simultaneous liquid drainage through multiple channels. The control system monitors the status of each channel in real time via software, allows for customized process parameters, and automates the entire process of liquid addition, stirring, drainage, and purging cycles to complete peptide chain synthesis, combining high throughput, high precision, and automation.

[0041] Obviously, the described embodiments are only some, not all, of the embodiments of the present invention. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without inventive effort are within the scope of protection of the present invention.

Claims

1. A 96-channel peptide synthesizer, characterized in that: The system includes a reaction system (1), a solvent management system (2), and a control system. The reaction system (1) includes a housing (11), a reaction rack (12), a reactor (13), a liquid addition tower (14), and an amino acid bottle (15). A partition (111) is fixed inside the housing (11). Above the partition (111) is a hollow space for installing the liquid addition tower (14) and the amino acid bottle (15). The reaction rack (12) is fixed on the partition (111). The reaction rack (12) uses an upper lid for liquid addition and a lower lid for liquid discharge. The reactor (13) can be oscillated and stirred 360°.

2. The 96-channel peptide synthesizer according to claim 1, characterized in that: The reactor (13) is a detachable structure. A handle (134) is integrally formed on the outside of the reactor (13). A filter plate (133) is embedded inside the reactor (13). A solvent inlet (131) is opened at the top of the reactor (13) and a drain outlet (132) is opened at the bottom.

3. The 96-channel peptide synthesizer according to claim 1, characterized in that: The amino acid bottle (15) is arranged above the reactor (13), and the bottom is provided with a pull ring (151) quick-release structure and a cylinder tapping metering mechanism.

4. The 96-channel peptide synthesizer according to claim 1, characterized in that: The liquid addition tower (14) is fixedly installed in the hollow area above the partition plate (111). There are multiple sets of the liquid addition tower (14). The multiple sets of liquid addition tower (14) are staggered. Each set of liquid addition tower (14) corresponds to multiple reactors (13). It can be connected to multiple solvent and nitrogen pipelines at the same time to realize multi-channel synchronous and precise liquid addition and pipeline residual solvent purging.

5. The 96-channel peptide synthesizer according to claim 1, characterized in that: The solvent management system (2) includes a liquid pump, a solvent valve (21), and a transfer bottle (22). The transfer bottle (22) is fixedly installed on the partition (111) of the box (11) for transferring and storing solvent and stabilizing the liquid supply pressure. The liquid pump connects the transfer bottle (22) and the liquid tower (14) for accurately delivering the solvent in the transfer bottle (22) to the reactor (13). The solvent valve (21) is centrally arranged below the partition (111) on the front side of the box (11) for controlling the on / off state and flow switching of each solvent.

6. The 96-channel peptide synthesizer according to claim 1, characterized in that: The addition and discharge of liquid in the synthesizer are both driven by positive pressure of nitrogen. The bottom of the box (11) is provided with a gas / liquid interface (114) and is connected to the reactor (13) for connecting nitrogen, compressed air and solvent pipelines.

7. The 96-channel peptide synthesizer according to claim 1, characterized in that: The control system adopts a fully automatic computer control mode, which can monitor the operating status of 96 channels in real time, edit peptide synthesis process parameters, manage user permissions and backup synthesis data, and supports switching between automatic operation mode and password-protected manual operation mode.

8. The 96-channel peptide synthesizer according to claim 1, characterized in that: The top of the box (11) is rotatably connected to a rotary button (113) for manually adjusting the alignment angle between the reaction rack (12) and the liquid addition tower (14). The front side of the box (11) is provided with a valve and a transparent protective cover (112).

9. The 96-channel peptide synthesizer according to claim 1, characterized in that: The transfer bottle (22) is equipped with a photoelectric sensor on the outside for real-time monitoring of solvent level and prevention of overflow. The solvent valve (21) is made of corrosion-resistant material and is adapted to control the on / off of various solvents required for peptide synthesis.

10. A method for synthesizing a 96-channel polypeptide as described in any one of claims 1-9, characterized in that: Includes the following steps: Install reactor (13), fix amino acid bottle (15), connect solvent and gas pipeline and adjust pressure; enter synthesis parameters and edit synthesis process in control system; control system link reaction system (1) and solvent management system (2) to automatically complete liquid addition, oscillation stirring, liquid discharge, nitrogen purging cycle until polypeptide chain synthesis is completed; disassemble reactor (13) to collect peptide resin, and clean reactor (13) and pipeline.

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