Pdrn-amino acid composite toothpaste and preparation method thereof
By compounding spirulina PDRN with glycine and arginine in toothpaste and using a specific process, the problems of PDRN permeability and amino acid dispersion were solved, resulting in a mild, non-irritating, fine-foaming, and highly stable multifunctional toothpaste suitable for diverse oral care needs.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- GUANGZHOU QIANCAI COSMETIC CO LTD
- Filing Date
- 2026-04-16
- Publication Date
- 2026-06-30
Smart Images
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Abstract
Description
Technical Field
[0001] This invention relates to the field of oral care technology, and in particular to a PDRN-amino acid complex toothpaste and its preparation method. Background Technology
[0002] Toothpaste is an essential product for daily oral hygiene. As consumers become more health-conscious, their demand for toothpaste has expanded from basic cleaning and cavity prevention to more diverse areas such as gum care, oral mucosal repair, anti-sensitivity, and anti-inflammation.
[0003] Polydeoxyribonucleotides (PDRNs) are a class of DNA-derived fragments with tissue repair and anti-inflammatory activities. Currently, commercially available PDRNs are mainly extracted from salmon germ cells, and their molecular weight is relatively large (usually greater than 100 kDa). In toothpaste, a system with a short residence time (only a few minutes) and high water content, it is difficult for them to penetrate and act on the oral mucosa in a short time, thus limiting their efficacy. In addition, sodium lauryl sulfate (SLS), a foaming agent widely used in toothpaste, although it has strong cleaning power and produces rich foam, can irritate the oral mucosa to varying degrees, and long-term use may aggravate oral sensitivity or ulcer problems.
[0004] In pursuit of gentler ingredients, amino acid surfactants such as lauroyl alanine have been introduced into toothpaste. However, lauroyl alanine is easily affected by temperature, pH, and salt ion concentration in aqueous solutions, forming micelles. This results in difficulty in dispersion and poor solubility during toothpaste preparation, severely impacting paste uniformity and production feasibility. Current technologies often react it with sodium hydroxide to produce a sodium salt, but this approach does not fundamentally solve the micelle problem and fails to fully utilize the potential oral care benefits of amino acids.
[0005] Therefore, developing a new type of toothpaste that is highly effective in repairing, gentle and non-irritating, and has a stable manufacturing process has significant market value and technological challenges. Summary of the Invention
[0006] The present invention aims to address the shortcomings of the prior art by providing a PDRN-amino acid composite toothpaste and its preparation method. This toothpaste achieves excellent mucosal repair, anti-inflammatory, and antibacterial effects by combining PDRN from specific spirulina with an innovatively designed surfactant system, while also possessing excellent mildness, foaming properties, and paste stability.
[0007] To achieve the above objectives, the present invention adopts the following technical solution:
[0008] In a first aspect, the present invention provides a PDRN-amino acid complex toothpaste, comprising, by weight percentage: 20%~60% humectant, 15%~25% abrasive, 1%~4% foaming agent, 0.1%~2% sweetener, 0.5%~1.5% thickener, 0.8%~1.8% fragrance, 0.1%~0.5% preservative, 0.1%~1.5% main active ingredient, 0.1%~3% auxiliary active ingredient, and the balance being water. The main active ingredient comprises glycine and polydeoxyribonucleotide derived from spirulina (abbreviated as spirulina PDRN). The foaming agent comprises lauroyl alanine arginine salt and alkyl glycoside, wherein the weight ratio of lauroyl alanine arginine salt to the alkyl glycoside is 1:3 to 3:1.
[0009] As a preferred technical solution, the polydeoxyribonucleotide derived from spirulina (abbreviated as spirulina PDRN) has a small molecular weight, is a short chain, and has a fragment length of 50~100bp.
[0010] As a preferred technical solution, in the foaming agent combination, the weight ratio of lauroyl alanine arginine salt to alkyl glycoside is 1:1.
[0011] In some specific technical solutions, the lauroyl alanine arginine salt is prepared by neutralizing lauroyl alanine and L-arginine in water, and the resulting aqueous solution of lauroyl alanine arginine salt has a mass fraction of 20 wt% and a pH of 6.30~7.30.
[0012] As a preferred technical solution, the auxiliary functional ingredient is selected from one or more of zinc citrate, sodium phytate, tetrasodium pyrophosphate, sodium monofluorophosphate, and sodium fluoride.
[0013] As a preferred technical solution, the moisturizer is selected from one or more of sorbitol, glycerin, propylene glycol, and polyethylene glycol.
[0014] As a preferred technical solution, the friction agent is selected from one or more of hydrated silica, calcium hydrogen phosphate dihydrate, and anhydrous calcium hydrogen phosphate.
[0015] As a preferred technical solution, the sweetener is selected from one or more of sucralose, trehalose, sodium saccharin, and dipotassium glycyrrhizate.
[0016] As a preferred technical solution, the thickener is selected from one or more of xanthan gum, cellulose gum, and hydroxypropyl guar gum.
[0017] As a preferred technical solution, the preservative is selected from one or more of methylparaben, sodium benzoate, and potassium sorbate.
[0018] Secondly, the present invention also provides a method for preparing any of the above-mentioned PDRN-amino acid complex toothpastes, comprising the following steps:
[0019] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Lauroyl alanine and L-arginine were neutralized in water to prepare lauroyl alanine arginine salt aqueous solution;
[0020] (2) Preparation of pre-dissolved solution 1: Dissolve the sweetener, main active ingredient, preservative and water-soluble auxiliary active ingredient in a portion of water, heat and stir until completely dissolved to obtain pre-dissolved solution 1;
[0021] (3) Preparation of pre-dissolving solution 2: Mix and dissolve the first part of the moisturizer, the lauroyl alanine arginine salt aqueous solution obtained in step (1), the alkyl glycoside and the remaining water to obtain pre-dissolving solution 2;
[0022] (4) Preparation of pre-dispersion: Add the thickener to the second part of the humectant, stir and disperse to obtain the pre-dispersion;
[0023] (5) Preparation of toothpaste base: Mix pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution, let stand to swell, and obtain toothpaste base;
[0024] (6) Mix the toothpaste gel base with the abrasive and homogenize;
[0025] (7) Add fragrance and continue homogenization. After defoaming, the finished paste is obtained.
[0026] As a preferred technical solution, in step (1), the temperature of the neutralization reaction is 60℃~70℃, and it is supplemented with ultrasonic treatment for 10min~20min, with ultrasonic power of 150W~250W.
[0027] As a preferred technical solution, in step (2), the heating and stirring is carried out at 40℃~65℃ and 800rpm~1000rpm for 10min~25min.
[0028] As a preferred technical solution, in step (3), the mixing and dissolving is carried out by stirring at 800 rpm to 1000 rpm for 8 min to 15 min at room temperature.
[0029] As a preferred technical solution, in step (4), the stirring and dispersing is carried out at 800 rpm to 1000 rpm for 2 min to 8 min at room temperature.
[0030] As a preferred technical solution, in step (3), the first part of the moisturizer accounts for 60% to 80% of the total moisturizer mass; in step (4), the second part of the moisturizer accounts for 20% to 40% of the total moisturizer mass.
[0031] As a preferred technical solution, in step (5), the mixing is carried out by stirring at 800 rpm to 1000 rpm for 20 min to 40 min, and the standing swelling time is more than 8 hours.
[0032] As a preferred technical solution, in steps (6) and (7), the stirring speed for homogenization is 800 rpm to 1200 rpm and the homogenization time is 4 min to 18 min.
[0033] Compared with the prior art, the present invention provides a PDRN-amino acid complex toothpaste and its preparation method, which has the following beneficial effects:
[0034] (1) Innovative combination of core efficacy and synergistic effect: This invention is the first to combine small molecule PDRN from spirulina with glycine and arginine in toothpaste. Spirulina PDRN has a small molecular weight (fragment length of 50~100bp), which, compared with traditional fish-derived PDRN (usually >100kDa), has better transdermal absorption and water activity retention rate, and can quickly act on damaged mucosa during the short brushing time to inhibit inflammatory aging of gingival fibroblasts. Glycine, as a key amino acid for collagen synthesis, synergistically promotes tissue repair with spirulina PDRN. Arginine is not only key to solving the surfactant problem, but it also has the effect of promoting hydroxyapatite deposition and sealing dentinal tubules. The three together construct a three-dimensional oral care system of "rapid repair - nutritional support - remineralization protection".
[0035] (2) Breakthrough Mild Surfactant System: This invention creatively uses the reaction of L-arginine and lauroyl alanine to generate lauroyl alanine arginine salt, fundamentally solving the technical problem that lauroyl alanine easily forms micelles and is difficult to disperse during toothpaste preparation. Compared with the sodium lauroyl alanine salt commonly used in the prior art, the aqueous solution of the lauroyl alanine arginine salt of this invention has higher clarity and better low-temperature stability. Furthermore, when compounded with alkyl glycosides (APG) in a specific ratio, a significant foam synergistic effect is produced, resulting in dense foam with a foam volume (>160mm) comparable to the traditional foaming agent sodium lauryl sulfate (SLS), and extremely low irritation. Oral mucosal irritation tests have confirmed that the toothpaste of this invention has no irritating reaction to the mucosa, is safe and non-toxic, and is suitable for long-term use by people with sensitive mouths.
[0036] (3) Excellent paste stability: Through a carefully designed "stepwise dissolution-pre-dispersion-vacuum paste making" process, especially the introduction of lauroyl alanine arginine salt, this invention completely avoids the phenomena of paste thinning, water separation, and separation that occur in traditional amino acid surfactant pastes during heat resistance (45℃) and cold resistance (-18℃) tests. After 3 months of accelerated aging test, the toothpaste of this invention still remains uniform, odorless, and without separation, demonstrating excellent shelf stability.
[0037] (4) Multiple oral health benefits: Experiments have shown that the toothpaste of this invention can not only effectively inhibit major oral pathogens (such as Porphyromonas gingivalis and Streptococcus mutans), but also significantly reduce the expression of inflammatory cytokines (such as IL-6 and TNF-α), promote the migration of gingival fibroblasts, and achieve rapid repair of the oral mucosa. At the same time, the presence of arginine gives it excellent dentinal tubule sealing ability, which can effectively relieve tooth sensitivity. Detailed Implementation
[0038] The technical solution of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.
[0039] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this invention, "a plurality of" and "multiple" mean two or more, unless otherwise explicitly specified.
[0040] This invention provides a PDRN-amino acid complex toothpaste, comprising the following components by weight percentage: 20%~60% humectant, 15%~25% abrasive, 1%~4% foaming agent combination, 0.1%~2% sweetener, 0.5%~1.5% thickener, 0.8%~1.8% fragrance, 0.1%~0.5% preservative, 0.1%~1.5% main active ingredient, 0.1%~3% auxiliary active ingredient, and water as the balance.
[0041] The main active ingredients include glycine and polydeoxyribonucleotides derived from spirulina (abbreviated as spirulina PDRN). The fragment length of spirulina PDRN is 50-100 bp. This invention uses spirulina PDRN to replace fish-derived PDRN. Spirulina is a cyanobacterium, a multicellular, filamentous, prokaryotic organism, also known as a cyanobacterium, and possesses antioxidant and anti-inflammatory properties. Because spirulina PDRN is a small, short-chain molecule, it is more easily absorbed and retained, and it remains active in water. Therefore, even at low concentrations, it can significantly repair the oral barrier and repair inflammatory aging of gingival fibroblasts.
[0042] The foaming agent composition comprises lauroyl alanine arginine salt and alkyl glycoside, with a weight ratio of lauroyl alanine arginine salt to alkyl glycoside of 1:3 to 3:1. Lauroyl alanine arginine salt is prepared by neutralizing lauroyl alanine with L-arginine in water, resulting in an aqueous solution of lauroyl alanine arginine salt with a mass fraction of 20 wt% and a pH of 6.30–7.30. This invention selects to react lauroyl alanine with L-arginine, one of the 20 basic amino acids. L-arginine contains an amino group in its molecular structure, is basic, and can react with acids to form salts. The carboxyl group in lauroyl alanine and the guanidinyl group in L-arginine undergo an acid-base neutralization reaction to obtain an aqueous solution of lauroyl alanine arginine salt, solving the micelle problem. Furthermore, arginine in the oral cavity also promotes hydroxyapatite deposition, seals dentinal tubules, and prevents tooth decay. This results in a surfactant with fine, mild, and non-irritating foam, while also enhancing the efficacy of toothpaste.
[0043] The auxiliary active ingredient is selected from one or more of zinc citrate, sodium phytate, tetrasodium pyrophosphate, sodium monofluorophosphate, and sodium fluoride. When zinc citrate is selected as the auxiliary active ingredient, it must be added together with the abrasive.
[0044] The moisturizer is selected from one or more of sorbitol, glycerin, propylene glycol, and polyethylene glycol.
[0045] The abrasive is selected from one or more of hydrated silica, calcium hydrogen phosphate dihydrate, and anhydrous calcium hydrogen phosphate.
[0046] The sweetener is selected from one or more of sucralose, trehalose, sodium saccharin, and dipotassium glycyrrhizate.
[0047] The thickener is selected from one or more of xanthan gum, cellulose gum, and hydroxypropyl guar gum.
[0048] The preservative is selected from one or more of methylparaben, sodium benzoate, and potassium sorbate.
[0049] Furthermore, the preparation method of PDRN-amino acid complex toothpaste includes the following steps:
[0050] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Add lauroyl alanine and some water to a reaction vessel, heat to 60℃~70℃ and sonicate (ultrasonic power 150W~250W, 10min~20min) until lauroyl alanine is completely dispersed (no particles or agglomerates); while hot, quickly add L-arginine, stir to carry out neutralization reaction, adjust pH to 6.30~7.30, cool and add water to the target concentration to obtain a 20wt% lauroyl alanine arginine salt aqueous solution. The purpose of this step is to utilize the basic guanidinium group of arginine to react with the carboxyl group of lauroyl alanine to form an ionic salt, thereby breaking its micellar aggregation state in aqueous solution and obtaining a clear, easily dispersible surfactant solution.
[0051] (2) Preparation of pre-dissolved solution 1: Dissolve the sweetener, main active ingredient (spirulina PDRN, glycine), preservative, and water-soluble auxiliary active ingredient in a portion of water, and heat and stir at 800 rpm to 1000 rpm at 40℃ to 65℃ until completely dissolved (approximately 10 min to 25 min) to obtain pre-dissolved solution 1. The purpose of this step is to ensure that trace amounts of key water-soluble active ingredients (especially bioactive molecules such as PDRN) are fully and uniformly dissolved under mild conditions, avoiding the destruction of their activity by high temperature or strong shear force.
[0052] (3) Preparation of pre-dissolved solution 2: The first part of the moisturizer (accounting for 60%~80% of the total moisturizer mass), the lauroyl alanine arginine salt aqueous solution obtained in step (1), the alkyl glycoside and the remaining water are mixed and dissolved at room temperature at 800rpm~1000rpm for 8min~15min to obtain pre-dissolved solution 2. The purpose of this step is to achieve pre-hydration and uniform dispersion of the foaming agent combination, so as to provide a good initial distribution for its subsequent fusion with other components of the paste.
[0053] (4) Preparation of pre-dispersion: Add the thickener to the second part of the humectant (accounting for 20%~40% of the total humectant mass), and stir and disperse at 800rpm~1000rpm for 2min~8min at room temperature to obtain the pre-dispersion. The purpose of this step is to use the high viscosity of the humectant to fully wet and disperse the thickener powder, preventing it from forming insoluble "fish-eye" agglomerates when directly added to water.
[0054] (5) Preparation of toothpaste base: Mix pre-dissolved liquid 1, pre-dissolved liquid 2 and pre-dispersed liquid, stir at 800 rpm to 1000 rpm for 20 min to 40 min, and then let stand for more than 8 hours to allow the thickener to fully swell in the toothpaste base, resulting in a uniform toothpaste base. The purpose of this step is to allow all liquid phase components to fully integrate and to give the thickener sufficient time to build a stable three-dimensional network structure, providing an ideal rheological basis for the subsequent addition of abrasives.
[0055] (6) Paste preparation: Turn on the vacuum pump of the paste preparation machine, and suck the toothpaste base and abrasive obtained in step (5) into the paste preparation pot. Under vacuum conditions (vacuum degree above -0.091MPa), turn on the stirring and scraper, and homogenize at 800rpm~1200rpm for 4min~12min. The purpose of this step is to evenly disperse the powder such as abrasive into the base under vacuum, and at the same time remove the air bubbles in the paste by negative pressure to ensure that the paste is dense and smooth.
[0056] (7) Fragrance addition and refining: The fragrance is drawn into the paste-making pot and homogenized at 800 rpm to 1200 rpm for 4 to 6 minutes under vacuum conditions (vacuum degree above -0.091 MPa); then the vacuum degree is increased to above -0.095 MPa, and homogenization is continued for 8 to 12 minutes to obtain the finished paste. The purpose of this step is to ensure that the fragrance is evenly dispersed and to further defoam, so as to obtain a final product with a delicate appearance and no bubbles.
[0057] (8) Filling: Fill the toothpaste tube with the finished paste.
[0058] The present invention will be further described in detail below through detailed embodiments.
[0059] Unless otherwise specified, the experimental methods used in the examples and comparative examples are conventional methods, and the raw materials, reagents, biological materials, etc. used are commercially available products that can be obtained through commercial channels.
[0060] The raw material sources used in the embodiments and comparative examples of this invention are as follows: Spirulina PDRN was purchased from Hemei Biotechnology (Zhejiang) Co., Ltd. (model: Yuesource PDRN, fragment length 50~100bp). Fish-derived PDRN was purchased from Nanjing Shihe Gene Biotechnology Co., Ltd. (extracted from salmon germ cells, molecular weight >100kDa). Glycine was purchased from Anhui Huaheng Biotechnology Co., Ltd. Lauroyl alanine (purity >98%) was purchased from Suzhou Weimei Biotechnology Co., Ltd. Alkyl glycoside aqueous solution (APG, 50wt%, model APG 0814) was purchased from BASF (China) Co., Ltd. L-arginine (food grade, purity >99%) was purchased from Suzhou Weimei Biotechnology Co., Ltd.
[0061] Example 1
[0062] This embodiment provides a PDRN-amino acid complex toothpaste, whose formula composition by weight percentage includes: 30.8% sorbitol, 9% glycerin, 18% hydrated silica, 1.2% lauroyl alanine arginine salt, 1.2% alkyl glycoside, 0.3% sucralose, 1% trehalose, 0.7% xanthan gum, 0.3% cellulose gum, 1.1% fragrance, 0.15% methylparaben, 0.13% spirulina PDRN, 0.2% glycine, 0.4% sodium phytate, 0.5% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0063] The preparation method of the PDRN-amino acid complex toothpaste in this embodiment includes the following steps:
[0064] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Add 20g of lauroyl alanine and 50g of deionized water to a 150mL beaker, heat to 65℃ and ultrasonically disperse for 15min (ultrasonic power of 200W) until lauroyl alanine is completely dispersed (without particles or agglomerates) and forms a milky white uniform suspension; while hot, quickly add 5.2g of L-arginine, stir to carry out neutralization reaction, and monitor pH until pH stabilizes at 6.8. After cooling to room temperature, add deionized water to 100g to obtain 100g of clear lauroyl alanine arginine salt aqueous solution with a 20wt% concentration.
[0065] (2) Preparation of pre-dissolving solution 1: Dissolve 0.3g of sucralose, 1g of trehalose, 0.13g of spirulina PDRN, 0.2g of glycine, 0.15g of methylparaben, 0.4g of sodium phytate and 0.5g of tetrasodium pyrophosphate in 15g of deionized water, heat to 60℃, and stir at 900rpm for 15min until completely dissolved to obtain pre-dissolving solution 1.
[0066] (3) Preparation of pre-dissolving solution 2: 44g of sorbitol aqueous solution (70wt%), 6g of lauroyl alanine arginine salt aqueous solution (20wt%) obtained in step (1), 2.4g of alkyl glycoside aqueous solution (50wt%) and the remaining 20.02g of deionized water are mixed and dissolved at 900rpm for 10min at room temperature to make them evenly mixed and obtain pre-dissolving solution 2.
[0067] (4) Preparation of pre-dispersion: Add 0.7g xanthan gum and 0.3g cellulose gum to 9g glycerol, and stir at 1000rpm for 5min at room temperature to disperse evenly and obtain pre-dispersion.
[0068] (5) Preparation of toothpaste base: Pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution are mixed in a reactor and stirred at 900 rpm for 30 min. Then, stirring is stopped and the mixture is allowed to stand and swell for 10 hours to obtain a uniform toothpaste base.
[0069] (6) Paste making: Turn on the vacuum pump of the paste making machine, suck the toothpaste base obtained in step (5) and 18g of hydrated silica into the paste making pot, turn on the stirring and scraper, and homogenize at 1000rpm for 10min under vacuum conditions (vacuum degree is -0.091MPa).
[0070] (7) Fragrance addition and refining: 1.1g of fragrance is drawn into the ointment pot and homogenized at 1000rpm for 5min under vacuum conditions (vacuum degree is -0.091MPa); then the vacuum degree is increased to -0.095MPa and homogenized for another 10min to obtain the finished ointment.
[0071] (8) Filling: Fill the finished paste into the aluminum-plastic composite tube.
[0072] Example 2
[0073] This embodiment provides a toothpaste containing PDRN-amino acid complex. The difference between this embodiment and Embodiment 1 is that the amount of spirulina PDRN and glycine is reduced, and the ratio of the foaming agent combination is adjusted (the ratio of lauroyl alanine arginine salt to alkyl glycoside is 1:2.5).
[0074] Specifically, by weight percentage, the toothpaste formulation of this embodiment containing PDRN-amino acid complex comprises: 28% sorbitol, 8% glycerin, 20% hydrated silica, 0.6% lauroyl alanine arginine salt, 1.5% alkyl glycoside, 0.2% sucralose, 0.5% trehalose, 0.6% xanthan gum, 0.2% cellulose gum, 1% fragrance, 0.15% methylparaben, 0.05% spirulina PDRN, 0.1% glycine, 0.3% sodium phytate, 0.4% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0075] The preparation method of the PDRN-amino acid complex toothpaste in this embodiment includes the following steps:
[0076] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Same as step (1) in Example 1, to obtain 20wt% clear lauroyl alanine arginine salt aqueous solution.
[0077] (2) Preparation of pre-dissolving solution 1: Dissolve 0.2g of sucralose, 0.5g of trehalose, 0.05g of spirulina PDRN, 0.1g of glycine, 0.15g of methylparaben, 0.3g of sodium phytate and 0.4g of tetrasodium pyrophosphate in 12g of deionized water, heat to 55℃, and stir at 850rpm for 20min until completely dissolved to obtain pre-dissolving solution 1.
[0078] (3) Preparation of pre-dissolving solution 2: 40g of sorbitol aqueous solution (70wt%), 3g of lauroyl alanine arginine salt aqueous solution (20wt%) obtained in step (1), 3g of alkyl glycoside aqueous solution (50wt%) and the remaining 26.4g of deionized water are mixed and dissolved at 900rpm for 12min at room temperature to make them evenly mixed and obtain pre-dissolving solution 2.
[0079] (4) Preparation of pre-dispersion: Add 0.6g xanthan gum and 0.2g cellulose gum to 8g glycerol, and stir and disperse at 1000rpm for 5min at room temperature to make it evenly dispersed to obtain pre-dispersion.
[0080] (5) Preparation of toothpaste base: Pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution are mixed in a reactor and stirred at 900 rpm for 30 min. Then, stirring is stopped and the mixture is allowed to stand and swell for 12 hours to obtain a uniform toothpaste base.
[0081] (6) Paste making: Turn on the vacuum pump of the paste making machine, suck the toothpaste base obtained in step (5) and 20g of hydrated silica into the paste making pot, turn on the stirring and scraper, and homogenize at 1000rpm for 10min under vacuum conditions (vacuum degree is -0.091MPa).
[0082] (7) Fragrance addition and refining: 1g of fragrance is drawn into the ointment pot and homogenized at 1000rpm for 5min under vacuum conditions (vacuum degree is -0.091MPa); then the vacuum degree is increased to -0.095MPa and homogenized for another 10min to obtain the finished ointment.
[0083] (8) Filling: Fill the finished paste into the aluminum-plastic composite tube.
[0084] Example 3
[0085] This embodiment provides a toothpaste containing PDRN-amino acid complex. The difference between this embodiment and Embodiment 1 is that the amount of spirulina PDRN and glycine is increased, and the ratio of the foaming agent combination is adjusted (the ratio of lauroyl alanine arginine salt to alkyl glycoside is 3:2.5).
[0086] Specifically, by weight percentage, the toothpaste formulation of this embodiment containing PDRN-amino acid complex includes: 24.5% sorbitol, 10% glycerin, 22% hydrated silica, 1.8% lauroyl alanine arginine salt, 1.5% alkyl glycoside, 0.4% sucralose, 1.5% trehalose, 0.8% xanthan gum, 0.4% cellulose gum, 1.5% fragrance, 0.2% methylparaben, 0.5% spirulina PDRN, 0.8% glycine, 0.5% sodium phytate, 0.6% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0087] The preparation method of the PDRN-amino acid complex toothpaste in this embodiment includes the following steps:
[0088] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Same as step (1) in Example 1, to obtain 20wt% clear lauroyl alanine arginine salt aqueous solution.
[0089] (2) Preparation of pre-dissolving solution 1: Dissolve 0.4g of sucralose, 1.5g of trehalose, 0.5g of spirulina PDRN, 0.8g of glycine, 0.2g of methylparaben, 0.5g of sodium phytate and 0.6g of tetrasodium pyrophosphate in 20g of deionized water, heat to 65℃, and stir at 950rpm for 20min until completely dissolved to obtain pre-dissolving solution 1.
[0090] (3) Preparation of pre-dissolving solution 2: 35g of sorbitol aqueous solution (70wt%), 9g of lauroyl alanine arginine salt aqueous solution (20wt%) obtained in step (1), 3g of alkyl glycoside aqueous solution (50wt%) and the remaining 13g of deionized water are mixed and dissolved at 900rpm for 10min at room temperature to make them evenly mixed and obtain pre-dissolving solution 2.
[0091] (4) Preparation of pre-dispersion: Add 0.8g xanthan gum and 0.4g cellulose gum to 10g glycerol, and stir at 1000rpm for 5min at room temperature to disperse evenly and obtain pre-dispersion.
[0092] (5) Preparation of toothpaste base: Pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution are mixed in a reactor and stirred at 950 rpm for 35 min. Then, stirring is stopped and the mixture is allowed to stand and swell for 10 hours to obtain a uniform toothpaste base.
[0093] (6) Paste making: Turn on the vacuum pump of the paste making machine, suck the toothpaste base obtained in step (5) and 22g of hydrated silica into the paste making pot, turn on the stirring and scraper, and homogenize at 1100rpm for 5min under vacuum conditions (vacuum degree is -0.091MPa).
[0094] (7) Fragrance addition and refining: 1.5g of fragrance is drawn into the ointment pot and homogenized at 1100rpm for 5min under vacuum conditions (vacuum degree is -0.091MPa); then the vacuum degree is increased to -0.095MPa and homogenized for another 10min to obtain the finished ointment.
[0095] (8) Filling: Fill the finished paste into the aluminum-plastic composite tube.
[0096] Example 4
[0097] This embodiment provides a PDRN-amino acid complex toothpaste. The difference between this embodiment and Embodiment 1 is that sodium monofluorophosphate is added as an auxiliary active ingredient, and the ratio of the foaming agent combination is adjusted (the ratio of lauroyl alanine arginine salt to alkyl glycoside is 1:2.5).
[0098] Specifically, by weight percentage, the toothpaste formulation of this embodiment containing PDRN-amino acid complex includes: 29.4% sorbitol, 9% glycerin, 19% hydrated silica, 0.8% lauroyl alanine arginine salt, 2% alkyl glycoside, 0.3% sucralose, 1% trehalose, 0.7% xanthan gum, 0.3% cellulose gum, 1.2% fragrance, 0.15% methylparaben, 0.13% spirulina PDRN, 0.2% glycine, 0.3% sodium phytate, 0.4% tetrasodium pyrophosphate, 0.8% sodium monofluorophosphate, and the balance being deionized water, totaling 100%.
[0099] The preparation method of the PDRN-amino acid complex toothpaste in this embodiment includes the following steps:
[0100] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Same as step (1) in Example 1, to obtain 20wt% clear lauroyl alanine arginine salt aqueous solution.
[0101] (2) Preparation of pre-dissolving solution 1: Dissolve 0.3g of sucralose, 1g of trehalose, 0.13g of spirulina PDRN, 0.2g of glycine, 0.15g of methylparaben, 0.3g of sodium phytate, 0.4g of tetrasodium pyrophosphate and 0.8g of sodium monofluorophosphate in 18g of deionized water, heat to 60℃, and stir at 900rpm for 20min until completely dissolved to obtain pre-dissolving solution 1.
[0102] (3) Preparation of pre-dissolving solution 2: 42g of sorbitol aqueous solution (70wt%), 4g of lauroyl alanine arginine salt aqueous solution (20wt%) obtained in step (1), 4g of alkyl glycoside aqueous solution (50wt%) and the remaining 16.32g of deionized water are mixed and dissolved at 900rpm for 10min at room temperature to make them evenly mixed and obtain pre-dissolving solution 2.
[0103] (4) Preparation of pre-dispersion: Add 0.7g xanthan gum and 0.3g cellulose gum to 9g glycerol, and stir at 1000rpm for 5min at room temperature to disperse evenly and obtain pre-dispersion.
[0104] (5) Preparation of toothpaste base: Pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution are mixed in a reactor and stirred at 900 rpm for 30 min. Then, stirring is stopped and the mixture is allowed to stand and swell for 10 hours to obtain a uniform toothpaste base.
[0105] (6) Paste making: Turn on the vacuum pump of the paste making machine, suck the toothpaste base obtained in step (5) and 19g of hydrated silica into the paste making pot, turn on the stirring and scraper, and homogenize at 1000rpm for 10min under vacuum conditions (vacuum degree is -0.091MPa).
[0106] (7) Fragrance addition and refining: 1.2g of fragrance is drawn into the ointment pot and homogenized at 1000rpm for 5min under vacuum conditions (vacuum degree is -0.091MPa); then the vacuum degree is increased to -0.095MPa and homogenized for another 10min to obtain the finished ointment.
[0107] (8) Filling: Fill the finished paste into the aluminum-plastic composite tube.
[0108] Example 5
[0109] This embodiment provides a PDRN-amino acid complex toothpaste. The difference between this embodiment and Embodiment 1 is that zinc citrate is added as an auxiliary functional ingredient (added together with the abrasive), while the proportion of the foaming agent combination remains unchanged (the ratio of lauroyl alanine arginine salt to alkyl glycoside is 1:1).
[0110] Specifically, by weight percentage, the toothpaste formulation of this embodiment with PDRN-amino acid complex includes: 30.8% sorbitol, 9% glycerin, 18% hydrated silica, 1.2% lauroyl alanine arginine salt, 1.2% alkyl glycoside, 0.3% sucralose, 1% trehalose, 0.7% xanthan gum, 0.3% cellulose gum, 1.1% fragrance, 0.15% methylparaben, 0.13% spirulina PDRN, 0.2% glycine, 0.5% zinc citrate, 0.4% sodium phytate, 0.5% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0111] The preparation method of the PDRN-amino acid complex toothpaste in this embodiment includes the following steps:
[0112] (1) Preparation of lauroyl alanine arginine salt aqueous solution: Same as step (1) in Example 1, to obtain 20wt% clear lauroyl alanine arginine salt aqueous solution.
[0113] (2) Preparation of pre-dissolving solution 1: Dissolve 0.3g of sucralose, 1g of trehalose, 0.13g of spirulina PDRN, 0.2g of glycine, 0.15g of methylparaben, 0.4g of sodium phytate and 0.5g of tetrasodium pyrophosphate in 15g of deionized water, heat to 60℃, and stir at 900rpm for 15min until completely dissolved to obtain pre-dissolving solution 1.
[0114] (3) Preparation of pre-dissolving solution 2: 44g of sorbitol aqueous solution (70wt%), 6g of lauroyl alanine arginine salt aqueous solution (20wt%) obtained in step (1), 2.4g of alkyl glycoside aqueous solution (50wt%) and the remaining 19.52g of deionized water are mixed and dissolved at 900rpm for 10min at room temperature to make them evenly mixed and obtain pre-dissolving solution 2.
[0115] (4) Preparation of pre-dispersion: Add 0.7g xanthan gum and 0.3g cellulose gum to 9g glycerol, and stir at 1000rpm for 5min at room temperature to disperse evenly and obtain pre-dispersion.
[0116] (5) Preparation of toothpaste base: Pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution are mixed in a reactor and stirred at 900 rpm for 30 min. Then, stirring is stopped and the mixture is allowed to stand and swell for 10 hours to obtain a uniform toothpaste base.
[0117] (6) Paste making: Turn on the vacuum pump of the paste making machine, and suck the toothpaste base obtained in step (5), 18g of hydrated silica, and 0.5g of zinc citrate into the paste making pot. Turn on the stirring and scraper, and homogenize at 1000rpm for 10min under vacuum conditions (vacuum degree is -0.091MPa).
[0118] (7) Fragrance addition and refining: 1.1g of fragrance is drawn into the ointment pot and homogenized at 1000rpm for 5min under vacuum conditions (vacuum degree is -0.091MPa); then the vacuum degree is increased to -0.095MPa and homogenized for another 10min to obtain the finished ointment.
[0119] (8) Filling: Fill the finished paste into the aluminum-plastic composite tube.
[0120] Comparative Example 1
[0121] This comparative example provides a toothpaste. The difference between this comparative example and Example 1 is that the foaming agent combination is replaced with the conventional foaming agent sodium lauryl sulfate (SLS), at an amount of 2.4% (the active ingredient content is equivalent to the total active ingredient content of the foaming agent combination in Example 1). The remaining components are the same as in Example 1.
[0122] Specifically, by weight percentage, the toothpaste formula in this comparative example comprises: 30.8% sorbitol, 9% glycerin, 18% hydrated silica, 2.4% sodium lauryl sulfate (SLS), 0.3% sucralose, 1% trehalose, 0.7% xanthan gum, 0.3% cellulose gum, 1.1% fragrance, 0.15% methylparaben, 0.13% spirulina PDRN, 0.2% glycine, 0.4% sodium phytate, 0.5% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0123] The toothpaste of this comparative example is prepared by the following steps:
[0124] (1) Preparation of pre-dissolving solution 1: Dissolve 0.3g of sucralose, 1g of trehalose, 0.13g of spirulina PDRN, 0.2g of glycine, 0.15g of methylparaben, 0.4g of sodium phytate and 0.5g of tetrasodium pyrophosphate in 15g of deionized water, heat to 60℃, and stir at 900rpm for 15min until completely dissolved to obtain pre-dissolving solution 1.
[0125] (2) Preparation of pre-dissolving solution 2: 44g of sorbitol aqueous solution (70wt%), 2.4g of sodium lauryl sulfate (SLS) and the remaining 20.02g of deionized water are mixed and dissolved at 900rpm for 10min at room temperature to make them evenly mixed, thus obtaining pre-dissolving solution 2.
[0126] The subsequent steps, such as “preparation of pre-dispersion liquid”, “preparation of toothpaste base”, “paste making”, “fragrance addition and refining”, and “filling”, are the same as in Example 1, so they will not be described again.
[0127] Comparative Example 2
[0128] This comparative example provides a toothpaste. The difference between this comparative example and Example 1 is that the spirulina PDRN is replaced with an equal amount of fish-derived PDRN (extracted from salmon reproductive cells, molecular weight >100 kDa). The remaining components are the same as in Example 1.
[0129] Specifically, by weight percentage, the toothpaste formulation of this comparative example comprises: 30.8% sorbitol, 9% glycerin, 18% hydrated silica, 1.2% lauroyl alanine arginine salt, 1.2% alkyl glycoside, 0.3% sucralose, 1% trehalose, 0.7% xanthan gum, 0.3% cellulose gum, 1.1% fragrance, 0.15% methylparaben, 0.13% fish-derived PDRN (molecular weight > 100 kDa), 0.2% glycine, 0.4% sodium phytate, 0.5% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0130] The preparation method of the toothpaste in this comparative example is the same as that in Example 1, so it will not be described again.
[0131] Comparative Example 3
[0132] This comparative example provides a toothpaste. The difference between this comparative example and Example 1 is that the aqueous solution of lauroyl alanine arginine salt is replaced with an aqueous solution of sodium lauroyl alanine salt (prepared by neutralization with sodium hydroxide). The remaining components are the same as in Example 1.
[0133] Specifically, by weight percentage, the toothpaste formulation of this comparative example comprises: 30.8% sorbitol, 9% glycerin, 18% hydrated silica, 1.2% sodium lauroyl alanine, 1.2% alkyl glycoside, 0.3% sucralose, 1% trehalose, 0.7% xanthan gum, 0.3% cellulose gum, 1.1% fragrance, 0.15% methylparaben, 0.13% spirulina PDRN, 0.2% glycine, 0.4% sodium phytate, 0.5% tetrasodium pyrophosphate, and the balance being deionized water, totaling 100%.
[0134] The toothpaste of this comparative example is prepared by the following steps:
[0135] (1) Preparation of sodium lauroyl alanine aqueous solution: Add 20g of lauroyl alanine and 50g of deionized water to a 150mL beaker, heat to 65℃ and ultrasonically disperse for 15min (ultrasonic power of 200W) until lauroyl alanine is completely dispersed (without particles or agglomerates) and forms a milky white uniform suspension; while hot, quickly add 2g of sodium hydroxide (prepared as a 30wt% solution), stir the reaction, and monitor the pH until the pH stabilizes at 7.0. After cooling to room temperature, add deionized water to 100g to obtain 100g of 20wt% sodium lauroyl alanine aqueous solution (the solution is slightly opalescent or slightly turbid).
[0136] (2) Preparation of pre-dissolving solution 1: Same as step (2) in Example 1, to obtain pre-dissolving solution 1.
[0137] (3) Preparation of pre-dissolving solution 2: 44g of sorbitol aqueous solution (70wt%), 6g of sodium lauroyl alanine salt aqueous solution (20wt%) obtained in step (1), 2.4g of alkyl glycoside aqueous solution (50wt%) and the remaining 20.02g of deionized water are mixed and dissolved at 900rpm for 10min at room temperature to make them evenly mixed and obtain pre-dissolving solution 2.
[0138] The subsequent steps, such as “preparation of pre-dispersion liquid”, “preparation of toothpaste base”, “paste making”, “fragrance addition and refining”, and “filling”, are the same as in Example 1, so they will not be described again.
[0139] The toothpaste samples prepared in Examples 1-5 and Comparative Examples 1-3 were subjected to the following performance tests.
[0140] 1. Physicochemical and stability tests
[0141] pH, paste appearance, and hard particle tests were conducted according to the national standard GB / T 8372-2017 "Toothpaste". Heat resistance tests (3 months at 45℃±1℃) and cold resistance tests (1 month at -18℃±2℃) were also performed to observe the paste stability. Foam volume was determined using the Roche-Myers foam analyzer method (40℃, 0.25% aqueous paste solution). The results are shown in Table 1.
[0142] Table 1. Physicochemical properties and stability test results of toothpaste samples
[0143] As shown in Table 1, all examples conform to national standards. Comparative Example 3 (sodium lauroyl alanine salt) exhibited abnormalities in both heat and cold resistance tests, and the initial paste had a grainy texture. The resulting sodium lauroyl alanine salt aqueous solution showed a slight opalescence at room temperature, which was due to the formation of high-concentration micelles, indicating that it easily forms micelle aggregates at higher concentrations, which is detrimental to paste stability. In contrast, the aqueous solution of lauroyl alanine arginine salt of this invention was clear and transparent, proving that the arginine salt structure effectively inhibited micelle formation. This verifies that the use of arginine salt in this invention can effectively solve micelle and dispersibility problems, and the stability meets the requirements for mass production. Comparative Example 1 (SLS) had the highest foaming volume but posed a risk of irritation.
[0144] 2. Oral mucosal irritation test
[0145] An in vitro cell model was used: human immortalized oral keratinocytes (HOK). Toothpaste from each group was diluted to a 0.1% solution with culture medium and co-cultured with cells for 24 hours. The relative cell proliferation rate was detected using the CCK-8 assay, and the IC50 value (half-maximal inhibitory concentration, with a higher value indicating milder inhibition) was calculated. Simultaneously, cell morphology was observed using a scoring method (0 points: no damage; 1 point: slight damage; 2 points: moderate damage; 3 points: severe damage). The results are shown in Table 2.
[0146] Table 2. Results of cell irritation test on toothpaste samples
[0147] As shown in Table 2, the toothpastes of Examples 1-5 of this invention are almost non-toxic to oral keratinocytes, with IC50 values greater than 1.0%, normal cell morphology, and are extremely mild. Comparative Example 1 (SLS) showed significant cytotoxicity, with an IC50 of only 0.18%. Although Comparative Example 3 is milder than SLS, it is still not as mild as this invention.
[0148] 3. Anti-inflammatory and mucosal repair efficacy test
[0149] Toothpastes from Examples 1, 3, 5, 2, and 3 were used for testing.
[0150] A gingival fibroblast (HGF-1) inflammation model was used: cells were stimulated with 1 μg / mL LPS (lipopolysaccharide) and 0.05% toothpaste sample solution was added simultaneously. After 24 hours of culture, the levels of inflammatory factors IL-6 and TNF-α in the supernatant were measured (ELISA). Simultaneously, a cell scratch assay was performed to evaluate the promoting effect of toothpaste on cell migration (simulating mucosal repair). During the test, a model control group was established, where cells were directly cultured after stimulation with 1 μg / mL LPS; a blank control group was established, where cells were stimulated with 1 μg / mL LPS and cultured with an equal volume of purified water. The results are shown in Table 3.
[0151] Table 3. Results of anti-inflammatory and repair-promoting tests on toothpaste samples
[0152] Table 3 shows that, compared with the model control group stimulated only by LPS, Examples 1, 3, and 5 significantly inhibited the secretion of inflammatory factors IL-6 and TNF-α (p<0.01) and greatly improved the cell scratch healing rate, demonstrating excellent anti-inflammatory and mucosal repair-promoting activities. Comparative Example 2 (fish-derived PDRN) had a much smaller molecular weight and its effect was far less than that of the examples. Comparative Example 3 (sodium salt) showed the worst effect, indicating a synergistic effect between arginine and PDRN.
[0153] 4. Dentin tubule sealing test
[0154] Toothpastes from Examples 1, 4, 1, 2 and 3 were used for testing.
[0155] Dentin sections were prepared from extracted human teeth, etched with acid, and then treated with toothpaste slurry (toothpaste:water = 1:3) for 5 minutes. After cleaning and drying, the sealing of dentinal tubules was observed using SEM, and the sealing rate was calculated. The results are shown in Table 4.
[0156] Table 4. Results of dentinal tubule sealing rate test
[0157] As shown in Table 4, the dentinal tubule sealing rates after treatment in Examples 1 and 4 both exceeded 85%, demonstrating the significant promoting effect of arginine and the mild system on remineralization in this invention. Comparative Example 1 (SLS) had almost no sealing effect, and Comparative Example 3 (sodium salt) had limited effect.
[0158] 5. Antibacterial performance test
[0159] The diameter of the inhibition zone of toothpaste against Porphyromonas gingivalis (ATCC 33277) and Streptococcus mutans (ATCC 25175) was determined by the agar diffusion method. The results are shown in Table 5.
[0160] Table 5. Results of inhibition zone diameter test (unit: mm)
[0161] As shown in Table 5, all samples exhibited some antibacterial activity. Example 5, due to the addition of zinc citrate, showed the best antibacterial effect. Overall, the examples of this invention are superior to Comparative Example 3 (sodium salt).
[0162] The above examples, comparative examples, and performance test data fully demonstrate that the PDRN-amino acid composite toothpaste provided by this invention, due to its specific component selection (spirulina PDRN, glycine, lauroyl alanine arginine salt and APG compound) and preparation method, brings unexpected technical effects such as mild and non-irritating properties, highly effective anti-inflammatory repair, stable paste, and rich foam, and has significant creative and practical value.
[0163] The above embodiments are merely illustrative of the concept and technical solution of the present invention and are not intended to limit the present invention. Any person skilled in the art can modify or alter the above embodiments without departing from the spirit and scope of the present invention. Therefore, all equivalent modifications or alterations made by those skilled in the art without departing from the spirit and technical concept disclosed in the present invention should still be covered by the claims of the present invention.
[0164] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.
Claims
1. A PDRN-amino acid complex toothpaste, characterized in that, The product comprises, by weight percentage: 20%–60% humectant, 15%–25% abrasive, 1%–4% foaming agent combination, 0.1%–2% sweetener, 0.5%–1.5% thickener, 0.8%–1.8% fragrance, 0.1%–0.5% preservative, 0.1%–1.5% main active ingredient, 0.1%–3% auxiliary active ingredient, and the balance being water; wherein the main active ingredient comprises glycine and polydeoxyribonucleotides derived from spirulina; and the foaming agent combination comprises lauroyl alanine arginine salt and alkyl glycoside, wherein the weight ratio of lauroyl alanine arginine salt to the alkyl glycoside is 1:3 to 3:
1.
2. The toothpaste containing PDRN-amino acid complex according to claim 1, characterized in that, The length of the polydeoxyribonucleotide fragments derived from spirulina is 50-100 bp.
3. The toothpaste containing PDRN-amino acid complex according to claim 1, characterized in that, In the foaming agent combination, the weight ratio of lauroyl alanine arginine salt to alkyl glycoside is 1:
1.
4. The toothpaste containing PDRN-amino acid complex according to claim 1, characterized in that, The lauroyl alanine arginine salt is prepared by neutralizing lauroyl alanine and L-arginine in water. The resulting aqueous solution of lauroyl alanine arginine salt has a mass fraction of 20 wt% and a pH of 6.30~7.
30.
5. The toothpaste containing PDRN-amino acid complex according to claim 1, characterized in that, The auxiliary functional ingredients are selected from one or more of zinc citrate, sodium phytate, tetrasodium pyrophosphate, sodium monofluorophosphate, and sodium fluoride; the moisturizer is selected from one or more of sorbitol, glycerin, propylene glycol, and polyethylene glycol; the abrasive is selected from one or more of hydrated silica, calcium hydrogen phosphate dihydrate, and anhydrous calcium hydrogen phosphate; the sweetener is selected from one or more of sucralose, trehalose, sodium saccharin, and dipotassium glycyrrhizate; the thickener is selected from one or more of xanthan gum, cellulose gum, and hydroxypropyl guar gum; and the preservative is selected from one or more of methylparaben, sodium benzoate, and potassium sorbate.
6. A method for preparing a toothpaste containing a PDRN-amino acid complex according to any one of claims 1 to 5, characterized in that, Includes the following steps: (1) Preparation of lauroyl alanine arginine salt aqueous solution: Lauroyl alanine and L-arginine were neutralized in water to prepare lauroyl alanine arginine salt aqueous solution; (2) Preparation of pre-dissolved solution 1: Dissolve the sweetener, main active ingredient, preservative and water-soluble auxiliary active ingredient in a portion of water, heat and stir until completely dissolved to obtain pre-dissolved solution 1; (3) Preparation of pre-dissolving solution 2: Mix and dissolve the first part of the moisturizer, the lauroyl alanine arginine salt aqueous solution obtained in step (1), the alkyl glycoside and the remaining water to obtain pre-dissolving solution 2; (4) Preparation of pre-dispersion: Add the thickener to the second part of the humectant, stir and disperse to obtain the pre-dispersion; (5) Preparation of toothpaste base: Mix pre-dissolving solution 1, pre-dissolving solution 2 and pre-dispersing solution, let stand to swell, and obtain toothpaste base; (6) Mix the toothpaste gel base with the abrasive and homogenize; (7) Add fragrance and continue homogenization. After defoaming, the finished paste is obtained.
7. The method for preparing the PDRN-amino acid complex toothpaste according to claim 6, characterized in that, In step (3), the first part of the moisturizer accounts for 60% to 80% of the total moisturizer mass; in step (4), the second part of the moisturizer accounts for 20% to 40% of the total moisturizer mass.
8. The method for preparing the PDRN-amino acid complex toothpaste according to claim 6, characterized in that, In step (1), the neutralization reaction temperature is 60℃~70℃, and it is supplemented by ultrasonic treatment for 10min~20min, with ultrasonic power of 150W~250W; in step (2), the heating and stirring is carried out at 40℃~65℃ at 800rpm~1000rpm for 10min~25min; in step (3), the mixing and dissolving is carried out at room temperature at 800rpm~1000rpm for 8min~15min; in step (4), the stirring and dispersing is carried out at room temperature at 800rpm~1000rpm for 2min~8min.
9. The method for preparing the PDRN-amino acid complex toothpaste according to claim 6, characterized in that, In step (5), the mixing is carried out by stirring at 800 rpm to 1000 rpm for 20 min to 40 min, and the standing swelling time is more than 8 hours.
10. The method for preparing the PDRN-amino acid complex toothpaste according to claim 6, characterized in that, In steps (6) and (7), the stirring speed for homogenization is 800 rpm to 1200 rpm, and the homogenization time is 4 min to 18 min.