Anti-wrinkle essence containing Panax notoginseng oil and its preparation method

Abstract

This invention belongs to the field of cosmetic technology and discloses an anti-wrinkle essence containing Panax notoginseng oil and its preparation method. The essence comprises Panax notoginseng oil, phospholipids, a membrane flexibility regulating component, tocopheryl acetate, a polyol moisturizer, xanthan gum, carbomer, a pH adjuster, a preservative system, and water. The Panax notoginseng oil, phospholipids, the membrane flexibility regulating component, and tocopheryl acetate together constitute a flexible nanoliposome dispersion system. Panax notoginseng oil, as a functional oil phase, participates in the lipid bilayer construction and is dispersed in the aqueous essence system in a structured encapsulation form. Tocopheryl acetate exists in the flexible nanoliposomes as a membrane stabilizing component and an oil phase antioxidant protective component. The preparation method includes preparing the aqueous phase of the essence matrix, preparing a lipid phase premix containing Panax notoginseng oil, dropwise adding it to form an initial dispersion and homogenizing under high pressure, vacuum degassing, and adjusting the pH to obtain the finished product. This essence exhibits good dispersion stability, strong antioxidant capacity, and good anti-wrinkle effects.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to an anti-wrinkle essence containing Panax notoginseng oil and its preparation method. Background Technology

[0002] As we age, the amount of collagen, elastin, and natural moisturizing factors in our skin gradually decreases, and the stratum corneum barrier function declines, leading to signs of aging such as sagging, fine lines, and wrinkles. To address these issues, a large number of anti-wrinkle cosmetics have appeared on the market. One type of product primarily uses polymeric film-forming agents to create a temporary tightening film on the skin's surface, resulting in an immediate firming effect. However, this effect is mostly superficial and lacks staying power, easily diminishing after washing, making it difficult to achieve long-term improvement in skin condition. Another type of product improves skin appearance by adding plant extracts, oil-based active ingredients, or moisturizing components. However, the poor dispersibility, tendency to aggregate and separate, and insufficient stability of some oil-soluble active ingredients in aqueous serum systems limit their effectiveness.

[0003] Panax notoginseng, a traditional plant resource, contains various bioactive components, among which Panax notoginseng oil has certain potential applications in skin care. However, when Panax notoginseng oil is used directly in serum systems, it is easily oxidized by light, heat, and oxygen, resulting in off-odors, color changes, and decreased activity. It also suffers from uneven dispersion and poor storage stability in aqueous systems. In existing technologies, conventional emulsification methods struggle to achieve a refreshing feel, dispersion stability, and sustained anti-wrinkle effects. Therefore, developing an anti-wrinkle serum and its preparation method that can improve the stability of Panax notoginseng oil, enhance its dispersion in serum systems, and improve its skin delivery performance has practical application value. Summary of the Invention

[0004] To address the shortcomings mentioned in the background art, the present invention aims to provide an anti-wrinkle essence containing Panax notoginseng oil and its preparation method. This essence uses Panax notoginseng oil as the functional oil phase, and combines it with phospholipids, membrane flexibility regulating components, and tocopheryl acetate to construct a flexible nanoliposome dispersion system, allowing Panax notoginseng oil to be stably dispersed in the aqueous essence in a structured encapsulated form. Tocopheryl acetate provides both membrane stabilization and antioxidant protection to the oil phase. The preparation method employs light-protected, inert atmosphere, low-temperature premixing, dropwise dispersion, high-pressure homogenization, and vacuum degassing processes. This invention can improve the dispersion uniformity, storage stability, and anti-wrinkle efficacy of Panax notoginseng oil.

[0005] The objective of this invention can be achieved through the following technical solutions: An anti-wrinkle essence containing Panax notoginseng oil, comprising the following components by weight: 0.5-3 parts Panax notoginseng oil, 5-30 parts phospholipids, 1-8 parts membrane flexibility regulating component, 0.5-2 parts tocopheryl acetate, 50-120 parts polyol moisturizer, 0.3-1.5 parts xanthan gum, 0.8-2.5 parts carbomer, 0.5-2.5 parts pH adjuster, 6-12 parts preservative system, and water as balance; The Panax notoginseng oil, phospholipids, membrane flexibility regulating components, and tocopheryl acetate together constitute a flexible nanoliposome dispersion system. The Panax notoginseng oil participates in the lipid bilayer construction as a functional oil phase and is dispersed in the aqueous essence system in a structured encapsulation form. The tocopheryl acetate exists in the flexible nanoliposomes as a membrane stabilizing component and an oil phase antioxidant protective component. The average particle size of the flexible nanoliposomes is 60-140 nm, the PDI is not greater than 0.30, and the encapsulation efficiency is not less than 70%.

[0006] More preferably, the phospholipid is selected from one or more of soybean phospholipids, lecithin, hydrogenated lecithin, and phosphatidylcholine.

[0007] More preferably, the membrane flexibility regulating component is selected from one or two of sodium cholate and sodium deoxycholate.

[0008] More preferably, the weight ratio of Panax notoginseng oil to phospholipid is 1:10 to 1:16; and the weight ratio of tocopherol acetate to Panax notoginseng oil is 0.3:1 to 1.2:1.

[0009] More preferably, the polyol humectant is selected from one or more of glycerin, butylene glycol, 1,3-propanediol, and methylpropanediol; the pH adjuster is selected from one or more of arginine, aminomethylpropanol, and tromethamine; and the preservative system is selected from one or more of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, and ethylhexylglycerin.

[0010] More preferably, the anti-wrinkle essence further includes 0.5 to 5 parts of emulsifying and matrix stabilizing components, wherein the emulsifying and matrix stabilizing components are selected from one or two of cetearyl glucoside, fatty alcohol polyoxyethylene ether, and glyceryl monostearate.

[0011] More preferably, the xanthan gum and carbomer together constitute an essence matrix network, the flexible nanoliposomes are dispersed in the essence matrix network, and the pH of the essence is 5.5 to 6.5.

[0012] A method for preparing an anti-wrinkle serum includes the following steps: S1. Mix a portion of water with a polyol moisturizer and a membrane flexibility regulating component to obtain the liposome hydrated phase; mix the remaining water with xanthan gum, carbomer, emulsifying and matrix stabilizing components and a pH adjuster to obtain the essence matrix aqueous phase; S2. Under light-proof, inert atmosphere and low temperature conditions, phospholipids, tocopheryl acetate and Panax notoginseng oil are mixed to obtain a lipid-phase premix containing Panax notoginseng oil. S3. The lipid premix containing Panax notoginseng oil is slowly added dropwise to the hydration phase of the liposomes to form a preliminary dispersion under stirring conditions. The preliminary dispersion is then subjected to high-pressure homogenization to obtain a flexible nanoliposome dispersion. S4. The flexible nanoliposome dispersion is incorporated into the aqueous phase of the essence matrix, vacuum degassing is performed, a preservative system is added, the pH is adjusted, and the mixture is stirred evenly to obtain the essence with anti-wrinkle effects.

[0013] More preferably, the preparation temperature of the lipid phase premix in step S2 is 30-45°C, and the lipid phase premix containing Panax notoginseng oil in step S3 is added to the liposome hydration phase by slow dropwise addition. The high-pressure homogenization pressure is 600-1000 bar, the number of cycles is 3-5, and the material temperature during the homogenization process does not exceed 35°C.

[0014] More preferably, nitrogen protection is used in step S2, the vacuum degassing degree in step S4 is -0.06 to -0.09 MPa, the degassing time is 5 to 20 min, the flexible nanoliposome dispersion is incorporated into the aqueous phase of the essence matrix at a temperature not exceeding 30°C, the preservative system is added at a temperature not exceeding 30°C, the pH of the resulting essence is adjusted to 5.5 to 6.5, and the stirring speed is 300 to 800 rpm.

[0015] The beneficial effects of this invention are: This invention constructs a flexible nanoliposome dispersion system by co-constructing Panax notoginseng oil, phospholipids, membrane flexibility regulating components, and tocopheryl acetate. This system transforms Panax notoginseng oil from its ordinary free droplet form within the serum into a structured, stably dispersed form within an aqueous matrix, significantly improving the dispersion uniformity, system appearance stability, and storage stability of the Panax notoginseng oil in the formulation. Since Panax notoginseng oil participates in the lipid bilayer construction as a functional oil phase, it enhances the spreadability and local delivery performance on the skin surface while maintaining the serum's refreshing feel. The flexible nanoliposome structure facilitates the compatibility of active ingredients with the lipid environment of the stratum corneum, enhancing the effective utilization of Panax notoginseng oil on the skin surface. Simultaneously, tocopheryl acetate, acting as a membrane stabilizing component and an antioxidant protective component in the oil phase, is distributed within the lipid phase or lipid bilayer, effectively reducing the risk of membrane oxidation, mitigating liposome membrane structure damage and content leakage, and further improving the chemical, physical, and encapsulation stability of the Panax notoginseng oil flexible nanoliposome system. By combining light-shielding, inert atmosphere, low-temperature premixing, dropwise dispersion, high-pressure homogenization, and vacuum degassing preparation processes, this invention can reduce the oxidative degradation of Panax notoginseng oil during preparation and storage, and avoid the activity loss and stratification problems caused by traditional high-temperature or crude emulsification methods. Detailed Implementation

[0016] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0017] In the following examples and comparative examples, the phospholipid is soybean phospholipid, the membrane flexibility regulating component is sodium deoxycholate, the polyol humectant is glycerol and 1,3-propanediol, the pH regulator is arginine, the preservative system is 1,2-hexanediol and p-hydroxyacetophenone, and the emulsifying and matrix stabilizing component is cetearyl glucoside.

[0018] Example 1: Verification that under conditions of low activity and low excipient dosage, the present invention can still form a stable essence system and has basic ability to construct flexible nanoliposomes and feasibility for anti-wrinkle applications.

[0019] An anti-wrinkle essence containing Panax notoginseng oil, comprising the following components by weight: 0.5 parts Panax notoginseng oil, 5 parts phospholipids, 1 part membrane flexibility regulating component, 0.5 parts tocopheryl acetate, 50 parts polyol moisturizer, 0.3 parts xanthan gum, 0.8 parts carbomer, 0.5 parts pH adjuster, 6 parts preservative system, and water balance. The preparation steps are as follows: S1. Weigh 500.0g of purified water and add it to a light-protected container. Add 25.0g of glycerol, 25.0g of 1,3-propanediol, and 1.0g of sodium deoxycholate. Stir at 500rpm for 15min at 30℃ to obtain the liposome hydrated phase. Separately weigh 330.0g of purified water and add it to another mixing container. Stir at 500rpm at room temperature. Add 0.3g of xanthan gum and 0.8g of carbomer sequentially, and stir to disperse for 30min. Then add 0.5g of cetearyl glucoside to 50.0g of purified water at 70℃, stir for 15min, cool to below 30℃, and then add it to the xanthan gum and carbomer dispersion system to obtain the aqueous phase of the essence matrix. Separately, dissolve 0.5g of arginine in 20.0g of purified water to prepare a pH adjustment solution for later use.

[0020] S2. In another light-proof container, nitrogen gas is introduced for protection. 5.0g of soybean lecithin and 0.5g of tocopheryl acetate are added and stirred at 300rpm for 20min at 40℃. Then 0.5g of Panax notoginseng oil is added and stirred for another 20min at 35-40℃ to obtain a lipid premix containing Panax notoginseng oil.

[0021] S3. The lipid premix containing Panax notoginseng oil is slowly added dropwise to the hydrated phase of liposomes at 30°C for 20 minutes, with a stirring speed of 600 rpm during the addition. After the addition is complete, stirring is continued for 20 minutes to form a preliminary dispersion. The preliminary dispersion is then fed into a high-pressure homogenizer and homogenized three times at 600 bar, with the material temperature controlled not to exceed 35°C during homogenization, to obtain a flexible nanoliposome dispersion.

[0022] S4. The obtained flexible nanoliposome dispersion was slowly added to the aqueous phase of the essence matrix at a temperature below 30°C and stirred at 300 rpm for 15 min. 3.0 g of 1,2-hexanediol and 3.0 g of p-hydroxyacetophenone were added and stirred for another 10 min. Then, an aqueous solution of arginine was added to adjust the pH of the system to 5.5. Subsequently, the system was degassed under vacuum at -0.06 MPa for 5 min. Finally, purified water was added to make up to 1000 g and stirred evenly to obtain the essence containing Panax notoginseng oil with anti-wrinkle effects.

[0023] Example 2: The film-forming carrier construction, formulation carrying capacity and process adaptability of the system of the present invention under a higher feed level were investigated to verify its scale-up application and high-load implementation effect.

[0024] An anti-wrinkle essence containing Panax notoginseng oil, comprising the following components by weight: 3 parts Panax notoginseng oil, 30 parts phospholipids, 8 parts membrane flexibility regulating component, 2 parts tocopheryl acetate, 120 parts polyol moisturizer, 1.5 parts xanthan gum, 2.5 parts carbomer, 2.5 parts pH adjuster, 12 parts preservative system, and water balance. The preparation steps for the anti-wrinkle essence containing Panax notoginseng oil are as follows: S1. Under light-protected conditions, 60.0 g glycerol, 60.0 g 1,3-propanediol, and 8.0 g sodium deoxycholate were added to 400.0 g purified water and stirred at 600 rpm for 20 min at 35 °C to obtain the liposome hydrated phase. At room temperature, 1.5 g xanthan gum and 2.5 g carbomer were added to 300.0 g purified water in another stirred container and stirred at 600 rpm for 40 min to fully wet the liposomes and form a homogeneous matrix dispersion. Then, 5.0 g cetearyl glucoside was added to 50.0 g purified water heated to 75 °C and stirred for 15 min. After cooling to below 30 °C, it was added to the above matrix dispersion and stirred for another 15 min to obtain the aqueous phase of the essence matrix. Finally, 2.5 g arginine was dissolved in 20.0 g purified water for later use.

[0025] S2. Nitrogen gas is introduced into another light-proof container, followed by the addition of 30.0g soybean lecithin and 2.0g tocopheryl acetate. The mixture is stirred at 45℃ and 400rpm for 25min. After the system is evenly mixed, 3.0g Panax notoginseng oil is added, and the mixture is stirred for another 25min at 40-45℃ to obtain a lipid premix containing Panax notoginseng oil.

[0026] S3. The lipid premix is ​​slowly added dropwise to the liposome hydrated phase at 30°C for 30 minutes, while the stirring speed is 800 rpm. After the addition is completed, stirring is continued for 25 minutes to obtain a preliminary dispersion. The preliminary dispersion is then introduced into a high-pressure homogenizer and homogenized five times continuously at 1000 bar, with the material temperature controlled below 35°C throughout the homogenization process, to finally obtain a flexible nanoliposome dispersion.

[0027] S4. The obtained flexible nanoliposome dispersion is slowly added to the aqueous phase of the essence matrix prepared in step S1 at a temperature below 30°C, and stirred at 800 rpm for 20 min. Then, 6.0 g of 1,2-hexanediol and 6.0 g of p-hydroxyacetophenone are added, and stirring is continued for 10 min. A pre-prepared arginine aqueous solution is then added to adjust the pH of the system to 6.5. Vacuum degassing is then performed at -0.09 MPa for 20 min. Finally, purified water is added to a total mass of 1000 g, and the mixture is stirred until homogeneous to obtain the essence containing Panax notoginseng oil with anti-wrinkle effects.

[0028] Example 3: Demonstrates the preferred implementation state of the present invention under the coordination of the proportions of each component and the preparation conditions.

[0029] An anti-wrinkle essence containing Panax notoginseng oil, comprising the following components by weight: 1.75 parts Panax notoginseng oil, 17.5 parts phospholipids, 4.5 parts membrane flexibility regulating component, 1.25 parts tocopheryl acetate, 85 parts polyol moisturizer, 0.9 parts xanthan gum, 1.65 parts carbomer, 1.5 parts pH adjuster, 9 parts preservative system, and water balance. The preparation steps for the anti-wrinkle essence containing Panax notoginseng oil are as follows: S1. Add 450.0g of purified water to a light-protected container, then add 42.5g of glycerol, 42.5g of 1,3-propanediol, and 4.5g of sodium deoxycholate. Stir at 550rpm for 18min at 33℃ to obtain the liposome hydrated phase. Separately, add 330.0g of purified water to another container, and add 0.9g of xanthan gum and 1.65g of carbomer sequentially under stirring at room temperature. Continue stirring for 35min to obtain the matrix dispersion system. Next, add 2.75g of cetearyl glucoside to 50.0g of purified water heated to 72℃ and stir for 15min. After cooling to below 30℃, add it to the above matrix dispersion system and continue stirring for 15min to obtain the essence matrix aqueous phase. Then, dissolve 1.5g of arginine in 20.0g of purified water to prepare a pH-adjusting solution for later use.

[0030] S2. Under light-protected conditions, nitrogen gas was introduced into another container, followed by the addition of 17.5g of soybean lecithin and 1.25g of tocopheryl acetate. The mixture was stirred at 37°C and 350rpm for 22 minutes. After the system was mixed evenly, 1.75g ​​of Panax notoginseng oil was added, and the mixture was stirred at around 37°C for another 22 minutes to obtain a lipid premix containing Panax notoginseng oil.

[0031] S3. The lipid premix was slowly added dropwise to the liposome hydrated phase at 30°C over a period of 25 minutes, with stirring maintained at 550 rpm during the addition. After the addition was complete, stirring was continued for another 20 minutes to obtain a preliminary dispersion. The preliminary dispersion was then subjected to high-pressure homogenization at a pressure of 800 bar, with homogenization performed four times. The material temperature was controlled to not exceed 35°C during the homogenization process to obtain a flexible nanoliposome dispersion.

[0032] S4. Slowly add the flexible nanoliposome dispersion to the aqueous phase of the essence matrix at below 30℃, and stir at 550 rpm for 15 min. Then add 4.5 g of 1,2-hexanediol and 4.5 g of p-hydroxyacetophenone, and continue stirring for 10 min. Then add the pre-prepared arginine aqueous solution to adjust the pH of the system to 6.0. After pH adjustment, perform vacuum degassing at -0.075 MPa for 12.5 min. Finally, add purified water to a total mass of 1000 g, stir evenly, and obtain the essence containing Panax notoginseng oil with anti-wrinkle effects.

[0033] Comparative Example 1: This comparative example does not include the membrane flexibility regulating component sodium deoxycholate. The types, amounts, and preparation steps of other raw materials are kept the same as in Example 3 to verify the effect of the membrane flexibility regulating component on the formation of flexible nanoliposomes, system stability, and anti-wrinkle effects.

[0034] An anti-wrinkle essence containing Panax notoginseng oil, comprising the following components by weight: 1.75 parts Panax notoginseng oil, 17.5 parts phospholipids, 1.25 parts tocopheryl acetate, 85 parts polyol moisturizer, 0.9 parts xanthan gum, 1.65 parts carbomer, 1.5 parts pH adjuster, 9 parts preservative system, and water balance. The preparation steps for the anti-wrinkle essence containing Panax notoginseng oil are as follows: S1. Add 450.0g of purified water to a light-protected container, then add 42.5g of glycerol and 42.5g of 1,3-propanediol. Stir at 550rpm for 18min at 33℃ to obtain the comparative liposome hydrated phase. Separately, add 330.0g of purified water to another container, stir at room temperature, and then add 0.9g of xanthan gum and 1.65g of carbomer sequentially, ensuring thorough dispersion. Continue stirring for 35min to obtain the matrix dispersion system. Next, add 2.75g of cetearyl glucoside to 50.0g of purified water heated to 72℃, stir for 15min, and after cooling to below 30℃, add it to the above matrix dispersion system. Continue stirring for 15min to obtain the essence matrix aqueous phase. Then, dissolve 1.5g of arginine in 20.0g of purified water to prepare a pH-adjusting solution for later use.

[0035] S2. Under light-protected conditions, nitrogen gas was introduced into another container, followed by the addition of 17.5g of soybean lecithin and 1.25g of tocopheryl acetate. The mixture was stirred at 37°C and 350rpm for 22 minutes. After the system was mixed evenly, 1.75g ​​of Panax notoginseng oil was added, and the mixture was stirred at around 37°C for another 22 minutes to obtain a lipid premix containing Panax notoginseng oil.

[0036] S3. The lipid premix was slowly added dropwise to the liposome hydrated phase at 30°C for 25 minutes, with stirring maintained at 550 rpm during the addition. After the addition was complete, stirring was continued for 20 minutes to form a preliminary dispersion. The preliminary dispersion was then subjected to high-pressure homogenization at 800 bar for 4 cycles, with the material temperature controlled to not exceed 35°C during homogenization, to obtain a lipid nano-dispersion.

[0037] S4. Slowly add the lipid nano-dispersion to the aqueous phase of the essence matrix at below 30℃ and stir at 550 rpm for 15 min. Then add 4.5 g of 1,2-hexanediol and 4.5 g of p-hydroxyacetophenone, and continue stirring for 10 min. Then add the pre-prepared arginine aqueous solution to adjust the pH of the system to 6.0. After pH adjustment, perform vacuum degassing at -0.075 MPa for 12.5 min. Finally, add purified water to a total mass of 1000 g, stir evenly, and the essence product of Comparative Example 1 is obtained.

[0038] Comparative Example 2: This comparative example does not include tocopherol acetate. The types, amounts, and preparation steps of other raw materials are the same as in Example 3 to verify the effect of tocopherol acetate as a membrane stabilizing component and an oil phase antioxidant protective component on the system stability and anti-wrinkle effects.

[0039] An anti-wrinkle essence containing Panax notoginseng oil, comprising the following components by weight: 1.75 parts Panax notoginseng oil, 17.5 parts phospholipids, 4.5 parts membrane flexibility regulating component, 85 parts polyol moisturizer, 0.9 parts xanthan gum, 1.65 parts carbomer, 1.5 parts pH adjuster, 9 parts preservative system, and water balance. The preparation steps for the anti-wrinkle essence containing Panax notoginseng oil are as follows: S1. First, add 450.0g of purified water to a light-protected container, then add 42.5g of glycerol, 42.5g of 1,3-propanediol, and 4.5g of sodium deoxycholate. Stir at 550rpm for 18min at 33℃ to obtain the liposome hydrated phase. Separately, add 330.0g of purified water to another container, stir at room temperature, and then add 0.9g of xanthan gum and 1.65g of carbomer sequentially, ensuring thorough dispersion. Continue stirring for 35min to obtain the matrix dispersion system. Next, add 2.75g of cetearyl glucoside to 50.0g of purified water heated to 72℃, stir for 15min, and after cooling to below 30℃, add it to the above matrix dispersion system. Continue stirring for 15min to obtain the essence matrix aqueous phase. Then, dissolve 1.5g of arginine in 20.0g of purified water to prepare a pH adjustment solution for later use.

[0040] S2. Under light-protected conditions, nitrogen gas was introduced into another container, followed by the addition of 17.5g of soybean lecithin. The mixture was stirred at 37°C and 350rpm for 22 minutes. After the system was mixed evenly, 1.75g ​​of Panax notoginseng oil was added, and the mixture was stirred at around 37°C for another 22 minutes to obtain a lipid premix containing Panax notoginseng oil.

[0041] S3. The lipid premix was slowly added dropwise to the liposome hydrated phase at 30°C for 25 minutes, with stirring maintained at 550 rpm during the addition. After the addition was complete, stirring was continued for 20 minutes to form a preliminary dispersion. The preliminary dispersion was then subjected to high-pressure homogenization at 800 bar for 4 cycles, with the material temperature controlled not to exceed 35°C during homogenization, thus obtaining a lipid nano-dispersion.

[0042] S4. Slowly add the lipid nano-dispersion to the aqueous phase of the essence matrix at below 30℃ and stir at 550 rpm for 15 min. Then add 4.5 g of 1,2-hexanediol and 4.5 g of p-hydroxyacetophenone, and continue stirring for 10 min. Next, add the pre-prepared arginine aqueous solution to adjust the pH of the system to 6.0. After pH adjustment, perform vacuum degassing at -0.075 MPa for 12.5 min. Finally, add purified water to a total mass of 1000 g and stir evenly to obtain the essence product of Comparative Example 2.

[0043] Performance testing: 1. Characterization of flexible nanoliposomes A suitable amount of freshly prepared sample was diluted with purified water, and the average particle size and PDI were determined using a dynamic light scattering instrument. The Zeta potential was determined by electrophoretic light scattering. Another sample was subjected to ultrafiltration and centrifugation to separate free Panax notoginseng oil. The total and free Panax notoginseng oil contents in the sample were determined by high-performance liquid chromatography (HPLC), and the encapsulation efficiency was calculated. Each sample was measured in triplicate, and the results are expressed as averages. The results are shown in Table 1 below.

[0044] Table 1 Structural characterization results

[0045] As shown in Table 1, Examples 1-3 all formed flexible nanoliposome systems with small particle size, narrow distribution, and high encapsulation efficiency, indicating that the component design and preparation process adopted in this invention can effectively achieve nano-encapsulation of Panax notoginseng oil. Among them, Example 3 showed a more balanced performance in terms of particle size, PDI, Zeta potential, and encapsulation efficiency, demonstrating a superior overall molding effect. In Comparative Example 1, after removing the membrane flexibility adjustment component, the particle size increased, the PDI increased, and the encapsulation efficiency decreased significantly, indicating that this component plays an important role in the formation and dispersion stability of flexible nanoliposomes. In Comparative Example 2, without the addition of tocopherol acetate, the absolute value of the Zeta potential decreased and the encapsulation efficiency decreased, indicating that tocopherol acetate helps to improve the structural stability and encapsulation capacity of the system.

[0046] 2. Stability Test Take appropriate amounts of each sample, seal them separately, and place them in a 45℃ constant temperature incubator for accelerated stability testing for 30 days. Samples were taken at 0 days and 30 days to observe appearance changes, and the peroxide value and encapsulation efficiency were measured. The peroxide value was determined using the oil peroxide value determination method. The encapsulation efficiency was achieved by separating free Panax notoginseng oil using ultrafiltration centrifugation, followed by high performance liquid chromatography (HPLC) to determine the total and free Panax notoginseng oil content, and the encapsulation efficiency and encapsulation efficiency retention rate were calculated. Each sample was measured in triplicate, and the results were averaged. The results are shown in Table 2 below.

[0047] Table 2 Stability Test Results

[0048] As shown in Table 2, Examples 1-3 maintained low peroxide values ​​and high encapsulation retention rates under accelerated conditions at 45°C, and exhibited stable appearance without significant stratification, indicating that the system of the present invention possesses good chemical and structural stability. In contrast, after removing the membrane flexibility adjustment component in Comparative Example 1, the encapsulation retention rate significantly decreased and slight stratification occurred; after removing tocopherol acetate in Comparative Example 2, the peroxide value significantly increased and a slight oily odor appeared, indicating that tocopherol acetate plays an important role in inhibiting oxidation and maintaining system stability.

[0049] 3. Skin retention and delivery test Ex vivo porcine abdominal skin was harvested, subcutaneous fat and fascia were removed, and the skin was fixed in a Franz vertical diffusion cell with the stratum corneum facing the donor chamber. The recipient solution was phosphate-buffered saline (PBS):ethanol = 70:30 (V / V), kept at a constant temperature (32±0.5℃), and stirred at 300 rpm. An equal volume of sample was added to the donor chamber, sealed, and protected from light for 24 hours. After the experiment, the skin was removed, and residual sample was gently wiped away with physiological saline. The skin was then minced, and vortexed and ultrasonically extracted with anhydrous ethanol. The supernatant was collected by centrifugation, and the amount of Panax notoginseng oil retained in the skin was determined by high-performance liquid chromatography (HPLC). Simultaneously, the recipient solution was collected, and the cumulative permeation over 24 hours was measured. Each group was measured in triplicate, and the average value was taken. The results are shown in Table 3 below.

[0050] Table 3. Results of skin retention and in vitro transdermal testing

[0051] As shown in Table 3, the skin retention and 24-hour cumulative permeation of Examples 1-3 were significantly higher than those of Comparative Examples 1 and 2, indicating that the flexible nanoliposome system constructed in this invention can effectively improve the retention and delivery capacity of Panax notoginseng oil on the skin surface and in the stratum corneum. In Comparative Example 1, after removing the membrane flexibility regulating component, both skin retention and permeation decreased significantly, indicating that this component plays an important role in improving carrier flexibility and skin delivery capacity. Similarly, in Comparative Example 2, after removing tocopheryl acetate, the delivery performance also decreased, indicating that it has a positive effect on maintaining system stability and promoting effective delivery.

[0052] 4. Human efficacy test Fifty healthy female subjects aged 35-55 years were recruited and randomly divided into 5 groups of 10 each. Each group used the corresponding sample for 28 consecutive days, twice daily (morning and evening), applying it evenly to a fixed test area on the face. Wrinkle depth, wrinkle area, and wrinkle volume were measured using a skin 3D image analyzer before and after 28 days of use; skin roughness Ra was measured using a skin roughness meter; skin elasticity parameters were measured using a skin elasticity meter; skin moisture content was measured using a stratum corneum moisture meter; and transepidermal water loss (TEWL) was measured using a transepidermal water loss meter. All indicators are expressed as the rate of change relative to baseline values, and the results for each group are expressed as averages. The results are shown in Table 4 below.

[0053] Table 4 Results of Human Efficacy Tests

[0054] As shown in Table 4, after 28 days of continuous use, Examples 1-3 all significantly improved wrinkle depth, wrinkle area, wrinkle volume, and skin roughness, while also increasing skin elasticity and stratum corneum moisture content, and reducing transepidermal water loss. This indicates that the essence described in this invention not only has a good anti-wrinkle effect but also improves skin barrier function and overall skin condition. In contrast, although Comparative Examples 1 and 2 also showed some improvement, the improvement in various indicators was significantly lower than that of the Example groups, especially in wrinkle improvement, elasticity enhancement, and TEWL reduction. This suggests that the membrane flexibility regulating component and tocopheryl acetate play an important role in improving system stability, enhancing activity delivery, and ultimately achieving anti-wrinkle efficacy.

[0055] In the description of this specification, references to terms such as "an embodiment," "example," "specific example," etc., indicate that a specific feature, structure, material, or characteristic described in connection with that embodiment or example is included in at least one embodiment or example of the invention. In this specification, illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0056] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of the invention. Various changes and modifications can be made to the invention without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed invention.

Claims

1. An essence containing Panax notoginseng oil with anti-wrinkle effects, characterized in that, By weight, it includes the following components: 0.5-3 parts Panax notoginseng oil, 5-30 parts phospholipids, 1-8 parts membrane flexibility regulating component, 0.5-2 parts tocopheryl acetate, 50-120 parts polyol humectant, 0.3-1.5 parts xanthan gum, 0.8-2.5 parts carbomer, 0.5-2.5 parts pH adjuster, 6-12 parts preservative system, and water as balance; The Panax notoginseng oil, phospholipids, membrane flexibility regulating components, and tocopheryl acetate together constitute a flexible nanoliposome dispersion system. The Panax notoginseng oil participates in the lipid bilayer construction as a functional oil phase and is dispersed in the aqueous essence system in a structured encapsulation form. The tocopheryl acetate exists in the flexible nanoliposomes as a membrane stabilizing component and an oil phase antioxidant protective component. The average particle size of the flexible nanoliposomes is 60-140 nm, the PDI is not greater than 0.30, and the encapsulation efficiency is not less than 70%.

2. The anti-wrinkle essence according to claim 1, characterized in that, The phospholipid is selected from one or more of soybean phospholipids, lecithin, hydrogenated lecithin, and phosphatidylcholine.

3. The anti-wrinkle essence according to claim 1, characterized in that, The membrane flexibility regulating component is selected from one or both of sodium cholate and sodium deoxycholate.

4. The anti-wrinkle essence according to claim 1, characterized in that, The weight ratio of Panax notoginseng oil to phospholipids is 1:10 to 1:16; the weight ratio of tocopherol acetate to Panax notoginseng oil is 0.3:1 to 1.2:

1.

5. The anti-wrinkle essence according to claim 1, characterized in that, The polyol humectant is selected from one or more of glycerin, butylene glycol, 1,3-propanediol, and methylpropanediol; the pH adjuster is selected from one or more of arginine, aminomethylpropanol, and tromethamine; and the preservative system is selected from one or more of 1,2-hexanediol, 1,2-pentanediol, p-hydroxyacetophenone, phenoxyethanol, and ethylhexylglycerin.

6. The anti-wrinkle essence according to claim 1, characterized in that, It also includes 0.5 to 5 parts of emulsifying and matrix stabilizing components, wherein the emulsifying and matrix stabilizing components are selected from one or two of cetearyl glucoside, fatty alcohol polyoxyethylene ether, and glyceryl monostearate.

7. The anti-wrinkle essence according to claim 1, characterized in that, The xanthan gum and carbomer together form an essence matrix network, the flexible nanoliposomes are dispersed in the essence matrix network, and the pH of the essence is 5.5 to 6.

5.

8. A method for preparing an anti-wrinkle essence according to any one of claims 1 to 7, characterized in that, Includes the following steps: S1. Mix a portion of water with a polyol moisturizer and a membrane flexibility regulating component to obtain the liposome hydrated phase; mix the remaining water with xanthan gum, carbomer, emulsifying and matrix stabilizing components and a pH adjuster to obtain the essence matrix aqueous phase; S2. Under light-proof, inert atmosphere and low temperature conditions, phospholipids, tocopheryl acetate and Panax notoginseng oil are mixed to obtain a lipid-phase premix containing Panax notoginseng oil. S3. The lipid premix containing Panax notoginseng oil is slowly added dropwise to the hydration phase of the liposomes to form a preliminary dispersion under stirring conditions. The preliminary dispersion is then subjected to high-pressure homogenization to obtain a flexible nanoliposome dispersion. S4. The flexible nanoliposome dispersion is incorporated into the aqueous phase of the essence matrix, vacuum degassing is performed, a preservative system is added, the pH is adjusted, and the mixture is stirred evenly to obtain the essence with anti-wrinkle effects.

9. The method according to claim 8, characterized in that, In step S2, the preparation temperature of the lipid phase premix is ​​30-45℃. In step S3, the lipid phase premix containing Panax notoginseng oil is added to the liposome hydration phase by slow dropwise addition. The high-pressure homogenization pressure is 600-1000 bar, the number of cycles is 3-5, and the material temperature during the homogenization process does not exceed 35℃.

10. The method according to claim 8, characterized in that, Nitrogen protection is used in step S2. In step S4, the vacuum degassing vacuum degree is -0.06 to -0.09 MPa and the degassing time is 5 to 20 min. The flexible nanoliposome dispersion is incorporated into the aqueous phase of the essence matrix at a temperature not higher than 30°C. The preservative system is added at a temperature not higher than 30°C. The pH of the resulting essence is adjusted to 5.5 to 6.5, and the stirring speed is 300 to 800 rpm.

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