An immune-enhancing nutritional paste for pets, and a method of preparing and using the same

Abstract

The application discloses a kind of pet immunopotentiating nutrition cream and its preparation method and application, and is related to pet health care technical field, component includes astragalus polysaccharide 5 parts, salvia extract 0.5 parts, palm ginseng powder 0.05 parts, chicken oil 3 parts, chicken 2 parts, flaxseed oil 15 parts, lysophospholipid 12 parts, sucrose fatty acid ester 7 parts, sodium chloride 3 parts, yeast iron 0.6 parts, sodium carboxymethyl cellulose 8 parts, potassium sorbate 0.18-0.20 parts, ethylenediaminetetraacetic acid disodium 0.023-0.025 parts, compound B group vitamin 0.0011-0.0013 parts, fructose syrup 0.5 parts, butylated hydroxyanisole (BHA) 0.02 parts, malt dextrin 50 parts, the nutrition cream of the application has the characteristics of immunopotentiating effect, high absorption and utilization rate, good palatability, stability and high safety, significantly improve pet immunoglobulin level (IgG, IgM, IgA is increased by 278%, 417%, 555% respectively), enhance vaccine antibody response (cat plague antibody is increased by 71%), improve cellular immune function (T cell proliferation ability is increased by 66%, CD4+ / CD8+ Ratio is significantly increased).

Description

Technical Field

[0001] This invention relates to the field of pet health products technology, specifically to a pet immune-enhancing nutritional paste, its preparation method, and its application. Background Technology

[0002] With the increasing number of pet owners, pet health issues are receiving growing attention. Weakened immunity is a significant cause of various diseases in pets, especially puppies, senior pets, and those under stress, whose immune functions decline, making them more susceptible to viruses, bacteria, and other pathogens. Currently, common pet immune-boosting products on the market mainly include: chemically synthesized immune enhancers (such as levamisole and thymosin); single herbal extracts (such as astragalus polysaccharides and ganoderma polysaccharides); general nutritional supplements (such as vitamins and trace elements); and traditional nutritional pastes, which are mostly high in energy and fat, primarily functioning to replenish energy and improve coat condition. While chemically synthesized immune enhancers have some immunomodulatory effects, long-term use may produce side effects and does not align with the trend towards natural and safe pet health products. Single herbal extracts, although offering some immune-boosting effects, have limited efficacy due to their single component and cannot comprehensively regulate the body's immune function. General nutritional supplements only provide basic nutrition and lack targeted immunomodulatory effects. Traditional nutritional pastes lack systematic immune-boosting formula design, and their palatability and stability need improvement.

[0003] Therefore, current products generally suffer from the following shortcomings: unclear immune-enhancing effects: most products lack systematic verification of immune indicators, making it difficult to quantify efficacy; single ingredients and weak synergistic effects: single ingredients are insufficient to fully activate the body's immune system; low absorption and utilization rates: some active ingredients are poorly absorbed in the body, resulting in low bioavailability; poor palatability: pets commonly refuse to eat, affecting long-term effectiveness; and poor stability: the paste is prone to separation and deterioration, resulting in a short shelf life. Therefore, developing a pet immune-enhancing nutritional paste with natural ingredients, significant immune-enhancing effects, good palatability, and high stability has significant market value and application prospects. Summary of the Invention

[0004] The purpose of this invention is to provide an immune-enhancing nutritional paste for pets, its preparation method, and its application, in order to solve the existing problems mentioned in the background art.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a pet immune-enhancing nutritional paste, its preparation method, and its application, comprising 5 parts of Astragalus polysaccharide, 0.5 parts of Salvia miltiorrhiza extract, 0.05 parts of Panax ginseng powder, 3 parts of chicken oil, 2 parts of chicken meat, 15 parts of flaxseed oil, 12 parts of lysophosphatidylcholine, 7 parts of sucrose fatty acid ester, 3 parts of sodium chloride, 0.6 parts of yeast iron, 8 parts of sodium carboxymethyl cellulose, 0.18-0.20 parts of potassium sorbate, 0.023-0.025 parts of disodium EDTA, 0.0011-0.0013 parts of compound B vitamins, 0.5 parts of fructose syrup, 0.02 parts of butylated hydroxyanisole (BHA), and 50 parts of maltodextrin; The preparation method of the pet immune-enhancing nutritional paste is as follows: S1. Mix 5 parts of Astragalus polysaccharide, 0.5 parts of Salvia miltiorrhiza extract, 0.05 parts of Panax ginseng powder, 3 parts of chicken oil, 15 parts of flaxseed oil, and 7 parts of sucrose fatty acid ester with an appropriate amount of purified water, and put them into a homogenizing emulsifier to form a uniform and stable emulsion; this allows the oil-soluble and water-soluble components to be fully mixed to form a stable emulsion, improving the uniformity of subsequent mixing and the stability of the product. Among them, Astragalus polysaccharide can be replaced by Ganoderma lucidum polysaccharide, Lentinus edodes polysaccharide, etc., which have similar immune-enhancing effects; Salvia miltiorrhiza extract can be replaced by Panax notoginseng extract, Carthamus tinctorius extract, and other blood-activating and stasis-removing herbs; Flaxseed oil can be replaced by fish oil, Perilla seed oil, and other oils rich in ω-3 fatty acids; Lysolecithin can be replaced by soybean lecithin, egg yolk lecithin, etc.

[0006] Furthermore, in step S2, mince two portions of chicken and add them to the emulsion obtained in step one, stirring well to form a mixture; this improves the palatability of the product and increases the pet's willingness to eat it. Furthermore, in step S3, the remaining ingredients (excluding B vitamins) (including 3 parts sodium chloride, 0.6 parts yeast iron, 8 parts sodium carboxymethyl cellulose, 0.18-0.20 parts potassium sorbate, 0.023-0.025 parts disodium EDTA, 0.5 parts fructose syrup, 0.02 parts butylated hydroxyanisole, and 50 parts maltodextrin) are added to the above mixture, stirred evenly, and placed in a water bath for continuous stirring to perform a maturation process. High-temperature maturation can kill potential microorganisms, gelatinize starch components, improve the texture of the paste, and promote the full integration of various components. Furthermore, after S4 and the curing process is complete, remove the paste and let it stand at 25°C for 30 minutes to allow it to cool naturally to room temperature; this avoids damaging vitamins when added at high temperatures and also helps stabilize the paste structure. Furthermore, in step S5, after the paste cools down to 25°C, add 0.0011 to 0.0013 parts of compound B vitamins, place it in a water bath and stir continuously until a uniform paste is formed, thus obtaining the pet immune-enhancing nutritional paste; adding vitamins at a lower temperature prevents heat-sensitive components from being destroyed and ensures product efficacy.

[0007] Specifically, the liquid mixed in step S1 needs to be homogenized and emulsified in a homogenizer at a speed of 3000-5000 r / min for 10 minutes to form a uniform and stable emulsion.

[0008] Specifically, the mixture obtained in step S3 needs to be continuously stirred in a 96°C water bath for 13 minutes.

[0009] Specifically, in step S5, the paste after adding 0.0011 to 0.0013 parts of compound B vitamins needs to be continuously stirred and mixed in a 35°C water bath for 5 minutes.

[0010] Furthermore, for routine health care, feed 2-3 times a day, 10-25g each time (adjust according to the pet's weight), which can be fed directly or mixed into the main food.

[0011] Furthermore, during the immune enhancement period (before and after vaccination, recovery from illness, stress, etc.), feed 15-30g three times a day for 2-4 weeks.

[0012] Compared with the prior art, the beneficial effects of the present invention are: 1. By using Astragalus polysaccharide as the main immunomodulatory component, supplemented by Salvia miltiorrhiza extract to improve microcirculation and Gynostemma pentaphyllum to nourish and strengthen the body, combined with nutritional support from flaxseed oil, yeast iron, and complex B vitamins, the multi-component synergistic effect utilizes the functions of Astragalus polysaccharide to enhance macrophage activity and promote antibody production, Salvia miltiorrhiza extract to improve microcirculation and enhance immune cell activity, and Gynostemma pentaphyllum powder to improve the body's adaptability, to achieve multi-target immune regulation, comprehensively regulate the body's immune function, significantly increase the level of pet immunoglobulins (IgG, IgM, and IgA increased by 278%, 417%, and 555%, respectively), enhance vaccine antibody response (feline panleukopenia antibody increased by 71%), and improve cellular immune function (T cell proliferation increased by 66%, CD4+ / CD8+ ratio significantly increased), demonstrating a clear immune enhancement effect.

[0013] 2. By using lysophosphatidylcholine and sucrose fatty acid esters as emulsifiers, fat-soluble components (chicken oil, flaxseed oil) and water-soluble components (astragalus polysaccharide, salvia miltiorrhiza extract) are fully emulsified, improving the bioavailability of active ingredients. In addition, palatable ingredients such as chicken meat, chicken oil, and fructose syrup are added, making pets more willing to eat and easy to feed long-term.

[0014] 3. Through processes such as emulsification, maturation, and low-temperature vitamin addition, the paste is uniform and stable, and is not prone to separation or deterioration. Antioxidants and preservatives are added to extend the shelf life. The process involves first preparing an emulsion, then adding minced meat, maturing at high temperature, allowing it to cool down, and finally adding heat-sensitive vitamins at low temperature. This ensures product stability and safety while preserving the active ingredients to the greatest extent.

[0015] 4. By using all food-grade ingredients, there are no side effects from chemically synthesized immune enhancers, making it suitable for long-term use by pets. In addition to immune enhancement, it simultaneously supplements high-quality protein (chicken), unsaturated fatty acids (flaxseed oil), organic iron (yeast iron), B vitamins, etc., achieving synergistic nutritional support and immune regulation. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the bar chart comparing changes in body weight and immunoglobulin levels according to the present invention. Figure 2 This is a schematic diagram of the columnar structure for comparing vaccine antibody levels according to the present invention; Figure 3 This is a schematic diagram of the columnar structure comparing the proliferation and subset changes of T lymphocytes according to the present invention. Detailed Implementation

[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0018] Please see Figure 1-3 This invention provides a pet immune-enhancing nutritional paste, its preparation method, and application technology: the components include 5 parts of Astragalus polysaccharide, 0.5 parts of Salvia miltiorrhiza extract, 0.05 parts of Panax ginseng powder, 3 parts of chicken oil, 2 parts of chicken meat, 15 parts of flaxseed oil, 12 parts of lysophosphatidylcholine, 7 parts of sucrose fatty acid ester, 3 parts of sodium chloride, 0.6 parts of yeast iron, 8 parts of sodium carboxymethyl cellulose, 0.18-0.20 parts of potassium sorbate, 0.023-0.025 parts of disodium EDTA, 0.0011-0.0013 parts of compound B vitamins, 0.5 parts of fructose syrup, 0.02 parts of butylated hydroxyanisole (BHA), and 50 parts of maltodextrin; The preparation method of pet immune-boosting nutritional paste is as follows: S1. Mix 5 parts of Astragalus polysaccharide, 0.5 parts of Salvia miltiorrhiza extract, 0.05 parts of Panax ginseng powder, 3 parts of chicken oil, 15 parts of flaxseed oil, and 7 parts of sucrose fatty acid ester with an appropriate amount of purified water, and put them into a homogenizing emulsifier to form a uniform and stable emulsion; this allows the oil-soluble and water-soluble components to be fully mixed to form a stable emulsion, improving the uniformity of subsequent mixing and the stability of the product. Among them, Astragalus polysaccharide can be replaced by Ganoderma lucidum polysaccharide, Lentinus edodes polysaccharide, etc., which have similar immune-enhancing effects; Salvia miltiorrhiza extract can be replaced by Panax notoginseng extract, Carthamus tinctorius extract, and other blood-activating and stasis-removing herbs; Flaxseed oil can be replaced by fish oil, Perilla seed oil, and other oils rich in ω-3 fatty acids; Lysolecithin can be replaced by soybean lecithin, egg yolk lecithin, etc.

[0019] Furthermore, in step S2, mince two portions of chicken and add them to the emulsion obtained in step one, stirring well to form a mixture; this improves the palatability of the product and increases the pet's willingness to eat it. Furthermore, in step S3, the remaining ingredients (excluding B vitamins) (including 3 parts sodium chloride, 0.6 parts yeast iron, 8 parts sodium carboxymethyl cellulose, 0.18-0.20 parts potassium sorbate, 0.023-0.025 parts disodium EDTA, 0.5 parts fructose syrup, 0.02 parts butylated hydroxyanisole, and 50 parts maltodextrin) are added to the above mixture, stirred evenly, and placed in a water bath for continuous stirring to perform a maturation process. High-temperature maturation can kill potential microorganisms, gelatinize starch components, improve the texture of the paste, and promote the full integration of various components. Furthermore, after S4 and the curing process is complete, remove the paste and let it stand at 25°C for 30 minutes to allow it to cool naturally to room temperature; this avoids damaging vitamins when added at high temperatures and also helps stabilize the paste structure. Furthermore, S5, after the paste cools down to 25°C, add 0.0011 to 0.0013 parts of compound B vitamins, place it in a water bath and stir continuously until a uniform paste is formed, which is the pet immune-enhancing nutritional paste; adding vitamins at a lower temperature prevents heat-sensitive components from being destroyed and ensures product efficacy.

[0020] Specifically, the liquid mixed in step S1 needs to be homogenized and emulsified in a homogenizer at a speed of 3000-5000 r / min for 10 minutes to form a uniform and stable emulsion.

[0021] Specifically, the mixture obtained in step S3 needs to be continuously stirred in a 96°C water bath for 13 minutes.

[0022] Specifically, after adding 0.0011 to 0.0013 parts of compound B vitamins in step S5, the paste needs to be continuously stirred and mixed in a 35°C water bath for 5 minutes.

[0023] Specifically, for daily health care, feed 2-3 times a day, 10-25g each time (adjust according to the pet's weight), which can be fed directly or mixed into the main food.

[0024] Specifically, during the immune enhancement period (before and after vaccination, recovery from illness, stress, etc.), feed 15-30g three times a day for 2-4 weeks.

[0025] Example 1 (Preferred Formula and Process) Prepare the following formula by weight: 5 parts Astragalus polysaccharide, 0.5 parts Salvia miltiorrhiza extract, 0.05 parts Panax ginseng powder, 3 parts chicken oil, 2 parts chicken meat, 15 parts flaxseed oil, 12 parts lysophosphatidylcholine, 7 parts sucrose fatty acid ester, 3 parts sodium chloride, 0.6 parts yeast iron, 8 parts sodium carboxymethyl cellulose, 0.19 parts potassium sorbate, 0.024 parts disodium EDTA, 0.0012 parts complex B vitamins, 0.5 parts fructose syrup, 0.02 parts BHA, and 50 parts maltodextrin. Follow the above steps to obtain the nutritional paste.

[0026] Example 2 (Endpoint Recipe 1) Take 0.18 parts of potassium sorbate, 0.023 parts of disodium ethylenediaminetetraacetate, and 0.0011 parts of compound B vitamins. The rest is the same as in Example 1.

[0027] Example 3 (Endpoint Recipe 2) Take 0.20 parts of potassium sorbate, 0.025 parts of disodium ethylenediaminetetraacetate, and 0.0013 parts of B vitamins. The rest is the same as in Example 1.

[0028] Effect test To verify the immune-enhancing effect of the nutritional paste of the present invention, the following animal experiments were conducted.

[0029] Experiment 1: Changes in body weight and immunoglobulin levels Ten healthy, disease-free lion cats of similar weight and age were randomly divided into two groups of five each: a nutritional paste group and a control group. The nutritional paste group was fed the nutritional paste prepared in Example 1 of this invention daily, while the control group was fed regular cat food. Feeding was conducted daily at 8:00, 12:00, and 18:00, with each feeding being 25g. After 10 weeks of feeding, the cats' weight was measured, and serum immunoglobulin IgG, IgA, and IgM levels were measured. The changes and average values ​​were calculated, and the results are shown in Table 1.

[0030] Table 1. Changes in body weight and immunoglobulin levels Results analysis: After feeding the nutritional paste of this invention for 10 weeks, the weight gain of the pet cats was significantly higher than that of the control group (approximately 281%), and the levels of immunoglobulins IgG, IgM, and IgA were approximately 278%, 417%, and 555% higher than those of the control group, respectively. This indicates that the nutritional paste of this invention can significantly promote the growth of pets and improve humoral immune function.

[0031] Experiment 2: Changes in vaccine antibody levels and cellular immune function Ten healthy, disease-free cats (Lion cats) of similar weight and age were randomly divided into two groups of five each. Before the experiment, all cats were vaccinated with "Fish Triple Vaccine" (feline panleukopenia, herpes simplex virus, and calicivirus trivalent vaccine), designated as the nutritional paste group and the blank control group. The nutritional paste group was fed the nutritional paste prepared in Example 1 of this invention daily, while the blank control group was fed regular cat food. Feeding was conducted daily at 8:00, 12:00, and 18:00, with each feeding being 25g. After 10 weeks of feeding, the antibody levels for feline panleukopenia virus, herpes simplex virus, and calicivirus were detected using an ELISA kit; peripheral blood T lymphocyte proliferation (A570 value), CD4+ T cells, and CD8+ T cell percentages were measured, and the CD4+ / CD8+ ratio was calculated. The results are shown in Table 2.

[0032] Table 2 Changes in vaccine antibody levels and cellular immune function Results analysis: After feeding the nutritional paste of this invention for 10 weeks, the antibody levels for feline panleukopenia virus, herpesvirus, and calicivirus in the nutritional paste group increased by approximately 71%, 56%, and 34% respectively compared to the control group, indicating that the nutritional paste of this invention can significantly enhance the immune response to vaccines and increase the level of specific antibodies. The peripheral blood T lymphocyte proliferation capacity (A570 value) increased by approximately 66% compared to the control group, and the proportions of CD4+ T cells, CD8+ T cells, and the CD4+ / CD8+ ratio were all significantly higher than those in the control group, indicating that the nutritional paste of this invention can effectively enhance cellular immune function.

[0033] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, the phrase "comprising an element defined as..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes said element.

[0034] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A pet immune-boosting nutritional paste, characterized in that, The ingredients include 5 parts Astragalus polysaccharide, 0.5 parts Salvia miltiorrhiza extract, 0.05 parts Panax ginseng powder, 3 parts chicken oil, 2 parts chicken meat, 15 parts flaxseed oil, 12 parts lysophosphatidylcholine, 7 parts sucrose fatty acid ester, 3 parts sodium chloride, 0.6 parts yeast iron, 8 parts sodium carboxymethyl cellulose, 0.18-0.20 parts potassium sorbate, 0.023-0.025 parts disodium EDTA, 0.0011-0.0013 parts complex B vitamins, 0.5 parts fructose syrup, 0.02 parts butylated hydroxyanisole (BHA), and 50 parts maltodextrin. The preparation method of the pet immune-enhancing nutritional paste is as follows: S1. Mix 5 parts of Astragalus polysaccharide, 0.5 parts of Salvia miltiorrhiza extract, 0.05 parts of Codonopsis pilosula powder, 3 parts of chicken oil, 15 parts of flaxseed oil, and 7 parts of sucrose fatty acid ester with an appropriate amount of purified water, and put them into a homogenizing emulsifier to form a uniform and stable emulsion. S2. Mince two portions of chicken and add them to the emulsion obtained in step one. Stir well to form a mixture. S3. Add the remaining ingredients (excluding B vitamins) (including 3 parts sodium chloride, 0.6 parts yeast iron, 8 parts sodium carboxymethyl cellulose, 0.18-0.20 parts potassium sorbate, 0.023-0.025 parts disodium EDTA, 0.5 parts fructose syrup, 0.02 parts butylated hydroxyanisole, and 50 parts maltodextrin) to the above mixture, stir evenly, place in a water bath and stir continuously for maturation. S4. After curing, remove the paste and let it stand in a 25℃ environment for 30 minutes to allow it to cool down to room temperature naturally. S5. After the paste cools down to 25°C, add 0.0011 to 0.0013 parts of compound B vitamins, place it in a water bath and stir continuously until a uniform paste is formed, which is the pet immune-enhancing nutritional paste.

2. The pet immune-enhancing nutritional paste according to claim 1, characterized in that: The liquid mixed in step S1 needs to be homogenized and emulsified in a homogenizer at a speed of 3000-5000 r / min for 10 minutes to form a uniform and stable emulsion.

3. The pet immune-enhancing nutritional paste according to claim 1, characterized in that: The mixture obtained in step S3 needs to be continuously stirred in a 96°C water bath for 13 minutes.

4. The pet immune-enhancing nutritional paste according to claim 1, characterized in that: In step S5, the paste after adding 0.0011 to 0.0013 parts of compound B vitamins needs to be continuously stirred and mixed in a 35°C water bath for 5 minutes.

5. The application of the pet immune-enhancing nutritional paste according to claim 1, characterized in that: For routine health care, feed 2-3 times a day, 10-25g each time (adjust according to the pet's weight), which can be fed directly or mixed into the main food.

6. The application of the pet immune-enhancing nutritional paste according to claim 1, characterized in that: During the immune enhancement period (before and after vaccination, recovery from illness, stress, etc.), feed 15-30g three times a day for 2-4 weeks.

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