Sterile culture system

Abstract

The utility model relates to the technical field of biopharmaceutical equipment, in particular to a sterile culture system. The sterile culture system comprises a cell preparation device, an incubator and a cell culture device, the cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; the incubator is provided with a culture chamber, a second sealing valve is arranged on the side wall of the culture chamber, and the second sealing valve is used for being matched with the first sealing valve, so that the incubator and the cell preparation device are in sealed butt joint or sealed separation; the cell culture device is used for accommodating the culture box, supplying power and gas to the culture box and controlling the cell growth environment in the culture box. As the cells always grow in the sterile environment, the pollution risk is low, frequent sterilization is not needed, the normal growth of the cells is prevented from being influenced by frequent sterilization, the culture time is saved, and the cell preparation and culture efficiency is high.

Description

Technical Field

[0001] The utility model relates to the technical field of biopharmaceutical equipment, in particular to an aseptic culture system. Background Art

[0002] CGT (cell and gene therapy) products are cutting-edge technologies in the medical field, with high added value and huge market potential. They are part of the drugs that cure diseases and save lives. Like conventional chemical drugs, they must also ensure the sterility of the production process. Once contaminated during the production process, patients will lose the best time for treatment due to inability to obtain drugs in time. In severe cases, the patient may lose his life, and CGT pharmaceutical companies will suffer heavy losses.

[0003] In addition, CGT drug culture requires strict time control. If the preparation and culture process is not well controlled, it will affect the cell growth, and the cell culture situation will directly affect the treatment effect of patients receiving cell therapy. CGT drugs are biologically active drugs, and they need to be observed and replaced with culture media at any time during the culture process. The traditional way is to transfer the cell drug to a conventional incubator for culture after it is prepared in the cell preparation device. When observation and culture media replacement are required during the culture process, it is transferred to the cell preparation device for processing. After completion, it is transferred to the incubator for continued culture, and the operation is repeated repeatedly.

[0004] During the reciprocating movement of cell medicines from the cell preparation device to the incubator, the cell culture container will pass through the B / C / D low-level environment of the laboratory, and the probability of cell contamination is extremely high; the personnel performing the transfer operation need to have very professional skills, and the operator's condition will directly affect the risk of cell contamination during the transfer process; the entire culture process of conventional incubators is in a low-level environment, and the cell culture process has a high risk of contamination. In addition, the culture container containing cells is frequently sterilized, and the sterilant has the risk of penetrating the cell container and killing the cells. Therefore, the cell culture process of the existing aseptic culture method has the problems of high contamination risk and low cell preparation and culture efficiency. Utility Model Content

[0005] The utility model aims to provide a sterile culture system to solve the problems of high contamination risk and low cell preparation and culture efficiency in the process of culturing cells in the prior art.

[0006] In order to solve the above technical problems, the utility model provides a sterile culture system.

[0007] The aseptic culture system of the utility model comprises a cell preparation device, a culture box and a cell culture device;

[0008] The cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber;

[0009] The incubator has a culture chamber, and a second sealing valve is provided on the side wall of the culture chamber, and the second sealing valve is used to cooperate with the first sealing valve to achieve sealed docking or sealed separation between the incubator and the cell preparation device;

[0010] The cell culture device is used to accommodate a culture box, supply power and gas to the culture box, and control the cell growth environment in the culture box.

[0011] Further, one of the first sealing valve and the second sealing valve is an RTP main valve, and the other is an RTP auxiliary valve, and the RTP main valve and the RTP auxiliary valve form an RTP valve when they are buckled together.

[0012] Further, the edge of the RTP main valve is provided with a first cylinder and a second cylinder for rotating the RTP main valve, and the extension and retraction directions of the first cylinder and the second cylinder are set to be parallel and opposite;

[0013] The RTP main valve or the RTP auxiliary valve is provided with a switch gate mechanism, and the switch gate mechanism is used to open or close the RTP valve.

[0014] Furthermore, the cell preparation device also includes a sterilization device for generating sterilization gas, and an output port of the sterilization device is connected to the preparation chamber.

[0015] Furthermore, the cell preparation device also includes a circulating fan, and the incubator is provided with a turbulent fan. The circulating fan and the turbulent fan cooperate to fully diffuse the sterilizing gas in the preparation chamber and the culture chamber.

[0016] Furthermore, the cell preparation device also includes an exhaust component, which is used to replace the sterilization gas and discharge it after the sterilization is completed.

[0017] Further, it also includes: a vacuum pump and a suction pipeline;

[0018] A sterilizing filter is provided in the incubator, and the sterilizing filter is connected to the culture chamber through a gas pipeline;

[0019] The suction pipeline is connected to the sterilizing filter, and the vacuum pump is arranged on the suction pipeline to suck the sterilizing gas in the incubator to sterilize the sterilizing filter.

[0020] Furthermore, it also includes: an air blowing pipeline, which is connected to the sterilizing filter and is used to blow air into the sterilizing filter to discharge the sterilizing gas in the sterilizing filter after the sterilization is completed.

[0021] Furthermore, the cell culture device has a plurality of containing spaces arranged in an array, and the culture boxes placed in the containing spaces are centrally controlled by the control unit of the cell culture device.

[0022] Furthermore, it also includes a transfer trolley, which is used to transfer the incubator.

[0023] Compared with the prior art, the utility model has at least the following beneficial effects:

[0024] Before use, the incubator and the cell preparation device are sealed and docked, so that the gas environment of the culture chamber and the gas environment of the preparation chamber are connected, and then the two are sterilized, and after the sterilization is completed, the two are sealed and separated. Then, cells are prepared in the preparation chamber, and after the cell preparation is completed, the second sealing valve of the incubator is matched with the first sealing valve of the cell preparation device, so that the incubator and the cell preparation device are sealed and docked, so that the gas environment of the culture chamber and the gas environment of the preparation chamber are connected, and in this sealed sterile environment, the cells are transferred to the incubator, and then the incubator and the cell preparation device are sealed and separated, and the incubator is transferred to the cell culture device to culture the cells.

[0025] Because the cell preparation device and incubator have been sterilized before preparing the cells, the prepared cells grow in a sterile environment. Also, because the incubator and the cell preparation device are sealed and docked before the cells are transferred to the incubator, and the cells are transferred to the incubator in a sealed environment, the cells always grow in a sterile environment with a low risk of contamination, so frequent sterilization is not required, which not only avoids affecting the normal growth of cells due to frequent sterilization, but also saves culture time, so the efficiency of cell preparation and culture is high. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a structural schematic diagram of an embodiment of the sterile culture system of the utility model;

[0027] Figure 2 for Figure 1 A schematic diagram of the structure of the cell preparation device and the incubator when they are connected and sterilized together;

[0028] Figure 3 for Figure 1 A schematic diagram of the structure after some structures are hidden when the cell preparation device and the incubator are docked;

[0029] Figure 4 for Figure 1 A schematic diagram of the structure of the cell preparation device in which part of the structure is hidden to show the RTP main valve;

[0030] Figure 5 for Figure 4 A local enlarged view of point A in FIG.

[0031] Reference numerals:

[0032] 100. Cell preparation device; 110. Culture chamber; 120. Support frame; 130. Limiting member; 141. Main valve body; 142. Main valve plate; 143. First rotary drive cylinder; 144. Second rotary drive cylinder; 145. Driving motor; 146. Connecting rod assembly; 147. Latch shaft; 148. Hinge movable leaf; 149. Support block; 150. Latch seat; 151. RTP auxiliary valve; 161. Control unit; 162. First in-position signal sensor; 163. Second in-position signal sensor; 164. Third in-position signal sensor; 165. Fourth in-position signal sensor; 166. Power supply socket; 171. Liquid storage bottle; 172. Delivery pump; 173. Delivery pipeline; 174. Gas generating device; 175. Circulating fan; 176. Exhaust assembly;

[0033] 200, incubator; 210, turbulent fan; 221, first sterilizing filter; 222, second sterilizing filter; 223, suction pipeline; 225, first two-position two-way solenoid valve; 226, vacuum pump; 227, air blowing pipeline; 228, second two-position two-way solenoid valve; 229, compressed air flow regulating valve;

[0034] 300. Cell culture device;

[0035] 400. Transfer the cart. DETAILED DESCRIPTION

[0036] A sterile culture system of the utility model will be described below in conjunction with a schematic diagram, wherein a preferred embodiment of the utility model is shown, and it should be understood that those skilled in the art can modify the utility model described herein, and still achieve the beneficial effects of the utility model. Therefore, the following description should be understood as being widely known to those skilled in the art, and not as a limitation to the utility model.

[0037] The serial numbers assigned to the components herein, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings). In the description of the present utility model, it should be understood that the orientation or position relationship indicated by the terms "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. are based on the orientation or position relationship shown in the accompanying drawings, which is only for the convenience of describing the present utility model and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation to the present utility model.

[0038] In the present utility model, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may mean that the first and second features are in direct contact, or the first and second features are in indirect contact through an intermediate medium. Moreover, a first feature being "above", "above" or "above" a second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is higher in level than the second feature. A first feature being "below", "below" or "below" a second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature is lower in level than the second feature.

[0039] The present invention is described in more detail in the following paragraphs by way of example with reference to the accompanying drawings. The advantages and features of the present invention will become more apparent from the following description and claims. It should be noted that the accompanying drawings are in very simplified form and in non-precise proportions, and are only used to conveniently and clearly assist in explaining the purpose of the embodiments of the present invention.

[0040] The following is attached to the instruction manual Figures 1 to 5 , the sterile culture system of the utility model is introduced.

[0041] like Figure 1 and Figure 2 As shown, in one embodiment, the sterile culture system of this embodiment includes a cell preparation device 100, an incubator 200 and a cell culture device 300.

[0042] The cell preparation device 100 includes a preparation chamber, and a first sealing valve is disposed on the side wall of the preparation chamber.

[0043] The culture box 200 has a culture chamber 110 , and a second sealing valve is provided on the side wall of the culture chamber 110 . The second sealing valve is used to cooperate with the first sealing valve to achieve sealed docking or sealed separation of the culture box 200 and the cell preparation device 100 .

[0044] The cell culture device 300 is used to accommodate the incubator 200, supply power and gas to the incubator, and control the cell growth environment in the incubator.

[0045] Before use, the incubator 200 and the cell preparation device 100 are sealed and docked, so that the gas environment of the culture chamber 110 is connected to the gas environment of the preparation chamber, and then the two are sterilized. After the sterilization is completed, the two are sealed and separated. Then, cells are prepared in the preparation chamber. After the cell preparation is completed, the second sealing valve of the incubator 200 is matched with the first sealing valve of the cell preparation device 100, so that the incubator 200 and the cell preparation device 100 are sealed and docked, so that the gas environment of the culture chamber 110 is connected to the gas environment of the preparation chamber. In this sealed sterile environment, the cells are transferred to the incubator 200, and then the incubator 200 and the cell preparation device 100 are sealed and separated, and the incubator 200 is transferred to the cell culture device 300 to culture the cells.

[0046] Because the cell preparation device 100 and the incubator 200 have been sterilized before preparing the cells, the prepared cells grow in a sterile environment. Also, because the incubator 200 and the cell preparation device 100 are sealed and docked before the cells are transferred to the incubator 200, and the cells are transferred to the incubator 200 in a sealed environment, the cells always grow in a sterile environment, and the risk of contamination is low, so frequent sterilization is not required, which not only avoids affecting the normal growth of cells due to frequent sterilization, but also saves culture time, so the efficiency of cell preparation and culture is high.

[0047] Specifically, the cell preparation device 100 also includes a support frame 120, which is used to support the incubator 200. A limit member 130 is provided on the support frame 120, and the limit member 130 is used to limit the position of the incubator 200 so as to firmly fix the incubator 200 on the support frame 120.

[0048] In order to achieve sterile transfer, a sterile operation window is provided on the side wall of the preparation chamber, and sterile operation gloves, such as rubber gloves, are provided at the sterile operation window. The sterile operation gloves are located in the preparation chamber and are sealed to the sterile operation window. The operator only needs to insert both hands into the sterile operation gloves to perform operations without opening the preparation chamber, such as transferring cells in the preparation chamber to the docked incubator 200, or transferring cells in the docked incubator 200 to the preparation chamber.

[0049] In one embodiment, if Figure 3 , Figure 4 and Figure 5As shown, one of the first sealing valve and the second sealing valve is an RTP (Rapid Transfer Ports) main valve, and the other is an RTP secondary valve 151. Preferably, the first sealing valve is an RTP main valve, and the second sealing valve is an RTP secondary valve 151, that is, an RTP main valve is provided in the cell preparation device 100, and an RTP secondary valve 151 is provided in the incubator 200.

[0050] After the RTP main valve and the RTP auxiliary valve 151 are buckled together, the outer surface of the RTP main valve and the outer surface of the RTP auxiliary valve 151 are sealed in the RTP valve. The outer surface refers to the side exposed to the external environment, and if there are bacteria, they will be sealed between the RTP main valve and the RTP auxiliary valve 151. By opening the RTP valve formed by the RTP main valve and the RTP auxiliary valve 151, the two sterile chambers can be connected.

[0051] The process of achieving sealed docking between the incubator and the cell preparation device using the sealed docking structure includes: rotating the RTP main valve so that the RTP main valve and the RTP auxiliary valve 151 are engaged to form an RTP valve; opening the RTP valve to achieve sealed docking between the incubator and the cell preparation device.

[0052] The process of using the sealing docking structure to achieve sealed separation of the incubator and the cell preparation device includes: closing the RTP valve; rotating the RTP main valve to separate the RTP main valve and the RTP auxiliary valve 151 to achieve sealed separation of the incubator and the cell preparation device.

[0053] By using the RTP main valve and the RTP auxiliary valve 151, the sealed docking or sealed separation of the cell preparation device 100 and the incubator 200 can be automatically achieved, and the transfer of cells is relatively convenient, without the need for professional skills. General laboratory assistants can complete the entire transfer process through simple operations, and the labor cost investment is relatively low. Of course, in other embodiments, the RTP auxiliary valve 151 can also be used as the first sealing valve, and the RTP main valve can be used as the second sealing valve.

[0054] Specifically, the RTP main valve includes a main valve body 141 and a main valve plate 142 . A rotation driving mechanism is disposed on the edge of the RTP main valve, and a switch gate mechanism is disposed on the RTP main valve.

[0055] The main valve body 141 is rotatably disposed on one of the side walls of the preparation chamber, and the rotation drive mechanism includes a first rotation drive cylinder 143 and a second rotation drive cylinder 144 whose extension and contraction directions are parallel and opposite to each other. The two rotation drive cylinders are disposed on both sides of the main valve body 141 and can drive the main valve body 141 to rotate. The main valve plate 142 can block the main valve body 141, thereby sealing the preparation chamber to prevent external contamination.

[0056] The door opening and closing mechanism is used to drive the main valve plate 142 to rotate so as to open or close the RTP valve. Specifically, the door opening and closing mechanism includes a driving motor 145, a connecting rod assembly 146, a latch shaft 147 and a hinge movable leaf 148. The driving motor 145 is connected to the hinge movable leaf 148 through a gear, and the driving motor 145 is also connected to the connecting rod assembly 146 through other gears. One side of the hinge movable leaf 148 is fixedly connected to the main valve plate 142. The driving motor 145 can drive the hinge movable leaf 148 to swing, thereby swinging the main valve plate 142 to open or block the main valve body 141.

[0057] A support block 149 is provided on the other side of the hinge movable leaf 148, and a latch seat 150 corresponding to the latch shaft 147 is provided on the main valve body 141. A through hole is provided on the support block 149, and the latch shaft 147 passes through the support block 149. The end thereof can be inserted into the latch seat 150 to lock the main valve plate 142 on the main valve body 141. The other end of the latch shaft 147 is hinged to the connecting rod assembly 146, and the driving motor 145 can control the movement of the latch shaft 147 through the connecting rod assembly 146 for unlocking.

[0058] A main valve disc is arranged on the main valve plate 142, and the RTP auxiliary valve 151 is movably installed on the incubator 200. An auxiliary valve disc is arranged on the RTP auxiliary valve 151. When the RTP main valve and the RTP auxiliary valve 151 are docked, the main valve plate 142 is rotated to a set angle, and the main valve disc and the auxiliary valve disc can be buckled together, thereby fixing the main valve plate 142 and the RTP auxiliary valve 151 together.

[0059] In order to improve the degree of automation, such as Figure 2 , Figure 3 , Figure 4 and Figure 5As shown, the cell preparation device 100 further includes a control unit 161, and a first in-place signal sensor 162, a second in-place signal sensor 163, a third in-place signal sensor 164, and a fourth in-place signal sensor 165 are also provided on the side wall of the preparation chamber. When the third in-place signal sensor 164 receives a signal, the control unit 161 obtains information indicating that the incubator 200 has been docked in place, and at this time, the control unit 161 outputs a control signal to enable the first rotation drive cylinder 143 and the second rotation drive cylinder 144 to drive the main valve body 141 to rotate a set angle.

[0060] When the first in-position signal sensor 162 loses the signal and the second in-position signal sensor 163 obtains the signal, the first rotary drive cylinder 143 and the second rotary drive cylinder 144 stop driving, and the main valve disc of the main valve plate 142 and the sub-valve disc of the RTP sub-valve 151 can be buckled, thereby fixing the main valve plate 142 and the RTP sub-valve 151 together. At this time, the control unit 161 determines that the drive motor 145 is in the action state, forming an interlocking feedback control, and the drive motor 145 controls the movement of the latch shaft 147 through the connecting rod assembly 146, and the end of the latch shaft 147 is disengaged from the latch seat 150 to unlock. At this time, the drive motor 145 continues to rotate, and the main valve plate 142 drives the RTP sub-valve 151 to swing through the swing of the hinge movable leaf 148, so as to open the preparation chamber and the incubator 200 of the preparation module.

[0061] When it is necessary to close the main valve body 141, the control unit 161 outputs a control signal to make the drive motor 145 rotate in the opposite direction, and the main valve plate 142 swings through the hinge movable leaf 148, thereby closing the main valve body 141. As the drive motor 145 continues to rotate, the end of the latch shaft 147 is inserted into the latch seat 150 for locking. When locked in place, the fourth in-place signal sensor 165 receives a signal to determine that the main valve body 141 has been locked.

[0062] Then, the control unit 161 outputs a control signal to make the first rotary drive cylinder 143 and the second rotary drive cylinder 144 drive the main valve body 141 to rotate in the opposite direction until the first in-position signal sensor 162 receives a signal and the second in-position signal sensor 163 loses a signal. At this time, it indicates that the main valve body 141 has rotated into position, the main valve disc and the auxiliary valve disc are no longer engaged, and the main valve plate 142 and the RTP auxiliary valve 151 are no longer fixed together. At this time, the preparation chamber and the incubator 200 of the preparation module are both sealed and can be separated. After separation, the power socket 166 of the cell preparation device 100 no longer supplies power to the incubator 200.

[0063] In one embodiment, if Figure 2As shown, the cell preparation device 100 further includes a sterilization device for generating sterilization gas, and the output port of the sterilization device is connected to the preparation chamber for introducing the sterilization gas into the preparation chamber for sterilization.

[0064] Specifically, in this embodiment, the sterilization device includes a liquid storage bottle 171, a delivery pump 172, a delivery pipeline 173 and a gas generating device 174. The sterilization gas is hydrogen peroxide gas. The liquid storage bottle 171 stores hydrogen peroxide liquid. The delivery pipeline 173 connects the liquid storage bottle 171 with the gas generating device 174. The delivery pump 172 delivers the hydrogen peroxide liquid to the gas generating device 174, and the gas generating device 174 converts the hydrogen peroxide liquid into hydrogen peroxide gas.

[0065] A circulating fan 175 is also provided in the cell preparation device 100, and a turbulent fan 210 is provided in the incubator 200. The circulating fan 175 and the turbulent fan 210 cooperate to fully diffuse the hydrogen peroxide gas in the preparation chamber and the culture chamber 110 to fully sterilize. An exhaust component 176 is also provided in the cell preparation device 100 to replace and discharge the hydrogen peroxide gas after the sterilization is completed. In other embodiments, other sterilizing gases can also be used for sterilization.

[0066] In one embodiment, in order to prevent the external environment from contaminating the incubator 200, a sterilizing filter is provided in the incubator 200, and a suction pipe 223 connected to the sterilizing filter is connected to the outside of the incubator 200. The suction pipe 223 sterilizes the sterilizing filter by sucking the sterilizing gas in the incubator 200.

[0067] The incubator 200 is also connected to a blowing pipeline 227 connected to the sterilizing filter. The blowing pipeline 227 is used to blow air into the sterilizing filter to discharge the sterilizing gas in the sterilizing filter after sterilization. At the same time, the blown gas will enter the culture chamber connected to the sterilizing filter to accelerate the turbulence.

[0068] Specifically, in this embodiment, in order to fully filter and sterilize, two sterilizing filters are provided in the sterilizing filter, namely, a first sterilizing filter 221 and a second sterilizing filter 222. A pipeline quick-plug interface is provided on the incubator 200, and the suction pipeline 223 and the air blowing pipeline 227 are simultaneously connected to the pipeline quick-plug interface. A first two-position two-way solenoid valve 225 and a vacuum pump 226 are provided on the suction pipeline 223, and a second two-position two-way battery valve and a compressed air flow regulating valve 229 are provided on the air blowing pipeline 227. In other embodiments, different numbers of sterilizing filters may also be provided, such as three or four.

[0069] During sterilization, first open the first two-position two-way solenoid valve 225, close the second two-position two-way solenoid valve 228, and use the suction of the vacuum pump 226 to allow the sterilizing gas in the incubator 200 to pass through the first sterilizing filter 221 and the second sterilizing filter 222 and enter the suction pipeline 223, so that the sterilizing gas can fully sterilize the two sterilizing filters.

[0070] After sterilization is completed, the exhaust component 176 is opened to discharge the sterilization gas. At the same time, the first two-position two-way battery valve is closed, the second two-position two-way solenoid valve 228 is opened, and the compressed air flow regulating valve 229 is kept open. Compressed air is introduced into the blowing pipeline 227 to blow the first sterilizing filter 221 and the second sterilizing filter 222, so that the residual sterilizing gas is separated from the two sterilizing filters and is replaced and discharged along with other sterilizing gases.

[0071] In one embodiment, if Figure 1 As shown, the sterile culture system of this embodiment also includes a transfer cart 400, which is used to transfer the incubator 200, for example, to transfer the incubator 200 from the cell culture device 300 to the cell preparation device 100, or to transfer the incubator 200 from the cell preparation device 100 to the incubator 200.

[0072] In one embodiment, the cell culture device 300 in this embodiment has a plurality of accommodating spaces arranged in an array. For example, the cell culture device 300 may be a honeycomb cell culture device 300, which may accommodate a plurality of culture boxes 200 for simultaneous culture. A control unit is provided inside the cell culture device 300 to centrally control the culture process of the plurality of culture boxes 200.

[0073] Before use, the transfer cart 400 is used to dock the incubator 200 with the cell preparation device 100, and then the sterilization device is used to sterilize the preparation chamber and the incubator 200. After the sterilization is completed, the incubator 200 and the cell preparation device 100 are separated. Cells are prepared in the cell preparation device 100. After the cell preparation is completed, the incubator 200 is docked with the cell preparation device 100 again, and the cells are transferred to the incubator 200. Then, the incubator 200 is separated from the cell preparation device 100, and the transfer cart 400 transfers the incubator 200 to the cell culture device 300 for sterile culture. The entire cell transfer process can be completed within 3 minutes, the operation is relatively simple, and the efficiency of cell preparation and culture can be effectively improved.

[0074] When periodic observation and replacement of culture medium are required during the culture process, the incubator 200 is transferred from the cell culture device 300 to the cell preparation device 100 by the transfer cart 400 again, and the previous docking operation process is repeated to complete the docking. After observation and replacement of the culture medium, the incubator 200 is separated from the cell preparation device 100 again, and the transfer cart 400 is used to transfer the incubator 200 from the cell preparation device 100 to the cell culture device 300 to continue the culture, and this process is repeated until the culture is completed.

[0075] The entire culture process has a low risk of contamination and low personnel input costs. The transfer process has a very low impact on cell growth, avoiding the killing of cultured cells by transfer sterilization. The culture process has a low risk of contamination and greatly improves the efficiency of cell preparation and culture.

[0076] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A sterile culture system, characterized in that: It includes a cell preparation device, an incubator and a cell culture device; The cell preparation device comprises a preparation chamber, and a first sealing valve is arranged on the side wall of the preparation chamber; The incubator has a culture chamber, and a second sealing valve is provided on the side wall of the culture chamber, and the second sealing valve is used to cooperate with the first sealing valve to achieve sealed docking or sealed separation between the incubator and the cell preparation device; The cell culture device is used to accommodate a culture box, supply power and gas to the culture box, and control the cell growth environment in the culture box.

2. The sterile culture system according to claim 1, characterized in that: One of the first sealing valve and the second sealing valve is an RTP main valve, and the other is an RTP auxiliary valve. The RTP main valve and the RTP auxiliary valve form an RTP valve when they are buckled together.

3. The sterile culture system according to claim 2, characterized in that: The edge of the RTP main valve is provided with a first cylinder and a second cylinder for rotating the RTP main valve, and the extension and contraction directions of the first cylinder and the second cylinder are set to be parallel and opposite; The RTP main valve or the RTP auxiliary valve is provided with a switch gate mechanism, and the switch gate mechanism is used to open or close the RTP valve.

4. The sterile culture system according to claim 1, characterized in that: The cell preparation device further comprises a sterilization device for generating sterilization gas, and an output port of the sterilization device is communicated with the preparation chamber.

5. The sterile culture system according to claim 4, characterized in that: The cell preparation device further comprises a circulation fan, and a turbulence fan is arranged in the culture box. The circulation fan and the turbulence fan cooperate to fully diffuse the sterilization gas in the preparation chamber and the culture chamber.

6. The sterile culture system according to claim 4, characterized in that: The cell preparation device also includes an exhaust component, which is used to replace and discharge the sterilization gas after the sterilization is completed.

7. The sterile culture system according to claim 4, characterized in that: Also included: a vacuum pump and suction piping; A sterilizing filter is provided in the incubator, and the sterilizing filter is connected to the culture chamber through a gas pipeline; The suction pipeline is connected to the sterilizing filter, and the vacuum pump is arranged on the suction pipeline to suck the sterilizing gas in the incubator to sterilize the sterilizing filter.

8. The sterile culture system according to claim 7, characterized in that: Also includes: The air blowing pipeline is connected to the sterilizing filter and is used to blow air into the sterilizing filter so as to discharge the sterilizing gas in the sterilizing filter after the sterilization is completed.

9. The sterile culture system according to claim 1, characterized in that: The cell culture device has a plurality of accommodating spaces arranged in an array, and the culture boxes placed in the accommodating spaces are centrally controlled by the control unit of the cell culture device.

10. The sterile culture system according to claim 1, characterized in that: Also included is a transfer trolley, which is used to transfer the incubator.

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