Adherent cell culture medium

Abstract

The utility model discloses an adherent cell culture medium, which belongs to the technical field of culture media for adherent cells and comprises a shell, a partition plate arranged in the shell, four cavities arranged between the partition plate and the shell, a culture dish arranged in each cavity, and a solid culture medium arranged at the bottom of the inner side of each culture dish. A pop-up assembly is arranged between the culture dish and the shell, the pop-up assembly is used for popping up the culture dish, the pop-up assembly comprises a threaded rod, the threaded rod is arranged on the lower side of the culture dish, the lower side of the shell is rotationally connected with a nut, the nut is in threaded connection with the threaded rod, and a plurality of springs are arranged between the culture dish and the shell; and an adjustable shading component is arranged on the upper side of the shell and is used for performing different shading adjustments on the culture dishes in the cavity, so that the problem that a plurality of groups of adherent cells are inconvenient to culture and observe by using the same culture medium is solved.

Description

Technical Field

[0001] The utility model relates to the technical field of culture media for adherent cells, in particular to an adherent cell culture medium. Background Art

[0002] Adherent cells, the growth of such cells must have a support surface that can be adhered to, and the cells can grow and reproduce on this surface by relying on the adhesion factors secreted by themselves or provided in the culture medium.

[0003] In the prior art, Chinese Patent No. CN202220188995.9 discloses a cell culture medium. Before use, place the shell horizontally on the desktop, and then the cells can be placed inside the shell. Thus, according to the specific requirements of different cell cultures, the temperature control tube inside can be controlled to work by the controller, and it works under the action of the power supply and the controller to dissipate heat. This heat can be effectively evenly distributed by the constant temperature plate, and then effectively transferred to the shell, so that the temperature inside reaches the set value, achieving automatic temperature control and constant temperature. If the cells need to be shielded from light, the fixed block can be directly pulled, and it will be stressed and deformed under the action of the force, so as to move away from the fixed groove. At this time, the baffle will lose the fixed connection structure, and the light-shielding plate can be effectively placed in the groove inside the cover plate, so as to effectively keep the cells inside the shell stable, and thus effectively provide a suitable culture environment for them, greatly improving the convenience of culture.

[0004] The above technical solution can effectively adjust and control the temperature and light according to the needs of different cells, so as to meet different culture requirements, without additional equipment, effectively reducing the culture cost, and greatly improving the overall flexibility and convenience. However, multiple identical cell culture media are required to observe the culture of multiple groups of cells under different light conditions. The number of culture media used is large, and the operation steps are cumbersome. It is not convenient to observe the culture of multiple groups of adherent cells with the same culture medium. Therefore, we propose an adherent cell culture medium. Summary of the Utility Model

[0005] The utility model is proposed to solve the shortcomings existing in the prior art, and provides an adherent cell culture medium, which solves the problem that it is not convenient to observe the culture of multiple groups of adherent cells with the same culture medium.

[0006] In order to achieve the above purpose, the utility model adopts the following technical scheme:

[0007] An adherent cell culture medium, comprising a housing, a partition is arranged inside the housing, a cavity is arranged between the partition and the housing, the number of the cavities is four, a culture dish is arranged inside the cavity, a solid culture medium is arranged at the inner bottom of the culture dish, a pop-up component is arranged between the culture dish and the housing, the pop-up component is used for popping up the culture dish, and an adjustable light-shielding component is arranged on the upper side of the housing, and the adjustable light-shielding component is used for performing different light-shielding adjustments on the culture dish inside the cavity.

[0008] Through the above technical solution, the adherent cells to be cultured are placed on the solid culture medium in the culture dish, then the culture dish is inserted into the cavity, and then the pop-up component is used to fix between the culture dish and the housing. Then, the adjustable light-shielding component is covered on the housing to perform different light-shielding adjustments on different cavities, so as to provide an adherent cell culture with different illuminations for the same culture medium, which is convenient for observing cell growth.

[0009] Preferably, the pop-up component includes a threaded rod, the threaded rod is arranged on the lower side of the culture dish, a nut is rotatably connected to the lower side of the housing, the nut is threadedly connected with the threaded rod, and a plurality of springs are arranged between the culture dish and the housing.

[0010] Through the above technical solution, the culture dish is installed into the cavity, then the threaded rod passes through the lower side of the housing and abuts against the upper side of the nut. The nut is rotated, and the nut moves threadedly on the threaded rod. The threaded rod drives the culture dish to press the spring. When the culture dish is separated, the rebounding force of the spring provides a function of pushing and popping up the culture dish.

[0011] Preferably, the adjustable light-shielding component includes a transparent cover plate, the transparent cover plate is arranged on the upper side of the housing, a plurality of insertion slots are arranged on the outer side of the transparent cover plate, a plurality of light-shielding plates are inserted into the insertion slots, and a sealing ring is arranged on the lower side of the transparent cover plate.

[0012] Through the above technical solution, different numbers of light-shielding plates are inserted into the insertion slots on the outer side of the transparent cover plate according to the light-shielding requirements for performing different light-shielding operations on the light in the culture dish. At the same time, the transparent cover plate cooperates with the sealing ring to be hermetically clamped on the upper side of the housing, so as to provide a function of adjustable light-shielding requirements.

[0013] Preferably, a plurality of air holes I are arranged on the outer side of the sealing ring, air holes II are arranged on the outer side of the housing, air holes III are arranged on the outer side of the culture dish, the air holes II and the air holes III are arranged in a communicating structure, and a breathable diaphragm is arranged in the air holes III.

[0014] Through the above technical solution, the air hole two and the air hole three are arranged in a communicating structure and cooperate with the breathable diaphragm. Rotate the sealing ring so that the air hole one on its outer side faces the air hole two, which can provide a function of air intake and ventilation for the culture dish. On the contrary, when the air hole one and the air hole two intersect, a closed state can be provided for the culture dish.

[0015] Preferably, a support ring is provided on the lower side of the housing.

[0016] Through the above technical solution, the support ring is used to provide a supporting function for the housing.

[0017] In summary, for this utility model, the adherent cells to be cultured are placed on the solid medium in the culture dish, then the culture dish is inserted into the cavity, and then the pop-up component is used to fix between the culture dish and the housing. Then, according to the light shielding requirements, different numbers of light shielding plates are inserted into the insertion slots on the outer side of the transparent cover plate to perform different light shielding operations on the light in the culture dish. At the same time, the transparent cover plate cooperates with the sealing ring and can be hermetically clamped on the upper side of the housing, so as to provide adherent cell culture with different lights on the same culture medium, which is convenient for observing cell growth.

[0018] For this utility model, the culture dish is installed into the cavity, then the threaded rod passes through the lower side of the housing and is placed above the nut and abuts against it. Rotate the nut, the nut moves in a threaded manner on the threaded rod, and the threaded rod drives the culture dish to press down the spring. When the culture dish is separated, the rebounding force of the spring provides a function of pushing the culture dish out.

[0019] Through the above technical solution, the air hole two and the air hole three are arranged in a communicating structure and cooperate with the breathable diaphragm. Rotate the sealing ring so that the air hole one on its outer side faces the air hole two, which can provide a function of air intake and ventilation for the culture dish. On the contrary, when the air hole one and the air hole two intersect, a closed state can be provided for the culture dish. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 is an exploded structural schematic diagram of the present utility model;

[0021] Figure 2 is a front axonometric structural schematic diagram of the present utility model;

[0022] Figure 3 is a front cut-away axonometric structural schematic diagram of the present utility model.

[0023] In the figure: 1, housing; 2, partition; 3, culture dish; 4, solid medium; 5, threaded rod; 6, nut; 7, spring; 8, transparent cover plate; 9, insertion slot; 10, light shielding plate; 11, sealing ring; 12, air hole one; 13, air hole two; 14, air hole three; 15, breathable diaphragm; 16, support ring. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0024] Next, in combination with the accompanying drawings in the embodiments of the present utility model, the technical solutions in the embodiments of the present utility model will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present utility model, rather than all the embodiments.

[0025] As Figures 1-3 shown, an adherent cell culture medium includes a housing 1. A partition 2 is arranged inside the housing 1. A cavity is arranged between the partition 2 and the housing 1. The number of cavities is four. Culture dishes 3 are arranged inside the cavities. A solid culture medium 4 is arranged at the inner bottom of the culture dishes 3. A support ring 16 is arranged on the lower side of the housing 1.

[0026] A pop-up component is arranged between the culture dish 3 and the housing 1. The pop-up component is used to pop up the culture dish 3. The pop-up component includes a threaded rod 5. The threaded rod 5 is arranged on the lower side of the culture dish 3. The lower side of the housing 1 is rotatably connected with a nut 6. The nut 6 is threadedly connected with the threaded rod 5. A plurality of springs 7 are arranged between the culture dish 3 and the housing 1.

[0027] An adjustable light-shielding component is arranged on the upper side of the housing 1. The adjustable light-shielding component is used to perform different light-shielding adjustments on the culture dish 3 inside the cavity. The adjustable light-shielding component includes a transparent cover plate 8. The transparent cover plate 8 is arranged on the upper side of the housing 1. A plurality of insertion slots 9 are arranged on the outer side of the transparent cover plate 8. A plurality of light-shielding plates 10 are inserted into the insertion slots 9. A sealing ring 11 is arranged on the lower side of the transparent cover plate 8. A plurality of air holes one 12 are arranged on the outer side of the sealing ring 11. An air hole two 13 is arranged on the outer side of the housing 1. An air hole three 14 is arranged on the outer side of the culture dish 3. The air hole two 13 and the air hole three 14 are arranged in a communicating structure. An air-permeable diaphragm 15 is arranged inside the air hole three 14.

[0028] In this embodiment, the adherent cells to be cultured are placed on the solid culture medium 4 inside the culture dish 3. Then the culture dish 3 is installed into the cavity. Then the threaded rod 5 passes through the lower side of the housing 1 and is placed on the upper side of the nut 6 and abuts against it. Rotate the nut 6. The nut 6 moves the threaded rod 5 in a threaded manner. The threaded rod 5 drives the culture dish 3 to press down the spring 7. Then, according to the light-shielding requirements, different numbers of light-shielding plates 10 are inserted into the insertion slots 9 on the outer side of the transparent cover plate 8 to perform different light-shielding operations on the light in the culture dish 3. At the same time, the transparent cover plate 8 cooperates with the sealing ring 11 to be hermetically clamped on the upper side of the housing 1.

[0029] In this embodiment, the air hole two 13 and the air hole three 14 are arranged in a communicating structure and cooperate with the air-permeable diaphragm 15. Rotate the sealing ring 11 so that the air holes one 12 on its outer side are opposite to the air hole two 13, and air intake and ventilation can be performed inside the culture dish 3. On the contrary, when the air holes one 12 and the air hole two 13 are staggered, a closed state can be provided inside the culture dish 3.

[0030] The above are only the preferred specific embodiments of the present utility model, but the protection scope of the present utility model is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present utility model, according to the technical solution of the present utility model and its inventive concept, makes equivalent substitutions or changes, and should be covered by the protection scope of the present utility model.

Claims

1. An adherent cell culture medium, characterized in that The invention comprises a shell (1), wherein a partition (2) is arranged inside the shell (1), a cavity is arranged between the partition (2) and the shell (1), the number of the cavities is four, a culture dish (3) is arranged inside the cavity, a solid culture medium (4) is arranged at the inner bottom of the culture dish (3), a pop-up component is arranged between the culture dish (3) and the shell (1), the pop-up component is used to pop up the culture dish (3), and an adjustable shading component is arranged on the upper side of the shell (1), the adjustable shading component is used to perform different shading adjustments on the culture dish (3) in the cavity.

2. The adherent cell culture medium according to claim 1, characterized in that: The ejection assembly comprises a threaded rod (5), the threaded rod (5) being arranged on the lower side of the culture dish (3), a nut (6) being rotatably connected to the lower side of the shell (1), the nut (6) being threadably connected to the threaded rod (5), and a plurality of springs (7) being arranged between the culture dish (3) and the shell (1).

3. The adherent cell culture medium according to claim 1, characterized in that: The adjustable shading assembly comprises a transparent cover plate (8), the transparent cover plate (8) being arranged on the upper side of the housing (1), a plurality of plug-in slots (9) being arranged on the outer side of the transparent cover plate (8), a plurality of shading plates (10) being plugged into the plug-in slots (9), and a sealing ring (11) being arranged on the lower side of the transparent cover plate (8).

4. The adherent cell culture medium according to claim 3, characterized in that: The outer side of the sealing ring (11) is provided with a plurality of air holes one (12), the outer side of the shell (1) is provided with an air hole two (13), and the outer side of the culture dish (3) is provided with an air hole three (14). The air holes two (13) and the air holes three (14) are arranged in a connected structure, and an air permeable membrane (15) is arranged inside the air hole three (14).

5. The adherent cell culture medium according to claim 1, characterized in that: A support ring (16) is provided on the lower side of the housing (1).

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