Tissue and cell co-culture device

Abstract

The utility model discloses a tissue and cell co-culture device, which comprises a culture container, a culture container, a cell culture device and a cell culture device, wherein the lower end of the culture container is step-shaped; a sealing cover is mounted on the upper side of the culture container; a stirring mechanism is mounted on the inner side of the sealing cover; a partition culture frame is mounted on the inner side of the culture container, the partition culture frame comprises a mounting ring, connecting rods are mounted at the lower end of the mounting ring at equal angles, and partition rings are mounted at the lower ends of the connecting rods. The utility model belongs to the technical field of experimental instruments, and achieves the technical effects that the homogenate is attached to the surfaces of cells after being precipitated, so that the absorption of the cells to nutrient substances is hindered, and the growth speed and state of the cells are influenced; the homogenate is prevented from precipitating in a culture container to form a thick layer; and the problems that the contact between the cells and the tissue homogenate is not uniform enough, the interaction complexity is difficult to simulate completely, and the experimental results are inconsistent are solved.

Description

Technical Field

[0001] The utility model relates to the technical field of experimental instruments, and particularly relates to a device for co-culturing tissues and cells. Background Art

[0002] Co-culturing tissue homogenate with cells is a technical method for simulating the in-vivo environment in vitro to conduct cell biology research. Its research significance is very important. Tissue homogenate contains various cell types, extracellular matrix components, growth factors, hormones and other bioactive molecules in tissues. Co-culturing with cells can better simulate the complex environment in which cells are located in vivo, and helps to study the behavior and function of cells under more physiological conditions. In tissue homogenate, different types of cells can come into contact with each other and conduct direct cell-cell communication, such as the formation of cell junctions and the transmission of cell signals, which is very important for studying the interaction between cells. The extracellular matrix components in tissue homogenate can provide support for cell attachment and growth, and at the same time affect cell differentiation and function. Proteins in the extracellular matrix, such as collagen and fibrin, play important roles in cell morphology, migration, proliferation and differentiation. Tissue homogenate contains abundant growth factors and hormones, and these bioactive molecules can affect cell growth, proliferation, differentiation and survival, which is crucial for studying cell physiological and pathological processes. Using tissue homogenate to co-culture with cells can study the molecular mechanism of disease occurrence and development.

[0003] Tissue homogenate contains key nutrients required for cell growth, and long-term static placement of the homogenate will produce precipitates. These precipitates suspended in the culture medium are very helpful for cell growth and induction. Once they precipitate and adhere to the cell surface, a covering will be formed in the cell culture container, which will hinder the cells from absorbing these nutrients, thereby affecting the growth rate and state of the cells. If a thick layer of precipitate is formed in the culture container, it will limit the transfer of oxygen, resulting in the cells being unable to obtain enough oxygen for aerobic respiration, thereby affecting cell energy metabolism and growth. The precipitate will also hinder the excretion of metabolic wastes, resulting in the accumulation of wastes around the cells, affecting the normal respiration and metabolism of the cells. The contact between cells and tissue homogenate is not uniform enough, and the complexity of the interaction is difficult to fully simulate, resulting in different experimental results. Summary of the Utility Model

[0004] Therefore, the utility model provides a device for co-culturing tissues and cells to solve the above problems in the prior art.

[0005] In order to achieve the above object, the utility model provides the following technical solutions:

[0006] According to the first aspect of the utility model, a device for co-culturing tissues and cells includes:

[0007] A culture container, the lower end of which is stepped;

[0008] A cover is installed on the upper side of the culture container for sealing the opening of the culture container;

[0009] A stirring mechanism is installed inside the cover for stirring the tissue homogenate;

[0010] A partitioned culture rack is installed inside the culture container for increasing the cell growth area, where:

[0011] The partitioned culture rack includes a mounting ring, the lower end of which is equiangularly installed with connecting rods, and the lower end of the connecting rods is installed with a partition ring for cell proliferation. A cell proliferation platform is formed inside the culture container by using the partition ring.

[0012] Further, the stirring mechanism includes a driving rod, the upper end of which is connected to the output end of the motor, and a mounting rod is installed on the outer side of the lower end of the driving rod, and a stirring rod is installed inside the mounting rod.

[0013] Further, the longitudinal section of the stirring rod is in an "M" shape.

[0014] Further, a groove structure is formed inside the mounting rod, and the stirring rod is slidably connected to the groove structure.

[0015] Further, a magnet is installed inside the groove structure of the mounting rod, and a metal block corresponding to the magnet is arranged on the outer side of the stirring rod.

[0016] Further, a positioning block is installed on the outer side of the lower end of the cover, and a positioning ring installed on the outer side of the culture container is correspondingly arranged at the lower end of the positioning block.

[0017] Further, a convex structure is arranged at the upper end of the positioning ring, and the convex structure is snap-fitted with the positioning block.

[0018] The utility model has the following advantages:

[0019] The utility model solves the problems that the homogenate precipitates and adheres to the cell surface, hindering the cells from absorbing nutrients, thereby affecting the growth rate and state of the cells; avoids the formation of a thick layer of precipitated homogenate in the culture container; and solves the problem that the contact between the cells and the tissue homogenate is not uniform enough, and the complexity of the interaction is difficult to fully simulate, resulting in inconsistent experimental results. Description of the Drawings

[0020] Figure 1 It is a front sectional view of a device for co-culturing tissues and cells provided by some embodiments of the utility model.

[0021] Figure 2 A perspective view of a partition culture rack of a device for co - culturing tissues and cells provided by some embodiments of the present utility model.

[0022] Figure 3 A top view of the connection between a stirring rod and a mounting rod of a device for co - culturing tissues and cells provided by some embodiments of the present utility model.

[0023] In the figure: 1, culture container; 2, cover; 3, positioning block; 4, positioning ring; 5, stirring mechanism; 501, driving rod; 502, mounting rod; 503, stirring rod; 504, magnet; 6, partition culture rack; 601, mounting ring; 602, connecting rod; 603, partition ring. Detailed implementation manners

[0024] The following specific embodiments illustrate the implementation manners of the present utility model. Those skilled in the art can easily understand other advantages and effects of the present utility model from the content disclosed in this specification. Obviously, the described embodiments are part of the embodiments of the present utility model, rather than all of them. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present utility model.

[0025] Embodiment 1

[0026] As Figures 1 to 3 shown, a device for co - culturing tissues and cells in the first - aspect embodiment of the present utility model includes a culture container 1. The lower end of the culture container 1 is in a stepped shape, which can enable different steps to concentrate a certain volume of tissue homogenate, avoiding the large - scale accumulation of tissue homogenate on the same plane, thereby preventing a large number of cells from proliferating and growing, and avoiding the accumulation of waste around the cells, which affects the normal respiration and metabolism of the cells.

[0027] A cover 2 is installed on the upper side of the culture container 1 to seal the opening of the culture container 1 and prevent the external environment from contacting the culture environment inside the culture container 1. A positioning block 3 is installed on the outer side of the lower end of the cover 2, and a positioning ring 4 installed on the outer side of the culture container 1 is correspondingly arranged at the lower end of the positioning block 3. A convex structure is provided at the upper end of the positioning ring 4, and this convex structure forms a snap - fit connection with the positioning block 3, which can position and install the cover 2 and the culture container 1.

[0028] A stirring mechanism 5 is installed inside the cover 2 for stirring the tissue homogenate, where:

[0029] The stirring mechanism 5 includes a driving rod 501. The upper end of the driving rod 501 is connected to the output end of the motor. A mounting rod 502 is installed on the outer side of the lower end of the driving rod 501. A stirring rod 503 is installed inside the mounting rod 502, and the longitudinal section of the stirring rod 503 is in an "M" shape.

[0030] Specifically, when the driving rod 501 rotates, it can drive the stirring rod 503 to rotate and stir the tissue homogenate in the culture container 1. By using the fact that the longitudinal section of the stirring rod 503 is in an "M" shape, it can adapt to the stepped setting at the lower end of the culture container 1 and stir the tissue homogenate at the bottom, avoiding a large amount of precipitation of the sediment in the tissue homogenate locally, so as to avoid the formation of a covering on the cell surface.

[0031] A partitioned culture rack 6 is installed inside the culture container 1 for increasing the cell growth area, where:

[0032] The partitioned culture rack 6 includes a mounting ring 601. The lower end of the mounting ring 601 is equiangularly installed with connecting rods 602, and the lower end of the connecting rod 602 is installed with a partition ring 603 for cell proliferation. By using the partition ring 603 to form a cell proliferation platform inside the culture container 1, a precipitation bearing area for the sediment in the multi-layer tissue homogenate can be provided at the deeper step outside the culture container 1, thus facilitating cell growth.

[0033] Embodiment 2

[0034] As Figure 1 and Figure 2 shown, a device for co-culturing tissues and cells includes all the contents of Embodiment 1. In addition, a groove-like structure is formed inside the mounting rod 502, and the stirring rod 503 is slidably connected to the groove-like structure.

[0035] The technical effect achieved by the above embodiment is that after the driving rod 501 rotates, the centrifugal force generated by the rotation is used to move the stirring rod 503 outward. At the same time, when removing the cover 2, it is convenient to move the stirring rod 503 inward, thus avoiding a position conflict with the partition ring 603.

[0036] Embodiment 3

[0037] As Figure 1 and Figure 2 shown, a device for co-culturing tissues and cells includes all the contents of Embodiment 2. In addition, a magnet 504 is installed in the groove-like structure of the mounting rod 502, and a metal block corresponding to the magnet 504 is arranged on the outer side of the stirring rod 503.

[0038] The technical effect achieved by the above embodiment is that the stirring rod 503 can be positioned, and the adsorption effect of the magnet 504 is lost when separated, avoiding automatic reset. Only after the driving rod 501 rotates, the stirring rod 503 can move outward and fit with the magnet 504.

Claims

1. An apparatus for co-culturing tissues and cells, characterized in that, Comprising: A culture container (1), the lower end of the culture container (1) being stepped; A cover (2) is installed on the upper side of the culture container (1) for sealing the opening of the culture container (1); A stirring mechanism (5) is installed inside the cover (2) for stirring the tissue homogenate; A partition culture rack (6) is installed inside the culture container (1) for increasing the cell growth area, wherein: The partition culture rack (6) includes a mounting ring (601), the lower end of the mounting ring (601) is equiangularly provided with connecting rods (602), and the lower end of the connecting rod (602) is provided with a partition ring (603) for cell proliferation, and a cell proliferation platform is formed inside the culture container (1) by using the partition ring (603).

2. The device for co-culturing tissue and cells according to claim 1, characterized in that, The stirring mechanism (5) includes a driving rod (501), the upper end of the driving rod (501) is connected to the output end of the motor, the outer side of the lower end of the driving rod (501) is provided with a mounting rod (502), and a stirring rod (503) is installed inside the mounting rod (502).

3. The device for co-culturing tissues and cells according to claim 2, characterized in that, The longitudinal section of the stirring rod (503) is in an "M" shape.

4. The device for co-culturing tissues and cells according to claim 2, characterized in that, A groove structure is formed inside the mounting rod (502), and the stirring rod (503) is slidably connected to the groove structure.

5. The device for co-culturing tissue and cells according to claim 4, characterized in that, A magnet (504) is further installed in the groove structure of the mounting rod (502), and a metal block corresponding to the magnet (504) is arranged on the outer side of the stirring rod (503).

6. The device for co-culturing tissue and cells according to claim 1, wherein A positioning block (3) is installed on the outer side of the lower end of the cover (2), and a positioning ring (4) installed on the outer side of the culture container (1) is correspondingly arranged at the lower end of the positioning block (3).

7. The device for co-culturing tissue and cells according to claim 6, characterized in that, A convex structure is arranged at the upper end of the positioning ring (4), and the convex structure is snap-connected to the positioning block (3).

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