Oscillating device for DNA methylation test

Abstract

The utility model relates to the technical field of test devices, and discloses an oscillation device for a DNA methylation test, which comprises an oscillation shell and a test tube, an oscillation seat is arranged on the inner side of the oscillation shell, positioning grooves are uniformly formed in the upper surface of the oscillation seat, and the lower end of the test tube is movably connected with the positioning grooves. A driving motor is controlled to work, a first cam can be driven to rotate through a first connecting rod, so that a vibration plate and a vibration seat are driven to vibrate, a test tube on the inner side of a positioning groove is further driven to vibrate up and down, and a second cam can be driven to rotate in a shell through combination of a first bevel gear, a second connecting rod and a second bevel gear; through combination of a connecting block, a first guide rod and a first spring, a vibration block can be driven to move leftwards and rightwards, through combination of a second sliding block, a second sliding groove and a second spring, when the vibration block moves rightwards, a vibration seat can be knocked, so that a test tube on the inner side of a positioning groove is driven to vibrate leftwards and rightwards, and the vibration effect of the test tube is further improved.

Description

Technical Field

[0001] This utility model relates to the field of experimental device technology, specifically to a shaking device for DNA methylation testing. Background Technology

[0002] In DNA methylation assays, mixing is a common procedure. After the reagents have reacted, the operator needs to hold the sample tube and shake it to ensure that the solution is fully and thoroughly mixed.

[0003] A search revealed that CN214781770U discloses a shaking device for DNA methylation testing. This device drives a rotating rod to rotate back and forth, which in turn drives a cam and an arc-shaped rod to rotate simultaneously. Because the other side of the arc-shaped rod is slidably connected inside the U-shaped rod, the U-shaped rod moves back and forth. At this time, the other side of the arc-shaped rod slides inside the U-shaped rod. Due to the integrated structural design, the entire shaking frame shakes.

[0004] In the process of realizing this utility model, the inventors discovered that at least the following problems in the prior art have not been solved. In the above case, during use, the rotating rod drives the cam and the arc rod to rotate back and forth, thereby driving the entire oscillation frame to oscillate back and forth. The oscillation direction is unidirectional, resulting in limited oscillation effect.

[0005] Therefore, we propose a shaking device for DNA methylation assays that can shake the test tube up and down and left and right. Utility Model Content

[0006] The purpose of this invention is to provide a shaking device for DNA methylation assay, which solves the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution: a shaking device for a DNA methylation test, comprising a shaking shell and a test tube, wherein a shaking seat is provided on the inner side of the shaking shell, and a positioning groove is uniformly formed on the upper surface of the shaking seat, the lower end of the test tube is movably connected to the positioning groove, and a shaking plate is horizontally arranged on the bottom side of the shaking seat; a first shaking mechanism for vertical vibration of the shaking seat, the first shaking mechanism comprising a first connecting rod and a drive motor, a first cam fixedly mounted on both sides of the first connecting rod, the shaft end of the drive motor fixedly connected to one end of the first connecting rod, and a first bevel gear fixedly mounted on the end of the first connecting rod away from the drive motor; a second shaking mechanism for horizontal vibration of the shaking seat, the second shaking mechanism comprising a second connecting rod and a shaking block, wherein the middle part of one side of the shaking shell is fixedly mounted on the second connecting rod; a second shaking mechanism for horizontal vibration of the shaking seat, the second shaking mechanism comprising a second connecting rod and a shaking block; and a third shaking mechanism for vertical vibration of the shaking seat. A housing is fixedly installed. A second bevel gear is fixedly installed at the bottom end of the second connecting rod. The first bevel gear meshes with the second bevel gear. A second cam is fixedly installed through and on one end of the second connecting rod located inside the housing. A connecting block is slidably connected to the inside of the housing. A first guide rod is horizontally fixedly installed on the side of the connecting block away from the second cam. A vibration block is fixedly installed on one end of the first guide rod located outside the housing. The vibration block is movably connected to one side of the oscillating seat. A first spring is provided on the side of the connecting block away from the second cam. Second sliders are symmetrically installed on both sides of the lower surface of the oscillating seat. Second grooves are horizontally opened on both sides of the upper surface of the oscillating plate. The second slider on the bottom side of the oscillating seat is slidably connected to the second grooves on the upper surface of the oscillating plate. A second spring is provided on the side of the second slider near the drive motor.

[0008] As an optional solution to the technical solution of this application, a fixing frame is detachably installed above the oscillating seat, and the bottom side of the fixing frame abuts against the top of the test tube.

[0009] As an optional solution to the technical solution of this application, the lower part of the inner wall of both sides of the oscillating shell is provided with a first sliding groove vertically and symmetrically, and the two sides of the oscillating plate are symmetrically installed with a first slider, and the first sliders on both sides of the oscillating plate are slidably connected to the first sliding grooves on the inner wall of both sides of the oscillating shell.

[0010] As an optional solution to the technical solution of this application, a second guide rod is horizontally fixedly installed inside the second slide groove. The second guide rod horizontally passes through the second slider and is slidably connected to the second slider. The second spring is sleeved on the outside of the second guide rod.

[0011] As an optional solution to the technical solution of this application, the first guide rod horizontally penetrates the housing and is slidably connected to the housing, and the first spring is sleeved on the outside of the first guide rod.

[0012] As an optional solution to the technical solution of this application, the second connecting rod is rotatably connected to the housing via a bearing, the first connecting rod is rotatably connected to the lower part of the inner wall on both sides of the oscillating housing via a bearing, and the drive motor is fixedly connected to the outside of the oscillating housing.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: By controlling the operation of the drive motor, the first cam can be rotated through the first connecting rod, thereby causing the vibrating plate and the vibrating seat to vibrate, and further causing the test tube inside the positioning groove to vibrate up and down. Through the combination of the first bevel gear, the second connecting rod and the second bevel gear, the second cam can be rotated in the housing. Through the combination of the connecting block, the first guide rod and the first spring, the vibrating block can be moved left and right. Through the combination of the second slider, the second slide groove and the second spring, when the vibrating block moves to the right, it can strike the vibrating seat, thereby causing the test tube inside the positioning groove to vibrate left and right, further improving the vibration effect of the test tube. Attached Figure Description

[0014] Other features, objects, and advantages of this invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0015] Figure 1 This is a front view of a shaking device for DNA methylation testing according to the present invention;

[0016] Figure 2 This is a schematic diagram of the internal structure of a shaking device for DNA methylation testing according to this utility model.

[0017] In the diagram: 1. Vibrating shell; 11. Vibrating seat; 12. Positioning groove; 13. Test tube; 14. Vibrating plate; 15. First connecting rod; 16. First cam; 17. Drive motor; 18. First bevel gear; 2. Shell; 21. Second connecting rod; 22. Second cam; 23. Second bevel gear; 24. Connecting block; 25. First guide rod; 26. Vibrating block; 27. First spring; 3. Second slide groove; 31. Second slider; 32. Second spring; 33. Second guide rod; 34. First slide groove; 35. First slider; 36. Fixing frame. Detailed Implementation

[0018] Please see Figures 1-2This utility model provides a technical solution: a shaking device for DNA methylation assay, comprising a shaking shell 1 and a test tube 13. A shaking seat 11 is provided inside the shaking shell 1. Positioning grooves 12 are evenly distributed on the upper surface of the shaking seat 11. The lower end of the test tube 13 is movably connected to the positioning grooves 12. A fixing frame 36 is detachably installed above the shaking seat 11, with its bottom side abutting against the top of the test tube 13. A shaking plate 14 is horizontally arranged on the bottom side of the shaking seat 11. A first shaking mechanism is used for the up-and-down vibration of the shaking seat 11. The first shaking mechanism includes a first connecting rod 15 and a drive motor 17. First cams 16 are fixedly installed on both sides of the connecting rod 15. The shaft end of the drive motor 17 is fixedly connected to one end of the first connecting rod 15. First grooves 34 are vertically and symmetrically opened on the lower part of the inner walls on both sides of the oscillating shell 1. First sliders 35 are symmetrically installed on both sides of the oscillating plate 14. The first sliders 35 on both sides of the oscillating plate 14 are slidably connected to the first grooves 34 on the inner walls on both sides of the oscillating shell 1. The first connecting rod 15 is rotatably connected to the lower part of the inner walls on both sides of the oscillating shell 1 through bearings. The drive motor 17 is fixedly connected to the outside of the oscillating shell 1. A first bevel gear 18 is fixedly installed on the end of the first connecting rod 15 away from the drive motor 17.

[0019] In this technical solution, the bottom end of the test tube 13 is inserted into the positioning groove 12 opened on the upper surface of the shaking base 11, and then the fixing bracket 36 is fixed above the shaking base 11 by bolts, which can limit and fix the top end of the test tube 13. The corresponding controller controls the drive motor 17 to work, which can drive the first connecting rod 15 to rotate, thereby driving the first cam 16 to rotate on the bottom side of the shaking plate 14. The first slider 35 is slidably connected to the first sliding groove 34. When the convex end of the first cam 16 abuts against the bottom side of the shaking plate 14, it can push the shaking plate 14 to move upward. When the convex end of the first cam 16 rotates away from the shaking plate 14, the shaking plate 14 moves downward and resets under the action of gravity. Further, the first cam 16 reciprocates, which can drive the shaking plate 14 and the shaking base 11 to vibrate up and down, thereby driving the test tube 13 inside the positioning groove 12 to vibrate up and down.

[0020] In this embodiment, the second oscillation mechanism is used for the left and right vibration of the oscillation seat 11. The second oscillation mechanism includes a second connecting rod 21 and a vibration block 26. A housing 2 is fixedly installed in the middle of one side of the oscillation housing 1. A second bevel gear 23 is fixedly installed at the bottom end of the second connecting rod 21. The first bevel gear 18 meshes with the second bevel gear 23. The second connecting rod 21 is rotatably connected to the housing 2 through a bearing. A second cam 22 is fixedly installed through and inside the housing 2 at one end of the second connecting rod 21.

[0021] In this technical solution, when the first connecting rod 15 drives the first cam 16 to rotate, it can drive the first bevel gear 18 to rotate. Through the meshing of the first bevel gear 18 and the second bevel gear 23, the second bevel gear 23 can drive the second cam 22 to rotate inside the housing 2 via the second connecting rod 21.

[0022] In this embodiment, a connecting block 24 is slidably connected to the inner side of the housing 2. A first guide rod 25 is horizontally fixedly installed on the side of the connecting block 24 away from the second cam 22. The first guide rod 25 horizontally penetrates the housing 2 and is slidably connected to the housing 2. A vibration block 26 is fixedly installed at the end of the first guide rod 25 located on the outer side of the housing 2. The vibration block 26 is movably connected to one side of the oscillating seat 11. A first spring 27 is provided on the side of the connecting block 24 away from the second cam 22. The first spring 27 is sleeved on the outer side of the first guide rod 25. Second sliders 31 are symmetrically installed on both sides of the lower surface of the oscillating seat 11. Second grooves 3 are horizontally opened on both sides of the upper surface of the oscillating plate 14. The second slider 31 on the bottom side of the oscillating seat 11 is slidably connected to the second groove 3 on the upper surface of the oscillating plate 14. A second spring 32 is provided on the side of the second slider 31 near the drive motor 17. A second guide rod 33 is horizontally fixedly installed inside the second groove 3. The second guide rod 33 horizontally penetrates the second slider 31 and is slidably connected to the second slider 31. The second spring 32 is sleeved on the outer side of the second guide rod 33.

[0023] In this technical solution, the connecting block 24 is slidably connected to the housing 2. When the protruding end of the second cam 22 abuts against the outside of the connecting block 24, it can push the connecting block 24 to move to the right. The first guide rod 25 can push the vibrating block 26 to move to the right and strike the vibrating seat 11. The second slider 31 is slidably connected to the second slide groove 3, which can push the vibrating seat 11 to move to the right. When the protruding end of the second cam 22 rotates away from the connecting block 24, the elastic force of the first spring 27 can push the connecting block 24 to move to the left and reset. The first guide rod 25 further pulls the vibrating block 26 to move to the left and reset. At the same time, the elastic force of the second spring 32 can push the vibrating seat 11 to move to the left and reset. The vibrating block 26 repeatedly strikes the vibrating seat 11, which can make the vibrating seat 11 vibrate back and forth, thereby driving the test tube 13 inside the positioning groove 12 to vibrate left and right, further improving the vibration effect of the test tube 13.

[0024] In the use of a shaking device for a DNA methylation test, the bottom end of the test tube 13 is inserted into the positioning groove 12 on the upper surface of the shaking base 11. Then, the fixing bracket 36 is fixed above the shaking base 11 by bolts, which can limit and fix the top end of the test tube 13. The corresponding controller controls the drive motor 17 to work, which can drive the first connecting rod 15 to rotate, thereby driving the first cam 16 to rotate on the bottom side of the shaking plate 14. The first slider 35 is slidably connected to the first slide groove 34. When the convex end of the first cam 16 abuts against the bottom side of the shaking plate 14, it can push the shaking plate 14 to move upward. When the convex end of the first cam 16 rotates away from the shaking plate 14, the shaking plate 14 moves downward and resets under the action of gravity. Further, the first cam 16 reciprocates, which can drive the shaking plate 14 and the shaking base 11 to vibrate up and down, thereby driving the test tube 13 inside the positioning groove 12 to vibrate up and down. During the process of the first connecting rod 15 driving the first cam 16 to rotate, it can drive the first bevel gear 18 to rotate, which can drive the first bevel gear 18 to rotate. Gear 18 meshes with the second bevel gear 23. During the rotation of the second bevel gear 23, the second cam 22 can be driven to rotate inside the housing 2 via the second connecting rod 21. It is slidably connected to the housing 2 via the connecting block 24. When the convex end of the second cam 22 abuts against the outside of the connecting block 24, it can push the connecting block 24 to move to the right. This pushes the vibrating block 26 to move to the right and strike the vibrating seat 11 via the first guide rod 25. The second slider 31 is slidably connected to the second slide groove 3, which can push the vibrating seat 11 to move to the right. When the convex end of the second cam 22 rotates away from the connecting block 24, the connecting block 24 can be pushed to the left and reset by the elastic force of the first spring 27. Furthermore, the first guide rod 25 pulls the vibrating block 26 to the left and reset. At the same time, the vibrating seat 11 can be pushed to the left and reset by the elastic force of the second spring 32. Furthermore, the vibrating block 26 repeatedly strikes the vibrating seat 11, which can make the vibrating seat 11 vibrate back and forth, thereby driving the test tube 13 inside the positioning groove 12 to vibrate left and right, further improving the vibration effect of the test tube 13.

Claims

1. A shaking device for a DNA methylation assay, comprising a shaking shell (1) and a test tube (13), wherein a shaking seat (11) is provided on the inner side of the shaking shell (1), and a positioning groove (12) is uniformly formed on the upper surface of the shaking seat (11), and the lower end of the test tube (13) is movably connected to the positioning groove (12), characterized in that, A oscillating plate (14) is horizontally arranged on the bottom side of the oscillating seat (11); First oscillation mechanism: used for the up and down vibration of the oscillation seat (11). The first oscillation mechanism includes a first connecting rod (15) and a drive motor (17). First cams (16) are fixedly installed on both sides of the first connecting rod (15). The shaft end of the drive motor (17) is fixedly connected to one end of the first connecting rod (15). A first bevel gear (18) is fixedly installed on the end of the first connecting rod (15) away from the drive motor (17). Second oscillation mechanism: used for left and right vibration of the oscillation seat (11), the second oscillation mechanism includes a second connecting rod (21) and a vibrating block (26), a housing (2) is fixedly installed in the middle of one side of the oscillation housing (1), a second bevel gear (23) is fixedly installed at the bottom end of the second connecting rod (21), the first bevel gear (18) meshes with the second bevel gear (23), a second cam (22) is fixedly installed through and inside the housing (2) at one end of the second connecting rod (21), a connecting block (24) is slidably connected to the inside of the housing (2), and a first guide rod (25) is horizontally fixedly installed on the side of the connecting block (24) away from the second cam (22). A vibration block (26) is fixedly installed at one end of the first guide rod (25) located outside the housing (2). The vibration block (26) is movably connected to one side of the oscillating seat (11). A first spring (27) is provided on the side of the connecting block (24) away from the second cam (22). Second sliders (31) are symmetrically installed on both sides of the lower surface of the oscillating seat (11). Second grooves (3) are horizontally opened on both sides of the upper surface of the oscillating plate (14). The second slider (31) on the bottom side of the oscillating seat (11) is slidably connected to the second groove (3) on the upper surface of the oscillating plate (14). A second spring (32) is provided on the side of the second slider (31) near the drive motor (17).

2. The shaking device for DNA methylation assay according to claim 1, characterized in that: A fixing frame (36) is detachably installed above the oscillating seat (11), and the bottom side of the fixing frame (36) abuts against the top of the test tube (13).

3. The shaking device for DNA methylation assay according to claim 1, characterized in that: The inner walls of the two sides of the oscillating shell (1) are vertically and symmetrically provided with first sliding grooves (34), and the two sides of the oscillating plate (14) are symmetrically installed with first sliders (35). The first sliders (35) on both sides of the oscillating plate (14) are slidably connected to the first sliding grooves (34) on both sides of the inner walls of the oscillating shell (1).

4. The shaking device for DNA methylation assay according to claim 1, characterized in that: The second guide rod (33) is horizontally fixedly installed inside the second slide groove (3). The second guide rod (33) horizontally passes through the second slider (31) and is slidably connected to the second slider (31). The second spring (32) is sleeved on the outside of the second guide rod (33).

5. The shaking device for DNA methylation assay according to claim 1, characterized in that: The first guide rod (25) passes horizontally through the housing (2) and is slidably connected to the housing (2), and the first spring (27) is sleeved on the outside of the first guide rod (25).

6. The shaking device for DNA methylation assay according to claim 1, characterized in that: The second connecting rod (21) is rotatably connected to the housing (2) via a bearing, the first connecting rod (15) is rotatably connected to the lower part of the inner wall on both sides of the oscillating housing (1) via a bearing, and the drive motor (17) is fixedly connected to the outside of the oscillating housing (1).

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