Constant-distance liquid nitrogen fumigation and storage integrated device

Abstract

The utility model discloses a constant-distance liquid nitrogen fumigation and storage integrated device, and relates to the technical field of scientific research instruments. The two steps of liquid nitrogen fumigation and storage of seminal fluid can be completed in the liquid nitrogen tank, the steps of liquid nitrogen injection for balancing, fumigation height adjustment and the like are not needed, the operation steps are simplified, and the liquid nitrogen fumigation device can be suitable for liquid nitrogen tanks with different calibers. The device comprises an integrated extraction box, a carrying box and a constant-distance fumigation clamping seat, the integrated extraction box comprises a main box body, an extraction plate, a movable top plate and a handle; the main box body is of a cuboid structure with openings in the front upper surface. The extraction plate is slidably connected to an opening in the front surface of the main box body; the movable top plate is detachably connected to the opening of the upper surface of the main box body; the lower end of the handle is connected to the main box body, and the upper end forms a hanging part; the constant-distance fumigation clamping seat is hung on the lifting handle; a mounting cavity is formed in the constant-distance fumigation clamping seat; the object carrying box is located in the mounting cavity; carrying box clamping grooves are formed in the two opposite side faces of the carrying box.

Description

Technical Field

[0001] This application relates to the field of scientific research instrument technology, and in particular to an integrated device for constant-distance liquid nitrogen fumigation and storage. Background Technology

[0002] Cryopreservation is a process of preserving organelles, cells, tissues, or any other biological structures by cooling samples to extremely low temperatures. In the construction of experimental animal models, it is essential to ensure the availability and usability of germplasm cells. For male animals, sperm and semen, after proper cryopreservation, can be used almost indefinitely, providing an effective way to protect the genetic resources of important model animals. Slow freezing is a commonly used cryopreservation method in laboratories for mammals such as cynomolgus monkeys and rhesus monkeys. The semen is first mixed with a cryopreservation solution, and after pre-cooling, the mixture is placed in frozen straws. Liquid nitrogen vapor is used for cooling; when the temperature reaches below -30°C, it is placed in liquid nitrogen for storage. In some animal experiments, it is necessary to control the rate of slow freezing pre-cooling, i.e., changing the distance between the sample interface and the liquid nitrogen surface. Liquid nitrogen fumigation boxes are currently the most common equipment used in laboratories for liquid nitrogen vapor cooling. Each fumigation requires a fresh injection of liquid nitrogen for equilibration before use, and after fumigation, the liquid nitrogen must be refilled into the liquid nitrogen tank. This method is inconvenient and lacks cryopreservation capabilities. Chinese patent "A Liquid Nitrogen Fumigation Suspension Device" (Publication No.: CN220174282U) discloses a suspension device for completing liquid nitrogen fumigation in a liquid nitrogen tank. The device includes a tank opening latch and a suspension component, with space provided on the suspension component to accommodate a freezing tube and / or freezing rod. This device is fixed to the opening of the liquid nitrogen tank. Each fumigation requires ensuring the distance between the liquid nitrogen inside the tank and the tank opening, and a specially designed lid that fits the size of the suspension component is required. Furthermore, this suspension component only has a fumigation function and lacks a freezing storage function, requiring a separate storage component for storage. Utility Model Content

[0003] The embodiments of this application provide an integrated device for constant-distance liquid nitrogen fumigation and storage, which can not only complete the two steps of liquid nitrogen fumigation and storage of semen in a liquid nitrogen tank, but also eliminate the need for steps such as injecting liquid nitrogen for balancing and adjusting the fumigation height, thus simplifying the operation steps. It can also be applied to liquid nitrogen tanks of different diameters.

[0004] To achieve the above objectives, embodiments of this application provide an integrated device for constant-distance liquid nitrogen fumigation and storage, including an integrated extraction box, a container, and a constant-distance fumigation holder; the integrated extraction box includes a main body, an extraction plate, a movable top plate, and a handle; the main body is a cuboid structure with openings on both the front and top surfaces; the extraction plate is slidably connected to the opening on the front surface of the main body; the movable top plate is detachably connected to the opening on the top surface of the main body; the lower end of the handle is connected to the main body, and the upper end forms a hook; the constant-distance fumigation holder is hung on the handle; the interior of the constant-distance fumigation holder forms an installation cavity; the container is located within the installation cavity; the container is a cuboid structure with a hollowed-out front surface; container slots are provided on two opposite sides of the container.

[0005] Furthermore, the main housing includes a base plate and a left side plate, a right side plate, and a rear side plate fixed to the base plate; the inner sides of the left side plate and the right side plate are provided with main housing slots; the pull-out plate can slide up and down within the main housing slots.

[0006] Furthermore, the movable top plate and the extraction plate are connected by a snap-fit ​​mechanism.

[0007] Furthermore, the hook-on portion at the upper end of the handle includes a horizontally bent section and a vertically bent section.

[0008] Furthermore, an information card placement area is provided on the front surface of the container.

[0009] Furthermore, the information card placement area is a panel welded to the front surface of the container; the panel has an installation slot; the installation slot is used to place the information card.

[0010] Furthermore, there are multiple storage boxes; the upper surface of each storage box is provided with two storage box clips that are adapted to the storage box slots.

[0011] Furthermore, the side of the constant-distance fumigation holder is provided with a fixing ring.

[0012] Furthermore, the dimensions of the container are compatible with the dimensions of the mounting cavity of the constant-distance fumigation card holder and the inner cavity of the integrated extraction box.

[0013] Furthermore, the container is made of refrigerant-coated sheet.

[0014] This application has the following advantages over the prior art:

[0015] 1. In this embodiment, the constant-distance fumigation holder is hung on the handle of the integrated extraction box. During liquid nitrogen fumigation, the container is locked inside the constant-distance fumigation holder. When storing the items, there is no need to disassemble the constant-distance fumigation holder. Simply lift the extraction plate of the integrated extraction box, put the container in, and then close the integrated extraction box. This achieves the purpose of performing liquid nitrogen fumigation and storage in the same device to obtain frozen semen with stable quality.

[0016] 2. The embodiments of this application can realize the integration of liquid nitrogen fumigation and liquid nitrogen cryopreservation, eliminating the need for liquid nitrogen fumigation outside the storage liquid nitrogen tank, reducing operating equipment, simplifying the operation process, and the simple equipment composition and operation process allow one person to complete sperm freezing independently without the need for a special person to accompany them, thus improving the versatility of the equipment.

[0017] 3. The embodiments of this application can be used in various laboratories with suitable liquid nitrogen tank opening diameters. The device has high mobility and strong practicality. Moreover, the liquid nitrogen in the liquid nitrogen tank only needs to be kept at the normal capacity. There is no need to determine a certain capacity or distance from the tank opening, making the operation simple.

[0018] 4. The embodiments of this application can accommodate multiple storage boxes, resulting in a large storage capacity.

[0019] 5. The embodiments of this application are compatible with various semen cryopreservation carriers such as wheat straws, quartz glass tubes, and cryotubes. Attached Figure Description

[0020] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this application. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0021] Figure 1 This is a schematic diagram of the integrated extraction box in the constant-distance liquid nitrogen fumigation and storage integrated device according to an embodiment of this application;

[0022] Figure 2 This is a cross-sectional view of the main casing in the constant-distance liquid nitrogen fumigation and storage integrated device according to an embodiment of this application;

[0023] Figure 3 This is a schematic diagram of the structure of the container in the constant-distance liquid nitrogen fumigation and storage integrated device according to an embodiment of this application;

[0024] Figure 4 This is a side view of the container in the constant-distance liquid nitrogen fumigation and storage integrated device according to an embodiment of this application;

[0025] Figure 5This is a schematic diagram of the constant-distance fumigation card holder in the constant-distance liquid nitrogen fumigation and storage integrated device according to an embodiment of this application. Detailed Implementation

[0026] The technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, and not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0027] In the description of this application, it should be understood that the terms "center", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this application.

[0028] In the description of this application, it should be noted that, unless otherwise expressly specified and limited, the terms "installation", "connection" and "joining" should be interpreted broadly, for example, they can refer to a fixed connection, a detachable connection, or an integral connection; those skilled in the art can understand the specific meaning of the above terms in this application according to the specific circumstances.

[0029] The terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Therefore, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this application, unless otherwise stated, "a plurality of" means two or more.

[0030] Reference Figures 1 to 5 This application provides an integrated device for constant-distance liquid nitrogen fumigation and storage, including an integrated extraction box 1, a container 2, and a constant-distance fumigation holder 3. During liquid nitrogen fumigation, the constant-distance fumigation holder 3 is hung on the integrated extraction box 1, and the container 2 is secured within the constant-distance fumigation holder 3. During storage, the container 2 is placed directly inside the integrated extraction box 1.

[0031] Reference Figure 1 and Figure 2The integrated drawer box 1 includes a main body 11, a drawer plate 12, a movable top plate 13, and a handle 14. The main body 11 is a cuboid structure with openings on both the front and top surfaces. Specifically, the main body 11 includes a bottom plate 111 and a left side plate 112, a right side plate 113, and a rear side plate 114 fixed to the bottom plate 111. The drawer plate 12 and the rear side plate 114 are both rectangular plates with a height of 30cm and a length of 14cm. The left side plate 112 and the right side plate 113 are both rectangular plates with a height of 30cm and a length of 14.5cm. The bottom plate 111 and the movable top plate 13 are both rectangular plates with a length of 14cm and a width of 14.5cm.

[0032] Both the left side panel 112 and the right side panel 113 have inwardly opening main body slots 15 on their inner sides. The pull-out plate 12 is slidably connected to the opening on the front surface of the main body 11 and can be pulled up and down within the main body slots 15. The movable top plate 13 is connected to the opening on the upper surface of the main body 11 via a buckle 16. Specifically, the movable top plate 13 is engaged with both the pull-out plate 12 and the rear side panel 114. After opening the buckle 16, the pull-out plate 12 can be lifted and lowered to access items in the integrated pull-out box 1.

[0033] The handle 14 is 50cm long. The lower end of the handle 14 is welded to the outer side of the rear panel 114 of the main body 11, and the upper end forms a hook-up part, which includes a horizontal bending section and a vertical bending section. The bending section is fixed in the slot of the liquid nitrogen tank opening.

[0034] Reference Figure 5 The height of the constant-distance fumigation holder 3 is equal to the sum of the height of the loading chamber and the required distance above the liquid nitrogen; the specific height is determined experimentally. The constant-distance fumigation holder 3 includes four end-to-end support walls 31, the interior of which forms an installation cavity 32. The thickness of the support walls 31 is 1.7 cm, with the inner and outer walls parallel to each other, and the distance between the inner walls is 14 cm. The horizontal plane area enclosed by the inner walls is the same as the horizontal plane area of ​​the loading box. A fixing ring 33 is fixedly connected to the center of the side of the constant-distance fumigation holder 3. Before liquid nitrogen fumigation, the fixing ring 33 must be hung on the handle 14 of the integrated extraction box 1 to ensure that the constant-distance fumigation holder 3 will not drift away during liquid nitrogen fumigation.

[0035] Reference Figure 3 and Figure 4The container 2 is a cuboid structure with a perforated front surface. Specifically, the container 2 is made of a refrigerant-coated sheet with a perforated front panel and fully enclosed sides. The interior of the container 2 forms a container cavity 21. An information card placement area 22 is located on the front surface of the container 2. The information card placement area 22 is a panel with slots welded to the top and bottom edges of the perforated surface, allowing information cards to be inserted. The container cavity 21 is a cuboid with a length and width of 14cm and a height of 1.5cm. The container cavity 21 is used to place items requiring liquid nitrogen fumigation and storage; the items are placed through the perforated surface. The container 2 has a large loading capacity and is compatible with various semen cryopreservation carriers such as straws, quartz glass tubes, and cryotubes.

[0036] The container 2 has downward-facing container slots 23 on both its left and right sides. The container slots 23 are 0.5 cm wide and 1.4 cm high. Thus, when the container 2 is placed in the constant-distance fumigation holder 3, the container slots 23 can be locked into the inner side of the holder support wall 31.

[0037] To increase storage capacity, multiple storage boxes 2 are provided. The upper surface of each storage box 2 has two storage box locking blocks 24 that mate with the storage box locking slots 23. Each storage box locking block 24 is 14cm long, 0.4cm wide, and 1.4cm high. When multiple storage boxes 2 are stacked, the lower storage box locking slot 23 engages with the upper storage box 2's locking slot 23, providing a fixing function during stacking and a guiding function when storing or retrieving items from the storage cavity 21. Furthermore, this embodiment provides a suitable placement space, capable of accommodating cryogenic carriers of varying shapes and lengths, and the liquid nitrogen fumigation and storage environment offers good sealing, minimizing the chance of the cryogenic carriers falling out.

[0038] The usage process of this application embodiment is as follows:

[0039] The integrated extraction box 1 is the main part for the fumigation and storage of semen in liquid nitrogen. During the liquid nitrogen fumigation and storage, the extraction plate 12 is in an inactive state, and the buckle 16 is in a locked state to ensure the condition of the items placed in the platform inside the box.

[0040] When storing items individually, simply open the latch 16 and lift the drawer 12 to operate. The drawer 12 is engaged in the main body slot 15 and can slide up and down along the slot 15, preventing the drawer 12 from touching the items inside the box when being pulled out or lowered, thus avoiding unnecessary accidents. When the movable top panel 13 is opened, the storage box 2 can be inserted into the integrated drawer box 1 through this opening.

[0041] Before liquid nitrogen fumigation, insert the item information card into the information card placement area 22, then place the item to be fumigated into the loading cavity 21, and then insert the loading box slot 23 into the card support wall 31 of the constant distance fumigation card holder 3, ensuring that the loading cavity 21 is completely embedded in the hollow structure, and then the liquid nitrogen fumigation procedure can be carried out.

[0042] During liquid nitrogen fumigation, the fixing ring 33 prevents the constant-distance fumigation holder 3 from moving excessively due to the liquid nitrogen, which could cause the loading cavity 21 to vibrate and affect the fumigation results. After fumigation, lift the integrated extraction box 1, open the latch 16, and lift the extraction plate 12 upwards. Then, detach the loading box 2 from the constant-distance fumigation holder 3, and slightly lift the opening of the loading cavity 21 to prevent items from falling. Place the detached loading box 2 into the integrated extraction box 1 with the information card placement area 22 facing the extraction plate 12. After placing it stably, lower the extraction plate 12 and close the latch 16 to complete the fumigation and storage operation.

[0043] The above are merely specific embodiments of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions within the technical scope disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims.

Claims

1. A constant-distance liquid nitrogen fumigation and storage integrated device, characterized in that, The device includes an integrated extraction box, a storage box, and a constant-distance fumigation holder. The integrated extraction box comprises a main body, an extraction plate, a movable top plate, and a handle. The main body is a cuboid structure with openings on both the front and top surfaces. The extraction plate is slidably connected to the opening on the front surface of the main body. The movable top plate is detachably connected to the opening on the top surface of the main body. The lower end of the handle is connected to the main body, and the upper end forms a hook. The constant-distance fumigation holder is hung on the handle. The interior of the constant-distance fumigation holder forms an installation cavity. The storage box is located within the installation cavity. The storage box is a cuboid structure with a perforated front surface. The storage box has storage box slots on two opposite sides.

2. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The main housing includes a base plate and a left side plate, a right side plate, and a rear side plate fixed to the base plate; the inner sides of the left side plate and the right side plate are provided with main housing slots; the pull-out plate can slide up and down in the main housing slots.

3. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The movable top plate and the extraction plate are connected by a snap fastener.

4. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The hook section at the upper end of the handle includes a horizontally bent section and a vertically bent section.

5. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The front surface of the container is provided with an information card placement area.

6. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 5, characterized in that, The information card placement area is a panel welded to the front surface of the container; the panel has an installation slot for placing the information card.

7. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, There are multiple containers; The upper surface of the container has two container clips that are adapted to the container slot.

8. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The side of the constant-distance fumigation holder is provided with a fixing ring.

9. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The dimensions of the container are compatible with the mounting cavity of the constant-distance fumigation card holder and the inner cavity of the integrated extraction box.

10. The integrated device for constant-distance liquid nitrogen fumigation and storage according to claim 1, characterized in that, The container is made of refrigerant-coated sheet.

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