Efficient stem cell sampling equipment

Abstract

The utility model relates to the technical field of stem cell culture, and discloses efficient stem cell sampling equipment which comprises a sampling box, the top of an assembly cavity is fixedly connected with a mounting plate, a sampling barrel is embedded in the center of the mounting plate, a rotating mechanism is arranged in the center of the sampling barrel, and the rotating mechanism is connected with the mounting plate. The top of the rotating mechanism is connected with a containing mechanism, the containing mechanism comprises a containing disc, clamping grooves, a first sampling assembly, a mounting groove and a second sampling assembly, the multiple clamping grooves are formed in the circumference of the edge of the containing disc at equal intervals, the first sampling assembly is connected into the clamping grooves in a clamped mode, and the second sampling assembly is connected into the mounting groove in a clamped mode. According to the equipment, the accommodating mechanism is arranged, the sampling assembly I and the sampling assembly II are used for stably placing the sampling tube and the sampling box on the accommodating disc, and the accommodating disc is driven by the rotating mechanism to rotate so as to realize sampling, so that the adaptability of the equipment to different sampling containers is improved, and the equipment is convenient to operate and replace parts.

Description

TECHNICAL FIELD

[0001] The utility model relates to stem cell culture technical field, concretely is a kind of efficient stem cell sampling equipment. BACKGROUND

[0002] Cells are a class of special cells with self-renewal ability and differentiation potential. They can split to produce more stem cells in vivo or in vitro, or differentiate into various types of mature cells. Stem cell research plays an important role in the fields of regenerative medicine, disease treatment and tissue engineering. Stem cell sampling technology is the basis of this research field, which directly affects the quality of cells and the success rate of subsequent experiments.

[0003] For example, Chinese patent CN202021476050.4 discloses a sampling device for stem cells. First, the sampling tube for stem cells can be centrifuged. The sampling tube for stem cells can also be inverted to ensure that the stem cells can be quickly and completely precipitated, thereby improving the sampling efficiency and ensuring the stability of the sampling tube for stem cells during centrifugation and inversion.

[0004] However, this device is only suitable for stem cell sampling with specific round tube-shaped sampling tubes. Since square culture boxes are also widely used in the cultivation process, this limits the scope of use of the device. In addition, the operation process is complex, which is not conducive to large-scale application, resulting in instability of the sampling effect, and thus affecting the quality of cells and the success rate of experiments.

[0005] Therefore, the utility model designs an efficient stem cell sampling device to solve the above problems. UTILITY MODEL CONTENT

[0006] The utility model aims to provide an efficient stem cell sampling device to solve the problems raised in the background art.

[0007] To solve the above technical problems, the utility model provides the following technical scheme: an efficient stem cell sampling device, comprising a sampling box, a mounting cavity is formed in the inside of the sampling box, a mounting plate is fixedly connected to the top of the mounting cavity, a sampling cylinder is embedded at the center position of the mounting plate, a rotating mechanism is arranged at the center position of the sampling cylinder, a containing mechanism is connected to the top of the rotating mechanism, the containing mechanism comprises a containing disc, a clamping groove, a sampling assembly one, a mounting groove and a sampling assembly two, a plurality of clamping grooves are circumferentially and equally spaced apart at the edge of the containing disc, the sampling assembly one is clamped and connected in the clamping groove, the mounting groove is arranged at the shaft center close to the containing disc of the clamping groove, the mounting groove is formed in the containing disc, and the sampling assembly two is clamped and connected in the mounting groove.

[0008] Preferably, the sampling assembly one includes a connecting plate, a fixed plate and a sampling tube, the connecting plate is clamped and connected in the clamping groove, the top of the connecting plate is fixedly connected with the fixed plate, the fixed plate is vertically arranged with the connecting plate, and the fixed plate is embedded with the detachably connected sampling tube.

[0009] Preferably, the sampling assembly two includes a limiting frame, a fixed cylinder, a placing plate, a limiting groove and a sampling box, the limiting frame is clamped and connected in the mounting groove, the side wall of the limiting frame is fixedly connected with the fixed cylinder, the end, away from the limiting frame, of the fixed cylinder is fixedly connected with the placing plate, the placing plate is provided with the limiting groove, and the limiting groove is embedded with the sampling box.

[0010] Preferably, the fixed cylinder is fixedly connected with a limiting spring, one end of the limiting spring, close to the placing plate, is fixedly connected with a limiting plate, and the limiting plate is abutted with the placing plate.

[0011] Preferably, the left and right sides of the placing plate are fixedly connected with limiting rods, and the limiting rods are clamped and connected in the grooves correspondingly arranged on the top of the containing disc.

[0012] Preferably, the rotating mechanism includes a base, a rotating motor and a rotating shaft, the base is fixedly connected at the bottom of the assembly cavity, the rotating motor is fixedly connected at the center position of the top of the base, the output end of the rotating motor is connected with the rotating shaft, and the top end of the rotating shaft is fixedly connected with the shaft center of the containing disc.

[0013] Compared with the prior art, the utility model has the advantages that:

[0014] The utility model discloses a sampling device, which comprises a containing mechanism, a rotating mechanism, a sampling assembly one and a sampling assembly two, the containing mechanism is arranged on the bottom of the assembly cavity, the sampling assembly one is arranged on the top of the containing mechanism, the sampling assembly two is arranged on the top of the containing mechanism, and the rotating mechanism is arranged on the top of the containing mechanism. BRIEF DESCRIPTION OF DRAWINGS

[0015] Figure 1 It is the whole structure schematic diagram of the utility model;

[0016] Figure 2 It is the main view cross section structure schematic diagram of the utility model.

[0017] Figure 3 It is structure schematic view of sampling assembly one in the utility model;

[0018] Figure 4 It is structure schematic view of sampling assembly two in the utility model;

[0019] Figure 5 It is cross section schematic view of sampling assembly two in the utility model;

[0020] Figure 6 It is structure schematic view of rotating mechanism in the utility model.

[0021] Among them: 1, sampling box;2, assembly cavity;3, mounting plate;4, sampling cylinder;5, rotating mechanism;501, base;502, rotating motor;503, rotating shaft;6, containing mechanism;601, containing disc;602, clamping groove;603, sampling assembly one;6031, connecting plate;6032, fixed plate;6033, sampling pipe;604, mounting groove;605, sampling assembly two;6051, limiting frame;6052, fixed cylinder;6053, placing plate;6054, limiting groove;6055, sampling box;7, limiting spring;8, limiting plate;9, limiting rod. DETAILED DESCRIPTION

[0022] The technical solutions in the embodiments of the utility model will be clearly and completely described below with reference to the drawings in the embodiments of the utility model. Obviously, the described embodiments are only part of the embodiments of the utility model, rather than all the embodiments. Based on the embodiments in the utility model, all other embodiments obtained by those skilled in the art without creative labor fall within the protection scope of the utility model. Embodiment 1

[0023] Please refer to Figure 1 - Figure 5 It is illustrated that one kind high-efficiency stem cell sampling equipment in the description embodiment 1, sampling box 1 is included, the inside of sampling box 1 is opened and is equipped with assembly cavity 2, the top of assembly cavity 2 is fixedly connected with mounting plate 3, the center position of mounting plate 3 is embedded with sampling cylinder 4, the center position of sampling cylinder 4 is provided with rotating mechanism 5, the top of rotating mechanism 5 is connected with containing mechanism 6, containing mechanism 6 includes containing disc 601, clamping groove 602, sampling assembly one 603, mounting groove 604 and sampling assembly two 605, the edge of containing disc 601 is circumferentially and equidistantly opened with several clamping grooves 602, clamping groove 602 is hingedly connected with sampling assembly one 603, clamping groove 602 is close to the shaft center of containing disc 601 and is provided with mounting groove 604, mounting groove 604 is opened on containing disc 601, and mounting groove 604 is hingedly connected with sampling assembly two 605.

[0024] Referring to Figure 3 and Figure 5 The sampling assembly one 603 in the drawing includes a connecting plate 6031, a fixed plate 6032 and a sampling tube 6033, the connecting plate 6031 is clamped and connected in the clamping groove 602, the top of the connecting plate 6031 is fixedly connected with the fixed plate 6032, the fixed plate 6032 is vertically arranged with the connecting plate 6031, and the sampling tube 6033 is detachably arranged in the fixed plate 6032.

[0025] Further, the sampling assembly two 605 includes a limiting frame 6051, a fixed cylinder 6052, a placing plate 6053, a limiting groove 6054 and a sampling box 6055, the limiting frame 6051 is clamped and connected in the mounting groove 604, the sidewall of the limiting frame 6051 is fixedly connected with the fixed cylinder 6052, one end of the fixed cylinder 6052 away from the limiting frame 6051 is fixedly connected with the placing plate 6053, the limiting groove 6054 is arranged in the placing plate 6053, and the sampling box 6055 is arranged in the limiting groove 6054.

[0026] In the embodiment, when the stem cells cultured in the sampling tube 6033 and the sampling box 6055 are sampled, the sampling tube 6033 is inserted on the fixed plate 6032, and is fixed on the containing disc 601 through the connecting plate 6031, the sampling box 6055 is driven to rotate the containing disc 601 through the rotating mechanism 5, so that the sampling assembly one 603 and the sampling assembly two 605 rotate around the axial center in the sampling cylinder 4, in the rotating process, the stem cell culture solution in the sampling tube 6033 and the sampling box 6055 is pushed to the end of the sampling tube 6033 and the sampling box 6055 through the action of the centrifugal force, after the sampling is completed, the material is taken out, and the next set of sampling assembly one 603 and sampling assembly two 605 are installed to carry out the next sampling, the whole process can be continuously carried out, through the efficient stem cell sampling equipment, the rapid, continuous and accurate stem cell sampling can be realized, the sampling efficiency and the survival rate of the stem cells are greatly improved, the maintenance and replacement of the parts are simple and convenient, and the use efficiency and the reliability of the equipment are further improved. Specifically, the sampling assembly one 603 and the sampling assembly two 605 arranged on the containing mechanism 6 can place a plurality of sets of sampling tubes 6033 and sampling boxes 6055 on the containing disc 601 to carry out the sampling operation, the adaptability of the sampling equipment to the sampling container is improved, sampling operation on different specifications and types of sampling containers is facilitated, in addition, the structural design of the sampling assembly one 603 and the sampling assembly two 605 makes the sampling process more stable and safe, and the falling or damage of the sampling tube 6033 and the sampling box 6055 in the high-speed rotating process is avoided. Example 2

[0027] Referring to Figure 1 and Figure 6As shown in the schematic diagram, the fixed cylinder 6052 is fixedly connected with a limiting spring 7, the limiting spring 7 is fixedly connected with a limiting plate 8 close to one end of the placement plate 6053, and the limiting plate 8 abuts against the placement plate 6053.

[0028] Further, the left and right sides of the placement plate 6053 are fixedly connected with limiting rods 9 which are clamped in the corresponding grooves on the top of the containing disc 601.

[0029] In this embodiment, when the sampling box 6055 is inserted into the limiting groove 6054, the rear side wall of the sampling box 6055 abuts against the limiting plate 8 under the action of the limiting spring 7, so that the sampling box 6055 can be stably placed in the placement plate 6053. Meanwhile, the limiting rods 9 arranged on the left and right sides of the placement plate 6053 are embedded in the corresponding grooves on the containing disc 601 for limiting, so that the sampling box 6055 can be kept stable when rotating under the action of the rotating mechanism 5, avoiding displacement or falling due to centrifugal force. The double limiting design of the limiting spring 7 and the limiting rod 9 ensures the safety of the sampling box 6055 during high-speed rotation, thereby improving the stability and reliability of the sampling process. Embodiment 3

[0030] As shown in the schematic diagram, Figure 2 and Figure 6 As shown in the schematic diagram, the rotating mechanism 5 includes a base 501, a rotating motor 502 and a rotating shaft 503. The base 501 is fixedly connected to the bottom of the assembly cavity 2. The rotating motor 502 is fixedly connected to the center of the top of the base 501. The output end of the rotating motor 502 is connected with the rotating shaft 503, and the top end of the rotating shaft 503 is fixedly connected with the shaft center of the containing disc 601. In this embodiment, when the sampling assembly one 603 and the sampling assembly two 605 in the containing mechanism 6 are assembled on the containing disc 601, the rotating mechanism 5 starts to work. The base 501 provides stable support, and the rotating motor 502 drives the containing disc 601 to rotate at high speed through the rotating shaft 503. The rotating speed of the rotating motor 502 can be accurately adjusted through the control circuit to adapt to different types of stem cell sampling requirements. In order to ensure the stability during rotation, the rotating shaft 503 is supported by high-precision bearings to reduce friction and vibration, thereby ensuring the accuracy and repeatability of the sampling process.

[0031] It should be noted that the front side of the sampling box 1 is also provided with a control unit connected with the rotating mechanism 5, which is used to control the start, stop and rotating speed of the rotating mechanism 5, through the control unit, the user can set the rotating speed and rotating time to adapt to the sampling requirements of different types of stem cell culture solution, for example, for the culture solution with higher viscosity, a lower rotating speed and a longer rotating time can be set to ensure the smooth progress of the sampling process.

[0032] Although the embodiments of the present application have been shown and described, it should be understood by those skilled in the art that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present application, and the scope of the present application is defined by the appended claims and their equivalents.

Claims

1. A high efficiency stem cell sampling device comprising a sampling box (1) characterized in that: The inside of the sampling box (1) is provided with an assembly cavity (2), the top of the assembly cavity (2) is fixedly connected with a mounting plate (3), the center position of the mounting plate (3) is embedded with a sampling cylinder (4), the center position of the sampling cylinder (4) is provided with a rotating mechanism (5), the top of the rotating mechanism (5) is connected with a containing mechanism (6), the containing mechanism (6) includes a containing disc (601), a clamping groove (602), a sampling assembly one (603), a mounting groove (604) and a sampling assembly two (605), a plurality of clamping grooves (602) are circumferentially and equidistantly provided at the edge of the containing disc (601), the clamping groove (602) is hingedly connected with the sampling assembly one (603), the clamping groove (602) is provided with a mounting groove (604) near the shaft center of the containing disc (601), the mounting groove (604) is provided on the containing disc (601), and the mounting groove (604) is hingedly connected with the sampling assembly two (605).

2. The efficient stem cell sampling device of claim 1, wherein: The sampling assembly one (603) includes a connecting plate (6031), a fixed plate (6032) and a sampling pipe (6033), the connecting plate (6031) is hingedly connected in the clamping groove (602), the top of the connecting plate (6031) is fixedly connected with the fixed plate (6032), the fixed plate (6032) is vertically arranged with the connecting plate (6031), and the fixed plate (6032) is embedded with a detachably connected sampling pipe (6033).

3. The efficient stem cell sampling device of claim 1, wherein: The sampling assembly two (605) includes a limiting frame (6051), a fixed cylinder (6052), a placing plate (6053), a limiting groove (6054) and a sampling box (6055), the limiting frame (6051) is hingedly connected in the mounting groove (604), the side wall of the limiting frame (6051) is fixedly connected with the fixed cylinder (6052), one end of the fixed cylinder (6052) away from the limiting frame (6051) is fixedly connected with the placing plate (6053), the limiting groove (6054) is provided in the placing plate (6053), and the limiting groove (6054) is embedded with the sampling box (6055).

4. The efficient stem cell sampling device of claim 3, wherein: The limiting spring (7) is fixedly connected in the fixed cylinder (6052), one end of the limiting spring (7) close to the placing plate (6053) is fixedly connected with a limiting plate (8), and the limiting plate (8) abuts against the placing plate (6053).

5. The efficient stem cell sampling device of claim 4, wherein: The limiting rods (9) are fixedly connected on the left and right sides of the placing plate (6053), and the limiting rods (9) are hingedly connected in the grooves correspondingly provided on the top of the containing disc (601).

6. The efficient stem cell sampling device of claim 1, wherein: The rotating mechanism (5) includes a base (501), a rotating motor (502) and a rotating shaft (503), the base (501) is fixedly connected at the bottom of the assembly cavity (2), the center position of the top of the base (501) is fixedly connected with the rotating motor (502), the output end of the top of the rotating motor (502) is connected with the rotating shaft (503), and the top end of the rotating shaft (503) is fixedly connected with the shaft center of the containing disc (601).

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