Environment-friendly row type pigment chromatography device

Abstract

The utility model provides an environment-friendly row type pigment chromatography device which comprises chromatography tubes, a flow divider and an injector, the chromatography tubes are used for loading a stationary phase, the bottoms of the multiple chromatography tubes are communicated with the flow divider so that the multiple chromatography tubes can be connected to the flow divider in parallel, the injector is used for loading chromatography liquid, and the flow divider is communicated with the flow divider so that the multiple chromatography tubes can be connected to the flow divider in parallel. And the output end of the injector is communicated with the flow divider. According to the invention, multiple groups of pigment chromatography experiments can be completed at one time through the multiple parallel chromatography tubes, and multiple samples can be treated at the same time, so that time and resources are saved, and the experiment efficiency is improved; meanwhile, the environment-friendly row type pigment chromatography device has good air tightness, leakage of poisonous gas in the experiment process can be avoided, after the experiment is finished, chromatography liquid can be recycled through an injector and can be reused, waste liquid discharge is reduced, pollution to the environment is reduced, and the purposes of energy conservation and environment protection are achieved.

Description

TECHNICAL FIELD

[0001] The application relates to the technical field of teaching aids, in particular to an environment-friendly row type pigment chromatography device. BACKGROUND

[0002] The photosynthetic pigment separation experiment is an important content of biological experiment teaching, and the main principle is that different types of pigments have different solubilities in the chromatography liquid, so that the diffusion speeds of the pigments on the qualitative filter paper are different, and the separation of the pigments is realized by using the characteristics. At present, the chromatography device used for pigment separation is relatively simple, and a test tube and a cotton plug or a small beaker and a culture dish cover are used for assembly. During the experiment, the device is assembled first, then a small amount of chromatography liquid is added into the test tube or the small beaker, a filter paper strip containing a pigment filtrate thread is placed in the chromatography liquid, the end of the filter paper strip is soaked in the chromatography liquid, and the chromatography separation of the pigments can be performed after the cotton plug is tightly plugged or the culture dish cover is covered.

[0003] However, the traditional chromatography device used at present is composed of a small beaker, a culture dish cover, a test tube and a cotton plug, and has poor sealing performance. During the experimental operation process, whether installation, use or disassembly after the end of the experiment, the leakage of the toxic chromatography liquid cannot be effectively prevented. In addition, since the chromatography liquid is not used quantitatively, too much chromatography liquid is often used during the operation process, or the position of the pigment filtrate thread on the filter paper is too low, so that the chromatography liquid is higher than the filtrate thread, the chromatography separation of the pigments fails, and the chromatography liquid is contaminated, thereby causing the waste of reagents. CONTENT OF THE INVENTION

[0004] The purpose of the application is to provide an environment-friendly row type pigment chromatography device capable of realizing quantitative and uniform matching of chromatography liquid, and having the advantages of high experimental efficiency.

[0005] In order to achieve the above purpose, the application provides the following technical scheme:

[0006] An environment-friendly row type pigment chromatography device, which comprises a chromatography tube, a flow divider and a syringe, the chromatography tube is used for loading a stationary phase, the chromatography tube is provided with multiple branches, and the bottoms of the multiple chromatography tubes are respectively communicated with the flow divider so that the multiple chromatography tubes are connected in parallel on the flow divider, and the syringe is used for loading a chromatography liquid, and the output end of the syringe is communicated with the flow divider.

[0007] Further provided is that the top of the chromatography tube is provided with a tube opening, and the bottom of the chromatography tube is provided with a narrow neck opening, and the bottom of the chromatography tube is connected with the flow divider through the narrow neck opening.

[0008] Further provided is that the chromatography tube is provided with a sealing plug at the tube opening of the chromatography tube for sealing the tube opening.

[0009] Further arrangement: the flow divider comprises a flow pipe and branch pipes arranged on the flow pipe, the branch pipes are used to connect with the narrow necks of the chromatography tubes, the chromatography tubes are arranged along the extending direction of the flow pipe, and the chromatography tubes are connected with the branch pipes one by one.

[0010] Further arrangement: two narrow neck branch pipes are arranged below the chromatography tube, and the two adjacent chromatography tubes are connected through the narrow neck branch pipes.

[0011] Further arrangement: the chromatography tube comprises two first test tubes, one of the two narrow neck branch pipes of the first test tube is used to connect with the adjacent chromatography tube, and the other narrow neck branch pipe is used as a free narrow neck branch pipe.

[0012] The free narrow neck branch pipe of one of the first test tubes is arranged in a sealed manner, and the free narrow neck branch pipe of the other first test tube is connected with an air bag.

[0013] Further arrangement: a second test tube is arranged between the two first test tubes, the second test tube is arranged at least once, and the two narrow neck branch pipes of the second test tube are used to connect with the adjacent chromatography tubes.

[0014] Further arrangement: the flow divider further comprises a connecting branch pipe arranged on the flow pipe, the connecting branch pipe is used to connect with the output end of the syringe, and a flow adjusting structure is arranged on the connecting branch pipe.

[0015] Further arrangement: the syringe comprises a barrel and a piston push rod, one end of the barrel is provided with a connector used to connect with the connecting branch pipe of the flow divider, and the piston push rod reciprocates towards or away from the connector.

[0016] Further arrangement: scales are arranged on the chromatography tube and the syringe.

[0017] Compared with the prior art, the scheme of the present application has the following advantages:

[0018] 1. The environmental protection type row type pigment chromatography device disclosed by the present application can complete a plurality of groups of pigment chromatography experiments at one time through the plurality of chromatography tubes in parallel, can process a plurality of samples at the same time, thereby saving time and resources and improving experimental efficiency; meanwhile, the environmental protection type row type pigment chromatography device has good air tightness, and the structures such as the sealing plug, the water stop clamp and the air bag can ensure that there is no toxic gas leakage in the whole experiment process, improve the experimental safety of the experimenter, avoid pollution to the environment, the air bag can also recycle toxic volatile gas, and after the experiment is completed, the chromatography liquid can be recycled by using the syringe and can be reused, waste liquid discharge is reduced, pollution to the environment is reduced, and the purpose of energy saving and environmental protection is achieved.

[0019] 2. In the environmental protection type of the present application, the scale on the chromatography tube can realize non-contact measurement of the position of each sample pigment band, and can simultaneously compare and analyze multiple experimental results, which is beneficial to enhance the experimental teaching effect.

[0020] 3. In the environmental protection type of the present application, the chromatography liquid can be slowly injected into the chromatography tube through the syringe and the plug, so as to ensure that the chromatography tube does not pass through the filter paper fine line, and ensure the smooth progress of the pigment chromatography experiment.

[0021] The additional aspects and advantages of the present application will be partially given in the following description, which will become apparent from the following description, or will be understood by those skilled in the art through the practice of the present application. BRIEF DESCRIPTION OF DRAWINGS

[0022] The above and / or additional aspects and advantages of the present application will become apparent and more readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

[0023] Figure 1 It is a front view of an embodiment of the environmental protection type of the present application;

[0024] Figure 2 It is a perspective view of an embodiment of the environmental protection type of the present application;

[0025] Figure 3 It is an enlarged schematic view of A part of Figure 2

[0026] In the figure, 1 is a chromatography tube; 11 is a narrow neck; 12 is a narrow neck branch; 13 is a water stop clamp; 14 is an air bag; 15 is a sealing plug; 101 is a first test tube; 102 is a second test tube; 2 is a flow divider; 21 is a flow dividing pipe; 22 is a branch pipe; 23 is a connecting branch pipe; 24 is a plug; 3 is a syringe; 31 is an empty barrel; 32 is a piston push rod; 33 is a joint; 4 is a hose; 5 is a scale. DETAILED DESCRIPTION

[0027] The embodiments of the present application will be described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference signs represent the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be interpreted as a limitation on the present application.

[0028] It should be understood that each step recorded in the method embodiment of the present application can be executed in different order and / or in parallel. In addition, the method embodiment can include additional steps and / or omit the execution of the shown steps. The scope of the present application is not limited in this respect.

[0029] ​The term "includes" and its variants are meant to cover a non-exclusive inclusion, i.e. "including, but not limited to". The term "connected" can be direct or indirect, through intermediary parts (elements). The term "one embodiment" means "at least one embodiment"; the term "another embodiment" means "at least one additional embodiment"; the term "some embodiments" means "at least some embodiments". Related definitions are given throughout the description.

[0030] It should be noted that the terms "first", "second", and the like in the present utility model are only used to distinguish the devices, modules or units, and are not intended to limit the devices, modules or units to be different devices, modules or units, nor to limit the order or interdependence of the functions performed by the devices, modules or units.

[0031] In view of the problem that the traditional chromatography device cannot conveniently realize quantitative use of chromatography liquid, the present application provides an environmentally friendly row type pigment chromatography device (hereinafter referred to as "chromatography device") which, in combination with Figures 1 to 3 It comprises a chromatography tube 1, a flow divider 2 and a syringe 3, wherein the chromatography tube 1 is used to load a stationary phase, the chromatography tube 1 is provided with multiple branches, the bottoms of the multiple chromatography tubes 1 are communicated with the flow divider 2 so that the multiple chromatography tubes 1 are connected in parallel on the flow divider to realize the row structure of the multiple chromatography tubes 1, the syringe 3 is used to load chromatography liquid, and the output end of the syringe 3 is connected with the flow divider 2, so that the chromatography liquid can be input into the flow divider 2 through the syringe 3, and then the chromatography liquid is uniformly distributed into each chromatography tube 1 by the flow divider 2, thereby achieving the purpose of quantitative distribution and use of the chromatography liquid, avoiding errors caused by manual distribution, reducing manual operation steps, and improving experimental efficiency.

[0032] Specifically, the top of the chromatography tube 1 is a tube opening, and the bottom thereof is provided with a narrow neck opening 11, and the chromatography tube 1 is connected with the flow divider 2 through the narrow neck opening 11 at the bottom thereof to realize the communication between the chromatography tube 1 and the flow divider 2. The chromatography tube 1 of the present embodiment adopts a cylindrical glass tube, the height of which is 100 mm, and the outer diameter of which is 15 mm.

[0033] Further, the chromatography tube 1 is provided with two narrow neck branch pipes 12 at a set height position below the tube opening thereof, and adjacent two chromatography tubes 1 can be connected with the narrow neck branch pipes 12 through a hose 4, thereby realizing the parallel connection between the multiple chromatography tubes 1. The distance between the narrow neck branch pipes 12 on the chromatography tube 1 and the tube opening thereof is 30 mm, and the two narrow neck branch pipes 12 are respectively located at opposite sides of the chromatography tube 1.

[0034] The shunt 2 of the present application comprises a shunt pipe 21 and branch pipes 22 arranged on the shunt pipe 21, and the branch pipes 22 are arranged in the extending direction of the shunt pipe 21. The branch pipes 22 are used to be connected with the narrow necks 11 at the bottom of the chromatography tubes 1, and the connection can be achieved by the flexible pipes 4 which are sleeved on the branch pipes 22 and the narrow necks 11 respectively. In the embodiment, the plurality of chromatography tubes 1 are arranged in the extending direction of the shunt pipe 21, and the branch pipes 22 on the shunt pipe 21 are connected with the chromatography tubes 1 one by one. In order to facilitate the connection of the branch pipes 22 with the narrow necks 11 of the chromatography tubes 1, the branch pipes 22 can be arranged upward to facilitate the alignment and connection of the branch pipes 22 with the narrow necks 11 of the chromatography tubes 1, reduce the operation difficulty of the connection, and ensure the tightness of the connection and reduce the possibility of leakage.

[0035] Through the above-mentioned connection of the plurality of chromatography tubes 1 on the shunt pipe 21 through the branch pipes 22 and the connection of the adjacent two chromatography tubes 1 through the narrow neck branch pipes 12, the side-by-side structure of the chromatography tubes 1 is formed, so that the purpose of consistent amount of chromatography liquid entering into each chromatography tube 1 through the shunt 2 can be achieved according to the principle of communicating vessels.

[0036] In an embodiment, the chromatography tube 1 comprises two first test tubes 101, and one of the two narrow neck branch pipes 12 of the two first test tubes 101 is used to be connected with the adjacent chromatography tube 1, and the other narrow neck branch pipe 12 is used as a free narrow neck branch pipe. Since the chromatography liquid in the chromatography experiment may be volatile, the free narrow neck branch pipe of one of the two first test tubes 101 is sealed, and the free narrow neck branch pipe of the other first test tube 101 is connected with an air bag 14. Specifically, the free narrow neck branch pipe which is sealed can be sleeved with a flexible pipe 4, and a water stop clamp 13 is arranged on the flexible pipe 4 to achieve the effect of sealing the narrow neck branch pipe 12. The air bag 14 is used to collect the volatile substances which are toxic or carcinogenic in the chromatography separation process, so as to reduce the pollution to the environment. The air bag 14 can also be used to buffer the system pressure of the chromatography device. When the internal pressure of the chromatography device increases, the balloon will expand to absorb the excess pressure; when the pressure decreases, the balloon will contract to release the stored pressure, so as to prevent the adverse effects on the chromatography device or the experiment caused by the sudden change of pressure.

[0037] When the number of chromatography tubes 1 is greater than 2, the chromatography tube 1 further comprises a second test tube 102 arranged between the two first test tubes 101, and the two narrow neck branch pipes 12 of the second test tube 102 are used to be connected with the narrow neck branch pipes 12 of the adjacent chromatography tubes 1, so as to connect the plurality of chromatography tubes 1 in parallel.

[0038] The plurality of chromatography tubes 1 can be split into single tubes for convenient transportation, and different numbers of chromatography tubes 1 can be installed according to actual experimental requirements. In order to further improve the experimental efficiency, the plurality of chromatography tubes 1 can also be processed into an integrated structure, that is, the adjacent two chromatography tubes 1 are connected by sharing the same neck branch pipe 12, thereby reducing the risk of leakage.

[0039] To this end, the chromatography tube 1 and the flow divider 2 can also adopt an integrated structure, that is, the neck port 11 of the chromatography tube 1 is fixedly connected with the branch pipe 22 of the flow divider 2, thereby reducing the steps of manual installation and reducing the risk of leakage of chromatography liquid.

[0040] The connection branch pipe 23 of the shunt pipe 21 is also provided for connecting with the output end of the syringe 3, and when the branch pipe 22 of the shunt pipe 21 is arranged upward, the connection branch pipe 23 is arranged downward. The connection branch pipe is also provided with a flow adjusting structure, and the flow adjusting structure of the embodiment adopts a plug 24 which can rotate relative to the connection branch pipe. The plug 24 can control the opening and closing of the connection branch pipe 23, and can also be used as a flow regulator to adjust the liquid flow of the connection branch pipe 23, so as to realize more precise control of the chromatography liquid.

[0041] The syringe 3 is arranged below the flow divider 2, and the syringe 3 includes a barrel 31 and a piston push rod 32. One end of the barrel 31 is provided with a connector 33 for connecting with the connection branch pipe, and the connector 33 serves as the output end of the syringe 3 for connecting with the connection branch pipe 23 of the shunt pipe 21. The piston push rod 32 is located in the barrel 31 and reciprocates towards or away from the connector 33, so as to input the chromatography liquid loaded in the barrel 31 into the shunt pipe 21, or to recover the chromatography liquid in the shunt pipe 21 into the barrel 31. Therefore, the experimenter can manually operate or combine with the injection pump to realize the control of the flow rate of the chromatography liquid, so as to further improve the capacity accuracy of the injection liquid input into the chromatography tube, and improve the accuracy of the experiment.

[0042] It should be noted that when the connector 33 of the syringe 3 is connected with the connection branch pipe 23, the air tightness of the connection should be paid attention to. In the embodiment, the connector 33 of the syringe 3 is directly connected with the connection branch pipe 23. In other embodiments, the connector 33 of the syringe 3 and the connection branch pipe 23 can also be connected through a hose, which can improve the flexibility of the connection between the syringe 3 and the connection branch pipe 23, and can also buffer the injection pressure fluctuation, and can match different specifications of the syringe 3 and the connection branch pipe 23.

[0043] In addition, in order to further improve the experimental accuracy, the present application is provided with the scale 5 along the height direction on the wall of the chromatography tube 1 and the outer wall of the empty barrel 31 of the syringe 3, the scale 5 has a range of 0-10 cm, and the scale is divided by 1 mm. The scale 5 can help the experimenter to observe the volume of the chromatography liquid entering the chromatography tube 1, and also can help the experimenter to observe and record the separation of the pigment, so as to more accurately understand the chromatography process. When the chromatography experiment is carried out by using the chromatography device of the present application, one of the chromatography tubes 1 is selected as a standard sample, and whether the chromatography experiment is finished is judged by observing the separation of the standard sample. The experimenter also needs to observe and record in time during the pigment separation process, and take pictures in time, so as to facilitate the analysis of the experimental results.

[0044] In order to ensure the overall airtightness of the chromatography experiment, the sealing plug 15 for sealing the mouth of the chromatography tube 1 is further provided at the mouth of each chromatography tube 1. The sealing plug 15 of the present embodiment can be a rubber plug or a silica gel plug. The mouth of the chromatography tube 1 can be sealed by the sealing plug 15, so as to ensure the airtightness of the chromatography device, avoid the harmful gas volatilized from the chromatography liquid from diffusing to the outside through the mouth to affect the health of the experimenter and the environment, and also avoid the experimental error caused by the volatilization of the chromatography liquid.

[0045] When the pigment chromatography experiment is carried out by using the environment-friendly row type pigment chromatography device of the present application, the environment-friendly row type pigment chromatography device is first fixed on the experimental support to ensure the stability of the experimental process, and also can facilitate the display of the experimental process and the experimental results. Five chromatography tubes 1 are provided in the present embodiment, and each of the chromatography tubes 1 is loaded with a stationary phase. Filter paper is used as the stationary phase in the present embodiment, and different samples are provided on the filter papers in different chromatography tubes 1 for chromatography separation.

[0046] The two thin neck branches 12 of the adjacent two chromatography tubes 1 are connected by the hose 4 to make the five chromatography tubes 1 in parallel, and the water stop clamp 13 and the air bag 14 are respectively installed on the free thin neck branches of the two first test tubes on the two sides. At the same time, the thin neck mouth 11 at the bottom of the chromatography tube 1 is also connected with the branch pipe 22 on the shunt pipe 21 through the hose 4, so as to realize the communication between the shunt pipe 21 and the chromatography tube 1.

[0047] The hose 4 is used to realize the connection between the pipes in the present application. Since the hose 4 has good plasticity, it can compensate for the positional deviation between the pipes, and ensure the airtightness of the connection between the pipes.

[0048] The syringe 3 is connected below the shunt 2, the joint 33 of the syringe 3 is connected with the connection branch pipe 23 of the shunt pipe 21. A 100ml syringe 3 is used in the present embodiment, and 50ml chromatography liquid is first sucked before being connected with the connection branch pipe 23, and the sealing property between the syringe 3 and the connection branch pipe 23 is also ensured.

[0049] After the environmental protection type row type pigment chromatography device is installed, the filter paper provided with different samples is placed into different chromatography tubes 1, the sealing plug 15 is tightly plugged, the stopcock 24 on the connecting branch pipe 23 at the bottom of the shunt pipe 21 is adjusted to adjust the flow, the piston push rod 32 of the syringe 3 is pushed to make the chromatography liquid in the empty barrel 31 input into the shunt pipe 21, and then the chromatography liquid is uniformly distributed into each chromatography tube 1 by the shunt pipe 21. During the process of pushing the piston push rod 32, the rising height of the chromatography liquid and the height of the filter paper fine line also need to be observed in real time. In the embodiment, the sample is coated on the filter paper fine line, and it is appropriate that the chromatography liquid does not submerge the filter paper fine line. After the height of the input chromatography liquid reaches the target height, the pigment chromatography separation is statically waited. Due to the principle of communicating vessels, the liquid level of the chromatography liquid entering each chromatography tube 1 through the shunt 2 is consistent, so that the equal distribution of the chromatography liquid can be realized.

[0050] The application judges whether the experiment is ended by observing the chromatography tube 1 provided with the standard sample. After the experiment is ended, the piston push rod 32 is pulled out towards the outside of the empty barrel 31 to recover the chromatography liquid in the chromatography tube 1 and the reflux pipe. After the chromatography liquid is completely recovered, the air bag 14 is tightly bound to recover the waste gas, the sealing plug 15 is opened to take out the filter paper, and the equipment can be arranged.

[0051] In summary, the environmental protection type row type pigment chromatography device can complete a plurality of pigment chromatography experiments at one time. Through the parallel connection mode, a plurality of samples can be processed at the same time, so that the time and resources are saved, and the experimental efficiency is improved. Meanwhile, the scale 5 arranged on the chromatography tube 1 can realize the non-contact measurement of the positions of the pigment bands of each sample, the comparison and analysis of the results of a plurality of experiments can be simultaneously performed, and the experimental teaching effect is enhanced.

[0052] The application can slowly inject the chromatography liquid into the chromatography tube 1 through the syringe 3 and the stopcock 24, so that the chromatography tube 1 does not submerge the filter paper fine line, and the smooth progress of the pigment chromatography experiment is ensured.

[0053] During the pigment chromatography experiment, the application can ensure that there is no toxic gas leakage in the whole experiment process, improve the experimental safety of the experimenter, and avoid pollution to the environment.

[0054] After the experiment is ended, the chromatography liquid can be recovered by the syringe 3 and can be reused, which is environmental protection and saving.

[0055] It should be noted that the specification and quantity of the chromatography tube 1, the shunt 2 and the syringe 3 are not limited, and the specification and quantity can be adaptively adjusted in the specific experiment.

[0056] It should be noted that the specification and quantity of the chromatography tube 1, the shunt 2 and the syringe 3 are not limited, and the specification and quantity can be adaptively adjusted in the specific experiment. The above only describes some embodiments of the application, and it should be noted that, for ordinary skilled persons in the technical field, some improvements and refinements can be made without departing from the principle of the application, and these improvements and refinements should also be regarded as the protection range of the application.

Claims

1. An environmentally friendly, multi-stage pigment chromatography device, characterized in that, The device includes a chromatography tube, a splitter, and a syringe. The chromatography tube is used to load a stationary phase. The chromatography tube has multiple sections, and the bottom of each section is connected to the splitter so that the multiple sections are connected in parallel on the splitter. The syringe is used to load a chromatography solution, and the output end of the syringe is connected to the splitter.

2. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 1, characterized in that, The chromatography tube has an opening at the top and a narrow neck at the bottom, and the bottom of the chromatography tube is connected to the splitter through its narrow neck.

3. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 2, characterized in that, The chromatography tube is equipped with a sealing plug at its opening for sealing the tube opening.

4. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 2, characterized in that, The splitter includes a split tube and a branch tube disposed on the split tube. The branch tube is used to connect to the narrow neck of the chromatography tube. The chromatography tubes are arranged along the path extension direction of the split tube, and the chromatography tubes are connected to the branch tubes in a one-to-one correspondence.

5. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 4, characterized in that, The chromatography tube has two narrow-necked branch tubes below its opening, and adjacent chromatography tubes are connected by the narrow-necked branch tubes.

6. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 5, characterized in that, The chromatography tube includes two first test tubes, one of which is used to connect adjacent chromatography tubes, and the other is used as a free narrow neck branch. One of the first test tubes has a sealed free-neck branch, while the other first test tube has a free-neck branch connected to an air bladder.

7. The environmentally friendly, multi-stage pigment chromatography apparatus according to claim 6, characterized in that, It also includes a second test tube disposed between the two first test tubes, wherein at least one second test tube is provided, and both narrow neck branches of the second test tube are used to connect to adjacent chromatography tubes.

8. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 4, characterized in that, The diverter also includes a connecting branch pipe disposed on the diverter tube, the connecting branch pipe being used to connect to the output end of the syringe, and the connecting branch pipe being provided with a flow adjustment structure.

9. The environmentally friendly multi-stage pigment chromatography apparatus according to claim 8, characterized in that, The syringe includes an empty cylinder and a piston rod. One end of the empty cylinder is provided with a connector for connecting to the connecting branch pipe of the distributor. The piston rod reciprocates in the direction of moving closer to or away from the connector.

10. The environmentally friendly multi-stage pigment chromatography apparatus according to any one of claims 1-9, characterized in that, Both the chromatography tube and the syringe are equipped with graduations.

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