Rapid urinary sediment filtering device

Abstract

The utility model relates to a rapid urinary sediment filtering device which comprises an extrusion structure and a filtering structure, urine is pushed by the extrusion structure to pass through the filtering structure, the filtering structure comprises a plurality of layers of ultra-thin filtering membranes, the aperture of each ultra-thin filtering membrane is 8-10 microns, and the thickness of each ultra-thin filtering membrane is 30-50 microns. The extrusion structure comprises a push rod, a piston and an extrusion cup, a through hole is formed in the lower end of the extrusion cup, and the through hole is connected with the filtering structure. According to the device, a multilayer grid structure with layer gaps formed among the multilayer ultra-thin filter membranes is fully utilized in a manner of overlapping and combining the multilayer ultra-thin filter membranes, so that sufficient urine can quickly pass through the filter membranes without blockage, urinary sediment is deposited on the filter membranes to the greatest extent, and the subsequent detection requirements are met. The device does not need additional equipment assistance, can be operated by a patient or family members at home, is suitable for more environments and places, and is simple in structure, small in size and convenient for mailing.

Description

Technical Field

[0001] This utility model relates to the field of medical testing equipment technology, specifically to a rapid urine sediment filtration device. Background Technology

[0002] With the development of sequencing technology, cancer screening based on next-generation sequencing (NGS) and its derivatives is becoming increasingly popular. High-throughput sequencing requires sufficient DNA samples. Currently, the sampling method for bladder cancer detection involves collecting first-morning urine and centrifuging it in a laboratory using a high-speed centrifuge to obtain urine sediment. The urine needs to be collected and centrifuged on-site, or transported via cold chain to a laboratory equipped with a high-speed centrifuge for processing. After obtaining the urine sediment, DNA is extracted for further testing. This collection method is overly complex and not convenient for patients to collect themselves, often requiring external equipment and cannot be completed at home.

[0003] Devices for obtaining urine sediment through filtration have emerged. For example, Chinese patent CN105722466B discloses a biofluid filtration component that uses a pressure device to assist filtration and can be used to obtain urine sediment. However, existing filtration methods for obtaining urine sediment do not collect a significantly larger amount of urine sediment compared to centrifugation, and the collection effect fluctuates greatly. The filter membrane is prone to clogging, and the collection speed is slow, thus lacking significant practical value in medical testing. Utility Model Content

[0004] To address the above problems, this utility model proposes a rapid urine sediment filtration device.

[0005] This invention provides a rapid urine sediment filtration device, comprising a squeezing structure and a filtration structure. The squeezing structure pushes urine through the filtration structure, which includes multiple ultra-thin filter membranes with a pore size of 8-10 μm and a thickness of 30-50 μm.

[0006] Furthermore, the extrusion structure includes a push rod, a piston, and an extrusion cup, with a through hole at the lower end of the extrusion cup, the through hole being connected to the filter structure.

[0007] Furthermore, the outer diameter of the piston is adapted to the inner diameter of the squeezing cup, the piston is provided with several annular grooves, and the push rod is provided with several annular protrusions below it. The annular grooves and annular protrusions are adapted to each other. After the piston is connected to the annular protrusions on the rod through the annular grooves, it is inserted into the squeezing cup to achieve sealed squeezing and push the urine to quickly pass through the filter structure connected to the through hole at the lower end of the squeezing cup.

[0008] Furthermore, the filter structure is detachably installed on the through hole. The filter structure is a lower cover with a through-hole structure and a groove on the inner wall. Two silicone gaskets are provided, and the multi-layer ultrathin filter membrane is provided between the two silicone gaskets.

[0009] Furthermore, the lower part of the push rod, piston, and extrusion cup is a layered, adaptable inverted truncated cone structure, and the through hole is located at the bottom of the lower inverted truncated cone of the extrusion cup.

[0010] Furthermore, the ultrathin filter membrane has three layers.

[0011] Furthermore, after the urine is pushed through the filtration structure by the extrusion structure, the waste liquid enters the waste liquid cup, which is either integrally prepared or separately prepared from the extrusion structure.

[0012] Furthermore, a mesh structure is provided on the through hole at the lower end of the extrusion cup.

[0013] Furthermore, a mesh structure is provided at the lower end of the lower cover.

[0014] The beneficial effects of this utility model are as follows:

[0015] 1. This device does not require any additional equipment and can be operated by the patient or their family at home. It is suitable for more environments and places, has a simple structure and small size, and is easy to mail.

[0016] 2. Existing filtration devices typically use single-layer, relatively thick membranes. The internal fibers often collapse upon wetting, obstructing flow. In practice, they are also directly clogged by proteins, bacteria, and polysaccharides in the urine, preventing proper collection of urine sediment. This device utilizes a multi-layered, ultra-thin membrane stacked together. The pore size of each ultra-thin membrane is 8-10 μm, and the thickness of each layer is 30-50 μm. This fully utilizes the multi-layered mesh structure with gaps between the membranes, ensuring sufficient urine flows quickly through without clogging. This maximizes urine sediment deposition on the membrane, meeting the requirements for subsequent testing. Using this device, urine sediment collection can be completed within 3 minutes. After the urine is expelled, the membrane containing urine sediment is placed in a preservation solution and sent back to the testing unit. Compared to existing filtration devices, this device increases the DNA extraction rate from the collected urine sediment by 5-10 times, significantly improving the accuracy and sensitivity of subsequent testing. Attached Figure Description

[0017] Figure 1 Schematic diagram of a rapid urine sediment filtration device.

[0018] Reference numerals: 1. Push rod; 2. Piston; 3. Squeezing cup; 4. Ultra-thin filter membrane; 5. Lower cover; 6. Silicone gasket. Detailed Implementation

[0019] The present invention will be further described below with reference to the embodiments.

[0020] Example 1: A rapid urine sediment filtration device:

[0021] like Figure 1 As shown, a rapid urine sediment filtration device includes a squeezing structure and a filtration structure. The squeezing structure pushes urine through the filtration structure, which includes multiple ultra-thin filter membranes 4. The pore size of the ultra-thin filter membranes 4 is 8-10 μm, and the thickness of the ultra-thin filter membranes 4 is 30-50 μm. The ultra-thin filter membranes 4 consist of 3 layers.

[0022] The extrusion structure includes a push rod 1, a piston 2, and an extrusion cup 3. The lower end of the extrusion cup 3 has a through hole, which is connected to the filter structure.

[0023] The outer diameter of the piston 2 is adapted to the inner diameter of the squeezing cup 3. The piston 2 is provided with several annular grooves, and the push rod 1 is provided with several annular protrusions below it. The annular grooves and annular protrusions are adapted to each other. After the piston 2 is connected to the annular protrusions on the rod 1 through the annular grooves, it is inserted into the squeezing cup 3 to achieve sealed squeezing and push the urine to quickly pass through the filter structure connected to the through hole at the lower end of the squeezing cup 3.

[0024] The filter structure is detachably installed on the through hole by a buckle. The filter structure is a lower cover 5. The lower cover 5 has a through structure with a groove on the inner wall and two silicone gaskets 6. The three layers of ultra-thin filter membrane 4 are arranged between the two silicone gaskets 6.

[0025] The lower parts of the push rod 1, piston 2, and squeeze cup 3 are layered, fitted inverted frustum structures, with the through hole located at the bottom of the inverted frustum of the squeeze cup 3. This inverted frustum structure accelerates the flow of urine into the filtration system.

[0026] Specifically, after the urine is pushed through the filter structure by the extrusion structure, the waste liquid enters the waste liquid cup, which is either integrally prepared or separately prepared from the extrusion structure.

[0027] Specifically, a mesh structure is provided on the through hole at the lower end of the squeeze cup 3. A mesh structure is also provided at the lower end of the lower cover 5. The mesh structure can prevent foreign objects from entering the filter structure.

[0028] When using:

[0029] Place the three - layer ultra - thin filter paper 4 between two silicone gaskets 6 of the lower cover 5. Then connect the lower cover 5 to the through - hole at the lower end of the extrusion cup 3 by snap - fitting, and the ultra - thin filter paper 4 is sealed between the extrusion cup 3 and the lower cover 5. Next, install the piston 2 to the lower part of the push rod 1. Then pour urine at the upper cup mouth above the extrusion cup, and then put the push rod 1 and the piston 2 into the extrusion cup 3. Place the device vertically in the waste liquid cup, and generate pressure by pushing the push rod 1 to squeeze the cup body, so as to push the urine through the ultra - thin filter paper 4. After extrusion, unfasten the snap - fitting, take out the ultra - thin filter paper 4 in the lower cover 5, place it in the corresponding preservation solution, and store it in a sealed bag for convenient preservation, transportation and subsequent DNA extraction.

[0030] Example 2: Comparison of the usage effects with existing filtering devices:

[0031] In this example, through comparative experiments, 20 cases of clinical pathological urine samples are used, and four groups are set up for effect comparison: conventional filter + conventional filtering material, conventional filter + the filtering material of this patent, the filter of this patent + conventional filtering material, and the filter of this patent + the filtering material of this patent. For each patient, 100 ml of urine is filtered in each group. The filtering duration is measured. The filter papers after filtration are treated with the same preservation method and DNA extraction method. Calculate the DNA extraction rate, and use an input amount of 10 ng, with beta - Actin as the internal reference gene, and detect the human DNA content by qPCR and calculate the average value.

[0032] Among them, the conventional filter used in this example is the connection of a conventional disposable needle - type filter head of a general medical syringe. After sucking urine with a disposable syringe, install the disposable needle - type filter head and perform filtration by manual pressurization. The conventional filter used in this example is the Cofoe brand disposable sterile medicine - dissolving syringe with a needle, Guix械注准20172140139. The connected disposable needle - type filter head is the Bicman Biotech (BKMAM) BKMAMLAB @ Needle - type filter, with a specification of 0.45μm.

[0033] The conventional filtering material used in this example is the filtering membrane integrated in the above - mentioned disposable needle - type filter head, with a thickness of 13 mm and a PES membrane with a pore size of 0.22μm - 0.45μm for the filtering membrane.

[0034] The filter of this patent is the extrusion structure and the lower cover described in Example 1;

[0035] The filtering material of this patent is the superposition combination of the three - layer ultra - thin filter membranes 4 described in Example 1.

[0036] The experimental results are shown in Table 1:

[0037] Table 1 Comparison of the usage effects between the filtering device of this patent and existing filtering devices

[0038]

[0039] As shown in Table 1, the use of the filter material of this patent, namely the three-layer ultra-thin filter paper, can significantly accelerate the urine filtration speed. If the filter and filter material of this patent are used simultaneously, the filtration time for 100 ml of urine is reduced from the conventional 33.4 minutes to 4.8 minutes, a significant reduction in filtration time. Furthermore, compared with conventional filter materials, the filter material of this patent can greatly improve the urine sediment collection rate, thereby increasing the DNA extraction yield by 3-4 times. The device and filter material of this patent not only have a fast filtration speed and a large amount of DNA extracted, but also a lower CT value with the same DNA input, proving that the content of human DNA is higher and the quality of the obtained human DNA is higher.

[0040] Therefore, this patented device can quickly separate urine samples from the test subject on-site within minutes, with a high urine sediment collection rate and a large amount of DNA extracted subsequently, without affecting further test results, and also avoids the problem of DNA decomposition and contamination caused by directly mailing urine.

[0041] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention.

[0042] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A rapid urine sediment filter device comprising a squeezing structure and a filtering structure, the urine is pushed through the filtering structure by the squeezing structure, characterized in that, The filter structure comprises a plurality of layers of ultra-thin filter membranes (4), the pore size of the ultra-thin filter membranes (4) is 8-10 μm, and the thickness of the ultra-thin filter membranes (4) is 30-50 μm.

2. The rapid urine sediment filtering device according to claim 1, wherein, The extrusion structure comprises a push rod (1), a piston (2), and an extrusion cup (3), the lower end of the extrusion cup (3) has a through hole, and the through hole is connected to the filter structure.

3. The rapid urine sediment filter device of claim 2, wherein, The outer diameter of the piston (2) is matched with the inner diameter of the extrusion cup (3), a plurality of annular grooves are arranged on the piston (2), a plurality of annular protrusions are arranged below the push rod (1), the annular grooves are matched with the annular protrusions, the piston (2) is inserted into the extrusion cup (3) after being connected to the annular protrusions on the push rod (1) through the annular grooves, and sealed extrusion is realized, so that the urine is quickly pushed through the through hole at the lower end of the extrusion cup (3) to the filter structure connected to the through hole.

4. The rapid urine sediment filter device of claim 3, wherein, The filter structure is detachably mounted on the through hole, the filter structure is a lower cover (5), the lower cover (5) has a middle through structure, the inner wall of the lower cover (5) has a groove, two silica gel gaskets (6) are arranged, and the plurality of layers of ultra-thin filter membranes (4) are arranged between the two silica gel gaskets (6).

5. The rapid urine sediment filtering device according to any one of claims 2 to 4, wherein, The lower parts of the push rod (1), the piston (2), and the extrusion cup (3) are matched inverted conical frustum structures, and the through hole is arranged at the bottom of the lower inverted conical frustum of the extrusion cup (3).

6. The rapid urine sediment filter device of claim 1, wherein, The ultra-thin filter membranes (4) are 3 layers.

7. The rapid urine sediment filter device of claim 1, wherein, After the urine is pushed through the filter structure by the extrusion structure, the waste liquid enters a waste liquid cup, and the waste liquid cup and the extrusion structure are integrally prepared or separately prepared.

8. The rapid urine sediment filter device of claim 2, wherein, A mesh structure is arranged on the through hole at the lower end of the extrusion cup (3).

9. The rapid urine sediment filter device of claim 4, wherein, A mesh structure is arranged at the lower end of the lower cover (5).

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