Trap type centrifugal tube for primary cell separation
By designing trap-type centrifuge tubes and using physical methods to separate primary cells, the problem of requiring additional solutions or antibodies in existing technologies has been solved, achieving simple and efficient cell separation that is suitable for primary cell separation of various tissue types.
Patent Information
- Application Number
- CN202520114562.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-01-17
- Publication Date
- 2026-01-30
- Estimated Expiration
- 2035-01-17
AI Technical Summary
Existing technologies require additional solutions or antigens/antibodies for primary cell separation, which leads to complex operations, high costs, and affects cell properties, making it impossible to achieve efficient batch separation.
Design a trap-type centrifuge tube, including a thick tube section, a conical section and a thin tube section. The thin tube section is provided with a pre-fabricated break and supporting wings along the height direction. Cells are separated by physical methods without the need for additional solutions or antigens/antibodies. The separation and extraction of cells are achieved by using centrifugal force.
It simplifies the operation process, reduces costs, improves separation efficiency and purity, maintains the original properties of cells, and is suitable for primary cell separation of various tissue types.
Smart Images
Figure CN223852614U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The utility model relates to centrifugal tube technical field especially, it is a kind of trap formula centrifugal tube for primary cell separation. BACKGROUND
[0002] Primary cell separation often mixes a variety of cell groups in tissue, and various separation methods are provided for cell separation to provide different levels, different purity separation and purification solutions according to different research and application requirements.Perco l l density gradient centrifugation mainly separates the required cells according to the suspension of cells, and the corresponding concentration of Perco l l separation solution is prepared using the method, and two or more different concentrations of Perco l l separation solution are prepared, so that the required cells are between the two Perco l l separation solutions after centrifugation, and then the middle layer cells are extracted for subsequent culture and other purposes.Immunomagnetic bead cell sorting technology mainly uses the principle of combining the antigens on the surface of cells with the specific antibodies on the magnetic beads, and the immunomagnetic beads are divided into positive selection method and negative selection method.Perco l l is mainly used for preliminary rough separation according to cell density (suspension), and the antibody is mainly used for fine separation of cells containing specific surface proteins.
[0003] Perco l l separation solution needs specific solvent when being prepared, and different brands of Perco l l cannot be unified in use and preparation.Perco l l separation solution has limited density range, and if the suspension density of cells is greater than the maximum limit or less than the minimum limit, the method cannot be used for separation.Using Perco l l separation method, other cells exist in the middle layer, and the required cells cannot be accurately separated.Perco l l itself is a gelatinous liquid, and the required cells need to be washed with culture medium after being separated, which will lose the required cells to some extent.Immunomagnetic bead separation technology needs to use magnetic beads and magnets suitable for cells, and needs to purchase corresponding antibodies on the surface of cells, and the separation technology and cost are relatively high, and the method is not suitable for batch separation of primary cells.In addition, the cost of immunomagnetic bead separation method is extremely high, and the load of the separated cells is extremely limited, and preliminary rough separation is usually needed to increase the separation purity and separation efficiency of the magnetic bead separation method.In view of the above reasons, the application provides a trap formula centrifugal tube for primary cell separation without the help of additional solution or antigen antibody. UTILITY MODEL CONTENT
[0004] The utility model aims at the problems in the background art, and provides a trap formula centrifugal tube for primary cell separation without the help of additional solution or antigen antibody.
[0005] The utility model discloses a technical scheme: a trap type centrifugal tube for primary cell separation, which comprises a centrifugal tube main body, wherein the centrifugal tube main body is sequentially provided with a thick tube part, a tapered part and a thin tube part from top to bottom.
[0006] The thin tube part is provided with a plurality of prefabricated breaking points in an array along the height direction thereof.
[0007] Optionally, the tapered part is used for connecting the thick tube part and the thin tube part, and the cross section of the tapered part is funnel-shaped.
[0008] Optionally, the bottom of the thin tube part is provided with a thin tube bottom with a semicircular cross section.
[0009] Optionally, a support wing is arranged between each of the prefabricated breaking points in an array on the outer wall of the thin tube part.
[0010] Optionally, the lateral width of the support wing is arranged in a decreasing manner from top to bottom along the length direction of the thin tube part, and a reverse triangular structure is formed at the bottom of the thin tube part.
[0011] Optionally, the centrifugal tube main body is detachably connected with the inner wall of a 15-ml external centrifugal tube, and the centrifugal tube main body is sleeved in the internal part of the external centrifugal tube.
[0012] Compared with the prior art, the utility model has the following beneficial technical effects:
[0013] The utility model does not need to use additional solution or antigen antibody, and only needs to add a certain cell concentration of cell suspension into the trap type centrifugal tube when separating cells to separate specific cells, and the operation is relatively simple, time-consuming is short, the efficiency of obtaining the rough separation cell group is high, and the cost is low.
[0014] The utility model is provided with the prefabricated breaking points, and different heights of the thin tube can be cut for separation, and each section of cell suspension in the thin tube is placed in a dish for culture.
[0015] The separation method used by the utility model is physical separation, which does not affect the physicochemical properties of cells, can maintain the characteristics of primary cells, can separate most of the primary cells of different tissue types, and if a large number of primary cells need to be separated, a few preliminary experiments can be performed first to determine the position of the required cells in the thin tube, and then the primary cells can be separated in batches.
[0016] In summary, the utility model has the advantages of relatively simple operation, very short time, no need of additional solution or antigen, and improvement of the influence of non-culture solution on cells. BRIEF DESCRIPTION OF DRAWINGS
[0017] Figure 1 A front view of the utility model is shown.
[0018] Figure 2 Fig. 1 is a perspective view of a centrifugal tube according to the present disclosure; Figure 1 Fig. 2 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line L-L; Fig. 3 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line A-A;
[0019] Fig. 4 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line B-B; Figure 3 Fig. 5 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line C-C; Figure 1 Fig. 6 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line D-D; Fig. 7 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line E-E;
[0020] Fig. 8 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line F-F; Figure 4 Fig. 9 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line G-G; Figure 1 Fig. 10 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line H-H; Fig. 11 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line I-I;
[0021] Fig. 12 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line J-J; Figure 5 Fig. 13 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line K-K; Figure 1 Fig. 14 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line L-L; Fig. 15 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line M-M;
[0022] Fig. 16 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line N-N; Figure 6 Fig. 17 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line O-O; Figure 1 Fig. 18 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line P-P; Fig. 19 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line Q-Q;
[0023] Fig. 20 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line R-R; Figure 7 Fig. 21 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line S-S; Figure 1 Fig. 22 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line T-T; Fig. 23 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line U-U;
[0024] Fig. 24 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line V-V; Fig. 25 is a cross-sectional view of the centrifugal tube of Fig. 1 taken along line W-W;
[0025] 1. Centrifugal tube body; 11. Thick tube portion; 12. Conical portion; 13. Pre-made break portion; 14. Support wing; 15. Thin tube portion; 16. Thin tube bottom. DETAILED DESCRIPTION
[0026] The technical solutions of the present disclosure will be described clearly and completely below with reference to the drawings. Obviously, the described embodiments are only part of the embodiments of the present disclosure, not all the embodiments.
[0027] The components of the embodiments of the present disclosure generally described and shown in the drawings herein can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present disclosure provided in the drawings is not intended to limit the scope of the claimed present disclosure, but only represents selected embodiments of the present disclosure.
[0028] Based on the embodiments in the present disclosure, all other embodiments obtained by those of ordinary skill in the art without creative labor are within the scope of protection of the present disclosure.
[0029] In the description of the present disclosure, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present disclosure and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present disclosure.
[0030] In the description of the present disclosure, it should be explained that, unless otherwise explicitly specified and limited, the terms "mount", "connect", "connection" should be understood in a broad sense, for example, it can be fixed connection, or detachable connection, or integrally connected; it can be mechanical connection, or electrical connection; it can be directly connected, or indirectly connected through intermediate medium, or the communication inside two elements. For those skilled in the art, the specific meaning of the above terms in the present disclosure can be understood according to the specific circumstances.
[0031] As Figures 1-7 The utility model provides a trap formula centrifugal tube for primary cell separation, including centrifugal tube main part 1, centrifugal tube main part 1 with 15ml's outer centrifugal tube inner wall clamping adaptation, the centrifugal tube main part 1 is set in the inside of outer centrifugal tube, and then is placed into centrifuge and carries out centrifugal work. Specifically, centrifugal tube main part 1 is sequentially provided with thick pipe part 11, taper part 12 and thin pipe part 15 from top to bottom, and the taper part 12 is used to communicate thick pipe part 11 and thin pipe part 15, and the cross section of taper part 12 is funnel-shaped.
[0032] Among them, thin pipe part 15 is provided with a plurality of prefabricated fracture 13 along its height direction array, and each layer prefabricated fracture 13 is provided with support side wing 14 arranged in array on the outer wall of thin pipe part 15, the horizontal width of support side wing 14 is arranged in decreasing order from top to bottom along the length direction of thin pipe part 15, and the bottom of thin pipe part 15 forms inverted triangular structure, and the bottom of thin pipe part 15 is provided with thin pipe bottom 16 with semicircular cross section.
[0033] The embodiment is specifically operated according to the following operation steps to separate cells:
[0034] 1, a certain amount of culture medium is added to the trap formula centrifugal tube, fills thin pipe part 15 and upper portion, and exhausts the bubble in the thin pipe, and the same culture medium is added to the taper part 12 at the upper portion of the centrifugal tube;
[0035] 2, taking 500 μL completely mixed cell suspension as an example, slightly tilt the trap formula centrifugal tube of the application, slowly add cell suspension to the upper layer of culture medium along the wall, try to not affect the lower layer, and mix the cell suspension in the original centrifugal tube after adding cell suspension each time;
[0036] 3, the trap formula centrifugal tube is sleeved into 15ml centrifugal tube, and is placed into centrifuge for centrifugation;
[0037] 4, after centrifugation, the liquid higher than thin pipe part 15 is sucked;
[0038] 5, then the trap formula centrifugal tube is taken out from 15ml centrifugal tube, and the thin pipe containing cells is pinched off from top to bottom at the prefabricated fracture 13 position by using forceps and cutting forceps.
[0039] 6. Put the tubule containing cells into different culture wells respectively, and flush the cells in the tubule into the culture dish with culture medium for culture;
[0040] 7. Repeat the above two steps until each section of cell suspension in the tubule is put into the dish for culture.
[0041] It is to be explained that the cells in the tissue are to be digested into single cells as much as possible, and cannot be aggregated into a group, otherwise the cell group will block the tubule opening during centrifugation, resulting in that the cells cannot enter the prefabricated fracture 13; the amount of cells required by the trap type centrifuge tube needs to reach or exceed the total volume of the tubule part 15 to meet the use conditions; when adding the cell suspension into the trap type centrifuge tube, it needs to be slowly added to the upper layer of the culture medium as much as possible without affecting the lower separation layer; after centrifugation, the cells should fill the prefabricated fracture 13 or only exist in the prefabricated fracture 13 to obtain a better separation effect; it is to be explained that the breakage of each layer of prefabricated fracture 13 needs to be adapted to the cutting forceps for cutting, and the cutting forceps are provided with an opening corresponding to the shape of the support flange 14, and after the support flange 14 is clamped, the corresponding prefabricated fracture 13 can be cut off; when harvesting the cells, the culture medium in the thick tube part 11 is first sucked, and then the tubule part 15 of different heights is cut off.
[0042] The above specific embodiments are only optional embodiments of the present application, and based on the technical scheme of the present application and the related inspiration of the above embodiments, the person skilled in the art can make various alternative improvements and combinations on the above specific embodiments.
Claims
1. A trap centrifuge tube for primary cell isolation, characterized by: The centrifugal tube body (1) is sequentially provided with a thick tube part (11), a tapered part (12) and a thin tube part (15) from top to bottom. The thin tube part (15) is provided with a plurality of prefabricated breaking points (13) along the height direction.
2. A trap centrifuge tube for primary cell isolation according to claim 1, characterized in that, The tapered part (12) is used for connecting the thick tube part (11) and the thin tube part (15), and the cross section of the tapered part (12) is a funnel structure.
3. A trap centrifuge tube for primary cell isolation according to claim 1, wherein, The bottom of the thin tube part (15) is provided with a thin tube bottom (16) with a semicircular arc cross section.
4. The trap centrifuge tube for primary cell isolation of claim 1, wherein, Each layer of the prefabricated breaking points (13) is provided with a support wing (14) arranged on the outer wall of the thin tube part (15).
5. A trap centrifuge tube for primary cell isolation according to claim 4, wherein, The lateral width of the support wing (14) is arranged in a decreasing manner from top to bottom along the length direction of the thin tube part (15), and forms an inverted triangular structure at the bottom of the thin tube part (15).
6. The trap centrifuge tube for primary cell isolation of claim 1, wherein, The centrifugal tube body (1) is clamped and matched with the inner wall of an external centrifugal tube, and is sleeved in the internal part of the external centrifugal tube.