Identification device for pneumonia infection source

Abstract

The utility model relates to the technical field of biology, and discloses a pneumonia infection source identification device which comprises a capturing part for capturing neutrophils, a negative pressure part for uniformly distributing blood or normal saline on the capturing part, and a conductive part for assisting the capturing part in electrifying, the observation part is used for observing the electrotaxis migration condition of the neutrophile granulocytes in the capturing part; the negative pressure part is also used for cleaning normal saline on the capturing part; the negative pressure part is pressed, so that blood or normal saline drips at the end, away from the negative pressure part, of the capturing part, then the negative pressure part is released, the negative pressure part adsorbs the blood or normal saline through the negative pressure effect, and the blood or normal saline is evenly distributed on the capturing part; the negative pressure piece is pressed and then released, the negative pressure piece enables the normal saline on the capturing piece to be away from the capturing piece through the negative pressure effect, and the normal saline on the capturing piece is cleaned. The pediatric pneumonia pathological typing device can accurately and quickly perform pathological typing on pediatric pneumonia in the early stage of pediatric pneumonia so as to assist clinical treatment.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biotechnology, specifically to a pneumonia infection source's discrimination device. BACKGROUND

[0002] Bronchopneumonia is a common disease and death cause of children, and the pathological types of bronchopneumonia mainly include bacterial and non-bacterial (such as viral), and the clinical pathological type of children's pneumonia is crucial, which further affects the clinical treatment scheme.

[0003] The existing clinical detection means of children's pneumonia pathological type mainly includes blood routine analysis, pathogen detection and imaging examination. Blood routine analysis detects the number and proportion of white blood cells (such as neutrophils) in blood, which is used to assist in judging the infection type, but it is easily interfered by factors such as children's age and immune state, and it is difficult to accurately judge; pathogen detection directly determines the infection source by isolating and culturing pathogens or amplifying nucleic acids, which has a long detection cycle and high economic cost; imaging examination directly observes lung lesions to evaluate the severity of pneumonia, which has the defects of low early pneumonia detection rate, difficulty in distinguishing pneumonia pathological types and radiation.

[0004] However, the above existing detection methods are difficult to meet the clinical needs of accurate and rapid pathological typing of children's pneumonia in the early stage of children's pneumonia due to insufficient sensitivity and specificity, limitations of functional analysis technology and low detection efficiency. CONTENT OF THE UTILITY MODEL

[0005] In order to overcome the shortcomings of the prior art, the purpose of the utility model is to provide a pneumonia infection source discrimination device, which can accurately and quickly classify the pathological type of children's pneumonia in the early stage of children's pneumonia, thereby assisting clinical treatment.

[0006] The technical scheme adopted by the utility model is as follows: a pneumonia infection source discrimination device, comprising an observation piece for observing the electrotaxis migration of neutrophils in the capture piece, and further comprising a capture piece for capturing neutrophils, a negative pressure piece for uniformly distributing blood or physiological saline on the capture piece, and a conductive piece for assisting the electrification of the capture piece, wherein the negative pressure piece is in communication with the capture piece and located at one end of the capture piece, and the negative pressure piece is also used for cleaning physiological saline on the capture piece.

[0007] Press the negative pressure piece, let the blood or physiological saline drop on the end of the capture piece away from the negative pressure piece, and then release the negative pressure piece. The negative pressure piece adsorbs the blood or physiological saline by negative pressure effect, so that the blood or physiological saline is uniformly distributed on the capture piece.

[0008] Press the negative pressure piece, and then release the negative pressure piece. The negative pressure piece makes the physiological saline on the capture piece away from the capture piece by negative pressure effect, so as to clean the physiological saline on the capture piece.

[0009] Principle of the technical solution:

[0010] Press the negative pressure piece, drop blood at the end of the capture piece away from the negative pressure piece, release the negative pressure piece, under the action of negative pressure, blood flows on the capture piece, and the neutrophils in the blood are captured by the capture piece, then press the negative pressure piece again, drop physiological saline (or PBS phosphate buffered saline) at the end of the capture piece away from the negative pressure piece, release the negative pressure piece, under the action of negative pressure, the physiological saline washes other cells except neutrophils and carries them to the end of the capture piece close to the negative pressure piece.

[0011] By the conductive piece, 3-12 volts of direct current is applied to both ends of the capture piece, and the movement and trajectory of the neutrophils are observed under the observation piece, if the neutrophils move quickly, it is judged that the type of pneumonia is bacterial pneumonia, and if the neutrophils move slowly, it is judged that the type of pneumonia is non-bacterial pneumonia.

[0012] Compared with the prior art, the beneficial effects of the utility model lie in:

[0013] The utility model discloses a capture piece and a negative pressure piece cooperate, can complete the capture of neutrophil, plus the setting of the conductive piece, the movement and trajectory of neutrophil are observed under the observation piece, so as to judge the type of pneumonia within 3-5 minutes according to the movement and trajectory of neutrophil, so that the pathological typing of pediatric pneumonia can be accurately and quickly determined in the early stage of pediatric pneumonia, thereby assisting clinical treatment.

[0014] As a preferred embodiment of the utility model, the capture piece includes a chip substrate, a flow-through groove is arranged in the chip substrate, an antibody layer for capturing neutrophils is arranged on the flow-through groove, and the flow-through groove can be used to accommodate the dropped blood or physiological saline.

[0015] Explanation: The antibody layer contains antibodies, which can be collagen I, extracellular matrix protein or laminin. By modifying the inner surface of the bottom of the flow-through groove with specific proteins, the specific proteins can effectively capture neutrophils without affecting the activity of neutrophils.

[0016] As a preferred embodiment of the utility model, the chip substrate includes a PDMS substrate and a glass slide, the PDMS substrate and the glass slide are permanently bonded, and the flow-through groove is located on the PDMS substrate.

[0017] Explanation: Permanent bonding means forming an inseparable interface through chemical or physical action.

[0018] As a preferred embodiment of the utility model, the negative pressure piece includes an air suction ball, the air suction ball is located at one end of the chip substrate, and the air suction ball is connected with the chip substrate and communicates with the flow-through groove.

[0019] Beneficial effects: the suction ball can make blood or physiological saline flow uniformly on the flow channel by negative pressure, or clean the physiological saline on the flow channel.

[0020] As the preferred embodiment of the utility model, the conductive part includes two electrodes respectively located at both ends of the antibody layer and connected with the inner surface of the bottom of the flow channel.

[0021] Beneficial effects: since the flow channel is located in the chip substrate, the electrode can assist the conductive inner surface of the bottom of the flow channel, so that the neutrophils captured by the antibody layer on the flow channel migrate electrotropically.

[0022] As the preferred embodiment of the utility model, the chip substrate is provided with a liquid storage pool in communication with the flow channel at both ends, and the liquid storage pool is used for containing the blood or physiological saline dropped therein, one of the liquid storage pools is located between the flow channel and the suction ball, and a one-way valve is arranged between the liquid storage pool close to the suction ball and the flow channel.

[0023] In the scheme, the suction ball is pressed, blood is dropped in the liquid storage pool away from the suction ball, the suction ball is released, under the action of negative pressure, the blood flows in the middle part of the flow channel, the neutrophils in the blood are captured by the antibodies of the antibody layer, the suction ball is pressed again, physiological saline (or PBS phosphate buffered saline) is dropped in the liquid storage pool away from the suction ball, the suction ball is released, under the action of negative pressure, the physiological saline washes and carries away other cells except neutrophils through the one-way valve to the liquid storage pool close to the suction ball.

[0024] Beneficial effects: the arrangement of the liquid storage pool and the one-way valve can prevent other cells except neutrophils and physiological saline from flowing back to the antibody layer, which affects the observation of the movement of neutrophils under a microscope.

[0025] As the preferred embodiment of the utility model, the chip substrate is provided with a first via hole at one end away from the suction ball, and the blood or physiological saline is dropped into one end of the flow channel through the first via hole.

[0026] Beneficial effects: the first via hole facilitates the dropping tube to pass through to drop the blood or physiological saline into one end of the flow channel.

[0027] As the preferred embodiment of the utility model, the chip substrate is provided with a second via hole at one end close to the suction ball, the two electrodes are respectively connected with the inner surface of the bottom of the corresponding end of the flow channel through the first via hole and the second via hole, and the electrode is attached to the inner wall of the second via hole.

[0028] Beneficial effects: the first via hole and the second via hole are convenient for setting electrodes, the electrodes are attached to the inner wall of the second via hole, the sealing property of the chip base can be ensured, specifically, the sealing property between the flow-through groove far from the one end of the air suction ball to the air suction ball can be ensured, and the blood can be smoothly adsorbed to the flow-through groove or the one end of the flow-through groove close to the air suction ball by pressing and releasing the air suction ball. BRIEF DESCRIPTION OF DRAWINGS

[0029] Figure 1 is the structure schematic view of the pneumonia infection source identification device of the utility model;

[0030] Figure 2 is the structure schematic view of the partial pneumonia infection source identification device of the utility model;

[0031] Figure 3 is the structure schematic view of the partial pneumonia infection source identification device of the utility model from another angle. DETAILED DESCRIPTION

[0032] The typical embodiments embodying the features and advantages of the present application will be described in detail in the following description. It should be understood that the present application can have various changes on different embodiments, which all do not deviate from the scope of the present application, and the description and drawings in essence are used as illustration, not to limit the present application.

[0033] In the description of the present application, the terms "first", "second", "one side" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, which is only for the convenience of describing the present application and simplifying the description, and does not indicate or imply that the indicated structure must have a particular orientation, be constructed and operated in a particular orientation, therefore cannot be understood as a limitation on the present application.

[0034] The present application will be described in detail below with reference to the drawings and in combination with the embodiments.

[0035] The reference signs include: flow-through groove 1, antibody layer 2, air suction ball 3, electrode 4, PDMS substrate 5, glass slide 6, one-way valve 7, first via hole 8, second via hole 9.

[0036] As shown in the drawings, Figure 1 The pneumonia infection source identification device includes an observation member for observing the electrotaxis migration of neutrophils in the capture member, a capture member for capturing neutrophils, a negative pressure member for uniformly distributing blood or physiological saline on the capture member, and a conductive member for assisting the electrification of the capture member, the negative pressure member is in communication with the capture member and located at one end of the capture member, and the negative pressure member is also used for cleaning physiological saline on the capture member.

[0037] Press the negative pressure element to let blood or saline drip onto the end of the capture element away from the negative pressure element, then release the negative pressure element. The negative pressure element will absorb the blood or saline through negative pressure, allowing the blood or saline to be evenly distributed on the capture element.

[0038] Press the negative pressure element and then release it. The negative pressure element will use negative pressure to move the saline solution on the capture element away from the capture element, thus cleaning the saline solution on the capture element.

[0039] In this embodiment, the observation device can be a scanning electron microscope, a high-speed camera, or an optical coherence tomography (OCT).

[0040] In this embodiment, the capturing element includes a chip substrate, such as... Figure 2 As shown, the chip substrate is a PDMS-glass microfluidic chip. The chip substrate includes a PDMS substrate 5 and a glass slide 6. The PDMS substrate 5 and the glass slide 6 are permanently bonded, that is, the upper surface of the PDMS substrate 5 and the lower surface of the glass slide 6 are tightly attached. In this embodiment, the PDMS substrate is a thin film or substrate made of polydimethylsiloxane.

[0041] like Figure 3 As shown, a flow channel 1 is provided on the PDMS substrate 5. In this embodiment, the flow channel 1 is a straight microchannel. An antibody layer 2 is provided on the flow channel 1. The bottom inner surfaces of both ends of the flow channel 1 are connected to conductive components. In this embodiment, the conductive components are used to assist the capture component in being energized. The conductive components include two electrodes 4. Both ends of the flow channel 1 can be used to contain blood or saline. In this embodiment, both ends of the chip substrate are provided with reservoirs communicating with the flow channel 1. The reservoirs are used to contain blood or saline. One end of the chip substrate is connected to a negative pressure component communicating with the flow channel 1. In this embodiment, the negative pressure component is used to make the blood or saline evenly distributed on the capture component. The negative pressure component includes a suction balloon 3. One reservoir is located between the flow channel 1 and the suction balloon 3. A one-way valve 7 is provided between the reservoir near the suction balloon 3 and the flow channel 1.

[0042] like Figure 2 As shown, in this embodiment, a first through hole 8 is provided on the end of the glass slide 6 away from the suction balloon 3. The first through hole 8 is connected to the liquid storage tank. Blood or saline solution drips into the flow channel 1 through the first through hole 8 and the liquid storage tank. A second through hole 9 is provided on the end of the glass slide 6 near the suction balloon 3.

[0043] In this embodiment, the two electrodes 4 are connected to the inner surface of the bottom of the corresponding end of the flow channel 1 through the first through hole 8 and the second through hole 9, respectively, and the electrodes 4 are attached to the inner wall of the second through hole 9.

[0044] In this embodiment, the suction bulb can be an ear syringe.

[0045] This invention utilizes a flow channel, antibody layer, and suction balloon located within the chip substrate to capture neutrophils. With the addition of two electrodes, the movement and trajectory of neutrophils can be observed under a microscope. Based on the movement and trajectory of neutrophils, the type of pneumonia can be determined within 3-5 minutes. This allows for accurate and rapid pathological classification of pediatric pneumonia in its early stages, thus assisting in clinical treatment.

[0046] In this embodiment, pressing the suction balloon 3 drips blood into a reservoir away from the suction balloon 3. Releasing the suction balloon 3 causes blood to flow in the middle of the flow channel 1 under negative pressure. Neutrophils in the blood are captured by antibodies in the antibody layer 2. Pressing the suction balloon 3 again drips physiological saline (or PBS phosphate buffered saline) into a reservoir away from the suction balloon 3. Releasing the suction balloon 3 (with varying pressure each time) causes physiological saline to wash away cells other than neutrophils under negative pressure and carry them through the one-way valve 7 to a reservoir near the suction balloon 3.

[0047] By applying a direct current of 3 to 12 volts to the bottom of the flow channel 1 through two electrodes 4, the movement and trajectory of neutrophils are observed under a microscope. If the neutrophils move rapidly, the pneumonia type is determined to be bacterial pneumonia. If the neutrophils move slowly, the pneumonia type is determined to be non-bacterial pneumonia, such as viral pneumonia.

[0048] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. A device for identifying a source of pneumonia infection, comprising an observation element for observing the electrotactic migration of neutrophils in a capture element, characterized in that: It also includes a capture device for capturing neutrophils, a negative pressure device for evenly distributing blood or saline solution on the capture device, and a conductive device for assisting the capture device in energizing. The negative pressure device is connected to the capture device and is located at one end of the capture device. The negative pressure device is also used to clean the saline solution on the capture device. Press the negative pressure element to let blood or saline drip onto the end of the capture element away from the negative pressure element, then release the negative pressure element. The negative pressure element will absorb the blood or saline through negative pressure, allowing the blood or saline to be evenly distributed on the capture element. Press the negative pressure element and then release it. The negative pressure element will use negative pressure to move the saline solution on the capture element away from the capture element, thus cleaning the saline solution on the capture element.

2. The device for identifying pneumonia infection sources according to claim 1, characterized in that: The capture device includes a chip substrate with a flow channel inside. The flow channel is provided with an antibody layer for capturing neutrophils, and the two ends of the flow channel can be used to accommodate dripped blood or saline.

3. The device for identifying pneumonia infection sources according to claim 2, characterized in that: The chip substrate includes a PDMS substrate and a glass slide, which are permanently bonded together, and the flow channel is located on the PDMS substrate.

4. The device for identifying pneumonia infection sources according to claim 2, characterized in that: The negative pressure component includes a suction ball, which is located at one end of the chip substrate and is connected to the chip substrate and communicates with the flow channel.

5. The device for identifying pneumonia infection sources according to claim 4, characterized in that: The conductive element includes two electrodes located at both ends of the antibody layer and connected to the inner surface of the bottom of the flow channel.

6. The device for identifying pneumonia infection sources according to claim 4, characterized in that: The chip substrate has liquid storage tanks at both ends that are connected to the flow channel. The liquid storage tanks are used to hold dripped blood or saline solution. One of the liquid storage tanks is located between the flow channel and the suction balloon. A one-way valve is provided between the liquid storage tank near the suction balloon and the flow channel.

7. The device for identifying pneumonia infection sources according to claim 5, characterized in that: The chip substrate has a first through hole at the end away from the suction balloon, through which blood or saline solution is dripped into one end of the flow channel.

8. The device for identifying pneumonia infection sources according to claim 7, characterized in that: The chip substrate has a second via on one end near the suction ball. The two electrodes are connected to the inner surface of the bottom of the corresponding end of the flow groove through the first via and the second via, respectively. The electrodes are in contact with the inner wall of the second via.

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