Oral bacteria culture equipment

Abstract

The utility model relates to oral bacteria culture equipment, which comprises a box body, a culture chamber and a filter screen, the partition plates are positioned in the culture chamber so as to divide the culture chamber into a plurality of culture areas; the deformation assembly comprises a deformation part and an air supply part; the deformation parts and the partition plates are arranged in a one-to-one correspondence mode, the deformation parts are arranged on the partition plates, and the air supply part drives the deformation parts to deform towards the side close to or away from the culture area by supplying air or exhausting air; and the breathing simulation device is mounted on the box body, is used for simulating the oral breathing process, and has the advantages of being capable of simulating the dynamic oral environment and further improving the accuracy of oral bacterial culture.

Description

Technical Field

[0001] This utility model relates to the technical field of bacterial culture equipment, and in particular to an oral bacterial culture device. Background Technology

[0002] In recent years, with the deepening of research in microecology, increasing evidence has shown that the balance of oral microbiota is closely related to oral diseases and even systemic diseases. The oral cavity is a complex micro-ecosystem, and the dynamic changes in its internal environment directly affect the composition and metabolic activities of the microbiota.

[0003] Traditional artificial culture methods are often carried out under static gas conditions, which makes it difficult to realistically simulate dynamic characteristics such as airflow changes (such as airflow changes during respiration) and spatial changes (such as changes in the volume of the oral cavity when the mouth is closed and open). This results in significant differences between the in vitro cultured microbiota and the microbiota in the real oral environment. Utility Model Content

[0004] The technical problem to be solved by this invention is to overcome the defects in the prior art, thereby providing an oral bacteria incubator.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] An oral bacteria culture device, comprising:

[0007] The container contains a culture chamber;

[0008] A partition, located within the culture chamber, divides the culture chamber into several culture zones;

[0009] A deformation component includes a deformation part and an air supply part; the deformation part is disposed in a one-to-one correspondence with the partition and is disposed on the partition; the air supply part drives the deformation part to deform toward the side closer to or away from the culture area by supplying or venting air.

[0010] A breathing simulation device, installed on the housing, is used to simulate the oral breathing process.

[0011] Preferably, the partition is detachably installed in the culture chamber;

[0012] The deformable part includes a gas chamber and an elastic diaphragm;

[0013] The gas chamber is located on the side of the partition facing the culture area and has an opening;

[0014] The elastic diaphragm seals the opening to separate the gas chamber from the culture area.

[0015] Preferably, it also includes a support plate and an abutment ring;

[0016] The support plate is detachably installed in the culture chamber and is symmetrically distributed about the partition. The support plate has a slot that cooperates with the partition. The partition is provided with a limiting strip to prevent the support plate from tilting.

[0017] One end of the abutting ring abuts against the wall of the insertion groove, and the other side abuts against the elastic diaphragm. When the support plate and the partition are inserted, the elastic diaphragm is pressed against the opening.

[0018] Preferably, the gas supply unit includes a gas supply pipe, a gas distribution chamber, and a gas supply fan;

[0019] The gas supply pipe is connected to the partition plate one by one, and one end is connected to the gas distribution chamber and the other end is connected to the gas chamber.

[0020] The gas distribution chamber is connected to the gas output end of the gas supply fan;

[0021] The gas distribution chamber is also provided with an exhaust port, and a plug can be detachably installed on the exhaust port.

[0022] Preferably, the gas supply pipe is fixedly connected to its corresponding partition, and the other end passes through the side wall of the box and is inserted into the gas distribution chamber.

[0023] Preferably, the breathing simulation device includes a first end, a second end, an inhalation component, and an exhalation component;

[0024] The first end and the second end are symmetrically arranged about the culture chamber;

[0025] The first end has a first chamber that connects the culture area and the external space, and the opening of the first chamber has a removable baffle.

[0026] The second end has a second chamber, which connects the input end of the inhalation component and the culture area, and the second chamber also connects the output end of the exhalation component and the culture area.

[0027] Preferably, the air intake assembly includes an air intake fan and an air intake pipe, the air intake pipe connecting the second chamber and the air intake fan;

[0028] and / or;

[0029] The exhalation assembly includes an exhalation blower, an exhalation tube, and an exhaled gas chamber (443); the input end of the exhalation blower is connected to the exhaled gas chamber (443), and the output end of the exhalation blower is connected to the second chamber through the exhalation tube.

[0030] Preferably, a support plate is provided between the first chamber and the culture chamber, and between the second chamber and the culture chamber;

[0031] The support plates are symmetrically distributed about the partition and have insertion slots that cooperate with the partition.

[0032] The support plate is provided with equalization ports that correspond one-to-one with the culture areas.

[0033] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0034] This invention provides an oral bacterial culture device that, through the inclusion of a respiratory simulation device, can simulate the oral breathing process, thereby effectively simulating changes in gas flow within the oral environment. This provides a more realistic simulation of the oral bacterial culture environment, thus improving the accuracy of bacterial culture. Furthermore, the inclusion of a deformation component allows the deformable part to deform towards or away from the culture area, effectively simulating the relatively large internal space of the oral cavity when open and the smaller internal space when closed, further enhancing the accuracy of bacterial culture. In other words, the combination of the deformation component and the respiratory simulation device more realistically simulates the dynamic characteristics of airflow and spatial changes within the oral cavity, thereby providing a more realistic simulation of the oral environment and improving the accuracy of oral bacterial culture. Attached Figure Description

[0035] To more clearly illustrate the specific embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the specific embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0036] Figure 1 This is a schematic diagram of one embodiment of the present invention.

[0037] Figure 2 for Figure 1 A cross-sectional diagram from another perspective.

[0038] Figure 3 for Figure 2 An enlarged view of position D1 in the middle.

[0039] Figure 4 for Figure 2 A schematic diagram showing the positions of the partition plate, support plate, and deformation section.

[0040] Figure 5 for Figure 4 A partial cross-sectional diagram.

[0041] Figure 6 This is a schematic diagram of the gas flow direction during the operation of the breathing simulation device.

[0042] Figure 7 for Figure 1 A cross-sectional view of the intake component.

[0043] Figure 8 for Figure 7 An enlarged view of position D2 in the middle.

[0044] Explanation of reference numerals in the attached figures:

[0045] 1. Box body; 11. Culture area; 12. Door panel; 2. Partition; 21. Limiting strip; 3. Deformation component; 31. Deformation part; 311. Gas chamber; 312. Elastic diaphragm; 32. Gas supply part; 321. Gas supply pipe; 322. Gas distribution chamber; 3221. Exhaust port; 3222. Blocking block; 323. Gas supply fan; 4. Breathing simulation device; 41. First end; 411. First chamber; 412. Baffle; 42. Second end; 421. Second chamber; 43. Inhalation component; 431. Inhalation fan; 432. Inhalation pipe; 44. Exhalation component; 441. Exhalation fan; 442. Exhalation pipe; 443. Exhaled gas box; 5. Support plate; 51. Insertion slot; 52. Flow equalization port; 6. Abutment ring. Detailed Implementation

[0046] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0047] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0048] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0049] See Figures 1 to 7 This utility model provides an oral bacteria culture device, including a housing 1, a partition 2, a deformation component 3, and a breathing simulation device 4. The housing 1 has a culture chamber, and the partition 2 is located in the culture chamber to divide the culture chamber into several culture areas 11. The deformation component 3 includes a deformation part 31 and an air supply part 32. The deformation part 31 is arranged one-to-one with the partition 2 and is disposed on the partition 2. The air supply part 32 drives the deformation part 31 to deform toward or away from the culture area 11 by supplying or venting air. The breathing simulation device 4 is installed on the housing 1 and is used to simulate the oral breathing process.

[0050] It is easy to understand that in the above scheme, the breathing simulation device 4 can simulate the oral breathing process, thereby effectively simulating changes in gas flow in the oral environment and more realistically simulating the culture environment of oral bacteria, thus improving the accuracy of bacterial culture. The deformation component 3 allows the deformation part 31 to deform towards or away from the culture area 11, effectively simulating the relatively large internal space of the oral cavity when it is open and the relatively small internal space when it is closed, thus improving the accuracy of bacterial culture. In other words, the combination of the deformation component 3 and the breathing simulation device 4 more realistically simulates the dynamic characteristics of airflow and spatial changes in the oral cavity, thereby more realistically simulating the oral environment and improving the accuracy of oral bacterial culture.

[0051] See Figures 2 to 5 The partition 2 can be detachably installed in the culture chamber, and the deformable part 31 is provided on the partition 2.

[0052] Specifically, the partition 2 has three pieces, dividing the culture chamber into three culture zones 11. One end of the box body 1 has an opening that connects the culture chamber to the outside space. The opening has a door 12 that is rotatably connected to the box body 1. Under normal conditions, the door 12 closes to the opening of the box body 1 to seal the culture chamber. When it is necessary to remove the partition 2, the door 12 is opened so that the partition 2 can be removed from the culture chamber.

[0053] Furthermore, the deformable part 31 includes a gas chamber 311 and an elastic diaphragm 312; the gas chamber 311 is disposed on the side of the partition 2 facing the culture area 11 and has an opening; the elastic diaphragm 312 is sealed at the opening to separate the gas chamber 311 from the culture area 11.

[0054] It is worth noting that during the deformation of the elastic diaphragm 312, the distance between it and the cultured bacteria decreases, making it easier for bacteria to adhere to the elastic diaphragm 312. If the equipment is not thoroughly cleaned during two adjacent culture processes, cross-contamination can easily occur. By detachably installing the partition 2 in the culture chamber and setting the deformation part 31 on the partition 2, it is easier to disassemble the partition 2 and the deformation part 31 as a whole, thus facilitating the cleaning of the equipment.

[0055] It is easy to understand that the uppermost partition 2 abuts against the top of the culture chamber, which is mainly to ensure the installation of the deformable part 31 corresponding to the uppermost culture area 11, thereby ensuring that all deformable parts 31 can be effectively disassembled.

[0056] There are various sealing methods between the elastic diaphragm 312 and the opening of the gas chamber 311, such as bonding and pressing.

[0057] Specifically, see Figures 2 to 5 In this embodiment, the oral bacteria culture device also includes a support plate 5; wherein the support plate 5 is detachably installed in the culture chamber and is symmetrically distributed about the partition 2. The support plate 5 has an insertion groove 51 that cooperates with the partition 2, and the partition 2 is provided with a limiting strip 21 to restrict the tilting of the support plate 5.

[0058] It is easy to understand that the partition 2 and the support plate 5 are inserted perpendicularly to each other. Through the insertion and cooperation of the two, the partition 2 and the support plate 5 can be disassembled and assembled, which is convenient for cleaning the equipment, and the stability of the installation of the partition 2 and the support plate 5 can be guaranteed.

[0059] Furthermore, the oral bacterial culture device also includes an abutment ring 6, one end of which abuts against the wall of the insertion groove 51, and the other side abuts against the elastic diaphragm 312. When the support plate 5 and the partition plate 2 are inserted, the elastic diaphragm 312 is pressed against the opening.

[0060] It is easy to understand that by pressing the elastic diaphragm 312 to the opening through the abutment ring 6, the elastic diaphragm 312 can seal the gas chamber 311, so that the elastic diaphragm 312 can deform when the gas pressure in the gas chamber 311 increases. It also facilitates the separation of the elastic diaphragm 312 from the partition plate 2, which is beneficial for the replacement or cleaning of the elastic diaphragm 312.

[0061] See Figure 2 and Figure 3The gas supply unit 32 includes a gas supply pipe 321, a gas distribution chamber 322, and a gas supply fan 323; wherein, the gas supply pipe 321 is connected to the partition 2 in a one-to-one correspondence, and one end is connected to the gas distribution chamber 322, and the other end is connected to the gas chamber 311; the gas distribution chamber 322 is connected to the gas output end of the gas supply fan 323.

[0062] Furthermore, the gas distribution chamber 322 is also provided with an exhaust port 3221, and a blocking block 3222 is detachably installed on the exhaust port 3221. When the blocking block 3222 is removed, the gas chamber 311 can be vented through the gas supply pipe 321, the gas distribution chamber 322, and the exhaust port 3221, thereby reducing the pressure inside the gas chamber 311 and causing the elastic diaphragm 312 to deform (i.e., reset) away from the culture area 11. Of course, in other embodiments, the gas distribution chamber 322 can also be connected to an air pump, and the gas chamber 311 can be vented by the air pump.

[0063] Furthermore, the gas supply pipe 321 is fixedly connected to its corresponding partition 2, and the other end passes through the side wall of the chamber 1 and is inserted into the gas distribution chamber 322. It can be understood that the central axis of the gas supply pipe 321 coincides with the direction in which the partition 2 is taken out of the culture chamber, that is, the Y direction in the figure. This setting can not only realize the connection between the gas supply pipe 321 and the gas distribution chamber 322 and the partition 2, but also realize the positioning and installation of the partition 2 with the help of the gas supply pipe 321.

[0064] It should be understood that when the elastic diaphragm 312 needs to deform toward the side closer to the culture zone 11, the vent 3221 is blocked by the block block 3222, and the fan supplies gas to the gas chamber 311 through the gas distribution chamber 322 and the gas supply pipe 321, so that the pressure inside the gas chamber 311 increases and the elastic diaphragm 312 deforms toward the culture zone 11; when the elastic diaphragm 312 needs to be reset, the block block 3222 is removed from the vent 3221, and the gas in the gas chamber 311 is discharged from the gas supply pipe 321, the gas distribution chamber 322 and the vent 3221, the pressure inside the gas chamber 311 decreases, and the elastic diaphragm 312 resets.

[0065] It should also be understood that the deformation of the elastic diaphragm 312 can be set according to the air volume of the fan. When the elastic diaphragm 312 is deformed to the preset state, the fan can be turned off so that the elastic diaphragm 312 is maintained in the preset state.

[0066] See Figure 1 ,as well as Figures 6 to 8The breathing simulation device 4 includes a first end 41, a second end 42, an inhalation component 43, and an exhalation component 44; wherein the first end 41 and the second end 42 are symmetrically arranged about the culture chamber; the first end 41 has a first chamber 411 that connects the culture area 11 and the external space, and the opening of the first chamber 411 has a removable baffle 412; the second end 42 has a second chamber 421 that connects the input end of the inhalation component 43 and the culture area 11, and the second chamber 421 also connects the output end of the exhalation component 44 and the culture area 11.

[0067] Furthermore, the air intake assembly 43 includes an air intake fan 431 and an air intake pipe 432, the air intake pipe 432 connecting the second chamber 421 and the air intake fan 431;

[0068] Furthermore, the exhalation assembly 44 includes an exhalation blower 441, an exhalation tube 442, and an exhaled gas chamber 443; the input end of the exhalation blower 441 is connected to the exhaled gas chamber 443, and the output end of the exhalation blower 441 is connected to the second chamber 421 through the exhalation tube 442.

[0069] Understandably, during simulated inhalation, the first chamber 411 is connected to the outside space, the inhalation fan 431 is turned on, and the exhalation fan 441 is turned off, allowing outside air to enter the culture chamber from the first chamber 411, and then enter the inhalation fan 431 from the second chamber 421 and the inhalation tube 432, and be discharged from the output end of the inhalation fan 431, thus simulating the inhalation process (see gas path). Figure 6 (Indicated by the solid arrow in the middle); When simulating exhalation, the first chamber 411 is connected to the outside space, the inhalation fan 431 is turned off, and the exhalation fan 441 is turned on. The gas stored in the gas chamber enters the second chamber 421 through the exhalation fan 441 and the exhalation tube 442, and then enters the culture chamber from the second chamber 421, before being discharged through the first chamber 411, thus simulating the exhalation process (see gas flow path). Figure 6 (Direction indicated by the dashed arrow).

[0070] It is worth noting that during inhalation, the second chamber 421 is disconnected from the expiratory fan 441, while during exhalation, the second chamber 421 is disconnected from the inhalation fan 431, to ensure that the gas flows along... Figure 6 The flow is in the direction shown.

[0071] Furthermore, "external space" refers to the space relative to the culture chamber, the first chamber 411, and the second chamber 421. It can be the external environment or an external gas chamber system, such as a gas chamber system capable of supplying gases with preset parameters. The specific configuration can be tailored to the actual situation. For example, when the first chamber 411 is directly connected to the external environment, the intake component 43, when simulating the intake process, draws in ambient air. If temperature and humidity control is required, a corresponding temperature and humidity control chamber can be installed at the entrance of the first chamber 411 to regulate air parameters. When the first chamber 411 is connected to an external gas chamber system capable of supplying gases with preset parameters, the intake component 43, when simulating the intake process, draws in gas supplied by the gas chamber system with preset parameters. For example, the simulated intake gas parameters could be set as follows: O2:CO2:N2 = 21%:0.03% (can be zero):79%, temperature = 25℃, and relative humidity = 50%. The gas chamber system capable of supplying gases with preset parameters can be an existing system and is not an improvement of this application; therefore, it will not be elaborated upon. It should also be understood that if an external gas chamber system is connected, the inhalation assembly 43 and the exhalation assembly 44 are relatively independent, that is, the first chamber 411 and the second chamber 421 are each divided into two parts connected to the inhalation assembly 43 and the exhalation assembly 44.

[0072] Furthermore, a support plate 5 is provided between the first chamber 411 and the culture chamber, and between the second chamber 421 and the culture chamber; the support plate 5 is symmetrically distributed about the partition 2 and has an insertion groove 51 that cooperates with the partition 2; the support plate 5 is provided with a flow equalization port 52 corresponding to the culture area 11.

[0073] It is easy to understand that by interlocking the support plate 5 and the partition plate 2 to form the corresponding culture area 11, and with the flow equalization port 52 set on the support plate 5, it is beneficial to the overall cleanliness and can ensure the uniformity of the gas delivered to each culture area.

[0074] Specifically, CFD simulations can be used to verify the uniformity of airflow distribution and ensure that the surface wind speed difference in each cultivation zone is less than 5%.

[0075] This embodiment also provides a method for culturing oral bacteria suitable for the above-mentioned oral bacterial culture equipment, including the following steps:

[0076] During the first preset time period, the culture area 11 is kept sealed (that is, the breathing simulation device 4 is turned off and the baffle 412 is installed so that the first chamber 411 is kept sealed from the external environment), and the deformation part 31 is driven to deform toward the corresponding culture area 11 to simulate the closed state of the oral cavity at night.

[0077] During the second preset time period, the cultivation area is kept in a state where gas can flow (that is, the baffle 412 is removed, and the first chamber 411 is kept in a state of communication with the outside space), and the breathing simulation device 4 is used to simulate the oral breathing process.

[0078] Furthermore, the process of simulating oral breathing using the breathing simulation device 4 specifically includes the following steps:

[0079] The inhalation assembly 43 is turned on and the exhalation assembly 44 is turned off, so that outside air or gas with the first preset parameters passes through the first chamber 411, the cultivation area, the second chamber 421 and the inhalation assembly 43 in sequence, and is discharged from the output end of the inhalation assembly 43 to simulate the inhalation process.

[0080] The inhalation assembly 43 is closed and the exhalation assembly 44 is opened, so that the gas with the second preset parameters in the exhalation gas box 443 passes through the exhalation fan 441, the exhalation tube 442, the second chamber 421, the cultivation area and the first chamber 411 in sequence, and is discharged from the first chamber 411 to simulate the exhalation process.

[0081] The uniformity of airflow distribution was verified through CFD simulation to ensure that the surface wind speed difference was <5% during simulated oral respiration in each culture layer.

[0082] The process of inhalation and exhalation is simulated alternately, with each inhalation lasting 2 seconds and each exhalation lasting 3 seconds.

[0083] In addition, it is worth noting that the parameters of the inhaled gas can be set as O2:CO2:N2 = 21%:0.03% (can be zero):79%, temperature = 25℃, and relative humidity = 50%; the parameters of the exhaled gas can be set as O2:CO2:N2 = 16%:5%:79%, temperature = 37℃, and relative humidity = 100%.

[0084] The above embodiments are merely preferred embodiments of this utility model and should not be construed as limiting the scope of protection of this utility model. Any non-substantial changes and substitutions made by those skilled in the art based on this utility model shall fall within the scope of protection claimed by this utility model.

Claims

1. An oral bacterial culture device, characterized in that, include: Box (1), with a culture chamber; A partition (2) is located in the culture chamber to divide the culture chamber into several culture zones (11). The deformation component (3) includes a deformation part (31) and an air supply part (32); the deformation part (31) is provided in a one-to-one correspondence with the partition (2) and is provided on the partition (2); the air supply part (32) drives the deformation part (31) to deform toward or away from the culture area (11) by supplying or venting air. A breathing simulation device (4) is installed on the housing (1) and is used to simulate oral breathing.

2. The oral bacterial culture device according to claim 1, characterized in that, The partition (2) can be detachably installed in the culture chamber; The deformable part (31) includes a gas chamber (311) and an elastic diaphragm (312). The gas chamber (311) is located on the side of the partition (2) facing the culture area (11) and has an opening; The elastic diaphragm (312) is sealed at the opening to separate the gas chamber (311) from the culture area (11).

3. The oral bacterial culture device according to claim 2, characterized in that, It also includes a support plate (5) and an abutment ring (6); The support plate (5) is detachably installed in the culture chamber and is symmetrically distributed about the partition (2). The support plate (5) has a plug groove (51) that cooperates with the partition (2). The partition (2) is provided with a limiting strip (21) to restrict the tilting of the support plate (5). One end of the abutting ring (6) abuts against the wall of the insertion groove (51), and the other side abuts against the elastic diaphragm (312). When the support plate (5) is inserted into the partition plate (2), the elastic diaphragm (312) is pressed against the opening.

4. The oral bacterial culture device according to claim 2, characterized in that, The gas supply unit (32) includes a gas supply pipe (321), a gas distribution chamber (322), and a gas supply fan (323). The gas supply pipe (321) is connected to the partition (2) one by one, and one end is connected to the gas distribution chamber (322), and the other end is connected to the gas chamber (311); The gas distribution chamber (322) is connected to the gas output end of the gas supply fan (323); The gas distribution chamber (322) is also provided with an exhaust port (3221), and a plug (3222) can be detachably installed on the exhaust port (3221).

5. The oral bacterial culture device according to claim 4, characterized in that, The gas supply pipe (321) is fixedly connected to its corresponding partition (2), and the other end passes through the side wall of the box (1) and is inserted into the gas distribution chamber (322).

6. The oral bacterial culture device according to claim 1, characterized in that, The breathing simulation device (4) includes a first end (41), a second end (42), an inhalation component (43), and an exhalation component (44). The first end (41) and the second end (42) are symmetrically arranged about the culture chamber; The first end (41) has a first chamber (411) that connects the culture area (11) and the external space, and the opening of the first chamber (411) has a removable baffle (412). The second end (42) has a second chamber (421) which is connected to the input end of the inhalation assembly (43) and the culture area (11). The second chamber (421) is also connected to the output end of the exhalation assembly (44) and the culture area (11).

7. The oral bacterial culture device according to claim 6, characterized in that, The air intake assembly (43) includes an air intake fan (431) and an air intake pipe (432), the air intake pipe (432) connecting the second chamber (421) and the air intake fan (431). and / or; The exhalation assembly (44) includes an exhalation fan (441), an exhalation tube (442), and an exhaled gas chamber (443); the input end of the exhalation fan (441) is connected to the exhaled gas chamber (443), and the output end of the exhalation fan (441) is connected to the second chamber (421) through the exhalation tube (442).

8. The oral bacterial culture device according to claim 6, characterized in that, A support plate (5) is provided between the first chamber (411) and the culture chamber, and between the second chamber (421) and the culture chamber. The support plate (5) is symmetrically distributed about the partition plate (2) and has a plug groove (51) that cooperates with the partition plate (2). The support plate (5) is provided with flow equalization ports (52) that correspond one-to-one with the culture area (11).

Images