An adaptive fixation bone marrow assembly separation device

Abstract

This invention discloses an adaptive fixation bone marrow assembly separation device, comprising a separation tube with a gradually decreasing inner bottom space. A core is assembled inside the separation tube, and the core has multiple evenly distributed fixation channels for fixing the bone. After the outer wall of the core is in close contact with the inner wall of the separation tube, a liquid-carrying area is formed in the upper part of the separation tube, which is used to fill the irrigation fluid. When the core is inserted into the bottom of the separation tube, it is compressed and deformed. Simultaneously, the fixation channels deform synchronously and clamp the fixed bone inside. This invention uses a pressure assembly method to assemble the core in the gradually decreasing space at the bottom of the separation tube, causing the core to be compressed and deformed, which prompts the fixation channels to adaptively adjust the clamping force on the bone according to the centrifugation speed. A liquid-carrying area is formed above the core to fill the bone marrow cavity with irrigation fluid during centrifugation, achieving integration of centrifugal separation and irrigation separation, thus improving the separation effect.

Description

Technical Field

[0001] This utility model relates to the field of biological experimental equipment technology, specifically to an adaptive fixation bone marrow assembly separation device. Background Technology

[0002] In biological experiments, bone marrow is primarily obtained from the medullary cavity of long bones, and from the cancellous spaces of flat and irregular bones in animals. Current bone marrow separation methods mainly employ centrifugation. When separating bone marrow from bone using centrifugation, multiple severed bone fragments are typically placed in a small inner centrifuge tube, which is then nested inside a larger outer centrifuge tube. A hole is drilled at the bottom of the inner centrifuge tube. The inner and outer tubes are then sealed and placed at an angle in a centrifuge. During centrifugation, the bone marrow within the bone begins to separate from the inner wall of the bone medullary cavity due to centrifugal force, flowing out from the lower end of the bone. The flowing bone marrow is then thrown off the bone by centrifugation and falls onto the inner wall of the inner centrifuge tube, where it accumulates towards the bottom and eventually passes through the hole to be collected at the bottom of the outer centrifuge tube.

[0003] While this method of nested centrifuge tubes for bone marrow separation can reduce the contact area between bone marrow fluid and the vessel, thus increasing the collection rate, the process has several drawbacks. Firstly, placing multiple severed bones directly into small centrifuge tubes can cause them to move, squeeze, and break apart during centrifugation. This results in some bone marrow remaining in the gaps between bone fragments, or the pores being blocked by bone fragments, making some bone marrow difficult to collect. Secondly, bone marrow typically contains little fluid, and some viscous bone marrow adheres firmly to the inner wall of the medullary cavity, making complete centrifugation difficult. This results in some bone marrow remaining in the bone medullary cavity, affecting the collection rate. Furthermore, the adhering bone marrow, due to its low fluid content, is prone to inactivation. Therefore, after centrifugation, a pressurized device (such as a syringe or pipette) is usually used to promptly infuse the bone medullary cavity with liquid media (such as culture medium or PBS) to infiltrate and rinse away any remaining bone marrow, further improving the collection rate and maintaining bone marrow cell viability. However, since most of the bone marrow in the medullary cavity has already been centrifuged, the fluid resistance within the cavity decreases, requiring a large amount of liquid media to rinse away the adhering bone marrow.

[0004] Therefore, existing devices that use nested double centrifuge tubes to separate bone marrow have several drawbacks. The inner centrifuge tube is difficult to fix independently for multiple bones of different sizes, which can easily lead to multiple bones being squeezed and broken by centrifugation, affecting the collection rate. In addition, the device requires timely rinsing to separate the bone marrow adhering to the wall, which is difficult to centrifuge directly, resulting in a large consumption of liquid medium and increased operational difficulty. Utility Model Content

[0005] The purpose of this invention is to provide an adaptive and fixed bone marrow assembly separation device to solve the technical problem in the prior art that it is difficult to achieve rinsing and wetting of the liquid medium during centrifugation, resulting in insufficient bone marrow collection rate and reduced cell activity.

[0006] To solve the above-mentioned technical problems, this utility model specifically provides the following technical solution:

[0007] An adaptive fixation bone marrow assembly separation device includes a separation tube with a gradually decreasing inner bottom space. A core is assembled inside the separation tube, and the core has multiple uniformly distributed fixation channels for fixing the bone. After the outer wall of the core is in close contact with the inner wall of the separation tube, a liquid-carrying area is formed in the upper part of the separation tube, and the liquid-carrying area is used to fill the irrigation fluid.

[0008] In this process, after the core is inserted into the bottom of the separation tube, it is squeezed and deformed. At the same time, the fixing channel deforms synchronously and clamps the bone fixed inside it.

[0009] When the separation tube is sealed and centrifuged, the bone marrow in the fixed channel is separated by centrifugation. At the same time, the flushing fluid in the liquid-carrying area flushes the fixed bone marrow cavity in the fixed channel under centrifugation, thus realizing the integration of bone marrow centrifugation separation and bone marrow flushing.

[0010] As a preferred embodiment of this utility model, a plurality of wedge-shaped key blocks are provided in the inner bottom region of the separation tube, and the plurality of wedge-shaped key blocks are arranged circumferentially along the inner wall of the separation tube. A plurality of wedge-shaped keyways are provided on the bottom outer wall of the core, and the plurality of wedge-shaped keyways are arranged circumferentially along the outer wall of the core.

[0011] The inclination angle of the wedge-shaped keyway is smaller than that of the wedge-shaped key block, so that the core is not completely fitted into the separation tube in its natural state.

[0012] As a preferred embodiment of this utility model, the number of fixing channels is the same as the number of wedge-shaped keyways, and the fixing channels are located between adjacent wedge-shaped keyways. During centrifugation, the wedge-shaped keyways on the core are squeezed against the wedge-shaped key blocks on the separation tube under the action of centrifugal force, and the fixing channels are squeezed under the reaction force of the wedge-shaped key blocks. After deformation, the fixing channels become conical to strengthen the fixation of the skeleton.

[0013] As a preferred embodiment of this utility model, the wedge-shaped keyway has two inner triangular surfaces, and the two inner triangular surfaces are joined together on the side closest to the core axis to form an inner groove line. The distance between the inner groove line and the core axis gradually decreases from top to bottom.

[0014] The wedge-shaped key has two outer triangular faces, and the two outer triangular faces away from the axis of the separation tube are joined together to form an outer edge. The distance between the outer edge and the axis of the separation tube gradually decreases from top to bottom.

[0015] Wherein, the slope of the outer edge is greater than the slope of the inner groove, and the distance between the two inner triangular faces gradually increases from top to bottom, the distance between the two outer triangular faces gradually increases from top to bottom, and the increment of the distance between the two inner triangular faces from top to bottom is less than the increment of the distance between the two outer triangular faces from top to bottom.

[0016] As a preferred embodiment of this utility model, a collecting tube is fitted outside the separating tube, and a limiting structure is provided at the opening of the separating tube. The limiting structure maintains its relative position with the collecting tube under the action of centrifugal force.

[0017] The limiting structure is specifically a T-shaped ring whose outer edge of the separating tube protrudes outward and abuts against the opening of the collecting tube. Under the action of centrifugal force, the T-shaped ring is tightly attached to the collecting tube and restricts the relative movement between the separating tube and the collecting tube.

[0018] In a preferred embodiment of this utility model, the bottom of the separating tube converges towards the center and protrudes downward to form a nipple that communicates with the interior of the separating tube, and the nipple is coaxial with the collecting tube.

[0019] Compared with the prior art, this utility model has the following advantages:

[0020] This invention employs a pressurized assembly method to assemble the core at the bottom of a separation tube with gradually decreasing space. This causes the core to be squeezed and deformed, prompting the fixing channel to adaptively adjust the clamping force on the bone according to the centrifugal speed. A liquid-carrying area is formed above the core to fill the rinsing fluid and rinse the bone medullary cavity during centrifugation, thus integrating centrifugal separation and rinsing separation and improving the separation effect. Attached Figure Description

[0021] To more clearly illustrate the embodiments of this utility model or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0022] Figure 1 A schematic diagram of the structure of the adaptive fixation prefabricated bone marrow separation device is provided for embodiments of this utility model;

[0023] Figure 2A schematic diagram of the core portion structure of an adaptive fixation prefabricated bone marrow separation device provided for embodiments of this utility model;

[0024] Figure 3 A schematic diagram of the wedge-shaped key block and wedge-shaped keyway structure of the adaptive fixation bone marrow assembly separation device provided for embodiments of this utility model;

[0025] Figure 4 A schematic diagram of the fixation channel distribution of the adaptive fixation prefabricated bone marrow separation device is provided for embodiments of this utility model;

[0026] Figure 5 A schematic diagram of the collection tube portion of the adaptive fixation assembled bone marrow separation device provided for embodiments of this utility model.

[0027] The labels in the diagram represent the following:

[0028] 1-Separation tube; 2-Core; 3-Collection tube;

[0029] 11-Liquid-carrying area; 12-Wedge-shaped keyway; 13-Nipple; 21-Fixing channel; 22-Wedge-shaped keyway;

[0030] 121 - Outer triangular face; 122 - Outer edge; 221 - Inner triangular face; 222 - Inner groove. Detailed Implementation

[0031] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0032] like Figure 1 As shown, this utility model provides an adaptive fixation bone marrow assembly separation device, including a separation tube 1, the inner bottom space of the separation tube 1 gradually decreases, a core 2 is assembled in the separation tube 1, the core 2 has a plurality of uniformly distributed fixation channels 21 for fixing the bone, after the outer wall of the core 2 is in close contact with the inner wall of the separation tube 1, a liquid-carrying area 11 is formed in the upper part of the separation tube 1, and the liquid-carrying area 11 is used to fill the irrigation fluid.

[0033] In this process, after the core 2 is inserted into the bottom of the separation tube 1, it is squeezed and deformed. At the same time, the fixing channel 21 deforms synchronously and clamps the bone fixed inside it.

[0034] When the separation tube 1 is sealed and centrifuged, the bone marrow in the fixation channel 21 is separated by centrifugation. At the same time, the flushing fluid in the liquid-carrying area 11 flushes the fixed bone marrow cavity in the fixation channel 21 under centrifugation, thus realizing the integration of bone marrow centrifugation separation and bone marrow flushing.

[0035] The adaptive fixation bone marrow assembly separation device of this embodiment mainly utilizes the gradually decreasing space at the bottom of the separation tube 1 to assemble with the core 2. When the core 2 is assembled, it will be squeezed and deformed, causing the fixation channel 21 inside the core 2 to deform, thereby pressing the fixed bone inside and preventing it from falling off during centrifugation. In addition, the liquid-carrying area 11 formed after the core 2 and the separation tube 1 are tightly connected can be pre-filled with flushing fluid, so that during centrifugation, the flushing fluid can flow from the bone marrow cavity to flush the marrow cavity. That is, it integrates centrifugation separation and flushing separation, improving the effect of bone marrow separation.

[0036] Furthermore, during centrifugation, as the centrifugal speed increases, the centrifugal force on the core 2 increases, meaning the interaction force between the core 2 and the separation tube 1 becomes greater. This leads to increased deformation of the core 2 and increased internal force, causing the fixing channel 21 to more tightly compress the bone to improve the fixation effect. This achieves the function of adaptively adjusting the force on the fixed bone based on the centrifugal speed, thereby improving the bone fixation firmness and preventing its movement.

[0037] In this embodiment, the separation tube 1 can be fixed in any container for centrifugation. It is only necessary to ensure that the separation tube 1 remains fixed in the container, and the centrifugation is carried out by tilting centrifugation, that is, the container is placed in the centrifugation equipment at an angle, and the bottom of the separation tube 1 is away from the centrifugation axis.

[0038] Compared to conventional double-centrifuge-tube nested centrifugation separation devices, the adaptive fixation bone marrow assembly separation device of this embodiment uses a pressure assembly method to assemble the core 2 at the bottom of the separation tube 1 with gradually decreasing space. This causes the core 2 to be squeezed and deformed, prompting the fixation channel 21 to adaptively adjust the clamping force on the bone by adjusting the centrifugation speed. A liquid-carrying area 11 is formed above the core 2 to fill the rinsing fluid and rinse the bone marrow cavity during centrifugation, thus integrating centrifugation separation and rinsing separation and improving the separation effect.

[0039] The inner bottom space of the separation tube 1 gradually decreases, causing the core 2 to be squeezed and deformed after assembly. Of course, in order to further improve the effect of squeezing and deformation, the following preferred embodiment is provided.

[0040] like Figure 2 As shown, multiple wedge-shaped key blocks 12 are provided in the inner bottom region of the separation tube 1, and the multiple wedge-shaped key blocks 12 are arranged circumferentially along the inner wall of the separation tube 1. Multiple wedge-shaped keyways 22 are provided on the bottom outer wall of the core 2, and the multiple wedge-shaped keyways 22 are arranged circumferentially along the outer wall of the core 2.

[0041] The inclination angle of the wedge keyway 22 is smaller than that of the wedge key block 12, so that the core is not completely fitted into the separation tube 1 in its natural state.

[0042] In this embodiment, the wedge keyway 22 and the wedge key block 12 are partially fitted together, so that after the core 2 moves to the bottom of the separation tube 1 and is tightly fitted, the wedge key block 12 squeezes the wedge keyway 22, causing the core 2 to deform and transmit the deformation to the fixing channel 21, thereby achieving tight fixation for bones of different sizes.

[0043] Furthermore, since the wedge-shaped keyway 22 extends into the core 2, and the wedge-shaped key block 12 also engages within the wedge-shaped keyway 22, the transmission of deformation is further enhanced, enabling the fixing channel 21 to generate sufficient deformation after the core 2 is compressed. Of course, to further enhance the deformation of the fixing channel 21 after the core 2 is compressed, the following preferred embodiments are provided.

[0044] like Figure 4 As shown, the number of fixing channels 21 and wedge-shaped keyways 22 is the same, and the fixing channels 21 are located between adjacent wedge-shaped keyways 22. During centrifugation, the wedge-shaped keyways 22 on the core 2 are squeezed against the wedge-shaped key blocks 12 on the separation tube 1 under the action of centrifugal force, and the fixing channels 21 are squeezed under the reaction force of the wedge-shaped key blocks 12. After the fixing channels 21 are deformed, they become conical to strengthen the fixation of the bone.

[0045] In this embodiment, the fixing channel 21 is set between two adjacent wedge-shaped keyways 22. After the wedge-shaped keyway 22 is squeezed by the wedge-shaped key block 12, the elastic deformation is transmitted inward, which can squeeze the bone in the fixing channel 21 from two directions, thereby improving the effect of tight compression and fixation, and can further reduce the gap between the bone and the fixing channel 21 or reduce the cross-sectional area of ​​the gap.

[0046] The inner wall of the fixing channel 21 is also made of elastic material. Since the sizes of the bones to be separated vary, while the specifications of the fixing channel 21 remain constant, a core 2 with a diameter slightly larger than the largest bone diameter among the bones to be separated is used. Furthermore, during the material selection process, the size differences of the bones to be separated are minimized to effectively avoid significant variations in the tightness of the bone fixation. Since bones are typically not straight, even if the diameter of the fixing channel 21 is slightly larger than the bone diameter, when the bone is inserted into the fixing channel 21, it shifts within the channel and contacts the elastic wall of the fixing channel 21, generating significant resistance to movement. This ensures that the bone is unlikely to naturally dislodge within the fixing channel 21, facilitating assembly.

[0047] The deformation of the core 2 caused by the wedge-shaped keyway 22 being squeezed by the wedge-shaped key block 12 is due to the slope of the wedge-shaped key block 12 being greater than the slope of the wedge-shaped keyway 22, resulting in an incomplete assembly between the core 2 and the separation tube 1, that is, an incomplete fit between the wedge-shaped keyway 22 and the wedge-shaped key block 12. Therefore, in order to enable the deformation of the core 2 caused by the interaction force between the wedge-shaped keyway 22 and the wedge-shaped key block 12 to effectively fix the bone, the following preferred embodiment is provided.

[0048] like Figure 3 , Figure 4 As shown, the wedge-shaped keyway 22 has two inner triangular surfaces 221. The two inner triangular surfaces 221 are joined together on the side closest to the axis of the core 2 to form an inner groove line 222. The distance between the inner groove line 222 and the axis of the core 2 gradually decreases from top to bottom.

[0049] The wedge-shaped key block 12 has two outer triangular faces 121. The two outer triangular faces 121 are joined together on the side away from the axis of the separation tube 1 to form an outer edge 122. The distance between the outer edge 122 and the axis of the separation tube 1 gradually decreases from top to bottom.

[0050] Among them, the slope of the outer edge 122 is greater than the slope of the inner groove 222, and the distance between the two inner triangular faces 221 gradually increases from top to bottom, the distance between the two outer triangular faces 121 gradually increases from top to bottom, and the increase in the distance between the two inner triangular faces 221 from top to bottom is less than the increase in the distance between the two outer triangular faces 121 from top to bottom.

[0051] In this embodiment, by reducing the slope of the inner groove line 222 of the wedge keyway 22 and increasing the slope of the outer edge line 122 of the wedge key block 12, the compression area between the inner triangular surface 221 and the outer triangular surface 121 gradually increases when the wedge keyway 22 moves toward the wedge key block 12. This effectively increases the deformation area of ​​the fixing channel 21 during deformation transmission, meaning that the inner wall of the fixing channel 21 compresses the bone inward over a large area, further improving the fixation effect and the shrinkage effect.

[0052] Furthermore, by reducing the incremental distance between the two inner triangular surfaces 221 from top to bottom and increasing the incremental distance between the two outer triangular surfaces 121 from top to bottom, the compression deformation distance between the inner triangular surfaces 221 and the outer triangular surfaces 121 gradually increases as the wedge-shaped keyway 22 moves toward the wedge-shaped key block 12. This effectively enhances the deformation degree of the fixing channel 21 during deformation transmission, meaning that the inner wall of the fixing channel 21 significantly compresses the bone inward, further improving the fixation effect and the suture shrinkage effect.

[0053] To enable the separation tube 1 to be used in conventional test tube centrifugation equipment and to facilitate the collection of bone marrow fluid, the following preferred embodiments are provided.

[0054] like Figure 1 , Figure 2 , Figure 5 As shown, a collection tube 3 is fitted outside the separation tube 1. A limiting structure is provided at the opening of the separation tube 1. The limiting structure maintains its relative position with the collection tube 3 under the action of centrifugal force.

[0055] The limiting structure is specifically a T-shaped ring that protrudes outward from the outer edge of the separating tube 1 and abuts against the opening of the collecting tube 3. Under the action of centrifugal force, the T-shaped ring is tightly attached to the collecting tube 3 and restricts the relative movement between the separating tube 1 and the collecting tube 3.

[0056] Furthermore, the bottom of the separation tube 1 converges towards the center and protrudes downward to form a nipple 13 that connects to the interior of the separation tube 1. The nipple 13 is coaxial with the collection tube 3.

[0057] In this embodiment, by assembling the collection tube 3 outside the separation tube 1, and restricting the collection tube 3 and the separation tube 1 by the T-shaped ring, a collection area is formed at the bottom of the collection tube 3. Thus, during centrifugation, the centrifuged bone marrow and the bone marrow washed out by the rinsing fluid are mixed and enter the bottom of the collection tube 3 through the nipple 13.

[0058] The end face area of ​​the nipple 13 is minimized, that is, the wall thickness of the nipple 13 is reduced, thereby effectively reducing the contact area between the outflowing bone marrow fluid and the outside of the separation tube 1, which reduces the adhesion residue and increases the content of bone marrow cells in the collected fluid.

[0059] The above embodiments are merely exemplary embodiments of this application and are not intended to limit this application. The scope of protection of this application is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to this application within its substance and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of this application.

Claims

1. An adaptive, fixed, assembled bone marrow separation device, characterized in that, Includes a separation tube (1), the inner bottom space of the separation tube (1) gradually decreases, a core (2) is assembled inside the separation tube (1), the core (2) has a plurality of uniformly distributed fixation channels (21) for fixing the bone, after the outer wall of the core (2) is in close contact with the inner wall of the separation tube (1), a liquid-carrying area (11) is formed in the upper part of the separation tube (1), the liquid-carrying area (11) is used to fill the rinsing fluid; In this process, after the core (2) is inserted into the bottom of the separation tube (1), it is squeezed and deformed. At the same time, the fixing channel (21) deforms synchronously and clamps the bone fixed inside it. When the separation tube (1) is sealed and centrifuged, the bone marrow in the fixed channel (21) is separated by centrifugation. At the same time, the flushing fluid in the liquid-carrying area (11) flushes the fixed bone marrow cavity in the fixed channel (21) under centrifugation, thus realizing the integration of bone marrow centrifugation separation and bone marrow flushing.

2. The adaptive fixation bone marrow assembly separation device according to claim 1, characterized in that, Multiple wedge-shaped key blocks (12) are provided in the inner bottom region of the separation tube (1), and the multiple wedge-shaped key blocks (12) are arranged circumferentially along the inner wall of the separation tube (1). Multiple wedge-shaped keyways (22) are provided on the bottom outer wall of the core (2), and the multiple wedge-shaped keyways (22) are arranged circumferentially along the outer wall of the core (2). The inclination angle of the wedge keyway (22) is smaller than that of the wedge key block (12), so that the core is not completely fitted into the separation tube (1) in its natural state.

3. The adaptive fixation bone marrow assembly separation device according to claim 2, characterized in that, The number of the fixing channels (21) is the same as the number of the wedge-shaped keyways (22), and the fixing channels (21) are located between adjacent wedge-shaped keyways (22). During centrifugation, the wedge-shaped keyways (22) on the core (2) are squeezed against the wedge-shaped key blocks (12) on the separation tube (1) under the action of centrifugal force, and the fixing channels (21) are squeezed under the reaction force of the wedge-shaped key blocks (12). After the fixing channels (21) are deformed, they become conical to strengthen the fixation of the bones.

4. The adaptive fixation bone marrow assembly separation device according to claim 3, characterized in that, The wedge-shaped keyway (22) has two inner triangular faces (221). The two inner triangular faces (221) are joined together on the side closest to the axis of the core (2) to form an inner groove line (222). The distance between the inner groove line (222) and the axis of the core (2) gradually decreases from top to bottom. The wedge-shaped key block (12) has two outer triangular faces (121). The two outer triangular faces (121) are joined together on the side away from the axis of the separation tube (1) to form an outer edge (122). The distance between the outer edge (122) and the axis of the separation tube (1) gradually decreases from top to bottom. The slope of the outer edge (122) is greater than the slope of the inner groove (222), and the distance between the two inner triangular faces (221) gradually increases from top to bottom. The distance between the two outer triangular faces (121) gradually increases from top to bottom, and the increment of the distance between the two inner triangular faces (221) from top to bottom is less than the increment of the distance between the two outer triangular faces (121) from top to bottom.

5. The adaptive fixation bone marrow assembly separation device according to claim 1, characterized in that, A collection tube (3) is fitted outside the separation tube (1). A limiting structure is provided at the opening of the separation tube (1). The limiting structure maintains its relative position with the collection tube (3) under the action of centrifugal force. The limiting structure is specifically a T-shaped ring that protrudes outward from the outer edge of the separating tube (1) and abuts against the opening of the collecting tube (3). Under the action of centrifugal force, the T-shaped ring is tightly attached to the collecting tube (3) and restricts the relative movement between the separating tube (1) and the collecting tube (3).

6. The adaptive fixation bone marrow assembly separation device according to claim 5, characterized in that, The bottom of the separation tube (1) converges towards the center and protrudes downward to form a nipple (13) that connects to the inside of the separation tube (1). The nipple (13) is coaxial with the collection tube (3).

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