Speed-adjustable protein stirring and homogenizing device

Abstract

The utility model relates to a protein stirring homogenizing equipment of adjustable rotating speed belongs to protein processing instrument technical field, this protein stirring homogenizing equipment of adjustable rotating speed, include: work table and water bath bearing mechanism, the top fixed coupling of work table has the lifter stirrer, the surface fixed coupling of lifter stirrer has motor speed regulator controller, the water bath bearing mechanism includes the water bath bucket of placing in work table top, the inside embedding of water bath bucket installs has refrigerator, the vertical inner wall fixed coupling of water bath bucket has first liquid guide pipe, water bath bearing mechanism carries out refrigeration to cooling liquid through the refrigerator in water bath bucket, and realizes the circulating flow of cooling liquid through first liquid guide pipe, liquid delivery pump and second liquid guide pipe, thereby efficiently takes away the heat generated in homogenizing process, maintains low temperature environment, prevents protein denaturation, ensures the reliability of experimental result.

Description

Technical Field

[0001] This utility model relates to the field of protein processing equipment technology, and in particular to a protein stirring and homogenizing device with adjustable rotation speed. Background Technology

[0002] Protein homogenizers are key devices for disrupting biological samples to release proteins, processing tissue, cell, or microbial samples through mechanical shearing forces. Motorized homogenizers with adjustable rotation speeds are currently the most common type, offering a variety of efficient solutions for protein extraction.

[0003] The friction between the high-speed rotating blade and the sample generates a large amount of heat, which can easily lead to the denaturation and inactivation of temperature-sensitive proteins. This problem can seriously affect the accuracy and reliability of subsequent experimental results. Utility Model Content

[0004] Therefore, it is necessary to provide a protein mixing and homogenizing device with adjustable speed to address the problem that existing protein homogenizers generate heat during operation, which causes denaturation of the target protein and affects the reliability of subsequent experimental results.

[0005] An adjustable-speed protein mixing and homogenizing device includes: a worktable and a water bath support mechanism, wherein a lifting stirrer is fixedly connected to the top of the worktable, and a motor speed controller is fixedly connected to the surface of the lifting stirrer.

[0006] In one embodiment, the water bath support mechanism includes a water bath placed on top of the workbench, a cooler is embedded inside the water bath, a first liquid guide tube is fixedly connected to the vertical inner wall of the water bath, an infusion pump is fixedly connected and communicated to the surface of the first liquid guide tube, a second liquid guide tube is fixedly connected and communicated to the outlet end of the infusion pump, one end of the second liquid guide tube extends into the interior of the water bath, and the second liquid guide tube is positioned above the first liquid guide tube.

[0007] In one embodiment, one end of the second liquid guide tube is fixedly connected to and communicates with an annular pipe that is fixedly connected to the water bath, and the lower surface of the annular pipe is fixedly connected to and communicates with nozzles distributed in a ring.

[0008] In one embodiment, the number of nozzles is not less than ten, and the nozzles are arranged in a ring around the axis of the water bath.

[0009] In one embodiment, the opening of the nozzle intersects the axis of the water bath.

[0010] In one embodiment, a valve is fixedly connected to and communicates with the surface of the water bath, and the lowest point of the connection between the valve and the water bath is flush with the inner bottom wall of the water bath.

[0011] In one embodiment, the bottom wall of the water bath is sloping, and the lowest point of the bottom wall of the water bath is aligned with the lowest point of the connection between the valve and the water bath.

[0012] In one embodiment, a sealing ring is embedded inside the opening of the water bath tub, and the sealing ring is a silicone material component.

[0013] In one embodiment, the top of the water bath has evenly distributed adjustment grooves with rounded corners.

[0014] Beneficial effects 1. In the above-mentioned adjustable-speed protein mixing and homogenizing equipment, the water bath support mechanism cools the coolant through the coolant in the water bath tank, and realizes the circulation of the coolant through the first liquid guide pipe, the liquid pump and the second liquid guide pipe, thereby efficiently removing the heat generated during homogenization, maintaining a low-temperature environment, preventing protein denaturation and ensuring the reliability of experimental results.

[0015] 2. The lifting stirrer achieves stepless speed adjustment through a motor speed controller. Users can flexibly control the mechanical shear force according to the characteristics of the protein sample, reduce frictional heat generation, and thus reduce the risk of protein denaturation. Combined with a water bath cooling system, it further improves the accuracy and repeatability of experiments. Attached Figure Description

[0016] To more clearly illustrate the technical solutions in this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are some embodiments of this utility model. For those skilled in the art, other drawings can be obtained from these drawings without creative effort.

[0017] Figure 1 This is a schematic diagram of the overall structure of this utility model; Figure 2 This is a schematic diagram of the overall structure of the water bath support mechanism in this utility model; Figure 3 for Figure 2 Schematic sectional view along the middle AA direction; Figure 4 for Figure 2 Cross-sectional view along the middle BB direction; Figure 5 This is a partial structural schematic diagram of the water bath support mechanism in this utility model.

[0018] Figure label: 100. Workbench; 200. Lifting agitator; 300. Motor speed controller; 400. Water bath support mechanism; 410. Water bath tank; 411. Adjustment tank; 420. Refrigerator; 430. First liquid guide pipe; 440. Infusion pump; 450. Second liquid guide pipe; 460. Annular pipeline; 470. Nozzle; 480. Valve; 490. Sealing ring. Detailed Implementation

[0019] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. All other embodiments obtained by those skilled in the art based on the embodiments of this utility model without creative effort are within the scope of protection of this utility model.

[0020] The following is combined with Figure 1 - Figure 5 This invention describes an adjustable-speed protein mixing and homogenizing device.

[0021] In one embodiment, an adjustable-speed protein mixing and homogenizing device includes: a workbench 100 and a water bath support mechanism 400, with a lifting mixer 200 fixedly connected to the top of the workbench 100 and a motor speed controller 300 fixedly connected to the surface of the lifting mixer 200. Among them, the lifting agitator 200 is the core power and execution component of the equipment, and its specific structure is as follows: The lifting agitator 200 includes a support column fixedly connected to the top of the worktable 100. The support column has a precision lifting slide rail, along which a lifting slider driven by a lifting drive motor (such as a stepper motor) via a synchronous belt or ball screw can move vertically. A mounting plate is located at the front end of this lifting slider, and a stirring motor, serving as the core power source, is fixed to this mounting plate. The power output shaft of the stirring motor is connected to a long stirring drive shaft via a coupling, transmitting torque to the stirring blades mounted at the end. The stirring motor is electrically connected to a motor speed controller 300 via a cable, receiving speed control signals from it. A height gauge is typically provided next to the support column for precise control of the immersion depth. The lifting slider is equipped with a manual locking knob, which can be used to fix the position after adjustment to prevent slippage during stirring.

[0022] The procedure for adjusting the speed of protein mixing in the inner container of the water bath 410 using a lifting stirrer 200 is as follows: Preparation: Place the container containing the protein sample inside the water bath 410, inject coolant into the water bath 410, turn on the cooler 420 embedded inside the water bath 410, and pre-cool the water bath to the target temperature (e.g., 4°C). Loosen the manual locking knob on the lifting slider, and manually raise the entire stirring component (including the stirring motor, stirring drive shaft, and stirring blades) to a suitable height. After confirming with the height gauge that the stirring blades are completely submerged below the liquid surface and do not touch the bottom of the container, tighten the manual locking knob to lock the position. Install the selected stirring blades onto the end of the stirring drive shaft and secure them. Slightly adjust the position of the container to ensure that the stirring drive shaft is on the central axis of the container.

[0023] Speed ​​Setting: Manually turn on the power to the motor speed controller 300 and set the desired initial speed using the control unit (knob or button) on the motor speed controller 300. For denatured protein samples, start with a low speed. Press the start button on the motor speed controller 300, and the stirring motor will start running at the set speed, driving the stirring blades to homogenize the protein solution. During the stirring process, the speed of the stirring motor can be continuously adjusted via the motor speed controller 300 to adapt to different mixing needs. To improve homogenization efficiency, increase the speed slowly; if excessive foam or heat is generated, decrease the speed promptly.

[0024] End of operation: After stirring is complete, first slowly reduce the speed to zero using the motor speed controller 300, then turn off the stirring motor, loosen the manual locking knob, raise the lifting slider of the lifting stirrer 200 so that the stirring blades are completely removed from the liquid surface, remove the sample container, then turn off the power to the cooler 420 and the infusion pump 440, clean the stirring blades and the water bath 410, and prepare for the next use.

[0025] like Figure 2 , Figure 3 , Figure 4 and Figure 5As shown, the water bath support mechanism 400 includes a water bath 410 placed on top of the workbench 100. A cooler 420 is embedded inside the water bath 410. A first liquid guide pipe 430 is fixedly connected to the vertical inner wall of the water bath 410. A liquid pump 440 is fixedly connected and connected to the surface of the first liquid guide pipe 430. A second liquid guide pipe 450 is fixedly connected and connected to the outlet end of the liquid pump 440. One end of the second liquid guide pipe 450 extends into the interior of the water bath 410. The second liquid guide pipe 450 is positioned above the first liquid guide pipe 430. The cooler 420 can be a semiconductor cooler as needed. This device uses the Peltier effect to achieve precise temperature control. Its core structure includes an alumina ceramic substrate, PN semiconductor couples (such as TEC1-12706 containing 127 pairs of couples), and copper guide plates. After being energized, a cold end face (heat absorption) and a hot end face (heat release) are formed. It needs to be used with aluminum heat sink fins and a fan for forced heat dissipation. In terms of working principle, direct current drives the charge carrier to transfer heat, pumping the heat of the coolant inside the water bath 410 to the outside, thus achieving continuous cooling. The typical model TEC1-12706 (40×40×3.6mm) is rated at 12V / 6A, with a maximum temperature difference of 67℃. It is suitable for multi-panel assembly in laboratory equipment to meet the low-temperature requirements of protein mixing and homogenization.

[0026] like Figure 2 , Figure 3 , Figure 4 and Figure 5 As shown, the water bath support mechanism 400 delivers coolant to the annular pipe 460, which is fixedly connected to the water bath 410, via the second liquid guide pipe 450, achieving uniform fluid distribution. The lower surface of the annular pipe 460 is fixedly connected to and connected to a ring of nozzles 470, with no fewer than ten nozzles 470 arranged in a ring around the axis of the water bath 410, forming a uniform, all-around spray coverage without dead zones, ensuring consistent heat exchange across all parts of the sample container. The openings of the nozzles 470 intersect the axis of the water bath 410, generating tangential momentum in the sprayed coolant flow, enhancing fluid turbulence and heat exchange efficiency within the water bath. A valve 480 is fixedly connected to and connected to the surface of the water bath 410. The lowest point of the valve 480's connection to the water bath 410 is flush with the inner bottom wall of the water bath 410, enabling complete emptying of the liquid from the container. The inner bottom wall of the water bath 410 is sloped, and the lowest point of the inner bottom wall of the water bath 410 is aligned with the lowest point of the connection between the valve 480 and the water bath 410, ensuring that the liquid can be completely drained by gravity without residue, facilitating maintenance and cleaning. A sealing ring 490 is embedded inside the opening of the water bath 410. The sealing ring 490 is made of silicone material, providing excellent sealing performance to prevent coolant leakage and external contamination. The top of the water bath 410 has evenly distributed adjustment grooves 411 with rounded corners, providing flexible passage space for containers and pipelines of different sizes while avoiding stress concentration.

[0027] Working Principle: When the material requires a low-temperature environment, a measured amount of coolant needs to be added to the water bath 410 before placing it in the container. The built-in cooler 420 in the water bath 410 first lowers the coolant to the target low temperature, establishing a stable cold source. After stirring begins, the pump 440 draws the heated coolant through the first liquid guide pipe 430 and pumps it into the upper annular pipe 460 through the second liquid guide pipe 450. Finally, it is evenly sprayed downwards through the annularly distributed nozzles 470, forming a highly efficient shower system. This actively and evenly removes heat, significantly reducing the probability of protein denaturation. After the heat exchange is complete, the liquid returns and collects at the unique sloping inner bottom of the water bath 410, finally being completely emptied through the open valve 480 at the lowest point. This achieves efficient replacement of the cooling medium, ensuring ease of operation and repeatability of the experiment.

[0028] It should be noted that the lifting drive motor, stirring motor, motor speed controller 300, cooler 420 and infusion pump 440 mentioned above are all devices with relatively mature existing technology. The specific models can be selected according to actual needs. At the same time, the lifting drive motor, stirring motor, motor speed controller 300, cooler 420 and infusion pump 440 are all powered by AC mains power. The specific power supply method is selected according to the situation and will not be elaborated here.

[0029] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A speed-adjustable protein homogenizing device, characterized by, include: A workbench (100) is provided with a lifting mixer (200) fixedly connected to the top of the workbench (100), and a motor speed controller (300) is fixedly connected to the surface of the lifting mixer (200). A water bath support mechanism (400) includes a water bath (410) placed on top of a workbench (100). A cooler (420) is embedded inside the water bath (410). A first liquid guide pipe (430) is fixedly connected to the vertical inner wall of the water bath (410). An infusion pump (440) is fixedly connected and communicated to the surface of the first liquid guide pipe (430). A second liquid guide pipe (450) is fixedly connected and communicated to the outlet end of the infusion pump (440). One end of the second liquid guide pipe (450) extends into the interior of the water bath (410). The second liquid guide pipe (450) is positioned above the first liquid guide pipe (430).

2. The adjustable speed protein blending homogenization apparatus of claim 1, wherein, One end of the second liquid guide tube (450) is fixedly connected to and communicates with an annular pipe (460) which is fixedly connected to the water bath (410). The lower surface of the annular pipe (460) is fixedly connected to and communicates with nozzles (470) distributed in a ring.

3. The adjustable speed protein blending homogenization apparatus of claim 2, wherein, The number of nozzles (470) is not less than ten, and the nozzles (470) are arranged in a ring around the axis of the water bath (410).

4. The adjustable speed protein blending homogenization apparatus of claim 2, wherein, The opening of the nozzle (470) intersects the axis of the water bath (410).

5. The adjustable speed protein blending homogenization apparatus of claim 1, wherein, The surface of the water bath (410) is fixedly connected to and connected to a valve (480), and the lowest point of the connection between the valve (480) and the water bath (410) is flush with the inner bottom wall of the water bath (410).

6. The adjustable speed protein blending homogenization apparatus of claim 5, wherein, The bottom wall of the water bath (410) is sloping, and the lowest point of the bottom wall of the water bath (410) is aligned with the lowest point of the connection between the valve (480) and the water bath (410).

7. The adjustable speed protein blending homogenization apparatus of claim 1, wherein, A sealing ring (490) is embedded in the inner side of the opening of the water bath (410), and the sealing ring (490) is a silicone material component.

8. The adjustable speed protein blending homogenization apparatus of claim 1, wherein, The top of the water bath (410) is provided with evenly distributed adjustment grooves (411), and the adjustment grooves (411) are rounded.

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