Two-dimensional separation and imaging technology for the rapid analysis of biological samples
The method provides a rapid and accurate two-dimensional separation of complex biological samples on a non-porous metallic surface, enhancing MALDI analysis by using mechanical, hydrodynamic, and aerodynamic means, addressing the inefficiencies of conventional chromatographic methods.
DE102015122102B4Active Publication Date: 2025-11-27MICROMASS UK LTD
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Patent Information
- Application Number
- DE102015122102
- Authority / Receiving Office
- DE · DE
- Patent Type
- Patents
- Current Assignee / Owner
- Priority Date
- 2014-12-17
- Filing Date
- 2015-12-17
- Publication Date
- 2025-11-27
- Estimated Expiration
- 2035-12-17
AI Technical Summary
Technical Problem
Conventional chromatographic separation methods for complex biological samples are time-consuming and not ideally suited for subsequent MALDI analysis, leading to broadening of mass spectrum peaks and deterioration of mass accuracy.
Method used
A method for two-dimensional separation of sample components on a non-porous metallic target surface using mechanical, hydrodynamic, and/or aerodynamic means, followed by ionization and mass analysis suitable for MALDI.
Benefits of technology
Enables rapid, simple, and accurate MALDI analysis with improved mass accuracy and suitability for imaging, eliminating the need for complex procedures and optimizing the surface for subsequent MALDI analysis.
✦ Generated by Eureka AI based on patent content.
Abstract
A method for ion mapping of a sample, which has the following features: Application of a liquid sample to a metallic target surface, Separation of components of the liquid sample on the metallic target surface according to one or more physicochemical properties in a first spatial dimension and in a second spatial dimension and Ionizing and mass-analyzing multiple separated areas of the separated sample to generate an ion map of at least a portion of the sample separated on the target surface, wherein the components of the sample are applied to the target surface and separated by mechanical, hydrodynamic and / or aerodynamic means, and wherein the one or more physicochemical properties include one or more from the group comprising surface binding, surface affinity, solubility, binding affinity and polarity.
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