Evaluation method, method for selecting scalp and hair cosmetics, screening method, method for manufacturing scalp and hair cosmetics, scalp and hair cosmetics, and evaluation support tool

Abstract

To provide a novel evaluation method related to the barrier function of the scalp, to provide a method for selecting scalp and hair cosmetics, to provide a screening method, to provide a method for producing scalp and hair cosmetics, to provide scalp and hair cosmetics, and to provide an evaluation support tool.SOLUTION: The degree of the barrier function in the scalp of a subject to be evaluated is evaluated based on at least one of the following indicators: an indicator of bacterial diversity in the scalp bacterial flora of a subject to be evaluated; an indicator of the number of bacteria in the scalp bacterial flora of a subject to be evaluated that includes at least Corynebacterium bacteria; and an indicator of the proportion of bacteria in the scalp bacterial flora of a subject to be evaluated that includes at least Corynebacterium bacteria. Or, at least any one of the above indicators is evaluated based on the degree of the barrier function in the scalp of a subject to be evaluated.SELECTED DRAWING: Figure 3

Description

Technical Field

[0001] The present invention relates to an evaluation method, a method for selecting a scalp and hair cosmetic, a screening method, a method for manufacturing a scalp and hair cosmetic, a scalp and hair cosmetic, and an evaluation support tool.

Background Art

[0002] It is known that the scalp can be dried by various causes such as excessive shampooing, heat from a dryer, and ultraviolet rays, and its barrier function can decline. And in the scalp with a declined barrier function, scalp itching and dandruff are likely to occur. Therefore, for example, in Patent Document 1 (Japanese Patent Application Laid-Open No. 2015-155395), a scalp and hair cosmetic containing a component for improving the barrier function of the scalp has been proposed.

Prior Art Documents

Patent Documents

[0003]

Patent Document 1

Summary of the Invention

Problems to be Solved by the Invention

[0004] However, in order to grasp whether improvement of the barrier function is required for the state of the scalp, conventionally, it has remained at reports such as measurement of the trans epidermal water loss (TEWL) of the scalp and observation of the degree of stratum corneum peeling by tape stripping of the scalp. For this reason, a new objective evaluation method related to the barrier function of the scalp has been demanded.

[0005] In view of the above circumstances, an object of the present invention is to provide a new evaluation method related to the barrier function of the scalp, a method for selecting a scalp and hair cosmetic, a screening method, a method for manufacturing a scalp and hair cosmetic, a scalp and hair cosmetic, and an evaluation support tool.

Means for Solving the Problems

[0006] In order to find a novel evaluation method related to the barrier function of the scalp, the present inventor focused on the scalp microbiota and conducted intensive research on the relationship between the barrier function of the scalp and the scalp microbiota.

[0007] As a result of detailed examination of the relationship between the bacteria in the scalp microbiota and the barrier function of the scalp, the present inventor newly found that there is a correlation between the diversity of bacteria in the scalp microbiota of the subject to be evaluated, the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject to be evaluated, the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject to be evaluated, and the barrier function of the scalp. Focusing on using this relationship as an evaluation method for the barrier function of the scalp, the present invention has been completed.

[0008] That is, the evaluation method of the present invention comprises the following means. [1] Based on at least any one of an index of the diversity of bacteria in the scalp microbiota of the subject to be evaluated, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject to be evaluated, and an index related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject to be evaluated, evaluate the degree of the barrier function of the scalp of the subject to be evaluated, or, based on the degree of the barrier function of the scalp of the subject to be evaluated, evaluate at least any one of an index of the diversity of bacteria in the scalp microbiota of the subject to be evaluated, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject to be evaluated, and an index related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject to be evaluated.

[0009] [2] The index of bacterial diversity is at least one of the number of bacterial species present on the scalp and the evenness of the proportions of each bacterial species present on the scalp. The index related to the number of bacteria is at least one of the number of bacteria of the genus Corynebacterium in the scalp microbiota of the subject, the total number of bacteria of acne bacteria, Corynebacterium bacteria, and Staphylococcus epidermidis, and the total number of bacteria. The index related to the proportion of bacteria is the proportion of bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and is the evaluation method described in the previous item [1].

[0010] [3] The degree of the barrier function of the subject is evaluated by comparing the subject's index with the comparison standard of the index, or the subject's index is evaluated by comparing the degree of the subject's barrier function with the comparison standard of the degree of the barrier function, which is the evaluation method described in the previous item [1] or [2].

[0011] The method for selecting a scalp and hair cosmetic of the present invention comprises the following means. [4] A method for selecting a scalp and hair cosmetic, which comprises selecting a scalp and hair cosmetic to be recommended to the subject according to the evaluation result obtained by the evaluation method according to any one of items [1] to [3] above.

[0012] The screening method of the present invention comprises the following means. [5] A screening method for a scalp and hair cosmetic for improving the barrier function of the scalp, which comprises a step of treating the scalp and / or hair of the subject with a scalp and hair cosmetic at least once, and comparing at least one of the index of bacterial diversity in the scalp microbiota of the subject before and after the step of treating the scalp and / or hair, the index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and the index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject.

[0013] The screening method of the present invention comprises the following means. [6] A step of treating the scalp and / or hair of an evaluated person with a candidate substance for an active ingredient at least once, an index of the diversity of bacteria in the scalp microbiota of the evaluated person before and after the step of treating the scalp and / or hair, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the evaluated person, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the evaluated person, and a step of comparing at least any one of the indices. A screening method for a candidate substance for an active ingredient for improving the barrier function of the scalp.

[0014] The method for producing a scalp and hair cosmetic of the present invention comprises the following means. [7] Blending an active ingredient selected by the screening method described in the previous item [6]. A method for producing a scalp and hair cosmetic.

[0015] The scalp and hair cosmetic of the present invention comprises the following means. [8] A scalp and hair cosmetic containing an active ingredient selected by the screening method described in the previous item [5] or an active ingredient selected by the screening method described in the previous item [6].

[0016] The evaluation support tool of the present invention comprises the following means. [9] An evaluation support tool for supporting the evaluation of the degree of the barrier function of the scalp of an evaluated person, an acquisition unit for acquiring index identification information for identifying at least any one of an index of the diversity of bacteria in the scalp microbiota of the evaluated person, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the evaluated person, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the evaluated person, a storage unit storing information serving as a comparison criterion for the index, a comparison unit for comparing the index of the evaluated person specified from the index identification information acquired by the acquisition unit with the comparison criterion of the index stored in the storage unit, and a display unit for displaying and outputting the comparison result by the comparison unit. An evaluation support tool.

[0017] The evaluation support tool of the present invention comprises the following means.

[10] An evaluation support tool for supporting the evaluation of at least any one of the indices related to the diversity of bacteria in the scalp microbiota of the subject, the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, the evaluation support tool comprising: an acquisition unit that acquires barrier function specific information for specifying the degree of the barrier function of the subject's scalp; a storage unit that stores information serving as a comparison criterion for the degree of the barrier function of the scalp; a comparison unit that compares the degree of the barrier function of the subject's scalp specified from the barrier function specific information acquired by the acquisition unit with the comparison criterion for the degree of the barrier function of the scalp stored in the storage unit; and a display unit that displays and outputs the comparison result by the comparison unit.

Effects of the Invention

[0018] According to the evaluation method of the present invention, a novel evaluation method related to the barrier function of the scalp is provided. According to the selection method of the present invention, it is possible to select a scalp and hair cosmetic suitable for the subject. According to the screening method of the present invention, it becomes easy to select a component advantageous for improving the barrier function of the subject's scalp. According to the production method of the present invention, a scalp and hair cosmetic capable of improving the barrier function of the subject's scalp can be obtained. According to the scalp and hair cosmetic of the present invention, it becomes possible to improve the barrier function of the subject's scalp. According to the evaluation support tool of the present invention, the above novel evaluation method is supported.

Brief Description of the Drawings

[0019]

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Mode for Carrying Out the Invention

[0020] Hereinafter, an evaluation method, a method for selecting a scalp and hair cosmetic, a screening method, a method for manufacturing a scalp and hair cosmetic, and an evaluation support tool according to each embodiment of the present invention will be described by way of example, but these do not particularly limit the invention. In the present invention, the term "and / or" refers to both or either one.

[0021] (1) Evaluation method (1-1) Evaluation method of the first embodiment The evaluation method of the first embodiment is a method for evaluating the degree of the barrier function of the scalp of an evaluated person based on at least any one of an index of the diversity of bacteria in the scalp flora of the evaluated person, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person, and an index related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person. According to the evaluation method, it becomes possible to objectively evaluate the degree of the barrier function of the scalp of the evaluated person. Note that the evaluation method can be used for cosmetic purposes and excludes medical acts performed by doctors and medical staff.

[0022] (Barrier function of the scalp) The barrier function of the scalp generally refers to a function of preventing the loss of moisture and biological components existing inside the scalp, or a function of preventing foreign substances (microorganisms, viruses, dust, etc.) from entering from the outside of the scalp into the living body. The barrier function is not particularly limited, but can be grasped, for example, by examining the trans-epidermal water loss (TEWL). In this case, the higher the value of TEWL (TEWL score) of the scalp, the more moisture is evaporated through the stratum corneum, indicating that the barrier function of the scalp is low. This trans-epidermal water loss (TEWL) can be measured using, for example, a known closed or open type water evaporimeter.

[0023] In addition to the above-mentioned trans-epidermal water loss, the barrier function can be grasped, for example, by examining the degree to which multiple exfoliations of scalp keratinocytes occur due to tape stripping. In this case, the greater the degree of multiple exfoliations of keratinocytes due to tape stripping on the scalp, the more likely it is that keratinocyte exfoliation will occur, indicating a lower scalp barrier function. As for the degree of multiple exfoliations, for example, the greater the number of multiple exfoliations per unit area and the greater the multiplicity (the number of overlapping keratinocytes), the more severe the degree of multiple exfoliations is judged to be. The degree of multiple exfoliations of these keratinocytes can be grasped, for example, by observing the tape-stripped sample under a microscope. It should be noted that the keratinocytes exfoliated by this tape stripping only exfoliate already dead cells, not living cells.

[0024] (Index regarding bacteria in scalp microbiota) The degree of the scalp barrier function can be evaluated by any one of the index of the diversity of bacteria in the scalp microbiota of the subject, the index regarding the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and the index regarding the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, or a combination of these indexes. When using the indexes obtained by combination, it is preferable to use indexes obtained by combining those with a strong correlation with the degree of the scalp barrier function from the viewpoint of enhancing the reliability of the evaluation.

[0025] The scalp microbiota means a collection of a group of bacteria present on the scalp. The scalp microbiota can be analyzed, for example, by the 16S rRNA sequencing method using a next-generation DNA sequencer with a specimen collected from a partial area of the scalp to be the evaluation target site by an appropriate means such as an adhesive tape.

[0026] (Index of the diversity of bacteria in scalp microbiota) As an index of the diversity of bacteria in the scalp microbiota, for example, the number of types of bacteria present on the scalp or the evenness of the ratio of each type of bacteria present on the scalp can be used. Here, as an index of the diversity of bacteria in the scalp microbiota, it may be evaluated that the higher the number of types of bacteria present on the scalp or the higher the evenness of the ratio of each type of bacteria present on the scalp, the higher the diversity of bacteria in the scalp microbiota. In the evaluation using this index, it can be evaluated that the higher the barrier function of the scalp, the higher the diversity of bacteria in the scalp microbiota can be evaluated by this index.

[0027] Note that "the evenness of the ratio of each type of bacteria present on the scalp is high" means that the ratios of each type of bacteria whose presence is confirmed on the scalp are approximately the same, and although not particularly limited, it may be represented by, for example, the standard deviation. Further, as an index of diversity, for example, an index obtained by attaching more weight to the evenness of the ratio for each type than the number of types of bacteria, or an index obtained by attaching more weight to the number of types of bacteria than the evenness of the ratio for each type may be used.

[0028] As the above index of diversity, for example, indices such as the Shannon index, Simpson diversity index, Pielou's evenness index, Chao1 index, Ace index, and dominant species occupancy rate may be used. Therefore, in the evaluation method of the first embodiment, as an index of the diversity of bacteria in the scalp microbiota, any one of the Shannon index, Simpson diversity index, Pielou's evenness index, Chao1 index, Ace index, and dominant species occupancy rate can be used.

[0029] As the above index of diversity, preferably, the Shannon index can be used. The Shannon index is an index using the Shannon-Wiener diversity index as an index indicating the diversity of bacteria in the scalp microbiota, and can be calculated based on the types of bacteria and their respective occupancy rates (ratios) in the scalp microbiota. The Shannon index is calculated by the following formula for Shannon Index H'.

[0030]

Number

[0031] In the above formula, "S" represents the number of types of bacteria observed in the sample. "Pi" represents the proportion of bacteria i in the entire sample. Note that the types of bacteria are specified such that the sum of the proportions of each bacteria in the sample is "1". For example, it is an index with a higher value when the number of types of bacteria is large and each type of bacteria exists in an equal proportion. Note that in the above formula, by taking the logarithm, weight is given to rare types of bacteria.

[0032] Note that the identification of the types of bacteria in the diversity index can be performed according to the "species" in the scalp microbiota. For bacteria for which the "species" is not identified, the types can be identified using the "genus", which is a higher taxonomic rank, as a unit. Similarly, for bacteria for which the "genus" is not identified, the types can be identified using the "family", which is a higher taxonomic rank, as a unit.

[0033] As a method for identifying the types of bacteria in the scalp microbiota, for example, using a specimen collected from a partial area of the scalp as the evaluation target site by an appropriate means such as an adhesive tape, and analyzing the analysis results of the scalp microbiota by the 16S rRNA sequencing method using a next-generation DNA sequencer with a pipeline analysis tool at the genus level or species level of bacteria, it can be identified. In the above 16S rRNA sequencing method, by appropriately using commercially available primers, the types of bacteria in the diversity index can be identified in units of species, genus, or family. Note that as the above primers, for example, commercially available primers that specifically recognize one or more regions selected from the V1, V2, V3, and V4 regions of the 16S rRNA of bacteria (for example, 341f-805r, 27Fmod-338R manufactured by Biotechnology Research Co., Ltd.) can be used.

[0034] (Index related to the number of bacteria including bacteria of the genus Corynebacterium in the scalp flora) As an index related to the number of bacteria including bacteria of the genus Corynebacterium in the scalp flora, for example, it may be an index in which the larger the number of bacteria of the genus Corynebacterium in the scalp flora, the larger the value. In the evaluation using this index, the higher the value of this index, the lower the barrier function of the scalp can be evaluated.

[0035] The above-mentioned "bacteria of the genus Corynebacterium in the scalp flora" refers to bacteria classified into the genus Corynebacterium that exist on the scalp. Bacteria classified into the genus Corynebacterium can be identified, for example, by analysis at the genus level using the 16S rRNA sequencing method described above.

[0036] As an index related to the number of bacteria including bacteria of the genus Corynebacterium in the scalp flora, for example, any of the following indices may be used. · The number of bacteria of the genus Corynebacterium in the scalp flora · An index obtained by using the number of bacteria of the genus Corynebacterium in the scalp flora and the number of other bacteria in the scalp flora, and the number of bacteria of the genus Corynebacterium is weighted more than the number of other bacteria · The total number of bacteria of Cutibacterium acnes, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora · The number of bacteria obtained by adding the number of bacteria that exist in the scalp flora at a predetermined ratio or more to the total number of bacteria of Cutibacterium acnes, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora · The total number of bacteria in the scalp flora (the number of all bacteria in the scalp flora) · An index obtained by appropriately combining these indices

[0037] As an index regarding the number of bacteria including bacteria of the genus Corynebacterium in the scalp flora, from the viewpoint of enhancing the reliability of the evaluation of the degree of the scalp barrier function, the number of bacteria of the genus Corynebacterium in the scalp flora, the total number of bacteria of Propionibacterium acne, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora, and the total number of bacteria in the scalp flora, it is preferable to use at least any one of them as an index, and it is more preferable to use the number of bacteria of the genus Corynebacterium in the scalp flora or the total number of bacteria of Propionibacterium acne, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora as an index, and it is even more preferable to use the number of bacteria of the genus Corynebacterium in the scalp flora as an index.

[0038] In the scalp flora, the number of bacteria of Propionibacterium acne, the number of bacteria of the genus Corynebacterium, and the number of bacteria of Staphylococcus epidermidis can be analyzed, for example, by analyzing the results of the analysis of the scalp flora by the above 16S rRNA sequencing method at the genus level or species level of bacteria using a pipeline analysis tool. Also, the total number of bacteria of Propionibacterium acne, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis, and further, the total number of all bacteria in the scalp flora including bacteria other than these bacteria (total number of bacteria) can be grasped in the same way. Note that the number of bacteria of Propionibacterium acne, the number of bacteria of the genus Corynebacterium, and the number of bacteria of Staphylococcus epidermidis can also be obtained by a method (for example, quantitative PCR method) for measuring the copy number (DNA amount) of each bacterium in the scalp flora in addition to the above 16S rRNA sequencing method.

[0039] (Index regarding the ratio of bacteria including bacteria of the genus Corynebacterium in the scalp flora) As an index regarding the ratio of bacteria including bacteria of the genus Corynebacterium in the scalp flora, for example, it may be an index such that the larger the ratio of bacteria including bacteria of the genus Corynebacterium in the scalp flora, the larger the value. In the evaluation using the index, it can be evaluated that the lower the scalp barrier function, the larger the index.

[0040] As an index regarding the ratio of bacteria including bacteria of the genus Corynebacterium in the scalp flora, for example, any of the following indices may be used. · The ratio of bacteria of the genus Corynebacterium in the scalp flora · The ratio of bacteria of the genus Corynebacterium in the scalp flora among the total number of bacteria of Propionibacterium, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora · The total ratio of Propionibacterium, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora · An index obtained using the ratio of bacteria of the genus Corynebacterium in the scalp flora and the ratio of other bacteria in the scalp flora, and in which the ratio of bacteria of the genus Corynebacterium is weighted more than the ratio of other bacteria

[0041] As an index regarding the ratio of bacteria including bacteria of the genus Corynebacterium in the scalp flora, from the viewpoint of enhancing the reliability of the evaluation of the degree of the scalp barrier function, it is preferable to use the ratio of bacteria of the genus Corynebacterium in the scalp flora as an index.

[0042] The ratio of various bacteria in the scalp flora can be calculated using the number of bacteria grasped by the analysis of the scalp flora by the 16S rRNA sequencing method shown above.

[0043] (Comparison criteria) In the evaluation method of the first embodiment, it is preferable to evaluate by comparing the above index of the evaluated person with the comparison criteria of the index. According to this evaluation method, it becomes possible to evaluate the degree of the scalp barrier function of the evaluated person by comparing it with the scalp barrier function corresponding to the index of the comparison criteria. That is, by previously grasping the degree of the scalp barrier function corresponding to the index serving as the comparison criteria, it becomes possible to evaluate whether the degree of the scalp barrier function of the evaluated person is extremely different from the comparison criteria based on the relationship between the above index in the scalp flora of the evaluated person and the index serving as the comparison criteria.

[0044] This comparison criterion may be set, for example, as the degree of the scalp barrier function corresponding to the average value, median value, etc. of the above indicators of the general population. Further, multiple comparison criteria may be provided by dividing ranks for each of the above indicators of the general population. For example, when using, as the above indicator, "the total ratio of Propionibacterium acnes, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis in the scalp flora", the comparison criterion indicator may be set to 95%, and for the scalp where the indicator is 95% or more, it may be evaluated that the scalp barrier function is low. Also, for example, when using, as the above indicator, "the ratio of bacteria of the genus Corynebacterium in the scalp flora", the comparison criterion indicator may be set to 10%, and for the scalp where the indicator is 10% or more, it may be evaluated that the scalp barrier function is low.

[0045] Furthermore, for the same subject to be evaluated, by comparing the indicator in the current scalp flora with the indicator in the past scalp flora, the change trend of the degree of the scalp barrier function of the subject to be evaluated may be evaluated.

[0046] (1-2) Evaluation method of the second embodiment The evaluation method of the second embodiment is a method of evaluating at least one of the following indicators: an indicator of the diversity of bacteria in the scalp flora of the subject to be evaluated, an indicator related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the subject to be evaluated, and an indicator related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the subject to be evaluated, based on the degree of the scalp barrier function of the subject to be evaluated.

[0047] According to this evaluation method, it becomes possible to objectively evaluate the above indicators in the scalp flora of the subject to be evaluated. Note that this evaluation method can be used for cosmetic purposes and excludes medical acts performed by doctors and medical staff. Note that the scalp barrier function, the indicator of the diversity of bacteria in the scalp flora, the indicator related to the number of bacteria, and the indicator related to the ratio of bacteria are the same as those in the first embodiment above.

[0048] (Comparison criterion) In the evaluation method of the second embodiment, it is preferable to evaluate the degree of the barrier function of the scalp of the person to be evaluated by comparing it with a comparison standard for the degree of the barrier function of the scalp. According to this evaluation method, it becomes possible to evaluate the above index of the person to be evaluated by comparing it with an index corresponding to the degree of the barrier function of the scalp of the comparison standard. That is, by grasping in advance the above index corresponding to the barrier function of the scalp serving as the comparison standard, it becomes possible to evaluate the above index of the person to be evaluated from the relationship between the degree of the barrier function of the scalp of the person to be evaluated and the degree of the barrier function of the scalp serving as the comparison standard.

[0049] This comparison standard may be provided as the above index corresponding to the average value, median value, etc. of the degree of the barrier function of the scalp of the general population. Further, the comparison standards may be provided in a plurality by dividing ranks for each degree of the barrier function of the scalp of the general population. For example, when using the "number of bacteria of the genus Corynebacterium in the scalp flora" as the above index, the average degree of the barrier function of the scalp of the general population (TEWL score, etc.) and the corresponding number of bacteria of the genus Corynebacterium are grasped in advance as the comparison standard, and the number of bacteria of the genus Corynebacterium in the scalp of the person to be evaluated may be evaluated from the relationship between the degree of the barrier function of the scalp of the person to be evaluated (TEWL score, etc.) and the degree of the comparison standard.

[0050] Furthermore, for the same person to be evaluated, the change trend of the above index of the person to be evaluated may be evaluated by comparing the current degree of the barrier function of the scalp with the past degree of the barrier function of the scalp.

[0051] (3) Method for Selecting Scalp and Hair Cosmetics The method for selecting scalp and hair cosmetics is a method for selecting scalp and hair cosmetics to be recommended to the person to be evaluated according to the evaluation result obtained by the evaluation method of the first embodiment or the evaluation method of the second embodiment described above.

[0052] According to this method for selecting a scalp and hair cosmetic, it becomes possible to select a scalp and hair cosmetic suitable for the evaluated person according to the evaluation result of the degree of the barrier function of the scalp of the evaluated person. For example, for an evaluated person who is evaluated as having a low scalp barrier function, it is possible to suppress an increase in the number of bacteria on the scalp, suppress an increase in the number of bacteria of the genus Corynebacterium, or suppress a decrease in the diversity of bacteria in the scalp flora. A scalp and hair cosmetic containing components capable of such may be selected and its use may be recommended. Further, for example, it may be recommended to refrain from using a scalp and hair cosmetic containing a component that may increase the number of bacteria on the scalp, a scalp and hair cosmetic containing a component that may increase the number of bacteria of the genus Corynebacterium, or a scalp and hair cosmetic containing a component that may decrease the diversity of bacteria in the scalp flora. Here, the "scalp and hair cosmetic" means a cosmetic used for the scalp and / or hair.

[0053] In addition, a scalp and hair cosmetic to be recommended or a scalp and hair cosmetic for which use is to be refrained from according to an index such as the number of bacteria in the scalp flora may be previously distinguished and prepared as a list, and the selection of the scalp and hair cosmetic to be recommended may be made using the list.

[0054] Further, based on the evaluation result obtained by the evaluation method of the first embodiment or the evaluation method of the second embodiment described above, it is preferable to select a scalp and hair cosmetic containing a humectant. For example, for an evaluated person who is evaluated as having a high proportion of bacteria of the genus Corynebacterium in the scalp flora or an evaluated person who is evaluated as having a low scalp barrier function, it is recommended to use a hair cosmetic for the scalp containing a moisturizer. For an evaluated person who is evaluated as having a low proportion of bacteria of the genus Corynebacterium in the scalp flora or an evaluated person who is evaluated as having a high scalp barrier function, it is possible to recommend the use of a hair cosmetic for the scalp that is not limited to a hair cosmetic for the scalp containing a moisturizer. Even for a scalp with a high amount of water evaporation from the scalp and a tendency to dry due to a low scalp barrier function, it is possible to suppress scalp dryness by using a hair cosmetic for the scalp containing a moisturizer.

[0055] As the moisturizer, a known moisturizer used for the skin including the scalp can be used. Examples of the moisturizer include polyhydric alcohols (such as 1,3-butylene glycol and glycerin), saccharides (such as sorbitol), polysaccharides (such as hyaluronic acid), and animal and plant extracts (such as pearl calc extract and tormentilla extract).

[0056] The blending amount of the moisturizer in the hair cosmetic for the scalp is, for example, 0.01% by mass or more and 50% by mass or less. From the viewpoint of enhancing the moisturizing property of the scalp, the blending amount of the above moisturizer is preferably 0.1% by mass or more, and from the viewpoint of imparting appropriate moisturizing property to the scalp, it is preferably 30% by mass or less. Note that, according to the above evaluation results, for example, the blending amount may be determined so that the blending amount of the moisturizer in the hair cosmetic for the scalp is larger for an evaluated person who is evaluated as having a lower scalp barrier function.

[0057] Further, it is preferable to select a hair cosmetic for the scalp containing an anti-inflammatory agent based on the evaluation result obtained by the evaluation method of the first embodiment or the evaluation method of the second embodiment described above. For example, for an evaluated person who is evaluated as having a large number or proportion of bacteria of the genus Corynebacterium in the scalp flora, it is recommended to use a scalp and hair cosmetic containing an anti-inflammatory agent. For an evaluated person who is evaluated as having a small number or proportion of bacteria of the genus Corynebacterium in the scalp flora, it is possible to recommend the use of a scalp and hair cosmetic not limited to those containing an anti-inflammatory agent.

[0058] As the anti-inflammatory agent, one or more of β-glycyrrhetinic acid, dipotassium glycyrrhizinate, trisodium glycyrrhizinate, monoammonium glycyrrhizinate, pyridoxine hydrochloride, nicotinic acid, nicotinamide, and allantoin can be used.

[0059] The content of the anti-inflammatory agent in the scalp and hair cosmetic is preferably 0.05% by mass or more and 5% by mass or less, and more preferably 0.1% by mass or more and 2% by mass or less. In addition, according to the above evaluation results, the content of the anti-inflammatory agent in the scalp and hair cosmetic can be determined so that the higher the number or proportion of bacteria of the genus Corynebacterium in the scalp flora of the evaluated person, the higher the content of the anti-inflammatory agent in the scalp and hair cosmetic.

[0060] In addition, it is preferable to select a scalp and hair cosmetic containing an antibacterial agent based on the evaluation results obtained by the evaluation method of the first embodiment or the evaluation method of the second embodiment described above. For example, for an evaluated person who is evaluated as having a large number of bacteria in the scalp flora, it is recommended to use a scalp and hair cosmetic containing an antibacterial agent. For an evaluated person who is evaluated as having a small number of bacteria in the scalp flora, it is possible to recommend the use of a scalp and hair cosmetic not limited to those containing an antibacterial agent.

[0061] The antibacterial agent means a component that suppresses and / or inhibits the growth of bacteria and / or kills bacteria. As such an antibacterial agent, one or more of preservatives, surfactants, and chelating agents can be used.

[0062] Examples of preservatives include benzoic acid or its salts, undecylenic acid, salicylic acid, sorbic acid or its salts, dehydroacetic acid or its salts, paraoxybenzoic acid esters (e.g., methyl paraoxybenzoate or its salts, propyl paraoxybenzoate, isopropyl paraoxybenzoate, butyl paraoxybenzoate, isobutyl paraoxybenzoate, benzyl paraoxybenzoate, etc.), methylchloroisothiazolinone·methylisothiazolinone solution (aqueous solution containing methylchloroisothiazolinone and methylisothiazolinone), methylchloroisothiazolinone (5-chloro-2-methyl-4-isothiazolin-3-one), methylisothiazolinone (2-methyl-4-isothiazolin-3-one), phenol, isopropylmethylphenol, orthophenylphenol or its salts, parachlorophenol, thiobischlorophenol, cresol, p-chloro-m-cresol, chlorhexidine gluconate, chlorphenesin, chloroxylenol, dichloroxylenol, dichlorobenzyl alcohol, thymol, chlorothymol, trichlorocarbanilide, trichlorohydroxyphenyl ether, croflukanide, phenethyl alcohol, phenoxyethanol, hexachlorophene, photosensitizer No. 101, photosensitizer No. 201, photosensitizer No. 401, iodoparadimethylaminostyrylheptylmethylthiazolium, hinokitiol, (silver / copper) zeolite, glutaral, chloramine T, chlorhexidine, dibromopropamidine isethionate, zinc pyrithione, piroctone olamine, sodium pyrithione, 5-bromo-5-nitro-1,4-dioxane, polyaminopropyl biguanide, etc.

[0063] Examples of surfactants include one or more selected from cationic surfactants and anionic surfactants. Examples of cationic surfactants include quaternary ammonium salts (e.g., benzalkonium chloride, steartrimonium chloride, cetrimonium chloride, cetylpyridinium chloride, benzethonium chloride, lauryltrimonium chloride, laurylpyridinium chloride, etc.), cocoyl arginine ethyl PCA, etc. Examples of the anionic surfactant include sulfonic acid-based anionic surfactants (e.g., alkane sulfonates, α-olefin sulfonates, α-sulfo fatty acid methyl ester salts, acyl isethionates, alkyl glycidyl ether sulfonates, alkyl sulfosuccinates, alkyl naphthalene sulfonates, N-acyl methyl taurine salts, etc.), sulfuric acid-based anionic surfactants (e.g., alkyl sulfates (such as sodium lauryl sulfate), alkyl ether sulfates, alkyl aryl ether sulfates, fatty acid alkanolamide sulfates, fatty acid monoglyceride sulfates, etc.), phosphoric acid-based anionic surfactants (e.g., alkyl phosphates, polyoxyethylene alkyl ether phosphates, alkyl aryl ether phosphates, fatty acid amide ether phosphates, etc.).

[0064] Examples of the chelating agent include edetic acid, edetic acid salts (such as disodium edetate, trisodium edetate, tetrasodium edetate, dipotassium edetate, etc.), diethylenetriaminepentaacetic acid, diethylenetriaminepentaacetic acid salts (such as pentasodium diethylenetriaminepentaacetate, etc.), ethylenediamine hydroxyethyltriacetic acid, ethylenediamine hydroxyethyltriacetic acid salts (such as trisodium ethylenediamine hydroxyethyltriacetate, etc.), hydroxyethanediphosphonic acid, hydroxyethanediphosphonic acid salts (such as tetrasodium hydroxyethanediphosphonate, etc.).

[0065] The compounding amount of the antibacterial agent in the scalp and hair cosmetics may be appropriately set. Since it is advantageous for suppressing the increase in the number of bacteria, it is preferably 0.01% by mass or more, and more preferably 0.1% by mass or more. Also, from the viewpoint of suppressing skin irritation, the compounding amount of the antibacterial agent is preferably 2% by mass or less, and more preferably 1% by mass or less. In addition, the compounding amount may be determined such that the higher the number of bacteria in the scalp flora of the evaluated person is evaluated by the above evaluation method, the larger the compounding amount of the antibacterial agent in the scalp and hair cosmetics is.

[0066] When using a preservative as an antibacterial agent in a hair and scalp cosmetic, the blending amount of the preservative may be set as appropriate. Since it is advantageous for suppressing the increase in the number of bacteria, it is preferably 0.01% by mass or more, more preferably 0.1% by mass or more. Also, from the viewpoint of suppressing skin irritation, the blending amount of the preservative is preferably 2% by mass or less.

[0067] When using a surfactant as an antibacterial agent in a hair and scalp cosmetic, the blending amount of the surfactant may be set as appropriate. Since it is advantageous for suppressing the increase in the number of bacteria, it is preferably 0.1% by mass or more, more preferably 0.5% by mass or more. Also, from the viewpoint of suppressing skin irritation, the blending amount of the surfactant is preferably 20% by mass or less.

[0068] When using a chelating agent as an antibacterial agent in a hair and scalp cosmetic, the blending amount of the chelating agent may be set as appropriate. Since it is advantageous for suppressing the increase in the number of bacteria, it is preferably 0.01% by mass or more, more preferably 0.1% by mass or more. Also, the blending amount of the chelating agent is, for example, 1% by mass or less.

[0069] Examples of the types of the above-mentioned hair and scalp cosmetics include shampoos, rinses, conditioners, treatments (for example, non-rinse treatments, rinse-off treatments, treatments for hair styling, one component of a multi-agent treatment, treatments for pre-perming, treatments for post-perming, treatments for pre-coloring, treatments for post-coloring, treatments for pre-bleaching, treatments for post-bleaching), hair styling agents, hair growth agents, and the like. Examples of the dosage forms of such hair and scalp cosmetics include liquid, cream, gel, foam (bubbly), and mist.

[0070] In addition to the components corresponding to the above-mentioned moisturizing agents, anti-inflammatory agents, and antibacterial agents, the above-mentioned scalp and hair cosmetics may contain, as components used in scalp and hair cosmetics, for example, higher alcohols, lower alcohols, ester oils, fats and oils, fatty acids, hydrocarbons, waxes, silicones, high molecular compounds, amino acids, microbe-derived substances, inorganic compounds, fragrances, ultraviolet absorbers, and the like.

[0071] The above-mentioned scalp and hair cosmetics can be manufactured using known manufacturing methods for scalp and hair cosmetics. Moreover, the above-mentioned scalp and hair cosmetics can be used according to known usage methods for scalp and hair cosmetics.

[0072] (4) Screening method for scalp and hair cosmetics that improve the scalp barrier function A screening method for scalp and hair cosmetics that improve the scalp barrier function is a method of screening scalp and hair cosmetics using at least one of the following indicators: an index of the diversity of bacteria in the scalp microbiota of the subject, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject. The screening of scalp and hair cosmetics includes at least once: a step of treating the scalp and / or hair of the subject with the scalp and hair cosmetics, and a step of comparing the above indicators before and after the step of treating the scalp and / or hair.

[0073] According to this screening method, it becomes possible to identify scalp and hair cosmetics that can affect an index of the diversity of bacteria in the scalp microbiota, an index related to the number of bacteria including bacteria of the genus Corynebacterium, or an index related to the proportion of bacteria including bacteria of the genus Corynebacterium. As a result, it becomes possible to select scalp and hair cosmetics that can enhance the scalp barrier function, and to select scalp and hair cosmetics that are disadvantageous for enhancing the scalp barrier function. Note that scalp and hair cosmetics that cause a stronger degree of change in the above indicators of the subject can be selected as those that affect the above scalp barrier function. Note that the scalp barrier function, the index of the diversity of bacteria in the scalp flora, the index related to the number of bacteria, and the index related to the ratio of bacteria are the same as those described in the first embodiment above.

[0074] In this screening method, the more preferable the index used is, the stronger the correlation with the scalp barrier function is. Also, the number of times of treating the scalp and / or hair is not limited to once, and can be a predetermined number of times such as 30 times or 90 times, for example.

[0075] Here, the scalp and hair cosmetic is not particularly limited. For example, it may be the same type and dosage form of the scalp and hair cosmetic described in the above “(3) Method for selecting scalp and hair cosmetics”, and the components to be blended may also be the same as those described in the above “(3) Method for selecting scalp and hair cosmetics”. The method of treating the scalp and / or hair may be an appropriate method that can be normally performed.

[0076] (5) Screening method for candidate substances of active ingredients for improving scalp barrier function The screening method for candidate substances of active ingredients for improving the scalp barrier function is a method of screening candidate substances of active ingredients using at least one of the index of the diversity of bacteria in the scalp flora of the subject, the index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the subject, and the index related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the subject. In the screening of candidate substances of active ingredients, it includes at least once the step of treating the scalp and / or hair of the subject with the candidate substance of the active ingredient, and the step of comparing the above indexes before and after the step of treating the scalp and / or hair.

[0077] According to this screening method, it becomes possible to identify a hair cosmetic for the scalp that can affect an index of the diversity of bacteria in the scalp microbiota, an index related to the number of bacteria including bacteria of the genus Corynebacterium, or an index related to the ratio of bacteria including bacteria of the genus Corynebacterium. As a result, it becomes possible to select a candidate substance for an active ingredient that can enhance the barrier function of the scalp, and to select a candidate substance for an active ingredient that is disadvantageous for enhancing the barrier function of the scalp. Note that a candidate substance with a stronger degree of changing the above index of the subject to be evaluated can be selected as a substance that affects the barrier function of the scalp. Note that the barrier function of the scalp, the index of the diversity of bacteria in the scalp microbiota, the index related to the number of bacteria, and the index related to the ratio of bacteria are the same as those described in the first embodiment above.

[0078] The index used in this screening method is preferably an index with a stronger correlation with the barrier function of the scalp. Also, the number of times of treating the scalp and / or hair is not limited to once, and can be a predetermined number of times such as 30 times, 90 times, etc.

[0079] (6) Method for manufacturing a hair cosmetic for the scalp The method for manufacturing a hair cosmetic for the scalp is to formulate an active ingredient selected as a candidate substance for an active ingredient that can affect the above index and enhance the barrier function of the scalp in the above “(5) Screening method for candidate substances for active ingredients that improve the barrier function of the scalp”. According to this manufacturing method, a hair cosmetic for the scalp that can improve the barrier function of the scalp can be obtained.

[0080] Note that the hair cosmetic for the scalp to be manufactured is not particularly limited, and may be, for example, a hair cosmetic for the scalp of the same type and dosage form as described in the above “(3) Selection method for hair cosmetics for the scalp”, and the blending components other than the active ingredient may be the same as those described in the above “(3) Selection method for hair cosmetics for the scalp”.

[0081] Incidentally, the active ingredient incorporated into the scalp and hair cosmetic can be, for example, one or more of the humectants, anti-inflammatory agents, and antibacterial agents described in the above “(3) Method for Selecting Scalp and Hair Cosmetics”, and the blending amount can also be within the range described therein.

[0082] (7) Scalp and hair cosmetics The scalp and hair cosmetic is a scalp and hair cosmetic selected as one that improves the barrier function of the scalp by the above “(4) Screening Method for Scalp and Hair Cosmetics that Improve the Barrier Function of the Scalp”, or an effective ingredient selected as one that improves the barrier function of the scalp by the above “(5) Screening Method for Candidate Substances of Active Ingredients that Improve the Barrier Function of the Scalp” is incorporated. According to this scalp and hair cosmetic, it becomes possible to improve the barrier function of the scalp.

[0083] Incidentally, regarding the barrier function of the scalp, the index of the diversity of bacteria in the scalp flora, the index regarding the number of bacteria, and the index regarding the ratio of bacteria, they are the same as the descriptions in the above First Embodiment. Also, the scalp and hair cosmetic is not particularly limited, and for example, it may be a scalp and hair cosmetic of the same type and dosage form as those described in the above “(3) Method for Selecting Scalp and Hair Cosmetics”, and the ingredients to be incorporated may also be the same as those described in the above “(3) Method for Selecting Scalp and Hair Cosmetics”.

[0084] (8) Evaluation support tool for assisting in the evaluation of the degree of the barrier function of the scalp The evaluation support tool for assisting in the evaluation of the degree of the barrier function of the scalp of the subject to be evaluated includes an acquisition unit, a storage unit, a comparison unit, and a display unit. The acquisition unit acquires index specifying information. The index specifying information is information for specifying an index, and examples thereof include information used for calculating the value of the index and the like.

[0085] At least one of an index indicating the diversity of bacteria in the scalp microbiota of the subject, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and an index related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject is used. Note that the scalp barrier function, the index indicating the diversity of bacteria in the scalp microbiota, the index related to the number of bacteria, and the index related to the ratio of bacteria are the same as those described in the first embodiment above.

[0086] The storage unit stores information serving as a comparison reference for the index. The comparison unit compares the index of the subject specified from the index specification information acquired by the acquisition unit with the comparison reference of the index stored in the storage unit. The display unit displays and outputs the comparison result by the comparison unit.

[0087] According to this evaluation support tool, the index of the subject specified from the index specification information is compared with the reference index, and the result is displayed and output. Therefore, it is supported to evaluate the degree of the scalp barrier function of the subject with respect to the degree of the scalp barrier function corresponding to the reference index. More specifically, it is supported to evaluate whether the degree of the scalp barrier function of the subject is better than the degree of the scalp barrier function corresponding to the reference index based on the relationship between the index of the subject and the reference index.

[0088] The evaluation support tool is not particularly limited, and for example, it may be the evaluation support tool 1 shown in the block diagram of FIG. 14. This evaluation support tool 1 includes an acquisition unit 10 including a keyboard, a mouse, a touch panel, etc. that accepts input of the above-mentioned index identification information, a storage unit 20 including a ROM, etc. that stores information 21 serving as a comparison standard for the index, and a CPU 31 that identifies the index of the person to be evaluated from the index identification information and performs various information arithmetic processes such as comparing with the comparison standard of the index. It may be an information processing device including a processor, a RAM 32, a ROM 33, etc. that stores a program, etc., and a comparison unit 30, and a display unit 40 such as a display that displays and outputs the comparison result by the comparison unit 30. Note that this evaluation support tool 1 may be configured to be able to obtain information serving as a comparison standard for the index from an external server or the like through communication via a network.

[0089] (9) An evaluation support tool for supporting the evaluation of an index related to the scalp bacterial flora The evaluation support tool for supporting the evaluation of an index related to the scalp bacterial flora includes an acquisition unit, a storage unit, a comparison unit, and a display unit.

[0090] The index related to the scalp bacterial flora is at least any one of an index of the diversity of bacteria in the scalp bacterial flora of the person to be evaluated, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp bacterial flora of the person to be evaluated, and an index related to the ratio of bacteria including at least bacteria of the genus Corynebacterium in the scalp bacterial flora of the person to be evaluated. Note that the scalp barrier function, the index of the diversity of bacteria in the scalp bacterial flora, the index related to the number of bacteria, and the index related to the ratio of bacteria are the same as those described in the first embodiment above.

[0091] The acquisition unit acquires barrier function specific information. The barrier function specific information is information for specifying the degree of the barrier function of the scalp of the person to be evaluated, and examples thereof include information used for calculating a value indicating the degree of the barrier function of the scalp. The storage unit stores information serving as a comparison reference for the degree of the barrier function of the scalp. The comparison unit compares the degree of the barrier function of the scalp of the person to be evaluated specified from the barrier function specific information acquired by the acquisition unit with the comparison reference for the degree of the barrier function of the scalp stored in the storage unit. The display unit displays and outputs the comparison result by the comparison unit.

[0092] According to this evaluation support tool, since the degree of the barrier function of the scalp of the person to be evaluated specified from the barrier function specific information is compared with the barrier function of the scalp as the comparison reference, and the result is displayed and output, it is supported to evaluate the index of the person to be evaluated with respect to the index corresponding to the degree of the barrier function of the scalp as the comparison reference. More specifically, based on the relationship between the degree of the barrier function of the scalp of the person to be evaluated and the degree of the barrier function of the scalp as the comparison reference, it is supported to evaluate the relationship (such as the magnitude relationship of values) between the index of the person to be evaluated and the index corresponding to the degree of the barrier function of the scalp as the comparison reference.

[0093] The evaluation support tool is not particularly limited, and for example, it may be an evaluation support tool 2 as shown in the block configuration diagram of FIG. 15. This evaluation support tool 2 includes an acquisition unit 50 including a keyboard, a mouse, a touch panel, etc. that accepts the input of the above-described barrier function specific information, a storage unit 60 including a ROM, etc. that stores information 61 serving as a comparison reference for the degree of the barrier function of the scalp, and a processor such as a CPU 71 that performs various information arithmetic processes of specifying the degree of the barrier function of the scalp of the person to be evaluated from the barrier function specific information and comparing it with the comparison reference for the degree of the barrier function of the scalp, a RAM 72, and a ROM 73, etc. that stores a program, etc., and may be an information processing apparatus including a comparison unit 70 and a display unit 80 such as a display that displays and outputs the comparison result by the comparison unit 70. Note that this evaluation support tool 2 may be configured such that information serving as a comparison reference for the degree of the barrier function of the scalp can be obtained from an external server or the like through communication via a network.

Example

[0094] (Regarding the correlation between the trans-epidermal water loss (TEWL) and bacteria in the scalp microbiota) In accordance with the Helsinki Declaration, 276 subjects aged 20 to 79 years (Japanese women classified as Mongoloid) who consented to the consent form approved by the ethics committee were targeted. For each subject, the trans-epidermal water loss (TEWL) in the scalp other than the partings and around the whorls in the top region of the scalp was measured using the Tewameter TM HEX (manufactured by Courage+Khazaka) of the water evaporation meter.

[0095] Among the measured subjects, the group with the top 15% of TEWL scores was defined as the high-TEWL group (40 subjects), and the group with the bottom 15% of TEWL scores was defined as the low-TEWL group (40 subjects). The TEWL scores of the high-TEWL group and the low-TEWL group are shown in the box-and-whisker plot of Fig. 1. The vertical axis in Fig. 1 indicates the TEWL score, and the unit is g / m 2 / h. The box indicates the range of 1 / 4 or more and 3 / 4 or less at low or high scores. The thick line in the box indicates the average value at low or high scores, and it shows that 95% of the data exists between the upper and lower lines of the box (the same applies to each box-and-whisker plot below). Here, the high-TEWL group is a group with a large amount of water permeating from the stratum corneum of the scalp to the outside, and can be said to be a group with a low scalp barrier function. Also, the low-TEWL group is a group with a small amount of water permeating from the stratum corneum of the scalp to the outside, and can be said to be a group with a high scalp barrier function.

[0096] Next, in order to examine the relationship between the number of scalp bacteria, etc. and TEWL (scalp barrier function), medical adhesive tape of a certain area was pressed against the scalp other than the partings and around the whorls in the top region of the scalp for subjects belonging to the high-TEWL group and the low-TEWL group to collect the scalp microbiota from the scalp.

[0097] For each sample of the scalp microbiota taken, analysis was performed by the 16S rRNA sequencing method according to the procedures shown in the following [1] to [4]. [1] Nucleic acids of the scalp microbiota collected from the adhesive tape were extracted by a conventional method. [2] Using the extracted nucleic acids, the 16S rRNA gene was amplified by the PCR method. [3] Using the amplified 16S rRNA gene, DNA analysis of the 16S rRNA gene was performed with a next-generation sequencer (MiSeq (trade name) manufactured by Illumina) (primers used: 27Fmod-338R manufactured by Biotechnology Research Institute Co., Ltd.). [4] From the obtained analysis results, the QIIME analysis tool was used to determine the percentage of the presence of various bacteria in the collected scalp microbiota.

[0098] In addition, separately from the analysis by the 16S rRNA sequencing method, for each sample of the scalp microbiota taken, the absolute number of each bacterium was confirmed by measuring the copy number (DNA amount) of each bacterium according to the procedures shown in the following <1> to <3>. <1> The scalp microbiota collected from the medical adhesive tape was completely lysed to purify the bacterial DNA. <2> To confirm the amount of purified DNA, the quantitative PCR method was used. <3> For each of Propionibacterium acnes, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis, primers that specifically match a specific base sequence were designed and used to calculate the DNA copy number. At this time, in order to confirm the absolute amount of the DNA copy number of the bacterium, the absolute number of the standard was also confirmed at the same time, and the calculation was performed by comparing with that value. The calculated copy number of each bacterium was taken as the amount of bacteria possessed by each subject in a certain area.

[0099] Using the TEWL value of each subject and the information on the scalp microbiota (the percentage of the presence of various bacteria in the scalp microbiota, the absolute number of bacteria in the scalp microbiota), multiple regression analysis by partial correlation was performed using R (statistical analysis software).

[0100] As information on the scalp microbiota, analysis was performed on the Shannon index, total bacterial count, total bacterial count of Propionibacterium acnes, Corynebacterium spp., and Staphylococcus epidermidis, the bacterial count of Propionibacterium acnes, the bacterial count of Corynebacterium spp., the bacterial count of Staphylococcus epidermidis, the ratio of the bacterial count of Propionibacterium acnes to the total bacterial count, the ratio of the bacterial count of Corynebacterium spp. to the total bacterial count, and the ratio of the bacterial count of Staphylococcus epidermidis to the total bacterial count, respectively. The Shannon index was calculated by the formula of Shannon Index H' using the abundance ratios of various bacteria in the scalp microbiota.

[0101] A graph showing the analysis results of the scalp microbiota of the group with high TEWL and the group with low TEWL described above is shown in Figure 2. In this graph, the average ratio of each bacterium in the subjects with high TEWL and the average ratio of each bacterium in the subjects with low TEWL are shown, respectively. Regarding the graph of the group with high TEWL, the higher the ratio of the bacterium, the more to the left it is located, and the graph of the group with low TEWL shows the bacteria arranged in the same order as the graph of the group with high TEWL.

[0102] Box plots using the Shannon index, total bacterial count, total bacterial count of Propionibacterium acnes, Corynebacterium spp., and Staphylococcus epidermidis, the bacterial count of Propionibacterium acnes, the bacterial count of Corynebacterium spp., the bacterial count of Staphylococcus epidermidis, the ratio of Propionibacterium acnes, the ratio of Corynebacterium spp., and the ratio of Staphylococcus epidermidis in the scalp microbiota as indicators for the group with high TEWL and the group with low TEWL are shown in Figures 3 to 11, respectively. In addition, if the p-value obtained from the results is less than 0.05, there is a significant difference, and it is considered that there is a positive correlation between the index and the TEWL score (scalp barrier function).

[0103] Although it is known that the scalp microbiota generally accounts for nearly 90% of the whole with Propionibacterium acnes, Corynebacterium spp., and Staphylococcus epidermidis, the relationship between such scalp microbiota and the TEWL score (scalp barrier function) has not been clarified at all conventionally. In contrast, according to the above analysis, in the scalp microbiota, the Shannon index, which is an index of bacterial diversity in the scalp microbiota, the total bacterial count, the total bacterial count of Propionibacterium acnes, Corynebacterium spp., and Staphylococcus epidermidis, the bacterial count of Corynebacterium spp., and the ratio of the bacterial count of Corynebacterium spp. to the total bacterial count were all found to be correlated with the TEWL score, which is an example of the scalp barrier function.

[0104] Specifically, it was confirmed that the lower the TEWL score (the higher the scalp barrier function), the higher the Shannon index in the scalp microbiota (Figure 3).

[0105] Also, it was confirmed that the higher the TEWL score (the lower the scalp barrier function), the higher the total bacterial count, and the higher the TEWL score (the lower the scalp barrier function), the higher the total bacterial count of Propionibacterium acnes, Corynebacterium spp., and Staphylococcus epidermidis (Figures 4 and 5). In particular, it was confirmed that the TEWL score shows a more significant difference in the total bacterial count of Propionibacterium acnes, Corynebacterium spp., and Staphylococcus epidermidis compared to the total bacterial count (p-value comparison in Figures 4 and 5).

[0106] Furthermore, it was confirmed that the lower the TEWL score (the higher the scalp barrier function), the lower the bacterial count of Corynebacterium spp., and the lower the TEWL score (the higher the scalp barrier function), the smaller the ratio of the bacterial count of Corynebacterium spp. to the total bacterial count (Figures 7 and 10). On the other hand, although it is generally the case that the bacterial count and ratio are the highest on the scalp, no positive correlation was observed between the bacterial count of Propionibacterium acnes and the ratio of the bacterial count of Propionibacterium acnes to the total bacterial count with the TEWL score (Figures 6 and 9). Also, no positive correlation was observed between the ratio of the bacterial count of Staphylococcus epidermidis to the total bacterial count and the TEWL score (Figure 11). Although a positive correlation was observed between the bacterial count of Staphylococcus epidermidis and the TEWL score, no significant difference was found as in the case of the bacterial count of Corynebacterium spp. (p-value comparison in Figures 7 and 8).

[0107] Although the above-mentioned correlations were confirmed between each index and the TEWL score, no correlation was confirmed between the TEWL score and the redness (a * value) of the scalp.

[0108] (Regarding Trans-epidermal Water Loss (TEWL) and Multilayer Exfoliation of Keratinocytes) Using medical adhesive tape of a certain area, the subjects were divided into two groups: those with high TEWL and those with low TEWL. The tape was pressed against the scalp, excluding the parting and the area around the cowlick in the top region of the scalp, to exfoliate keratinocytes from the scalp (tape stripping). Subsequently, the stratum corneum cells peeled off from the medical adhesive tape were transferred and fixed onto a slide glass, and then immersed in an aqueous solution of 1% by mass gentian violet and 0.5% by mass brilliant green at room temperature for 15 minutes. After immersion, they were washed with running tap water and air-dried. The exfoliated keratinocytes on the slide glass were observed using an all-in-one fluorescence microscope BZ-X810 (manufactured by Keyence Corporation). Microscopic photographs of tape stripping for subjects in the high TEWL group are shown in Fig. 12, and those for subjects in the low TEWL group are shown in Fig. 13.

[0109] In the above tape stripping, in the scalp with good barrier function, the stratum corneum cells are exfoliated layer by layer. In the subjects belonging to the high TEWL group, it was confirmed that there were more cases of multilayer exfoliation where the stratum corneum cells of the scalp were exfoliated in multiple layers compared to the subjects belonging to the low TEWL group. Thus, it can be seen that the scalp prone to multilayer exfoliation shows a similar tendency of scalp bacterial flora to that in the case of high TEWL, and the scalp less prone to multilayer exfoliation shows a similar tendency of scalp bacterial flora to that in the case of low TEWL.

Claims

1. Based on at least any one of an index of the diversity of bacteria in the scalp microbiota of the subject, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, evaluate the degree of the barrier function of the scalp of the subject, or Based on the degree of the barrier function of the scalp of the subject, evaluate at least any one of an index of the diversity of bacteria in the scalp microbiota of the subject, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, Evaluation method.

2. The index of the diversity of the bacteria is at least any one of the number of types of bacteria present on the scalp and the evenness of the proportion of each type of bacteria present on the scalp, The index related to the number of bacteria is at least any one of the number of bacteria of the genus Corynebacterium in the scalp microbiota of the subject, the total number of bacteria of Propionibacterium acnes, bacteria of the genus Corynebacterium, and Staphylococcus epidermidis, and the total number of bacteria, The index related to the proportion of the bacteria is the proportion of bacteria of the genus Corynebacterium in the scalp microbiota of the subject, The evaluation method according to claim 1.

3. Evaluate the degree of the barrier function of the subject by comparing the index of the subject with a comparison criterion of the index, or Evaluate the index of the subject by comparing the degree of the barrier function of the subject with a comparison criterion of the degree of the barrier function, The evaluation method according to claim 1 or 2.

4. A method for selecting a scalp and hair cosmetic recommended for the subject according to the evaluation result obtained by the evaluation method of claim 1 or 2.

5. A step of treating the scalp and / or hair of the subject with a scalp and hair cosmetic at least once, and Before and after the step of treating the scalp and / or hair, An index of the diversity of bacteria in the scalp microbiota of the subject, An index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, An index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp microbiota of the subject, A step of comparing at least any one of the indicators; A screening method for a scalp hair cosmetic that improves the barrier function of the scalp provided with the above.

6. A step of treating the scalp and / or hair of an evaluated person with a candidate substance for an active ingredient at least once; Before and after the step of treating the scalp and / or hair, An index of the diversity of bacteria in the scalp flora of the evaluated person; An index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person; An index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person; A step of comparing at least any one of the indicators; A screening method for a candidate substance for an active ingredient that improves the barrier function of the scalp provided with the above.

7. A method for producing a scalp hair cosmetic, which contains an active ingredient selected by the screening method according to Claim 6. A method for producing a scalp hair cosmetic.

8. A scalp hair cosmetic containing a candidate substance for an active ingredient selected by the screening method according to Claim 5 or a candidate substance for an active ingredient selected by the screening method according to Claim 6.

9. An evaluation support tool for supporting the evaluation of the degree of the barrier function of the scalp of an evaluated person, An acquisition unit that acquires index identification information for identifying at least any one of an index of the diversity of bacteria in the scalp flora of the evaluated person, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person; A storage unit that stores information serving as a comparison criterion for the index; A comparison unit that compares the index of the evaluated person specified from the index identification information acquired by the acquisition unit with the comparison criterion of the index stored in the storage unit; A display unit that displays and outputs the comparison result by the comparison unit; An evaluation support tool provided with the above.

10. An evaluation support tool for supporting the evaluation of at least any one of an index of the diversity of bacteria in the scalp flora of an evaluated person, an index related to the number of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person, and an index related to the proportion of bacteria including at least bacteria of the genus Corynebacterium in the scalp flora of the evaluated person. An acquisition unit that acquires barrier function identification information for identifying the degree of the scalp barrier function of the subject; A storage unit that stores information serving as a comparison criterion for the degree of the scalp barrier function; A comparison unit that compares the degree of the scalp barrier function of the subject identified from the barrier function identification information acquired by the acquisition unit with the comparison criterion for the degree of the scalp barrier function stored in the storage unit; A display unit that displays and outputs the comparison result by the comparison unit; An evaluation support tool comprising the above.

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