Viral delivery of GRNA to the scion

The virus-mediated delivery of gRNAs and Cas nucleases directly to soybean meristems provides an efficient and heritable genome editing method, overcoming the limitations of transgene introduction and recalcitrant transformation in soybeans.

WO2025076141A1 Publication Date: 2025-04-10INARI AGRICULTURE TECHNOLOGY INC +5
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Patent Information

Application Number
PCT/US2024/049665
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-04-30
Filing Date
2024-10-02
Publication Date
2025-04-10

AI Technical Summary

Technical Problem

Current methods for genome editing in plants, particularly in soybeans, are limited by the need for transgene introduction into the meristem, which is recalcitrant to transformation and requires lengthy tissue culture and plant regeneration processes.

Method used

A method involving virus-mediated delivery of guide RNAs (gRNAs) and Cas nucleases to the meristem cells of soybean plants, using a soybean-infecting virus, to induce both somatic and heritable mutations without introducing a transgene into the meristem genome.

Benefits of technology

This method allows for efficient and heritable genome editing in soybean meristems, bypassing the need for transgenic lines and reducing the time and complexity of the editing process.

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Abstract

The present disclosure provides methods for editing a genomic target in a soybean plant through the use of viral-mediated delivery of at least one genome editing reagent. Also provided are methods of editing a grafted scion by expression in the rootstock of a meristem transport segment- tagged endonuclease, and delivery of additional genome editing reagents to the plant or to the scion by virus-mediated delivery. Also provided are viral vector systems for use in the methods herein, and soybean plants, seeds, and cells edited using the provided methods.
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