Living cell atomization and inhalation apparatus and use method

Abstract

The present application provides a living cell atomization and inhalation apparatus and a use method. The method comprises the following steps: S1, culturing in vitro living cells that need to be atomized and inhaled; S2, carrying out related quality tests on the living cells cultured in vitro, and identifying a specific living cell marker; S3, centrifuging the living cells cultured in vitro, removing the supernatant, and then adding a certain amount of living cell culture solution and pulmonary cell protecting solution to resuspend the living cells; S4, cryopreserving the living cells; S5, thawing the living cells; and S6, preparing a cell atomization agent, and carrying out continuous atomization by means of an atomization apparatus. In the present application, by means of in-vitro cell culture, the improvement to the in-vitro cell culture medium can improve the cell activity after cell atomization; the arranged atomization and inhalation apparatus has the characteristic of portability; pulmonary inhalation after cell atomization solves the clinical delivery pain point in current clinical cell therapy.

Description

Living cell atomization inhalation device and usage method Technical Field

[0001] The present invention relates to the field of atomization technology, and in particular to a living cell atomization inhalation device and a use method thereof. Background Art

[0002] Cell therapy is widely used in the fields of multi-tissue regeneration and organ defects. However, its treatment methods are often achieved through surgical treatment, exosome delivery, vascular input, in situ surgical injection, etc., resulting in the technical threshold of cell therapy being completed only through medical institutions. Therefore, there are many technical solutions for the methods and schemes of cell input into the human body. At present, there is no good technical research on portable and home-based cell input methods. At present, the most common methods of cell input are intravenous injection, surgical injection in situ of defective organs, or preparing cells into powder or their derivatives (such as exosomes) and then inputting them into the body through atomization or oral administration. Cell activity preservation and cell input both have the disadvantage of too high a technical threshold. Therefore, it is hoped to develop a set of methods to improve the cell inhalation / input method so that the technology can be better promoted to commercialization and portability. The establishment of this method will be beneficial to the development or application of cell therapy or cell health care industry. For example: The Chinese invention patent (application number: CN201910747093.7) discloses "Stem cell active peptide atomization inhalation method for the treatment of pulmonary fibrosis and preparation of atomizers", and its description discloses: Studies have shown that most of the active peptides secreted by stem cells can fuse well with the cell membrane, thereby transporting the internal active substances to the interior of the targeted cells to exert their effects. At present, there have been reports on the application of mesenchymal stem cells in the repair of lung injury and pulmonary fibrosis, but due to the short survival time of active cells and the harsh storage and transportation conditions, the effect is limited; the above patent can prove the defects of the existing technology. Therefore, we have made improvements to this and proposed a living cell atomization inhalation device and method of use.

[0003] Summary of the Invention

[0004] The purpose of the present invention is to address the current problems of high technical barriers to cell viability preservation and cell transfusion. To achieve the above-mentioned purpose, the present invention provides the following living cell atomization inhalation device and method of use to improve the above-mentioned problems. The specific application is as follows:

[0005] A method for using a live cell aerosol inhalation device comprises the following steps: S1, culturing live cells to be aerosolized and inhaled in vitro; S2, performing relevant quality inspections on the live cells cultured in vitro and identifying specific live cell markers; S3, discarding the supernatant of the live cells cultured in vitro by centrifugation, then adding a certain amount of live cell culture fluid and lung cell protective fluid for resuspending; S4, freezing the live cells; S5, reviving the live cells; and S6, preparing a cell aerosol agent and performing continuous aerosolization through the aerosol device.

[0006] As a preferred technical solution of the present application, the living cell freezing comprises the following steps: S1. preparing a freezing solution with a protective agent concentration of 2 times the final concentration and half the final volume, slowly adding the freezing solution to the culture medium along the wall of the tube, and placing it in a specific environment for more than 10 minutes; S2. taking 10 μl of the cell suspension to be frozen and counting, calculating the cell density and viability, and adding a certain amount of preservation solution to the centrifuge tube based on the cell counting results so that the cell density in the tube is twice the freezing density; S3. slowly adding a volume of cell freezing solution equal to the volume of preservation solution added to the tube to the cell suspension along the wall of the tube, gently pipetting and mixing to make the cell density consistent with the instruction requirements; S4. dividing the cell suspension into freezing tubes; S5. placing the cells to be frozen in a freezing box filled with isopropyl alcohol, letting them stand in a specific environment for a period of time, and then transferring them to a liquid nitrogen tank.

[0007] As a preferred technical solution of the present application, the living cell cryopreservation also includes cryopreservation tube labeling and sampling detection.

[0008] As a preferred technical solution of this application, the living cell recovery includes the following steps:

[0009] S1. Adjust the temperature of the constant temperature water bath to a certain range. S2. Take out the cell cryopreservation tube from the liquid nitrogen tank and immediately place it in warm water and gently shake it until the cryopreservation solution is completely dissolved.

[0010] As a preferred technical solution of the present application, the freezing solution used in the living cell freezing step is 10% DMSO, 5% human serum albumin, 1% dextran and compound electrolyte injection.

[0011] A living cell atomization inhalation device includes a shell, a mounting assembly is provided at the bottom of the shell, a connecting portion and a transfer portion are provided on one side of the shell, and the connecting portion is located directly above the transfer portion, an atomization portion is provided on the top of the connecting portion, and a driving portion, an input portion and an output portion are provided inside the shell, the output portion is located on a side close to the transfer portion, and the driving portion is located between the input portion and the output portion.

[0012] Compared with the prior art, the present invention has the following beneficial effects:

[0013] 1. In order to solve the problem of high technical threshold for preserving live cell activity in the existing technology, this application improves the cell activity problem after cell atomization by culturing live cells in vitro and improving the in vitro culture medium for live cells;

[0014] 2. To address the problem of high technical barriers to entry in live cell transfusion in existing technologies, this application proposes a portable atomization inhalation device that allows live cells to be atomized and then inhaled into the lungs, thus resolving the current pain point of clinical transfusion in live cell therapy.

[0015] 3. The function of quantitative liquid delivery is achieved by cooperating with the driving part, transfer part and connecting part. By accurately controlling the delivery amount of liquid, the waste caused by excessive or insufficient use of liquid can be reduced. The operation is simple and suitable for personal operation.

[0016] 4. The automatic fixing function is achieved by cooperating with the installation component and the driving part. During the operation of the driving part, the tank body in the transfer part structure can be automatically fixed. The disassembly and assembly operation is simple and convenient for use.

[0017] 5. The drive unit, input unit and output unit are coordinated and assembled through structural changes to achieve the centrifugal separation function. It can be applied to cell centrifugation and has diversified functions.

[0018] 6. The speed limiting function is achieved by coordinating the transmission switching component with the moving component. If the driving part runs too fast, the power transmission can be automatically cut off to improve the operation stability. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] FIG1 is a flow chart of a method for using the live cell atomization inhalation device provided by the present application;

[0020] FIG2 is an experimental and data diagram of Example 2 in this application;

[0021] FIG3 is a second experimental and data diagram of Example 2 in this application;

[0022] FIG4 is the experimental and data diagram 3 of Example 2 in this application;

[0023] FIG5 is an experimental and data diagram of Example 2 in this application;

[0024] FIG6 is an experimental and data diagram 5 of Example 2 of this application;

[0025] FIG7 is an experimental and data diagram of Example 2 in this application;

[0026] FIG8 is a front view of the living cell atomization inhalation device provided by the present application;

[0027] FIG9 is a schematic structural diagram of the living cell atomization inhalation device provided by the present application;

[0028] Figure 10 is an enlarged view of A in this application;

[0029] FIG11 is a schematic structural diagram of a No. 1 transmission rod and a No. 2 transmission rod in the living cell atomization inhalation device provided by the present application;

[0030] FIG12 is a schematic diagram of the structure of the movable bar and the mounting tube in the living cell atomization inhalation device provided by the present application;

[0031] FIG13 is a schematic diagram of the internal structure of the mounting tube in the living cell atomization inhalation device provided by the present application;

[0032] FIG14 is a schematic structural diagram of a moving component in the living cell atomization inhalation device provided by the present application;

[0033] FIG15 is a cross-sectional view of the housing of the living cell aerosol inhalation device provided by the present application;

[0034] Figure 16 is an enlarged view of C in this application;

[0035] FIG17 is a schematic structural diagram of the installation components of the living cell atomization inhalation device provided by the present application;

[0036] Figure 18 is an enlarged view of D in this application;

[0037] Figure 19 is an enlarged view of E in this application;

[0038] Figure 20 is an enlarged view of B in this application;

[0039] FIG21 is a schematic structural diagram of a pressure conversion assembly in the living cell atomization inhalation device provided by the present application;

[0040] FIG22 is a schematic diagram of the structure of the ring in the living cell atomization inhalation device provided by the present application;

[0041] FIG23 is a schematic diagram of the structure of the support bar in the living cell atomization inhalation device provided by the present application;

[0042] FIG24 is a schematic structural diagram of the top cover of the living cell atomization inhalation device provided by the present application;

[0043] FIG25 is a schematic diagram of the structure of the support rod and the stabilizing rod in the living cell atomization inhalation device provided in this application.

[0044] 1. Mounting assembly; 11. Base; 12. Switch assembly; 121. Knob; 122. Threaded rod; 123. Rotating ring; 124. Sealing plug; 125. Connecting rod No. 1; 126. Movable rod; 127. Support ring; 128. Connecting rod No. 2; 13. Negative pressure fixing assembly; 131. Cylinder; 132. Winding wheel; 134. Spring No. 1; 135. Piston No. 1; 136. Pull rope; 137. Locking hook; 138. Threaded cylinder; 139. Mounting seat; 1310. Clamping rod; 14. Exhaust hole; 15. Cavity No. 1; 16, Cavity No. 2; 17, Connecting hole No. 1; 18, Cavity No. 3; 19, Connecting hole No. 2; 110, Cavity No. 4; 111, Connecting hole No. 3; 112, Cavity No. 5; 2, Housing; 21, Partition No. 1; 22, Partition No. 2; 3, Connecting portion; 31, Connecting platform; 32, Press switch; 33, Output channel; 34, Pressure conversion assembly; 341, Housing; 342, Spring No. 2; 343, Input port; 344, Output port; 345, Slide switch; 346, Limit rod; 35, Spring No. 3; 36. Elastic buckle; 37. Card slot; 4. Transfer part; 41. Tank; 42. Hose; 43. End cover; 44. Spring No. 4; 45. Output head; 5. Atomizing part; 51. Atomizer; 52. Connector; 54. Filter; 6. Side cover; 7. Drive unit; 71. Piston No. 2; 72. Press head; 73. Transmission rod No. 1; 74. Transmission rod No. 2; 75. Transmission switching assembly; 751. Push rod; 752. Connecting rod; 753. Slider No. 1; 754. Movable bar; 755. Mounting cylinder; 756. Slide; 757. Slider No. 2; 758. Ball head buckle No. 1; 759. Limiting ring; 7510. Spring No. 5; 7511. Connecting rope; 76. Moving assembly; 761. Moving platform; 763. Spring No. 6; 764. Telescopic block; 7 65. Drive block No. 1; 766. Drive block No. 2; 8. Input part; 81. One-way valve; 82. Knob column; 83. Hollow rod; 84. Support rod; 85. Ring; 86. Slider No. 4; 87. Slider No. 5; 88. Filter; 89. Inner ring; 810. Support bar; 811. Ball joint buckle No. 2; 812. Spring No. 7; 813. Pull rod; 814. Operating block; 815. Top cover; 816. Stabilizer bar; 817. Spring No. 8; 818. Ball joint buckle No. 3; 9. Output part; 91. Drive rod; 92. Rectangular frame; 93. Triangle block No. 1; 94. Transmission plate; 95. Triangle block No. 2; 96. Movable frame; 97. Rack; 98. Strip-shaped through hole; 99. Pressure head; 910. Sleeve; 911. Telescopic rod; 912. Linkage rod. DETAILED DESCRIPTION

[0045] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts should fall within the scope of protection of the present invention.

[0046] In order to solve this technical problem, the present invention provides a living cell atomization inhalation device and a method of use.

[0047] Example 1

[0048] Referring to Figure 1, a method for using a live cell aerosol inhalation device specifically includes the following steps: S1. Culturing live cells to be aerosolized inhaled in vitro; S2. Subjecting the cultured live cells to relevant quality inspections and identifying specific live cell markers; S3. Centrifuging the cultured live cells to discard the supernatant, then adding a certain amount of live cell culture fluid and lung cell protective fluid for resuspending; S4. Cryopreserving the live cells; S5. Resuscitating the live cells; S6. Preparing a cell aerosol and continuously aerosolizing it through the aerosol device. Live cells include, but are not limited to, mesenchymal stem cells, immune cells, mature tissue cells differentiated from umbilical cord mesenchymal stem cells, tissue cells differentiated from IPS pluripotent stem cells, CART cells, and CAR-NK cells.

[0049] Furthermore, the cryopreservation of living cells includes the following steps: S1. Prepare a cryopreservation solution with a protective agent concentration of 2 times the final concentration and half the final volume, slowly add the cryopreservation solution to the culture medium along the wall of the tube, and place it in a 4°C environment for more than 10 minutes; S2. Take 10 μl of the cell suspension to be frozen and count, calculate the cell density and viability, and add a certain amount of preservation solution to the centrifuge tube based on the cell counting results so that the cell density in the tube is twice the freezing density; S3. Slowly add a volume of cell freezing solution equal to the volume of preservation solution added to the cell suspension along the wall of the tube, gently blow and mix, so that the cell density is consistent with the instruction requirements; S4. Dispense the cell suspension into cryopreservation tubes, 1 ml per tube; S5. Place the cells to be frozen in a freezing box filled with isopropanol, let it stand in a -80°C environment for a period of time, and then transfer it to a liquid nitrogen tank.

[0050] Furthermore, live cell cryopreservation also includes cryopreservation tube labeling and sampling testing.

[0051] Furthermore, the live cell recovery includes the following steps: S1. The temperature of the constant temperature water bath is adjusted to 37°C; S2. The cell cryopreservation tube is taken out from the liquid nitrogen tank and immediately placed in 37°C warm water and gently shaken until the cryopreservation solution is completely dissolved.

[0052] Furthermore, the freezing solution used in the living cell freezing step is 10% DMSO, 5% human serum albumin, 1% dextran and compound electrolyte injection.

[0053] Furthermore, the labels in the living cell freezing step include but are not limited to number information, cell generation information, cell freezing batch number information, freezing date information and operator information.

[0054] Furthermore, sampling and testing include but are not limited to microbial testing items and endotoxin testing items.

[0055] Furthermore, the freezing density in the living cell freezing step ranges from 0.5 to 2×10 8 / ml.

[0056] Furthermore, the cell aerosol is prepared by mixing physiological saline, 5% human serum albumin and cells.

[0057] Through in vitro cell culture, improvements to the in vitro culture medium for living cells can improve the cell activity problem after stem cell atomization.

[0058] Example 2

[0059] Please refer to Figures 2, 3, 4, 5, 6 and 7. A laboratory conducted animal experiments on aerosol inhalation of living cells;

[0060] Cell type: The cells tested were umbilical cord mesenchymal stem cells, which can be extended to any beneficial living cells;

[0061] Cell density: 1×10 8 / ml;

[0062] Cell aerosol composition: DMEM cell culture medium, 5% human serum albumin and cells;

[0063] Experimental animals: Wild-type mice;

[0064] Inhalation dose: 3-5 ml 1×10 8 / ml cell suspension, nebulized and inhaled within 15 minutes;

[0065] Cell labeling: Green fluorescent protein (GFP) labeling, in vivo animal imaging showed that large cells reside in the lungs;

[0066] Inhalation frequency and interval: Administer every other day for 5 consecutive times;

[0067] Results: Tissue section identification (secondary staining) showed 1 to 2 living umbilical cord mesenchymal stem cells in each 400x field of view.

[0068] Example 3

[0069] Referring to Figures 8 and 9, a live cell aerosol inhalation device includes a housing 2, a mounting assembly 1 disposed at the bottom of the housing 2, a connecting portion 3 and a transfer portion 4 disposed on one side of the housing 2, with the connecting portion 3 positioned directly above the transfer portion 4. An atomizing portion 5 is disposed on top of the connecting portion 3. The interior of the housing 2 includes a driving portion 7, an input portion 8, and an output portion 9, with the output portion 9 positioned near the transfer portion 4. The driving portion 7 is positioned between the input and output portions 8 and 9. The portable aerosol inhalation device allows aerosolized stem cells to be inhaled into the lungs, resolving the current pain point of clinical stem cell therapy requiring infusion.

[0070] Example 4

[0071] A living cell atomization inhalation device provided in Example 3 is further optimized. Specifically, as shown in Figures 9 and 10, a No. 1 partition 21 and a No. 2 partition 22 are fixedly installed inside the housing 2. The No. 1 partition 21 and the No. 2 partition 22 divide the interior of the housing 2 into a No. 1 area, a No. 2 area, and a No. 3 area. The driving unit 7 includes a No. 2 piston 71 slidably arranged inside the housing 2 and a No. 1 transmission rod 73 arranged in the No. 2 area. A pressing head 72 is provided above the housing 2. The top of the No. 1 transmission rod 73 passes through the housing 2 and is fixedly connected to the pressing head 72. The No. 1 transmission rod 73 is linked to the pressing head 72. The bottom end of the No. 1 transmission rod 73 is provided with a No. 2 transmission rod 74. The No. 1 transmission rod 73 is linked to the No. 2 transmission rod 74. The bottom end of the No. 2 transmission rod 74 passes through the No. 2 area and is fixedly connected to the No. 2 piston 71. The No. 2 transmission rod 74 is linked to the No. 2 piston 71. By controlling the pressing head 72 to perform linear reciprocating motion, the No. 2 piston 71 can be driven to perform linear displacement.

[0072] Furthermore, as shown in Figures 8, 9, 10 and 22, an air inlet is provided on the side of the outer shell 2 away from the transfer part 4, and a side cover 6 is detachably installed below the air inlet. The input part 8 includes a one-way valve 81 fixedly installed in the air inlet and a support rod 84 arranged in the No. 1 area. The bottom end of the support rod 84 passes through the No. 1 area and is threadedly connected to the No. 2 piston 71. The support rod 84 is linked with the No. 2 piston 71 and has the characteristic of easy disassembly and assembly. At least four inner rings 89 are detachably installed on the outer wall of the support rod 84. The support rod 84 is linked with the inner ring 89. A circular ring 85 is provided on the outer side of the inner ring 89, and a support bar 810 is provided between the inner ring 89 and the circular ring 85. The inner ring 89 is linked with the circular ring 85, and a filter net 88 is fixed in the circumferential gap between the inner ring 89 and the circular ring 85. The filter 88 can filter some impurities in the input air. During the linear displacement of the No. 2 piston 71, the ring 85 and the inner ring 89 move synchronously. The friction between the ring 85 and the inner wall of the No. 1 area generates static electricity, and the impurities that are not processed by the filter 88 are fixed by electrostatic adsorption.

[0073] Furthermore, as shown in Figures 9, 10, 15 and 16, an exhaust port is provided on one side of the housing 2 near the transfer portion 4, and the output portion 9 includes a drive rod 91, a rectangular frame 92, a transmission disk 94, a sleeve 910, a telescopic rod 911, a linkage rod 912 and a generator arranged in the No. 3 area. The bottom end of the drive rod 91 passes through the third area and is fixedly connected to the No. 2 piston 71. The drive rod 91 is linked to the No. 2 piston 71. The top of the drive rod 91 is fixedly connected to the rectangular frame 92. The drive rod 91 is linked to the rectangular frame 92. The bottom end of the telescopic rod 911 is slidably arranged inside the sleeve 910. The bottom end of the sleeve 910 is fixedly connected to the rectangular frame 92. The sleeve 910 is linked to the rectangular frame 92. The two ends of the linkage rod 912 are respectively rotatably connected to the telescopic rod 911 and the transmission disk 94. The connection point between the linkage rod 912 and the transmission disk 94 deviates from the center point of the transmission disk 94, thereby converting linear motion into rotary motion. The transmission disk 94 is fixedly mounted on the drive shaft of the generator. By converting the linear motion into rotary motion, the transmission plate 94 is driven to rotate, so that the generator generates electrical energy, which is stored in the battery installed above the generator to provide electrical energy for the power-demanding units.

[0074] Furthermore, as shown in Figures 9 and 20, the transfer part 4 includes a tank body 41, a hose 42 is provided inside the tank body 41, and an end cover 43 is detachably installed on the top of the tank body 41. A limiting hole is provided on the top of the end cover 43, and an output head 45 is slidably provided in the limiting hole. The output head 45 can passively perform telescopic movement, and a No. 4 spring 44 is provided below the output head 45. The setting of the No. 4 spring 44 enables the output head 45 to automatically reset after the force controlling the movement disappears. The top end of the hose 42 passes through the end cover 43, the No. 4 spring 44 and is slidably connected to the output head 45. The outer wall of the tank body 41 is connected to the air supply pipe, and the air supply pipe is inserted into the exhaust port. When the No. 2 piston 71 performs linear reciprocating motion, including upward and downward motion, when moving downward, the air enters the No. 1 area, the No. 2 area and the No. 3 area through the one-way valve 81. When moving upward, the air cannot be discharged at the one-way valve 81, and thus enters the interior of the tank body 41 through the air pipe, pressurizing the interior of the tank body 41, and the liquid inside the tank body 41 moves upward through the hose 42 and the output head 45.

[0075] Furthermore, as shown in Figures 8, 9, 20 and 21, the communication portion 3 includes a communication platform 31 fixedly mounted on one side of the housing 2, an output channel 33 and a limit slide are provided inside the communication platform 31, a pressure conversion assembly 34 is provided inside the output channel 33, a press switch 32 is provided in the limit slide, and the press switch 32 is subjected to a pressing force and can slide passively. A No. 3 spring 35 is fixedly mounted on the press switch 32. The setting of the No. 3 spring 35 enables the press switch 32 to automatically reset after the force controlling the movement disappears. One end of the press switch 32 extends through the limit slide to the output When the push switch 32 is pressed once, the elastic buckle 36 is stuck in the slot 37, and the No. 3 spring 35 is forced to shrink. When the push switch 32 is pressed a second time, the elastic buckle 36 withdraws from the slot 37 and continues to move forward. After the force controlling the push switch 32 disappears, the No. 3 spring 35 rebounds quickly, and the reset speed is much greater than the speed of one press. During the retreat process, the elastic buckle 36 passes over the slot 37, thereby resetting the push switch 32.

[0076] By pressing the switch 32 to control the pressure conversion component 34, the function of quantitative liquid delivery is realized, the delivery amount of the liquid is accurately controlled, and the waste caused by excessive or insufficient use of the liquid can be reduced. The operation is simple and suitable for personal operation.

[0077] Furthermore, as shown in Figures 20 and 21, the pressure conversion assembly 34 includes a shell 341 fixedly installed inside the output channel 33, an input port 343 is provided at the bottom of the shell 341, an output port 344 is provided at the top of the shell 341, and a sliding switch 345 is provided inside the shell 341 for sliding. Liquid enters the output channel 33 through the output head 45, pushing the sliding switch 345 to slide. One side of the sliding switch 345 is fixedly connected to the inner wall of one side of the shell 341 with a No. 2 spring 342. The setting of the No. 2 spring 342 controls the sliding displacement of the sliding switch 345 so that it can automatically move after the force of the sliding displacement disappears. A groove is provided on the other side of the sliding switch 345, and a through hole is provided in the middle of the sliding switch 345. A limit rod 346 is fixedly connected to the inner wall of the other side of the shell 341 to limit the range of movement of the sliding switch 345, and a through hole is also provided in the middle of this side to correspond to the press switch 32. When the sliding switch 345 is not subjected to fluid pressure, a small portion of the output port 344 is still closed. The diameter of the through hole is smaller than that of the input port 343. Liquid enters through the input port 343 and encounters a certain resistance when passing through the through hole, thereby generating a thrust on the sliding switch 345. When the liquid pressure is high, the thrust on the sliding switch 345 is large, and when the liquid pressure is low, the thrust on the sliding switch 345 is small. The greater the displacement distance of the sliding switch 345, the more area of ​​the output port 344 is closed, and the smaller the displacement distance of the sliding switch 345, the smaller the area of ​​the output port 344 is closed, thereby reducing the uneven liquid pressure and maintaining a stable output.

[0078] Furthermore, as shown in Figures 8 and 9, the atomizing unit 5 includes an atomizer 51 fixedly mounted on the top of the connecting platform 31. A hole communicating with the output channel 33 is provided at the bottom of the atomizer 51. The liquid enters the interior of the atomizer 51 through the output port 344. A connector 52 is fixedly connected to the outer wall of the atomizer 51. A filter mesh 54 is fixedly provided inside the connector 52. An atomizing sheet is provided inside the atomizer 51. The atomizing sheet inside the atomizer 51 atomizes the liquid, and the filter mesh 54 filters the atomized liquid again. The liquid can be atomized and filtered again, and the connector 52 can be docked with the mask for easy inhalation. The atomizing aperture of the atomizer (i.e., the aperture of a single atomizing hole) is: aperture 5-100um, and the atomization rate is 0.8ml / min-20ml / min; calculated based on 1500 atomizing holes, the atomization rate of a single hole is 0.53μL / min-13.4μL / min;.

[0079] Example 5

[0080] A living cell atomization inhalation device provided in Example 3 or 4 is further optimized. Specifically, as shown in Figures 8, 9, 17, 18 and 19, the mounting assembly 1 includes a base 11 fixedly mounted on the bottom of the housing 2. The interior of the base 11 is provided with a first cavity 15, a second cavity 16, a third cavity 18, a fourth cavity 110 and a fifth cavity 112, and the third cavity 18 is connected to the first cavity 15 and the second cavity 16. The top of the base 11 is provided with a first connecting hole 17 connected to the second cavity 16, a third connecting hole 111 is provided between the second cavity 16, the first cavity 15 and the fourth cavity 110, and the interior of the first cavity 15 is provided with a negative pressure. The fixing component 13, the negative pressure fixing component 13 includes a threaded cylinder 138 fixedly arranged inside the No. 1 cavity 15 and a No. 1 piston 135 slidably arranged inside the No. 3 cavity 18. The No. 1 piston 135 can slide and displace in the No. 3 cavity 18. The internal thread of the threaded cylinder 138 is installed with a cylinder 131. The top of the cylinder 131 is fixedly installed with a winding wheel 132. The bottom of the cylinder 131 is fixedly installed with a locking hook 137. A No. 1 spring 134 is fixedly connected between one side of the No. 1 piston 135 and the inner wall of one side of the No. 3 cavity 18. The No. 1 spring 134 is set so that the No. 1 piston 135 can automatically reset after the force of the sliding displacement disappears. The other side of the No. 1 piston 135 is fixedly connected with a pull rope 136. The end of the pull rope 136 passes through the No. 3 cavity 18 and is fixedly connected to the winding wheel 132. A No. 2 connecting hole 19 is opened on the top of the base 11. When the No. 2 piston 71 moves downward, a downward force is applied to the winding wheel 132, causing the cylinder 131 to rotate and move downward. During the rotation of the winding wheel 132, the pull rope 136 is wound. When the pull rope 136 is rolled up, it pulls the No. 1 piston 135 to move, forming a negative pressure environment in the No. 2 cavity 16. When the No. 1 connecting hole 17 is closed at the bottom of the tank body 41, the tank body 41 is automatically fixed by the negative pressure.

[0081] Furthermore, as shown in Figures 8, 9, 17, 18 and 19, an exhaust hole 14 communicating with cavity No. 1 15 is provided on one side of the base 11. When piston No. 2 71 moves downward, the air in cavity No. 1 15 is discharged through the exhaust hole 14. A threaded hole communicating with cavity No. 5 112 is provided on the other side of the base 11. A switch assembly 12 is provided on one side of the base 11. The switch assembly 12 includes a threaded rod 122 threadedly installed in the threaded hole. One end of the threaded rod 122 is fixedly connected to a knob 121, the other end of the threaded rod 122 is fixedly connected to an extension rod, and the end of the extension rod is fixedly connected to a sealing plug 124. A limited position channel is opened between cavity No. 5 112 and cavity No. 2 16. By controlling the threaded rod 122 to rotate through the knob 121, the threaded rod 122 can move linearly and drive the sealing plug 124 to move synchronously. The sealing plug 124 exits the limiting channel, and the No. 2 cavity 16 can be connected with the outside world, the negative pressure environment returns to normal, and the disassembly of the tank body 41 is completed.

[0082] Further, as shown in Figures 9, 17, 18 and 19, a No. 4 connecting hole is provided between the No. 4 cavity 110 and the No. 5 cavity 112, a No. 3 connecting hole 111 is provided between the No. 4 cavity 110 and the No. 1 cavity 15, and a movable rod 126 is provided inside the No. 4 cavity 110. A support ring 127 is fixedly sleeved on the movable rod 126, and a rotating ring 123 is movably sleeved on the extension rod. The extension rod is linked to the threaded rod 122, and the extension rod can move synchronously with the rotating ring 123. The outer wall of the rotating ring 123 is fixedly connected to the No. 1 connecting rod. Rod 125, the bottom end of the No. 1 connecting rod 125 passes through the No. 4 connecting hole and is fixedly connected to one end of the movable rod 126, the No. 1 connecting rod 125 is linked with the rotating ring 123 and the movable rod 126, the end of the movable rod 126 is fixedly connected with the No. 2 connecting rod 128, the movable rod 126 is linked with the No. 2 connecting rod 128, the top of the No. 2 connecting rod 128 is fixedly connected with the mounting seat 139, the mounting seat 139 is located inside the No. 3 connecting hole 111, and one end of the mounting seat 139 is rotatably connected to the locking rod 1310, and a No. 1 torsion spring is provided at the connection. When the cylinder 131 moves downward, it drives the locking hook 137 to move synchronously, and the locking hook 137 pushes the locking rod 1310 to rotate counterclockwise. The locking hook 137 continues to move downward, and the No. 1 torsion spring automatically resets the locking rod 1310. The locking hook 137 and the locking rod 1310 are stuck, so that the cylinder 131 maintains the current position, and the threaded rod 122 moves linearly, driving the sealing plug 124 to move synchronously. When the sealing plug 124 exits the limiting channel, the locking rod 1310 is driven to move away from the locking hook 137 through the rotating ring 123, the No. 1 connecting rod 125, the movable rod 126, the No. 2 connecting rod 128 and the mounting seat 139, thereby releasing the lock.

[0083] Example 6

[0084] A living cell atomization inhalation device provided in Examples 3, 4 or 5 is further optimized. Specifically, as shown in Figures 9, 10, 11 and 14, a transmission switching assembly 75 is provided inside the No. 1 transmission rod 73, a special-shaped hole is provided on the No. 1 partition 21, and a moving assembly 76 is provided in the special-shaped hole. The moving assembly 76 includes a moving platform 761 that is slidably arranged in the special-shaped hole, a No. 6 spring 763 and a telescopic block 764 are provided inside the moving platform 761, and the telescopic block 764 is located above the No. 6 spring 763. A through-hole is provided on the top of the moving platform 761, and a No. 1 drive block 765 and a No. 2 drive block 766 are fixed on both sides of the moving platform 761 respectively. The edge of the No. 1 drive block 765 is a double bevel, and the edge of the No. 2 drive block 766 is an arc surface. The top of the special-shaped hole is a bevel, and holes are provided on both sides of the special-shaped hole. Part of the structure of the No. 1 drive block 765 is located in the No. 1 area. When the No. 2 piston 71 performs linear reciprocating motion, it drives the ring 85 to move synchronously. The ring 85 repeatedly contacts the inclined surface of the No. 1 drive block 765, exerting a push on the No. 1 drive block 765, thereby pushing the movable platform 761 and the No. 2 drive block 766 toward the direction of the No. 2 area. The faster the movement speed of the No. 2 piston 71, the longer the No. 2 drive block 766 stays in the No. 2 area.

[0085] Furthermore, in Figures 9, 10, 11, 12 and 13, the transmission switching assembly 75 includes a push rod 751 rotatably mounted inside the No. 1 transmission rod 73, and a No. 2 torsion spring is provided at the connection point. A No. 1 slider 753 is slidably mounted inside the No. 1 transmission rod 73, a connecting rod 752 is provided between the push rod 751 and the No. 1 slider 753, and the two ends of the connecting rod 752 are rotatably connected to the push rod 751 and the No. 1 slider 753 respectively. A movable bar 754 is fixedly connected to the bottom of the No. 1 slider 753, and a slide groove 756 is provided on the rear side of the movable bar 754. The inner portion of the slide groove 756 is provided with a plurality of movable bars. A No. 2 slider 757 is provided for sliding movement, a mounting tube 755 is fixedly installed inside the No. 1 transmission rod 73, two limiting rings 759 are fixedly provided inside the mounting tube 755, and two No. 1 ball head buckles 758 are provided for sliding movement inside the mounting tube 755, a No. 5 spring 7510 is fixedly connected between the No. 1 ball head buckle 758 and the limiting ring 759, a connecting rope 7511 is fixedly connected to the bottom of the No. 2 slider 757, and the ends of the connecting rope 7511 are fixedly connected to the two No. 1 ball head buckles 758 through two connecting belts, and two card holes are provided on the No. 2 transmission rod 74.

[0086] After the first gear 73 is in the state of being moved back and forth in a straight line, the first gear 73 is in the state of being moved back and forth in a straight line. When the first gear 73 is displaced upward, it drives the push rod 751, which contacts the second gear 766 and rotates counterclockwise, causing the first slider 753 to slide downward through the connecting rod 752, and the movable bar 754 is displaced downward synchronously and staggered with the mounting tube 755. When the first gear 73 is displaced downward, it drives the push rod 751, which contacts the second gear 766 and rotates clockwise, causing the first slider 753 to slide upward through the connecting rod 752, and the movable bar 754 is displaced upward synchronously, and the connecting rope 7511 is pulled by the second slider 757. The connecting rope 7511 pulls the No. 1 ball head buckle 758 toward the middle of the mounting tube 755, so that the No. 1 gear 73 is decoupled from the No. 1 gear 73, which can automatically cut off the power transmission and improve the operation stability.

[0087] Furthermore, as shown in Figures 9, 10, 22, 23 and 24, a mounting groove is provided on the top of the support bar 810, and a No. 2 ball head buckle 811 is slidably provided in the mounting groove and a No. 7 spring 812 is fixedly installed thereon. A pull rod 813 is also provided in the mounting groove, one end of the pull rod 813 passes through the No. 7 spring 812 and is fixedly connected to the support bar 810, and the other end of the pull rod 813 is fixedly connected to the operating block 814. A blind hole is provided on the inner wall of the ring 85, a notch is provided on the outer wall of the ring 85, and a No. 4 slider 86 is rotatably installed in the notch. A No. 5 slider 87 is also fixedly provided on the outer wall of the ring 85, and a No. 1 area is provided with a No. 4 slider 86 and a No. 5 slider 87. The top of the housing 2 has a mounting hole that communicates with the No. 1 area, with a top cover 815 threadedly mounted within the mounting hole. A knob post 82 is fixed to the top of the top cover 815, and its outer wall is provided with anti-slip grooves. A hollow rod 83 is fixed to the bottom of the top cover 815, and a collar with a circular hole is slidably mounted on the hollow rod 83. The collar is then disassembled by rotating the knob post 82, which can be inserted into the exhaust port. The second ball head clip 811 is then slid out of the blind hole by operating the operating block 814 and the pull rod 813, allowing the inner ring 89 to be separated from the circular ring 85.

[0088] Furthermore, as shown in FIG25 , a stabilizing rod 816 is provided inside the support rod 84. A No. 8 spring 817 is fixedly provided inside the stabilizing rod 816. A No. 3 ball head buckle 818 is fixedly provided at one end of the No. 8 spring 817. Both the support rod 84 and the stabilizing rod 816 have mutually aligned through holes. The inner wall of the inner ring 89 also has a bayonet. The end of the No. 3 ball head buckle 818 passes through the through hole and is secured into the bayonet. To remove the side cover 6, the support rod 84 can be rotated to be removed. Pulling the stabilizing rod 816 causes the No. 3 ball head buckle 818 to exit the bayonet due to the tension, and the stabilizing rod 816 is separated from the support rod 84. Simultaneously, the support rod 84 is separated from the inner ring 89.

[0089] Furthermore, as shown in Figures 15 and 16, a movable frame 96 is slidingly provided inside the rectangular frame 92, and racks 97 are fixedly provided on the inner walls on both sides of the movable frame 96. Strip-shaped through holes 98 are opened on both sides of the rectangular frame 92. Pressure heads 99 are fixedly connected to both sides of the movable frame 96, and the two pressure heads 99 extend to the outside of the rectangular frame 92 through the two strip-shaped through holes 98 respectively. Triangular block No. 1 93 and triangular block No. 2 95 are fixedly installed in area No. 3, and triangular block No. 1 93 and triangular block No. 2 95 are respectively located on both sides of the rectangular frame 92. The tank body 41 is disassembled, and the knob column 82 is inserted into the exhaust port. The anti-slip groove on the outer wall of the knob column 82 is engaged with the rack 97. The support rod 84 and the stabilizing rod 816 are respectively inserted into the No. 1 connecting hole 17 and the output channel 33. After the inner ring 89 of one group is separated from the support rod 84, the No. 4 slider 86 is threadedly installed on the end of the support rod 84 with an internal thread. The No. 5 slider 87 is inserted into the end of the stabilizing rod 816. The end of the hollow rod 83 is inserted into the inner ring 89 to control the sliding of the collar. The part of the collar without a circular hole is filled in the circumferential gap between the hollow rod 83 and the inner ring 89 to support and limit the hollow rod 83. The remaining groups of inner rings 89 are separated from the circular ring 85. The No. 4 sliders 86 on the outer walls of the groups of circular rings 85 are screwed. The groove is installed in the circular hole on the ring. When the No. 2 piston 71 moves, the rectangular frame 92 is driven to reciprocate linearly through the driving rod 91, and the movable frame 96 is driven to move synchronously. The installation positions of the two pressure heads 99 are respectively close to the top and bottom of the side of the movable frame 96. The installation position of the No. 1 triangle block 93 is at the same height as the bottom of the rectangular frame 92, and the inclined surface is facing upward. The installation position of the No. 2 triangle block 95 is at the same height as the top of the rectangular frame 92, and the inclined surface is facing downward. When the movable frame 96 reciprocates linearly, the movable frame 96 performs linear reciprocating motion in the vertical direction and the horizontal direction, which can drive the knob column 82 and the hollow rod 83 to rotate. The overall structure is horizontal, and the centrifuge tube is inserted into the ring 85, which can be subjected to a centrifugal step.

[0090] The use process of a living cell atomization inhalation device and a method of use provided by the present invention is as follows: living cells that need to be atomized and inhaled are cultured in vitro, the living cells cultured in vitro are subjected to relevant quality inspections, specific living cell markers are identified, the living cells cultured in vitro are centrifuged to discard the supernatant, and then a certain amount of living cell culture fluid and lung cell protective fluid are added for resuspending, the living cells are frozen, the living cells are revived, a cell atomizer is prepared, and continuous atomization is performed through the atomization device. During the centrifugal operation, the drive part 7, the input part 8 and the output part 9 cooperate, and the function of centrifugal separation is achieved through structural change assembly, which can be applied to cell centrifugation work. During the atomization operation, the drive part 7, the output part 9, the transfer part 4 and the connecting part 3 cooperate to evenly input the physiological saline mixed with stem cells into the interior of the nebulizer 51. The connector 52 can be connected to the mask, and after atomization, it is output through the connector 52 for inhalation.

[0091] Obviously, the embodiments described above are only some embodiments of the present invention, rather than all embodiments. The preferred embodiments of the present invention are shown in the accompanying drawings, but they do not limit the scope of the present invention. The present invention can be implemented in many different forms. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure of the present invention more thorough and comprehensive. Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned specific embodiments, or to replace some of the technical features therein with equivalents.

Claims

1. A living cell atomization inhalation device, characterized in that: The invention comprises a shell (2), wherein a mounting assembly (1) is provided at the bottom of the shell (2), a connecting portion (3) and a transfer portion (4) are provided at one side of the shell (2), and the connecting portion (3) is located directly above the transfer portion (4), and an atomizing portion (5) is provided at the top of the connecting portion (3); a driving portion (7), an input portion (8) and an output portion (9) are provided inside the shell (2), and the output portion (9) is located at a side close to the transfer portion (4), and the driving portion (7) is located between the input portion (8) and the output portion (9); a first partition plate (21) and a second partition plate (22) are fixedly installed inside the shell (2), The first partition plate (21) and the second partition plate (22) divide the interior of the housing (2) into a first area, a second area and a third area. The driving unit (7) comprises a second piston (71) slidably arranged inside the housing (2) and a first transmission rod (73) arranged in the second area. A pressing head (72) is arranged above the housing (2). The top end of the first transmission rod (73) passes through the housing (2) and is fixedly connected to the pressing head (72). A second transmission rod (74) is arranged at the bottom end of the first transmission rod (73). The bottom end of the second transmission rod (74) passes through the second area and is fixedly connected to the second piston (71). The housing (2) is provided with an air inlet on one side away from the transfer part (4), and a side cover (6) is detachably mounted below the air inlet. The input part (8) comprises a one-way valve (81) fixedly mounted in the air inlet and a support rod (84) arranged in the No. 1 area. The bottom end of the support rod (84) passes through the No. 1 area and is threadedly connected to the No. 2 piston (71). At least four inner rings (89) are detachably mounted on the outer wall of the support rod (84). A circular ring (85) is disposed on the outer side of the inner ring (89), and a support strip (810) is disposed between the inner ring (89) and the circular ring (85). A filter screen (88) is fixedly arranged in the circumferential gap between the outer shell (2) and the circular ring (85); an exhaust port is opened on one side of the outer shell (2) close to the transfer part (4); the output part (9) comprises a driving rod (91), a rectangular frame (92), a transmission plate (94), a sleeve (910), a telescopic rod (911), a linkage rod (912) and a generator arranged in the third area; the bottom end of the driving rod (91) passes through the third area and is fixedly connected to the second piston (71); the top end of the driving rod (91) is fixedly connected to the rectangular frame (92); the bottom end of the telescopic rod (911) is slidably arranged inside the sleeve (910); the bottom end of the sleeve (910) is fixedly connected to the rectangular frame (92); the two ends of the linkage rod (912) are rotatably connected to the telescopic rod (911) and the transmission plate (94) respectively; the transmission plate (94) is fixedly installed on the transmission shaft of the generator;The transfer part (4) comprises a tank body (41), a hose (42) is arranged inside the tank body (41), an end cover (43) is detachably mounted on the top of the tank body (41), a limiting hole is arranged on the top of the end cover (43), and an output head (45) is slidably arranged in the limiting hole, a No. 4 spring (44) is arranged below the output head (45), the top end of the hose (42) passes through the end cover (43), the No. 4 spring (44) and is slidably connected to the output head (45), the outer wall of the tank body (41) is connected to an air delivery pipe, and the air delivery pipe is inserted into the exhaust pipe. The connecting portion (3) comprises a connecting platform (31) fixedly mounted on one side of the housing (2); an output channel (33) and a limit slide are provided inside the connecting platform (31); a pressure conversion assembly (34) is provided inside the output channel (33); a push switch (32) is slidably provided inside the limit slide; a No. 3 spring (35) is fixedly mounted on the push switch (32); one end of the push switch (32) passes through the limit slide and extends into the output channel (33); a card slot (37) is provided inside the limit slide; and the push switch (32) is provided with a card slot (37). The pressure switch (32) is fixedly provided with an elastic buckle (36); the pressure conversion assembly (34) comprises a shell (341) fixedly installed inside the output channel (33); the bottom of the shell (341) is provided with an input port (343); the top of the shell (341) is provided with an output port (344); a sliding switch (345) is slidably provided inside the shell (341); one side of the sliding switch (345) is fixedly connected to the inner wall of one side of the shell (341) with a No. 2 spring (342); the sliding switch (345) is A groove is provided on the other side, a through hole is provided in the middle of the sliding switch (345), and a limit rod (346) is fixedly connected to the inner wall of the other side of the housing (341); the atomizing portion (5) comprises an atomizer (51) fixedly mounted on the top of the connecting platform (31), a hole communicating with the output channel (33) is provided at the bottom of the atomizer (51), a connector (52) is fixedly connected to the outer wall of the atomizer (51), a filter mesh (54) is fixedly provided inside the connector (52), and an atomizing sheet is provided inside the atomizer (51);The mounting assembly (1) comprises a base (11) fixedly mounted on the bottom of the housing (2); a first cavity (15), a second cavity (16), a third cavity (18), a fourth cavity (110) and a fifth cavity (112) are provided inside the base (11); the third cavity (18) is communicated with the first cavity (15) and the second cavity (16); a first connecting hole (17) is provided on the top of the base (11) and is communicated with the second cavity (16); the first cavity (15) and the fourth cavity (110) are connected to each other; A No. 3 communicating hole (111) is provided between the No. 1 cavity (15), a negative pressure fixing assembly (13) is provided inside the No. 1 cavity (15), and the negative pressure fixing assembly (13) comprises a threaded cylinder (138) fixedly arranged inside the No. 1 cavity (15) and a No. 1 piston (135) slidably arranged inside the No. 3 cavity (18), a cylinder (131) is installed with internal threads of the threaded cylinder (138), a winding wheel (132) is fixedly installed on the top of the cylinder (131), and a locking hook (132) is fixedly installed on the bottom of the cylinder (131). 137), a No. 1 spring (134) is fixedly connected between one side of the No. 1 piston (135) and the inner wall of one side of the No. 3 cavity (18), a pull rope (136) is fixedly connected to the other side of the No. 1 piston (135), the end of the pull rope (136) passes through the No. 3 cavity (18) and is fixedly connected to the winding wheel (132), a No. 2 connecting hole (19) is opened on the top of the base (11); a vent hole (14) communicating with the No. 1 cavity (15) is opened on one side of the base (11), and the base (11) is provided with a plurality of connecting holes (19) and a plurality of connecting holes (19) connected to the No. 1 cavity (15). 1) is provided with a threaded hole communicating with the fifth cavity (112), one side of the base (11) is provided with a switch assembly (12), the switch assembly (12) comprises a threaded rod (122) threadedly installed in the threaded hole, one end of the threaded rod (122) is fixedly connected to a knob (121), the other end of the threaded rod (122) is fixedly connected to an extension rod, and the end of the extension rod is fixedly connected to a sealing plug (124), and a limited position channel is provided between the fifth cavity (112) and the second cavity (16);A No. 4 communication hole is provided between the No. 4 cavity (110) and the No. 5 cavity (112), a No. 3 communication hole (111) is provided between the No. 4 cavity (110) and the No. 1 cavity (15), a movable rod (126) is provided inside the No. 4 cavity (110), a support ring (127) is fixedly sleeved on the movable rod (126), a rotating ring (123) is movably sleeved on the extension rod, a No. 1 connecting rod (125) is fixedly connected to the outer wall of the rotating ring (123), the bottom end of the No. 1 connecting rod (125) passes through the No. 4 communication hole and is fixedly connected to one end of the movable rod (126), the end of the movable rod (126) is fixedly connected to the No. 2 connecting rod (128), and the No. 2 connecting rod (128) is fixedly connected to the end of the movable rod (126). The top of the mounting seat (139) is fixedly connected to the No. 3 connecting hole (111), and the mounting seat (139) is located inside the No. 3 connecting hole (111). One end of the mounting seat (139) is rotatably connected to a locking rod (1310), and a No. 1 torsion spring is provided at the connection point; a transmission switching component (75) is provided inside the No. 1 transmission rod (73), and a special-shaped hole is provided on the No. 1 partition plate (21), and a moving component (76) is provided in the special-shaped hole. The moving component (76) includes a moving platform (761) slidably arranged in the special-shaped hole, and a No. 6 spring (763) and a telescopic block (764) are provided inside the moving platform (761), and the telescopic block (764) is located above the No. 6 spring (763). The top of the moving platform (761) A through hole is provided on the part, and a No. 1 driving block (765) and a No. 2 driving block (766) are fixedly provided on both sides of the moving platform (761); the transmission switching assembly (75) comprises a push rod (751) rotatably installed inside the No. 1 transmission rod (73), and a No. 2 torsion spring is provided at the connection point; a No. 1 slider (753) is slidably installed inside the No. 1 transmission rod (73); a connecting rod (752) is provided between the push rod (751) and the No. 1 slider (753), and the two ends of the connecting rod (752) are rotatably connected to the push rod (751) and the No. 1 slider (753), respectively; a movable bar (754) is fixedly connected to the bottom of the No. 1 slider (753), and a sliding groove (756) is provided on the rear side of the movable bar (754). ), a second slider (757) is slidably provided inside the slide groove (756), a mounting tube (755) is fixedly installed inside the first transmission rod (73), two limiting rings (759) are fixedly provided inside the mounting tube (755), and two first ball head buckles (758) are slidably provided inside the mounting tube (755), a fifth spring (7510) is fixedly connected between the first ball head buckle (758) and the limiting ring (759), a connecting rope (7511) is fixedly connected to the bottom of the second slider (757), and the ends of the connecting rope (7511) are respectively fixedly connected to the two first ball head buckles (758) through two connecting belts, and two clamping holes are provided on the second transmission rod (74);The support bar (810) is provided with an installation groove at the top, and a No. 2 ball head buckle (811) is slidably provided in the installation groove and a No. 7 spring (812) is fixedly installed. A pull rod (813) is also provided in the installation groove, and one end of the pull rod (813) passes through the No. 7 spring (812) and is fixedly connected to the support bar (810), and the other end of the pull rod (813) is fixedly connected to an operating block (814). A blind hole is provided on the inner wall of the ring (85), and a notch is provided on the outer wall of the ring (85), and a No. 4 slider (86) is rotatably installed in the notch. A No. 5 slider (87) is also fixedly provided on the outer wall of the ring (85). A guide groove matching the No. 4 slider (86) and the No. 5 slider (87) is provided in the No. 1 area, and a guide groove matching the No. 1 area is provided on the top of the shell (2). The top cover (815) is provided with a mounting hole connected thereto, and a top cover (815) is installed with a thread in the mounting hole, a knob column (82) is fixedly provided on the top of the top cover (815), an anti-slip tooth groove is provided on the outer wall of the knob column (82), a hollow rod (83) is fixedly provided on the bottom of the top cover (815), a sleeve is slidably mounted on the hollow rod (83), and a round hole is provided on the sleeve; a stabilizing rod (816) is provided inside the support rod (84), and eight A No. 8 spring (817) is provided, and a No. 3 ball head buckle (818) is fixedly provided at one end of the No. 8 spring (817). Through holes that are aligned with each other are provided on the support rod (84) and the stabilizing rod (816). A bayonet is also provided on the inner wall of the inner ring (89). The end of the No. 3 ball head buckle (818) passes through the through hole and is mounted in the bayonet. A movable frame (96) is slidably provided inside the rectangular frame (92). Racks are fixedly provided on the inner walls of both sides of the movable frame (96). (97), strip-shaped through holes (98) are provided on both sides of the rectangular frame (92), pressure bearing heads (99) are fixedly connected to both sides of the movable frame (96), and the two pressure bearing heads (99) respectively pass through the two strip-shaped through holes (98) and extend to the outside of the rectangular frame (92), and a number one triangular block (93) and a number two triangular block (95) are fixedly installed in the number three area, and the number one triangular block (93) and the number two triangular block (95) are respectively located on both sides of the rectangular frame (92). ;

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