COMPOSITION FOR BIOSTIMULATION

Abstract

Composition for the biostimulation of plant growth comprising a bacterial active agent, said bacterial active agent comprising a bacterium of the genus Bacillus and a nitrogen-fixing bacterium, characterized in that the quantity of said bacterium of the genus Bacillus and of said nitrogen-fixing bacterium in said bacterial active agent is greater than or equal to 75% by weight relative to the total weight of said bacterial active agent in the composition.

Description

BE2025 / 5012 2 environmental and improve plant productivity. Biostimulant compositions can include different components such as microorganisms, vitamins, proteins, plant hormones, etc. In this context, document AU2021102872 describes a solid and liquid composition (agro-nutritive solid and liquid compost) and a process for its production comprising microorganisms and nutrients to promote plant growth. The agro-nutritive solid and liquid compost includes a biofertilizer such as Bacillus mylolique facilis, Pseudomonas fluorescens, Pseudomonas putida, Bacillus subtilis, Azospirillum brasilense and poultry manure for plant growth. This document describes multi-10 compositions microbials comprising at least four different microorganisms in the form of a microbial consortium. Document US20230159405 concerns an organic fertilizer for biofertilization and stimulation of plant growth.The biological fertilizer described in the document comprises a pure culture of an AO512 strain of Azospirillum oryzae, a pure culture of an AL20 strain of Azospirillum lipoferum, a pure culture of a PD2 strain of Pantoea dispersea, a pure culture of a TU2 strain of Bacillus subtilis, a pure culture of an S3 strain of Lysinibacillus phaericus, and a pure culture of an SR17 strain of Paenibacillus polymyxa. Although these documents tout the merits of their technology, and the compositions described in these documents may be of some interest for stimulating plant growth and fitting into a concept of sustainable crop development, the compositions described above present many drawbacks. In particular, the biostimulant compositions of the anterior art tend to combine a high number of different microorganisms, possibly with a high number of decofactors and nutrients, in order to increase the synergies and the spectrum of action of the different microorganisms on plant growth.Cescompositions biostimulantessontcomplexesetfastidieusesàproduire,lesrendantdifficilement industrialisables(donccouteuses):ellesnécessientlapréparationd'unmélanged nombreusesbactérie,quisontcommunémentproduites / fermentéesséparément.De30plus,lemélanged'unmbreimportantdemicro-organismesdiférents,ayantchacundesrésistancesdifférentesauxconditionsdestockages,auxconditions environmentallorsdel'administrationauxplantes,peutrendrecescompositions biostimulantespeustables,voireinefficaceset / ouayantuneffetbiostimulantpeu 2025 / 5012 BE2025 / 5012 3 reproduciblesilaproportiondesdifférentsmicro-organismes danscescompositions n'estpascorrectementdoséeet / ousilesconditionsdestockageet / oulesconditions enviromentaleslorsdel'administrationàlaplantenesontpasoptimales. There is therefore still a need to have an easily industrializable and stable composition, while allowing for an effective and reproducible biostimulation treatment5 with an important spectrum of action on plant growth.Objectives of the Invention The present invention aims to overcome the drawbacks of the prior art, in particular those described above.10 In particular, the present invention proposes to provide a simple and easily industrializable composition for carrying out an effective biostimulant treatment having a broad spectrum of action on the growth of different types of plants. The present invention also proposes to provide a stable composition allowing for a highly reproducible and15 controlled biostimulant treatment from one production batch to another and / or according to changing environmental conditions.Summary of the Invention To achieve these objectives, the present invention provides a composition for the biostimulation of plant growth comprising a bacterial active agent, said bacterial active agent comprising a bacterium of the genus Bacillus and a nitrogen-fixing bacterium, characterized in that the quantity of said bacterium of the genus Bacillus and said nitrogen-fixing bacterium in said bacterial active agent is greater than or equal to 75% by weight relative to the total weight of said bacterial active agent in the composition. The inventors have indeed discovered, surprisingly, that a composition for the biostimulation of plant growth with a simplified bacterial active agent comprising, in large part, specifically a bacterium of the genus Bacillus and a nitrogen-fixing bacterium provides excellent efficacy. biostimulation while allowing a very important simplification of the bacterial formulation and the composition formulation.2025 / 5012 BE2025 / 5012 4 The composition for the biostimulation of plant growth of the invention is therefore very easily industrialized because it only requires the production of two main types of bacteria. Moreover, and particularly surprisingly, with a "simple" bacterial active agent, this composition is particularly effective in ensuring an optimal biostimulant effect on the growth of 5 different types of plants (under a variety of growing conditions and environmental stresses). Furthermore, as mentioned, this composition is particularly stable, allowing for a highly reproducible and controlled biostimulant treatment from one production batch to another and / or according to changing environmental conditions, compared with the case of a complex mixture of many different bacteria, the balance of which is difficult to obtain and clearly plusinstableàmainteniret / ouàcontrôle.Other embodiments of the composition for the biostimulation of plant growth according to the present invention are indicated in the attached claims. 15 The present invention also relates to a seed of a plant of interest coated with the composition for the biostimulation of plant growth according to the present invention. Other embodiments of the seed of a plant of interest according to the present invention are indicated in the attached claims. 20 The present invention also relates to a method for increasing plant growth comprising the step of administering to said plants the composition according to the present invention, or the seed coated according to the present invention.The present invention also relates to the use of a composition to increase the growth of a plant (particularly under conditions of nitrogen and / or phosphorus deficiency, and / or as at least a partial replacement for nitrogen and / or phosphorus fertilization), said composition comprising a bacterial active agent, said bacterial active agent comprising a Bacillus genus bacterium and a nitrogen-fixing bacterium, characterized in that the quantity of said Bacillus genus bacterium and said nitrogen-fixing bacterium in said bacterial active agent is greater than or equal to 75% by weight relative to the total weight of said bacterial active agent in the composition. 2025 / 5012 BE2025 / 5012 5 Other embodiments of the use of a composition to increase the growth of a plant according to the present invention are indicated in the annexed claims.The present invention also relates to a biostimulant composition comprising at least one bacterium of the genus Bacillus selected from a group consisting of Bacillus subtilis, Bacillus myloliquefaciens, Bacillus velezensis, Bacillus cereus, Bacillus pumilus, Bacillus megaterium, Bacillus thuringiensis, Bacillus licheniformis, and at least one plant co-product in liquid or solid form, said plant co-product comprising a crude protein content of at least 5%, preferably at least 10% relative to the dry weight of the plant co-product (crude protein weight / dry weight of the plant co-product) and in which said plant co-product in solid form has a median size greater than 250µm.Indeed, a biostimulant composition comprising at least one Bacillus-like bacterium and at least one plant co-product comprising one or more amino acids allows for a significant biostimulant effect on plant growth while being a "simple" composition, easily industrializable, and perfectly integrated into a sustainable development and circular economy process by providing one or more plant co-products as a source of biomolecules. This source of biomolecules stimulates the growth of the Bacillus-like bacterium in the biostimulant composition when this composition is administered to the plant, while also providing useful nutrients directly to the plant to enhance its growth. Other embodiments of the biostimulant composition according to the present invention are indicated in the attached claims. The present invention also relates to a seed of a plant of interest coated with the biostimulant composition according to the present invention.25 Other embodiments of a plant seed according to the present invention are indicated in the attached claims. The present invention also relates to a method for increasing plant growth comprising the step of administering to said plants the biostimulant composition according to the present invention, or the seed of a plant according to the present invention. 30 Other embodiments of the method for increasing plant growth according to the present invention are indicated in the attached claims.2025 / 5012 BE2025 / 5012 6 The present invention also relates to the use of a biostimulant composition for increasing the growth of a plant, said composition comprising at least one bacterium of the genus Bacillus selected from a group consisting of Bacillus subtilis, Bacillus myloliquefaciens, Bacillus velezensis, Bacillus cereus, Bacillus pumilus, Bacillus megaterium, Bacillus thuringiensis, Bacillus licheniformis, and at least one plant co-product in liquid or solid form, said plant co-product comprising a crude protein content of at least 5%, preferably at least 10%, relative to the dry weight of the plant co-product (crude protein weight / dry matter weight). plant co-product) and wherein said plant co-product in solid form has a median size greater than 250µm.10 Other embodiments of the use of a biostimulant composition to increase the growth of a plant according to the present invention are indicated in the attached claims.Detailed description of the invention. Other features, details and advantages of the invention will become apparent from the description given below, by way of non-limiting reference and with reference to examples.