A kind of method extracting paeonol from peony root

Abstract

The invention belongs to the technical field of plant biology and in particular relates to a method for extracting paeonol from paeonia suffruticosa roots. The method comprises the steps of picking, complex enzyme liquid preparation, ultrasonic treatment, centrifugal treatment and reduced pressure distillation. The method has the beneficial effects that enzyme treatment is firstly carried out on paeonia suffruticosa roots and barks at 30 DEG C for 20 minutes, so that the cell walls of the paeonia suffruticosa roots and barks and the interstitial structures of the cell walls can suffer from changes such as local loosening, swelling, collapse and the like; short-time ultrasonic treatment is then carried out on the paeonia suffruticosa roots and barks, ultrasonic waves generate strong impact waves and microjet inside the liquid, and the powerful impact flow can effectively reduce and eliminate the blocking layer between a solvent and an aqueous phase, thus increasing the mass transfer rate; besides, the impact flow generates a physical shear force towards cell tissues, so that the cell tissues deform, fracture and release inclusions, the cell walls and the interstitial structures of the cell walls suffer from local loosening and swelling, the extraction process of paeonol in the cells is accelerated, and the effect of getting twofold results with half the effort is achieved.

Description

technical field

[0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for extracting paeonol from peony roots. Background technique

[0002] Peony is a shrub of Paeoniae genus in Ranunculaceae, and it is also known as Luoyang flower, rich flower and "king of flowers". Its root bark is an important traditional Chinese medicine with important medicinal value, and paeonol is an important active component of the root bark. Paeonol is colorless needle crystal, melting point 49°C-51°C, slightly soluble in water, soluble in hot water, insoluble in cold water, volatile with water vapor, soluble in ethanol, ether, acetone, chloroform, benzene and carbon disulfide. Special smell, slightly spicy storage conditions: cool, dry, away from light. Paeonol has analgesic, anti-inflammatory, antipyretic and anti-allergic effects. It has obvious analgesic effect on pain caused by physical or chemical factors such as tail pressure and acetic a...

Examples

Embodiment Construction

[0017] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0018] A method for extracting paeonol from peony roots, comprising the following steps:

[0019] Step 1. Select the peony that has been cultivated for 4 years without disease, wash the soil, scrape the root bark, put the peony root bark in an oven with a temperature of 50°C and dry it with air blast, then take out the peony root bark and crush it to 40 meshes. spare;

[0020] Step 2. Mix the cellulase aqueous solution with a mass concentration of 0.2 mg / mL and the pectinase aqueous solution with a mass concentration of 0.1 mg / mL in equal volumes, and add an acetic acid solution with a mass percentage concentr...

technical field

[0001] The invention belongs to the field of plant biotechnology, and in particular relates to a method for extracting paeonol from peony roots. Background technique

[0002] Peony is a shrub of Paeoniae genus in Ranunculaceae, and it is also known as Luoyang flower, rich flower and "king of flowers". Its root bark is an important traditional Chinese medicine with important medicinal value, and paeonol is an important active component of the root bark. Paeonol is colorless needle crystal, melting point 49°C-51°C, slightly soluble in water, soluble in hot water, insoluble in cold water, volatile with water vapor, soluble in ethanol, ether, acetone, chloroform, benzene and carbon disulfide. Special smell, slightly spicy storage conditions: cool, dry, away from light. Paeonol has analgesic, anti-inflammatory, antipyretic and anti-allergic effects. It has obvious analgesic effect on pain caused by physical or chemical factors such as tail pressure and acetic a...

Examples

Embodiment Construction

[0017] The embodiments of the present invention are described in detail below. This embodiment is implemented on the premise of the technical solution of the present invention, and detailed implementation methods and specific operating procedures are provided, but the protection scope of the present invention is not limited to the following implementation example.

[0018] A method for extracting paeonol from peony roots, comprising the following steps:

[0019] Step 1. Select the peony that has been cultivated for 4 years without disease, wash the soil, scrape the root bark, put the peony root bark in an oven with a temperature of 50°C and dry it with air blast, then take out the peony root bark and crush it to 40 meshes. spare;

[0020] Step 2. Mix the cellulase aqueous solution with a mass concentration of 0.2 mg / mL and the pectinase aqueous solution with a mass concentration of 0.1 mg / mL in equal volumes, and add an acetic acid solution with a mass percentage concentr...