New preparation method of Tetrastigma hemsleyanum Diels et Gilg extract

Abstract

The invention discloses a technique for extracting flavonoid compounds from Hemsley Rockvine root. The technique flow is as follows: the raw material Hemsley Rockvine root is air-dried, cut up, added with water and put into an ultrasonic extractor together with the water to be extracted; the extract is concentrated into a certain volume; and then active carbon is applied to adsorption bleaching. A cavitation suspension extraction separation device is utilized, acetic ether is first applied to extraction and then recovered; a part of the aqueous solution is extracted by utilizing chloroform which is recovered later; the part of the aqueous solution is concentrated into a solution without organic reagent taste; and through macroporous adsorptive resin, water and 50 percent of ethanol are applied to elution in sequence, 50 percent of ethanol eluant is collected and the solvent is recovered until the mixture is dried, thus obtaining the Hemsley Rockvine root extract.

Description

technical field

[0001] The invention relates to the technical field of medicine, and relates to a high-efficiency extraction process of a trifoliate leaf extract, in particular, the extract containing flavonoids is extracted from the trifoliate greens as a raw material. Background technique

[0002] The methods for extracting proanthocyanidins from Clover are basically traditional chemical solvent extraction and supercritical fluid extraction. However, both extraction processes have many drawbacks. Although the traditional organic solvent extraction method is simple to operate, it has disadvantages such as large energy and raw material consumption, low extraction efficiency, and large industrial pollution emissions. Supercritical CO 2 Although the extraction technology has been greatly improved in extraction efficiency, the equipment required by this type of method is more complicated, the solvent selection range is narrow, and the product stability is poor, which is not c...

Examples

Embodiment Construction

[0010] Further describe the separation and preparation of the Clover extract by the following examples

[0011] Take 100g of Clover, crush it, and extract it in an ultrasonic extractor for 3 times, using 500ml of water each time, combine and concentrate the extracts to a certain volume, then use activated carbon for adsorption and decolorization, and then use a cavitation suspension extraction separation device , first extract with 600ml of ethyl acetate, recover ethyl acetate, extract the aqueous solution with 600ml of chloroform, recover chloroform, concentrate the aqueous solution until there is no organic reagent smell, pass through the macroporous adsorption resin, and then elute with water and 50% ethanol, Collect the 50% ethanol eluate, recover the solvent until dry, and obtain the trifolium clover extract, whose flavonoid content can reach more than 50%.

technical field

[0001] The invention relates to the technical field of medicine, and relates to a high-efficiency extraction process of a trifoliate leaf extract, in particular, the extract containing flavonoids is extracted from the trifoliate greens as a raw material. Background technique

[0002] The methods for extracting proanthocyanidins from Clover are basically traditional chemical solvent extraction and supercritical fluid extraction. However, both extraction processes have many drawbacks. Although the traditional organic solvent extraction method is simple to operate, it has disadvantages such as large energy and raw material consumption, low extraction efficiency, and large industrial pollution emissions. Supercritical CO 2 Although the extraction technology has been greatly improved in extraction efficiency, the equipment required by this type of method is more complicated, the solvent selection range is narrow, and the product stability is poor, which is not c...

Examples

Embodiment Construction

[0010] Further describe the separation and preparation of the Clover extract by the following examples

[0011] Take 100g of Clover, crush it, and extract it in an ultrasonic extractor for 3 times, using 500ml of water each time, combine and concentrate the extracts to a certain volume, then use activated carbon for adsorption and decolorization, and then use a cavitation suspension extraction separation device , first extract with 600ml of ethyl acetate, recover ethyl acetate, extract the aqueous solution with 600ml of chloroform, recover chloroform, concentrate the aqueous solution until there is no organic reagent smell, pass through the macroporous adsorption resin, and then elute with water and 50% ethanol, Collect the 50% ethanol eluate, recover the solvent until dry, and obtain the trifolium clover extract, whose flavonoid content can reach more than 50%.