A centrifuge tube

Abstract

The present invention relates to a centrifuge tube, comprising a centrifuge tube body, a handle, a connector, a seal and a bottom cover, wherein the bottom of the centrifuge tube body has a bottom opening, the bottom cover is connected to the bottom opening, the connector is fixedly connected to the seal and is arranged in the centrifuge tube body, the seal is sealed and connected to the inner wall of the centrifuge tube body, the handle is inserted into the centrifuge tube body and is detachably connected to the connector. The centrifuge tube of the present invention can realize layered liquid extraction and reagent addition, improve extraction efficiency and safety, and save equipment costs.

Description

Technical Field

[0001] The invention relates to the field of medical instruments, in particular to a centrifuge tube. Background Art

[0002] In the field of biological sciences, centrifugation technology has been widely used. Centrifugation technology is one of the most commonly used methods for separating proteins, enzymes, nucleic acids and cell subcomponents, and is also a commonly used separation, purification or clarification method in biochemical laboratories. The sample suspension is placed in a centrifuge tube and rotated at high speed. Due to the huge centrifugal force, the suspended tiny particles (such as organelles, biomacromolecule precipitation, etc.) settle at a certain speed, thereby being separated from the solution.

[0003] The centrifuge tubes currently used are all traditional tube caps and tube bodies. The solution that needs to be centrifuged is added to the centrifuge tube through a pipette. The solution after centrifugation is layered in the centrifuge tube. If you want to obtain the lower layer solution, you need to use a pipette to suck up the upper solution to obtain the lower layer solution. The lower layer solution may be contaminated during the operation, and the operation process is complicated. If you want to obtain solutions of different layers, you need to add liquid and subpackage multiple times. For example, in the process of extracting and freezing adipose stem cells, it is often necessary to extract different layers of tissues after centrifugation, and add liquid and subpackage multiple times. The centrifuge tube needs to be replaced multiple times during the process, which not only causes the large-scale use of centrifuge tubes, but also makes the operation cumbersome and increases the possibility of contamination of solutions of different layers during the operation. Summary of the invention

[0004] The technical problem to be solved by the present invention is to provide a centrifuge tube which can realize layered liquid extraction.

[0005] The technical solution of the present invention for solving the above technical problems is as follows: a centrifuge tube, comprising a centrifuge tube body, a handle, a connecting piece, a sealing piece and a bottom cover, wherein the bottom of the centrifuge tube body has a bottom opening, the bottom cover is connected to the bottom opening, the connecting piece is fixedly connected to the sealing piece and is arranged in the centrifuge tube body, the sealing piece is sealed and connected to the inner wall of the centrifuge tube body, and the handle is inserted into the centrifuge tube body and connected to the connecting piece.

[0006] The beneficial effects of the present invention are as follows: a bottom opening is provided at the bottom of the centrifuge tube body, a sealing member is arranged inside the centrifuge tube body, and a handle is connected to the sealing member through a connecting member. The centrifuge tube of the present invention can discharge or inhale the solution from the bottom opening by pushing and pulling the handle, and the solution does not need to be added with the aid of other instruments such as a pipette, and the lower layer solution after centrifugation can be easily obtained. When the tissue after centrifugation is divided into multiple layers, the different layers of tissue can be pushed out from the bottom opening in sequence, which greatly reduces the steps and time for separating the multi-layer centrifuged tissue. The bottom cover is covered during centrifugation to prevent the solution from overflowing from the bottom opening. The centrifuge tube of the present invention can simultaneously realize layered liquid extraction and reagent addition in one centrifuge tube, and can be packaged for the treated tissue solution, thereby improving the extraction efficiency and safety and saving the equipment cost.

[0007] Based on the above technical solution, the present invention can also be improved as follows.

[0008] Furthermore, the handle includes a pushing body, a pushing rod and a pushing head, the pushing body and the pushing head are connected via the pushing rod, and the pushing head and the connecting member are detachably connected.

[0009] The beneficial effect of adopting the above further solution is that the handle is connected to the connecting piece in the centrifuge tube body through the push head, the push body is connected to the push head through the push rod, the push body is outside the centrifuge tube body, and the handle can be pushed to move by pushing the push body, thereby driving the sealing piece fixedly connected to the connecting piece to move in the tube body. When performing centrifugal operation, the handle can be removed to facilitate the centrifuge tube to be placed in the centrifuge.

[0010] Specifically, the pushing head is fixedly connected to the connecting member; or the pushing head is detachably connected to the connecting member.

[0011] Furthermore, the pushing head includes at least two pushing parts, and at least two of the pushing parts are respectively perpendicular to the pushing rod.

[0012] The beneficial effect of adopting the above further solution is that at least two pushing parts of the pushing head drive the connecting member and the sealing member to move, the force is evenly applied, and the connection is stable.

[0013] Furthermore, the pushing head includes four pushing parts, the four pushing parts are respectively perpendicular to the pushing rod, and two adjacent pushing parts are perpendicular to each other.

[0014] The beneficial effect of adopting the above further solution is that the four pushing parts are in a cross shape, driving the connecting member and the sealing member to move, with uniform force and stable connection.

[0015] Furthermore, the connecting member includes a positioning plate and a connecting ring, the connecting ring is fixedly connected to the sealing member, the positioning plate is fixedly connected to the connecting ring, the positioning plate has a through hole adapted to the pushing head, and the inner ring of the connecting ring has at least one limiting portion for limiting the rotation angle of the pushing portion.

[0016] The beneficial effect of adopting the above further scheme is that the push head is inserted into the through hole of the positioning plate, inserted into the connecting ring, and the push head is rotated to abut against the limit part, and the push head cannot be pulled out from the through hole of the positioning plate. The push head can drive the seal to reciprocate in the centrifuge tube body by cooperating with the positioning plate and the connecting ring. The push head is detachably connected to the positioning plate and the connecting ring. The handle is installed when the solution in the centrifuge tube body needs to be inhaled or discharged, and the handle is removed when it is placed in a centrifuge for centrifugation. While the centrifuge tube of the present invention realizes the functions of layered liquid extraction and reagent addition, the handle can be removed for easy use in a centrifuge.

[0017] Specifically, the thickness of the connecting ring is greater than the thickness of the pushing head.

[0018] Furthermore, the connecting member is a locking plate, the locking plate has a locking through hole adapted to the pushing head, the locking plate is fixedly connected to the sealing member, and the side of the locking plate connected to the sealing member has a fan-shaped locking groove opening downward.

[0019] The beneficial effect of adopting the above further scheme is that the push head is inserted into the locking through hole of the locking plate, and after the push head contacts the sealing member, the push head is rotated, and after the push head rotates a certain angle in the fan-shaped locking groove, the push head is locked with the locking plate. The push head can drive the sealing member to reciprocate in the centrifuge tube body. The push head and the locking plate are detachably connected, and the handle is installed when the solution in the centrifuge tube body needs to be inhaled or discharged, and the handle is removed when the centrifuge is placed in a centrifuge for centrifugation. While the centrifuge tube of the present invention realizes the functions of layered liquid extraction and reagent addition, the handle can be removed for easy use in a centrifuge.

[0020] Specifically, the depth of the locking groove is greater than the thickness of the pushing head.

[0021] Furthermore, it also includes a top cover, which is connected to the top opening of the centrifuge tube body, and the top cover has a handle hole for the handle to pass through.

[0022] The beneficial effect of adopting the above further solution is that the top cover can allow the handle to pass through, and the centrifuge tube body is prevented from being contaminated by bacteria, thereby ensuring a sterile environment inside the centrifuge tube body.

[0023] Specifically, the top cover is interference fit with the centrifuge tube body; or the top cover is snap-fitted with the centrifuge tube body.

[0024] Furthermore, the inner wall of the centrifuge tube body has a clamping portion, and the side surface of the top cover is provided with a clamping groove corresponding to the clamping portion, and the top cover is clamped with the centrifuge tube body.

[0025] The beneficial effect of adopting the above further solution is that the top cover is snap-fitted with the centrifuge tube body, the connection is stable, and at the same time, the connector, the seal and the top cover can be prevented from being separated from the centrifuge tube body during centrifugation.

[0026] Furthermore, it also includes a top cap, which is detachably sealed and connected to the top cover.

[0027] The beneficial effect of adopting the above further solution is that the handle is removed before centrifugation, and the top cap is covered on the top cover, so that the centrifuge tube body has good sealing performance.

[0028] Specifically, the top cap has a protrusion with the same shape and size as the handle hole of the top cover, and the protrusion is matched and connected with the handle hole.

[0029] Furthermore, a scale is provided on the side wall of the centrifuge tube body.

[0030] The beneficial effect of adopting the above further scheme is that a fixed amount of solution can be sucked in or discharged using a centrifuge tube according to a scale. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a structural schematic diagram of a centrifuge tube of the present invention;

[0032] Figure 2 A cross-sectional view of a centrifuge tube of the present invention;

[0033] Figure 3 This is a schematic diagram of the handle structure of a centrifuge tube of the present invention;

[0034] Figure 4 It is a structural schematic diagram of a centrifuge tube positioning plate of the present invention;

[0035] Figure 5 This is a structural schematic diagram of a centrifuge tube connecting ring of the present invention;

[0036] Figure 6 This is a structural schematic diagram of a centrifuge tube locking plate of the present invention;

[0037] Figure 7 It is a structural schematic diagram of a centrifuge tube seal of the present invention;

[0038] Figure 8 This is a structural schematic diagram of a centrifuge tube top cover of the present invention;

[0039] Fig. 9 It is a structural schematic diagram of a centrifuge tube body of a centrifuge tube of the present invention;

[0040] Fig.10 The present invention is a schematic structural diagram of a centrifuge tube top cap.

[0041] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0042] 1. Handle, 101. Pushing body, 102. Pushing rod, 103. Pushing head, 1031. Pushing part, 2. Top cover, 3. Centrifuge tube body, 4. Positioning plate, 5. Connecting ring, 501. Limiting part, 6. Sealing element, 7. Bottom cover, 8. Top cap, 9. Locking plate. DETAILED DESCRIPTION

[0043] The principles and features of the present invention are described below. The examples given are only used to explain the present invention and are not used to limit the scope of the present invention.

[0044] Example 1

[0045] like Figure 1 and Figure 2 As shown, a centrifuge tube includes a centrifuge tube body 3, a handle 1, a connecting piece, a sealing piece 6 and a bottom cover 7. The bottom of the centrifuge tube body 3 has a bottom opening, the bottom cover 7 is connected to the bottom opening, the connecting piece is fixedly connected to the sealing piece 6 and is arranged in the centrifuge tube body 3, the sealing piece 6 is sealed and connected to the inner wall of the centrifuge tube body 3, and the handle 1 is inserted into the centrifuge tube body 3 and connected to the connecting piece.

[0046] Specifically, the centrifuge tube body 3 is a hollow tube body, the side wall of the centrifuge tube body 3 is cylindrical, and the bottom is funnel-shaped.

[0047] Specifically, the connecting member is bonded to the sealing member 6 .

[0048] Specifically, the sealing member 6 is made of rubber.

[0049] Preferably, the centrifuge tube body 3 is made of medical plastic.

[0050] Specifically, the bottom cover 7 has a threaded hole in the middle, the bottom opening has an external thread on the outside, and the bottom cover 7 is threadedly connected to the bottom opening. The outer diameter of the bottom cover 7 is the same as the outer diameter of the centrifuge tube body, and the outer surface of the bottom cover 7 has anti-slip patterns. The bottom opening has a diameter of 0.5 cm.

[0051] As a further solution of this embodiment, Figure 3 As shown, the handle 1 includes a pushing body 101, a pushing rod 102 and a pushing head 103. The pushing body 101 is connected to the pushing head 103 via the pushing rod 102, and the pushing head 103 is detachably connected to the connecting member.

[0052] As a further solution of this embodiment, the pushing head 103 includes at least two pushing parts 1031 , and the at least two pushing parts 1031 are respectively perpendicular to the pushing rod 102 .

[0053] As a further solution of this embodiment, the pushing head 103 includes four pushing parts 1031 , the four pushing parts 1031 are respectively perpendicular to the pushing rod 102 , and two adjacent pushing parts 1031 are perpendicular to each other.

[0054] Specifically, the four pushing parts 1031 of the pushing head are perpendicular to the pushing rod 102 and are in a cross shape and on the same plane.

[0055] As a further solution of this embodiment, Figure 4 and Figure 5 As shown, the connecting member includes a positioning plate 4 and a connecting ring 5, the connecting ring 5 is fixedly connected to the sealing member 6, the positioning plate 4 is fixedly connected to the connecting ring 5, the positioning plate 4 has a through hole adapted to the pushing head 103, and the inner ring of the connecting ring 5 has at least one limiting portion 501 for limiting the rotation angle of the pushing portion 1031.

[0056] Specifically, Figure 4 and Figure 5 As shown, the positioning plate 4 has a cross-shaped through hole with the same cross-sectional shape and size as the push head 103. The thickness of the connecting ring 5 is greater than the thickness of the push head 103. The push head 103 can rotate in the connecting ring 5. Figure 5 As shown, the inner ring of the connecting ring 5 has four limiting parts 501, and the limiting parts 501 are protrusions extending from the inner ring of the connecting ring 5 toward the center of the circle, and two adjacent limiting parts 501 are perpendicular to each other. The connecting ring 5 is bonded to the sealing member 6, and the positioning plate 4 is bonded to the connecting ring 5. The limiting parts 501 of the connecting ring 5 are staggered with the cross-shaped through holes of the positioning plate 4, and the angle between the center line of each limiting part 501 and the center line of the through hole is 45 degrees. The pushing head 103 is inserted into the through hole of the positioning plate, and then inserted into the connecting ring 5. The rotating pushing body 101 drives the pushing head 103 to rotate in the connecting ring 5 until the pushing part 1031 of the pushing head 103 abuts against the limiting part 501, the pushing part 1031 is staggered with the cross through hole of the positioning plate 4, and the pushing part 1031 is restricted by the positioning plate 4 and cannot be disengaged, and the handle 1 is locked with the positioning plate 4 and the connecting ring 5. The sealing member 6 can be driven to move in the centrifuge tube body 3 by pushing and pulling the handle 1.

[0057] As a further solution of this embodiment, Figure 8 As shown, a top cover 2 is also included, and the top cover 2 is connected to the top opening of the centrifuge tube body 3, and the top cover 2 has a handle hole for the handle 1 to pass through.

[0058] Specifically, the top cover 2 is connected with the centrifuge tube body 3 by interference fit. The top cover 2 has a cross-shaped handle hole with the same cross-sectional shape and size as the push head 103.

[0059] Alternative, such as Figure 8 As shown, the inner wall of the centrifuge tube body 3 has a clamping portion, and the side surface of the top cover 2 is provided with a clamping groove corresponding to the clamping portion, and the top cover is clamped with the centrifuge tube body 3.

[0060] Specifically, Fig. 9 As shown, the diameter of the top opening of the centrifuge tube body 3 is larger than the diameter of the lower part of the centrifuge tube body, so as to avoid the clamping part hindering the disassembly of the connector and the seal in the centrifuge tube body, and facilitate the disassembly, cleaning and reuse of the centrifuge tube.

[0061] As a further solution of this embodiment, Fig.10 As shown, a top cap 8 is also included, and the top cap 8 is detachably sealedly connected to the top cover 2.

[0062] Specifically, the handle 1 is removed before centrifugation, and the top cap 8 is covered on the top cover 2 to ensure the sealing of the centrifuge tube body 3. The top cap 8 has a raised portion with the same shape and size as the handle hole of the top cover 2, and the raised portion is matched and connected with the handle hole.

[0063] As a further solution of this embodiment, a scale is provided on the side wall of the centrifuge tube body 3 .

[0064] The volume of the centrifuge tube in this embodiment can be designed according to experimental requirements.

[0065] The specific use process of this embodiment is as follows:

[0066] Inhaling solution: Remove the top cap 8 and the bottom cover 7, and insert the push head 103 of the handle 1 through the handle hole of the top cover 2, through the cross hole of the positioning plate 4, and then insert it into the connecting ring 5. At this time, the pusher 101 is located outside the centrifuge tube body 3. Rotating the pusher 101 drives the pusher head 103 to rotate in the connecting ring 5 until the push portion 1031 of the pusher head 103 abuts against the limiting portion 501, and the push portion 1031 is staggered from the cross hole of the positioning plate 4. Extend the bottom opening into the liquid to be centrifuged, pull the handle 1, and the push portion 1031 is restricted by the positioning plate 4 and cannot come out, thereby driving the seal 6 to move away from the bottom opening to inhale the liquid. According to the scale, a quantitative liquid can be accurately inhaled.

[0067] Centrifugation: Cover the bottom cover 7. Turn the handle 1 so that the push part 1031 is aligned with the cross hole of the positioning plate 4, and pull the handle 1 out of the positioning plate 4 and the top cover 2. Cover the top cap 8. Put the centrifuge tube into the centrifuge and perform centrifugation.

[0068] The centrifuge tube of the present invention can be used in situations where multiple layers of tissue need to be extracted after centrifugation. For example, in the process of extracting adipose stem cells, it is often necessary to extract different layers of tissue after centrifugation; centrifuging blood to obtain red blood cells, white blood cells and serum, etc.

[0069] Drain the solution: remove the top cap 8 and the bottom cover 7, pass the push head 103 of the handle 1 through the handle hole of the top cover 2, through the cross hole of the positioning plate 4, and then insert it into the connecting ring 5. At this time, the pusher 101 is located outside the centrifuge tube body 3. Rotating the pusher 101 drives the pusher head 103 to rotate in the connecting ring 5 until the push portion 1031 of the pusher head 103 abuts against the limiting portion 501, and the push portion 1031 is offset from the cross hole of the positioning plate 4. Push the handle 1, and then drive the seal 6 to move toward the bottom, discharge and collect the liquid at the bottom layer. In order to avoid contamination of solutions at different levels, the liquid near the solution layer is discharged and discarded, and then the liquid of the previous layer continues to be collected.

[0070] When the centrifugal speed is high, some large particles in the tissue homogenate will precipitate at the bottom of the centrifuge tube. These precipitates are semi-solid substances. The seal 6 moves toward the bottom of the centrifuge tube body 3, and the pressure in the tube body increases. The centrifuge tube of the present invention can easily discharge the lower precipitate in the tube body.

[0071] The centrifuge tube of the present invention is a disposable centrifuge tube, and can also be a centrifuge tube that is repeatedly used for multiple centrifugation.

[0072] Example 2

[0073] On the basis of Example 1, Figure 6 As shown, the connecting member can also be a locking plate 9, which has a locking through hole adapted to the pushing head 103, and the locking plate 9 is fixedly connected to the sealing member 6. The side where the locking plate 9 is connected to the sealing member 6 has a fan-shaped locking groove opening downward.

[0074] Figure 6 The locking plate 9 shown in the figure is a view of the locking plate 9 facing the sealing member 6, and the locking plate 9 is bonded to the sealing member 6. The locking plate 9 has a cross through hole, and the push head 103 of the handle 1 passes through the handle hole of the top cover 2 and the cross through hole of the locking plate 9 until it contacts the sealing member 6. At this time, the pusher 101 is located outside the centrifugal tube body 3, and the rotating pusher 101 drives the pusher 103 to rotate in the fan-shaped locking groove of the locking plate 9 until the push portion 1031 of the pusher 103 abuts against the side wall of the locking groove, the push portion 1031 is staggered with the cross through hole of the locking plate 9, and the push portion 1031 is blocked by the top surface of the fan-shaped locking groove and cannot be pulled out from the cross through hole of the locking plate 9. The pusher 103 is locked with the locking plate 9, and the push-pull handle 1 drives the locking plate 9 and the sealing member 6 to move in the centrifugal tube body 3 to achieve layered liquid extraction and reagent addition.

[0075] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A centrifuge tube, characterized in that: The centrifuge tube (3) comprises a centrifuge tube body (3), a handle (1), a connecting piece, a sealing piece (6) and a bottom cover (7), wherein the bottom of the centrifuge tube body (3) has a bottom opening, the bottom cover (7) is connected to the bottom opening, the connecting piece is fixedly connected to the sealing piece (6) and is arranged in the centrifuge tube body (3), the sealing piece (6) is sealed and connected to the inner wall of the centrifuge tube body (3), the handle (1) is inserted into the centrifuge tube body (3) and connected to the connecting piece; the centrifuge tube body (3) comprises a top cover (2), the top cover (2) is connected to the top opening of the centrifuge tube body (3), the top cover (2) has a handle hole for the handle (1) to pass through; the centrifuge tube body (3) comprises a top cap (8), the top cap (8) is detachably sealed and connected to the top cap (2); the top cap (8) has a protrusion having the same shape and size as the handle hole of the top cover (2), the protrusion is matched and connected to the handle hole; The handle (1) comprises a pushing body (101), a pushing rod (102) and a pushing head (103); the pushing body (101) and the pushing head (103) are connected via the pushing rod (102); and the pushing head (103) and the connecting member are detachably connected; The pushing head (103) comprises at least two pushing parts (1031), and the at least two pushing parts (1031) are respectively perpendicular to the pushing rod (102); The connecting member comprises a positioning plate (4) and a connecting ring (5), the connecting ring (5) is fixedly connected to the sealing member (6), the positioning plate (4) is fixedly connected to the connecting ring (5), the positioning plate (4) has a through hole adapted to the pushing head (103), and the inner ring of the connecting ring (5) has at least one limiting portion (501) for limiting the rotation angle of the pushing portion (1031); Alternatively, the connecting member is a locking plate (9), the locking plate (9) having a locking through hole adapted to the pushing head (103), the locking plate (9) being fixedly connected to the sealing member (6), and the side where the locking plate (9) is connected to the sealing member (6) having a fan-shaped locking groove opening downward.

2. A centrifuge tube according to claim 1, characterized in that: The pushing head (103) comprises four pushing parts (1031), the four pushing parts (1031) are respectively perpendicular to the pushing rod (102), and two adjacent pushing parts (1031) are perpendicular to each other.

3. A centrifuge tube according to claim 1, characterized in that: The inner wall of the centrifuge tube body (3) has a clamping portion, and the side surface of the top cover (2) is provided with a clamping groove corresponding to the clamping portion, and the top cover is clamped with the centrifuge tube body (3).

4. A centrifuge tube according to any one of claims 1 to 3, characterized in that: The side wall of the centrifuge tube body (3) is provided with a scale.

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