Fully automatic microbial identification and drug sensitivity analysis system

Abstract

The present invention discloses a fully automatic microbial identification and drug sensitivity analysis system, including a whole machine housing, in which a sample loading unit, a sample identification and recording unit, a plate loading unit, an incubation unit, a detection unit and a board transfer unit are arranged in the inner cavity of the whole machine housing; the sample loading unit automatically completes loading and unloading of the gun head, liquid aspiration of the gun body and sample loading of the board; the sample identification and recording unit is located on one side of the sample loading unit, and scans and records the sample bottle containing the sample and automatically selects it; the plate loading unit is located on the other side of the sample loading unit, and is used to load and scan and record the board; the incubation unit incubates the board after sample loading; the detection unit identifies and performs drug sensitivity detection on the incubated board; the board transfer unit serves as a connection mechanism between the plate loading unit, the sample loading unit and the incubation unit, and transfers the board from the plate loading unit to the sample loading unit, and then the sample loading unit transfers and pushes the board into the incubation unit. The advantages of the present invention are simple and convenient operation, large sample processing capacity and high degree of automation.

Description

Technical Field

[0001] The present invention relates to the field of microbial detection, and in particular to a fully automatic microbial identification and drug sensitivity analysis system. Background Art

[0002] Antibacterial drugs are the most commonly used drugs in clinical practice. They are effective in treating bacterial infections and bacterial infections and are widely used by doctors. However, in recent years, the abuse of antibiotics has become very common, which not only reduces the efficacy, but also has large toxic and side effects of drug sensitivity, increases bacterial resistance, loses the opportunity for treatment and rescue, and harms the health and life of patients. Since the temperature and humidity required for the cultivation of various bacteria and microorganisms are different, it is often necessary to artificially create the required environment when conducting molecular biology and bacterial culture. Once there are many types of bacteria and microorganisms that need to be cultivated, it is easy to increase the workload of medical staff and may cause operational errors, which brings great inconvenience to medical staff.

[0003] With the continuous development of science and technology, people have higher and higher requirements for the automation of medical testing equipment. However, the existing microbial identification and drug sensitivity analyzers usually only have incubation and analysis functions, with low automation. They need to manually add samples and transfer boards outside the analyzer, and then put them into the instrument for incubation and analysis. In addition, the amount of specimens processed at one time is small, the manual operation content is large, and the unforeseen risks are large, which increases the workload of operators. This instrument is far from meeting the growing demand for microbial testing. At present, the market is in urgent need of a microbial identification and drug sensitivity analysis equipment with high automation, simple operation, and large specimen processing capacity. Summary of the invention

[0004] The present invention aims to provide a fully automatic microbial identification and drug sensitivity analysis system which is simple to operate and has a large sample processing capacity.

[0005] To achieve the above purpose, the present invention can adopt the following technical solutions:

[0006] The fully automatic microbial identification and drug sensitivity analysis system of the present invention comprises a complete machine housing, in the inner cavity of which are arranged a sample adding unit, a sample identification and recording unit, a plate carrying unit, an incubation unit, a detection unit and a plate card transfer unit;

[0007] The sample adding unit automatically completes the loading and unloading of the gun head, the liquid aspiration of the gun body and the board adding of the sample;

[0008] The sample identification and recording unit is located on one side of the sample adding unit, and scans, records and automatically selects the sample bottles containing the samples;

[0009] The plate loading unit is located at the other side of the sample loading unit and is used for loading and scanning recording plates and cards;

[0010] The incubation unit is used to incubate the sample-added board;

[0011] The detection unit performs identification and drug sensitivity test on the incubated board and card;

[0012] The board transfer unit serves as a connection mechanism between the board carrier unit, the sample loading unit and the incubation unit, and transfers the board from the board carrier unit to the sample loading unit, and then transfers and pushes the board from the sample loading unit to the incubation unit.

[0013] The loading unit includes a support frame installed in the whole machine shell, and a back plate fixedly arranged longitudinally between two columns on the rear side of the support frame; an X-axis synchronous belt transmission mechanism driven by an X-axis motor is horizontally arranged on the rear side of the back plate, a cantilever arm extending horizontally forward is fixedly connected to the synchronous belt of the X-axis synchronous belt transmission mechanism, a Y-axis synchronous belt transmission mechanism driven by a Y-axis motor is horizontally arranged on the cantilever arm, a loading arm extending vertically downward is fixedly connected to the synchronous belt of the Y-axis synchronous belt transmission mechanism, a Z-axis synchronous belt transmission mechanism driven by a Z-axis motor is longitudinally arranged on the loading arm, a loading gun with a liquid suction port vertically downward is fixedly connected to the synchronous belt of the Z-axis synchronous belt transmission mechanism; a microplate supporting device and a gun head loading and unloading device are arranged below the movement track of the loading arm.

[0014] The sample adding gun comprises a liquid suction pump motor fixed on the synchronous belt of the Z-axis synchronous belt transmission mechanism, the working shaft of the liquid suction pump motor is vertically downward, and a synchronously rotating transmission screw is fixedly connected to its lower end through a coupling, and a screw nut that moves up and down along its length direction is provided on the transmission screw, and a piston-type liquid suction pump gun fixedly connected to the housing of the liquid suction pump motor is vertically downwardly provided below the transmission screw, the upper end piston rod of the piston-type liquid suction pump gun is fixedly connected to the screw nut, and a disposable liquid suction gun head located in the gun head storage box is movably mounted on the liquid suction port at its lower end;

[0015] The microplate supporting device comprises a supporting base, on which a rotating spindle driven by a substrate driving motor is vertically penetrated, and the upper end of the rotating spindle is fixedly connected to the center of the bottom surface of a horizontally arranged supporting substrate; two sets of microplate supporting assemblies are fixedly arranged side by side on the supporting substrate, and each set of the microplate supporting assemblies comprises a set of microplate brackets and a set of plate cover clamping frames, the height of the plate cover clamping frames is higher than the height of the microplate brackets, and the microplate brackets and plate cover clamping frames on the two sets of microplate supporting assemblies are arranged alternately;

[0016] The gun tip loading and unloading device includes a gun tip discard bin arranged on the front side of the microplate supporting device, a discarded gun tip storage bucket for extracting or putting in from the front side of the whole machine shell is arranged in the gun tip discard bin, a gun tip dropping opening is opened on the top wall of the gun tip discard bin, a support table with a gun tip containing box on the upper surface is arranged above the gun tip dropping opening, and a gourd-shaped gun tip removal opening corresponding to the gun tip dropping opening is opened on the support table.

[0017] The sample identification and recording unit comprises a sample support seat arranged below the movement track of the sample adding arm, a sample tray rotating shaft driven by the identification and recording unit motor is vertically penetrated on the sample support seat, a sample bottle tray connecting plate is horizontally fixedly connected to the upper end of the sample tray rotating shaft, an annular tray is movably placed on the sample bottle tray connecting plate, a plurality of card slots for inserting sample bottles are evenly distributed along the circumference of the annular tray, and a product factory barcode and a patient information barcode are respectively pasted on the inner and outer side walls of the sample bottle; barcode scanners corresponding to the positions of the product factory barcode and the patient information barcode on the same sample bottle are respectively fixed on the sample support seats inside and outside the annular tray; an annular code disk is fixedly connected to the lower surface of the sample bottle tray connecting plate, and a code disk origin position sensor and a code disk graduation position sensor are arranged on the sample support seat located on one side of the annular code disk.

[0018] The plate carrier unit comprises a fixed base arranged on one side of the microplate supporting device, and a plate release mechanism is fixedly connected to the fixed base through a pair of longitudinal support plates arranged at intervals;

[0019] The board release mechanism comprises a bottom cover fixedly mounted horizontally on the two longitudinal support plates, a board release opening is formed on the bottom cover, and an opening and closing plate with a rectangular ring structure is movably mounted on the upper edge of the board release opening through a guide pin fixed on the bottom cover; the opening and closing plate is formed by a pair of U-shaped thin plates that move towards each other, and the butt joints of the two U-shaped thin plates are connected as a whole through a rotating sleeve, and a group of mutually symmetrical claws are arranged on opposite sides of the bottom wall thin plates of the two U-shaped thin plates, wherein a return spring with an outer end connected to the bottom cover is arranged on one of the U-shaped thin plates, and the stretching direction of the return spring is consistent with the opening and closing direction of the U-shaped thin plate; a A board and card release motor, a board and card release screw is coaxially fixedly connected to the working shaft of the board and card release motor, the axial direction of the board and card release screw is consistent with the opening and closing direction of the two U-shaped thin plates, a nut seat for driving the two U-shaped thin plates to open and close is arranged on the board and card release screw, and a pair of stroke position sensors for monitoring the movement stroke of the nut seat are arranged at intervals on the board and card release motor; a connecting base fixedly connected to the bottom cover is buckled above the two U-shaped thin plates, a board and card drop opening consistent with the board and card release opening is opened on the connecting base, and a U-shaped through groove corresponding to the position of the information barcode on the microplate is opened on one side of the board and card drop opening;

[0020] A board and card stacking mechanism is arranged on the card release mechanism, and the board and card stacking mechanism includes blocking columns vertically fixed at the four corners of the board and card drop opening, and the upper ends of the blocking columns are connected as a whole by connecting the top plate to form a board and card stacking bin adapted to the microplate, and a magnetic suction door with a handle is hinged on one side of the board and card stacking bin, and a Hall sensor is installed on the blocking column attracted by the magnetic suction door.

[0021] The incubation unit comprises an incubation box arranged at one side of the sample loading unit, an incubation base is arranged in the incubation box, a thin-walled bearing is horizontally arranged on the upper surface of the incubation base, the inner ring of the thin-walled bearing is fixedly connected to the incubation base, and a driven pulley driven by the incubation motor is fixedly sleeved on the outer ring thereof, the upper surface of the driven pulley is fixedly connected to a coaxially arranged horizontal incubation tray, a plurality of groups of incubation towers with openings facing outward are uniformly distributed along the circumference on the horizontal incubation tray, a plurality of layers of microplate slots are longitudinally spaced in the incubation towers, and each group of incubation towers is fixed to the upper surface of the horizontal incubation tray by pins;

[0022] A board inlet and a board outlet are provided on the side wall of the incubator, the board inlet is composed of a plurality of board inlet door holes longitudinally spaced apart, the board inlet door holes correspond one-to-one to the microplate slots, and a push door is hinged on the board inlet door hole through an automatic rebound hinge; the board outlet is sealed and connected to the front side wall of the whole machine shell, the size of the board outlet is adapted to the size of the outer opening of a group of incubation towers, and an incubator compartment door is hinged on the front side wall of the whole machine shell corresponding to the board outlet; a heating fan with a heating wire is provided on the top wall of the incubator box, a temperature sensor is provided in the incubator, and a temperature information display screen is provided on the front side wall of the whole machine shell below the incubator compartment door.

[0023] A transmission is arranged on the incubation base, and the transmission is fixedly connected to the lower surface of the incubation base through a fixing plate, and the output end of the transmission vertically extends upward to the top of the incubation base, and a driving pulley is arranged thereon, and the driving pulley is connected to the driven pulley through a transmission belt, and the input end of the transmission is connected to the incubation motor through a synchronous transmission mechanism; a plurality of incubation information indicator lights are arranged on the edge of the plate outlet corresponding to the opening side of the incubation box door, and the incubation information indicator lights correspond one-to-one to a group of microplate slots on the incubation tower.

[0024] The detection unit comprises a detection base arranged at the inner center of the incubation tower, a first guide rail is longitudinally arranged on the detection base, an information collection mechanism driven to rise and fall by a first power mechanism is slidably arranged on the first guide rail, and a counterweight mechanism is arranged on the detection base to prevent the information collection mechanism from falling;

[0025] The information collection mechanism includes a horizontal lifting frame slidably arranged on the first guide rail, a second guide rail is horizontally arranged in the horizontal lifting frame close to the long side wall, a slider driven by a second power mechanism is slidably arranged on the second guide rail, a tray connecting block is horizontally fixedly connected to one side of the slider, a detection tray is fixedly connected to the side of the tray connecting block away from the slider, and the detection tray passes through the tray inlet and outlet opened on the short side wall of the horizontal lifting frame; an optical fiber fixing block is arranged at the lower edge of the tray inlet and outlet, an LED lamp is arranged at the bottom of the horizontal lifting frame, and the light emitted by the LED lamp is irradiated on the detection tray by the optical fiber through the optical fiber fixing block, and the top of the horizontal lifting frame is covered with a photoelectric signal conversion module.

[0026] The counterweight mechanism includes a gate-shaped frame vertically arranged on the detection base, the first guide rail and the information acquisition mechanism are located on the inner side of the gate-shaped frame, a third guide rail is longitudinally arranged on the detection base behind the gate-shaped frame, a hanging shaft located above the photoelectric signal conversion module is installed on the horizontal lifting frame, the hanging shaft is horizontally arranged along the width direction of the gate-shaped frame, and a steel cable with one end fixed on the hanging shaft passes around the fixed pulley on the top of the gate-shaped frame and is fixedly connected to the counterweight block slidably arranged on the third guide rail.

[0027] The board transfer unit includes a longitudinal base plate fixedly attached to the front side of the back plate, a transverse transmission belt driven by a transverse driving motor is horizontally arranged on the front side of the longitudinal base plate, a plurality of arm plate position sensors are arranged at intervals along the length direction of the longitudinal base plate located below the transverse transmission belt, the conveying end of the transverse transmission belt is adapted to correspond to the board inlet of the incubator, and a longitudinal arm plate is fixed on the transverse transmission belt;

[0028] A longitudinal drive motor is arranged at one end of the longitudinal arm plate, and the end of the working shaft of the longitudinal drive motor is connected to a synchronously rotating lead screw located at the front side of the longitudinal arm plate through a coupling, the axial direction of the synchronously rotating lead screw is consistent with the length direction of the longitudinal arm plate, and a lead screw nut that slides up and down is arranged on the synchronously rotating lead screw, and a height positioning plate that is consistent with the length direction of the synchronously rotating lead screw is arranged on the longitudinal arm plate, and a microplate transfer support bracket and a nut position sensor that is compatible with the height positioning plate are arranged on the lead screw nut;

[0029] The microplate transfer support bracket includes a board transfer cantilever arm fixedly connected to the screw nut and extending horizontally forward, a board transfer bracket matching the board entry door opening is horizontally fixedly connected to the board transfer cantilever arm, and a bracket in-place sensor is arranged on the side of the board transfer bracket.

[0030] The advantages of the present invention are simple and convenient operation, large sample processing capacity and high degree of automation. Through the sample loading unit, sample identification and recording unit, plate loading unit, incubation unit, detection unit and board transfer unit arranged in the inner cavity of the whole machine shell, a series of actions including scanning and recording of samples before loading, automatic selection, automatic installation of disposable liquid pipetting gun head before liquid aspiration by the sample loading gun, automatic board loading of the board, liquid aspiration and sample loading by the sample loading gun, removal of disposable liquid pipetting gun head after loading, automatic transfer of the board after loading, board incubation and identification and drug sensitivity test of the board after incubation are completed are completed; the one-time batch is large, which meets the growing detection needs and meets the development needs of the drug sensitivity industry, realizes fully closed automatic operation of loading, transfer, incubation and interpretation, improves work efficiency, reduces manual intervention, truly realizes the goal of 24-hour unmanned operation, and effectively avoids the risks caused by manual intervention; in addition, the overall modular design is adopted, and each module can be disassembled separately, which is very convenient for later maintenance and maintenance. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 It is a structural schematic diagram of the present invention.

[0032] Figure 2 yes Figure 1 Top view (the entire machine casing is hidden).

[0033] Figure 3 , Figure 4 yes Figure 1 The schematic diagram of the internal structure of the whole machine casing and the incubator is hidden.

[0034] Figure 5 , Figure 6 yes Figure 1 Schematic diagram of the structure of the sample addition unit.

[0035] Figure 7 yes Figure 1 Magnified view of the pipetting gun in the pipetting unit.

[0036] Figure 8 yes Figure 1 Schematic diagram of the structure of the microporous plate supporting device.

[0037] Fig. 9 yes Figure 8 Stereoscopic diagram.

[0038] Fig.10 yes Figure 1 Schematic diagram of the structure of the sample recognition recording unit.

[0039] Fig.11 yes Fig.10 Stereoscopic diagram.

[0040] Fig.12 yes Fig.10Magnified image of the sample bottle.

[0041] Fig.13 yes Figure 1 Schematic diagram of the exploded structure of the carrier board unit.

[0042] Fig.14 yes Fig.13 Magnified view of the mid-plate release mechanism.

[0043] Fig.15 yes Fig.13 Magnified view of the microplate.

[0044] Fig.16 yes Figure 1 Schematic diagram of the structure of the incubation unit.

[0045] Fig.17 , Fig.18 yes Fig.16 The structural schematic diagram of the incubator is omitted.

[0046] Fig.19 yes Figure 1 Schematic diagram of the structure of the detection unit.

[0047] Fig. 20 yes Fig.19 Stereoscopic diagram.

[0048] Fig.21 yes Fig.19 Schematic diagram of the structure of the information collection mechanism (the second power mechanism is hidden).

[0049] Fig. 22 , Fig.23 yes Figure 1 Schematic diagram of the structure of the mid-board transfer unit.

[0050] Fig.24 yes Fig. 22 Magnified view of the microplate transfer support (microplate hidden). DETAILED DESCRIPTION

[0051] like Figure 1-4 As shown, the fully automatic microbial identification and drug sensitivity analysis system of the present invention includes an entire housing 1, in which a sample adding unit 2, a sample identification and recording unit 3, a plate carrier unit 4, an incubation unit 5, a detection unit 6 and a board transfer unit 7 are arranged in the inner cavity of the entire housing 1; the sample adding unit 2, the sample identification and recording unit 3, the plate carrier unit 4, the incubation unit 5, the detection unit 6 and the board transfer unit 7 are all controlled by a PLC controller.

[0052] The sample adding unit 2 can automatically complete the loading and unloading of the gun head, the liquid aspiration of the gun body and the board loading; Figure 5 , 6As shown, the loading unit 2 includes a support frame 201 installed in the whole machine housing 1, and the support frame 201 is composed of four longitudinal columns fixed on the inner bottom wall of the whole machine housing 1 and a connecting beam fixedly connected to the upper end of the longitudinal columns. A back plate 202 is fixedly arranged longitudinally between the two columns on the rear side of the support frame 201; an X-axis synchronous belt transmission mechanism 204 driven by an X-axis motor 203 is horizontally arranged on the rear side of the back plate 202, and a suspension belt that passes over the top of the back plate 202 and extends horizontally forward is fixedly connected to the synchronous belt of the X-axis synchronous belt transmission mechanism 204. An arm 205 is provided with a Y-axis synchronous belt transmission mechanism 207 driven by a Y-axis motor 206 horizontally, a loading arm 208 extending vertically downward is fixedly connected to the synchronous belt of the Y-axis synchronous belt transmission mechanism 207, a Z-axis synchronous belt transmission mechanism 210 driven by a Z-axis motor 209 is longitudinally provided on the loading arm 208, a loading gun with a liquid aspiration port vertically downward is fixedly connected to the synchronous belt of the Z-axis synchronous belt transmission mechanism 210, and a microplate supporting device and a gun head loading and unloading device are provided below the movement track of the loading arm 208.

[0053] The cantilever arm 205 moves back and forth along the X-axis direction of the support frame 201 driven by the X-axis synchronous belt transmission mechanism 204, the loading arm 208 moves back and forth along the Y-axis direction of the support frame 201 driven by the Y-axis synchronous belt transmission mechanism 207, and the loading gun moves back and forth along the Z-axis direction of the support frame 201 driven by the Z-axis synchronous belt transmission mechanism 210, thereby realizing the movement of the loading gun in any direction.

[0054] To ensure that the cantilever arm 205 moves stably with the X-axis synchronous belt transmission mechanism 204, an X-axis guide rail 211 consistent with the length direction of the X-axis synchronous belt transmission mechanism 204 is provided on the back plate 202, and the length of the X-axis guide rail 211 is adapted to the length of the X-axis synchronous belt transmission mechanism 204. The cantilever arm 205 is fixedly connected to an X-axis slider 212 slidably mounted on the X-axis guide rail 211, so that the X-axis guide rail 211 stably clamps the cantilever arm 205 and guides the movement of the cantilever arm 205; in addition, since the extended length of the cantilever arm 205 is relatively long, in order to prevent the front end of the cantilever arm 205 from falling, the height of the cantilever arm 205 is set to be consistent with the height of the connecting beam of the support frame 201, and a support slide 213 is horizontally opened on the inner side surface of the connecting beam on the front side of the support frame 201, and a support slide 214 is provided at the front end of the cantilever arm 205, which is slidably matched with the support slide 213, so as to form a stable support for the front end of the cantilever arm 205.

[0055] In order to ensure that the sample loading arm 208 moves stably with the Y-axis synchronous belt transmission mechanism 207, a Y-axis guide rail 215 is provided on the cantilever arm 205, and the length of the Y-axis guide rail 215 is adapted to the length of the Y-axis synchronous belt transmission mechanism 207. The sample loading arm 208 is fixedly connected to a Y-axis slider 216 slidably mounted on the Y-axis guide rail 215, so that the Y-axis guide rail 215 can stably fix the sample loading arm 208 and guide the movement of the sample loading arm 208.

[0056] In order to ensure that the sample loading gun moves stably with the Z-axis synchronous belt transmission mechanism 210, a Z-axis guide rail 217 is provided on the sample loading arm 208, and the length of the Z-axis guide rail 217 is adapted to the length of the Z-axis synchronous belt transmission mechanism 210. The sample loading gun is fixedly connected to a Z-axis slider 218 slidably mounted on the Z-axis guide rail 217, so that the Z-axis guide rail 217 stably fixes the sample loading gun and guides the up and down movement of the sample loading gun.

[0057] like Figure 7 As shown, the sample adding gun includes a liquid suction pump motor 219 fixed on the synchronous belt of the Z-axis synchronous belt transmission mechanism 210, the working shaft of the liquid suction pump motor 219 is vertically downward, and a synchronously rotating transmission screw 220 is fixedly connected to its lower end through a coupling, and a screw nut 221 is provided on the transmission screw 220, which moves up and down along its length direction, and a piston-type liquid suction pump gun 222 (the piston-type liquid suction pump gun 222 is a common automatic liquid adding gun in the prior art) fixedly connected to the housing of the liquid suction pump motor 219 is vertically downwardly provided below the transmission screw 220 , which can realize accurate sample suction and accurate sample addition), the upper piston rod of the piston-type liquid suction pump gun 222 is fixedly connected with the screw nut 221, and the transmission screw 220 is driven to rotate by the liquid suction pump motor 219, so that the screw nut 221 arranged thereon moves up and down, realizing the up and down push and pull of the piston rod of the piston-type liquid suction pump gun 222 to complete the liquid suction or sample addition action; a disposable liquid suction gun head 223 is movably mounted on the liquid suction port at the lower end of the piston-type liquid suction pump gun 222, and the disposable liquid suction gun head 223 is detachable and replaceable, which effectively avoids cross contamination.

[0058] The microplate supporting device is generally fixed on the inner bottom wall of the whole machine housing 1 at the center of the supporting frame 201; Figure 8 , 9As shown, the microplate supporting device includes a supporting base 224, on which a rotating main shaft 226 driven by a substrate driving motor 225 is vertically penetrated. In order to make the structure more compact, the substrate driving motor 225 is fixed on the lower surface of the supporting base 224, and drives the rotating main shaft 226 through a set of synchronous belt transmission mechanism; the upper end of the rotating main shaft 226 is fixedly connected to the center of the bottom surface of the horizontally arranged supporting substrate 227; in order to improve the flexibility and stability of the microplate supporting device during operation, a pair of bearings (preferably deep groove bearings) are mounted on the rotating main shaft 226 close to the supporting base 224, and a shaft sleeve that is tightly matched with the outer circumference of the bearing is fixedly installed on the supporting base 224 by bolts, and an external thread is opened on the rotating main shaft 226 below the bearing, on which a stop gasket and a locking nut are arranged in sequence. Two sets of microplate supporting assemblies are fixedly arranged side by side on the supporting base plate 227, and each set of microplate supporting assemblies includes a set of microplate brackets 228 and a set of plate cover clamping frames 229. The height of the plate cover clamping frames 229 is higher than the height of the microplate brackets 228, so that the plate cover of the microplate is first clamped on the higher plate cover clamping frame 229, and then the microplate is withdrawn downward and clamped on the microplate bracket 228; the microplate brackets 228 and the plate cover clamping frames 229 on the two sets of microplate supporting assemblies are staggered, so that the sample loading area has two stations (that is, two microplates can be placed). When the microplate on one station is loaded with samples, the supporting base plate 227 is driven by the base plate driving motor 225 to rotate 180°, and the microplate on the other station can be loaded with samples, which can effectively save the loading time and improve work efficiency. In addition, a sample loading origin position sensor 230 and a sample loading graduation position sensor 231 are arranged on the upper surface of the supporting base 224, and an origin position baffle 232 adapted to the sample loading origin position sensor 230 and a graduation position baffle 233 adapted to the sample loading graduation position sensor 231 are arranged on the lower surface of the supporting base plate 227. The position of the microplate is identified and recorded in real time through the cooperation between the sample loading origin position sensor 230 and the origin position baffle 232, and the sample loading graduation position sensor 231 and the graduation position baffle 233, thereby realizing position memory and facilitating automatic loading.

[0059] like Figure 3 , 4 As shown, the gun tip loading and unloading device includes a gun tip discarding bin 234 disposed at the front side of the microplate supporting device, and a discarded gun tip storage bucket 235 (such as Figure 1As shown in the figure, a gun tip dropping opening 236 is provided on the top wall of the gun tip discarding bin 234, and a support table 238 with a gun tip holding box 237 on the upper surface is provided above the gun tip dropping opening 236. A plurality of disposable liquid-pipette gun tips 223 are placed in the gun tip holding box 237. The sample adding gun is aimed at a disposable liquid-pipette gun tip 223 in the gun tip holding box 237 and then moves downward to complete the installation of the disposable liquid-pipette gun tip 223; a gourd-shaped gun tip removing opening 239 corresponding to the gun tip dropping opening 236 is provided on the support table 238 to facilitate the removal of the used disposable liquid-pipette gun tip 223, and the disposable liquid-pipette gun tip 223 after removal directly falls into the discarded gun tip storage bucket 235.

[0060] The sample adding gun will perform the liquid aspiration operation after the installation of the disposable liquid aspiration gun head 223 is completed. Therefore, it is necessary to scan and record multiple sample bottles containing sample liquid first, and then the corresponding sample bottle can be automatically selected when aspirating the sample liquid. The sample identification and recording unit is set to complete this operation.

[0061] like Fig.10 , 11 As shown, the sample identification and recording unit 3 includes a sample support seat 301 arranged below the movement trajectory of the sample loading arm 208. Specifically, the sample support seat 301 is arranged on one side of the gun head loading and unloading device to shorten the movement stroke of the sample loading gun during liquid aspiration as much as possible; a sample tray rotating shaft 303 driven by the identification and recording unit motor 302 is vertically penetrated on the sample support seat 301. To make the structure more compact, the identification and recording unit motor 302 is fixed to the lower surface of the sample support seat 301, and the sample tray rotating shaft 303 is also driven by a set of synchronous belt transmission mechanism. The upper end of the sample tray rotating shaft 303 is horizontally fixedly connected with a sample bottle tray connecting plate 304. To ensure stability during operation, an external thread is also provided on the sample tray rotating shaft 303 and the shaft sleeve is screwed. A stop gasket and a locking nut are sequentially provided on the sample tray rotating shaft 303 located at the lower end of the shaft sleeve to prevent the sample tray rotating shaft 303 from loosening and falling off after long-term use.

[0062] A ring tray 305 is movably placed on the sample bottle tray connecting plate 304, and a plurality of slots 307 for inserting sample bottles 306 are evenly distributed along the circumference of the ring tray 305. Fig.12 As shown, a product factory barcode 308 and a patient information barcode 309 are respectively pasted on the inner and outer side walls of the sample bottle 306; barcode scanners 310 corresponding to the positions of the product factory barcode 308 and the patient information barcode 309 on the same sample bottle 306 are respectively fixed on the sample support seats 301 inside and outside the ring tray 305. The two barcode scanners 310 scan the same sample bottle 306 at the same time, with a fast scanning speed and greatly improved work efficiency.

[0063] An annular code disk 311 is fixedly connected to the lower surface of the sample bottle tray connecting plate 304, and a code disk origin position sensor 312 and a code disk graduation position sensor 313 are provided on the sample support seat 301 located on one side of the annular code disk 311. The code disk origin position sensor 312 and the code disk graduation position sensor 313 are used to identify and record the position of the sample bottle 306 in real time, thereby realizing position memory. Therefore, there is no need to consider the insertion position when placing the sample bottle 306, which is convenient for the operator to place it randomly and greatly reduces the difficulty of operation.

[0064] When loading the plate after the sample gun has aspirated the liquid, you also need to first place the microplate (such as Fig.15 As shown in the figure, the microplate is pushed onto the microplate holding device, so the plate holding unit 4 is arranged on one side of the microplate holding device.

[0065] like Fig.13 , 14 As shown, the carrier unit 4 includes a fixed base 401, on which a board release mechanism is fixedly connected through a pair of longitudinal support plates 402 arranged at intervals; in order to enhance the supporting capacity of the longitudinal support plates 402, one side of the two longitudinal support plates 402 is connected as a whole through a reinforcing rib plate 403 installed on the fixed base 401.

[0066] The board release mechanism includes a bottom cover 404 fixedly mounted horizontally on two longitudinal support plates 402, and a board release port 405 is provided on the bottom cover 404. The board release port 405 is a rectangular structure similar to the structure of the existing micro-well plate 406, and its size is slightly larger than the size of the existing micro-well plate 406, so as to ensure that the micro-well plate 406 can smoothly fall from the board release port; an opening and closing plate with a rectangular ring structure is movably mounted at the upper edge of the board release port 405 through a guide pin 407 fixed on the bottom cover 404; the guide pin 407 is evenly arranged along the edge of the board release port 405. The opening and closing plate is formed by a pair of U-shaped thin plates 408 that move in opposite directions. The joints of the two U-shaped thin plates 408 are connected as a whole through a rotating sleeve 409, so that the U-shaped thin plate 408 on one side moves, driving the U-shaped thin plate 408 on the other side to move in the opposite direction, thereby realizing the opening and closing function; strip holes 410 that are compatible with the guide pins 407 are opened on the two U-shaped thin plates 408, and the length direction of the strip holes 410 is consistent with the movement direction of the U-shaped thin plates 408. The guide pins 407 are movably mounted in the strip holes 410 to realize the guiding effect on the guide pins 407.

[0067] The two U-shaped thin plates 408 are an integrated structure consisting of two side wall thin plates and a bottom wall thin plate. A group of mutually symmetrical claws 411 are arranged on the opposite sides of the bottom wall thin plates of the two U-shaped thin plates 408 for clamping the microporous plate 406. A return spring 412 is arranged on one of the U-shaped thin plates 408, and the outer end of the return spring 412 is connected to the bottom cover 404. The stretching direction of the return spring 412 is consistent with the opening and closing direction of the U-shaped thin plates 408, so as to ensure that the two U-shaped thin plates 408 in the open state are closed under the action of the return spring 412. A plate release motor 413 is fixed on the lower surface of the bottom cover 404, and the plate release motor 413 works A board release screw 414 is coaxially fixed to the shaft, and the axial direction of the board release screw 414 is consistent with the opening and closing direction of the two U-shaped thin plates 408. A nut seat 415 is provided on the board release screw 414 to drive the two U-shaped thin plates 408 to open and close, and a pair of stroke position sensors 416 for monitoring the movement stroke of the nut seat 415 are arranged at intervals on the board release motor 413; the nut seat 415 is connected to the U-shaped thin plate 408 on one side to drive it to complete the opening and closing action, and the two stroke position sensors 416 arranged at intervals are used to monitor the position of the nut seat 415 and limit the maximum stroke of the nut seat 415.

[0068] A connecting base 417 fixedly connected to the bottom cover 404 is buckled above the two U-shaped thin plates 408, and a board and card dropping opening 418 consistent with the board and card releasing opening 405 is provided on the connecting base 417, and a U-shaped through groove 420 corresponding to the position of the information barcode 419 on the microplate 406 is provided on one side of the board and card dropping opening 418, which is used to cooperate with its external scanning mechanism to scan the microplate 406 that is about to fall, and store and record the information.

[0069] A board stacking mechanism is provided on the board release mechanism, and the board stacking mechanism includes blocking columns 421 vertically fixed at the four corners of the board drop opening 418, and the upper ends of the blocking columns 421 are connected as a whole by connecting the top plate 422 to form a board stacking bin 423 adapted to the microplate 406, and a magnetic suction door 425 with a handle 424 is hinged on one side of the board stacking bin 423. A Hall sensor is installed. When the magnetic suction door 425 is not completely closed, the Hall sensor will send out an alarm signal to indicate that the magnetic suction door 425 is not closed properly. In addition, a convex strip 426 for fixing the microporous plate 406 is longitudinally arranged on the inner side surface of the magnetic suction door 425. When the magnetic suction door 425 is closed, the convex strip 426 can fix the microporous plate 406 located in the board and card stacking bin 423 to prevent it from moving left or right in the board and card stacking bin 423.

[0070] The microplate 406 is directly stacked horizontally in the card stacking bin 423. To adapt to the fool-proof function of the microplate 406 (i.e., one corner of the microplate 406 is a right angle, and the other three corners are arc angles, generally, when the microplate 406 is placed in the forward direction, its lower right corner is a right angle), the inner side surfaces of the two blocking columns 421 away from the magnetic suction door 425 are arc structures that match the microplate 406; at the same time, a card drop opening 418 located below the magnetic suction door 425 is provided at the edge thereof. Manual grabbing groove 427, when an error occurs in the microplate 406 at the bottom layer, the microplate 406 can be manually taken out through the manual grabbing groove 427; in addition, in order to facilitate clear observation of the number of boards and cards of the stacked microplates 406, a board and card number mark 428 corresponding to the microplate 406 is arranged from bottom to top on the outer side surface of one of the blocking columns 421, and the distance between the two adjacent board and card number marks 428 is the thickness of a microplate 406.

[0071] After the microplate 406 has been loaded with samples by the loading unit 2, it will enter the incubation unit 5 to incubate the loaded plate.

[0072] like Figure 16-18 As shown, the incubation unit 5 includes an incubation box 501 arranged on one side of the sample loading unit 2, an incubation base 502 is arranged in the incubation box 501, a thin-walled bearing 503 is horizontally arranged on the upper surface of the incubation base 502, the inner ring of the thin-walled bearing 503 is fixedly connected to the incubation base 502, and a driven pulley 505 driven by an incubation motor 504 is fixedly sleeved on the outer ring thereof, the upper surface of the driven pulley 505 is fixedly connected to a coaxially arranged horizontal incubation tray 506, eight groups of incubation towers 507 are evenly distributed along the circumference of the horizontal incubation tray 506, and eight layers of microplate slots 508 are longitudinally spaced in the incubation towers 507, a total of sixty-four microplates 406 can be placed at one time, which greatly increases the plate loading capacity. To facilitate the placement and removal of the incubation towers 507, each group of incubation towers 507 is fixed to the upper surface of the horizontal incubation tray 506 by pins, and a handle 509 is provided on the upper surface of the incubation towers 507. In addition, to facilitate the quick and accurate location of the required microplate 406 when placing and removing the microplate 406, a tower scale 510 corresponding to the incubation towers 507 is provided along the circumference of the upper surface of the horizontal incubation tray 506, and a position sensor 511 is provided on the incubation base 502 to record the position information of the rotation of the horizontal incubation tray 506 to ensure the rotation accuracy of the tower position.

[0073] In order to allow eight groups of incubation towers 507 with the same structure to be evenly distributed on the horizontal incubation tray 506, the diameter of the horizontal incubation tray 506 will be relatively large, resulting in a larger outer diameter of the driven pulley 505 that drives it to rotate, and most of the existing motors have a fixed speed. Therefore, in order to adapt to the speed of the incubation motor 504, a transmission 512 is provided on the incubation base 502, and the incubation motor 504 is connected to the driven pulley 505 through the transmission 512; specifically, the transmission 512 is fixedly connected to the lower surface of the incubation base 502 through a fixed plate 513, and the output end of the transmission 512 extends vertically upward to the top of the incubation base 502, and a driving pulley 514 is provided thereon, and the driving pulley 514 is connected to the driven pulley 505 through a transmission belt, and the input end of the transmission 512 is connected to the incubation motor 504 through a synchronous belt drive mechanism.

[0074] A plate inlet and a plate outlet 515 are provided on the side wall of the incubator 501. In order to cooperate with the sample loading unit and facilitate the automatic placement of the microplate 406, the plate inlet is composed of eight plate inlet door holes 516 opened at longitudinal intervals. The eight plate inlet door holes 516 correspond one to one to the eight-layer microplate slots 508. A push door 518 is hinged on the plate inlet door hole 516 through an automatic rebound hinge 517; the automatic rebound hinge 517 is a hydraulic hinge (the full name is a torsion spring type hydraulic buffer door closing hinge) or a pneumatic hinge (the full name is a pneumatic automatic door closing hinge), which ensures that when the microplate 406 is sent in and then exits the workstation, the push door 518 rebounds and the plate inlet door hole 516 is closed, thereby realizing automation and reducing the risk of failure caused by unstable factors.

[0075] The plate outlet 515 is sealed and connected to the front side wall of the whole machine housing 1. The size of the plate outlet 515 is adapted to the size of the outer opening of a group of incubation towers 507. An incubator door 519 is hinged on the front side wall of the whole machine housing 1 corresponding to the plate outlet 515. To enhance the sealing performance, an annular sealing strip that fits the edge of the plate outlet 515 is provided on the inner side of the incubator door 519; a magnetic seat that attracts the plate outlet 515 may also be provided on the opening side of the incubator door 519, and a magnetic seat may be provided on the plate outlet near the magnetic seat. Hall sensor, when the incubator compartment door 519 is not completely closed, the Hall sensor will send out an alarm signal, indicating that the incubator compartment door 519 is not closed properly; in addition, a number of incubation information indicator lights 520 are arranged on the edge of the outlet port 515 corresponding to the opening side of the incubator compartment door 519. The incubation information indicator lights 520 correspond one-to-one to a group of microplate slots 508 on an incubation tower 507. The incubation information indicator lights 520 can be displayed in different colors through relevant software, corresponding to different incubation information of the microplate 406 in the incubation tower 507.

[0076] A heating fan 522 with a heating wire 521 is arranged on the top wall of the incubator 501, and a temperature sensor is arranged in the incubator 501 for real-time monitoring and feedback of temperature information in the incubator 501; a warming information display screen 523 is arranged on the front side wall of the whole machine shell 1 located below the incubator compartment door 519, which can display warming information at any time, so as to facilitate the real-time parameters in the incubator 501 to be grasped at any time; in addition, a thermometer 524 is sealed and penetrated on the top wall of the incubator 501, and the upper end of the thermometer 524 passes through the top wall of the whole machine shell 1, so that the operator can read the temperature information intuitively.

[0077] After the board is incubated in the incubation unit 5, it will enter the detection unit 6 for identification and drug sensitivity testing.

[0078] like Figure 19-21 As shown, the detection unit 6 includes a detection base 601 arranged at the inner center of the incubation tower 507, a first guide rail 602 is longitudinally arranged on the detection base 601, an information collection mechanism driven to rise and fall by a first power mechanism 603 is slidably arranged on the first guide rail 602, the first power mechanism 603 is a synchronous belt transmission mechanism arranged on the detection base 601, and a counterweight mechanism is arranged on the detection base 601 to prevent the information collection mechanism from falling, so as to avoid the collection mechanism from falling suddenly, and has a protective effect.

[0079] The information collection mechanism includes a horizontal lifting frame 604 slidably arranged on the first guide rail 602, a second guide rail 605 is horizontally arranged in the horizontal lifting frame 604 close to the long side wall, a slider 607 driven by a second power mechanism 606 is slidably arranged on the second guide rail 605, a tray connecting block 608 is horizontally fixedly connected to one side of the slider 607, a detection tray 609 is fixedly connected to the side of the tray connecting block 608 away from the slider 607, a clamping hole 610 adapted to the bottom surface of the microplate 406 is opened on the detection tray 609, and is used to clamp the microplate 406, and the detection tray 609 is opened from the tray inlet and outlet 610 opened on the short side wall of the horizontal lifting frame 604 1 passes through; an optical fiber fixing block 612 is arranged at the lower edge of the tray inlet and outlet 611, and an LED lamp 613 is arranged at the bottom of the horizontal lifting frame 604. The light emitted by the LED lamp 613 is filtered by the filter and irradiated into the optical fiber fixing block 612 by the optical fiber 614. The light beam coming out of the optical fiber fixing block 612 is then irradiated on the plate hole of the microplate 406 through the detection tray 609, and finally collected by the photoelectric signal conversion module 615 covering the top of the horizontal lifting frame 604, and the collected attenuated optical signal is transmitted to the single-chip microcomputer (i.e., PLC controller), and the drug resistance parameter of the sample is calculated by the single-chip microcomputer to complete the information collection and detection.

[0080] The counterweight mechanism includes a gate-shaped frame 616 vertically placed on the detection base 601, the first guide rail 602 and the information collection mechanism are located on the inner side of the gate-shaped frame 616, and a third guide rail 617 is longitudinally arranged on the detection base 601 behind the gate-shaped frame 616. A hanging shaft 618 located above the photoelectric signal conversion module 615 is installed on the horizontal lifting frame 604. The hanging shaft 618 is horizontally arranged along the width direction of the gate-shaped frame 616. A steel cable 619 with one end fixed to the hanging shaft 618 passes around the fixed pulley 620 at the top of the gate-shaped frame 616 and is fixedly connected to a counterweight block 621 slidably arranged on the third guide rail 617. A shock-absorbing pad 622 is mounted on the lower end of the third guide rail 617. The shock-absorbing pad 622 is located under the counterweight block 621 and is adapted to correspond to it to cushion the falling of the counterweight block 621.

[0081] In addition, in order to improve the stability of the information collection mechanism during movement, a first screw rod 623 extending vertically upward is fixedly connected to the passive wheel of the first power mechanism 603, and a lifting nut seat 624 is arranged on the side opposite to the tray inlet and outlet 611 of the horizontal lifting frame 604, and the lifting nut seat 624 is connected to the first nut screwed on the first screw rod 623; the second power mechanism 606 is a synchronous belt transmission mechanism arranged on the horizontal lifting frame 604, and a second screw rod 625 parallel to the second guide rail 605 is fixedly connected to the passive wheel of the second power mechanism 606, and a second nut 626 is screwed on the second screw rod 625, and the slider 607 is a nut slider connected to the second nut 626, and a first position sensor 627 is arranged on the horizontal lifting frame 604 located at the rear end of the second screw rod 625, and a sensing sheet 628 is arranged on the slider 607. When the first position sensor 627 detects the signal of the sensing sheet 628, it indicates that the detection tray 609 is retracted to the limit position; an L-shaped connecting piece 629 (the connecting piece 629 is a sheet metal part) is arranged on the horizontal lifting frame 604 located at the front end of the second screw rod 625, and a second position sensor 630 is arranged on the connecting piece 629 for sensing whether the microplate 406 is stuck on the detection tray 609.

[0082] The board transfer unit serves as a connection mechanism between the board carrier unit 4 , the sample loading unit 2 and the incubation unit 5 , transfers the board from the board carrier unit 4 to the sample loading unit 2 , and transfers and pushes the board after sample loading in the sample loading unit 2 into the incubation unit 5 .

[0083] like Fig. 22 , 23As shown, the board transfer unit 7 includes a longitudinal base plate 701 fixedly attached to the front side of the back plate 202, a transverse transmission belt 703 driven by a transverse driving motor 702 is horizontally arranged on the front side of the longitudinal base plate 701, and a plurality of arm plate position sensors 704 are arranged at intervals along the length direction of the longitudinal base plate 701 located below the transverse transmission belt 703, the conveying end of the transverse transmission belt 703 is adapted to correspond to the board inlet of the incubator 501, and a longitudinal arm plate 705 is fixedly arranged on the transverse transmission belt 703; a longitudinal driving motor 706 is arranged at one end of the longitudinal arm plate 705, and the end of the working shaft of the longitudinal driving motor 706 is connected to the incubator 501 by a coupling. There is a synchronous rotating screw 707 located on the front side of the longitudinal arm plate 705, the axial direction of the synchronous rotating screw 707 is consistent with the length direction of the longitudinal arm plate 705, and an up and down sliding screw nut 708 is arranged on the synchronous rotating screw 707. A height positioning plate consistent with the length direction of the synchronous rotating screw 707 is arranged on the longitudinal arm plate 705, and a microplate transfer support bracket and a nut position sensor 709 compatible with the height positioning plate are arranged on the screw nut 708. The length of the height positioning plate should match the movement distance of the screw nut 708 to ensure that the position information of the vertical movement of the screw nut 708 can be monitored throughout the entire stroke of the screw nut 708.

[0084] In order to improve the stability of the longitudinal arm plate 705 when it moves horizontally along the longitudinal base plate 701, arm plate guide rails 710 that are consistent with the length direction of the horizontal transmission belt 703 are symmetrically arranged on the front side surfaces of the longitudinal base plate 701 located on the upper and lower sides of the horizontal transmission belt 703. The length of the wall plate guide rails 710 should be compatible with the length of the horizontal transmission belt 703, and the longitudinal arm plate 705 is fixedly connected to an arm plate slider 711 that is slidably mounted on the arm plate guide rails 710; in addition, arm plate limiting columns 712 for blocking the longitudinal arm plate 705 are respectively arranged on the longitudinal base plate 701 at both ends of the horizontal transmission belt 703, which can effectively avoid the risk of derailment of the longitudinal arm plate 705 when the horizontal drive motor 702 fails to position (that is, when the horizontal drive motor 702 fails), and the arm plate limiting columns 712 are screws fixed on the longitudinal base plate 701, which are convenient for material acquisition.

[0085] In order to improve the stability of the lead screw nut 708 when it moves vertically along the longitudinal arm plate 705, a nut guide rail 713 is provided on the front side of the longitudinal arm plate 705 in the same length direction as the synchronously rotating lead screw 707. The length of the nut guide rail 713 should be adapted to the movement stroke of the lead screw nut 708. The lead screw nut 708 is fixedly connected to a lead screw connecting slider slidably arranged on the nut guide rail 713. Nut limiting columns 714 for blocking the lead screw nut 708 are respectively provided on the longitudinal arm plates 705 at both ends of the synchronously rotating lead screw 707, which can effectively avoid the risk of the lead screw nut 708 derailing when the longitudinal drive motor 706 fails to position (i.e., when the longitudinal drive motor 706 fails).

[0086] like Fig.24 As shown, the microplate transfer support bracket includes a board transfer cantilever arm 715 fixedly connected to the screw nut 708 and extending horizontally forward, a board transfer bracket 716 matching the board entry door 516 is horizontally fixedly connected to the board transfer cantilever arm 715, and a bracket in-place sensor 717 is provided on the side of the board transfer bracket 716 to monitor in real time whether the microplate 406 is delivered to the position. In addition, a buffer rubber block 718 for blocking the microwell plate 406 is arranged on the side of the board transfer bracket 716 away from the board transfer cantilever arm 715, which plays a buffering role when the microwell plate 406 moves horizontally along the longitudinal substrate 701; the upper surface of the board transfer bracket 716 is evenly distributed with 10×12 microwell plate positioning holes 719 that are adapted to the bottom surface of the plate holes of the microwell plate 406, and manual grabbing grooves 720 are respectively opened on both sides of the board transfer bracket 716 between the microwell plate positioning holes 719 and the board transfer cantilever arm 715, which are used to avoid interference from other components when taking and placing the microwell plate 406.

[0087] In addition, in order to prevent the internal cables from affecting the operation of the entire system, a drag chain 8 is provided on the motion trajectory of the X-axis synchronous belt drive mechanism 204, the Y-axis synchronous belt drive mechanism 207 and the Z-axis synchronous belt drive mechanism 210 of the loading unit, on the motion trajectory of the first power mechanism 603 and the second power mechanism 606 of the detection unit, and on the motion trajectory of the transverse transmission belt 703 of the board transfer unit and the microplate transfer support bracket. The cables are hidden in the drag chain 8 to form an effective protection to prevent interference with other moving parts or line wear.

[0088] The working process of the present invention is as follows:

[0089] First, the plate carrier unit 4 will scan the microplate 406 in the plate card release mechanism, and at the same time, the sample identification and recording unit 3 will scan the sample bottle 306 placed on the ring tray 305. The system will automatically match the category information of the microplate 406 and the sample bottle. After the match is successful, the plate card transfer unit will take out the microplate 406 from the plate card stacking mechanism and transfer it to the microplate supporting device, and at the same time complete the opening action of the microplate 406; then the system will automatically select the corresponding sample bottle 306 according to the category information of the microplate 406, and transfer the sample bottle 306 to the side close to the gun head loading and unloading device; then the sample adding gun of the sample adding unit 2 will operate to complete the installation of the disposable liquid pipette gun head 223, and after the installation is completed, the disposable liquid pipette gun head 223 will be installed. After the disposable pipette tip 223 is used, the sample adding gun moves to the sample bottle 306 to be aspirated to aspirate; then the sample adding gun moves to the microplate supporting device to add liquid; after the sample adding is completed, the board transfer unit 7 lifts up the microplate 406 with the sample added, and transports it to the incubator 501 for incubation. At the same time, the sample adding gun will move to the gun tip loading and unloading device, unload the used disposable pipette tip 223, and wait for the next installation; after the incubation is completed, the detection unit 6 will take the microplate 406 away from the inner side of the incubation tower 507 for identification and drug sensitivity testing, and put it back to the incubation tower 507 after the test, and reflect the detected data to the computer in real time, completing a whole set of actions.

Claims

1. A fully automatic microbial identification and drug sensitivity analysis system, characterized in that: It comprises a whole machine housing, in the inner cavity of which are arranged a sample adding unit, a sample identification and recording unit, a plate carrying unit, an incubation unit, a detection unit and a plate card transfer unit; The loading unit automatically completes loading and unloading of gun tips, gun body liquid suction and board loading, and comprises a support frame installed in the whole machine housing, and a back plate fixedly arranged longitudinally between two upright posts on the rear side of the support frame; an X-axis synchronous belt transmission mechanism driven by an X-axis motor is horizontally arranged on the rear side of the back plate, a cantilever arm extending horizontally forward is fixedly connected to the synchronous belt of the X-axis synchronous belt transmission mechanism, a Y-axis synchronous belt transmission mechanism driven by a Y-axis motor is horizontally arranged on the cantilever arm, a loading arm extending vertically downward is fixedly connected to the synchronous belt of the Y-axis synchronous belt transmission mechanism, a Z-axis synchronous belt transmission mechanism driven by a Z-axis motor is longitudinally arranged on the loading arm, and a loading gun with a liquid suction port vertically downward is fixedly connected to the synchronous belt of the Z-axis synchronous belt transmission mechanism; a microplate supporting device and a gun tip loading and unloading device are arranged below the motion track of the loading arm; The sample identification and recording unit is located at one side of the sample loading unit, and scans, records and automatically selects the sample bottles containing the samples. The sample identification and recording unit includes a sample support seat arranged below the motion track of the sample loading arm, and a sample tray rotating shaft driven by the identification and recording unit motor is vertically penetrated on the sample support seat, and a sample bottle tray connecting plate is horizontally fixedly connected to the upper end of the sample tray rotating shaft, and an annular tray is movably placed on the sample bottle tray connecting plate, and a plurality of card slots for inserting and placing sample bottles are evenly distributed along the circumference of the annular tray, and the product factory barcode and the patient information barcode are respectively pasted on the inner and outer side walls of the sample bottle; barcode scanners corresponding to the positions of the product factory barcode and the patient information barcode on the same sample bottle are respectively fixed on the sample support seats inside and outside the annular tray; an annular code disk is fixedly connected to the lower surface of the sample bottle tray connecting plate, and a code disk origin position sensor and a code disk graduation position sensor are arranged on the sample support seat located on one side of the annular code disk; The plate loading unit is located at the other side of the sample loading unit and is used for loading and scanning recording plates and cards; The incubation unit is used to incubate the sample-added board; The detection unit performs identification and drug sensitivity test on the incubated board and card; The board transfer unit serves as a connection mechanism between the board carrier unit, the sample loading unit and the incubation unit, and transfers the board from the board carrier unit to the sample loading unit, and then transfers and pushes the board from the sample loading unit to the incubation unit; Among them, the board carrier unit scans the board card, and the sample identification and recording unit scans the sample bottle placed on the ring tray. The system automatically matches the category information of the board card and the sample bottle. After the match is successful, the board card transfer unit takes out the board card from the board carrier unit; then the system automatically selects the corresponding sample bottle according to the category information of the board card; then the sample loading unit aspirates the selected sample bottle and adds it to the board card; after the sample loading is completed, the board card transfer unit lifts the board card with sample loading and transports it to the incubation unit for incubation; after the incubation is completed, the detection unit takes the board card away from the inner side of the incubation unit for identification and drug sensitivity testing. After the test, the board card is put back to the original position of the incubation unit, and the detected data is reflected on the computer in real time.

2. The fully automatic microbial identification and drug sensitivity analysis system according to claim 1, characterized in that: The microplate supporting device comprises a supporting base, on which a rotating spindle driven by a substrate driving motor is vertically penetrated, and the upper end of the rotating spindle is fixedly connected to the center of the bottom surface of a horizontally arranged supporting substrate; two sets of microplate supporting assemblies are fixedly arranged side by side on the supporting substrate, and each set of the microplate supporting assemblies comprises a set of microplate brackets and a set of plate cover clamping frames, the height of the plate cover clamping frames is higher than the height of the microplate brackets, and the microplate brackets and plate cover clamping frames on the two sets of microplate supporting assemblies are arranged alternately; The gun tip loading and unloading device comprises a gun tip discarding bin arranged at the front side of the microplate supporting device, a storage bucket for discarded gun tips drawn out or put in from the front side of the whole machine housing is arranged in the gun tip discarding bin, a gun tip drop-off opening is provided on the top wall of the gun tip discarding bin, a support table with a gun tip storage box on the upper surface is provided above the gun tip drop-off opening, and a gourd-shaped gun tip removal opening corresponding to the gun tip drop-off opening is provided on the support table; The sample adding gun includes a liquid suction pump motor fixed on the synchronous belt of the Z-axis synchronous belt transmission mechanism, the working shaft of the liquid suction pump motor is vertically downward, and a synchronously rotating transmission screw is fixedly connected to its lower end through a coupling, and a screw nut that moves up and down along its length direction is arranged on the transmission screw, and a piston type liquid suction pump gun fixedly connected to the housing of the liquid suction pump motor is arranged vertically downward below the transmission screw, the upper end piston rod of the piston type liquid suction pump gun is fixedly connected to the screw nut, and a disposable liquid suction gun head located in the gun head holding box is movably mounted on the liquid suction port at its lower end.

3. The fully automatic microbial identification and drug sensitivity analysis system according to claim 1, characterized in that: The plate carrier unit comprises a fixed base arranged on one side of the microplate supporting device, and a plate release mechanism is fixedly connected to the fixed base through a pair of longitudinal support plates arranged at intervals; The board release mechanism comprises a bottom cover fixedly mounted horizontally on the two longitudinal support plates, a board release opening is formed on the bottom cover, and an opening and closing plate with a rectangular ring structure is movably mounted on the upper edge of the board release opening through a guide pin fixed on the bottom cover; the opening and closing plate is formed by a pair of U-shaped thin plates that move towards each other, and the butt joints of the two U-shaped thin plates are connected as a whole through a rotating sleeve, and a group of mutually symmetrical claws are arranged on opposite sides of the bottom wall thin plates of the two U-shaped thin plates, wherein a return spring with an outer end connected to the bottom cover is arranged on one of the U-shaped thin plates, and the stretching direction of the return spring is consistent with the opening and closing direction of the U-shaped thin plate; a A board and card release motor, a board and card release screw is coaxially fixedly connected to the working shaft of the board and card release motor, the axial direction of the board and card release screw is consistent with the opening and closing direction of the two U-shaped thin plates, a nut seat for driving the two U-shaped thin plates to open and close is arranged on the board and card release screw, and a pair of stroke position sensors for monitoring the movement stroke of the nut seat are arranged at intervals on the board and card release motor; a connecting base fixedly connected to the bottom cover is buckled above the two U-shaped thin plates, a board and card drop opening consistent with the board and card release opening is opened on the connecting base, and a U-shaped through groove corresponding to the position of the information barcode on the microplate is opened on one side of the board and card drop opening; A board and card stacking mechanism is arranged on the board and card releasing mechanism, and the board and card stacking mechanism includes blocking columns which are vertically fixed at the four corners of the board and card drop opening, and the upper ends of the blocking columns are connected as a whole by connecting the top plate to form a board and card stacking bin adapted to the microporous plate, and a magnetic suction door with a handle is hinged on one side of the board and card stacking bin, and a Hall sensor is installed on the blocking column which is attracted to the magnetic suction door.

4. The fully automatic microbial identification and drug sensitivity analysis system according to claim 1, characterized in that: The incubation unit comprises an incubation box arranged at one side of the sample loading unit, an incubation base is arranged in the incubation box, a thin-walled bearing is horizontally arranged on the upper surface of the incubation base, the inner ring of the thin-walled bearing is fixedly connected to the incubation base, and a driven pulley driven by the incubation motor is fixedly sleeved on the outer ring thereof, the upper surface of the driven pulley is fixedly connected to a coaxially arranged horizontal incubation tray, a plurality of groups of incubation towers with openings facing outward are uniformly distributed along the circumference on the horizontal incubation tray, a plurality of layers of microplate slots are longitudinally spaced in the incubation towers, and each group of incubation towers is fixed to the upper surface of the horizontal incubation tray by pins; A board inlet and a board outlet are provided on the side wall of the incubator, the board inlet is composed of a plurality of board inlet door holes longitudinally spaced apart, the board inlet door holes correspond one-to-one to the microplate slots, and a push door is hinged on the board inlet door hole through an automatic rebound hinge; the board outlet is sealed and connected to the front side wall of the whole machine shell, the size of the board outlet is adapted to the size of the outer opening of a group of incubation towers, and an incubator compartment door is hinged on the front side wall of the whole machine shell corresponding to the board outlet; a heating fan with a heating wire is provided on the top wall of the incubator box, a temperature sensor is provided in the incubator, and a temperature information display screen is provided on the front side wall of the whole machine shell below the incubator compartment door.

5. The fully automatic microbial identification and drug sensitivity analysis system according to claim 4, characterized in that: A transmission is arranged on the incubation base, and the transmission is fixedly connected to the lower surface of the incubation base through a fixing plate, and the output end of the transmission vertically extends upward to the top of the incubation base, and a driving pulley is arranged thereon, and the driving pulley is connected to the driven pulley through a transmission belt, and the input end of the transmission is connected to the incubation motor through a synchronous transmission mechanism; a plurality of incubation information indicator lights are arranged on the edge of the plate outlet corresponding to the opening side of the incubation box door, and the incubation information indicator lights correspond one-to-one to a group of microplate slots on the incubation tower.

6. The fully automatic microbial identification and drug sensitivity analysis system according to claim 4, characterized in that: The detection unit comprises a detection base arranged at the inner center of the incubation tower, a first guide rail is longitudinally arranged on the detection base, an information collection mechanism driven to rise and fall by a first power mechanism is slidably arranged on the first guide rail, and a counterweight mechanism is arranged on the detection base to prevent the information collection mechanism from falling; The information collection mechanism includes a horizontal lifting frame slidably arranged on the first guide rail, a second guide rail is horizontally arranged in the horizontal lifting frame close to the long side wall, a slider driven by a second power mechanism is slidably arranged on the second guide rail, a tray connecting block is horizontally fixedly connected to one side of the slider, a detection tray is fixedly connected to the side of the tray connecting block away from the slider, and the detection tray passes through the tray inlet and outlet opened on the short side wall of the horizontal lifting frame; an optical fiber fixing block is arranged at the lower edge of the tray inlet and outlet, an LED lamp is arranged at the bottom of the horizontal lifting frame, and the light emitted by the LED lamp is irradiated on the detection tray by the optical fiber through the optical fiber fixing block, and the top of the horizontal lifting frame is covered with a photoelectric signal conversion module.

7. The fully automatic microbial identification and drug sensitivity analysis system according to claim 6, characterized in that: The counterweight mechanism includes a gate-shaped frame vertically arranged on the detection base, the first guide rail and the information acquisition mechanism are located on the inner side of the gate-shaped frame, a third guide rail is longitudinally arranged on the detection base behind the gate-shaped frame, a hanging shaft located above the photoelectric signal conversion module is installed on the horizontal lifting frame, the hanging shaft is horizontally arranged along the width direction of the gate-shaped frame, and a steel cable with one end fixed on the hanging shaft passes around the fixed pulley on the top of the gate-shaped frame and is fixedly connected to the counterweight block slidably arranged on the third guide rail.

8. The fully automatic microbial identification and drug sensitivity analysis system according to claim 4, characterized in that: The board transfer unit includes a longitudinal base plate fixedly attached to the front side of the back plate, a transverse transmission belt driven by a transverse driving motor is horizontally arranged on the front side of the longitudinal base plate, a plurality of arm plate position sensors are arranged at intervals along the length direction of the longitudinal base plate located below the transverse transmission belt, the conveying end of the transverse transmission belt is adapted to correspond to the board inlet of the incubator, and a longitudinal arm plate is fixed on the transverse transmission belt; A longitudinal drive motor is arranged at one end of the longitudinal arm plate, and the end of the working shaft of the longitudinal drive motor is connected to a synchronously rotating lead screw located at the front side of the longitudinal arm plate through a coupling, the axial direction of the synchronously rotating lead screw is consistent with the length direction of the longitudinal arm plate, and a lead screw nut that slides up and down is arranged on the synchronously rotating lead screw, and a height positioning plate that is consistent with the length direction of the synchronously rotating lead screw is arranged on the longitudinal arm plate, and a microplate transfer support bracket and a nut position sensor that is compatible with the height positioning plate are arranged on the lead screw nut; The microplate transfer support bracket includes a board transfer cantilever arm fixedly connected to the screw nut and extending horizontally forward, a board transfer bracket matching the board entry door opening is horizontally fixedly connected to the board transfer cantilever arm, and a bracket in-place sensor is arranged on the side of the board transfer bracket.

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