Sample collection and detection device

Abstract

The present invention provides a device for collecting and detecting samples, including a first chamber for collecting liquid samples and a second chamber for collecting and confirming the detected liquid samples. The first chamber and the second chamber can be combined or separated. The device can separate the initial detection sample and the secondary confirmation detection sample. The initial samples collected by the detection device can enter the first chamber and the second chamber respectively. Then, after or before the initial detection is completed, the second chamber can be separated from the initial collection chamber to achieve one-time collection and secondary detection. There will be no contamination between the samples of the initial detection and the samples of the confirmation detection. The samples of the confirmation detection are sealed before the samples of the initial detection enter the detection area, ensuring that the samples of the secondary confirmation detection will not be contaminated by the detection liquid of the initial detection, thus affecting the effect of the secondary confirmation.

Description

Technical Field

[0001] The present invention relates to a device for collecting liquid samples, especially a device for collecting and detecting an analyte in a liquid sample in the field of rapid diagnosis, such as a urine collection and detection device. Background Art

[0002] Currently, detection devices for detecting whether a sample contains an analyte are widely used in hospitals or at home. These detection devices for rapid diagnosis include one or more test strips, such as early pregnancy detection, drug abuse detection, etc. Such rapid diagnosis detection devices are very convenient and can obtain test results on the test strip in one minute, or at most about ten minutes.

[0003] Drug detection is widely used in institutions such as anti-drug departments, public security bureaus, drug rehabilitation centers, physical examination centers, and national military recruitment physical examination sites. There are various types of drug detections and frequent detections. A urine cup for drug detection that can automatically separate the remaining sample from the detected sample has a huge market demand. Currently, after the drug detection urine cup on the market completes the detection, the sample in the urine cup will be contaminated by the test reagent and cannot be used for a second confirmation test, as described in U.S. Patent No. 7300633.

[0004] Although, in traditional technologies, the detected sample can be isolated from the collected sample, the cost is high and it is not easy to operate. For example, the piston urine cup described in U.S. Patent No. 7300633. When the piston is pushed forward, the liquid sample in the collection chamber, such as urine, is transferred from the collection chamber to the detection chamber. There is a test element in the detection chamber for detecting the analyte in the sample, and the liquid sample in the collection chamber is isolated by the piston, so that the samples in the two places will not be confused and can be used for subsequent confirmation tests. Although the detected sample and the collected sample can be isolated in this way, this piston urine cup has a high cost and is not easy to operate. After all, pushing the piston requires a relatively large amount of force because the piston needs to achieve sample transfer and must have a liquid sealing effect with the wall of the piston. To achieve the sealing effect, the piston and the piston chamber need to be closely combined.

[0005] For another example, U.S. Patent No. 8992855 describes a device for collecting liquid samples. The device includes a piston structure integrated with the lid and moving together with the lid. Although the detected sample and the collected sample can be separated, when the detected sample enters the detection chamber, it needs to overcome a large pressure to enter, and the dimensions of the lid and the cup mouth need to be precisely designed so that the piston integrated with the lid can accurately insert into the separation chamber.

[0006] In addition, after the initial tests of these traditional collection and detection devices are completed, if subsequent confirmation tests are required, the entire collection and detection device needs to be transported to a confirmation testing institution for further confirmation testing. This brings many problems, at least the following: First, most current liquid collection and detection devices only have a preliminary detection chamber. When subsequent confirmation testing is needed, only the entire device containing urine and the test strip can be sent to the confirmation testing institution for testing. The sample in the urine cup may be contaminated by the test reagent. Second, when sending the entire device to the confirmation testing institution, due to the relatively large cup mouth, there is a risk of liquid leakage during transportation. This requires more cost to make the device have a better sealing effect to minimize the risk of leakage. Third, after transporting the entire device to the confirmation testing institution, the confirmation testing institution needs a huge low-temperature warehouse to store the entire detection device to prevent the liquid sample from deteriorating, in preparation for possible further confirmation testing in the future. This causes a significant increase in the cost of the confirmation testing institution (which can be called the secondary testing institution).

[0007] In view of the above technical problems, it is necessary to improve them and provide an alternative way to solve the deficiencies of the existing traditional technologies. Summary of the Invention

[0008] In view of the above situation, to overcome the defects of the prior art, the technical problem to be solved by the present invention is to provide a sample collection and detection device that can separate the initial detection sample and the secondary confirmation detection sample. The initial sample collected by this detection device can enter the first chamber and the second chamber respectively. Then, after or before the initial detection is completed, the second chamber can be separated from the initial collection chamber (the first chamber) to achieve one-time collection and secondary detection. There will be no contamination between the initial detection sample and the confirmation detection sample. The confirmation detection sample is sealed before the initial detection sample enters the detection area, ensuring that the sample for secondary confirmation detection will not be contaminated by the detection liquid of the initial detection, thus affecting the effect of secondary confirmation.

[0009] To solve the above technical problems, the present invention provides the following:

[0010] In a first aspect, the present invention provides a device for collecting and detecting a sample. The sample detection device includes a first chamber for collecting a liquid sample and a second chamber for collecting and confirming the detected liquid sample. The first chamber and the second chamber can be combined or separated. That is to say, the combination of the first chamber and the second chamber can be a detachable combination, so as to ensure that the first chamber and the second chamber can be separated under certain conditions. In some preferred embodiments, the first chamber and the second chamber are combined in an initial state, and when the first chamber and the second chamber are in a combined state, they should be in fluid communication, that is, the liquid sample to be collected can enter the second chamber while entering the first chamber, or enter the first chamber while entering the second chamber, so as to achieve the above-mentioned one-time collection without separately collecting the first chamber and the second chamber. In some preferred ways, the first chamber has an external opening, and the opening of the second chamber is in communication with the interior of the first chamber.

[0011] Since there is a process of changing from combination to separation during the use of the first chamber and the second chamber, in some preferred ways, the outer shape of the second chamber needs to be designed to be able to partially separate from the first chamber. For example, a part of the second chamber can be exposed outside the outline of the first chamber. Further, in some preferred ways, as the second chamber and the first chamber are separated, the part of the second chamber exposed outside the first chamber can gradually withdraw from the space where the first chamber is located.

[0012] When the first chamber and the second chamber are in a combined state, only one of the chambers can be sealed because they have a common opening. When the first chamber and the second chamber are separated, the second chamber needs to be independently sealed. Therefore, a sealing device specifically for the second chamber is required. When the first chamber and the second chamber are separated, the first chamber will inevitably generate a new opening that may leak liquid in addition to the original opening due to the withdrawal of the second chamber. At this time, this opening also needs to be sealed to prevent the liquid sample from flowing out. Therefore, a sealing device for the separation part is required.

[0013] In some preferred ways, the second chamber collects samples through the first chamber. The second chamber can be detachably connected or combined with the first chamber. The second chamber is combined with the first chamber during the sample collection process, and the second chamber can be pushed out of the first chamber after the sample collection is completed.

[0014] In some preferred ways, the second chamber includes a second collection port for collecting samples. When the second chamber collects samples, the second collection port can be in fluid communication with the liquid inside the first chamber. In some preferred ways, the second chamber is disposed at the bottom of the first chamber. In some preferred ways, the first chamber has a first collection port for collecting samples. In some preferred ways, the opening directions of the first collection port and the second collection port are the same.

[0015] In some preferred embodiments, while loading the liquid sample into the first chamber, the liquid sample can enter the second chamber naturally. In some preferred embodiments, while loading the liquid sample into the first chamber, the liquid sample can enter the second chamber under an external force.

[0016] In some preferred embodiments, a channel for assembling the second chamber is provided at the bottom of the first chamber. In some preferred embodiments, the second chamber can move within the channel. In some preferred embodiments, the second chamber and the channel are detachably combined or connected. In some preferred embodiments, the first channel is in fluid communication with the liquid inside the first chamber. In some preferred embodiments, when the second chamber channel collects the sample, the second chamber is assembled within the channel. In some preferred embodiments, after the second chamber completes sample collection, it can move in a direction away from the first chamber under an external force.

[0017] In a second aspect, the present invention provides specific sealing structures, which may include a second sealing element for sealing the second chamber, and a third sealing element for sealing the separation between the first chamber and the second chamber when they are separated.

[0018] It should be noted that the first, second, and third in the present invention do not represent actual quantities. For example, the present invention may only include the second and / or the third, not necessarily including the first, or it may only include the first and / or the third, not necessarily including the second, or it may only include the first and / or the second, not necessarily including the third. The reason for adding the qualifiers of the first, second, or third is only for the convenience of expression and the correspondence of the relationships between the components. Here, one, two, and three do not represent numbering or sorting according to actual quantities.

[0019] In some preferred embodiments, the first sealing element and the opening of the first chamber are sealed by a first threaded structure, the second sealing element and the opening of the second chamber are sealed by a second threaded structure, and the third sealing element and the separation are sealed by a third threaded structure. In some preferred embodiments, these sealing structures may further include a first sealing element for sealing the first chamber.

[0020] In some preferred embodiments, the first sealing element and the second sealing element are linked. When the first sealing element seals the first chamber, the second sealing element can also seal the second chamber. It is possible that when the first sealing element completes sealing, the second sealing element also completes sealing, or before the first sealing element completes sealing, the second sealing element has already completed sealing.

[0021] In some preferred embodiments, the first sealing element and the third sealing element are interlocked. During the process of the first sealing element sealing the first cavity, the third sealing element can also seal the separation location. It is possible that when the first sealing element completes the sealing, the second sealing element also completes the sealing, or, before the first sealing element completes the sealing, the third sealing element has already completed the sealing.

[0022] In some preferred embodiments, the second sealing element and the third sealing element are interlocked. During the process of the second sealing element sealing the second cavity, the third sealing element can also seal the separation location. It is possible that when the second sealing element completes the sealing, the third sealing element also completes the sealing, or, before the third sealing element completes the sealing, the second sealing element has already completed the sealing.

[0023] Since there is a need for an interlocked relationship among the first sealing element, the second sealing element, and the third sealing element, some interlocking structures must be provided between these sealing elements.

[0024] In a third aspect, the present invention provides an interlocking structure for the sealing elements, including the sealing elements of the sample collection device, and the sealing elements are interlocked during the sealing process through interlocking elements.

[0025] In some preferred embodiments, the interlocking structure includes a first sealing element for sealing the first cavity and a second sealing element for sealing the second cavity, and the first sealing element and the second sealing element are interlocked through a first interlocking element. In some preferred embodiments, the interlocking structure includes a first sealing element for sealing the first cavity and a third sealing element for sealing the channel, and the first sealing element and the third sealing element are interlocked through a first interlocking element. In some preferred embodiments, the interlocking structure includes a second sealing element for sealing the second cavity and a third sealing element for sealing the channel, and the second sealing element and the third sealing element are interlocked through a second interlocking element. In some preferred embodiments, it includes a first sealing element for sealing the first cavity, a second sealing element for sealing the second cavity, and a third sealing element for sealing the channel, the first sealing element and the second sealing element are interlocked through a first interlocking element, and the second sealing element and the third sealing element are interlocked through a second interlocking element. In some preferred embodiments, it includes a first sealing element for sealing the first cavity, a second sealing element for sealing the second cavity, and a third sealing element for sealing the channel, the first sealing element and the second sealing element are interlocked through a first interlocking element, and the second sealing element and the third sealing element are interlocked through a tight fit or fixed connection.

[0026] In some preferred embodiments, the linkage between the second sealing element and the third sealing element can be achieved by a fixed connection. In some preferred embodiments, the linkage between the first sealing element and the third sealing element can be achieved by a fixed connection.

[0027] In some preferred embodiments, the first linkage element is a linkage rod. In some preferred embodiments, the second linkage element is a linkage pin. In some preferred embodiments, the term "linkage" refers to synchronous rotation. In some preferred embodiments, the term "linkage" refers to synchronous movement in a direction closer to the interior of the sample.

[0028] In some preferred embodiments, the first linkage element is a linkage rod. The two ends of the linkage rod are respectively connected to the first sealing element and the third sealing element. When one of the first sealing element and the third sealing element rotates, it can drive the other to rotate accordingly. This rotation can be synchronous or intermittent, but usually in the same direction. In some preferred embodiments, a shaft hole is provided on the inner cover surface of the first sealing element, and the linkage rod is inserted into the shaft hole. In some preferred embodiments, a shaft hole is provided on the outer cover surface of the third sealing element, and the linkage rod is inserted into the shaft hole. In some preferred embodiments, the linkage rod passes through the first chamber to connect the first sealing element and the third sealing element.

[0029] In some preferred embodiments, the linkage rod has a certain shape. Generally speaking, this shape is not a perfect circle and can be, for example, square, semi-circular or triangular, etc. Correspondingly, the shaft holes on the first sealing element and the third sealing element are also of the same shape. Then, the circumferential limit between the linkage rod and the shaft holes is achieved through this shape, and relative rotation will not occur. Therefore, the linkage rod can drive the first sealing element and the third sealing element to rotate through the two shaft holes, thereby realizing the linkage. In some other possible embodiments, the linkage rod and the shaft holes can also be tightly fitted round holes. In this case, the force of the linkage comes from the frictional force between the contacting surfaces of the linkage rod and the shaft holes. In some other possible embodiments, the linkage rod and the shaft holes can also have a step-by-step linkage cooperation relationship. Under this step-by-step linkage cooperation relationship, the linkage rod or the shaft holes can rotate independently by a certain angle and then rotate synchronously. While rotating, the first sealing element can seal the first chamber, and during the process of the first sealing element sealing the first chamber, the third sealing element can seal the separation part.

[0030] As another implementation form of the linkage cooperation, a shaft hole is provided on the linkage rod, and a cooperation element linked to the shaft hole is provided on the first sealing element or the third sealing element. The cooperation element has the same shape as the shaft hole, and this shape is offset from the axis of the linkage rod or is non-circular, so that the linkage rod and the cooperation element can achieve linkage. In some preferred embodiments, the linkage rod can also have a step-by-step linkage cooperation with the cooperation element.

[0031] In some preferred embodiments, the linkage structure may further include a second linkage element for linking the second sealing element and the third sealing element. The second linkage element is a linkage pin or a short object with a similar shape. The linkage pin can be fixedly connected to one of the second sealing element or the third sealing element, and a linkage hole matching the linkage pin is provided on the other one. Similarly, the linkage pin and the linkage hole also have a certain shape, which is similar to that of the linkage rod, and can deviate from the rotation center of the second sealing element or the third sealing element, or is non-circular. This shape can be, for example, square, semi-circular or triangular, etc., so that one of the linkage pin and / or the linkage hole can drive the other one to rotate accordingly. This rotation can be synchronous or intermittent, and this rotation can be in the same direction or in the opposite direction. While rotating, the third sealing element can seal the separation part. During the process of the third sealing element sealing the separation part, the second sealing element can seal the second chamber.

[0032] As a specific implementation form of the linkage cooperation, a linkage hole is provided on the inner cover surface of the third sealing element, and the linkage pin is inserted into the linkage hole, and the other end is fixedly connected to the outer cover surface of the second sealing element. Or, a linkage hole is provided on the outer cover surface of the second sealing element, and one end of the linkage pin is inserted into the linkage hole, and the other end is fixedly connected to the inner cover surface of the third sealing element. In this implementation manner, the linkage between the second sealing element and the first sealing element is transmitted through the third sealing element. That is to say, during the process of the first sealing element sealing the first chamber, the third sealing element can seal the separation part, and at the same time, the second sealing element can seal the second chamber.

[0033] In some preferred embodiments, the second sealing element and the third sealing element may not be provided with a second linkage element, but realize linkage through their own structural cooperation. For example, a certain surface of the second sealing element and the third sealing element is fixedly connected together, so that the two sealing elements can rotate synchronously.

[0034] Fourthly, the present invention provides a specific form of the separation part. In some preferred embodiments, the separation part may be in the form of a channel, for example, a sealable channel in which the second chamber can be inserted and installed. In some preferred embodiments, the channel is located at the bottom of the first chamber (that is, the channel and the opening of the first chamber for collecting liquid samples are respectively located at both ends of the first chamber). In this way, when collecting a liquid sample, usually the opening is facing upwards and the channel is facing downwards, which facilitates the liquid sample to directly enter the second chamber due to the natural action of gravity while entering the first chamber. In some preferred embodiments, a slope slightly inclined towards the channel may be provided at the bottom of the first chamber, and the liquid sample will flow along this slope towards the channel. The slightly inclined angle can be within 5°.

[0035] In some preferred modes, before the product is used, the second cavity is installed in the channel. Of course, this installation method must be detachable, for example, the second cavity is inserted in the channel, and its outer wall is tightly matched with the inner wall of the channel. In some preferred modes, the tight fit between the second cavity and the channel must be sealed, and this seal can be achieved by assembling the material itself, or by adding a sealing component on the mating surface.

[0036] In some preferred embodiments, the channel has an opening connected to the first cavity, and the second cavity also has an opening for collecting samples. After the second cavity is assembled in the channel, the opening on the second cavity must be consistent with the opening of the channel, so that the liquid sample can be collected in the second cavity. In some preferred embodiments, the opening on the second cavity is flush with the surface of the opening of the channel. In other preferred embodiments, the surface of the opening of the second cavity is slightly higher than the surface of the opening of the channel. In other preferred embodiments, the opening of the second cavity and the opening surface of the channel form a smooth transition and a downwardly inclined bottom from the inside to the outside. Regarding the design and coordination of the structure at the opening, the main function is to avoid the accumulation of liquid samples in the gap between the second cavity and the channel as much as possible, because such accumulation can easily cause the second cavity and the channel to leak during the separation process. Through the high and low stratification of the structure, the liquid sample can flow along this structure to other positions at the bottom of the first cavity, rather than accumulating at the coordination of the second cavity and the channel. In addition to the sealing between the mating surfaces, the structural design at the opening can also be used to strengthen the seal to avoid any leakage as much as possible.

[0037] In some preferred embodiments, the cooperation between the second cavity and the channel needs to be limited, otherwise the second cavity may slide into the first cavity through the channel. This situation must be avoided because not only will the exposed part of the second cavity enter the first cavity and cause contamination to the liquid sample, but also the channel itself is an opening for the first cavity. Originally, the second cavity can be used as a plug for the channel. If the second cavity falls into the first cavity, it will inevitably cause a hole in the channel and a large amount of liquid sample will leak. In some preferred embodiments, the inner wall of the channel and the outer wall of the second cavity are provided with a mutually matching limiting structure. This limiting structure limits the extreme position of the second cavity installation to a certain place in the channel. Once the second cavity is installed in place, it will not move inward any further.

[0038] As a specific implementation of the limiting structure, an outer step surface is provided on the inner wall of the channel. Taking the outer step surface as the demarcation line, the inner diameter of the part close to the first cavity is larger than that of the part close to the outside. At the same time, an inner step surface that cooperates with this outer step surface is provided on the outer wall of the second cavity. Taking the inner step surface as the demarcation line, the inner diameter of the part close to the first cavity is smaller than that of the part close to the outside. Then, the cooperation between the inner step surface and the outer step surface can limit the extreme installation position of the second cavity at this cooperation point. When the second cavity is installed, due to the step surface blocking, it cannot move further inward, realizing the limitation of the second cavity.

[0039] The channel is a structure that enables the first cavity and the second cavity to be assembled. The channel can be regarded as a part of the first cavity. In some preferred ways, after the second cavity is installed, the outer contour of the second cavity does not exceed the outer surface of the channel. This situation can make the first cavity and the second cavity form a whole before use. However, in this case, it is impossible to take out the second cavity. Therefore, in some preferred ways, when the sealing element seals the first cavity, the second cavity and the separation part, it can gradually push the second cavity outwards. When all the sealing processes (including the sealing of the first cavity by the first sealing element, the sealing of the second cavity by the second sealing element, and the sealing of the separation part by the third sealing element) are completed, a part of the second cavity extends beyond the outer contour of the first cavity. At this time, the second cavity can be separated from the first cavity through this extended part. The second cavity at the separation part is a cavity that has been sealed, and the liquid sample inside it can be used for secondary confirmation testing. After the second cavity is separated from the first cavity, it can be independently transported to the secondary testing institution.

[0040] In a fifth aspect, the present invention provides a test element. The test element is arranged in the first cavity and can have a relatively independent space in the first cavity. For example, a detection area that can be isolated or communicated with the first cavity is provided on the side wall of the first cavity, and the test element is placed in this detection area. The liquid sample first enters the first cavity, and then enters the detection area from the first cavity to react with the test element, thereby performing detection.

[0041] The reason for separating the detection area from the main cavity of the first cavity is to separate part of the sample from the test element, so as to ensure that at least a part of the liquid sample has not come into contact with the test element. This part will not be contaminated by the test element and can be collected into the second cavity for secondary detection and confirmation.

[0042] In some preferred ways, the position where the test element is located is visible.

[0043] Sixth aspect, the present invention provides a barrier element for blocking or opening the communication relationship between the first chamber and the test area. In the initial state of collecting the sample, the barrier element separates the first chamber from the test area. At this time, the liquid sample cannot enter the test area, and the test element will not come into contact with or react with the liquid sample. When a certain amount is collected or at a certain time, the barrier element opens the entrance connecting the first chamber to the test area, allowing a part of the liquid sample to enter the test area from the entrance and perform a preliminary inspection through the test element to obtain a preliminary test result.

[0044] In some preferred embodiments, an entrance connecting to the detection area is provided at the bottom of the first chamber, and the barrier element is disposed at the entrance. In some preferred embodiments, the barrier element and the second sealing element are linked. When the second sealing element completes the sealing of the second chamber, the barrier element is triggered to open the entrance connecting to the detection area. At this time, the sealing of the second chamber has been completed, ensuring that the liquid sample in the second chamber will not come into contact with the sample in the detection area, and guaranteeing the accuracy of the secondary detection.

[0045] In some preferred embodiments, the barrier element and the third sealing element are linked. When the third sealing element completes the sealing of the separation part, the barrier element is triggered to open the entrance connecting to the detection area. Since the second sealing element has completed the sealing of the second chamber during the process of the third sealing element sealing the separation part, when the third sealing element completes the sealing of the separation part, the sealing of the second chamber has been completed beforehand. Therefore, when the entrance to the detection area is opened at this time, the liquid sample in the second chamber will not come into contact with the sample in the detection area, and the accuracy of the secondary detection can be guaranteed.

[0046] Seventh aspect, the present invention provides a method for collecting a test sample. This method uses the sample collection device as described above. The device includes a first chamber for collecting a liquid sample, a second chamber for collecting and confirming the test liquid sample, and the first chamber and the second chamber can be combined or separated; the sample detection device further includes a second sealing element for sealing the second chamber, and a third sealing element for sealing the separation part between the first chamber and the second chamber when they are separated.

[0047] In some preferred embodiments, it further includes a first sealing element for sealing the first chamber.

[0048] In some preferred embodiments, during the process of the first sealing element sealing the first chamber, the second chamber can be sealed by the second sealing element.

[0049] In some preferred embodiments, during the process of the first sealing element sealing the first chamber, the separation part can be sealed by the third sealing element.

[0050] In some preferred embodiments, either the second sealing element or the third sealing element is linked to the first sealing element and the second sealing element is linked to the third sealing element; or both the second sealing element and the third sealing element are linked to the first sealing element.

[0051] In some preferred embodiments, the sample collection method further includes a first linkage element for linking the first sealing element and the third sealing element.

[0052] In some preferred embodiments, the sample collection method further includes a second linkage element for linking the second sealing element and the third sealing element.

[0053] In some preferred embodiments, the separation location includes a channel that is sealed by the second chamber before sample collection.

[0054] In some preferred embodiments, the second chamber is movable within the channel, and a limiting structure is provided within the channel to prevent the second chamber from sliding into the first chamber.

[0055] In some preferred embodiments, during the sealing process, the second chamber and the first chamber can move relative to each other such that a part of the second chamber is pushed out of the first chamber.

[0056] In some preferred embodiments, a blocking element is provided within the first chamber that can prevent a liquid sample from flowing into the detection area. The blocking element can open or close the entrance through which the first chamber communicates with the detection area, thereby preventing or releasing the liquid sample from flowing into the detection area; while the third sealing element seals the separation location, it triggers the blocking element to open the entrance to the detection area.

[0057] In some preferred embodiments, the method includes allowing the first chamber and the second chamber to be in liquid communication through the channel, and the liquid sample can flow from the first chamber into the second chamber through the channel or an orifice of the channel, so that while loading the liquid sample into the first chamber, the liquid sample can also naturally enter the second chamber. In some preferred embodiments, before separation of the first chamber and the second chamber, the channel is sealed. In some preferred embodiments, during the separation of the first chamber and the second chamber, the channel is sealed.

[0058] In some preferred embodiments, the method includes the process of sealing the first chamber and the second chamber that have collected the sample. During the sealing process, the first chamber and the second chamber can move relative to each other such that a part of the second chamber is pushed out of the first chamber. In some preferred embodiments, during the sealing process, a part of the second chamber is exposed outside the first chamber. In some preferred embodiments, the sealing of the first chamber is accompanied by the sealing of the second chamber.

[0059] In some preferred embodiments, the method includes a process of sealing the channel connecting the first chamber and the second chamber. During the sealing process, the first chamber and the second chamber can move relative to each other, so that a part of the second chamber is pushed out of the first chamber. In some preferred embodiments, during the sealing process, a part of the second chamber is exposed outside the first chamber. In some preferred embodiments, the sealing process of the first chamber is accompanied by the sealing of the channel. In some preferred embodiments, the sealing process of the channel is after the sealing process of the second chamber. In some preferred embodiments, while sealing the channel, the second chamber is pushed outwards.

[0060] In an eighth aspect, the present invention provides a sample detection method, which is used to detect whether there is an analyte in the collected liquid sample. The detection method includes using the above-mentioned collection device or collection method to collect the sample to be detected. After a certain amount of sample is collected in the first chamber, the sample in the first chamber is initially detected.

[0061] In some preferred embodiments, first, the second chamber that has completed sample collection is sealed, and then the sample in the first chamber is detected by a test element.

[0062] In some preferred embodiments, first, the first chamber and the second chamber are separated, and then the sample in the first chamber is detected by a test element. Since the liquid sample collected in the second chamber is for secondary detection, separating the second chamber first can prevent the liquid sample in the second chamber for secondary detection from being contaminated by the test element in the initial detection.

[0063] In some preferred embodiments, first, the channel connecting the first chamber and the second chamber is sealed, and then the sample in the first chamber is detected by a test element.

[0064] In some preferred embodiments, during the process of collecting the sample, the detection area and the sample collection area are isolated by a barrier element. After the sample collection is completed, the isolation between the test area and the sample collection area by the barrier element is removed, so that the sample can enter the detection area.

[0065] In some preferred embodiments, a detection area is arranged in the first chamber. In some preferred embodiments, a test element is arranged in the detection area. In some preferred embodiments, an inlet that can be connected or closed is provided between the detection area and the first chamber. In some preferred embodiments, the inlet can cooperate with the barrier element, be blocked by the barrier element and closed, or be opened to connect the detection area and the first chamber.

[0066] The following embodiments should also be included in the technical solution of the present invention:

[0067] A first chamber for collecting a liquid sample, a second chamber for collecting a confirmation test liquid sample, the first chamber and the second chamber being capable of being combined or separated; the sample detection device further includes a second sealing element for sealing the second chamber, and a third sealing element for sealing the separation between the first chamber and the second chamber.

[0068] In some preferred embodiments, when the first chamber and the second chamber are combined, the first chamber and the second chamber can be in a liquid communication state.

[0069] In some preferred embodiments, after the second sealing element seals the second chamber, the third sealing element seals the separation.

[0070] In some preferred embodiments, after the second sealing element completes the sealing of the second chamber, the third sealing element completes the sealing of the separation.

[0071] In some preferred embodiments, it further includes a first sealing element for sealing the first chamber.

[0072] In some preferred embodiments, during the process of the first sealing element sealing the first chamber, the second chamber can be sealed by the second sealing element.

[0073] In some preferred embodiments, during the process of the first sealing element sealing the first chamber, the separation can be sealed by the third sealing element.

[0074] In some preferred embodiments, one of the second sealing element and the third sealing element is linked with the first sealing element and the second sealing element is linked with the third sealing element.

[0075] In some preferred embodiments, both the second sealing element and the third sealing element are linked with the first sealing element. In some preferred embodiments, the sample detection device further includes a first linkage element for linking the first sealing element and the third sealing element.

[0076] In some preferred embodiments, the linkage refers to synchronous rotation.

[0077] In some preferred embodiments, the linkage refers to synchronous movement in a direction approaching the interior of the sample.

[0078] In some preferred embodiments, the separation includes a channel, and the second chamber can move within the channel.

[0079] In some preferred embodiments, a limiting structure for the second chamber is provided within the channel.

[0080] In some preferred embodiments, after the second chamber is sealed, it can move along the channel in a direction away from the first chamber.

[0081] In some preferred embodiments, a detection area for initially collecting a sample is included, and the detection area can be opened or closed.

[0082] In some preferred embodiments, when the second chamber has not collected a sample or is collecting a sample, the detection area is closed, and when the collection in the second chamber is completed and sealed, the sample can enter the detection area.

[0083] In some preferred embodiments, a barrier element is further included, and the barrier element can open or close a detection entrance.

[0084] In some preferred embodiments, the barrier element can be linked with a third sealing element and open the detection area entrance under the drive of the third sealing element.

[0085] In some preferred embodiments, the barrier element can be linked with a second sealing element and open the detection area entrance under the drive of the second sealing element.

[0086] In some preferred embodiments, the barrier element can be linked with a first sealing element and open the detection area entrance under the drive of the first sealing element.

[0087] A method for collecting a sample, which first allows the sample to enter a chamber, stores the sample for initial detection and the sample for secondary confirmation detection in separate chambers, and after the sample collection is completed, first isolates the sample for secondary confirmation detection alone, and then detects the sample for initial detection.

[0088] A method for collecting a sample, which provides a sample collection device including a first chamber for collecting a liquid sample and a second chamber for collecting a confirmation detection liquid sample, and the first chamber and the second chamber can be combined or separated; the sample detection device further includes a second sealing element for sealing the second chamber and a third sealing element for sealing the separation between the first chamber and the second chamber.

[0089] In some preferred embodiments, first load the sample into the first chamber, and then load the sample into the second chamber.

[0090] In some preferred embodiments, the first chamber and the second chamber are loaded with samples simultaneously.

[0091] In some preferred embodiments, when the first chamber and the second chamber are combined, the first chamber and the second chamber can be in a liquid communication state.

[0092] In some preferred embodiments, after the second chamber completes sample collection, use the second sealing element to seal the second chamber.

[0093] In some preferred embodiments, after sealing the second chamber, use the third sealing element to seal the separation.

[0094] In some preferred solutions, while sealing the second cavity, a third sealing element is used to seal the separation point.

[0095] In some preferred embodiments, a first sealing element for sealing the first cavity is also included.

[0096] In some preferred solutions, after the first cavity and the second cavity complete sample collection, the first cavity is sealed with a first sealing element.

[0097] In some preferred solutions, the first sealing element is used to seal the first cavity, while the second sealing element is used to seal the second cavity.

[0098] In some preferred solutions, the first sealing element is used to seal the first cavity, and the third sealing element is used to seal the separation point at the same time.

[0099] In some preferred solutions, the second sealing element and the third sealing element seal in conjunction with each other.

[0100] In some preferred solutions, the first sealing element and the second sealing element seal in a linked manner.

[0101] In some preferred solutions, the first sealing element and the third sealing element seal in conjunction with each other.

[0102] In some preferred embodiments, the linkage refers to synchronous rotation.

[0103] In some preferred embodiments, the linkage refers to synchronous movement toward the interior of the sample.

[0104] In some preferred embodiments, the separation includes a channel, and the second cavity is movable in the channel.

[0105] In some preferred solutions, after the second cavity is sealed, its passage moves in a direction to separate from the first cavity. A method for detecting a sample uses the above method to collect the sample and includes a detection area, which can be opened or closed.

[0106] In some preferred embodiments, the second cavity is not collecting samples or is closing the detection area when collecting samples. When the collection of the second cavity is completed and sealed, the detection area is opened to allow the sample to enter and be detected.

[0107] In some preferred solutions, a barrier element is further included, and the barrier element can open or close the detection entrance.

[0108] In some preferred solutions, the blocking element can be linked with the third sealing element and open the entrance of the detection area driven by the third sealing element.

[0109] In some preferred embodiments, the barrier element can be linked to the second sealing element and open the detection area entrance under the drive of the second sealing element.

[0110] In some preferred embodiments, the barrier element can be linked to the first sealing element and open the detection area entrance under the drive of the first sealing element.

[0111] The beneficial effects of the present invention are as follows: With the above structure, it has the characteristics of simple and reasonable structure, low material cost, excellent performance; and it is convenient for secondary detection. In particular, when subsequent confirmation detection is required, there is no need to send the entire detection device to a testing institution for detection. Instead, only the second chamber is removed from the device and then sent to the detection structure. This is not only safe, but also saves space, cost, and is more environmentally friendly. BRIEF DESCRIPTION OF THE DRAWINGS

[0112] Figure 1 is the overall structural external view of the present invention.

[0113] Figure 2 is the overall structural cross-sectional view after the present invention is sealed.

[0114] Figure 3 is the overall structural exploded view of the present invention.

[0115] Figure 4 is the overall structural cross-sectional exploded view of the present invention.

[0116] Figure 5 is the overall structural external view after the second chamber is sealed.

[0117] Figure 6 is the overall structural cross-sectional view after the second chamber is sealed.

[0118] Figure 7 is the overall structural cross-sectional view before sealing.

[0119] Figure 8 is the overall structural cross-sectional view of the first stage of sealing.

[0120] Figure 9 is the three-dimensional view of the first chamber.

[0121] Figure 10 is the cross-sectional view of the first chamber.

[0122] Figure 11 is the structural diagram of the first sealing element.

[0123] Figure 12 is the schematic diagram of the first linkage element.

[0124] Figure 13 is the cross-sectional view of the present invention with the second chamber hidden, showing the position of the sealing ring.

[0125] Figure 14 It is another implementation manner of the sealing element.

[0126] Reference numerals in the figure: first cavity 1, second cavity 2, detection area 3, sample inlet 4, channel 5, first collection port 6, second collection port 7, outer stepped surface 8, inner stepped surface 9, first sealing element 10, second sealing element 11, first sealing part 12, second sealing part 13, shoulder 14, opening 15 of channel 5, third sealing element 16, first thread structure 17, second thread structure 18, third thread structure 19, first linkage element 20, second linkage element 21, inner cover surface 22, inner shaft hole 23, outer cover surface 24, linkage hole 25, coupling pin 26, upper cover surface 27 of the second sealing element, lower cover surface 28 of the third sealing element, inner stepped surface 29, gap 30, barrier element 31, inlet 32 of the detection area 3, retaining piece 33, protrusion 34, limiting ring 35, outer stepped surface 36, sealing ring 37. Detailed implementation manners

[0127] The following further elaborates on the present invention with reference to the accompanying drawings. It should be noted that the embodiments are only specific descriptions of the present invention and should not be regarded as limitations of the present invention.

[0128] First, further explanations are made on the structures or technical terms involved in the present invention. If not specifically specified, they are understood and interpreted according to the general technical terms commonly used in the art.

[0129] Detection

[0130] Detection means assaying or testing for the presence of a substance or material, such as, but not limited to, chemical substances, organic compounds, inorganic compounds, metabolites, drugs or drug metabolites, organic tissues or metabolites of organic tissues, nucleic acids, proteins or polymers. Additionally, detection means testing the quantity of a substance or material. Further, assay also means immunoassay, chemical assay, enzyme assay, etc.

[0131] Sample

[0132] The sample that can be detected by the detection device of the present invention includes biological liquids (such as case liquids or clinical samples). The liquid sample or liquid samples can be derived from solid or semi-solid samples, including excreta, biological tissues, and food samples. Any suitable method can be used to convert the solid or semi-solid sample into a liquid sample, such as mixing, mashing, macerating, incubating, dissolving, or digesting the solid sample by enzymatic action in a suitable solution (such as water, phosphate solution, or other buffer solutions). "Biological samples" include those derived from animals, plants, and food samples, such as urine, saliva, blood and its components, cerebrospinal fluid, vaginal secretions, sperm, feces, sweat, secretions, tissues, organs, tumors, cultures of tissues and organs, cell cultures, and media derived from humans or animals. Preferably, the biological sample is urine. Food samples include food processing substances, end products, meat, cheese, wine, milk, and drinking water. Plant samples include those derived from any plant, plant tissue, plant cell culture, and media. "Environmental samples" are derived from the environment (e.g., liquid samples from lakes or other water bodies, sewage samples, soil samples, groundwater, seawater, and waste liquid samples). Environmental samples may also include sewage or other wastewater.

[0133] Using the present invention and a suitable detection element, any analyte can be detected. Preferably, the present invention is used to detect small drug molecules in saliva and urine. Of course, the collection device of the present invention can collect any of the above forms of samples, whether initially solid or liquid. As long as these liquid or liquid samples flow into the first chamber, these liquid samples can flow into the second chamber simultaneously or later. Since the second chamber can be combined with or separated from the first chamber, when initially collecting the sample, the first chamber and the second chamber are combined, and the user can complete the collection of the liquid samples in the first chamber and the second chamber through a single collection action. When subsequent confirmation testing is required, the second chamber is separated from the first chamber. Thus, the liquid in the first chamber can be subjected to primary testing, while the second chamber can be subjected to secondary testing. Optionally, the functional positions of the first chamber and the second chamber can also be swapped, that is, the liquid in the second chamber can be subjected to primary testing, while the liquid in the first chamber can be subjected to secondary testing.

[0134] Test component

[0135] The test element can be a lateral flow test strip, which can detect a variety of analytes. Of course, other suitable test elements can also be used in the present invention. Any element that can detect whether a sample or specimen contains the analyte of interest can be called a test element, regardless of the technical principle underlying this detection, such as immunology, chemistry, electrochemistry, optics, physics, etc.

[0136] A variety of test elements can be combined and applied to the present invention. One form is a test strip. The test strip for analyzing analytes (such as drugs or metabolites indicating physical conditions) in a sample can be in various forms, such as immunoassay or chemical analysis forms. The test strip can adopt non-competitive or competitive analysis modes. The test strip includes a water-absorbing material with a sample application area, a reagent area, and a test area. Add the sample to the sample application area, and it flows to the reagent area through capillary action. In the reagent area, if the analyte is present, the sample binds to the reagent. Then the sample continues to flow to the detection area. Some other reagents, such as molecules specifically binding to the analyte, are immobilized in the detection area. These reagents react with the analyte (if present) in the sample and bind the analyte in this area, or bind to one of the reagents in the reagent area. The marker for displaying the detection signal is present in the reagent area or a separate marker area.

[0137] A typical non-competitive analysis mode is that if the analyte is contained in the sample, a signal will be generated, and if it is not contained, no signal will be generated. In the competitive method, if the analyte is not present in the sample, a signal is generated, and if the analyte is present, no signal is generated.

[0138] The test element is a test strip, and materials that can absorb water or not can be selected. The test strip can include various materials for liquid sample transfer. One material of the test strip can cover another material, such as filter paper covering a nitrocellulose membrane. One or more materials can be selected for one area of the test strip, while other different one or more materials can be selected for another area. The test strip can be adhered to a certain support or a hard surface to improve the strength of holding the test strip.

[0139] The analyte is detected through a signal generation system. For example, using one or more enzymes that specifically react with this analyte, and by using the method of immobilizing specific binding substances on the test strip as described above, a composition of one or more signal generation systems is immobilized in the analyte detection area of the test strip. The substance generating the signal can be in the sample application area, the reagent area, or the detection area, or throughout the test strip, and this substance can fill one or more materials of the test strip. Add a solution containing the signal substance to the surface of the test strip or immerse one or more materials of the test strip in the solution containing the signal substance. Dry the test strip added with the solution containing the signal substance.

[0140] The various zones of the test strip can be arranged in the following manner: a sample application zone, a reagent zone, a detection zone, a control zone, a zone for determining whether the sample is adulterated, and a liquid sample absorption zone. The control zone is located after the detection zone. All the zones can be arranged on a single test strip made of only one material, or different zones can use different materials. Each zone can be in direct contact with the liquid sample, or the different zones can be arranged according to the flow direction of the liquid sample, with the ends of each zone connected to and overlapping the front end of another zone. The materials used can be materials with good water absorption properties such as filter paper, glass fiber, or nitrocellulose membrane, etc. The test strip can also be in other forms.

[0141] The commonly used reagent strip is usually a nitrocellulose membrane reagent strip, that is, the detection area includes a nitrocellulose membrane, and specific binding molecules are immobilized on the nitrocellulose membrane to show the detection result; it can also be an acetate cellulose membrane or a nylon membrane, etc. For example, some reagent strips or devices containing reagent strips described in the following patents: US 4857453; US 5073484; US5119831; US 5185127; US 5275785; US 5416000; US 5504013; US 5602040; US 5622871; US5654162; US 5656503; US 5686315; US 5766961; US 5770460; US 5916815; US 5976895; US6248598; US 6140136; US 6187269; US 6187598; US 6228660; US 6235241; US 6306642; US6352862; US 6372515; US 6379620; and US 6403383. The test strips and similar devices with test strips disclosed in the above patent documents can all be applied to the test element or detection device of the present invention for detecting the analyte, such as detecting the analyte in a sample.

[0142] The detection reagent strip applied to the present invention can be the commonly referred to lateral flow test strip. The specific structures and detection principles of these detection reagent strips are well-known to those of ordinary skill in the art in the prior art. Ordinary detection reagent strips include a sample collection area, a labeling area, a detection area, and a water absorption area. The sample collection area includes a sample receiving pad, the labeling area includes a labeling pad, and the water absorption area may include a water absorption pad. The detection area includes the necessary chemical substances for detecting whether the analyte is contained, such as immunoassay reagents or enzyme chemical reagents. Commonly used detection reagent strips are nitrocellulose membrane reagent strips, that is, the detection area includes a nitrocellulose membrane, and specific binding molecules are fixed on the nitrocellulose membrane to display the detection results; it can also be a cellulose acetate membrane or a nylon membrane, etc. Of course, a detection result control area may also be included downstream of the detection area. Usually, the control area and the detection area appear in the form of horizontal lines, which are detection lines or control lines. Such detection reagent strips are traditional reagent strips. Of course, it can also be other types of reagent strips that utilize capillary action for detection. In addition, generally, the detection reagent strip has dry chemical reagent components, such as immobilized antibodies or other reagents. When exposed to liquid, the liquid flows along the reagent strip by capillary action. As it flows, the dry reagent components dissolve in the liquid, and then react with the dry reagents in the next area to perform the necessary detection. The liquid flow is mainly carried out by capillary action. The descriptions and records of these test elements can be found in the following documents: "Research on the Regeneration Treatment of Nitrocellulose Membrane and Its Protein Adsorption Capacity" by Li Fugang; "Analysis of the Performance of Chromatographic Membrane Materials in Colloidal Gold Diagnostic Kits" by Ma Hongyan, Li Qiang, etc.; "A New Type of Colloidal Gold Immunochromatographic Test Strip" by Wang Yong, Wang Luhai, etc.

[0143] Here, they can all be applied to the detection device of the present invention, or be arranged in the detection chamber to contact the liquid sample, or be used to detect the presence or quantity of the analyte in the liquid sample entering the detection chamber.

[0144] In addition to the above test element in the form of a reagent strip, which is itself used to contact the liquid in the first chamber 1 to test whether the liquid sample contains the analyte, in some preferred embodiments, the test element can also be disposed on a centralized test card. The test card has a plurality of grooves, and the test element is located in the grooves. The entire test card is disposed in the detection area 3. Since there is an inlet 4 connecting the first chamber 1 and the detection area 3, the liquid sample entering the first chamber 1 can enter the detection area 3 through the inlet 4 and thus be detected by the test element on the test card. Of course, in addition to the above disclosed carriers, other carriers can also be used in the present invention as carriers for carrying the test strip. For example, in some embodiments, the first chamber can first collect the liquid sample, and then a separate test strip or a card or carrier with a test strip is inserted into the first chamber for detection. Those of ordinary skill in the art can understand that according to the description of the present invention, these test strips can be independent without being disposed on a carrier, and the detection area 3 of the present invention can also be absent in some cases, and the test strip can also be absent in some cases.

[0145] Analyte

[0146] Examples of analytes involved in the present invention include some small molecule substances, which include drugs (such as abused drugs). "Drug of abuse" (DOA) refers to the non-medical use of drugs (usually acting as nerve paralytics). The abuse of these drugs can cause physical and mental damage, dependence, addiction and / or death. Examples of drug abuse include cocaine; amphetamine AMP (e.g., black beauties, white amphetamine tablets, dextroamphetamine, dextroamphetamine tablets, Beans); methamphetamine MET (crank, meth, crystal, speed); barbiturates BAR (such as Valium, Roche Pharmaceuticals, Nutley, New Jersey); sedatives (i.e., sleep aids); lysergic acid diethylamide (LSD); depressants (downers, goofballs, barbs, blue devils, yellow jackets, methaqualone); tricyclic antidepressants (TCA, i.e., imipramine, amitriptyline, and doxepin); 3,4-methylenedioxymethamphetamine (MDMA); phencyclidine (PCP); tetrahydrocannabinol (THC, pot, dope, hash, weed, etc.); opiates (i.e., morphine MOP or, opium, cocaine COC;, heroin, hydrocodone); anxiolytics and sedative-hypnotics, anxiolytics are a class of drugs mainly used to relieve anxiety, tension, fear, stabilize emotions, and have hypnotic and sedative effects, including benzodiazepines BZO (benzodiazepines), atypical BZ classes, fused diazepines NB23C classes, benzazepines, ligands of BZ receptors, open-ring BZ classes, diphenylmethane derivatives, piperazine carboxylates, piperidine carboxylates, quinazolinones, thiazine and thiazole derivatives, other heterocyclic classes, imidazole-type sedative / painkillers (such as hydrocodone OXY, methadone MTD); propylene glycol derivatives - carbamates, aliphatic compounds, anthracene derivatives, etc. The detection device of the present invention can also be used for the detection of drugs that are for medical use but are prone to overdose, such as tricyclic antidepressants (imipramine or analogs) and acetaminophen, etc. These drugs are metabolized into small molecule substances after being absorbed by the human body, and these small molecule substances are present in body fluids such as blood, urine, saliva, sweat, etc. or some of the body fluids contain the above small molecule substances.

[0147] For example, the analytes detected by the present invention include, but are not limited to, creatinine, bilirubin, nitrite, protein (non-specific), hormones (e.g., human chorionic gonadotropin, progesterone hormone, follicle-stimulating hormone, etc.), blood, white blood cells, sugar, heavy metals or toxins, bacterial substances (such as proteins or carbohydrates specific to certain bacteria, such as Escherichia coli 0157:H7, Staphylococcus, Salmonella, Clostridium, Campylobacter, L. monocytogenes, Vibrio, or Bacillus cereus), and substances related to physiological characteristics in urine samples, such as pH and specific gravity. Any other clinical urine chemical analysis can be performed using the lateral flow detection format in combination with the device of the present invention.

[0148] Combination and separation of the first chamber and the second chamber

[0149] The sample detection device of the present invention includes two chambers for collecting liquid samples - a first chamber 1 and a second chamber 2. The first chamber 1 is used to collect the liquid sample for the initial detection, and the second chamber 2 is used to collect the liquid sample for the secondary confirmation detection. The first chamber 1 and the second chamber 2 can be combined together so that they can simultaneously receive the initially collected liquid sample. That is to say, when the user uses the product of the present invention, only one collection operation is required to inject the liquid sample into both the first chamber 1 and the second chamber 2 at the same time.

[0150] In some embodiments of the present invention, as Figure 2 、 7 、shown in 8, a channel 5 is provided at the bottom of the first chamber 1. One end of the channel 5 communicates with the inside of the first chamber 1, and the other end communicates with the outside. The second chamber 2 can be assembled into the channel 5, and the bottom of the second chamber 2 is sealed to form a plug for the channel 5. As described above, since the sample has a certain fluidity, when the user collects the sample, the collection opening is usually facing upwards. For example Figure 9 、 10 、shown, the first collection opening 6 is facing upwards, and the position of the channel 5 (i.e., the mouth 7 of the second chamber 2) is below the first collection opening 6. When the user injects the fluid sample from the first collection opening 6, the sample will flow along the inner wall of the first chamber 1 into the second chamber 2 due to the action of gravity and thus be collected by the second chamber 2. That is to say, the second chamber 2 at the bottom will be filled with the sample first, and then the first chamber 1 will continue to be filled until the amount required for one detection is reached.

[0151] Since the liquid sample in the first chamber 1 is directly used for detection, and the liquid sample in the second chamber 2 may be sealed and transported to the confirmation testing agency for secondary confirmation testing. Therefore, since the liquid sample in the first chamber 1 has undergone an initial test and has become a non-raw sample, testing the sample in the first chamber 1 will affect the accuracy of the secondary confirmation test results. Therefore, it is necessary to separate the first chamber 1 and the second chamber 2, and it is necessary to ensure that after the separation of the first chamber 1 and the second chamber 2, they can independently form a sealed cavity to encapsulate the liquid samples therein. At the same time, it is also necessary to ensure that during the separation process of the first chamber 1 and the second chamber 2, the samples will not be sealed at the separation point, which requires requirements for the structure of the separation point and the respective sealing structures of the first chamber and the second chamber.

[0152] In some preferred embodiments, the second chamber 2 collects samples through the first chamber 1, and the second chamber 2 can be detachably connected or combined with the first chamber 1. The second chamber 2 is combined with the first chamber 1 during the sample collection process and can be pushed out of the first chamber after the sample collection is completed.

[0153] In some preferred embodiments, the second chamber 2 includes a second collection port for collecting samples. When the second chamber collects samples, the second collection port can communicate with the liquid inside the first chamber. In some preferred embodiments, the second chamber is disposed at the bottom of the first chamber. In some preferred embodiments, the first chamber 1 has a first collection port 37 for collecting samples. In some preferred embodiments, the opening directions of the first collection port 37 and the second collection port 38 are the same, so as to maximize the collection efficiency of the second chamber 2 through the structure. If the directions of the first collection port 37 and the second collection port 38 are different, when the sample enters the second chamber through the first chamber, it will not be as smooth as when the collection ports have the same direction, and it is easy to cause retention on the surface or side wall. Moreover, due to the different directions, there will inevitably be a corner, and the sample may accumulate at the corner, which is not conducive to collection. The excess sample at the accumulation point will also increase the possibility of leakage. In addition, if the directions of the collection ports are different, it may cause the linkage during sealing to be not smooth.

[0154] In some preferred embodiments, when loading the liquid sample into the first chamber, the liquid sample can naturally enter the second chamber. In some preferred embodiments, when loading the liquid sample into the first chamber, the liquid sample can enter the second chamber under the action of an external force.

[0155] In some preferred embodiments, a channel for assembling the second chamber is provided at the bottom of the first chamber. In some preferred embodiments, the second chamber can move within the channel. In some preferred embodiments, the second chamber and the channel are detachably combined or connected. In some preferred embodiments, the first channel is in fluid communication with the interior of the first chamber. In some preferred embodiments, when the second chamber channel collects a sample, the second chamber is assembled within the channel. In some preferred embodiments, after the second chamber completes sample collection, it can move in a direction away from the first chamber under an external force.

[0156] Separation point

[0157] For this reason, the present invention also provides a separation location between the first chamber 1 and the second chamber 2, as Figure 7 shown. This separation location is embodied in the form of a channel 5. When the first chamber 1 and the second chamber 2 are combined, this separation location also appears as a joint between the first chamber 1 and the second chamber 2. In the initial stage, when the sample collection device has not been used, the second chamber 2 is assembled on the first chamber 1. At this time, as described above, the second chamber 2 serves as a plug at the channel 5 to prevent the sample from leaking out from the channel 5 during the initial loading. Moreover, after the sample loading is completed, the second chamber 2 must be able to withdraw from the channel 5 without carrying out excess samples. This poses relatively high requirements for the assembly and sealing between the second chamber 2 and the channel 5, such that the liquid sample preferably flows into the interior of the second chamber 2 and the interior of the first chamber 1 outside the channel 5, rather than accumulating at the assembly location between the second chamber 2 and the channel 5.

[0158] For this reason, the following several forms are designed:

[0159] First, the way that can be adopted is that the surface of the mouth 7 of the second chamber 2 (i.e., this opening of the second chamber 2 facing into the first chamber 1) and the surface of the opening 15 of the channel 5 facing into the first chamber must be at least flush. The flush surface is relatively less likely to form an accumulation point. In particular, what needs to be avoided is that the surface of the opening of the channel 5 is lower than the surface of the mouth of the second chamber 2. In this case, inevitably, the liquid sample will enter the gap between the channel 5 and the mouth of the second chamber 2, and thus will also flow out when the second chamber 2 withdraws. In addition, it should be noted that since the second chamber 2 needs to have a second sealing portion 13 with a second sealing element 11, this second sealing portion 13 may extend beyond its mouth 7, and a shoulder 14 is formed between the second sealing portion 13 and the mouth 7, as Figure 3 and 6 shown. At this time, what we need to ensure is that the shoulder 14 is flush with the surface of the opening 15 of the channel 5 facing into the first chamber.

[0160] Then, this form can also be: the second collection port 7 is slightly higher than the opening 15 of the channel 5, or the shoulder 14 of the second chamber is slightly higher than the opening of the channel 5. In this way, the liquid sample that has not been loaded into the second chamber 2 usually flows along this hierarchical structure from high to low into the first chamber 1 outside the channel 5.

[0161] Alternatively, an inclined surface that gradually slopes downward from the inside to the outside is formed between the surface of the second collection port 7 and the opening 15 of the channel 5, or an inclined surface that gradually slopes downward from the inside to the outside is formed between the shoulder 14 of the second chamber and the surface of the opening 15 of the channel 5. Then, this form of the inclined surface is more conducive to the liquid sample that has not been loaded into the second chamber 2 flowing along it and entering the first chamber 1.

[0162] The separation part is embodied in the form of the channel 5. The channel 5 is a component with two open ends, one communicating with the outside and the other communicating with the inside of the first chamber 1. Since the second chamber 2 can be assembled in it, in some cases, the second chamber 2 may enter the first chamber 1 along the channel 5. This situation must be absolutely avoided. On the one hand, the leaked part of the second chamber 2 will contaminate the liquid sample in the first chamber 1. On the other hand, if the second chamber 2 is not plugged or is pulled out, the channel 5 will become a leak in the first chamber, and a large amount of the liquid sample will leak from it. Therefore, it is necessary to set a limiting structure for the inward movement of the second chamber 2.

[0163] As Figure 2 、 7 As shown in 8 or 9, an external step surface 8 is provided in the channel 5. Taking this external step surface 8 as the dividing line, the inner diameter of the part of the channel 5 in the direction of the first chamber 1 near the external step surface 8 is smaller than the inner diameter of the part of the channel 5 in the direction of the outside near the external step surface 8. In this way, a structure with a smaller inner and larger outer diameter is formed on the inner wall of the channel 5, and this external step surface 8 is exactly the dividing line of the size. That is to say, the external step surface 8 can be used as a limiting structure.

[0164] To better adapt to this limiting structure, an internal step surface 9 can also be provided on the outer wall of the second chamber 2. Taking this internal step surface 9 as the dividing line, the inner diameter of the part of the outer wall of the second chamber in the direction of the inside of the first chamber 1 near the internal step surface 9 is smaller than the inner diameter of the part of the outer wall of the second chamber in the direction of the outside near the internal step surface 8. That is to say, the internal step surface 9 can cooperate with the external step surface 8 to limit the inward movement of the second chamber 2. In the initial state, the second chamber 2 is assembled in the first chamber 1. At this time, the internal step surface 8 and the external step surface 9 are usually in contact and fit. When the internal step surface 8 and the external step surface 9 are in contact and fit, the bottom of the second chamber 2 is flush with the surface of the outer opening of the channel 5. After that, as the sealing progresses, the internal step surface 8 and the external step surface 9 will separate, and the second chamber 2 will gradually be withdrawn outward.

[0165] The above is only one implementation form of this limiting structure. It should be understood that the limiting structure is a conventional mechanical structure that can be expanded and applied. As long as it can prevent the second cavity 2 from moving inward without restriction, it belongs to this category, for example, retaining rings, elastic parts, etc., which will not be listed here one by one.

[0166] In some preferred embodiments, a sealing structure can also be provided on the assembly surface of the second cavity and the channel. The so-called assembly surface refers to the outer surface of the second cavity and the inner surface of the channel. The sealing structure can be in the form of a sealing retaining ring 37. The sealing retaining ring 37 can limit the assembly of the second cavity and the channel, that is, it plays the same role as the aforementioned limiting structure, and can also play a sealing role in the separation of the second cavity and the channel. In some preferred embodiments, the sealing retaining ring adopts an O-ring. In some preferred embodiments, the sealing retaining ring 37 adopts a brush structure. When the second cavity exits the channel, the brush structure can scrape the surface of the second cavity to scrape off the sample that may be attached thereto. Of course, the O-ring can also play the above role.

[0167] In some preferred embodiments, the sealing structure and the limiting structure may not be arranged on the assembly surface of the channel and the second cavity, as long as they can play the above-mentioned role. The assembly position of the structure is not within the scope of the present invention.

[0168] Sample collection method

[0169] The present invention provides a method for collecting the aforementioned samples, the method adopts a sample collection device having the aforementioned first cavity 1 and second cavity 2, the first cavity 1 is used to collect liquid samples, the first cavity 1 has a sample inlet 4, the second cavity 2 is used to collect and confirm the detection of liquid samples, the detection timing and detection purpose of the first cavity 1 and the second cavity 2 are different, but the detection is for samples collected from the same batch. In order to achieve the same batch collection and multiple detection, the first cavity 1 and the second cavity 2 can be combined and separated, the same batch is collected when combined, and separate detection is performed after separation, or separation is performed after detection, and the second cavity 2 enters the secondary detection program alone. The sample detection device also includes a first sealing element 10 for sealing the first cavity 1, a second sealing element 11 for sealing the second cavity 2, and a third sealing element 16 for sealing the separation between the first cavity 1 and the second cavity 2, the separation being the aforementioned channel 5.

[0170] In some preferred embodiments, during the process of the first sealing element 10 sealing the first chamber 1, the second chamber 2 can be sealed by the second sealing element 11. In some preferred embodiments, during the process of the first sealing element 10 sealing the first chamber 1, the channel 5 can be sealed by the third sealing element 16. In some preferred embodiments, one of the second sealing element 11 and the third sealing element 16 is linked with the first sealing element 10 and the second sealing element 11 is linked with the third sealing element 16; or both the second sealing element 11 and the third sealing element 16 are linked with the first sealing element 10. In some preferred embodiments, before all the sealing processes, the channel 5 is blocked by the second chamber 2, and at this time, the first chamber 1 and the second chamber 2 form an integral body that is closed to the outside and connected inside. In some preferred embodiments, during the sealing process, the second chamber 2 moves outward relative to the channel 5.

[0171] In some preferred embodiments, the method for collecting a sample includes enabling the first chamber 1 and the second chamber 2 to be in liquid communication through the channel 5, and the liquid sample can flow from the first chamber 1 into the second chamber 2 through the channel 5 or a certain orifice (such as the opening 15) of the channel 5, so that while loading the liquid sample into the first chamber 1, the liquid sample can also naturally enter the second chamber 2. In some preferred embodiments, before the first chamber 1 and the second chamber 2 are separated, the channel 5 is sealed by the third sealing element 16. In some preferred embodiments, during the separation process of the first chamber 1 and the second chamber 2, the channel 5 is sealed by the third sealing element 16.

[0172] In some preferred embodiments, the method for collecting a sample includes the process of sealing the first chamber 1 and the second chamber 2 that have collected the sample. During the sealing process, the first chamber 1 and the second chamber 2 can move relative to each other so that a part of the second chamber 2 is pushed out of the first chamber 1. In some preferred embodiments, during the sealing process, a part of the second chamber 2 is exposed outside the first chamber 1. In some preferred embodiments, the sealing process of the first chamber 1 is accompanied by the sealing of the second chamber 2.

[0173] In some preferred embodiments, the method for collecting a sample includes the process of sealing the channel 5 that connects the first chamber 1 and the second chamber 2. During the sealing process, the first chamber 1 and the second chamber 2 can move relative to each other so that a part of the second chamber 2 is pushed out of the first chamber. In some preferred embodiments, during the sealing process, a part of the second chamber 2 is exposed outside the first chamber 1. In some preferred embodiments, the sealing process of the first chamber 1 is accompanied by the sealing of the channel 5. In some preferred embodiments, the sealing process of the channel 5 is after the sealing process of the second chamber 2. In some preferred embodiments, while sealing the channel 5, the second chamber 2 is pushed outwards.

[0174] Sample detection method

[0175] The present invention provides a method for detecting a collected sample, which is used to detect whether an analyte exists in the collected liquid sample. The detection method includes using the above-mentioned collection device or collection method to collect the sample to be detected. After a certain amount of sample is collected in the first chamber 1, the sample in the first chamber 1 is initially detected.

[0176] In some preferred embodiments, the second chamber 2 that has completed sample collection is first sealed, and then the sample in the first chamber 1 is detected by a test element. In some preferred embodiments, the first chamber 1 and the second chamber 2 are first separated, and then the sample in the first chamber 1 is detected by a test element. Since the liquid sample collected in the second chamber 2 is for secondary detection, separating the second chamber first can prevent the liquid sample in the second chamber for secondary detection from being contaminated by the test element in the initial detection. In some preferred embodiments, the channel connecting the first chamber 1 and the second chamber 2 is first sealed, and then the sample in the first chamber is detected by a test element. Sealing the channel prevents the test sample from leaking out.

[0177] In some preferred ways, a barrier element 31 capable of preventing the liquid sample from flowing into the detection area is provided in the first chamber 1. The barrier element 31 can open or close the entrance 32 connecting the first chamber 1 to the detection area, thereby preventing or releasing the liquid sample from flowing into the detection area 3. While sealing the channel 5, the third sealing element 16 triggers the barrier element 31 to open the entrance to the detection area. In some preferred embodiments, during the process of collecting the sample, the detection area 3 and the sample collection area (i.e., the interior of the first chamber) are isolated by the barrier element 31. After the sample collection is completed, the isolation between the detection area and the sample collection area by the barrier element 31 is removed, enabling the sample to enter the detection area.

[0178] In some preferred embodiments, a detection area 3 is provided in the first chamber 1. The detection area generally has the characteristic of being visible from the outside. For example, the detection area can be made of a transparent material. In some preferred embodiments, a test element is provided in the detection area 3. In some preferred embodiments, an entrance 32 that can be connected or closed is provided between the detection area 3 and the first chamber 1. In some preferred embodiments, the entrance 32 can cooperate with the barrier element 31 to be blocked and closed by the barrier element, or opened to connect the detection area and the first chamber.

[0179] Sealing element

[0180] As described above, when the first chamber 1 and the second chamber 2 are in the combined state, only one of the chambers can be sealed because they have a common opening and a passage 5 serves as a passage connecting the two. After the first chamber 1 and the second chamber 2 are separated, the second chamber 2 needs to be sealed independently so that the second chamber 2 can independently seal the liquid sample therein. Therefore, a sealing device specifically for the second chamber 2 is required. When the first chamber 1 and the second chamber 2 are separating, the first chamber 1 will inevitably generate a new opening (i.e., the opening 15 of the passage 5 to the outside) that may leak liquid in addition to the original opening (which will be sealed by the first sealing element 10). At this time, this opening 15 also needs to be sealed to prevent the liquid sample from flowing out. Therefore, a sealing device for the separation part is required.

[0181] For the above reasons, the present invention provides specific sealing structures for the first chamber 1 and the second chamber 2. These specific sealing structures include a first sealing element 10 for sealing the first chamber 1, a second sealing element 11 for sealing the second chamber 2, and a third sealing element 16 for sealing the separation part (i.e., the passage 5) of the first chamber 1 and the second chamber 2 when they are separated.

[0182] In some preferred ways, the first sealing element 10 and the mouth 6 of the first chamber 1 are sealed by a first thread structure 17, the second sealing element 11 and the mouth 7 of the second chamber 2 are sealed by a second thread structure 18, and the third sealing element 16 and the separation part (i.e., the passage 5) are sealed by a third thread structure 19.

[0183] As Figure 7The figure shows the state of the entire device before sealing. At this time, the first sealing element 10, the second sealing element 11 and the third sealing element 16 have not started to seal. When the user collects a suitable amount of liquid sample from the mouth 6 of the first cavity 1, the device can be sealed, and the first sealing element 10 is covered on the mouth 6 of the first cavity 1. The first sealing element 10 is rotated to cover the mouth 6 of the first cavity 1 to achieve the sealing of the first cavity 1. In the process of the first sealing element 10 sealing the first cavity 1, the second sealing element 11 seals the mouth 7 of the second cavity 2, or in the process of the first sealing element 10 sealing the first cavity 1, the third sealing element 16 seals the channel 5, or in the process of the first sealing element 10 sealing the first cavity 1, the second sealing element 11 and the third sealing element 16 seal the second cavity 2 and the channel 5 respectively. When When the first sealing element 10 completes the sealing of the first cavity 1, the second sealing element 11 and the third sealing element 16 have completed the sealing of the second cavity 2 and the channel 5 respectively, or, in the process of the first sealing element 10 sealing the first cavity 1, the second sealing element 11 seals the second cavity 2 as the first sealing element 10 rotates, and at the same time, the third sealing element 16 also rotates to seal the channel 5. The order of sealing completion is that the second sealing element 11 first completes the sealing of the second cavity 2. At this time, the second cavity 2 is assembled, forming a state in which the whole is still in the first cavity 1 but has actually been separated into an independent cavity by the second sealing element 11, and can be separated from the first cavity 1 at any time. Then the third sealing element 11 immediately completes the sealing of the channel 5, and the first sealing element 1 continues to rotate until the sealing of the first cavity 1 is completed.

[0184] It can be seen from the above description that the sealing process of the second sealing element 11 on the second cavity 2 must be carried out during the sealing process of the first sealing element 10 on the first cavity 1, and the sealing process of the third sealing element 16 on the channel 5 must also be carried out during the sealing process of the first sealing element 10 on the first cavity 1, while the sealing process of the second sealing element 11 on the second cavity 2 and the sealing process of the third sealing element 16 on the channel 5 can be carried out sequentially, partially overlapped, or completely synchronized.

[0185] Figure 8 The figure shows an intermediate process of sealing. Figure 8 In the state shown, the second sealing element 11 has completed the sealing of the second cavity 2, and the third sealing element 16 is about to start sealing the channel 5. The first sealing element 1 is in the process of sealing the first cavity 1. That is to say, in terms of the length of the thread, the length of the first thread structure 17 is equal to the sum of the lengths of the second thread structure 18 and the third thread structure 19. In this way, the first sealing element 10 can seal the first cavity 1.

[0186] It should be pointed out that in this sealing process, since the first sealing element 1, the second sealing element 2 and the third sealing element 3 are all sealed by tightening the threads, these sealing elements will inevitably have a height reduction during the thread matching process. This height reduction will push the second cavity 2 out of the first cavity 1 from the linkage point of view, so that a part of the second cavity 2 is exposed outside the first cavity 1, thereby facilitating the removal of the second cavity 2 from the first cavity 1. This will be described in detail in the linkage element below.

[0187] In some preferred embodiments, the sealing element can also seal each cavity in the form of a plug. For example, the first sealing element 1, the second sealing element 2 and the third sealing element 3 can all be in the form of a sealing plug.

[0188] In some preferred embodiments, a sealing ring can be provided on the inner wall of the channel or the outer wall of the second cavity. In some preferred embodiments, the sealing ring can adopt a flexible structure or a brush structure, and the function of the sealing ring is to prevent the second cavity or the separated sample from leaking out when the second cavity withdraws from the channel.

[0189] In some preferred embodiments, the sealing element only needs to include a second sealing element 11 for sealing the second cavity 2 and a third sealing element 16 for sealing the channel 5. The second sealing element and the third sealing element can work in conjunction to seal the second cavity and the channel, respectively.

[0190] In some preferred embodiments, the second sealing element 11 seals the second cavity 2 first, and then the third sealing element 16 seals the channel 5. In some preferred embodiments, the third sealing element 16 can move the second cavity 2 in a direction of separation from the first cavity 1 while sealing the channel 5. In some preferred embodiments, the second cavity 2 only needs to partially withdraw from the first cavity 1.

[0191] In some preferred embodiments, the second sealing element 11 is combined with the second cavity 2 in the process of sealing the second cavity 2. In some preferred embodiments, the third sealing element 16 is combined with the channel 5 in the process of sealing the channel 5, and at the same time presses the second cavity outwardly. The combination here means that the distance between the sealing element and the sealed component is getting closer and closer, or in the case of being assembled, the assembly is more tightly assembled. This assembly can further shorten the length or height of the entire assembly structure. For example, in a threaded structure, as the thread is screwed in, the height or length of the entire device will be shorter.

[0192] In some preferred embodiments, the second sealing element 11 can be detachably coupled or connected to the mouth of the second chamber 2. The coupling here is the same as the coupling described above. The second collection port refers to the collection port of the second chamber for collecting samples. Generally speaking, this collection port should also be the only opening of the second chamber. When this opening is closed, the second chamber will be in a sealed state.

[0193] In some preferred embodiments, the second sealing element 11 is a second cover. The second cover is detachably coupled or connected to the second collection port through a second threaded structure. In some preferred embodiments, the thread can be provided on the outer wall of the mouth of the second chamber and the inner wall of the second cover. In some preferred embodiments, the thread can be provided on the outer wall of the second cover and the inner wall of the mouth of the second chamber. The internal and external threads can be selected according to actual needs. The present invention is not limited to the connection method shown in the figure. In some preferred embodiments, the second sealing element is a second plug. The second plug can be tightly fitted with the inner wall of the mouth of the second chamber. By adopting the form of a plug and squeezing it inward to plug the mouth, the above-mentioned sealing effect can also be achieved, and the linkage between the plugs can also be realized during the insertion process of the plug. For the present invention, the sealing of the sealing element and the linkage during the sealing process are the solutions to be protected by the present invention.

[0194] In some preferred embodiments, the third sealing element can be detachably coupled or connected to the mouth of the channel. In some preferred embodiments, the third sealing element is a third cover. The third cover is detachably coupled or connected to the mouth of the channel through a third threaded structure. In some preferred embodiments, the third sealing element is a third plug. The third plug can be tightly fitted with the inner wall of the mouth of the channel. Similarly, the connection or coupling method between the third sealing element and the mouth of the channel can also adopt a structure similar to that in the previous paragraph. The present invention is not limited to the connection method shown in the figure. In some cases, different connection methods can also be selected for different covers according to needs, that is, a combined connection method can be adopted, which should also be within the protection scope of the present invention.

[0195] Linkage element

[0196] In order to achieve the sealing sequence of the first chamber 1, the second chamber 2, and the channel 5, a linkage structure needs to be provided for the first sealing element, the second sealing element, and the third sealing element. The function of the linkage structure is that when the first sealing element seals the first chamber, the second sealing element can also seal the second chamber. It is possible that when the first sealing element completes the sealing, the second sealing element also completes the sealing, or, before the first sealing element completes the sealing, the second sealing element has already completed the sealing.

[0197] These linkage structures include a first linkage element 20 for linking the first sealing element 10 and the third sealing element 16, and a second linkage element 21 for linking the second sealing element 11 and the third sealing element or the second sealing element 11 and the first sealing element 10.

[0198] The first linkage element 20 is a linkage rod. The two ends of the linkage rod are respectively connected to the first sealing element 10 and the third sealing element 16. When one of the first sealing element 10 and the third sealing element 16 rotates, it can drive the other one to rotate accordingly. This rotation accordingly can be synchronous or intermittent driving, but this rotation accordingly is usually in the same direction, but the possibility of the opposite direction is not excluded.

[0199] As a specific implementation form of the linkage rod, the first sealing element 10 is a cup cover, which has a relatively flat inner cover surface 22. An inner shaft hole 23 is provided on the inner cover surface 22. The upper end of the linkage rod is inserted into the inner shaft hole 23, as shown in Figure 2 、 7 、the part of the first sealing element 10 shown in 8. In some cases, the third sealing element 16 is also a cover structure, which has a relatively flat outer cover surface 24. An outer shaft hole is provided on the outer cover surface 24. The lower end of the linkage rod is inserted into the outer shaft hole. In this way, the linkage rod can link the first sealing element 10 and the third sealing element 16 through the inner and outer shaft holes, so that they can rotate synchronously. Then, when the user uses it, only by rotating the outermost first sealing element 10, the third sealing element 16 can be driven synchronously to seal the channel 5.

[0200] Or, the part of the third sealing element 16 can also be in the form shown in Figure 2 、 7 、8. A linkage hole 25 is provided at the lower end of the linkage rod. A coupling pin 26 is provided on the outer cover surface 24 of the third sealing element 16. The coupling pin 26 is inserted into the linkage hole 25 to realize the linkage between the third sealing element 16 and the linkage rod. In this way, the purpose of making the first sealing element 1 and the third sealing element 16 rotate synchronously can also be achieved. Or, the lower end of the linkage rod is directly fixed to the outer cover surface 24 of the third sealing element 16. In this case, the detachable separation and combination between the first linkage element 20 and the third sealing element 16 cannot be realized, but it will not affect the function of the device for collecting samples.

[0201] In any of the above implementation manners, the linkage rod passes through the first chamber 1 to connect the first sealing element 10 and the third sealing element 16. Moreover, the linkage rod or the connecting part thereon needs to have a certain shape, which is generally not a perfect circle, and can be, for example, square, semi-circular or triangular, etc. Correspondingly, the inner and outer shaft holes on the first sealing element 10 and the third sealing element 16 are also of the same shape. Then, the circumferential limit between the linkage rod and the inner and outer shaft holes is achieved through this shape, and relative rotation will not occur. In this way, the linkage rod can drive the first sealing element and the third sealing element to rotate through the two shaft holes, thus realizing linkage. In some other possible manners, the linkage rod and the inner and outer shaft holes can also be tightly fitted round holes. At this time, the force of linkage comes from the frictional force between the contacting surfaces of the linkage rod and the inner and outer shaft holes. In some other possible manners, the linkage rod and the shaft holes can also be in a stepped linkage cooperation relationship. Under this stepped linkage cooperation relationship, the linkage rod or the inner and outer shaft holes can rotate independently by a certain angle and then rotate synchronously.

[0202] Correspondingly, in the cooperation manner of the linkage hole 25 and the coupling pin 26, the linkage hole 25 and the coupling pin 26 can also adopt a similar shape as above to achieve linkage cooperation, or adopt a similar principle to achieve linkage cooperation.

[0203] As another implementation form of linkage cooperation, a shaft hole is provided on the linkage rod, and a cooperation element linked with the inner and outer shaft holes is provided on the first sealing element or the third sealing element. The cooperation element has the same shape as the shaft hole, and this shape is deviated from the axis of the linkage rod or is non-circular, so that the linkage rod and the cooperation element can achieve linkage. In some preferred manners, the linkage rod and the cooperation element can also be in a stepped linkage cooperation.

[0204] The second linkage element 21 can directly link the first sealing element 10 and the second sealing element 11, or can link the second sealing element 11 and the third sealing element 16. Which one to link is not a specific implementation scheme defined by the present invention. The purpose of the present invention is to be able to achieve the sealing priority order of the three. Such as Figure 2 、 7, in the form shown in Fig. 8, the second linkage element 21 is a linkage pin or a short object of a similar shape. The second linkage element 21 is fixedly connected to the upper cover surface 27 of the second sealing element. Of course, it can also be fixedly connected to the lower cover surface 28 of the third sealing element (this method is not shown in the figure). Whether the second linkage element 21 is fixedly connected to the second sealing element 11 or the third sealing element 16, a hole for cooperating with the second linkage element 21 is provided on the other one of the second sealing element 11 and the third sealing element 16 (that is, the one that is not fixedly connected to the second linkage element 21). Similarly, the second linkage element 21 and the hole cooperating therewith also have a certain shape, and this shape is similar to the aforementioned linkage rod. It can deviate from the rotation center of the second sealing element or the third sealing element, or it can be non-circular, such as square, semi-circular or triangular, etc. The purpose is to enable one of the linkage pin and / or the linkage hole to drive the other one to rotate therewith. This rotation therewith can be synchronous or intermittent driving. This rotation can be in the same direction or in the opposite direction.

[0205] When the second sealing element 11 is linked with the first sealing element 10, as the first sealing element 10 rotates, the second sealing element 11 can seal the second cavity 2 through the second thread structure 18. When the second sealing element 11 is linked with the third sealing element 16, since the third sealing element 16 is linked with the first sealing element 10, it will also drive the second sealing element 11 to seal the second cavity 2. Whether the direct linkage component of the second sealing element 11 is the first sealing element 10 or the third sealing element 16 can be considered according to the structural layout of the actual product and selected as needed.

[0206] In some preferred embodiments, the linkage structure includes a first sealing element for sealing the first chamber and a second sealing element for sealing the second chamber, and the first sealing element and the second sealing element are linked by a first linkage element. In some preferred embodiments, the linkage structure includes a first sealing element for sealing the first chamber and a third sealing element for sealing the passage, and the first sealing element and the third sealing element are linked by a first linkage element. In some preferred embodiments, the linkage structure includes a second sealing element for sealing the second chamber and a third sealing element for sealing the passage, and the second sealing element and the third sealing element are linked by a second linkage element. In some preferred embodiments, it includes a first sealing element for sealing the first chamber, a second sealing element for sealing the second chamber, and a third sealing element for sealing the passage, the first sealing element and the second sealing element are linked by a first linkage element, and the second sealing element and the third sealing element are linked by a second linkage element. In some preferred embodiments, it includes a first sealing element for sealing the first chamber, a second sealing element for sealing the second chamber, and a third sealing element for sealing the passage, the first sealing element and the second sealing element are linked by a first linkage element, and the second sealing element and the third sealing element are linked by a tight fit or a fixed connection.

[0207] In some preferred embodiments, the linkage between the second sealing element and the third sealing element can be achieved by a fixed connection. In some preferred embodiments, the linkage between the first sealing element and the third sealing element can be achieved by a fixed connection, that is, as Figure 13 shown, a fixed connection can be adopted between the second sealing element 11 and the third sealing element 16, which is equivalent to integrating the second sealing element 11 and the third sealing element 16 into one body, and naturally the linkage can be achieved. Such a fixed connection can be an integral connection itself, for example, a certain surface of the two sealing elements is fixedly connected or glued together, or it can be achieved through other components, such as a connecting rod. The present invention does not limit the way of fixed connection, as long as the effect of making them linked can be achieved. Similarly, the linkage between the first sealing element and the second sealing element, and between the first sealing element and the third sealing element can also be naturally achieved in this way.

[0208] In some preferred embodiments, the first linkage element is a linkage rod. In some preferred embodiments, the second linkage element is a linkage pin. In some preferred embodiments, the said linkage refers to synchronous rotation. In some preferred embodiments, the said linkage refers to synchronous movement in the direction close to the inside of the sample. In the present invention, the linkage especially means that the sealing process is synchronous. For example, synchronous rotation, which is especially applicable to the connection mode of the thread structure. Another example is synchronous insertion, which is especially applicable to the connection mode of the plug. In the connection modes such as snap connection and knob, a similar linkage structure can also be adopted.

[0209] Limit structure

[0210] As described above, the channel 5 is a form of implementation at the separation point, and its function is to allow the second chamber 2 to be plugged and installed therein and seal it. Then, as described above, it is necessary to limit the cooperation between the second chamber 2 and the channel 5. Otherwise, the second chamber 2 may slide into the first chamber 1 through this channel 5, causing contamination of the liquid sample or a large leakage of the liquid sample at the channel 5. Therefore, a limiting structure that cooperates with each other is provided on the inner wall of the channel 5 and the outer wall of the second chamber 2. This limiting structure limits the extreme position of the installation of the second chamber 2 to a certain position within the channel 5. Once the second chamber 2 is installed in place, it will no longer move inward.

[0211] As Figure 2 、 7 、as shown in 8, an outer step surface 36 is provided on the inner wall of the channel 5. Taking the outer step surface 36 as the demarcation line, the inner diameter of this part of the channel 5 close to the first chamber 1 is larger than the inner diameter of this part close to the outside. At the same time, as Figure 5 - 6 shown, an inner step surface 29 that cooperates with this outer step surface 36 is provided on the outer wall of the second chamber 2. Taking the inner step surface 29 as the demarcation line, the inner diameter of this part of the second chamber 2 close to the first chamber 1 is smaller than the inner diameter of this part close to the outside. Then, the cooperation between the inner step surface 29 and the outer step surface 36 can limit the extreme installation position of the second chamber 2 at this cooperation point. When the second chamber 2 is inserted, due to the engagement of the step surface, it cannot move further inward, realizing the limitation of the second chamber 2.

[0212] The channel 5 is a structure that enables the first chamber 1 to be assembled with the second chamber 2. The channel 5 can be regarded as a part of the first chamber 1. Ideally, after the second chamber 2 is installed, the outer contour (such as the bottom surface) of the second chamber 2 does not exceed the outer surface of the channel 5. This situation can make the first chamber 1 and the second chamber 2 form a whole before use. However, if this is still the case during use, it will be difficult to remove the second chamber 2 from the first chamber 1. Therefore, the linkage structure of the present invention can also realize that when the sealing element seals the first chamber 1, the second chamber 2 and the channel 5, it can gradually push the second chamber 2 outwards. When all the sealing processes (including the sealing of the first chamber 1 by the first sealing element 10, the sealing of the second chamber 2 by the second sealing element 11, and the sealing of the channel 5 by the third sealing element 16) are completed, a part of the second chamber 2 extends beyond the outer contour of the first chamber 1 (as Figure 2 shown). At this time, the second chamber 2 can be separated from the first chamber 1 through this extended part. The second chamber 2 sealed by the second sealing element 11 is a sealed cavity, and the liquid sample therein can be used for secondary confirmation testing. After the second chamber 2 is separated from the first chamber 1, it can be independently transported to the secondary detection mechanism.

[0213] In order to realize that the second cavity 2 is gradually pushed out during the sealing process, the lengths of the threaded structure and the linkage element need to meet certain requirements. For example, as described above, the second cavity 2 is first sealed by the second sealing element 11, and then immediately followed by the channel 5 being sealed by the third sealing element 16. During the sealing process of these two, the first sealing element 11 is always in the process of sealing the first cavity 1. Ideally, when the first sealing element 11 completes the sealing, the third sealing element 16 also just completes the sealing. In this case, the length of the first threaded structure 17 is equal to the sum of the lengths of the second threaded structure 18 and the third threaded structure 19. The length of the linkage rod satisfies that when the first sealing element 1 rotates downward until the second sealing element 11 just completely seals the second cavity 2, the relative position of the second cavity 2 and the channel 5 remains unchanged. When the third sealing element 16 starts to seal the channel 5, the second cavity 2 starts to be pushed outwards. That is to say, in the initial stage of closing, the first sealing element 11 drives the second sealing element and the third sealing element to rotate through the linkage rod. At this time, the third sealing element is in an idling state and does not contact other components, while the second sealing element has started to cooperate with the threaded structure on the second cavity 2. After the second cavity 2 is sealed, continue to rotate the first sealing element 11. At this time, the first threaded structure 17 continues to close, and through the linkage rod, the third sealing element and the sealed second cavity are pushed downward. While the second cavity 2 starts to move outwards along the channel 5, the third sealing element starts to seal the channel 5 until the channel 5 is completely sealed. At this time, the first sealing element 11 also just completes the sealing of the first cavity 1.

[0214] Since it is impossible to completely seal between the second sealing element 11 and the third sealing element 16, there will inevitably be a certain gap 30. This part of the gap may enter the liquid sample when the second sealing element 11 seals the second cavity. Therefore, ideally, after the second sealing element 11 completes the sealing, the third sealing element 16 will still idle for a short distance to squeeze out the liquid in the gap, and then seal the channel 5. This will make the liquid sample remaining in the gap as little as possible and reduce the leakage of the liquid sample during separation. In this case, the length of the first threaded structure 17 needs to be slightly greater than the sum of the lengths of the second threaded structure 18 and the third threaded structure 19. In practice, the specific dimensions can be adjusted according to needs. The main technical solution to be achieved by the present invention lies in the linkage relationship of the sealing and the sealing sequence.

[0215] Barrier element

[0216] In addition to the above structure, the initial inspection of samples using the device of the present invention is also an aspect of the present invention. In an ideal state of the present invention, it is hoped that after the sample collection is completed, especially after the liquid sample for secondary confirmation testing in the second chamber 2 is collected and sealed, the initial inspection can be carried out. In this way, the test sample for secondary confirmation will not be contaminated by the initial inspection, ensuring the accuracy of the secondary test. For this purpose, the present invention also provides a barrier element 31. As mentioned before, the function of the barrier element is to temporarily separate the test element from the liquid sample collected in the initial detection chamber (i.e., the first chamber 1), temporarily preventing the liquid sample from entering the detection area 3. Of course, the barrier element can also release the sample. Therefore, the barrier element is a component used to block or open the connection between the first chamber 1 and the detection area 3. In the initial state of sample collection, the barrier element 31 separates the first chamber 1 from the test area 3. At this time, the liquid sample cannot enter the detection area 3, and the test element will not come into contact with or react with the liquid sample. When a certain amount of sample is collected or at a certain time, the barrier element 31 opens the detection entrance 32 connecting the first chamber 1 to the detection area 3, allowing a part of the liquid sample to enter the detection area 3 from the detection entrance 31 and performing an initial inspection through the test element to obtain a preliminary test result.

[0217] As Figure 2 - 4 shown, the barrier element 31 can be a rotating piece that can rotate around the axis of the first chamber 1. A baffle 33 can be provided on the rotating piece. Before use and in the initial state of sample collection, the baffle 33 can block the entrance 32. At this time, even if the liquid sample enters the first chamber 1 and the second chamber 2, it is impossible to enter the detection area 3 and will not come into contact with the test element. Then, during the rotation of the sealing element, the barrier element 31 can interfere with one of the sealing elements at an appropriate time to open the entrance 32, allowing the liquid sample for the initial inspection to enter the detection area 3 for the initial inspection.

[0218] For example, the barrier element 31 is linked with the second sealing element. When the second sealing element completes the sealing of the second chamber, it touches the barrier element to open the entrance connecting to the detection area. At this time, the sealing of the second chamber is completed, ensuring that the liquid sample in the second chamber will not have any contact with the sample in the detection area and guaranteeing the accuracy of the secondary test.

[0219] Or, as Figure 2 - 4As shown, the barrier element 31 is sleeved on the periphery of the channel 5 and can rotate around it. The third sealing element 16 is provided with a protrusion 34. When the third sealing element 16 is sealed with the channel 5, the protrusion 34 can be lowered to a height that interferes with the baffle 33. When the third sealing element 16 completes the sealing of the channel 5, the protrusion 34 interferes with the baffle 33, pushing the baffle 33 to drive the barrier element 31 to rotate, opening the entrance 32 connected to the detection area. Since the second sealing element has completed the sealing of the second cavity during the process of the third sealing element sealing the separation point, when the third sealing element completes the sealing of the separation point, the sealing of the second cavity has been completed first. Therefore, when the entrance of the detection area is opened at this time, the liquid sample in the second cavity will not have any contact with the sample in the detection area, which can ensure the accuracy of the secondary detection.

[0220] In some preferred embodiments, the barrier element is used to open a detection area for detecting an initial test sample. When the second cavity does not collect a sample or is collecting a sample, the detection area is closed. When the collection of the second cavity is completed and sealed, the sample can enter the detection area.

[0221] In some preferred embodiments, a second sealing element for sealing the second cavity is included, and the second sealing element can open or close the detection entrance. When the second sealing element completes the sealing of the second cavity, the detection entrance is opened. In some preferred embodiments, the second sealing element is linked with the blocking element. When the second sealing element completes the sealing of the second cavity, the blocking element is triggered to open the entrance connected to the detection area.

[0222] In some preferred embodiments, the first cavity can be connected to or blocked by liquid from the detection area. When the detection entrance is opened, the first cavity and the detection area are connected by liquid, and when the detection entrance is closed, the first cavity and the detection area are blocked. In some preferred embodiments, the first sealing element is linked with the second sealing element, and the first sealing element can open or close the detection entrance. When the second sealing element completes the sealing of the second cavity, it drives the first sealing element to open the detection entrance. In some preferred embodiments, the first sealing element is linked with the blocking element. When the second sealing element completes the sealing of the second cavity, the first sealing element triggers the blocking element to open the entrance to the detection area.

[0223] In some preferred embodiments, the third sealing element is linked with the second sealing element, and the third sealing element can open or close the detection entrance. When the second sealing element completes the sealing of the second cavity, the third sealing element is driven to open the detection entrance. In some preferred embodiments, the third sealing element is linked with the blocking element. When the second sealing element completes the sealing of the second cavity, the third sealing element triggers the blocking element to open the entrance to the detection area.

[0224] A specific implementation scheme

[0225] Before use, the linkage rod and the first sealing element 10, the second sealing element 11, and the third sealing element 16 can all be in a separated or partially separated state. At this time, the first sealing element 10 in the separated state can be used to close the entire device, and the second sealing element 11, the third sealing element 16, and the linkage rod in the separated state can all be placed into the first chamber 1 as a complete product package.

[0226] During use, open the first sealing element 10 and take out the accessories (including the second sealing element, the third sealing element, and the linkage rod). In some cases, these accessories can also be separately packaged in other ways. The present invention does not limit the packaging method of the accessories. Pour the liquid sample into the device through the first collection port. At this time, a part of the liquid sample will enter the second chamber at the same time. Since the second chamber is located at the bottom of the first chamber, generally, the second chamber will be filled with the liquid sample first. Continue to pour the liquid sample into the device until the required amount is reached.

[0227] After the liquid sample collection is completed, start to seal the device. Connect the relevant accessories together as Figure 7 shown, and then rotate the first sealing element 10 to seal the first chamber. During this process, as Figure 8 shown, the linkage rod first drives the second sealing element and the third sealing element to move downward until the second sealing element contacts the second collection port. Further rotation will enable the second sealing element to seal the second chamber. While sealing the second chamber, due to the linkage relationship of the linkage element, the sealing elements as a whole moving integrally continue to move downward until the second sealing element completes the sealing. Further, the first sealing element continues to rotate downward. At this time, the third sealing element starts to seal the channel. Since the second sealing element and the third sealing element or the first sealing element are still in a linkage state, the second sealing element will drive the sealed second chamber to rotate and move downward at the same time. At this time, the downward movement of the second chamber is equivalent to being pushed outwards relative to the channel, and the bottom of the second chamber gradually exposes outside the bottom of the first chamber until the sealing of the channel is completed. At this time, the sealing of the first chamber is also completed. To prevent excessive rotation, a sealing limit element is provided outside the first collection port. This sealing limit element is as Figure 2 、 4, as shown in FIGS. 7 or 8, it can be a limiting ring 35. The outer periphery of this limiting ring is larger than that of the first sealing element. Therefore, when the first sealing element rotates to contact this limiting ring, it can no longer move downward. The sealing and limiting structure can protect the internal structure and prevent damage to the internal structure caused by excessive rotation. Of course, this sealing and limiting element can also be a limiting block or a buckle. Alternatively, the sealing and limiting structure can be realized by the end point of the thread. When the first sealing element rotates to the end point of the first thread structure, it is naturally limited.

Claims

1. Sample collection and detection device, comprising: A first chamber for collecting a liquid sample, a second chamber for collecting and confirming the detection of the liquid sample, and the first chamber and the second chamber can be combined or separated; The sample detection device further includes a first sealing element for sealing the first chamber, a second sealing element for sealing the second chamber, and a third sealing element for sealing the separation between the first chamber and the second chamber; The separation includes a channel, and the second chamber can move within the channel; there is an outer stepped surface within the channel, and the inner diameter of the part of the channel closer to the first chamber in the direction of the outer stepped surface is smaller than the inner diameter of the part of the channel closer to the outside in the direction of the outer stepped surface, forming a structure with a smaller inner and larger outer diameter on the inner wall of the channel; an inner stepped surface is also provided on the outer wall of the second chamber, and the inner diameter of the part of the outer wall of the second chamber closer to the inside of the first chamber in the direction of the inner stepped surface is smaller than the inner diameter of the part of the outer wall of the second chamber closer to the outside in the direction of the inner stepped surface, and the inner stepped surface and the outer stepped surface form a fit to limit the inward movement of the second chamber. In the initial state, the second chamber is assembled in the first chamber, and at this time, the inner stepped surface and the outer stepped surface are in contact fit. When the inner stepped surface and the outer stepped surface are in contact fit, the bottom of the second chamber is flush with the surface of the outer opening of the channel; during the process of the first sealing element sealing the first chamber, the second chamber can be sealed by the second sealing element; afterwards, as the sealing progresses, the inner stepped surface and the outer stepped surface will separate, and the second chamber will gradually be withdrawn outwards.

2. The sample collection and detection device according to claim 1, wherein When the first chamber and the second chamber are combined, the first chamber and the second chamber can be in a liquid communication state.

3. The sample collection and detection device according to claim 1, wherein After the second sealing element seals the second chamber, the third sealing element seals the separation.

4. The sample collection and detection device according to claim 1, wherein After the second sealing element completes the sealing of the second chamber, the third sealing element completes the sealing of the separation.

5. The sample collection and detection device according to claim 1, wherein During the process of the first sealing element sealing the first chamber, the separation can be sealed by the third sealing element.

6. The sample collection and detection device according to claim 1, wherein One of the second sealing element and the third sealing element is linked with the first sealing element and the second sealing element is linked with the third sealing element.

7. The sample collection and detection device according to claim 1, wherein Both the second sealing element and the third sealing element are linked with the first sealing element.

8. The sample collection and detection device according to claim 6 or 7, wherein The sample detection device further includes a first linkage element for linking the first sealing element and the third sealing element.

9. The sample collection and detection device according to claim 6 or 7, wherein The linkage means synchronous rotation.

10. The sample collection and detection device according to claim 6 or 7, wherein The linkage means synchronous movement in the direction closer to the inside of the sample.

11. The sample collection and detection device according to claim 8, wherein a limiting structure of the second chamber is provided in the channel.

12. The sample collection and detection device according to claim 11, wherein after the second chamber is sealed, it can move along the channel in a direction away from the first chamber.

13. The sample collection and detection device according to claim 1 or 4, wherein it includes a detection area for initially collecting samples, and the detection area can be opened or closed.

14. The sample collection and detection device according to claim 13, wherein when the second chamber has not collected samples or is collecting samples, the detection area is closed, and when the collection in the second chamber is completed and sealed, the sample can enter the detection area.

15. The sample collection and detection device according to claim 14, wherein it further includes a barrier element, and the barrier element can open or close the detection entrance.

16. The sample collection and detection device according to claim 15, wherein the barrier element can be linked with the third sealing element and open the detection area entrance under the drive of the third sealing element.

17. The sample collection and detection device according to claim 16, wherein the barrier element can be linked with the second sealing element and open the detection area entrance under the drive of the second sealing element.

18. The sample collection and detection device according to claim 17, wherein the barrier element can be linked with the first sealing element and open the detection area entrance under the drive of the first sealing element.

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