Mouse Neural Retrograde Tracing Labeling Kit

Abstract

A mouse nerve retrograde tracing labeling component comprises a cylindrical trough body and an upper cover, wherein the cylindrical trough body is cylindrical in shape, the cross section of the cylindrical trough body is a major arc, the upper cover is an arc-shaped plate and the cross section of the cylindrical trough body is a minor arc, the upper cover is buckled on the cylindrical trough body, and the upper cover and the cylindrical trough body form a complete cylindrical shape; in summary, the present invention can realize single or even multiple retrograde tracing labeling of mouse nerves, thereby making it possible to directly observe the relationship between motor neuron pools or neuron subgroups from different sources in the spinal cord.

Description

Technical Field

[0001] The invention belongs to the technical field of neurobiological science experiments, and in particular relates to a mouse nerve retrograde tracing labeling component. Background Art

[0002] Neural retrograde tracing is a commonly used technique in neurobiological research. This technique can be used to determine the source, number and distribution of neuronal cell bodies projecting to a specific target area. There are many methods for labeling neural retrograde tracing, including soaking the nerve stump in a tracer (i.e., dye) or injecting the dye directly into the nerve trunk or target muscle. However, the method of injecting dyes into the nerve trunk or target muscle will result in low efficiency of neuron labeling and large variability in the number of labeled cells. In addition, the nerve stump soaking method, such as the traditional small pool method and the recently emerged silicone cap method, is introduced in the Journal of Neuroscience Methods, No. 259, 2016, "Retrograde labeling of regenerating motor and sensory neurons using silicone caps". However, it is still difficult to operate for single tracer labeling of mouse nerves, and even more difficult to achieve for multiple tracer labeling (compared with single tracer technology, multiple tracer technology can more intuitively observe the relationship between different neuronal subpopulations in the spinal cord, thereby greatly reducing the number of experimental mice required). Summary of the invention

[0003] In order to solve the deficiencies in the prior art, the present invention provides a mouse nerve retrograde tracing labeling component with reasonable structural design and convenient operation.

[0004] In order to solve the above technical problems, the present invention adopts the following technical solutions: a mouse nerve retrograde tracing labeling component, comprising a cylindrical trough body and an upper cover, the cylindrical trough body is cylindrical in shape, the cross section of the cylindrical trough body is a major arc, the upper cover is an arc-shaped plate and its cross section is a minor arc, the upper cover is buckled on the cylindrical trough body, the upper cover and the cylindrical trough body form a complete cylindrical shape, the front and rear ends of the cylindrical trough body are respectively fixed with circular blocking plates, the front of the cylindrical trough body is fixed with a circular partition, the partition divides the inner cavity of the cylindrical trough body into a placement groove on the front side and a storage groove on the rear side, the partition and the front blocking plate are both provided with notches, and the two notches are arranged correspondingly in front and back;

[0005] A fixing needle is fixed vertically upward at the bottom of the storage tank of the cylindrical tank body, and two liquid and gas holes are arranged on the upper cover, and the liquid and gas holes are correspondingly located above the storage tank.

[0006] Binding columns are fixed on the left and right sides of the cylindrical trough body and the left and right sides of the upper cover. The two binding columns on the upper cover are arranged one by one corresponding to the two binding columns on the cylindrical trough body, and the two binding columns on the cylindrical trough body are connected with binding belts.

[0007] The binding straps are either metal wire or microscopic sutures.

[0008] The length of the fixing pin is less than 1 mm.

[0009] The diameter of the liquid-gas pore is less than 1 mm.

[0010] The number of liquid-gas pores is at least two.

[0011] By adopting the above technical scheme, the present invention has the following beneficial effects: the present invention can conveniently place the nerve stump therein through the arrangement of the cylindrical trough body and the upper cover, and the partition can limit the displacement of vaseline to the nerve stump, thereby affecting the nerve stump's uptake of dye; the fixing needle can effectively prevent the displacement of the nerve stump, thereby preventing the nerve stump from slipping out of the component; the cylindrical trough body and the upper cover can be quickly fixed together through the pre-set binding belt (such as metal wire or 12-0 micro suture) on the binding column, thereby preventing the upper cover and the cylindrical trough body from relative displacement, resulting in dye leakage and contamination of surrounding tissues; the liquid-gas hole helps to discharge bubbles or excess dye, and dye can also be supplemented through the liquid-gas hole.

[0012] In summary, the present invention can achieve single or even multiple retrograde tracing labeling of mouse nerves, thereby making it possible to directly observe the relationship between motor neuron pools or neuron subpopulations from different sources in the spinal cord. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a structural schematic diagram of a cylindrical tank body;

[0014] Figure 2 It is a structural schematic diagram of the cylindrical tank from another angle;

[0015] Figure 3 It is a schematic diagram of the structure of the upper cover. DETAILED DESCRIPTION

[0016] like Figure 1-3As shown, the mouse nerve retrograde tracing labeling component of the present invention comprises a cylindrical tank body 1 and an upper cover 2. The cylindrical tank body 1 is cylindrical in shape, and the cross section of the cylindrical tank body 1 is a major arc. The upper cover 2 is an arc-shaped plate and its cross section is a minor arc. The upper cover 2 is buckled on the cylindrical tank body 1, and the upper cover 2 and the cylindrical tank body 1 form a complete cylindrical shape. The front and rear ends of the cylindrical tank body 1 are respectively fixed with circular blocking plates 3. The front of the cylindrical tank body 1 is fixed with a circular partition 4. The partition 4 divides the inner cavity of the cylindrical tank body 1 into a placement groove 5 on the front side and a storage groove 6 on the rear side. The placement groove 5 is used to place nerves and vaseline. The storage groove 6 is a main tank body for storing dyes. The partition 4 and the front blocking plate 3 are both provided with notches 7, and the two notches 7 are arranged correspondingly in the front and rear.

[0017] A fixing needle 8 is fixed vertically upward at the bottom of the storage tank 6 of the cylindrical tank body 1, and the length of the fixing needle 8 is less than 1 mm. Binding columns 9 are fixed on the left and right sides of the cylindrical tank body 1 and the left and right sides of the upper cover 2. The two binding columns 9 on the upper cover 2 are arranged one by one corresponding to the two binding columns 9 on the cylindrical tank body 1. The two binding columns 9 on the cylindrical tank body 1 are connected with binding belts (such as metal wires or 12-0 microscopic sutures). At least two liquid-gas holes 10 are opened on the upper cover 2. The diameter of the liquid-gas holes 10 is less than 1 mm. The liquid-gas holes 10 are correspondingly located above the storage tank 6. The arrangement of the liquid-gas holes 10 facilitates the micro-syringe to inject dye or extract bubbles.

[0018] The specific working process of the present invention is as follows: first, a circle of vaseline is applied on the upper edge of the cylindrical tank body 1, and vaseline is applied in the placement groove 5 of the cylindrical tank body 1, and then the nerve stump is carefully pressed into the two notches 7 using micro forceps (the position of the nerve stump should pass over the fixing needle 8 so that the fixing needle 8 can fix the nerve stump), and the nerve in the placement groove 5 is ensured to be covered and wrapped by vaseline, and dye is injected into the storage groove 6 using a micro syringe, and the upper cover 2 is placed on the cylindrical tank body 1, and the upper cover 2 is gently pressed so that the upper cover 2 is well buckled on the cylindrical tank body 1, and a binding belt (such as a metal wire or a 12-0 micro suture) is passed around the binding column 9 on the upper cover 2 and then interlocked or knotted to fix it, and a gauze strip (or a cotton swab or a gauze thread) is used to remove excess dye overflowing from the liquid-gas hole 10, and the liquid-gas hole 10 is blocked with vaseline, and multiple tracer markings of mouse nerves can be achieved by using multiple marking components.

[0019] This embodiment does not impose any formal limitation on the shape, material, structure, etc. of the present invention. Any simple modification, equivalent change and modification made to the above embodiment based on the technical essence of the present invention shall fall within the protection scope of the technical solution of the present invention.

Claims

1. A mouse neuron retrograde tracing and labeling component, characterized in that: It includes a cylindrical trough body and an upper cover. The outer shape of the cylindrical trough body is cylindrical, and the cross section of the cylindrical trough body is a major arc. The upper cover is an arc-shaped plate and its cross section is a minor arc. The upper cover is buckled on the cylindrical trough body. The upper cover and the cylindrical trough body form a complete cylindrical shape. The front and rear ends of the cylindrical trough body are respectively fixed with circular plugging plates. The front side of the cylindrical trough body is fixed with a circular partition. The partition divides the inner cavity of the cylindrical trough body into a placement groove on the front side and a storage groove on the rear side. Notches are provided on the partition and the front plugging plate. The two notches are correspondingly arranged front and back. A fixing needle is fixed vertically upward at the bottom of the storage groove of the cylindrical trough body. Two liquid and gas holes are provided on the upper cover. The liquid and gas holes are correspondingly located above the storage groove. Binding columns are fixed on the left and right sides of the cylindrical trough body and the left and right sides of the upper cover. The two binding columns on the upper cover are arranged one by one corresponding to the two binding columns on the cylindrical trough body. The two binding columns on the cylindrical trough body are connected with binding belts; the binding belts are metal wires or microscopic sutures; the length of the fixing needle is less than 1mm.

2. The mouse neural retrograde tracing labeling assembly according to claim 1, characterized in that: The diameter of the liquid-gas pore is less than 1 mm.

3. The mouse neural retrograde tracing labeling assembly according to claim 1, characterized in that: The number of liquid-gas holes is at least two.

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